LOCUS       NZ_CP091818             4018 bp    DNA     circular CON 25-SEP-2023
DEFINITION  Klebsiella pneumoniae strain 10MC160002271 plasmid pKP_FQ61_6,
            complete sequence.
ACCESSION   NZ_CP091818
VERSION     NZ_CP091818.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15813776
            Assembly: GCF_025144525.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Tsui,C.K.-M., Abid,F.B., McElheny,C.L., Almuslamani,M., Omrani,A.S.
            and Doi,Y.
  TITLE     Genomic epidemiology revealed the emergence and worldwide
            dissemination of ST383 carbapenem-resistant hypervirulent Klebsiella
            pneumoniae and hospital acquired infections of ST196 Klebsiella
            quasipneumoniae in Qatar
  JOURNAL   bioRxiv (2022)
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Tsui,K.M., Ben Abid,F., Omrani,A.S. and Doi,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2022) Pathology, Sidra Medicine, POBox26999, Doha,
            Qatar
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: NOV-2021
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: FQ61_unicyler_hybrid.assembly
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/25/2023 02:39:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,786
            CDSs (total)                      :: 5,660
            Genes (coding)                    :: 5,488
            CDSs (with protein)               :: 5,488
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 66 of 172
            Pseudo Genes (incomplete)         :: 105 of 172
            Pseudo Genes (internal stop)      :: 33 of 172
            Pseudo Genes (multiple problems)  :: 26 of 172
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091818.1.
            Bacteria and source DNA available from Hamad Medical Corporation.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="10MC160002271"
                     /isolation_source="pus"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP_FQ61_6"
                     /geo_loc_name="Qatar:Doha"
                     /lat_lon="25.28 N 51.53 E"
                     /collection_date="2016-04-28"
                     /collected_by="Hamad Medical Corporation"
     gene            complement(join(3582..4018,1..91))
                     /locus_tag="L7F76_RS28835"
                     /old_locus_tag="L7F76_28780"
     CDS             complement(join(3582..4018,1..91))
                     /locus_tag="L7F76_RS28835"
                     /old_locus_tag="L7F76_28780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016246513.1"
                     /translation="MKCFWACSVSVVLAMSFTVAAAENNDNVTYTKPSAGRDLYLTCKG
                     YQVKGASVINVEKPSYLEGTGEQVEFAIITPVGASDGLKVFFDSWGFSSFGLIESHVMH
                     EHWSLVHVGGRASVKGEKIDYSFVDNQPQEGGSYKPKTGVFLYGCMNSDKRPSWVEKIY
                     TRTGVFRGGIED"
                     /name="hypothetical protein"
     gene            complement(220..435)
                     /locus_tag="L7F76_RS28840"
                     /old_locus_tag="L7F76_28785"
     CDS             complement(220..435)
                     /locus_tag="L7F76_RS28840"
                     /old_locus_tag="L7F76_28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_072048928.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(439..924)
                     /locus_tag="L7F76_RS28845"
                     /old_locus_tag="L7F76_28790"
     CDS             complement(439..924)
                     /locus_tag="L7F76_RS28845"
                     /old_locus_tag="L7F76_28790"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_061390563.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMISQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIQEQKSTQAMLSEKNNGVQLTTCGEERRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<959..1663)
                     /locus_tag="L7F76_RS28965"
                     /pseudo=""
     CDS             complement(<959..1663)
                     /locus_tag="L7F76_RS28965"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1602..1925)
                     /locus_tag="L7F76_RS28855"
                     /old_locus_tag="L7F76_28800"
     CDS             complement(1602..1925)
                     /locus_tag="L7F76_RS28855"
                     /old_locus_tag="L7F76_28800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024191642.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQICLDEKPSRAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQVVAVLMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2573..2686
                     /locus_tag="L7F76_RS28970"
     CDS             2573..2686
                     /locus_tag="L7F76_RS28970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072035093.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHDKTPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(3115..3327)
                     /locus_tag="L7F76_RS28860"
                     /old_locus_tag="L7F76_28805"
     CDS             complement(3115..3327)
                     /locus_tag="L7F76_RS28860"
                     /old_locus_tag="L7F76_28805"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_034169353.1"
                     /translation="MGDFERTFGAGADFDRIIDENNSEYFREDRRKTKNSKKKFSSFQD
                     ALVWAKANPGKTIIRSPDGVGFIEK"
                     /name="hypothetical protein"
     Typing          complement(2039..2216)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.222"
                     /coverage="83.0"
                     /evalue="4.5e-50"
     Typing          2163..2686
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.434"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(129..1612)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.717"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tatatgtaac gttgtcgtta ttctccgcag cagcgactgt aaatgacata gccaaaacca
       61 ccgagactga gcaagcccag aaacatttca taacgcatcc cttttcattg attaactaca
      121 ttccaagctc aaaacctttc tgccgctgcg atcgttccag ctccagcgct ttctggtgct
      181 gcgccgcgcg ctcctgctcc cggtgcgcct cgatgccatt cagccttgcg acaatcggcg
      241 cagctgatcg ctcaaactct gcacctgcct gctcaagccc gtgacgcgct cgctcagcgc
      301 cgcgttctcc cgttgcataa gcccagacat cgtccgccat tccgcgaagg cgttctccca
      361 ctcgtccagc cttttcgagt agtcctgctg tagctgctct aatgcgctca gcaactgttt
      421 ttccagttcc gtcatgtgct atttccccgc cagtatcatc cagctcgaat cctctccgaa
      481 tcttcccgct tccgggttca ccctcacgca gcggcgtctc tcctccccgc aggtcgtgag
      541 ctggacgccg ttgttcttct ctgaaagcat ggcctgcgtg ctcttctgtt cctggatggt
      601 cgtgtagttg tcgagtatct gttgcccctg ccaccacaga atgcccgcgc tcgaggcgat
      661 cagtagcgcg gagaccagga ctatggtcag ccacgtctgg ctgatcatac gcagcatccc
      721 tttcgtgcgt tggctcatgg ctgaggacag cttccggtcg tggtcgagga tggcggcgct
      781 gattctcttc tcgctttcgc tcagttccgc tctgacagac ttctcgtgct cgctgaatgc
      841 ggctttcagc atctcgccgg tagtctgctg ctgcgctttc gatttctgct ctaagtcctt
      901 cgccagcgtt aaaagactgt tcatagatgg ctcccttcag tcggatgttt cgccccccgt
      961 cagggtcggc aatgctgatg ctgcttttcg tggtgcgcac cacctcaaaa cccgcacttt
     1021 ccagcgcctc agtgacgtcc tgacgggttt tcagctcccc ggatgaggca agggaaagta
     1081 ggccgcgcgt aatcgcctgg gcggcttcct gcttcgcttc cggcagcgcg gacggtgtaa
     1141 ccagcgcccg ccggttttcc ggcgcgttcg ggtcgtgcag ccccagtctg ccgttcacta
     1201 tggtctgcca ggcatcaatg cgcggacggt cggcgcggtc gtaatacggc tggagacgtc
     1261 tgccggtcag cagctccgtg ttcgggatca ggaagttcag ctccagccgc cctttgtccc
     1321 ggtgctcaac ccacagtacg ctgtactggt ctttatcgag tccgggcatc agaacccgct
     1381 caaagctcgc catcagcttt tcacgctggc cgggcggcaa atcctgttcg gcaaaagaca
     1441 gcacgccgga ggtgtatttc ttggcgtaag gcgaggcgtc aataagctcc cgcacttcct
     1501 ccggcttacc ctgcagaacg ctggcgccgt cgcgctgccg gtctttcccc agcagataat
     1561 ccaccggacc gccgccaccg ccgcggcctc tgggatgaaa cttaacgatc atcgtctgcc
     1621 cccttttcca gcacggcgtg ccgcagccgc tcgagtccgg catcaatggc catcagcacg
     1681 gcgacaacct gcacgcgatc gtgcccagta ccgccaccgg cgttaacctg ccgggcaatc
     1741 tggttgaggt tgttacccat gccggcaagc tgacgaagca gcgccggcga gatggatgga
     1801 agtttgccag cacgcgaggg cttttcgtcc aggcatatct ggcgcatcca ggccgcgagt
     1861 tgtttaccct cacagcgctc cagaagtcgg cggtgctcgt cttctgtcac ccacatagtg
     1921 agcattttgt tgcgcttttc tgccaaacca tcctccggat aacaccggtt cgggcgggaa
     1981 attttttcct gtccggagcg gtgttgggca agcccgcagg gcgcggcagt acttttcagc
     2041 gggtttcggg gcgcagccct gaaccagtca cgtagcgcta gcggagtgta tactggctta
     2101 acatggtaaa tcggagtggg gattcaggaa agtgcttcat gtggcaggga gaaaatgctg
     2161 ctgtggggcg taagtgcgaa atgcagatcc cggtgaaatg acgcctcctc gctcactcgg
     2221 tcgctgcgct cctgccgtga gactgcggcg ggcgttgcca gcttacgaat ggctggataa
     2281 agagactaag cggaaatcaa tcagaaaggg cggaaaaagg atgcggcgtt gccgtttttc
     2341 cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga
     2401 aacccgacag gactataaag atcccaggcg tttccccctg gtagctccct cgtgcgctct
     2461 cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg tttatctaat
     2521 tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga
     2581 accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgacttg agtccaaccc
     2641 ggaaagacac gacaaaacgc cactggcagc agccactggt aactgattta gaggagttag
     2701 tcttgaagtt atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgtgctc
     2761 ctccaagcca gttacctcgg ttcaaagagt tgctgctctg agaaccttcg aaaaaaccgc
     2821 cttgctgggc ggttttttcg tttacagtgc taagagatta cgattctcca tgaatgatct
     2881 caagaagatc cgtttaaaaa agcgggtttt atggggggat aaagttcgtc tagcccagtc
     2941 actttggatt acagctaggt ttcgtaagcc atgcaagaat gcctgtaact aacaaaatag
     3001 gccctgcaac gtacgtaaca tagactccaa catacgacac tgtgaaacat atgagtacgt
     3061 atatagacag tgttgtccaa aaaatgactg ttgttttaca gtgaatccac tctttcattt
     3121 ttcaataaac cccacaccgt ctggagatct tattatcgtt ttgccaggat tagctttcgc
     3181 ccaaaccaaa gcatcttgaa aagaagagaa tttttttttt gaatttttag tttttcggcg
     3241 atcttctcga aaatattcgc tgttattctc atcaattatc cgatcaaaat cagcaccagc
     3301 tccaaaggta cgctcaaaat cccccatttt ctgcccccat taaatttaat aaaaaaacag
     3361 atttaaaata tccaaaagac actcttattt accataacat cccttgttcg caccgccgca
     3421 gacagcctca gcaccgttct gcgccatgaa ccgcaaaaga gcacctctta ccacgtaaaa
     3481 acgccgataa atgccccccg taagttctta cggtatgcat agttatgcac ggaaatgaac
     3541 tgacactctg ttcaatggat tttcatgctg taagcagtat tttagtcttc tatgccccct
     3601 cgaaacacac ctgttcttgt gtagatcttt tcgacccaag atggtctttt atccgagttc
     3661 atgcagccgt acaggaacac acctgttttg ggtttatagc tccccccctc ctgtggctgg
     3721 ttatctacaa atgaataatc aatcttttca cctttgactg aagcacgccc cccaacatga
     3781 actaaagacc agtgctcatg catcacatgg ctttcaatca atccaaatga agaaaaaccc
     3841 catgaatcaa aaaagacctt gagtccgtca gacgcaccaa caggagtaat gattgcaaat
     3901 tcaacctgct caccagtacc ttcaaggtaa gaaggtttct cgacgttaat tacagatgcc
     3961 cccttcacct gataaccttt acaggtaaga tataagtccc tcccggctga cggttttg
//
