LOCUS       NZ_CP091714             3374 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli strain KFu014 plasmid pKFu014_2, complete sequence.
ACCESSION   NZ_CP091714
VERSION     NZ_CP091714.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25236397
            Assembly: GCF_022494855.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Gomi,R.
  TITLE     Genome sequencing of blaCTX-M-carrying Escherichia coli isolates
            recovered from river water in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Gomi,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2022) Department of Environmental Engineering,
            Kyoto University, Kyotodaigakukatsura, Nishikyo-Ku, Kyoto-City
            615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022494855.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 03:23:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,722
            Genes (coding)                    :: 4,584
            CDSs (with protein)               :: 4,584
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 56 of 138
            Pseudo Genes (incomplete)         :: 88 of 138
            Pseudo Genes (internal stop)      :: 31 of 138
            Pseudo Genes (multiple problems)  :: 29 of 138
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091714.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="KFu014"
                     /isolation_source="river water"
                     /db_xref="taxon:562"
                     /plasmid="pKFu014_2"
                     /geo_loc_name="Japan"
                     /lat_lon="34.6 N 135.7 E"
                     /collection_date="2011"
                     /collected_by="Kyoto University"
     gene            complement(320..643)
                     /locus_tag="L6L57_RS24070"
                     /old_locus_tag="L6L57_24070"
     CDS             complement(320..643)
                     /locus_tag="L6L57_RS24070"
                     /old_locus_tag="L6L57_24070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001696562.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            707..898
                     /locus_tag="L6L57_RS24075"
                     /old_locus_tag="L6L57_24075"
     CDS             707..898
                     /locus_tag="L6L57_RS24075"
                     /old_locus_tag="L6L57_24075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165979.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2216..2449)
                     /locus_tag="L6L57_RS24080"
                     /old_locus_tag="L6L57_24080"
     CDS             complement(2216..2449)
                     /locus_tag="L6L57_RS24080"
                     /old_locus_tag="L6L57_24080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2456..3016)
                     /locus_tag="L6L57_RS24085"
                     /old_locus_tag="L6L57_24085"
     CDS             complement(2456..3016)
                     /locus_tag="L6L57_RS24085"
                     /old_locus_tag="L6L57_24085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032329292.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISGHTTSVKQQLDTLSATVSTQFSTTEAELSRQQKKLLWQVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     Typing          complement(980..1060)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.805"
                     /coverage="93.0"
                     /evalue="8.68e-18"
     Typing          complement(2..330)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.636"
                     /coverage="83.0"
                     /evalue="3.27e-62"
     Typing          complement(2134..3374)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.885"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgggatgaca aaattcagtt cgagccgccc cttatcctgg tgttccaccc agagaatgct
       61 gtactggtct ttttcgagac cgggcataag aacacgctca aagctcgtca tcactctttc
      121 gcgttcaccc ggcggtaact ccttctccgc gaacgacaga acgcctgacg tgtatttctt
      181 cgaaaatggc gtggaatcaa tgagttcccg cacttcttcc gggttacccc ggagcaccgt
      241 tgcgccttcg cggttacgct cccgccccag caggtaatcg accgggccac ttccaccgcc
      301 tttccccctg gcatgaaatt taactatcat cccgtacccc ctgttccctg acagcctgcc
      361 gcaactggcg caattcaccc tcgatggcca tcagtgcggc caccacatga acccggtcaa
      421 tcgaagacca ctggccgctg ttcaccttcc gggctgtctg gttcaggttg ttcccgatgg
      481 cagccagatg ccgcagcaat ggcggtgcca gtgtcggcag ttttcctgtg cgtgcaaccg
      541 gctcccccag acagacccgc cgcatccatt ccgccagtcg tttaccctcg cagcgttcca
      601 gtaaccgggc gtgctcctca tcggtaaccc gtatcgtgag catcttctcg cgtttcatcg
      661 gtatcattcc ccctaacaaa tcccccatac aaggagcctt aagaccatga ccaaacagga
      721 aaaaacagcc cttaacatgg ctcgctttat cagaagccag acgctaaccc tgctggaaaa
      781 actcaacgat ctggacgcgg acgaacaggc agacatctgc gaatcacttc acgaccacgc
      841 cgacgaactc taccgcagtt gcctcgcacg cttcggcgat gacggtgaaa gcaactgaaa
      901 catgcagctc ccggagacgt tcacagcttg cctgtgagcg gatgccggga gcagacaagc
      961 ccgccagggc gcgtcagtgc gggtgtcggg gcgcagccct gacccagttc acagagcgat
     1021 agcgatgtgt atactggctt acctcatttt gcatcggtgc agagtggatg ggaagtgcac
     1081 catgtcagga gtaaaaagcc gcacagatgc gtaaaaacag cgcgcccgat aaacaacaca
     1141 cacgcggaca ctggcacagg cgcgtaagtg cacaatgctt gcctctgtga ctcttccgct
     1201 tcctcgccca ctcgctcact acgttcggtc gctggggctg cggcgagcgg tgggatcact
     1261 ctcgttgtga tgcagatccg tttttttttt tgcactggag ttatcaggag tagcggtgca
     1321 catgggatcg tgcgatatgc tggatggtgg aaggtcggag ggaacggcaa caactcaccc
     1381 ctcccagtga agggacgggt agtttgccgc tctcgatatg ccggacggag ccaaggccat
     1441 cgatcacggg gcgcgtcagt ggaagaaccc gcgcctgcgt gacgtatgcg tcttcgccgc
     1501 accatctcac ccgtcacgcc accacatacc gggaactgcc gccaaggtct aactcaacca
     1561 atctagccgc gctacatacg gtttttgtgg caactcatca ggtgtccgct gtcgatgaaa
     1621 ctctctgcgc cggtagcgga actctcacgc gtgcggtcgc cgtcgtcaca gctcccccaa
     1681 ccgcaaacga ttctcgcaaa tacgcaagta tctgcaaaaa caggtttttt tcggggttcc
     1741 tgggagactg tactgttgaa aaattcttga agaatacaga aggctcatgc aaactgaatg
     1801 aactggtttc tgttgtggat ctcaacagtt tccacaggaa cattgaggag gtcagagacc
     1861 tcaatgaacc cgaaacagct cgccttcctg gcgagctgtt ttcgttctac gcccgccgac
     1921 gaaagatcgc aagtgcaata acgagatttt tcacgtcttt acgctcaaaa agtaaaaaaa
     1981 ccatgttttc ccctggctga atccccaaaa acgcgtgaaa tgcgttttca ggatgttgca
     2041 tagctatgca caaatacggc acacgatttg tttcgctcgc ccccagccgt cgaatgggtc
     2101 aaaatggcaa ggtttcgtca cttccccggt cacatctcca ttgatggccc actccgaacc
     2161 cgcgccgcgc gttcctgtac ctcatgtctg taccgggcac gctcctgttc atcccttagc
     2221 tgttgttcgc gataaataac cgactcaagc gatccacttg cccggctaat ctctgcacct
     2281 gctgactcaa tatcacgacg cgctcgttca gtatcgcgtt gttccgttgc gtaacctcgt
     2341 gcatcttctg caattccgcg aaggcgctct cccactcgtt cagccgccgc atatagtcct
     2401 gttgcaaatg ctctaaggcg ttcagcaaat gcccttccag ttccgtcata ctcgtttact
     2461 ccctgaccag cctcacggcg ttcttgtcgc ccaccgtcca ggtgttttcc cctttcaccc
     2521 cgtccggcag caccagaaat ttcccgttct cgccattgac gaacgtcacc ccccaggttt
     2581 ttgcctcgag tttcgccagc gtgttttcct gctctctgat agtcaggata tttttcgcaa
     2641 tcttgctttc ctgccactcc atcagccccc agcaccccag gaaaatgccg cccgtgacgc
     2701 tgagcgcggt cagcgccgga aacagtatcc gccctttgat cacctgccag agcagttttt
     2761 tctgctgccg ggacagttcg gcctctgtgg tgctgaactg cgtgctcacg gtggcgctca
     2821 gtgtatccag ttgctgtttc acggatgttg tatgtccgct gatagcgtct ctgattttct
     2881 gcctgtttaa gctcagtgcc ttgtctacag actcttcgag cttcctgaac tcgctgttca
     2941 gcatgttctc ggtagaagcg gctcgctctt tcagtttctt ctcgaagtct gttcccattt
     3001 gtaaaagatt gctcatacag cgcccccttt agcctgagat tccgcccacc ctccgggtcg
     3061 gcaatactga tactgcttct ggttgtcctc accacctcaa aacctgccgc tgtgagcgcc
     3121 tcagtgacat cctgacgcgt ttttattgct ccggcatggt aaagcgcctc aagaccccgt
     3181 gtaatcgctt ctgcggcctc ctgtttcgct tttggcagat tatttggcgt gacaagcgtc
     3241 cgtctgttct ccggtgcgtt cgggtcacgc agcccgtaat gatgattaac cagcgtctgc
     3301 caggcattta tcctcggcct gtccgcacgg tcgtagtacg gctggaggcg cttaccgctc
     3361 tgtagctcca tgtt
//
