LOCUS       NZ_CP091711             2089 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli strain KGu016 plasmid pKGu016_7, complete sequence.
ACCESSION   NZ_CP091711
VERSION     NZ_CP091711.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25236402
            Assembly: GCF_022494825.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Gomi,R.
  TITLE     Genome sequencing of blaCTX-M-carrying Escherichia coli isolates
            recovered from river water in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Gomi,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2022) Department of Environmental Engineering,
            Kyoto University, Kyotodaigakukatsura, Nishikyo-Ku, Kyoto-City
            615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022494825.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 04:03:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,248
            CDSs (total)                      :: 5,124
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 211
            CDSs (without protein)            :: 211
            Pseudo Genes (ambiguous residues) :: 0 of 211
            Pseudo Genes (frameshifted)       :: 80 of 211
            Pseudo Genes (incomplete)         :: 151 of 211
            Pseudo Genes (internal stop)      :: 43 of 211
            Pseudo Genes (multiple problems)  :: 54 of 211
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091711.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="KGu016"
                     /isolation_source="river water"
                     /db_xref="taxon:562"
                     /plasmid="pKGu016_7"
                     /geo_loc_name="Japan"
                     /lat_lon="34.6 N 135.8 E"
                     /collection_date="2011"
                     /collected_by="Kyoto University"
     gene            join(1911..2089,1..88)
                     /locus_tag="L6L59_RS26130"
                     /old_locus_tag="L6L59_26130"
     CDS             join(1911..2089,1..88)
                     /locus_tag="L6L59_RS26130"
                     /old_locus_tag="L6L59_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            942..1907
                     /locus_tag="L6L59_RS26135"
                     /old_locus_tag="L6L59_26135"
     CDS             942..1907
                     /locus_tag="L6L59_RS26135"
                     /old_locus_tag="L6L59_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          1336..1567
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 tcagaaaaca aacgagaggg taaaaactct ttttgtgaaa atagatttaa acggtatcaa
       61 gtttggattt ttaagaacgc attcttagtt ctggaaaaga gccagcggca ggctgaggtg
      121 ataggtacga gattgcatgc aatctctagt gctctgtcta tcctgcatta tcctcagcat
      181 tatcctcagc cttgccaact cgacaccaat gcaggataga caatccgatg tcaaatgtta
      241 acactctgcg agtggtacat tttccccgga ttatcgtcct gagcctgccg ctggctctct
      301 ttctaccgcc tcgctttgct cgttgctcaa cgcctcacag acacggatta aaatccgcat
      361 ccgttcaccg ttttttaaag tccgttaaaa gcatgatgcc atctccgaga gttaatctcg
      421 tcaaatgcta aatcgtgggg gtcccctttg gggttccgat ttagtgattg acgacaccac
      481 cgattaaaaa acttatgcgg ggtggatggt ttcacgaagt gaggccatcc acctgtaaga
      541 cagggttttg tttttattcc ctgttttggt gatcgggtgt gtggaaaagg ttggggtaag
      601 ccgttcgggg gtgcttgttt tggggggtta aagttgtggt tattttttgc gcaattctcg
      661 cgcgtgatcc ttgtatttat acttaaggga taaatggcgg atatgaaata gtggtttagc
      721 ccagtaatga cgaggctttg agtgggtttt gacaggtcaa agaaaatgga gcagaattga
      781 ggcgttttta atcggcgttg gggagtgcgt caacactccc caacatttcg aatgtgtcac
      841 ctcagcggca aactctggtg acatgtactg gctcgcaatg cacaggtacg tgatgaatat
      901 accacatcaa atcacagcct gcccagatcg gagcaggctt aatgtcagaa gataaattcc
      961 tttcggacta cagcccccgt gatgcagttt gggataccca gcgcacgctt accgattctg
     1021 tcgggggtat ctaccagact gctgctgaat tcgagcgcta tgcactccgt atggcctcct
     1081 gtagcggttt gttacgtttt ggttggtcta ccatcatgga aaccggagaa acgcgcctac
     1141 ggcttcgtag tgcgcaattt tgccgtgtcc gtcattgccc tgtctgccag tggagaagaa
     1201 ccctcatgtg gcaagcccgt ttttatcagg ctctaccgaa aatcgttgtg gattacccgt
     1261 cttcccgatg gttgtttctg acgttaactg tcaggaactg cgagataggt gaacttggaa
     1321 cagtccttac agcaatgaat gcggcgttta agcgaatgga aaagcgaaag gagctatcac
     1381 ctgttcaggg gtggatcagg gctacggagg tgacgcgagg taaggatggc agcgcacatc
     1441 cgcattttca ctgtctgctg atggtgcaac cttcttggtt taaagggaag aactacgtta
     1501 agcacgaacg ttgggtagaa ctctggcgcg attgcttgcg ggtgaactat gagccgaata
     1561 tcgatattcg ggcagtaaaa actaagacag gtgaggttgt ggccaacgtt gccgagcaac
     1621 tgcaaagcgc ggttgctgaa acgctgaaat actccgttaa accggaagat atggcaaacg
     1681 atcctgagtg gtttcttgag ctgacgcggc agcttcacaa gcgccgtttt atctcgaccg
     1741 gtggggcgct aaaaaacgtc ctccagttgg atcgagaaac caatgaggat cttgtcattg
     1801 ccgacgatgt aggggatggc actgatgacg ggaagcggac ggcgtttgtc tgggattcag
     1861 gtaaacggcg ttacaaacgc gcccctgaga aggataaatc ggattaacgt atgaatatta
     1921 atattgaata cctgaatgga aataagacta ttggtttatt ttttttaaga agtgaagcgg
     1981 tgattcctga caggtttaaa aaccttattt tgcttattga tggattaagt tttggcacat
     2041 ttggttttca tccgcacgaa ggttttgagg atgaattaat tttatatat
//
