LOCUS       NZ_CP091702             2514 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli strain KFu019 plasmid pKFu019_4, complete sequence.
ACCESSION   NZ_CP091702
VERSION     NZ_CP091702.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25236398
            Assembly: GCF_022494785.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Gomi,R.
  TITLE     Genome sequencing of blaCTX-M-carrying Escherichia coli isolates
            recovered from river water in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Gomi,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2022) Department of Environmental Engineering,
            Kyoto University, Kyotodaigakukatsura, Nishikyo-Ku, Kyoto-City
            615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022494785.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 03:36:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,000
            CDSs (total)                      :: 4,877
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 71 of 227
            Pseudo Genes (incomplete)         :: 141 of 227
            Pseudo Genes (internal stop)      :: 49 of 227
            Pseudo Genes (multiple problems)  :: 32 of 227
            Pseudo Genes (short protein)      :: 1 of 227
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091702.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="KFu019"
                     /isolation_source="river water"
                     /db_xref="taxon:562"
                     /plasmid="pKFu019_4"
                     /geo_loc_name="Japan"
                     /lat_lon="34.6 N 135.7 E"
                     /collection_date="2011"
                     /collected_by="Kyoto University"
     gene            join(1685..2514,1..139)
                     /gene="mobV"
                     /locus_tag="L6L55_RS24885"
                     /old_locus_tag="L6L55_24880"
     CDS             join(1685..2514,1..139)
                     /gene="mobV"
                     /locus_tag="L6L55_RS24885"
                     /old_locus_tag="L6L55_24880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            complement(455..937)
                     /locus_tag="L6L55_RS24890"
                     /old_locus_tag="L6L55_24885"
     CDS             complement(455..937)
                     /locus_tag="L6L55_RS24890"
                     /old_locus_tag="L6L55_24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202974839.1"
                     /translation="MNTKKISKLERHTYMDESGEIKQDSKIETFSIPAEPPYVKLYIAD
                     IAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVD
                     KQIFLRAGRGELEANPFLFARGDWPSIYERRKKIELRISYDSNGRTVQTTVTDDER"
                     /name="hypothetical protein"
     Typing          1677..2512
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.624"
                     /coverage="83.0"
                     /evalue="0.0"
ORIGIN
        1 cagagagcag atgaccgaaa tcctgatgat ggctcagcgg tttaagcagc aaaaccagga
       61 aaaggaaaaa cagcagcgta ttgaacgtga aaaacagcga cagacgcgat cgcgtggcat
      121 gggtggtatg gagcgttagt tttgatgcga tgcaggatag ggctaacgcc gcaaagtgac
      181 gtcatttgcg gcattccaga gatcaggttc tgccgccctg tataacgctg agtccagcta
      241 atccatcaga tggtaattgt attttttcaa aatagaaaaa gcggtgcttt tttgttttgg
      301 tgacaatccc ttagttgaaa attcattacc gcttgttctg cgatatatgc tgtatgtact
      361 acttaaaaaa gtacgctccc aaacagtcaa aacctcattc ttttttagag agtcatgcag
      421 cattaatcta tctacttttt tgaatagctt atcttcagcg ttcatcatcc gttaccgttg
      481 tttgtactgt ccttccattg gaatcataac tgattcttaa ctctattttt tttcttcttt
      541 cgtaaattga aggccagtct ccccgagcaa aaagaaacgg attggcttca agctctcccc
      601 gccctgcccg gagaaagatt tgtttatcaa caagggcagc gattatgttt ttaaacgtct
      661 gatattcaat atgaacccta ggttgtatgg tcttctcaaa tctagttttt gatacagata
      721 caaagccatc ccatccaatc agcattgcca actgcaaaag cacatcattt tgagattttg
      781 acaaaccatg aatcatcgca atgtctgcga tgtaaagctt gacgtagggc ggttcagccg
      841 ggatagaaaa agtctcgatc ttagagtctt gtttaatctc tccgctctcg tccatgtatg
      901 tatgccgttc aagcttagaa atcttttttg tattcactct agccccatgt atcagcatgt
      961 ttttagtact cacatttaag tactaaagat gccggtagtc aagtacctta cctaaagtac
     1021 gattagtact tgaggtgagt acgattagta cttgaggtaa gtacgattag tacttgaggt
     1081 aagtacgatt agtacttgag gtgagtacga ttcttctctc tagcccttgg tgtgcgcggg
     1141 ttcccgtatc tatatctata tattaagggg gaaaataaag gctgaaaaac gccattttta
     1201 aacatcgaaa aacgagcttt tttgagtctg gagcgtacgg atttctatgt ccggggagtg
     1261 gaaattccgc ttcctcgaac ggacagactc gcggcgatgc cgctccctaa tccgttcttg
     1321 cggcgagcgg ttcctgctct ttcaaaaacg atattcattg cctgatttcg tcaagggcga
     1381 gcggttcctg ctctttcaaa aacgatattc attgcctgat ttcgtcaagg gcgagcgtag
     1441 cgagtgccat ttacccttga cgactcaccg gataaaacag gcttggcagt ggtgggaggt
     1501 gacgcggagc gacgccttcc acctgccaga ccgccgttga ttttgacctt tggaagcgca
     1561 gcttcccgaa cgattttgcg aagcaaagtc tagtgagtac actatcatct tgatagtggc
     1621 ccgccggagg tatcgcaatg cgctcccccg cctggctgaa tgaatgccgg aatcaggggg
     1681 gaacatggcg aactacgcga taatgcgctg caaaaagctg acgggaatgg gaagcgtggc
     1741 cagcgcgttg cagcactgct accgagagcg tgagacgccg aacgctaacg ctgaacggac
     1801 accagaaaac tactgctctg tgtccaaatc aacggatcag gcaatggggc gtgtgcggga
     1861 gctgctgccg gaaaaacgcc gtaaagatgc ggtactggcc gttgaatacg ttatgacagc
     1921 aagccctgag tggtggaaag aggccacacc acggcagcag gcggagtttt tcgcccgctc
     1981 tgagcaatgg ctggagaaga aatacgggaa ggatcgtgtt gtggcggcgg tggttcaccg
     2041 ggacgaggcc accccgcacc tgtctgcgtt cgtggtgcca ttgacgcagg atggacggct
     2101 gtccgcgaaa gagtttattg gcgggcggtc aaagatgcgt gaagatcaga gcacctacgc
     2161 tgaatcggta aaaaaactgg gtcttgagcg cggcatcgag ggaagccgtg ccacgcatca
     2221 gacagttcag cactactacg aatcgataaa ccgtggcacc cgtagccagg tatcgatctc
     2281 accggaggcg ctggagccgc gtgtactgcg gaagggtatt ttcaccaaag acgtggaaga
     2341 tcaggcagcc atagccaaac gtctaagcca cgcagtgaac gatggctttg caggaaccat
     2401 cgctatggcc tctcagagcg cccagaacgc aaaaagagcg cgtgagctac agaagactat
     2461 ggatgcgcag cagaaacgcc tacagagcgt tacagagcct tttaaggggc tttc
//
