LOCUS       NZ_CP091697             2113 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli strain KMi006 plasmid pKMi006_6, complete sequence.
ACCESSION   NZ_CP091697
VERSION     NZ_CP091697.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25236407
            Assembly: GCF_022494765.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Gomi,R.
  TITLE     Genome sequencing of blaCTX-M-carrying Escherichia coli isolates
            recovered from river water in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Gomi,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2022) Department of Environmental Engineering,
            Kyoto University, Kyotodaigakukatsura, Nishikyo-Ku, Kyoto-City
            615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022494765.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 03:35:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,041
            CDSs (total)                      :: 4,921
            Genes (coding)                    :: 4,646
            CDSs (with protein)               :: 4,646
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 275
            CDSs (without protein)            :: 275
            Pseudo Genes (ambiguous residues) :: 0 of 275
            Pseudo Genes (frameshifted)       :: 93 of 275
            Pseudo Genes (incomplete)         :: 191 of 275
            Pseudo Genes (internal stop)      :: 52 of 275
            Pseudo Genes (multiple problems)  :: 55 of 275
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091697.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="KMi006"
                     /isolation_source="river water"
                     /db_xref="taxon:562"
                     /plasmid="pKMi006_6"
                     /geo_loc_name="Japan"
                     /lat_lon="34.6 N 135.7 E"
                     /collection_date="2011"
                     /collected_by="Kyoto University"
     gene            join(1559..2113,1..411)
                     /locus_tag="L6L65_RS25025"
                     /old_locus_tag="L6L65_25030"
     CDS             join(1559..2113,1..411)
                     /locus_tag="L6L65_RS25025"
                     /old_locus_tag="L6L65_25030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            415..681
                     /locus_tag="L6L65_RS25030"
                     /old_locus_tag="L6L65_25035"
     CDS             415..681
                     /locus_tag="L6L65_RS25030"
                     /old_locus_tag="L6L65_25035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          1953..2113
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.030000000000001e-79"
ORIGIN
        1 gttaagcacg aacgttgggt agaactctgg cgcgattgct tgcgggtgaa ctatgagccg
       61 aatatcgata ttcgggcagt aaaaactaag acaggtgagg ttgtggccaa cgttgccgag
      121 caactgcaaa gcgcggttgc tgaaacgctg aaatactccg ttaaaccgga agatatggca
      181 aacgatcctg agtggtttct tgagctgacg cggcagcttc acaagcgccg ttttatctcg
      241 accggtgggg cgctaaaaaa cgtcctccag ttggatcgag aaaccaatga ggatcttgtc
      301 attgccgacg atgtagggga tggcactgat gacgggaagc ggacggcgtt tgtctgggat
      361 tcaggtaaac ggcgttacaa acgcgcccct gagaaggata aatcggatta acgtatgaat
      421 attaatattg aatacctgaa tggaaataag actattggtt tatttttttt aagaagtgaa
      481 gcggtgattc ctgacaggtt taaaaacctt attttgctta ttgatggatt aagttttggc
      541 acatttggtt ttcatccgca cgaaggtttt gaggatgaat taattttata tattcagaaa
      601 acaaacgaga gggtaaaaac tctttttgtg aaaatagatt taaacggtat caagtttgga
      661 tttttaagaa cgcattctta gttctggaaa agagccagcg gcaggctgag gtgataggta
      721 cgagattgca tgcaatctct agtgctctgt ctatcctgca ttatcctcag cattatcctc
      781 agcattatcc tcagcattat cctcagcctt gccaactcga caccaatgca ggatagacaa
      841 tccgatgtca aatgttaaca ctctgcgagt ggtacatttt ccccggatta tcgtcctgag
      901 cctgccgctg gctctctttc taccgcctcg ctttgctcgt tgctcaacgc ctcacagaca
      961 cggattaaaa tccgcatccg ttcaccgttt tttaaagtcc gttaaaagca tgatgccatc
     1021 tccgagagtt aatctcgtca aatgctaaat cgtgggggtc ccctttgggg ttccgattta
     1081 gtgattgacg acaccaccga ttaaaaaact tatgcggggt ggatggtttc acgaagtgag
     1141 gccatccacc tgtaagacag ggttttgttt ttattccctg ttttggtgat cgggtgtgtg
     1201 gaaaaggttg gggtaagccg ttcgggggtg cttgttttgg ggggttaaaa ttgtggttat
     1261 tttttgcgca attctcgcgc gtgatccttg tatttatact taagggataa atggcggata
     1321 tgaaatagtg gtttagccca gtaatgacga ggctttgagt gggttttgac aggtcaaaga
     1381 aaatggagca gaattgaggc gtttttaatc ggcgttgggg agtgcgtcaa cactccccaa
     1441 catttcgaat gtgtcacctc agcggcaaac tctggtgaca tgtactggct cgcaatgcac
     1501 aggtacgtga tgaatatacc acatcaaatc acagcctgcc cagatcggag caggcttaat
     1561 gtcagaagat aaattccttt cggactacag cccccgtgat gcagtttggg atacccagcg
     1621 cacgcttacc gattctgtcg ggggtatcta ccagactgct gctgaattcg agcgctatgc
     1681 actccgtatg gcctcctgta gcggtttgtt acgttttggt tggtctacca tcatggaaac
     1741 cggagaaacg cgcctacggc ttcgtagtgc gcaattttgc cgtgtccgtc attgccctgt
     1801 ctgccagtgg agaagaaccc tcatgtggca agcccgtttt tatcaggctc taccgaaaat
     1861 cgttgtggat tacccgtctt cccgatggtt gtttctgacg ttaactgtca ggaactgcga
     1921 gataggtgaa cttggaacag tccttacagc aatgaatgcg gcgtttaagc gaatggaaaa
     1981 gcgaaaggag ctatcacctg ttcaggggtg gatcagggct acggaggtga cgcgaggtaa
     2041 ggatggcagc gcacatccgc attttcactg tctgctgatg gtgcaacctt cttggtttaa
     2101 agggaagaac tac
//
