LOCUS       NZ_CP091674            18520 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli strain KKo009 plasmid pKKo009_3, complete sequence.
ACCESSION   NZ_CP091674
VERSION     NZ_CP091674.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25236406
            Assembly: GCF_022494705.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18520)
  AUTHORS   Gomi,R.
  TITLE     Genome sequencing of blaCTX-M-carrying Escherichia coli isolates
            recovered from river water in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18520)
  AUTHORS   Gomi,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2022) Department of Environmental Engineering,
            Kyoto University, Kyotodaigakukatsura, Nishikyo-Ku, Kyoto-City
            615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022494705.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 03:58:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,067
            CDSs (total)                      :: 4,947
            Genes (coding)                    :: 4,740
            CDSs (with protein)               :: 4,740
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 92 of 207
            Pseudo Genes (incomplete)         :: 133 of 207
            Pseudo Genes (internal stop)      :: 42 of 207
            Pseudo Genes (multiple problems)  :: 52 of 207
            Pseudo Genes (short protein)      :: 1 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091674.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..18520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="KKo009"
                     /isolation_source="river water"
                     /db_xref="taxon:562"
                     /plasmid="pKKo009_3"
                     /geo_loc_name="Japan"
                     /lat_lon="34.6 N 135.8 E"
                     /collection_date="2012"
                     /collected_by="Kyoto University"
     gene            complement(join(18316..18520,1..1226))
                     /locus_tag="L6L68_RS24565"
                     /old_locus_tag="L6L68_24560"
                     /pseudo=""
     CDS             complement(join(18316..18520,1..1226))
                     /locus_tag="L6L68_RS24565"
                     /old_locus_tag="L6L68_24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041207141.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS50R family transposase"
                     /name="IS4-like element IS50R family transposase"
     gene            1346..1534
                     /locus_tag="L6L68_RS24570"
                     /old_locus_tag="L6L68_24565"
     CDS             1346..1534
                     /locus_tag="L6L68_RS24570"
                     /old_locus_tag="L6L68_24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000957857.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVTTSIL
                     KRRGDTAALTLLQDTGV"
                     /name="hypothetical protein"
     gene            1544..2743
                     /locus_tag="L6L68_RS24575"
                     /old_locus_tag="L6L68_24570"
     CDS             1544..2743
                     /locus_tag="L6L68_RS24575"
                     /old_locus_tag="L6L68_24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="WP_000948429.1"
                     /translation="MLSGFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACGT
                     RILGVKEFGCDNPDCQHVKYLTNSCGSRACPSCGKKATDLWTATQLNRLPDCDWVHLVF
                     TLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHPHVH
                     VSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGMAMPESLSHITTESQW
                     RSLVLKSGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLD
                     HKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKP
                     EPVAKVCYAQMVKQFLSRDPFECVLCGCRMVYRRAIAGLNVSGLKKNARDISLLRYMPA
                     "
                     /name="IS91 family transposase"
     gene            <2909..3001
                     /locus_tag="L6L68_RS24580"
                     /old_locus_tag="L6L68_24575"
                     /pseudo=""
     CDS             <2909..3001
                     /locus_tag="L6L68_RS24580"
                     /old_locus_tag="L6L68_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /name="IS6 family transposase"
     gene            complement(3240..3521)
                     /locus_tag="L6L68_RS24585"
                     /old_locus_tag="L6L68_24580"
     CDS             complement(3240..3521)
                     /locus_tag="L6L68_RS24585"
                     /old_locus_tag="L6L68_24580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000220561.1"
                     /translation="MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLH
                     GHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGRRESSEVYQIADMR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3511..3762)
                     /locus_tag="L6L68_RS24590"
                     /old_locus_tag="L6L68_24585"
     CDS             complement(3511..3762)
                     /locus_tag="L6L68_RS24590"
                     /old_locus_tag="L6L68_24585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_000121743.1"
                     /translation="MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCV
                     PPKLYAYYRELAEDAELNAVADERMKNPEIVKVNLDDL"
                     /name="plasmid stabilization protein"
     gene            4997..5836
                     /locus_tag="L6L68_RS24595"
                     /old_locus_tag="L6L68_24590"
     CDS             4997..5836
                     /locus_tag="L6L68_RS24595"
                     /old_locus_tag="L6L68_24590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722379.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_024129959.1"
                     /translation="MNLLSVTKKTKIRHRNEINSTFSSLPLSARRILFMAMAQIDSREE
                     LSEGATFRITAREYAFIADIDVTGAYRQLKEGAEELQASVIRIPRNQLLTAQGNQSSGH
                     TKKRGKLPSDAVRLMNLTAFCDYVESEGYIDIAFSHVIEPYITMLKDSYTTQVLISSVR
                     LADQNSNMLYQFIRKQYSSGKKTFFDIEVDVLKDELELFRIDNGEKVYLYQNFKDFNKV
                     FLQKNIEKINQYTEINHLEVKIVKRVARRASKLRFSYKIDKESEGLDTRIPYGFRGA"
                     /name="replication initiation protein"
     gene            5857..6087
                     /locus_tag="L6L68_RS24600"
                     /old_locus_tag="L6L68_24595"
     CDS             5857..6087
                     /locus_tag="L6L68_RS24600"
                     /old_locus_tag="L6L68_24595"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063090927.1"
                     /translation="MEQIKLHKQGGAAVIIIPASMIENTGWEDGTLLEVLEINETLHIR
                     RKTESGALATKACPSLFSSTMRHVNYDKIIR"
                     /name="hypothetical protein"
     gene            complement(6098..6795)
                     /locus_tag="L6L68_RS24605"
                     /old_locus_tag="L6L68_24600"
     CDS             complement(join(6098..6546,6546..6795))
                     /locus_tag="L6L68_RS24605"
                     /old_locus_tag="L6L68_24600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223367350.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            6895..7476
                     /locus_tag="L6L68_RS24610"
                     /old_locus_tag="L6L68_24605"
     CDS             6895..7476
                     /locus_tag="L6L68_RS24610"
                     /old_locus_tag="L6L68_24605"
                     /inference="COORDINATES: protein motif:HMM:NF013235.4"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_235638730.1"
                     /translation="MSFSARRVFFICLYYYFENKLESDKSLLIRADKYAEFVGVERSVA
                     YKQMKDAADFFSRNKNLITHCDYISNEGLLRVMFSSKTINYITAIDSRKYQVTELPLKS
                     ALSLQGRYSWPLYQLIKFSHLKSFTIGVDELKKEIGYIQNQKFSFFKRDGINIAIDEIT
                     ERTDVKNIKCVNAERQGRRVSKVRFEIEMR"
                     /name="replication initiation protein"
     gene            7480..7926
                     /locus_tag="L6L68_RS24615"
                     /old_locus_tag="L6L68_24610"
     CDS             7480..7926
                     /locus_tag="L6L68_RS24615"
                     /old_locus_tag="L6L68_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001074384.1"
                     /translation="MAKIYQFPQGEERAKFRKEIARERKKRFAVKTGSTFVKWLGWGWF
                     YLRLLAASVLHFIFVFVLAILGAFKWAVFWLGGILCVITWYHLDHQLWTPQNLTIPVIT
                     ATWILGLLAEPLMELLNKTMPWHKLLVPEPADESTENSSEISQQ"
                     /name="hypothetical protein"
     gene            8063..8416
                     /gene="ddp1"
                     /locus_tag="L6L68_RS24620"
                     /old_locus_tag="L6L68_24615"
     CDS             8063..8416
                     /gene="ddp1"
                     /locus_tag="L6L68_RS24620"
                     /old_locus_tag="L6L68_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /protein_id="WP_024129958.1"
                     /translation="MKYLVKEFINEKYSKAVNILKDNLKEHYHVFYGVRLSEILFPTSE
                     YGTDAFFKEFELINSVILPLVIFDFIDRKPIMVIGFEEAPGIDSLIDSGMEVVLLDGLS
                     DLLLVEKLMPLFD"
                     /name="ddp1"
     gene            complement(8954..10018)
                     /locus_tag="L6L68_RS24625"
                     /old_locus_tag="L6L68_24620"
     CDS             complement(8954..10018)
                     /locus_tag="L6L68_RS24625"
                     /old_locus_tag="L6L68_24620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849600.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_012291478.1"
                     /translation="MKTISLFNNKGGVGKTTLTWNLSVSLSAKGKSVLLIDFDPQCNLS
                     IALLGETGFSDLLKKDANNPYGKTIRAFCQPFIQQDILPVVHTFPPQFPMEPGSGRLDI
                     APGDFWLNNLSDVISVGTDLIAGNAVNRFLIPSLIASKAQELNNCEYDYVLIDVPPSFN
                     TIVRSALYCSDYFLVPCTADIFSAYCIGLIGETLPKFRDDWEQGIRRTSAQNNSLISQK
                     GSPKFAGWVFNGFDKRKNPGQQNSSEIAADAAHRDKIIDSVKNDLRPKLASIKTYSAVA
                     SFTPDTPVAKVEDLNVMAPDSILQGVPLRYLSTVAPTRGVLARGAWSGPQKALMQTMSA
                     EHDVLADYIIQNFV"
                     /name="ParA family protein"
     gene            complement(10442..10672)
                     /locus_tag="L6L68_RS24630"
                     /old_locus_tag="L6L68_24625"
     CDS             complement(10442..10672)
                     /locus_tag="L6L68_RS24630"
                     /old_locus_tag="L6L68_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partition protein ParG"
                     /protein_id="WP_000051066.1"
                     /translation="MSLEKAHTSVKKMTFGENRDLERVVTAPVSSGKIKRVNVNFDEEK
                     HTRFKAACARKGTSITDVVNQLVDNWLKENE"
                     /name="plasmid partition protein ParG"
     gene            complement(10724..11344)
                     /locus_tag="L6L68_RS24635"
                     /old_locus_tag="L6L68_24630"
     CDS             complement(10724..11344)
                     /locus_tag="L6L68_RS24635"
                     /old_locus_tag="L6L68_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864791.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000864788.1"
                     /translation="MKVISFLNPKGGSGKTTAVINIATALSRSGYNIAVVDTDPQMSLT
                     NWSKAGKAAFDVFTAASEKDVYGIRKDLADYDFAIVDGAGSLSVITSAAVMVSDLVIIP
                     VTPSPLDFSAAGSVVTVLEAQAYSRKVEARFLITRKIEMATMLNVLKESIKDTGVKAFR
                     TAITQRQVYVKSILDGDSVFESSDGAAKGEIEILTKEIVRMFE"
                     /name="ParA family protein"
     gene            11639..12283
                     /locus_tag="L6L68_RS24640"
                     /old_locus_tag="L6L68_24635"
     CDS             11639..12283
                     /locus_tag="L6L68_RS24640"
                     /old_locus_tag="L6L68_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001503708.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000203199.1"
                     /translation="MTSNAPTNFVRAYLRASTAEQDASRALETIEAFARERGLIICSYY
                     IENESGSRLDRPELFRLLRDCQQNDILLIEDVDRLSRLSGEDWNTLKKMIRQKDIRVMA
                     VNVPTTWINSGMSEFDSRLFAAINDMLLDMLAAVARRDYEQRRERQKQGIEKARKDGKY
                     KGRKPNQARHDAIIRLIESGSSWTQVQKVLGCSRGTISSAIKRKSLQSSGE"
                     /name="recombinase family protein"
     gene            complement(12399..12578)
                     /locus_tag="L6L68_RS24645"
                     /old_locus_tag="L6L68_24640"
     CDS             complement(12399..12578)
                     /locus_tag="L6L68_RS24645"
                     /old_locus_tag="L6L68_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000347021.1"
                     /translation="MDFMSILGRCLLLPFPVAMFVGCLFPGIDDRFTGMFMSFIVGCLS
                     WYITKPKPQKAEVA"
                     /name="hypothetical protein"
     gene            complement(12645..12740)
                     /gene="dqlB"
                     /locus_tag="L6L68_RS25335"
     CDS             complement(12645..12740)
                     /gene="dqlB"
                     /locus_tag="L6L68_RS25335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_000609146.1"
                     /translation="MINLLIIVLRAVAALANALIAVLELIRQFID"
                     /name="dqlB"
     gene            complement(13301..13384)
                     /gene="dqlB"
                     /locus_tag="L6L68_RS25340"
     CDS             complement(13301..13384)
                     /gene="dqlB"
                     /locus_tag="L6L68_RS25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_272056275.1"
                     /translation="MMNLLIIVLRAVLAIANALIAIQKLIE"
                     /name="dqlB"
     gene            complement(13876..>14085)
                     /locus_tag="L6L68_RS24650"
                     /old_locus_tag="L6L68_24645"
                     /pseudo=""
     CDS             complement(13876..>14085)
                     /locus_tag="L6L68_RS24650"
                     /old_locus_tag="L6L68_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            14150..14854
                     /locus_tag="L6L68_RS24655"
                     /old_locus_tag="L6L68_24650"
     CDS             14150..14854
                     /locus_tag="L6L68_RS24655"
                     /old_locus_tag="L6L68_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067858.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <14912..>16297
                     /locus_tag="L6L68_RS24660"
                     /old_locus_tag="L6L68_24655"
                     /pseudo=""
     CDS             <14912..>16297
                     /locus_tag="L6L68_RS24660"
                     /old_locus_tag="L6L68_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /name="Tn3-like element Tn3 family transposase"
     gene            complement(16482..17186)
                     /locus_tag="L6L68_RS24665"
                     /old_locus_tag="L6L68_24660"
     CDS             complement(16482..17186)
                     /locus_tag="L6L68_RS24665"
                     /old_locus_tag="L6L68_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067858.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(<17236..17472)
                     /locus_tag="L6L68_RS24670"
                     /old_locus_tag="L6L68_24665"
                     /pseudo=""
     CDS             complement(<17236..17472)
                     /locus_tag="L6L68_RS24670"
                     /old_locus_tag="L6L68_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131935.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(17493..18287)
                     /locus_tag="L6L68_RS24675"
                     /old_locus_tag="L6L68_24670"
     CDS             complement(17493..18287)
                     /locus_tag="L6L68_RS24675"
                     /old_locus_tag="L6L68_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-IIa"
                     /protein_id="WP_000572405.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /name="aminoglycoside O-phosphotransferase APH(3')-IIa"
     ARG             complement(17497..18287)
                     /name="aph(3')-IIa"
                     /gene="aph(3')-IIa"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3')-IIa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          7481..7926
                     /name="IncX1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt
       61 tctacgtgtt ccgcttcctt tagcagccct tgcgccctga gtgcttgcgg cagcgtgaag
      121 ctttctctga gctgtaacag cctgaccgca acaaacgaga ggatcgagac catccgctcc
      181 agattatccg gctcctccat gcgttgcctc tcggctcctg ctccggtttt ccatgcctta
      241 tggaactcct cgatccgcca gcgatgggta taaatgtcga tgacgcgcaa ggcttgggct
      301 agcgactcga ccggttcgct ggtcagcaac aaccatttca acggggtctc acccttgggc
      361 gggttaatct cctcggccag caccgcgttg agcgtgatat tcccctgttt tagcgtgatg
      421 cgcccactgc gcaggctcaa gctcgccttg cgggctggtc gatttttacg tttaccgcgt
      481 ttatccacca cgcccttttg cggaatgctg atctgatagc cacccaactc cggttggttc
      541 ttcagatggt cgtacagata caacccagac tctacgtcct tgcgtgggtg cttggagcgc
      601 accacgaagc gctcgttatg cgccagtttg tcctgcagat aagcatgaat atcggcttcg
      661 cggtcacaga ccgcaatcac gttgctcatc atgctgccca tgcgtaaccg gctagttgcg
      721 gccgctgcca gccatttgcc actctccttt tcatccgcat cggcagggtc atccgggcgc
      781 atccaccact cctgatgcag taatcctacg gtgcggaatg tggtggcctc gagcaagaga
      841 acggagtgaa cccaccatcc gcgggattta tcctgaatag agcccagctt gccaagctct
      901 tcggcgacct ggtggcgata actcaaagag gtggtgtcct caatggccag cagttcggga
      961 aactcctgag ccaacttgac tgtttgcatg gcgccagcct ttctgatcgc ctcggcagaa
     1021 acgttgggat tgcggataaa tcggtaagcg ccttcctgca tggcttcact accctctgat
     1081 gagatggtta ttgatttacc agaatatttt gccaattggg cggcgacgtt aaccaagcgg
     1141 gcagtacggc gaggatcacc cagcgccgcc gaagagaaca cagatttagc ccagtcggcc
     1201 gcacgatgaa gagcagaagt tatcatgaac cacggcagcc tggcggctgc aaattgaaaa
     1261 ccgccccggt tcgtcctgtt gttgccgcac gccccgcaga atatgtatgc tctgaggacc
     1321 tgttttatta aggactcatc cccttatgtt gacccgcaaa agtatcgata cagttctgct
     1381 ctctgttggt gctgagaagc tctcacaacg agaatgggac tggatgaaaa tgctcaaacc
     1441 catggaccca ccacctgcga tggtgacgac ctccatcctg aagcgccgtg gcgatacagc
     1501 agcccttacc ctgctacagg ataccggggt ttaatccatc ctcatgttgt ccggttttac
     1561 tccccgccct ctgaaacgtc tgttcacagc caaccagtgc tggacgtcct tcctggatgc
     1621 gggcggtctg cgcgatatcg aggttgaagc cgtcaccaaa atgctggcct gcggcacacg
     1681 gatactgggt gtaaaagagt tcgggtgtga taacccggac tgtcagcacg taaagtacct
     1741 gaccaactca tgcggcagcc gtgcctgccc gtcctgcgga aagaaggcca cagacctgtg
     1801 gacagcaaca cagctgaatc gtcttcctga ctgcgactgg gtacatctgg tcttcaccct
     1861 gccggacacg ctgtggccgg tgttcgaaag caaccgctgg ctgctgaatg acgtgtgccg
     1921 tctggcggtg gagaatctgc tgtatgccgc ccgaaaacgg gggctggaac ccggtatctt
     1981 ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac atgtgtctgt
     2041 aacctgtgga ggtctgaata agcatggtca gtggaaaaag ctgagcttcc tgaaagacgc
     2101 gatgcgttca cggtggatgt ggaatatgcg gcagctgctt ctgaaagcgt ggtcagaggg
     2161 gatggcaatg ccggagtcgt tgtcacatat cacgacggaa tcacagtgga gaagcctggt
     2221 gctgaaatcc ggcggaaaat actggcatgt gtacatgtcg aagaagacag ccggagggcg
     2281 gaatacggca cgctacctgg gtcgttatct gaagaagcca ccaatagcgg cttcccgact
     2341 ggctcattac aacggagggg caagcctgag cttccgttac ctggaccaca aaacgggaga
     2401 aacggcgacg gaaacgctga cacagcgtga gctggtcgcg aggctgaaac agcacatccc
     2461 ggagaagttt tttaagatgg tgaggtactt cgggttcctt gccaaccgtg tgtgtggaga
     2521 gaagctgccg caggtgtacc gtgcactggg gatggataaa ccggaaccag tggcgaaagt
     2581 gtgctatgca caaatggtga aacagttcct gagtcgtgac ccgttcgaat gcgtgctgtg
     2641 tggctgccgg atggtatacc gccgggccat tgcggggctg aatgtatcag ggctgaagaa
     2701 aaacgcgcgg gatatcagtc tactgaggta tatgccggcc tgacacaggc taagtgtgcc
     2761 cgggaatcgg tgaaagtata aaaaacagcc tgttacggta taaaaatcga ccatacagag
     2821 agggaaacca ccggagtggt gggaaaagca gaaaggctca cgtggcataa ggcgtggaca
     2881 atggtatcga ctggtttttc aatttcctat acgtgcacta cgcaaaggcc aggcctcagc
     2941 attttattat ggtgatcccc tgggcgaaat gcgcctggta agcagagttt ttgaaatgta
     3001 aggcctttga ataagacaaa aggctgcctc atcgctaact ttgcaacagt gcccggaaac
     3061 aaaccagctt aattaagatc agggacatcc cccctccctg caaagcccat taccggacta
     3121 acaacacgca attagccttt cgcagttagc gcgtaagctt gcgaacggta cgccgtgacg
     3181 gtgtgtagca tctgcgcgtc tgagcggctg cagccgcgaa ttacggggcg ggtttcagtt
     3241 cagcgcatat ctgcaatctg ataaacttcg ctgctttccc ttcttccaac agcaataacg
     3301 agtaaaacga ctttctcatc aatgacttga tatacaagtc tgtagccaga tgcacgaagt
     3361 ttgattttat agcgatcagc atgaccatgt aaccgggctg cgggtacgcg tggattttcc
     3421 agccgttctg ccagtttctt tttgaattgt tcacggatgg tgtggccgag tttctgccat
     3481 tccttcagtg ctctacggtc aaaagccagt tcataggtca tccaggttaa ccttcacaat
     3541 ttccgggttt ttcatgcgct catcagcaac agcgtttaac tcagcatctt cagcgagttc
     3601 ccgatagtag gcgtaaagtt ttggtggaac gcaatagaac gccggttcgt ttcggttcag
     3661 gatcgcaaca gcgtcccctt caccttcagc tacggttccc ataggatttt ttttcaggtc
     3721 agtaatactg gccgctgtgg ttgtcagaat ttgatagctc ataagccctc cgcttgtctc
     3781 attggcataa tagcatcttt aaaagcactc ttaaaagtgc ttatgttgag aaaaacatca
     3841 gaacaggtga gatatggctc tttccgacat cgccttaaaa cgctctgtaa cgcgttctga
     3901 gcgttttttc ccgtttcgtt gtagtcagct acggtttaac ggtttttaac gccgctcaac
     3961 gctttttagc ggccttgttg ctgtgatctg ttgccatgat ccggatagga gttcaatctt
     4021 gatcaaaatt tcctcaaggc aacgactgtt ttgtaaatac cctctaaaac gcgctgtatc
     4081 gcattctgag cgattttttc gatttcacat ggttttcctc atggttacat cgaacaaagc
     4141 gttagaaagc gattcagggc ttctcagtga aagatccaaa aaggatcaat caacggatcg
     4201 tatgatctga actcttctgg ttttgagaaa ggagacaccg ctcgccgcag ccgaacgacc
     4261 gagtgtagcg agttagtgag cgaggaagcg gaacagcaca gggataacat caggcaatta
     4321 cgcgcagtgg ttaaaaagcg agcatattgt ttttatgtgt ggttgtcttg gttcagagtg
     4381 atctgtcctc tgggtttacg caaagttatc cacaggtagt gtgaattttt tgttttgagc
     4441 aaagttatcc acagattttt tgtgtttttt tgcttattaa acttcttgtt tttaagatct
     4501 taaatgtaga tcggtagatc tactttaaga tctttaactt actctaactt attctgcaaa
     4561 cattgtgcca gtttttttat caataatttc attaagatag gtgttgtttt ttgtcgcctc
     4621 aaaatgaaag gtggatgggt acgcactgaa aggtggatgg gtacgcactg taattttcat
     4681 gaaaggtgga tggctgcgca ctgaaaggtg gatgggtacg cactgaaagg tggatgggta
     4741 cgcatttttc tgtttttatt cagatattcg tatctttgta tcttcatttc tttagttctt
     4801 tatagtgctc taatctaacg tatgacgtta aaaggtggat acctgcgcat ttctatcata
     4861 ttgaatataa atgataaaaa ttctttcatg tgatccgtgt tttggtggaa acatataagg
     4921 tggataaacc actttacata tttccgcctt tgtggttatg atttcaccat aagaacttaa
     4981 aaaccaaagg taatgtatga acttactgag cgttacaaag aaaacaaaaa ttcgccacag
     5041 aaatgaaatt aacagcacat tctcatcgtt gcctttgtct gccagaagaa ttttgtttat
     5101 ggctatggcg cagattgatt cacgtgaaga gttatctgag ggggcaacct ttcgtattac
     5161 ggctcgggaa tatgcattta tcgctgatat tgatgtaaca ggtgcttacc ggcagttgaa
     5221 agagggggca gaagaacttc aggccagtgt tattcgtatt cctcgaaatc aacttctcac
     5281 agctcagggc aatcaaagct ctggacatac gaagaagaga ggtaaattac cttctgatgc
     5341 agtcaggctt atgaacctga ctgcgttttg tgattatgtt gaaagtgaag gatatattga
     5401 tattgctttt tcacatgtca ttgagccata tatcacgatg ttaaaggatt cttacactac
     5461 tcaggttttg atatcgtctg taagactcgc tgatcaaaat tcaaatatgc tttatcagtt
     5521 tatcaggaaa caatacagca gtggtaaaaa gacatttttc gacattgaag tagatgtatt
     5581 aaaggatgaa cttgaattat tcagaataga taatggcgaa aaagtttacc tttatcagaa
     5641 ttttaaggat tttaataagg tttttctgca aaaaaatatt gaaaaaataa atcaatatac
     5701 agagattaat catctcgaag ttaaaattgt taagagagtc gcaagaagag catcaaagct
     5761 aagattttcg tataaaatag ataaagaaag cgaaggactt gataccagaa taccgtatgg
     5821 tttcagaggt gcttaatata taaaggtaaa tacactatgg agcaaataaa attacataaa
     5881 caaggtggtg cggcagtaat tatcattcct gctagtatga tagaaaatac aggatgggaa
     5941 gatggaacct tattggaggt actagaaata aatgagactc ttcatataag aagaaagaca
     6001 gaatcaggag ctttagctac taaagcttgt ccttctcttt tttcatctac tatgaggcat
     6061 gttaattacg ataaaattat taggtagtga ctccaactta ctgatagtgt tttatgttca
     6121 gataatgccc gatgaccttg tcatgcagct ccaccgattt tgagaacgac agcgacttcc
     6181 gtcccagcct tgccagatgt tgtctcagat tcagattatg tcgctcaatg cgctgagtgt
     6241 aacgcttgct gataacgtgc agctttccct tcaggcgtga ttcatacagc ggccagccat
     6301 ccgtcatcca taccacgacc tcaaaggccg acagcaggct cagaagacgc tccagtgtgg
     6361 ccagagtgcg ttcaccgaaa acgtgcgcca caactgtcct ccgtatcctg tcatacgcgt
     6421 aaaacagcca gcgctgacgt gatttagcac cgacgtagcc ccactgttcg tccatttccg
     6481 cgcagacgat gacgtcactg cccggctgta tgcgcgaggt taccgactgc ggcctgagtt
     6541 ttttaaatgg cggaaaatcg tgttgaggcc aacgcccata atgcgggcgg ttgcccggca
     6601 tccgacgcca ttcatggcca tatcaatgat tttctggtgc gtaccgggtt gagaagcggt
     6661 gtaagtgaac tgcagttgcc atgttttacg gcagtgagag cagagatagc gctgatgtcc
     6721 ggcagtgctt ttgccgttac gcaccacccc gtcagtagct gaacaggagg gacagctgat
     6781 agaaacagaa gccactggag cacctcaaaa acaccatcat acactaaatc agtaagttgg
     6841 cagcatcacc aaattattag atataatagc ggattaaatc tgttagttaa tgaactgtca
     6901 ttttcggcaa gaagggtctt ttttatttgt ttatattatt actttgaaaa taaacttgaa
     6961 tcggataagt cgctattgat ccgggctgat aagtatgcag aatttgttgg tgttgagcgt
     7021 tctgttgcat ataagcaaat gaaggatgca gcagatttct tttcaagaaa taaaaattta
     7081 attacacatt gtgactatat ttcgaatgag ggattgcttc gtgttatgtt ttcgtctaag
     7141 acgataaatt atattacagc gatagactcc cgtaaatatc aggttaccga gcttcctcta
     7201 aaatctgctt tgtctcttca aggaagatac tcatggcctt tatatcaact gataaaattt
     7261 agccatctta aatctttcac tataggtgta gatgaactta agaaagaaat aggttatatt
     7321 caaaatcaaa agttttcttt ctttaaaaga gatggaatca atatagctat tgatgaaata
     7381 acagaaagga cagatgttaa aaacattaaa tgtgttaatg ctgaaaggca ggggagaaga
     7441 gtcagcaaag tccgttttga gatcgaaatg aggtaactca tggctaaaat ctatcaattc
     7501 cctcaggggg aagaacgagc taaattcagg aaagaaatcg ccagggagcg taagaaacgt
     7561 tttgcagtga aaacagggag cacttttgtt aagtggcttg gctggggatg gttttatctt
     7621 cgtcttcttg ccgcgagtgt tttacatttt atctttgttt ttgtgttagc cattcttggt
     7681 gcgtttaaat gggctgtgtt ctggctgggg ggtattttgt gtgtcatcac ctggtatcat
     7741 ctcgatcacc agttatggac gccacaaaat ctgactatac cggtgattac ggcaacctgg
     7801 attttgggac tgctggctga gcctctgatg gagctgctca acaaaactat gccctggcac
     7861 aagttacttg ttcccgaacc tgcggacgaa agcaccgaga acagtagcga gataagccag
     7921 caatgatgct actcagcagt tatgccagtc cctgcctttt cactgcgatg caggataggg
     7981 caaacgccgc aaaatgacgt ctctgacgcc attccgcaac gtttgcccgg tgacctacag
     8041 tcggttctta ttggagatgt ttatgaaata tctggtcaaa gagtttatta acgagaaata
     8101 ttctaaggct gttaatattt taaaggataa tcttaaagaa cactatcatg ttttttatgg
     8161 tgtgagatta agtgagattc tttttcctac cagtgaatat ggcacagatg ctttctttaa
     8221 agaatttgaa ttaattaaca gtgtaatact tccattagta atatttgatt ttatagaccg
     8281 aaagccgatc atggttatcg gctttgagga agcgcctggg attgattcac ttattgactc
     8341 tggtatggaa gttgttttgt tagatggttt aagcgatctg ctactggttg aaaaactgat
     8401 gccattgttt gattaaatcc ctggaaatac atgttaccca ttcgtattaa tgcattttac
     8461 acgaacgatg tttattccat ttacctgttt tcataacttc tcccctacgg agaactaacc
     8521 aaactgatct gcatctatga tcttaattat ataaaatttg gggggcgtga ttctaatgtc
     8581 ctcattgtat tggtaacaat ctttttttca tcgtttttat tttgatattt aatattgctc
     8641 tcaattgatt tttaccgttt cttcattcaa tgatatgagc gtcaaattga ttagataatg
     8701 gtgattttta ccctctatca tcaaagcttg tgaagctatt aatatacaaa aaattttcag
     8761 tcgtttttct attgtctcac atatttttaa gagagaatct gtatgctgtt cgccattcac
     8821 ttatgtaggc cacttcgtac taatcgcttc aatctaaaga acttcacttt tccttagcgt
     8881 ttgttatcgc agaaattccc acggaacccc taagacggta aaaggggtgc cggcgtacaa
     8941 tccctaaaag atattaaacg aaattttgga ttatataatc agctaaaacg tcatgttctg
     9001 ccgacatagt ctgcattagg gctttctgag gtcctgacca agcccctctg gccaacaccc
     9061 ccctagtagg tgctacggta gataaatatc tgagcgggac cccctgtaag atactatctg
     9121 gagccataac atttaggtct tcaacttttg cgacaggagt gtctggagta aaagaagcca
     9181 ctgctgagta tgtttttata gatgctaatt tggggcgaag gtcatttttt actgaatcaa
     9241 ttattttatc tctatgggct gcatcagccg cgatctctga tgagttttgt tggcctggat
     9301 tctttctttt atcaaatccg ttgaagaccc aacctgcaaa tttaggtgaa cctttttggc
     9361 ttattagaga gttattttgg gctgaagttc ttctaatacc ctgttcccag tcgtctctaa
     9421 attttggtaa agtttcacca atgagtccta tgcagtaagc cgaaaatata tcggcagtac
     9481 atggaaccaa gaaataatca gaacagtaga gagccgatct gactatcgta ttaaatgacg
     9541 gcggtacatc aataagaaca taatcgtatt cacaattatt taactcttga gctttactag
     9601 caattaaact aggtatcaag aatctgttta ctgcgtttcc agcaattaaa tctgtaccta
     9661 cactaatcac atctgaaaga ttattgagcc aaaagtcacc tggagctatg tctaaccttc
     9721 ctgagccagg ctccatggga aattgtggtg ggaatgtatg tacgacaggg agaatatctt
     9781 gttgtataaa tggttggcaa aatgctctta tcgttttacc ataaggatta tttgcatcct
     9841 tctttaaaag gtcagagaag ccagtctcac caagtaaagc tattgataaa ttgcattgag
     9901 gatcgaaatc tatcagcaaa acagattttc cctttgccga tagagataca cttaagttcc
     9961 aagtcagggt cgtttttcct accccgcctt tgttgttaaa tagtgaaata gttttcattt
    10021 tattcctttc ttagacattt tcaatgttta gaaataacta catcggagaa tagtgtaata
    10081 cgtaatcgat tgcttgatac ccgtatataa ccatcaacat gcacgacaaa ccttaaaatt
    10141 gaagtttccg tacagtttct cattccgaca gagtttgtca cgcaaaaatt tacaggttta
    10201 tagatttaat tttctttaac taactgcagg ggctgaagat ttctttcagt gagaaatcgc
    10261 ttagcgggtg ttatcacaga aattcccacg gaccccattt tgtccgaact cctactgata
    10321 gattaaatcc tgaatcatct aagttttgca gaatcaagta actattggct gttgtacata
    10381 gccagggctg ctttgagaac agttgttttg cgtcggcctg tctgttcgct tcggcttcca
    10441 attattcgtt ctctttgagc cagttatcta caagctggtt caccacgtca gtgattgatg
    10501 tacctttcct ggcgcatgca gccttaaacc gggtgtgctt ttcttcatca aaattaacgt
    10561 taacacgttt gatttttcca gatgatactg gtgctgttac tactcgttcc agatctctgt
    10621 tttcaccaaa ggtcattttt tttactgacg tatgcgcttt ttcaagtgac attcaggcta
    10681 ctccggtttt tatacttatt tgattatatg agtaaatgtg taattactca aacattctaa
    10741 ctatctcttt tgtaaggatt tctatttcac cttttgcagc accatcactg gattcaaaca
    10801 cgctgtcgcc atcaagaatt gatttcacgt aaacctgacg ttgtgtaata gctgtacgaa
    10861 atgctttaac accggtgtct ttgatacttt ctttaagcac attcaacatg gttgccattt
    10921 ctatcttacg ggtgatcaga aagcgggctt caactttgcg gctgtaagcc tgtgcttcca
    10981 gaacagtaac gacgcttcct gctgcggaga aatccagagg gctgggggtg acagggataa
    11041 ttaccagatc gcttaccatg acggctgcgg aggtgattac tgagagtgaa cctgccccgt
    11101 ccacaatagc aaagtcatag tccgccagat cttttcggat tccatagacg tctttttcag
    11161 atgctgctgt aaatacgtca aatgctgcct tgcccgcttt gctccagttc gtcaggctca
    11221 tttgtggatc tgtatctacc acagcaatgt tgtatccgct cctgctcaac gcagtggcta
    11281 tgttgattac ggcagttgtt ttacctgaac cccctttcgg attcagaaat gagatcacct
    11341 tcatactcaa atcctcaatt gtgtgtctac tcaattactc ctgtgagttt ttgagtttat
    11401 catactcttt gtatgagcaa aggttaatat gagtttatgt gtatatgagt taaggagtat
    11461 ctactcattt gagttgtctt gtgtatatga gtgtttgagt ttatgagtgt gtagtgacaa
    11521 ggtcacatct actcattatt tgttttttgg ttcttttaga gtgtgccttt cctgtcgatc
    11581 cactttctgg ttcattttgg gtatagtggt gtgaccttat gcgaactggt tctgtaatat
    11641 gacttcaaat gcaccaacaa actttgtccg cgcttatctg agagcttcaa cagcagaaca
    11701 ggatgcttcg cgtgccctgg aaactatcga ggcttttgcc agggaaagag gcctgatcat
    11761 ttgcagctat tatattgaga atgaatccgg ctcccgtctt gaccgcccgg aactctttcg
    11821 tcttctgaga gattgccagc aaaatgacat tttgcttatc gaggatgtgg acagactttc
    11881 ccgactgtca ggggaagact ggaatacgct gaagaaaatg atccgtcaga aagatatccg
    11941 ggttatggcc gtgaatgtgc cgacgacctg gattaattct ggtatgagtg agtttgacag
    12001 ccggttgttt gcagccatta acgatatgct gctggatatg ctggccgctg ttgccagaag
    12061 ggactatgaa caacggcggg aacgccagaa acaggggata gagaaagcaa ggaaagacgg
    12121 caaatataaa gggcgtaaac ctaatcaagc cagacatgat gcaattatca ggttgataga
    12181 aagtggcagc tcctggacac aggtacagaa ggtgctaggg tgcagccgcg gcacaatcag
    12241 tagcgcaata aaacgtaaat ccctgcaatc ctccggtgag tagggtaggg tgagctcaat
    12301 aaactggttg acgttattta tgagggaggt gctgaagtgg atatttttaa aaagcaaatt
    12361 gaatgttgta actaatgagt atcaaaataa aaggccgctt acgcgacctc agccttttgc
    12421 ggttttggct tggtgatata ccacgataga caaccgacaa taaaactcat gaacattcca
    12481 gtaaaacgat catcgatgcc tggaaacagg caaccgacaa acatcgcaac agggaaagga
    12541 agaagtaaac agcgccccaa aatgctcata aaatccataa cacctccttt gttaaagggc
    12601 aggaagcggt gcacttcctg cccttagtta cgtttccgca gtcatcaatc gatgaactga
    12661 cggatcagtt ccagaactgc aatcagcgcg tttgcaagcg cagctaccgc cctcagaacg
    12721 atgattaaca ggttaatcat ccgttttctc cataaatgga gtaaagacac cgtggcttgc
    12781 tcttacgctg cctgtcacca cggcttctct tcgtcttgtc agttgtagag agtctcactc
    12841 ctaccagagc acagataacc agcaccacct gtattacctg tcaggacttt gaagctgttc
    12901 atgccgcatt tagcggaatg cagggaaaga ctcaccacag cactaaccct ctgattatac
    12961 gtttatctaa ctttcaagca aaccgttaac gcgtagtcac cgcctgtcat attgcataat
    13021 caggcttcac ggattatcat actgttgtca gttgtagaga gtttctcttc cgggcgagtt
    13081 accagctcgt accggaactg taaaaaagcc gacctcacca gtcggttttt ttacgtctgc
    13141 agtttcccgc ttcatttaga tctattttaa atgaataaat tttctgcggc aaacccaacg
    13201 acgttgggtc gttagtgtaa ataattgttc aaaactcata aacactcctt tgttaaaggg
    13261 caggaagcgg taaacgtctc tcccttagtt aagccatcaa tcactcgatt agtttctgaa
    13321 tggcaatcag cgcgtttgca atggcgagca cagccctgag aacgatgatt aacagattca
    13381 tcatccgttt ctctaggttg atactggagg aagtcaccgt agcttgctca tacatcgcct
    13441 gtcacaacgg ctctctcaca ttgttggatg tagagtacct tccctctacc agagcactga
    13501 acacagcacc acccgtttgt tcagccagga ctttaattcg ttcatcccca gatagcgcag
    13561 gggttagaag caagggagac acactgtaca gcgccaacac aaagattata cagatgcatg
    13621 cataaaattc atttaaagaa attaccattg acagacaatc tgaagtcaaa ctagacaaca
    13681 ggtgtctagt gccataatac tatgagtgtt gggtgtagag tatcttcctc cagtcagagc
    13741 caccaactca aggctggaaa acacaaaaaa gccaaattac cagtcggttt tttttgtgct
    13801 tgtcgttcag ctgtacttcc gttttttccc cccttcattg aagaagtagt aggcagagca
    13861 ggtaatgact ccaacttatt gatagtgttt tatgttcaga taatgcccga tgactttgtc
    13921 atgcagctcc accgattttg agaacgacag cgacttccgt cccagccgtg ccaggtgctg
    13981 cctcagattc aggttatgcc gctcaattcg ctgcgtatat cgcttgctga ttacgtgcag
    14041 ctttcccttc aggcgggatt catacagcgg ccagccatcc gtcatcggca ctgttgcaaa
    14101 tagtcggtgg tgataaactt atcatcccct tttgctgatg gagctgcaca tgaacccatt
    14161 caaaggccgg cattttcagc gtgacatcat tctgtgggcc gtacgctggt actgcaaata
    14221 cggcatcagt taccgtgagc tgcaggagat gctggctgaa cgcggagtga atgtcgatca
    14281 ctccacgatt taccgctggg ttcagcgtta tgcgcctgaa atggaaaaac ggctgcgctg
    14341 gtactggcgt aacccttccg atctttgccc gtggcacatg gatgaaacct acgtgaaggt
    14401 caatggccgc tgggcgtatc tgtaccgggc cgtcgacagc cggggccgca ctgtcgattt
    14461 ttatctctcc tcccgtcgta acagcaaagc tgcataccgg tttctgggta aaatcctcaa
    14521 caacgtgaag aagtggcaga tcccacgatt catcaacacg gataaagcgc ccgcctatgg
    14581 tcgcgcgctt gctctgctca aacgcgaagg ccggtgcccg tctgacgttg aacaccgaca
    14641 gattaagtac cggaacaacg tgattgaatg cgatcatggc aaactgaaac ggataatcaa
    14701 cgccacgctg ggatttaaat ccatgaagac ggcttacgcc accatcaaag gtattgaggt
    14761 gatgcgtgca ctacgcaaag gccaggcctc agcattttat tatggtgatc ccctgggcga
    14821 aatgcgcctg gtaagcagag tttttgaaat gtaaggcctt tgaataagac aaaaggctgc
    14881 ctcatcgcta actttgcaac agtgccgtcg ttacagacag gttgctgcac gtctttgcga
    14941 aaatgaggct gtcgaactcg atgtttctgg cccgaagccc cggttgacaa tttctcccct
    15001 cgccagtctt gatgagccgg acagtctgaa acgactgagc aaaatgatca gtgatctact
    15061 ccctccggtg gatttaacgg agttgctgct cgaaattaac gcccataccg gatttgctga
    15121 tgagtttttc catgctagtg aagccagtgc cagagttgat gatctgcccg tcagcatcag
    15181 cgccgtgctg atggctgaag cctgcaatat cggtctggaa ccactgatca gatcaaatgt
    15241 tcctgcactg acccgacacc ggctgaactg gacaaaagcg aactatctgc gggctgaaac
    15301 tatcaccagc gctaatgcca gactggttga ttttcaggca acgctgccac tggcacagat
    15361 atggggtgga ggagaagtgg catctgcaga tggaatgcgc tttgttacgc cagtcagaac
    15421 aatcaatgcc ggaccgaacc gcaaatactt tggtaataac agagggatca cctggtacaa
    15481 ctttgtgtcc gatcagtatt ccggctttca tggcatcgtt ataccgggga cgctgaggga
    15541 ctctatcttt gtgctggaag gtcttctgga acaggagacc gggctgaatc caaccgaaat
    15601 tatgaccgat acagcaggtg ccagcgaact tgtctttggc cttttctggc tgctgggata
    15661 ccagttttct ccacgcctgg ctgatgccgg tgcttcggtt ttctggcgaa tggaccatga
    15721 tgccgactat ggcgtgctga atgatattgc cagagggcaa tcagatcccc gaaaaatagt
    15781 ccttcagtgg gacgaaatga tccggaccgc tggctccctg aagctgggca aagtacaggt
    15841 ttcagtgctg gtccgttcat tgctgaaaag tgaacgtcct tccggactga ctcaggcaat
    15901 cattgaagtg gggcgcatca acaaaacgct gtatctgctt aattatattg atgatgaaga
    15961 ttaccgccgg cgcattctga cccagcttaa tcggggagaa agtcgccatg ccgttgccag
    16021 agccatctgt cacggtcaaa aaggtgagat aagaaaacga tataccgacg gtcaggaaga
    16081 tcaactgggc acactggggc tggtcactaa cgccgtcgtg ttatggaaca ctatttatat
    16141 gcaggcagcc ctggatcatc tccgggcgca gggtgaaaca ctgaatgatg aagatatcgc
    16201 acgcctctcc ccgctttgcc acggacatat caatatgctc ggccattatt ccttcacgct
    16261 ggcagaactg gtgaccaaag gacatctgag accattaaaa gaggcgtcag aggcagaaaa
    16321 cgttgcttaa cgtgagtttt cgttccactg agcgtcagac ccctgttaag accgatctga
    16381 gcacgccgct gcatgtccgt atccaggatc cactcaataa tgaaaagggg gcactgttgc
    16441 aaagttagcg atgaggcagc cttttgtctt attcaaaggc cttacatttc aaaaactctg
    16501 cttaccaggc gcatttcgcc caggggatca ccataataaa atgctgaggc ctggcctttg
    16561 cgtagtgcac gcatcacctc aatacctttg atggtggcgt aagccgtctt catggattta
    16621 aatcccagcg tggcgttgat tatccgtttc agtttgccat gatcgcattc aatcacgttg
    16681 ttccggtact taatctgtcg gtgttcaacg tcagacgggc accggccttc gcgtttgagc
    16741 agagcaagcg cgcgaccata ggcgggcgct ttatccgtgt tgatgaatcg tgggatctgc
    16801 cacttcttca cgttgttgag gattttaccc agaaaccggt atgcagcttt gctgttacga
    16861 cgggaggaga gataaaaatc gacagtgcgg ccccggctgt cgacggcccg gtacagatac
    16921 gcccagcggc cattgacctt cacgtaggtt tcatccatgt gccacgggca aagatcggaa
    16981 gggttacgcc agtaccagcg cagccgtttt tccatttcag gcgcataacg ctgaacccag
    17041 cggtaaatcg tggagtgatc gacattcact ccgcgttcag ccagcatctc ctgcagctca
    17101 cggtaactga tgccgtattt gcagtaccag cgtacggccc acagaatgat gtcacgctga
    17161 aaatgccggc ctttgaatgg gttcatgtgc agctccatca gcaaaagggg atgataagtt
    17221 tatcaccacc gactatttgc aacagtgccg ccggtagaac tccgcgaggt cgtccagcct
    17281 caggcagcag ctgaaccaac tcgcgagggg atcgagcccg gggtgggcga agaactccag
    17341 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
    17401 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
    17461 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
    17521 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
    17581 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg
    17641 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
    17701 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc
    17761 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
    17821 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
    17881 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
    17941 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
    18001 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
    18061 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg
    18121 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
    18181 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
    18241 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
    18301 catcctgtct cttgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa
    18361 gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc agctggcaat
    18421 tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt aagcccactg
    18481 caagctacct gctttctctt tgcgcttgcg ttttcccttg
//
