LOCUS       NZ_CP091663             2101 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli strain KFu023 plasmid pKFu023_1, complete sequence.
ACCESSION   NZ_CP091663
VERSION     NZ_CP091663.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25236400
            Assembly: GCF_022494645.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Gomi,R.
  TITLE     Genome sequencing of blaCTX-M-carrying Escherichia coli isolates
            recovered from river water in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Gomi,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2022) Department of Environmental Engineering,
            Kyoto University, Kyotodaigakukatsura, Nishikyo-Ku, Kyoto-City
            615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022494645.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 03:25:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,393
            Genes (coding)                    :: 4,206
            CDSs (with protein)               :: 4,206
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 187
            CDSs (without protein)            :: 187
            Pseudo Genes (ambiguous residues) :: 0 of 187
            Pseudo Genes (frameshifted)       :: 57 of 187
            Pseudo Genes (incomplete)         :: 127 of 187
            Pseudo Genes (internal stop)      :: 40 of 187
            Pseudo Genes (multiple problems)  :: 34 of 187
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091663.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="KFu023"
                     /isolation_source="river water"
                     /db_xref="taxon:562"
                     /plasmid="pKFu023_1"
                     /geo_loc_name="Japan"
                     /lat_lon="34.6 N 135.7 E"
                     /collection_date="2013"
                     /collected_by="Kyoto University"
     gene            join(1570..2101,1..434)
                     /locus_tag="L6L71_RS22455"
                     /old_locus_tag="L6L71_22415"
     CDS             join(1570..2101,1..434)
                     /locus_tag="L6L71_RS22455"
                     /old_locus_tag="L6L71_22415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            438..704
                     /locus_tag="L6L71_RS22460"
                     /old_locus_tag="L6L71_22420"
     CDS             438..704
                     /locus_tag="L6L71_RS22460"
                     /old_locus_tag="L6L71_22420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          2..94
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.81e-41"
ORIGIN
        1 cttggtttaa agggaagaac tacgttaagc acgaacgttg ggtagaactc tggcgcgatt
       61 gcttgcgggt gaactatgag ccgaatatcg atattcgggc agtaaaaact aagacaggtg
      121 aggttgtggc caacgttgcc gagcaactgc aaagcgcggt tgctgaaacg ctgaaatact
      181 ccgttaaacc ggaagatatg gcaaacgatc ctgagtggtt tcttgagctg acgcggcagc
      241 ttcacaagcg ccgttttatc tcgaccggtg gggcgctaaa aaacgtcctc cagttggatc
      301 gagaaaccaa tgaggatctt gtcattgccg acgatgtagg ggatggcact gatgacggga
      361 agcggacggc gtttgtctgg gattcaggta aacggcgtta caaacgcgcc cctgagaagg
      421 ataaatcgga ttaacgtatg aatattaata ttgaatacct gaatggaaat aagactattg
      481 gtttattttt tttaagaagt gaagcggtga ttcctgacag gtttaaaaac cttattttgc
      541 ttattgatgg attaagtttt ggcacatttg gttttcatcc gcacgaaggt tttgaggatg
      601 aattaatttt atatattcag aaaacaaacg agagggtaaa aactcttttt gtgaaaatag
      661 atttaaacgg tatcaagttt ggatttttaa gaacgcattc ttagttctgg aaaagagcca
      721 gcggcaggct gaggtgatag gtacgagatt gcatgcaatc tctagtgctc tgtctatcct
      781 gcattatcct cagcattatc ctcagcatta tcctcagcct tgccaactcg acaccaatgc
      841 aggatagaca atccgatgtc aaatgttaac actctgcgag tggtacattt tccccggatt
      901 atcgtcctga gcctgccgct ggctctcttt ctaccgcctc gctttgctcg ttgctcaacg
      961 cctcacagac acggattaaa atccgcatcc gttcaccgtt ttttaaagtc cgttaaaagc
     1021 atgatgccat ctccgagagt taatctcgtc aaatgctaaa tcgtgggggt cccctttggg
     1081 gttccgattt agtgattgac gacaccaccg attaaaaaac ttatgcgggg tggatggttt
     1141 cacgaagtga ggccatccac ctgtaagaca gggttttgtt tttattccct gttttggtga
     1201 tcgggtgtgt ggaaaaggtt ggggtaagcc gttcgggggt gcttgttttg gggggttaaa
     1261 attgtggtta ttttttgcgc aattctcgcg cgtgatcctt gtatttatac ttaagggata
     1321 aatggcggat atgaaatagt ggtttagccc agtaatgacg aggctttgag tgggttttga
     1381 caggtcaaag aaaatggagc agaattgagg cgtttttaat cggcgttggg gagtgcgtca
     1441 acactcccca acatttcgaa tgtgtcacct cagcggcaaa ctctggtgac atgtactggc
     1501 tcgcaatgca caggtacgtg atgaatatac cacatcaaat cacagcctgc ccagatcgga
     1561 gcaggcttaa tgtcagaaga taaattcctt tcggactaca gcccccgtga tgcagtttgg
     1621 gatacccagc gcacgcttac cgattctgtc gggggtatct accagactgc tgctgaattc
     1681 gagcgctatg cactccgtat ggcctcctgt agcggtttgt tacgttttgg ttggtctacc
     1741 atcatggaaa ccggagaaac gcgcctacgg cttcgtagtg cgcaattttg ccgtgtccgt
     1801 cattgccctg tctgccagtg gagaagaacc ctcatgtggc aagcccgttt ttatcaggct
     1861 ctaccgaaaa tcgttgtgga ttacccgtct tcccgatggt tgtttctgac gttaactgtc
     1921 aggaactgcg agataggtga acttggaaca gtccttacag caatgaatgc ggcgtttaag
     1981 cgaatggaaa agcgaaagga gctatcacct gttcaggggt ggatcagggc tacggaggtg
     2041 acgcgaggta aggatggcag cgcacatccg cattttcact gtctgctgat ggtgcaacct
     2101 t
//
