LOCUS       NZ_CP091603             6141 bp    DNA     linear   CON 17-MAR-2024
DEFINITION  Klebsiella pneumoniae strain Nord77_R886 plasmid pR886_4.
ACCESSION   NZ_CP091603
VERSION     NZ_CP091603.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25263436
            Assembly: GCF_021899755.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Braun,S.D.
  TITLE     project DRESI
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Braun,S.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2022) Optical Molecular Diagnostics and System
            Technology, Leibniz Institute of Photonic Technology, Albert
            Einstein Strasse 9, Jena 07745, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021899755.1-RS_2024_03_17
            Annotation Date                   :: 03/17/2024 03:37:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,854
            CDSs (total)                      :: 5,733
            Genes (coding)                    :: 5,475
            CDSs (with protein)               :: 5,475
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 136 of 258
            Pseudo Genes (incomplete)         :: 129 of 258
            Pseudo Genes (internal stop)      :: 27 of 258
            Pseudo Genes (multiple problems)  :: 29 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091603.1.
            Bacteria and source DNA available from Sascha D. Braun.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Nord77_R886"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pR886_4"
                     /geo_loc_name="Germany"
                     /collection_date="2018-02-18"
     gene            <133..285
                     /locus_tag="L5469_RS29170"
                     /old_locus_tag="L5469_29170"
                     /pseudo=""
     CDS             <133..285
                     /locus_tag="L5469_RS29170"
                     /old_locus_tag="L5469_29170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            complement(692..1489)
                     /locus_tag="L5469_RS29175"
                     /old_locus_tag="L5469_29175"
     CDS             complement(692..1489)
                     /locus_tag="L5469_RS29175"
                     /old_locus_tag="L5469_29175"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="WP_043907054.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            1833..2114
                     /locus_tag="L5469_RS29180"
                     /old_locus_tag="L5469_29180"
     CDS             1833..2114
                     /locus_tag="L5469_RS29180"
                     /old_locus_tag="L5469_29180"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            complement(2396..3850)
                     /locus_tag="L5469_RS29185"
                     /old_locus_tag="L5469_29185"
     CDS             complement(2396..3850)
                     /locus_tag="L5469_RS29185"
                     /old_locus_tag="L5469_29185"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3831..4169)
                     /locus_tag="L5469_RS29190"
                     /old_locus_tag="L5469_29190"
     CDS             complement(3831..4169)
                     /locus_tag="L5469_RS29190"
                     /old_locus_tag="L5469_29190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4151..4543)
                     /locus_tag="L5469_RS29195"
                     /old_locus_tag="L5469_29195"
     CDS             complement(4151..4543)
                     /locus_tag="L5469_RS29195"
                     /old_locus_tag="L5469_29195"
                     /inference="COORDINATES: protein motif:HMM:NF037437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            complement(4951..5898)
                     /locus_tag="L5469_RS29200"
                     /old_locus_tag="L5469_29200"
     CDS             complement(4951..5898)
                     /locus_tag="L5469_RS29200"
                     /old_locus_tag="L5469_29200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     ARG             complement(696..1489)
                     /name="blaOXA-232"
                     /gene="blaOXA-232"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(4965..5886)
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(2400..3850)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttgatctac gcctctaact aggtcgatcc ttgggattga gtggcaccat tgtcagtgca
       61 agaatactga tccatataag tctctctagt cggacaacac caagtgttga agagatcaaa
      121 aatcaaccta tatcggagga ttcatgtctg acattgacag atgcccccat gaaagcaaag
      181 cgaatgcggt cccaaagcgc ctctgtagaa acgaatttga gcgaggcatt tgatgccatc
      241 gtctgcgtcc caagcgccgg caaggacggc ctggttgacc tatagtaaac cggtagatga
      301 aaatgatgga gaaccaatat cagcgccttc ttggactttt tcgtcaaacg ctttagcagc
      361 caagaaagcc tacaatcctt gatgggctag gcactttatc gcagcggcat tcacctttac
      421 tgacatcgcc actcctttat tcacaccccc atgttgagtg accatggcca ttgagataac
      481 aaaaaagcct agcaaattgg ctaggctttt tacgtttagg aatgtgatgc taatgctcag
      541 gctgaaagtt aacgcgcgga gcttaagtcg attaggcctg accgattaag tatgagccat
      601 taactacaag cgcatcgagc atcagcattt tgtccatata tctttacagc ttatatgacg
      661 gctatttcac acaaatacgc gctaaccact tctagggaat aattttctcc tgtttgagca
      721 cttcttttgt gatggcttgg cgcagcccta aaccatccga tgtgggcata tccatattca
      781 tcgcaaaaaa ccacacatta tcatcaagtt caacccaacc aacccaccag ccaatcttag
      841 gttcgatact agtcgagtat cccgttttag cccgaataat atagtcgcca ttggcttcgg
      901 tcagcatggc ttgtttcacg atgcgctgac tacgctcaga aacgtgcagc ttgttgtgat
      961 acagcttgcg taaaaaagcg atttgctggg tagccgaaat gcgaatacca ccatcgagcc
     1021 aaaaactgtc tacattgccc gagatatcct cattgccata atcgaaggcg tgcagcattt
     1081 tactcatacg tgcctcacca atttggcggg caaattcttg ataaacaggc acaactgagt
     1141 acttcatcgc ggtaattaag tcatggtcac gattccaagc ggcgatatca cgcgtctgtc
     1201 catcccactt aaagacttgg tgttcatcct taaccacgcc caaatcgagg gcgatcaagc
     1261 tattgggaat tttaaaggta gatgcgggta aaaatgcttg gttcgcccgt ttaagattat
     1321 tggtaaatcc ttgctgctta ttctcattcc agagcacaac tacgccctgt gatttatgtt
     1381 cagtaaagtg agcattccaa cttttgtttt cttgccattc ctttgctacc gctggcattc
     1441 cgataatcga tgccaccaaa aacacagccg ataaggctaa tacacgcata acgtcccctt
     1501 gcttaatgca atatatccat acaaattaag tgctaagctt atcattctct gccctctcat
     1561 tccctagcaa actgctgacc aattgtggca acaatcaggc acaagcttta actattacct
     1621 aaattccacg tgtgtttttt attagcttca aaaatcacta tttcacgaag aatttagact
     1681 gcttctcaca cattgtaaca ttatttacaa ccacctttca atcatttttg ataaatcatt
     1741 gatttcatct ttgctgcaat gatacttaat aaactctgca agttatccac agagcaacac
     1801 tcaattttat tgatgatatt cttaggggga gtatgggatt ttttaaccta tctaaagcgc
     1861 ttgaggaggt gtctgatgct tttggggcta aagaaaccgc aatcgctagt gccaagttgt
     1921 taggtaaggg agttgttaac ctagctcatt ttgcagcaac aacaggtttg gattcggtgg
     1981 tcaaacaaac gagcaacaaa gttctcaaca gcgagtcttc aaccgaagaa caacgagaac
     2041 aggctaaaaa gtctgccgca tgggcagacg cgagaatcgc cgctagaaat aaagcagaaa
     2101 acaccgaaga ctagctgaac gcacatttcg acaatgatgt ggactacatc gttgatcata
     2161 acttcacggt gcgctagctt ccatagaagc caactaataa caaggagtta gcatgtctga
     2221 ttcagatttc aaaaatagcc ctgagtatga tgactggatt gaatctggtg gtagagatga
     2281 agattacagt tactactatg aaaaatggca tagaagaact caacgataat tcccgccgat
     2341 aaaaggaaag tgatagcact ttcctttttt tataactgac ggccgttttg ttccgtcaac
     2401 gctcaaagcc tcgcgatctg tctccacgct cccagcctcg atccagtgcc ttcaggagcc
     2461 tgtgttccct ttcttttgca acttgctgac tagcccggtc aagccctcca gctgcttgct
     2521 caagccgtcc acttgctgcg ttaagccgtt cacctgcctc gtcaatcgct ctgctgctgc
     2581 cctgctgatc tcggcctctt gtttggtgct ctcgtacatg ctcgccaagt tcgcgtagct
     2641 ttcctgccat gccttgtgct gctgctcgta tgcgctctgc aaagcctcga acgcgcagag
     2701 caattcccgc tccaatcccg tcatttagta ccccttcggc acgatccaat aggtatcctt
     2761 cccccaaacg gttggattgg ggtcggagat ctgcacgcaa aggtgctcca atttcttccc
     2821 gcactgggtc agctttagct tgccgccctt ctctgccaag gcttggagag tggcttgccg
     2881 ctccgctatc aattccccct gataccagag gatcccgctc gttgcgctca agaacaggac
     2941 gatgctgacc agcatccaga tccacgtttt gagcaacagc cagcccattt tctggctgtg
     3001 ctcggcaata gcggcgctca ttttctcctc gcttgatttc aacgccccgg agatagcttt
     3061 ctcgtgcctc gtgaattctt cggtcagacg cttctcgata gcgttggcct gctgcttcga
     3121 gctctgctcg aagtccttcg ccatgtctaa aatcttgctc atacagcacc ccctttaatc
     3181 ggatgttccg ccccccgtcc gggtcggcaa tgctcaggct gtttttggtt tcgcgtacca
     3241 cgctgaaacc cgcaccctgc aaggctttgc gcacatcatc gcggctctgg atctcccctg
     3301 ctccggccat gcgtaaaagc ccgtctgtaa tggcctgagc ggcctccttg tgccccctcg
     3361 gcaaatcctt tggcgtgata acggcctgac ggtttaacgg atcgtccggg tcgtgcaatt
     3421 tcagctcccc gtttaccacg gttcgccacg catcgaggcg cggattgtcg gccttgtgga
     3481 agtagggttg cagccgcttc ccgctctgca attcgatgtt cgggactaca aaattcagct
     3541 ccagccgccc cttgtcgcgg tgctctaccc acaaaacagc gtactggttg ccctccagtc
     3601 cgggcaggag cgccttttca aagtcgctca tcagcttgtc cttggtcgcc ctgggtaaat
     3661 cagcctcggc aaacgacaga acgccagagg tgtatttttt ggcgtaggga ctggaatcaa
     3721 tcagggcttc aatcaccgca gggttgcccc ggtcgagcgt tgcgccttcc cggttcccgt
     3781 cacggcccaa caagtagcca acaggcccgg agccccggcc taccccacgg ttatggaatt
     3841 tgatgatcat ggtgcagttc ctccagctcg cggcctatag cggacagctc cgctatgatg
     3901 cgcagccgat ccaacggccc ccactccccg gaattaacgc gccgggcgat ctggttcagg
     3961 ttgttgccca ttccggccaa ctgccgcaag agcgccggat ctacggtagg cgccggcgtt
     4021 cggcgctggg gttgcgcaac accgagacat tgctcccgca tccactccgc cagttgggcc
     4081 ttggggcaga gctcccgcag gcgctcaagc tccgcatctg agacccggat cttgatagag
     4141 cggaggcgct ttaacggctg gccagccatg cagaggcctc cgctaaggcg tcctcataga
     4201 ggctatcgag tagcgtctgg agcgcctttt catcccgaaa atcgcagtgc tccggcaagg
     4261 ggaaaacacc cccgccgacc tcttcgccgt tctccagcaa acgcagattc cagcccccgc
     4321 caagaccgag agtgcggggg gtgatttcgt agtcgtattc cgttgttgtg ggttcgtcac
     4381 ttaccatagc taacacctca tcggtggaaa tttttccatt gatataatct tcaagtagaa
     4441 ttttgaattc atcggttatg gcctccccct ctagttggaa actagaaata acgccattga
     4501 ctgccttcct tctcgaatct ttctctatct gtgttgttga catagggcct cgtccattct
     4561 ccagagccaa gaagccaccg cgcagaccgt cccgaagggg cggatcgagc gggggccaca
     4621 gggggtttcg ggggggcgta gcacccctga acaaggccac agaggcccga gaccgcgaag
     4681 caggtagagg ggcctctgtg tagccttgct tagtacatta ttcaaccatg ggcgggatac
     4741 tgcaagccct agagcaagat caagggaaag agcggctccg tggataactg ctgccgccca
     4801 caccccccga aaaaacaggg gggcgatgga cggacaggcg cagttgtcca cctcgccaaa
     4861 gatccgcgct aaaaggattt tttaaggcta aaagacaaga gcgcaggaac ccccctaaag
     4921 ggggctatta tctgataagg cttgtttcga tcacggacga tcacgcttta ggtaaacgct
     4981 caccgtactg ggggagattt gaagatcgtc cgctatatcg cggttggtgt acccctgagc
     5041 cttaagccgc agaacctccg gcaatagatc ggacttgtcc ttaccgctat tgctcggcct
     5101 tccccctccc ttggatacct tgccgccctt cgcacctctc cgggcctgct cctctctgaa
     5161 ccctgcgggg cttaggtgct ggtgcgtcca tttggcaatg gacttggccg tgtgccggac
     5221 ttcgtttgcg gggagcggct gctcgaactg ggcgttatat cctgatgccc ggtcaacaca
     5281 agcggccaac cattcggggt aatccggcca gccctgccgg atcgctttat aggcccacag
     5341 gcgcgttttc tcgaacaggg tacagtttcg cccaagcccg tactcaggaa gcgatttgcg
     5401 gccattgtag gagctcagat cgaggtaatc ggatagccag gctaaatcgt agagccgggg
     5461 ttcccatacc tgcaccagcc agtgctcatg cagggggttc ttgcagatca gcccggagta
     5521 gcccatatcc gcccctaatt tctcccggag cgcggcctcg atggcggcgg cataccgcag
     5581 aggggctgca tttccatccg gggcagtgcg gatcggcgta tccagtccgt agatcagatg
     5641 cgcgtggccg ttggccttgt tcgtgacggt aatgttaggg gctggagcgc ctacgtcatc
     5701 ccaagataac gtagcggctg catggtctac gtcaaaaacg atccagtggc ggtgcgttgg
     5761 cccgttcggc tggatgtaac gcgcaccaag ggcgcgggca acatccgcaa tccgtaggcc
     5821 cgttgtaagc tcgtcggaat agtagggttt ccggggcagg cgctcccgaa acagctcaag
     5881 ctgtgcgcac tttctcaagc accaccgcgc ttattggcgg cgtggggttg tttggcatga
     5941 tccatgtgat agacttctct tgtgattcgt cacggccccc gtttttcgca gctccgccaa
     6001 gatgcaagcg ataacggggg gttgtctttt ctggacaata cgttacagca ggcacatcct
     6061 agagagcaac gttagttgca tgtaaccttt caccctggaa catatttcca cctccttctg
     6121 cttctgcgct gttttgaatt g
//
