LOCUS       NZ_CP091597            11378 bp    DNA     circular CON 17-MAR-2024
DEFINITION  Acinetobacter baumannii strain Nord4-2 plasmid pR32_3, complete
            sequence.
ACCESSION   NZ_CP091597
VERSION     NZ_CP091597.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25263435
            Assembly: GCF_021899715.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11378)
  AUTHORS   Braun,S.D.
  TITLE     project DRESI
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11378)
  AUTHORS   Braun,S.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2022) Optical Molecular Diagnostics and System
            Technology, Leibniz Institute of Photonic Technology, Albert
            Einstein Strasse 9, Jena 07745, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021899715.1-RS_2024_03_17
            Annotation Date                   :: 03/17/2024 03:35:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,224
            CDSs (total)                      :: 4,128
            Genes (coding)                    :: 4,018
            CDSs (with protein)               :: 4,018
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 60 of 110
            Pseudo Genes (incomplete)         :: 47 of 110
            Pseudo Genes (internal stop)      :: 13 of 110
            Pseudo Genes (multiple problems)  :: 9 of 110
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091597.1.
            Bacteria and source DNA available from Sascha D. Braun.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11378
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="Nord4-2"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pR32_3"
                     /geo_loc_name="Germany"
                     /collection_date="2018-02-18"
     gene            complement(join(11323..11378,1..1522))
                     /locus_tag="L5465_RS20490"
                     /old_locus_tag="L5465_20490"
     CDS             complement(join(11323..11378,1..1522))
                     /locus_tag="L5465_RS20490"
                     /old_locus_tag="L5465_20490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_237414282.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIIILLKHWIKY
                     LFLKLRDY"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1512..1919)
                     /locus_tag="L5465_RS20495"
                     /old_locus_tag="L5465_20495"
     CDS             complement(1512..1919)
                     /locus_tag="L5465_RS20495"
                     /old_locus_tag="L5465_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2922..3608
                     /locus_tag="L5465_RS20500"
                     /old_locus_tag="L5465_20500"
     CDS             2922..3608
                     /locus_tag="L5465_RS20500"
                     /old_locus_tag="L5465_20500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            3699..4232
                     /gene="aadB"
                     /locus_tag="L5465_RS20505"
                     /old_locus_tag="L5465_20505"
     CDS             3699..4232
                     /gene="aadB"
                     /locus_tag="L5465_RS20505"
                     /old_locus_tag="L5465_20505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(4391..5059)
                     /locus_tag="L5465_RS20510"
                     /old_locus_tag="L5465_20510"
     CDS             complement(4391..5059)
                     /locus_tag="L5465_RS20510"
                     /old_locus_tag="L5465_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     gene            complement(5192..5677)
                     /locus_tag="L5465_RS20515"
                     /old_locus_tag="L5465_20515"
     CDS             complement(5192..5677)
                     /locus_tag="L5465_RS20515"
                     /old_locus_tag="L5465_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            complement(5786..7600)
                     /locus_tag="L5465_RS20520"
                     /old_locus_tag="L5465_20520"
     CDS             complement(5786..7600)
                     /locus_tag="L5465_RS20520"
                     /old_locus_tag="L5465_20520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(7590..7997)
                     /locus_tag="L5465_RS20525"
                     /old_locus_tag="L5465_20525"
     CDS             complement(7590..7997)
                     /locus_tag="L5465_RS20525"
                     /old_locus_tag="L5465_20525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            9000..9686
                     /locus_tag="L5465_RS20530"
                     /old_locus_tag="L5465_20530"
     CDS             9000..9686
                     /locus_tag="L5465_RS20530"
                     /old_locus_tag="L5465_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            9777..10310
                     /gene="aadB"
                     /locus_tag="L5465_RS20535"
                     /old_locus_tag="L5465_20535"
     CDS             9777..10310
                     /gene="aadB"
                     /locus_tag="L5465_RS20535"
                     /old_locus_tag="L5465_20535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(10469..11137)
                     /locus_tag="L5465_RS20540"
                     /old_locus_tag="L5465_20540"
     CDS             complement(10469..11137)
                     /locus_tag="L5465_RS20540"
                     /old_locus_tag="L5465_20540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     ARG             9778..10307
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             3700..4229
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             9766..10310
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     ARG             3688..4232
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          complement(3..1522)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5790..7600)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgatttctgt gcttgctgta agctttgctg atcgctcttg attgtctttg attgtgctga
       61 caaggcttgg ataattttgg tttgcattgt gtctaaagct tgctgttgct tctgcaattg
      121 cacttgttgt tgcattaatt gttgctgttg ttcttcgatg attctcaata attgtgtttc
      181 taattcggtc atcgtttaat accccattgg aatcatgtag ccgttctgcc attcgcctgc
      241 gttcttgttg atctgaatac ataggcgctg tgctttgctg tctggtcgct tgcatgtgct
      301 gatcgtcaaa ctgcctcccg atcggttcaa atggtcgacc ctcatttgct tctcgttgat
      361 cacggtattt agcgtctgca tctcgctgta cttgtttttg gtgacgagac caagcgagaa
      421 aattgctata agcaaaataa ctgctactga aatcagtccc atattcaaag tggtaagttt
      481 tttctgtacc gcttgattct gggcttgttg ttcttggctc tgtatttgct cgtttaattg
      541 cgttaaatgg ctcaatttcg attgcagcaa ggtttgatac aggcttaact gcttctcgac
      601 cgtctgggcg ttgttttgaa tgctctgggc tattttctcg tccgactgtt ggatagcggt
      661 cgcagtgata ttggctctta tgttcaagct gtctctgata gtctgtgacg tttgttcgat
      721 accgctcaga agttgttcct ctgtattctt caattcgttg ttgtacttct tgtctatatt
      781 cgccagtagc tctaaagcct tgctcataaa tctcgccctc taaacggatg ggacgttttg
      841 cgccctcata tgggttttca atggatatgg agttttttac ttgtcgagtg acattgattt
      901 gattcgattc aagccattgc accaattctt gccgatcggc aacatcacca ttggcaaccg
      961 ctcggtaaac acgttgatgt aactgcgctt taaaatcctt aatatctttg ggtaggttct
     1021 ttttatttaa aaatagctgt cgatgctcag gatcatcggg gtcatacagt gaatgttcgg
     1081 tattagtgat ctgcttaaat agatcgactc gatctaaatc gactggcgca tagaatggct
     1141 gtaggcgttt gcctgttgaa agctcaacat taggaatcac aaaattaagc tcaagccttg
     1201 tctcacctgt gtcctgatta attttgtctt ggtgctgtac ccataaaatt tgatattggt
     1261 ctttatctag tcccgggaat aaacattcct caaaattttg catgattttc tgtttatcct
     1321 ggtcagataa atcatgctca taaaaggaca aacaaccact ggtatatttt ttggcaaaag
     1381 gactgctatc aatgagttgt gctgtcagtt ctacatcacc attgagcact tgagcatgtt
     1441 cacgctctcg atcttcacct aacaaataat cgagacagcc ttttgataaa cctgaaccat
     1501 gtctaaagaa atcaacgatc atctggacgt aactcccgaa gctgctgatc tatggaaatc
     1561 aacaaatgaa gtaacttcac tttgtcaaaa gtacctaccc ctgcaatatc ggtattcact
     1621 gctttagcaa tctgattcac attgttgcca atccgaccaa gctctaaaat taatgctcgc
     1681 tctgtcttgc tatacgaaga ttcaacaacc tttgttgctt taattttttg gtcatcttct
     1741 gctttggaat tatgaatttt agtcgctttc tctactgctg cttgtctaag caactttgca
     1801 atcgaaccat aaggattatt ggaatcgagc aattgatatt ctgattctga aagtctgact
     1861 ttaaaggttc gttctcgaac tgctttgctc tgtttgtttt cttgttcctg tcctgacatt
     1921 tcaatctacc ctgctttggc ttttgtaagc tttaaattgg aaaatctttg atttttctgt
     1981 ttaaagcttt tcgggggtat cgggggtgaa gcccctgatc aaggtcacag actatcactg
     2041 tttaacgaag ttaaataggg gtagctgtgt atccttgctt atttaaaaat ggacgagtga
     2101 aattcaataa aaactaatca aatgacacgg tatataaact ctagtaggta ttggcatgaa
     2161 tcttgtaaaa acaaatgcgc tcaactcttt ctcaccgtcc ccacatccct cgctttgctc
     2221 gggctgatgg tgaacgttga gagagtttcg gcaatacttt ccctaagagg ctttgattta
     2281 aaaaaatcaa atatcctctt aagtattgcc gattaacctg taattgggct gacggagcca
     2341 cgcatcggtc gtatccgcac atggttttgc tgaccatgca gacaaggact aaaggttttg
     2401 caccttctcc cacgcgctcc ctgtcgaagt atggtggtat ctctgcgagg attactccaa
     2461 catgcaactt aaccaaccta tacaactact tccaacgctc cgcctatgtg agatcccctg
     2521 taagcggttt agccttgcga ctatcgctac gatgcaccag tgacacttat tccctccaca
     2581 tgcaacgcat atcttcctta aaaaaggcgc acagaagtag ataaagctcg accgttaggt
     2641 gcttatttga tgatttaaac tgctgaatta acgaaggact tcaaattgag gcttgtcaaa
     2701 ctgttctaat tctttaaaat agatagtggt actagccttt aaggtgaata tcagtatgag
     2761 ttgcaagctc tgccgatctt tcgttaattg atgttgcaag catcaaataa ctgtacccat
     2821 cgaatttaaa actaatggac agatttcgca agagacctgt ccattatttt ttttgattac
     2881 acctaagaat taagctgaaa ctcctattct ttggactatt aatgtcaaaa cttcctagat
     2941 ttcataacaa gcacatcgaa aaacgtaagt attcaaatag cctcctagcc aaatctattt
     3001 cttctaaagt acgtttctct aataaaacat tttttaatgt taatttgaga ggatcccgcc
     3061 tacgatcatg ttcattcaaa aatataaaaa ttgttttatc tgatttttca ggagtaatct
     3121 taaataagaa taggtttacg aatgttcact tcaaaaagtg cgtattttat gctgctattt
     3181 ttagagattg tcgctttaaa aattgcacat ttgaaaaatg catttttatt aatacaaata
     3241 cagaaagcct aaatcactcg atattagact gttctaaaaa atataatttt tacgaaatga
     3301 aaattcctga tgacttaaaa gatcaactaa cagaatatag aaatattcca atactgcaaa
     3361 aaaaccgttt acttcatcta aaagggggaa gaatcaatac cgcaacctta ttcattctcc
     3421 tatccaaaat atccaaatac aagctgctaa atggtcttaa aaaaatttat attgaggaac
     3481 aaagtaaagt aaaaatactt agcactttca aactccttga agaaataaag aacctagttg
     3541 acactaacac cgcattcaaa cagaataatg gtgctctgcc ccatccaatc gaaaggttgg
     3601 atagttaaga caatcactgg gagctcacta ttggatgtaa caattcaatt aatagtctaa
     3661 ttactgacat tctgggaggg cttactatgt taggccgcat ggacacaacg caggtcgcat
     3721 tgatacacca aattctagct gcggcagatg agcgaaatct gccgctctgg atcggtgggg
     3781 gctgggcgat cgatgcacgg ctagggcgtg taacacgcaa gcacgatgat attgatctga
     3841 cttttcccgg cgagaggcgc ggcgagctcg aggcaatagt tgaaatgctc ggcgggcgcg
     3901 tcacggagga gttggactat ggattcttag cggagatcgg ggatgagtta cttgactgcg
     3961 aacctgcttg gtgggcagac gaagcgtatg aaatcgcgga ggctccgcag ggctcgtgcc
     4021 cagaggcggc tgagggtgtc atcgccgggc ggccagtccg ttgtaacagc tgggaggcga
     4081 tcatctggga ttacttttac tatgccgatg aagtaccacc agtggactgg cctacaaagc
     4141 acatagagtc ctacaggttc gcatgcacct cactcggggc ggaaaaggtt gaggtcttgc
     4201 gtgccgcttt caggtcgcga tatgcggcct aacaattcgt ccaagccgac gccgctttgc
     4261 ggcgcggctt aactcaggtg ttaggaaaca ctgctaccgt tatcggattt actgctttac
     4321 tactaacctt gttaattcaa catttgcaaa atagtctgac ctttcgattg tttggggtga
     4381 aattcttatc ttaaaaatct aaatattctt ttagatctaa tgcagattgc tcgataaaat
     4441 cgtatgtatt aatacaacga cagtaaataa attttgttaa aacctctaaa tcattttcta
     4501 gattacggtt aaaaataatc ggttcatgat gagcaatcct gtttcttaat tcacgtaatt
     4561 gatctgtcca atcgtaaaaa atttgcctgt tttcaactgt tgcattagga aaaatagaat
     4621 taaaacatcc ctgccaaata cgttcttcat aattagccat aagcatcttt tgccagaata
     4681 caaatttaag ctcagggatc attttactag gcgaatctgg gaacttctct ttagcactat
     4741 caaacatact tttagggcta tacctccctc tacggctaag gcttcgaata aaagcttcat
     4801 tttcatgcca actatttgtt tcattacacc taaaagatat ttctattgcc tgaacaacag
     4861 catttctcag aacaacttca aaataattta ataagtaaaa aaacttattg gaaattatta
     4921 aattacgttc ataaagcatc aatacatcag atttttcata tggttcacct ttatctttat
     4981 agaaattttc atacttagat aagcgtggta tagataaagc ctgctctatc ttaagcactg
     5041 catcatttga caattccacc gtatataccc taatatatag ttgtaagttt gagagactgc
     5101 ggatctaaga tcttccctcc caagcgctaa gattcaaaaa agcctttcat taacgtgaaa
     5161 ggcttttttc tttttttggt caattaaaag cttacatatt gattgtaatt gaaatatttt
     5221 cttgagggac atcaaaataa ttagctaata gtctcttagc ttcaggaata gatattttat
     5281 ccagtgtttt agaagaatta tcttcattta atttggtaaa tttcagctca ttcaaatcga
     5341 tattcatctc ttctaatgaa ctataagaaa ctggattccg attaccatcc cacatattgg
     5401 gaatagatat atcagcatat tcggagatat ttattatcca tctaccatct gcatcttctt
     5461 cttgagattg gctgatacta gatattttgc caactaaaaa tgcagtgtga tgatcagcct
     5521 ctggttctcc ccaagattgt ttcctatttt gcacacaaac tagataatcg cactttcctg
     5581 cattatgagc atttagtctc caagactgac taccaccttc ttttaaaatt ctagtctttc
     5641 cacgagcagt tagtactact acacagttat tattcataac accaccaaaa tatataaaca
     5701 agcctatact acattcaata tagctaaatt taaacattag tacaagctaa tactaagaaa
     5761 taaacaccaa catactagaa aataattaca tcccaaaatc catacctcga tcttgcttag
     5821 gtcgaggttt ttctatctct tgtttacgct cattcatctg gacgagattt ttttcaatat
     5881 ctcggatgtt tcgagcaaat tctgctgcgc tttggctact tctgacgaga cagaatgctg
     5941 tgtggttgag attctctcca gaatttcttc gttgttggct gtgataaggc tcatctctcg
     6001 gactttgcca ttgatctgct cggtatagct tgctgtactt cgttctagct cgtgaaattg
     6061 cttctgcatt gtcgctaatg atttctgtgc ttgctgtaag ctttgctgat cgctcttgat
     6121 tgtctttgat tgtgctgaca aggcttggat aattttggtt tgcattgtgt ctaaagcttg
     6181 ctgttgcttc tgcaattgca cttgttgttg cattaattgt tgctgttgtt cttcgatgat
     6241 tctcaataat tgtgtttcta attcggtcat cgtttaatac cccattggaa tcatgtagcc
     6301 gttctgccat tcgcctgcgt tcttgttgat ctgaatacat aggcgctgtg ctttgctgtc
     6361 tggtcgcttg catgtgctga tcgtcaaact gcctcccgat cggttcaaat ggtcgaccct
     6421 catttgcttc tcgttgatca cggtatttag cgtctgcatc tcgctgtact tgtttttggt
     6481 gacgagacca agcgagaaaa ttgctataag caaaataact gctactgaaa tcagtcccat
     6541 attcaaagtg gtaagttttt tctgtaccgc ttgattctgg gcttgttgtt cttggctctg
     6601 tatttgctcg tttaattgcg ttaaatggct caatttcgat tgcagcaagg tttgatacag
     6661 gcttaactgc ttctcgaccg tctgggcgtt gttttgaatg ctctgggcta ttttctcgtc
     6721 cgactgttgg atagcggtcg cagtgatatt ggctcttatg ttcaagctgt ctctgatagt
     6781 ctgtgacgtt tgttcgatac cgctcagaag ttgttcctct gtattcttca attcgttgtt
     6841 gtacttcttg tctatattcg ccagtagctc taaagccttg ctcataaatc tcgccctcta
     6901 aacggatggg acgttttgcg ccctcatatg ggttttcaat ggatatggag ttttttactt
     6961 gtcgagtgac attgatttga ttcgattcaa gccattgcac caattcttgc cgatcggcaa
     7021 catcaccatt ggcaaccgct cggtaaacac gttgatgtaa ctgcgcttta aaatccttaa
     7081 tatctttggg taggttcttt ttatttaaaa atagctgtcg atgctcagga tcatcggggt
     7141 catacagtga atgttcggta ttagtgatct gcttaaatag atcgactcga tctaaatcga
     7201 ctggcgcata gaatggctgt aggcgtttgc ctgttgaaag ctcaacatta ggaatcacaa
     7261 aattaagctc aagccttgtc tcacctgtgt cctgattaat tttgtcttgg tgctgtaccc
     7321 ataaaatttg atattggtct ttatctagtc ccgggaataa acattcctca aaattttgca
     7381 tgattttctg tttatcctgg tcagataaat catgctcata aaaggacaaa caaccactgg
     7441 tatatttttt ggcaaaagga ctgctatcaa tgagttgtgc tgtcagttct acatcaccat
     7501 tgagcacttg agcatgttca cgctctcgat cttcacctaa caaataatcg agacagcctt
     7561 ttgataaacc tgaaccatgt ctaaagaaat caacgatcat ctggacgtaa ctcccgaagc
     7621 tgctgatcta tggaaatcaa caaatgaagt aacttcactt tgtcaaaagt acctacccct
     7681 gcaatatcgg tattcactgc tttagcaatc tgattcacat tgttgccaat ccgaccaagc
     7741 tctaaaatta atgctcgctc tgtcttgcta tacgaagatt caacaacctt tgttgcttta
     7801 attttttggt catcttctgc tttggaatta tgaattttag tcgctttctc tactgctgct
     7861 tgtctaagca actttgcaat cgaaccataa ggattattgg aatcgagcaa ttgatattct
     7921 gattctgaaa gtctgacttt aaaggttcgt tctcgaactg ctttgctctg tttgttttct
     7981 tgttcctgtc ctgacatttc aatctaccct gctttggctt ttgtaagctt taaattggaa
     8041 aatctttgat ttttctgttt aaagcttttc gggggtatcg ggggtgaagc ccctgatcaa
     8101 ggtcacagac tatcactgtt taacgaagtt aaataggggt agctgtgtat ccttgcttat
     8161 ttaaaaatgg acgagtgaaa ttcaataaaa actaatcaaa tgacacggta tataaactct
     8221 agtaggtatt ggcatgaatc ttgtaaaaac aaatgcgctc aactctttct caccgtcccc
     8281 acatccctcg ctttgctcgg gctgatggtg aacgttgaga gagtttcggc aatactttcc
     8341 ctaagaggct ttgatttaaa aaaatcaaat atcctcttaa gtattgccga ttaacctgta
     8401 attgggctga cggagccacg catcggtcgt atccgcacat ggttttgctg accatgcaga
     8461 caaggactaa aggttttgca ccttctccca cgcgctccct gtcgaagtat ggtggtatct
     8521 ctgcgaggat tactccaaca tgcaacttaa ccaacctata caactacttc caacgctccg
     8581 cctatgtgag atcccctgta agcggtttag ccttgcgact atcgctacga tgcaccagtg
     8641 acacttattc cctccacatg caacgcatat cttccttaaa aaaggcgcac agaagtagat
     8701 aaagctcgac cgttaggtgc ttatttgatg atttaaactg ctgaattaac gaaggacttc
     8761 aaattgaggc ttgtcaaact gttctaattc tttaaaatag atagtggtac tagcctttaa
     8821 ggtgaatatc agtatgagtt gcaagctctg ccgatctttc gttaattgat gttgcaagca
     8881 tcaaataact gtacccatcg aatttaaaac taatggacag atttcgcaag agacctgtcc
     8941 attatttttt ttgattacac ctaagaatta agctgaaact cctattcttt ggactattaa
     9001 tgtcaaaact tcctagattt cataacaagc acatcgaaaa acgtaagtat tcaaatagcc
     9061 tcctagccaa atctatttct tctaaagtac gtttctctaa taaaacattt tttaatgtta
     9121 atttgagagg atcccgccta cgatcatgtt cattcaaaaa tataaaaatt gttttatctg
     9181 atttttcagg agtaatctta aataagaata ggtttacgaa tgttcacttc aaaaagtgcg
     9241 tattttatgc tgctattttt agagattgtc gctttaaaaa ttgcacattt gaaaaatgca
     9301 tttttattaa tacaaataca gaaagcctaa atcactcgat attagactgt tctaaaaaat
     9361 ataattttta cgaaatgaaa attcctgatg acttaaaaga tcaactaaca gaatatagaa
     9421 atattccaat actgcaaaaa aaccgtttac ttcatctaaa agggggaaga atcaataccg
     9481 caaccttatt cattctccta tccaaaatat ccaaatacaa gctgctaaat ggtcttaaaa
     9541 aaatttatat tgaggaacaa agtaaagtaa aaatacttag cactttcaaa ctccttgaag
     9601 aaataaagaa cctagttgac actaacaccg cattcaaaca gaataatggt gctctgcccc
     9661 atccaatcga aaggttggat agttaagaca atcactggga gctcactatt ggatgtaaca
     9721 attcaattaa tagtctaatt actgacattc tgggagggct tactatgtta ggccgcatgg
     9781 acacaacgca ggtcgcattg atacaccaaa ttctagctgc ggcagatgag cgaaatctgc
     9841 cgctctggat cggtgggggc tgggcgatcg atgcacggct agggcgtgta acacgcaagc
     9901 acgatgatat tgatctgact tttcccggcg agaggcgcgg cgagctcgag gcaatagttg
     9961 aaatgctcgg cgggcgcgtc acggaggagt tggactatgg attcttagcg gagatcgggg
    10021 atgagttact tgactgcgaa cctgcttggt gggcagacga agcgtatgaa atcgcggagg
    10081 ctccgcaggg ctcgtgccca gaggcggctg agggtgtcat cgccgggcgg ccagtccgtt
    10141 gtaacagctg ggaggcgatc atctgggatt acttttacta tgccgatgaa gtaccaccag
    10201 tggactggcc tacaaagcac atagagtcct acaggttcgc atgcacctca ctcggggcgg
    10261 aaaaggttga ggtcttgcgt gccgctttca ggtcgcgata tgcggcctaa caattcgtcc
    10321 aagccgacgc cgctttgcgg cgcggcttaa ctcaggtgtt aggaaacact gctaccgtta
    10381 tcggatttac tgctttacta ctaaccttgt taattcaaca tttgcaaaat agtctgacct
    10441 ttcgattgtt tggggtgaaa ttcttatctt aaaaatctaa atattctttt agatctaatg
    10501 cagattgctc gataaaatcg tatgtattaa tacaacgaca gtaaataaat tttgttaaaa
    10561 cctctaaatc attttctaga ttacggttaa aaataatcgg ttcatgatga gcaatcctgt
    10621 ttcttaattc acgtaattga tctgtccaat cgtaaaaaat ttgcctgttt tcaactgttg
    10681 cattaggaaa aatagaatta aaacatccct gccaaatacg ttcttcataa ttagccataa
    10741 gcatcttttg ccagaataca aatttaagct cagggatcat tttactaggc gaatctggga
    10801 acttctcttt agcactatca aacatacttt tagggctata cctccctcta cggctaaggc
    10861 ttcgaataaa agcttcattt tcatgccaac tatttgtttc attacaccta aaagatattt
    10921 ctattgcctg aacaacagca tttctcagaa caacttcaaa ataatttaat aagtaaaaaa
    10981 acttattgga aattattaaa ttacgttcat aaagcatcaa tacatcagat ttttcatatg
    11041 gttcaccttt atctttatag aaattttcat acttagataa gcgtggtata gataaagcct
    11101 gctctatctt aagcactgca tcatttgaca attccaccgt atatacccta atatatagtt
    11161 gtaagtttga gagactgcgg atctaagatc ttccctccca agcgctaaga ttcaaaaaag
    11221 cctttcatta acgtgaaagg cttttttctt tttttggtca attaaaagct tacatattga
    11281 ttgtaattga aatattttct tgagggacat caaaataatt agctaatagt ctcttagctt
    11341 caggaataga tattttatcc agtgttttag aagaatta
//
