LOCUS       NZ_CP091537             2182 bp    DNA     circular CON 15-MAR-2024
DEFINITION  Escherichia coli strain 537-20 plasmid p537-20_3, complete sequence.
ACCESSION   NZ_CP091537
VERSION     NZ_CP091537.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25247371
            Assembly: GCF_021869725.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Neumann,B., Lippmann,N., Wendt,S., Karlas,T., Lubbert,C., Werner,G.,
            Pfeifer,Y. and Schuster,C.F.
  TITLE     Recurrent bacteremia with a hypermucoviscous Escherichia coli
            isolated from a patient with perihilar cholangiocarcinoma: insights
            from a comprehensive genome-based analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Neumann,B., Lippmann,N., Wendt,S., Karlas,T., Lubbert,C., Werner,G.,
            Pfeifer,Y. and Schuster,C.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2022) Unit 13: Nosocomial Pathogens and Antibiotic
            Resistances, Robert Koch Institute, Burgstr. 37, Wernigerode 38855,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: Unicycler v. 0.4.9b
            Assembly Name                     :: RKI_Ecoli_537-20
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021869725.1-RS_2024_03_15
            Annotation Date                   :: 03/15/2024 05:57:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,967
            CDSs (total)                      :: 4,844
            Genes (coding)                    :: 4,616
            CDSs (with protein)               :: 4,616
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 115 of 228
            Pseudo Genes (incomplete)         :: 120 of 228
            Pseudo Genes (internal stop)      :: 44 of 228
            Pseudo Genes (multiple problems)  :: 47 of 228
            Pseudo Genes (short protein)      :: 4 of 228
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091537.1.
            Bacteria and source DNA available from Robert Koch Institute, FG13,
            Nosocomial Pathogens and Antibiotic Resistances, Burgstr. 37,
            Wernigerode, Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="537-20"
                     /isolation_source="blood"
                     /host="host"
                     /db_xref="taxon:562"
                     /plasmid="p537-20_3"
                     /geo_loc_name="Germany"
                     /lat_lon="51.3397 N 12.3731 E"
                     /collection_date="2020"
                     /collected_by="Uni Klinik Leipzig"
     gene            123..548
                     /gene="mobA"
                     /locus_tag="L6D13_RS24675"
                     /old_locus_tag="L6D13_24675"
     CDS             123..548
                     /gene="mobA"
                     /locus_tag="L6D13_RS24675"
                     /old_locus_tag="L6D13_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_064261972.1"
                     /translation="MVKNKRSGSETRQRSKVITLRINNKIDREIRRLAKAEGMTISEYI
                     RTSCLNKEIIQRIPSRYLDELLELGRMQKNLFDKGRTPKDKDYLDVMHKIITLCAEAKE
                     VILRNSDIYKEMDLLKQEIKIIKQYYKNKYPGSDLFN"
                     /name="mobA"
     gene            complement(590..1117)
                     /locus_tag="L6D13_RS24680"
                     /old_locus_tag="L6D13_24680"
     CDS             complement(590..1117)
                     /locus_tag="L6D13_RS24680"
                     /old_locus_tag="L6D13_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279566.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_072650454.1"
                     /translation="MSVERYKSNPFLENMIVPVKGRQVKLSRLGKDNNILMNQSTGEFL
                     GTHVTTYKQVDADQFIKIFTDNIAMTFDFTSAGIKAFGVLLWAVQHHALSKDEVDLDSF
                     ILDAFISENNKENKKIRLSMATFKRGINELEKASIIAKTLRKGRYYINPNFVFNGDRIA
                     FTNLIERKAKDK"
                     /name="replication/maintenance protein RepL"
ORIGIN
        1 tgtagcgaaa aacgggacag gatgtgtatt tgtcataccg cgaatacatt cgcttgtatg
       61 aaaatacctc ctgtcagggc aggccccgac accccgaacg tggaaaccag aggttcattg
      121 tgatggttaa aaataagcgc agcggctcag aaacacggca gagaagcaag gttataacct
      181 tgcggataaa taacaagatt gacagggaga tccgacgcct ggcaaaggcg gaagggatga
      241 cgatttcaga atatataaga acgtcatgtt taaataaaga aataatacag cgcatccctt
      301 ccagatattt ggatgaatta ttagagcttg gccggatgca gaagaattta tttgataaag
      361 gaagaacacc gaaggataag gactaccttg atgtgatgca caaaataatc acgctgtgcg
      421 cagaagcgaa ggaggttatt ttacgtaatt ctgatatata taaagagatg gatttattaa
      481 aacaggaaat aaaaataatt aaacagtact acaaaaataa atacccaggc tctgatttat
      541 ttaattaaaa taaaccctgc tcaattttta gagcggggtt tatatgttgt tatttgtctt
      601 ttgcttttct ttctatcaga tttgtgaagg ctattctatc accattaaaa acaaagtttg
      661 ggtttatata ataacgtcct tttcttaggg tttttgctat tatacttgct ttctcaagtt
      721 cattaatgcc ccttttaaat gtggccatac tcaaccttat cttcttgttt tctttattat
      781 tttcagagat gaaggcatcc aatataaaac tatctaaatc aacttcatct ttagataatg
      841 catgatgctg aaccgcccaa agaaggacac caaaagcttt aattccggcg gaagtaaaat
      901 caaatgtcat cgctatatta tctgtaaata ttttgataaa ttgatcggcg tcgacttgtt
      961 tatatgttgt cacgtgagta ccaaggaact ctcccgtcga ttgattcatt aatatattat
     1021 tatctttgcc taaacgagat aatttaacct gcctaccttt gacaggcacg atcatatttt
     1081 ccaaaaatgg gttcgatttg tacctttcaa cgctcataat gcaccattat attgaacctt
     1141 agtagctaaa aaagcgccct ttcaaagctc ataatgaacg aactatttga gctttgaaag
     1201 ctcacactat agctaacacg gctcaaaaac aacacctatt ttgagccgtg ttggtaacag
     1261 ggttgagcta acaagttaca gatccgaaga tcacgatccc catttcatgc gggttgcaga
     1321 ggattccctt cttagttctt atacggtggt gcgtaagtgc cctaggctga catctggttc
     1381 acttccgctt cctcgcccac tcgctcacta acgttcggtc gctggggctg cggcgagcgg
     1441 atcctcctct ctcgtagtgg ctggcttttg gtgttgttct ttcgaagatg agtcgtcagc
     1501 atggccctaa aacgcgctca gagcctctca aacgcacgaa acggggttta aatgcccgtg
     1561 agggcatgga aggcgcgaaa aacgcttaca gagggtttca ggaggccagg cgcgccgata
     1621 cccctaacta ggggctttca ggtcgctagg aaccgcgcaa gccccctgca aagcccaacc
     1681 gcacgggaca ggccgccact cggttggtgg gtggtcgaag accgttggtg ttacagcggc
     1741 gggggtctgg gggtctcccc agacggtttc cggtagcgct caaggcgctg tcggaaccgt
     1801 ggcgggtttt ttttaacaaa aaacgacagg tcggaaatta aagcgcgaac tgaacttata
     1861 tttcacatat ataaacgcga tagttatcat gcacacacat atatattcgc ttcatcgttg
     1921 taatctgtgc gatttctgcc ttaatcgagc atattgacgg catcggttaa cactgaaaat
     1981 aatactatcg cgaggattaa ataattattt agcatatata taccgcagac gataagagaa
     2041 gaaacgtaat atcaaataaa aataataaaa aaaataacaa gcaccatttt aaaacctgtg
     2101 taaaaaatat tgcgtgatgc cttgcttttt tgtattgaag ataccagctt atgaggtagt
     2161 tttttatttt ccgccgaaaa gt
//
