LOCUS       NZ_CP091536             6647 bp    DNA     circular CON 15-MAR-2024
DEFINITION  Escherichia coli strain 537-20 plasmid p537-20_2, complete sequence.
ACCESSION   NZ_CP091536
VERSION     NZ_CP091536.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25247371
            Assembly: GCF_021869725.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Neumann,B., Lippmann,N., Wendt,S., Karlas,T., Lubbert,C., Werner,G.,
            Pfeifer,Y. and Schuster,C.F.
  TITLE     Recurrent bacteremia with a hypermucoviscous Escherichia coli
            isolated from a patient with perihilar cholangiocarcinoma: insights
            from a comprehensive genome-based analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Neumann,B., Lippmann,N., Wendt,S., Karlas,T., Lubbert,C., Werner,G.,
            Pfeifer,Y. and Schuster,C.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2022) Unit 13: Nosocomial Pathogens and Antibiotic
            Resistances, Robert Koch Institute, Burgstr. 37, Wernigerode 38855,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: Unicycler v. 0.4.9b
            Assembly Name                     :: RKI_Ecoli_537-20
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021869725.1-RS_2024_03_15
            Annotation Date                   :: 03/15/2024 05:57:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,967
            CDSs (total)                      :: 4,844
            Genes (coding)                    :: 4,616
            CDSs (with protein)               :: 4,616
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 115 of 228
            Pseudo Genes (incomplete)         :: 120 of 228
            Pseudo Genes (internal stop)      :: 44 of 228
            Pseudo Genes (multiple problems)  :: 47 of 228
            Pseudo Genes (short protein)      :: 4 of 228
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091536.1.
            Bacteria and source DNA available from Robert Koch Institute, FG13,
            Nosocomial Pathogens and Antibiotic Resistances, Burgstr. 37,
            Wernigerode, Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="537-20"
                     /isolation_source="blood"
                     /host="host"
                     /db_xref="taxon:562"
                     /plasmid="p537-20_2"
                     /geo_loc_name="Germany"
                     /lat_lon="51.3397 N 12.3731 E"
                     /collection_date="2020"
                     /collected_by="Uni Klinik Leipzig"
     gene            complement(join(5769..6647,1..690))
                     /gene="cea"
                     /locus_tag="L6D13_RS24625"
                     /old_locus_tag="L6D13_24625"
     CDS             complement(join(5769..6647,1..690))
                     /gene="cea"
                     /locus_tag="L6D13_RS24625"
                     /old_locus_tag="L6D13_24625"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            950..1387
                     /locus_tag="L6D13_RS24630"
                     /old_locus_tag="L6D13_24630"
     CDS             950..1387
                     /locus_tag="L6D13_RS24630"
                     /old_locus_tag="L6D13_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            1468..1887
                     /locus_tag="L6D13_RS24635"
                     /old_locus_tag="L6D13_24635"
     CDS             1468..1887
                     /locus_tag="L6D13_RS24635"
                     /old_locus_tag="L6D13_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2205..2438)
                     /locus_tag="L6D13_RS24640"
                     /old_locus_tag="L6D13_24640"
     CDS             complement(2205..2438)
                     /locus_tag="L6D13_RS24640"
                     /old_locus_tag="L6D13_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2445..2963)
                     /locus_tag="L6D13_RS24645"
                     /old_locus_tag="L6D13_24645"
     CDS             complement(2445..2963)
                     /locus_tag="L6D13_RS24645"
                     /old_locus_tag="L6D13_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3001..3699)
                     /locus_tag="L6D13_RS24945"
                     /pseudo=""
     CDS             complement(<3001..3699)
                     /locus_tag="L6D13_RS24945"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3641..3964)
                     /locus_tag="L6D13_RS24655"
                     /old_locus_tag="L6D13_24655"
     CDS             complement(3641..3964)
                     /locus_tag="L6D13_RS24655"
                     /old_locus_tag="L6D13_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4028..4219
                     /locus_tag="L6D13_RS24660"
                     /old_locus_tag="L6D13_24660"
     CDS             4028..4219
                     /locus_tag="L6D13_RS24660"
                     /old_locus_tag="L6D13_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4889..5047
                     /locus_tag="L6D13_RS24950"
     CDS             4889..5047
                     /locus_tag="L6D13_RS24950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(5246..5383)
                     /locus_tag="L6D13_RS24665"
                     /old_locus_tag="L6D13_24665"
     CDS             complement(5246..5383)
                     /locus_tag="L6D13_RS24665"
                     /old_locus_tag="L6D13_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            5545..5772
                     /locus_tag="L6D13_RS24670"
                     /old_locus_tag="L6D13_24670"
     CDS             5545..5772
                     /locus_tag="L6D13_RS24670"
                     /old_locus_tag="L6D13_24670"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     Typing          complement(4305..4392)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          4403..4961
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.544"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2102..3651)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 catttctgca tcacgggcat ggatactgga gcttaaacga gaattgagag tcttaatctc
       61 tccatccatt ttcaccactt cagattgtgc agcagaaagt tttttttggg cgatctcaac
      121 agctttagct tcttcactca atgcagccag tcgtttctct tcagcttcag ccagtttcaa
      181 ctggcgttct gtttcagcct tctcccgttc aatctcttta cgtcgttgtt ctgcttcctg
      241 aaaagccttt tctgctgctt ccgcttcttt acgggctttt tcttctgctt tcgcaaggcg
      301 caaacgctct gcttccgcct gcatagctgc attattagca tgagcaagct ctgttgctga
      361 aggcgtacgt gaggcattgt gacgaagagc ctcattcacg atatccttca ggcgctgagt
      421 cagcgcatcc ctgtttgcct ttgctttcgc ctgtgcttcc gctgcagctt ttgcccgggc
      481 agcctgctct gcctgtgttt tctttaattg agcagtagac catttagcag ttgcatgaat
      541 agctgcagaa ctttcacttt tactgcctcc ttttccacct ccgccgccag agccactccc
      601 gtcaggagta ccattcaaaa gagtaataat tacctgtccc ttatcatcat aaggaacacc
      661 atctttatag tacgctaccg cggtttccat tataaaatcc tctttgactt ttaaaacaat
      721 aagttaaaaa taaatactgt acatataacc actggtttta tatacagcat aaaagctacg
      781 ccgctgcatt ttccctgtca agactgtgga cttccatttt tgtgaaaacg atcaaaaaaa
      841 cagtctttca caccacgcgc tattctcgcc cgatgccaca aaaaccagca caaacattac
      901 cgttctcaga cctcattatg ttttactgaa actatgagat gagacatcta tgggacactg
      961 tcactttatg gcatggcaca cactccggga cgcactaaaa atgacaggca gatcgcgttc
     1021 acagttttac cgtgatatgc gcgcaggcct tgtcagttac cgtaccggca gggacggacg
     1081 acgggagttt gaaaccagtg aactgatccg ggcatacggc gaattaaagc agaatgagac
     1141 accagaaagg cacagtgagg gacatgcaga aaatccacat gatcagcaga cagaacgcat
     1201 tctccgggag ctgaatgagc tgaaacaatg cctgacgctg atgcttgagg ataaacaggc
     1261 acaggatatg gatcgcagac gccaggaagc agaacgggaa cagctacaaa atgagatagc
     1321 ccagctcagg caggcactgg aactggaaaa gaaacgggga ttctggtcca ggttgttcgg
     1381 tcgctgaacg ctgtcagaga ctgatgataa aatagtcttc ggataataac tcaccgagaa
     1441 taaatacttt aaggtaggga gacactcatg agacgtaccg gaaacaaact ttgtcttatc
     1501 gccatgataa cagcaacagt agctctcaca gcctgtaccc caaagggcag cgtggaacaa
     1561 catacccggc attacgtata tgcttctgat gacggttttg atcccaactt ttccacccaa
     1621 aaagccgaca caacacgaat gatggtgcct ttttttcggc agttctggga tatgggagct
     1681 aaagataaag cgacaggaaa atcacggagt gatgtgcaac aacgcattca gcagtttcac
     1741 agccaggaat ttttaaactc actccggggc acaactcaat ttgcgggtac tgattaccgc
     1801 agcaaagacc ttaccccgaa aaaatccagg ctgctggctg acacgatttc tgcggtttat
     1861 ctcgatggct acgagggcag acagtaagtg gatttaccat aatcccttaa ttgtacgcac
     1921 cgctaaaacg cgttcagcgc gatcacggca gcagacaggt aaaaatggca acaaaccacc
     1981 cgaaaaactg ccgcgatcgc gcctgataaa ttttaaccgt atgaatacct atgcaaccag
     2041 agggtacagg ccacattacc cccacttaat ccactgaagc tgccattttt catggtttca
     2101 ccatcccagc gaagggccat ccagcgtgcg ttcctgtatt tccggctgac gctcccgttc
     2161 cagggataac acatgttcgc gctcctgtat cagccgttcc tctcttatct ccagttctcg
     2221 ctgtataact ggctcaagcg ttctgtctgc tcgctcaagt gctgcacctg ctgactcaac
     2281 tgcatgaccc gctcgttcag catcgcgttg tcccgttgcg taagcgaaaa catcttctgc
     2341 aattccacga aggcgctctc ccattcgctc agccgctgca tatagtcctg ttgcagctgc
     2401 tctaaggcgt tcagcaaatg tgtttccagc tctgtcactc tgtgtcactc cttcagatgt
     2461 acccactctt tcccctgaaa gggaatcacc tccgctgatt tcccgtacgg aaggacaagg
     2521 aatttcctgt tcccgtcctg cacaaactcc acgccccatg tcttcgcgtt cagtttctgc
     2581 aatgtctctt cctgcttcct gatttcttcc aggttcgcct gtatcctccc tccaagatac
     2641 cagagcgtcc cgccactcgc ggtaaacagg agaaagacta tccccagtaa catcatgccc
     2701 gtattccctg ccagctttaa cacgtccctc ctgtgctgca tcatcgcctc tttcacccct
     2761 tcccggtgtt tttccagcga ttcctctgtc gaggctgtga acagggctat agcgtctctg
     2821 attttcgtct cgtttgatgt cacagcctcg cttacagatt cgccgagcct cctgaactcg
     2881 ttgttcagca ttttctctgt agattcggct ctctctttca gctttttctc gaactccgcg
     2941 cccgtctgca aaagattgct cataaaatgc tcctttcagc ctgatattct tcccgccgtt
     3001 cggatctgca atgctgatac tgcttcgcgt caccctgacc acttccagcc ccgcctcagt
     3061 gagcgcctga atcacatcct gacggccttt tatctctccg gcatggtaaa gtgcatctat
     3121 acctcgcgtg acgccctcag caagcgcctg tttcgtttca ggcaggttat cagggagtgt
     3181 cagcgtcctg cggttctccg gggcgttcgg gtcatgcagc ccgtaatggt gattcaccag
     3241 cgtctgccag gcatcaattc taggcctgtc tgcgcggtcg tagtacggct ggaggcgttt
     3301 tccggtctgt agctccatgt tcggaatgac aaaattcagc tcaagccgtc ccttgtcctg
     3361 gtgctccacc cacaggatgc tgtactgatt tttttcgaga ccgggcatca gtacacgctc
     3421 aaagctcgcc atcacttttt cacgtcctcc cggcggcagc tccttctccg cgaacgacag
     3481 aacaccggac gtgtatttct tcgcaaatgg cgtggcatcg atgagttccc ggacttcttc
     3541 cggattaccc tgaagcaccg ttgcgccttc gcggttacgc tccctcccca gcaggtaatc
     3601 aaccggacca ctgccaccac cttttcccct ggcatgaaat ttaactatca tcccgcgccc
     3661 cctgttccct gacagccaga cgcagccggc gcagctcatc cccgatggcc atcagtgcgg
     3721 ccaccacctg aacccggtca ccggaagacc actgcccgct gttcacctta cgggctgtct
     3781 gattcaggtt atttccgatg gcggccagct gacgcagtaa cggcggtgcc agtgtcggca
     3841 gttttccgga acgggcaacc ggctccccca ggcagacccg ccgcatccat accgccagtt
     3901 gtttaccctc acagcgttca agtaaccggg catgttcatc atcagtaacc cgtattgtga
     3961 gcatcctctc gcgtttcatc ggtatcatta ccccatgaac agaaatcccc cttacacgga
     4021 ggcatcagtg actaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca
     4081 gacattaacg ctgctggaga agctcaacga actggacgca gatgaacagg ccgatatttg
     4141 tgaatcgctt cacgaccacg ccgatgagct ttaccgcagc tgcctcgcac gtttcgggga
     4201 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtgagc
     4261 ggatgccggg agctgacaag cccgtcaggg cgcgtcagca ggttttagcg ggtgtcgggg
     4321 cgcagccctg acccagtcac gtagcgatag cggagtgtat actggcttaa ccatgcggca
     4381 tcagtgcgga ttgtatgaaa agtacgccat gccgggtgtg aaatgccgca cagatgcgta
     4441 aggagaaaat gcacgtccag gcgcttttcc gcttcctcgc tcactgactc gctacgctcg
     4501 gtcgttcgac tgcggcgagc ggtactgact cacacaaaaa cggtaacaca gttatccaca
     4561 gaatcagggg ataaggccgg aaagaacatg tgagcaaaag accaggaaca ggaagaaggc
     4621 cacgtagcag gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatagacg
     4681 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4741 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4801 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgttggtatc tcagttcggt
     4861 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4921 cgccttatcc ggtaactatc gtcttgagtc caacccggta aggcacgcct taacgccact
     4981 ggcagcagcc actggtaacc ggattagcag agcgatgatg gcacaaacgg tgctacagag
     5041 ttcttgaagt agtggcccga ctacggctac actagaagga cagtatttgg tatctgcgct
     5101 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5161 accgttggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5221 tctcaagaag atcctttgat cttttctact gaaccgcgat ccccgtcagt ttagaagagg
     5281 aggatggtgc gatggtccct ccctgaacat cacgtatata gttagcctga catccaacaa
     5341 ggaggtttat cgcgaatatt cccacaaaaa atcttttcct cataactcga tccttataaa
     5401 atgaaaagaa tatatggcga ggtttaattt atgagcttaa gatactacat aaaaaatatt
     5461 ttatttggcc tgtactgcac acttatatat atatacctta taacaaaaaa cagcgaaggg
     5521 tattatttcc ttgtgtcaga taagatgcta tatgcaatag tgataagcac tattctatgt
     5581 ccatattcaa aatatgctat tgaatacata gcttttaact tcataaagaa agattttttc
     5641 gaaagaagaa aaaacctaaa taacgccccc gtagcaaaat taaacctatt tatgctatat
     5701 aatctacttt gtttggtcct agcaatccca tttggattgc taggactttt tatatcaata
     5761 aagaataatt aaatccctaa cacctcattt atagtattaa gtttattctt atcaatatag
     5821 gagcatagaa ttcctgtaac aatagcaata ccccaaatac ctaatgtagt tccagcaagc
     5881 aagctaaaaa gtaaagcaac aacataactc acccctgcat ctgcagcttt cttctctaat
     5941 gtaagaaata gtggcttcca gtcacctgta tccttaattt ttaggatatc agataccaca
     6001 tcatatccaa aagaaacatg ccccgtaatc ttcaagtact tggcaaactg atctaaatgt
     6061 ttagcccagt catcatactt caccgatgcc aacgcattaa aaatagcatc acgatcggct
     6121 ttgctgaatt tcttatttaa aacatccttg tatttttcaa aagcagcgag agcttcattc
     6181 acattgccga ttttcttacc tttagactta tcagcaagtt cctgtgccat tttcgaatat
     6241 ttttcaccat atttttcagt cagcgtttga taaaagctaa ctgttgcatc aacagcatcc
     6301 ttaatctgtg aattaaggag attattctgt gcttttttca aattttcttc agcttcatga
     6361 acacgagcga taccggcatt acgattatta ctgacctgag aaatagcctt ctggatctga
     6421 gttatatcag catttatccg gttaatacgt gtttctgatg ctgttacctg tttttgtttt
     6481 tcttctctaa tcttaccggc cccaacccgt cgtctggttg cttcaaaaaa aggacggttc
     6541 tgaagcggat cattggctct tggtgatagt tttttgacca gctcatccag ttctttatat
     6601 ttagcggatg cctgagccag ttcatttcgt tttccagcga gcgtttt
//
