LOCUS       NZ_CP091524             2483 bp    DNA     circular CON 11-JUN-2024
DEFINITION  Staphylococcus aureus strain N12HSA28 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP091524
VERSION     NZ_CP091524.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25263541
            Assembly: GCF_023373745.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Chen,Y., Ji,S., Sun,L., Wang,H., Zhu,F., Chen,M., Zhuang,H.,
            Wang,Z., Jiang,S., Yu,Y. and Chen,Y.
  TITLE     The novel fosfomycin resistance gene fosY is present on a genomic
            island in CC1 methicillin-resistant Staphylococcus aureus
  JOURNAL   Emerg Microbes Infect 11 (1), 1166-1173 (2022)
   PUBMED   35332834
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Chen,Y. and Chen,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2022) Infectious Diseases, Zhejiang University, 3
            East Qingchun Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023373745.1-RS_2024_06_11
            Annotation Date                   :: 06/11/2024 04:17:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 40 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 16 of 67
            Pseudo Genes (multiple problems)  :: 18 of 67
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091524.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="N12HSA28"
                     /isolation_source="secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed1"
                     /geo_loc_name="China"
                     /lat_lon="30 N 104 E"
                     /collection_date="2016-01"
     gene            join(2456..2483,1..707)
                     /gene="erm(C)"
                     /locus_tag="L5471_RS14000"
                     /old_locus_tag="L5471_14000"
     ARG             4..704
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="95.9"
     gene            1155..1631
                     /locus_tag="L5471_RS14005"
                     /old_locus_tag="L5471_14005"
     CDS             1155..1631
                     /locus_tag="L5471_RS14005"
                     /old_locus_tag="L5471_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000664727.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     gene            2336..2395
                     /gene="ermCL"
                     /locus_tag="L5471_RS14010"
                     /old_locus_tag="L5471_14010"
     CDS             2336..2395
                     /gene="ermCL"
                     /locus_tag="L5471_RS14010"
                     /old_locus_tag="L5471_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="WP_002497446.1"
                     /translation="MGIFSIFVISTVHYQPNKK"
                     /name="ermCL"
     ARG             4..707
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.57"
                     /coverage="95.9"
     Typing          1156..1641
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaactttat tacttcaaaa cataatatag ataaaataat gacaaatata agattaaatg
       61 aacatgataa tatctttgaa atcggctcag gaaaagggca ttttaccctt gaattagtac
      121 agaggtgtaa tttcgtaact gccattgaaa tagaccataa attatgcaaa actacagaaa
      181 ataaacttgt tgatcacgat aatttccaag ttttaaacaa ggatatattg cagtttaaat
      241 ttcctaaaaa ccaatcctat aaaatatttg gtaatatacc ttataacata agtacggata
      301 taatacgcaa aattgttttt gatagtatag ctgatgagat ttatttaatc gtggaatacg
      361 ggtttgctaa aagattatta aatacaaaac gctcattggc attattttta atggcagaag
      421 ttgatatttc tatattaagt atggttccaa gagaatattt tcatcctaaa cctaaagtga
      481 atagctcact tatcagatta aatagaaaaa aatcaagaat atcacacaaa gataaacaga
      541 agtataatta tttcgttatg aaatgggtta acaaagaata caagaaaata tttacaaaaa
      601 atcaatttaa caattcctta aaacatgcag gaattgacga tttaaacaat attagctttg
      661 aacaattctt atctcttttc aatagctata aattatttaa taagtaagtt aagggatgca
      721 gtttatgcat cccttaactt gtttttcgtg tacctatttt ttgtgaatcg attatgtctt
      781 ttgcgcattc acttcttttc tatataaata tgagcgaagc gaataagcgt cggaaaagca
      841 gcaaaaagtt tcctttttgc tgttggagca tgggggttca gggggtgcag tatctgacgt
      901 caatgccgag cgaaagcgag ccgaagggta gcatttacgt tagataaccc cctgatatgc
      961 tccgacgctt tatatagaaa agaagattca actaggtaaa atcttaatat aggttgagat
     1021 gataaggttt ataaggaatt tgtttgttct aatttttcac tcattttgtt ctaatttctt
     1081 ttaacaaatg ttcttttttt tttagaacag ttatgatata gttagaatag tttaaaataa
     1141 ggagtgagaa aaagatgaaa gaaagatatg gaacagtcta taaaggctct cagaggctca
     1201 tagacgaaga aagtggagaa gtcatagagg tagacaagtt ataccgtaaa caaacgtctg
     1261 gtaacttcgt aaaggcatat atagtgcaat taataagtat gttagatatg attggcggaa
     1321 aaaaacttaa aatcgttaac tatatcctag ataatgtcca cttaagtaac aatacaatga
     1381 tagctacaac aagagaaata gcaaaagcta caggaacaag tctacaaaca gtaataacaa
     1441 cacttaaaat cttagaagaa ggaaatatta taaaaagaaa aactggagta ttaatgttaa
     1501 accctgaact actaatgaga ggcgacgacc aaaaacaaaa atacctctta ctcgaatttg
     1561 ggaactttga gcaagaggca aatgaaaaac aagaaaatgc attatctgat tattattctt
     1621 tcaaggacta gtataacata aaatcgtcta caaatagaca aaaaacctgc acgcttaatg
     1681 tagatcaaaa gcttaacgca aatgaaatag attgacctcc caataacacc acgtagttat
     1741 tgggaggtca atctatgaaa tgcgattaag ctttttctaa ttcacataag cgtgcaggtt
     1801 taaagtacat aaaaaatata atgaaaaaaa gcatcattat actaacgtta taccaacatt
     1861 atactctcat tatactaatt gcttattcca atttcctatt ggttggaacc aacaggcgtt
     1921 agtgtgttgt tgagttggta ctttcatggg attaatccca tgaaaccccc aaccaactcg
     1981 ccaaagcttt ggctaacaca cacgccattc caaccaatag ttttctcggc ataaagccat
     2041 gcaaagccat gctctgacgc ttaaatgcac taatgcctta aaaaaacatt aaagtctaac
     2101 acactagact tatttacttc gtaattaagt cgttaaaccg tgtgctatac gaccaaaagt
     2161 ataaaacctt taagaacttt cttttttctt gtaaaaaaag aaactagata aatctctcat
     2221 atcttttatt caataatcgc atcagattgc agtataaatt taacgatcac tcatcatgtt
     2281 catatttatc agagctcgtg ctataattat actaatttta taaggaggaa aaaatatggg
     2341 catttttagt atttttgtaa tcagcacagt tcattatcaa ccaaacaaaa aataagtggt
     2401 tataatgaat cgttaataag caaaattcat tataaccaaa ttaaagaggg ttataatgaa
     2461 cgagaaaaat ataaaacaca gtc
//
