LOCUS       NZ_CP091501             3709 bp    DNA     circular CON 09-AUG-2023
DEFINITION  Enterobacter cloacae complex sp. ECL404 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP091501
VERSION     NZ_CP091501.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25233125
            Assembly: GCF_021869625.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae complex sp. ECL404
  ORGANISM  Enterobacter cloacae complex sp. ECL404
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Zhang,X.
  TITLE     The study of the whole genome of Enterobacter cloacae complex
            carrying mcr-9 is helpful to describe the possible transmission mode
            of mcr-9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Zhang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2022) Department of Microbiology, Yongchuan
            Hospital of Chongqing Medical University, Xuanhua Road, Yongchuan,
            Chongqing 402106, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 2.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/07/2023 04:47:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,209
            Genes (coding)                    :: 5,026
            CDSs (with protein)               :: 5,026
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 77 of 183
            Pseudo Genes (incomplete)         :: 104 of 183
            Pseudo Genes (internal stop)      :: 21 of 183
            Pseudo Genes (multiple problems)  :: 18 of 183
            Pseudo Genes (short protein)      :: 1 of 183
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091501.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Enterobacter cloacae complex sp. ECL404"
                     /mol_type="genomic DNA"
                     /strain="ECL404"
                     /isolation_source="Drainage"
                     /db_xref="taxon:2912631"
                     /plasmid="unnamed5"
                     /geo_loc_name="China: Chongqing"
                     /collection_date="2020-10-05"
     gene            complement(join(3595..3709,1..102))
                     /locus_tag="L3071_RS26555"
                     /old_locus_tag="L3071_26555"
                     /pseudo=""
     CDS             complement(join(3595..3709,1..102))
                     /locus_tag="L3071_RS26555"
                     /old_locus_tag="L3071_26555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(109..600)
                     /locus_tag="L3071_RS26560"
                     /old_locus_tag="L3071_26560"
     CDS             complement(109..600)
                     /locus_tag="L3071_RS26560"
                     /old_locus_tag="L3071_26560"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_136070821.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESRDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<635..1069)
                     /locus_tag="L3071_RS26565"
                     /old_locus_tag="L3071_26565"
                     /pseudo=""
     CDS             complement(<635..1069)
                     /locus_tag="L3071_RS26565"
                     /old_locus_tag="L3071_26565"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1278..1601)
                     /locus_tag="L3071_RS26570"
                     /old_locus_tag="L3071_26570"
     CDS             complement(1278..1601)
                     /locus_tag="L3071_RS26570"
                     /old_locus_tag="L3071_26570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_163063187.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(1717..1890)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.714"
                     /coverage="100.0"
                     /evalue="1.2e-79"
     Typing          1814..2420
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.803"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3..1288)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.271"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1285..1601)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.41e-59"
ORIGIN
        1 ccgccattcc gcgaaggcgc tctcccattc gtccagcctt tgcgagtagt cctgctgtag
       61 ctgctctaat gcgctcagca actgcttttc cagctctgtc attttccgct actcctgttt
      121 caggcggatc catgtcgtcc cgtcgtaggg gatggcttcg gtttgcatcc cgggcggcag
      181 gataagaaac ctccggccgt cgcggctctc ctgatagcgc actccccacg tccgggcagt
      241 cattttcgcc agggtatctt cctgctgacg aagatgcgtg taattgtcgg ttatctgctg
      301 cccctgccac cacagtatgc tcccgctcgt gccgatcagc agcgctgaca ccatcaggat
      361 ggtgaaccat gtccgcccgg cagtccgcag tacgctccgc cggtttttct ccatcgcctc
      421 gctcatactc tgctcgtgtg cgaggatggc gtcgctgatt ctcctcgcgc ttgcgctcag
      481 ttccgctctg acagactgct cgtgctcgct gaatgcggct ttcagcatct cgccggtact
      541 ctgcttctgc gctttcgatt gctgctctaa gtccttcgcc agcgttaaaa gactgctcat
      601 agatggctcc cttcagtcgg atgtttcgcc ccccgtccgg gtcggcaatg ctgatactgc
      661 ttttcgtggt gcgcaccacc tcaaaacctg accgttccag cgcctcagtg acgtcctgac
      721 ggtttttcag ctccccggac gaagcaaggg tcagcagtcc gcgcgtgatg gcttcagtgg
      781 cctcctgctt cgttttcggc agtccggacg gcgtgaccag cgcccggcgg ttttccggcg
      841 cgttcgggtc atgcagcccc agtctgccgt tcacgatggt ctgccaggca tcgatgcgcg
      901 gccggtcggc gcggtcgtag tacggctgga gccgcctgcc ggtcagcagt tcggtattgg
      961 ggatcagaaa gttcagctcc agacgcccct tgtccgcgtg ctccacccac agaatgctgt
     1021 actgatcttt atcgagtccg ggcatcagaa cccgctcgaa gctggccatc agtttttccc
     1081 gctggccggg aggcagatcg gcctccgcaa acgacagtac gccggaggtg tatttcttaa
     1141 cgtaaggcga cgcgtcaatc agctcccgga cttcttccgg cttcccctgc aggacgctgg
     1201 cgccttcacg ctgccggtct ttacccagca gatagtccac cggcccggcg ccaccgccgc
     1261 gaccgcgtgg atgaaattta acgatcatcg tccgttcctt tctcaagaac ggcgtgccgc
     1321 agccgctcaa gcccggcatc gatggccatc agcgccgcca caatctgtac cctgtcatgg
     1381 ccggtgccgc cgccggcgtt aacacggcgg gctatctggt tcaggttatt gcccataccg
     1441 gcaagctggc gcagcagcgc cggcgagagt gaggggagct ttccggtgcg cgcaggcttt
     1501 tcgtccaggc aggtctgccg catccacgcc gccagcggcc tgccgtcgca gcgctcaagc
     1561 agccgccggt gctcgccctc cgtcacccac atggtgagca tcttgttccg tttctctgac
     1621 aaaccggcct cctgataacg ccgggacggg cgggaaagcc tttcccggtc ggcgcggggt
     1681 tggacaagcc gcaggcgcgt cagtggcttt tggcgggttt cggggcgcag ccctgaacca
     1741 gtcacgtagc gctagcggag tgtatactgg cttactatgt tggcacgaat gcggatgtca
     1801 gtgaagtgct tcatgtggca ggaggaaaaa ggcggcaccg gtgcgtcagc agaatatgtg
     1861 atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggaa atggcttacg aacagggcgg aaatttcctg gaagatgcca ggaagaaact
     1981 taacagggaa gcgataaggc cacgccagag ccgtttttcc ataggctccg cccccctgac
     2041 aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg acttaaagat
     2101 accaggcgtt tccccctggt ggctccctcg tgcgctctcc tgttcctgcc tttcggttta
     2161 ccggtgtcac tccgctgtta tggccgcgtt tatctcattc cacgcctgac actcggttcc
     2221 gggaaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgactgc
     2281 tgcgcctgtt ccggtaacta tcgacttgag tccaagccgg aaagacacga caaagcgcca
     2341 ctggcagcag ccactggtaa gagggtgtac aagagattac tgatgcagag ttcttgaagt
     2401 cagagccaaa ctacggctaa actggaagga agaatttggt ggcagcggtc ttgtacaccc
     2461 tgttaccacg gttcagaagt tccccaactt actgaacctt cgaaaaacca cctccccagg
     2521 tggttttttc gttttcagag caagagatta cgacgcaacg gaaaggatct caagaggttc
     2581 atcttcgatg accgacagac atcaaagtaa aaccgatacc aaacaacagt accagtcaaa
     2641 aactcacacc gacaaaaccc tttgaaaata aagcataaaa aatcgtatag ttggatgatg
     2701 cacaatcacc caacaaacca gcataaccat ttgaataaat agaaaaataa aaatatagga
     2761 tattagagta atgaaataca tttgctttgc agcagtaatg atttccctta caggatgcgt
     2821 taattcaagt ggcgtattac ccatggggcc agatacttat tccatatctg ttcatgctgc
     2881 acctgtaagg ggacaggctg gagcaaaaaa agctgcgtta acagaagcga accaatattg
     2941 ccttaatcaa ggcaagacca tccttgtgaa gaacatggat cactctacgt ttagctcgct
     3001 cccaggggat gatataagca tcatattcca atgcttatcg gttggcgata gtggattaca
     3061 gcgacctaat tatcaaagtc ctgctgatgt aaacgtcaac gttaattagg caccatttac
     3121 cataacatcc gttgggcgca ccgctgaaac ggggttggct gcgtttccgg tgccagacgc
     3181 gagaaagtgg cgacagacat aacgaaaaaa caccagaaac gcagccgctg aatattacac
     3241 tggtgcatag tcatgcagcc agatgcatgg atgttaaagt cacactaaaa tccgcgccaa
     3301 tgccttaaaa accgacaaat gcttctccag ccagtcccac agctccgccg ccagatacag
     3361 gcagttaagc aatgattcaa taagatttaa cagcttcatt tcccgctcct gaataagtcg
     3421 tgaccttttt gatcacgaac ctatttagcg ggagaaataa gagcccctct tcgtgcgggt
     3481 tgtcagctgc ctggatggtt taaagtgtcg gcccgcggta ggttttctgc ggctggcgct
     3541 ccagctccag tgttttctga tactcattct ccctctgccg gagctcgatg gcattcagtt
     3601 cccggacaag cggcgcagct gctcgcttaa actcagcacc tgcgtgctca agtccgacac
     3661 gcgctcgttc agcaccgcgt tctcccgttg cataagccca cacatgctc
//
