LOCUS       NZ_CP091484             4716 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Enterobacter cloacae complex sp. ECL414 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP091484
VERSION     NZ_CP091484.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25233122
            Assembly: GCF_021869685.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae complex sp. ECL414
  ORGANISM  Enterobacter cloacae complex sp. ECL414
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Zhang,X.
  TITLE     The study of the whole genome of Enterobacter cloacae complex
            carrying mcr-9 is helpful to describe the possible transmission mode
            of mcr-9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Zhang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2022) Department of Microbiology, Yongchuan
            Hospital of Chongqing Medical University, Xuanhua Road, Yongchuan,
            Chongqing 402106, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021869685.1-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 04:30:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,067
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,876
            CDSs (with protein)               :: 4,876
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 20 of 76
            Pseudo Genes (incomplete)         :: 53 of 76
            Pseudo Genes (internal stop)      :: 11 of 76
            Pseudo Genes (multiple problems)  :: 7 of 76
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091484.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Enterobacter cloacae complex sp. ECL414"
                     /mol_type="genomic DNA"
                     /strain="ECL414"
                     /isolation_source="sputum"
                     /db_xref="taxon:2912628"
                     /plasmid="unnamed3"
                     /geo_loc_name="China: Chongqing"
                     /collection_date="2021-10-13"
     gene            complement(164..379)
                     /locus_tag="L3068_RS25215"
                     /old_locus_tag="L3068_25215"
     CDS             complement(164..379)
                     /locus_tag="L3068_RS25215"
                     /old_locus_tag="L3068_25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(386..877)
                     /locus_tag="L3068_RS25220"
                     /old_locus_tag="L3068_25220"
     CDS             complement(386..877)
                     /locus_tag="L3068_RS25220"
                     /old_locus_tag="L3068_25220"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_064138460.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTARRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGIQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<912..1616)
                     /locus_tag="L3068_RS25370"
                     /pseudo=""
     CDS             complement(<912..1616)
                     /locus_tag="L3068_RS25370"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1555..1878)
                     /locus_tag="L3068_RS25230"
                     /old_locus_tag="L3068_25230"
     CDS             complement(1555..1878)
                     /locus_tag="L3068_RS25230"
                     /old_locus_tag="L3068_25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_236904887.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2529..2657
                     /locus_tag="L3068_RS25375"
     CDS             2529..2657
                     /locus_tag="L3068_RS25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_074185011.1"
                     /translation="MHEPPVQSDCCALSGNYRLESKPERHDKAPLAAAIGNWSRWI"
                     /name="replication initiation protein"
     gene            2883..3320
                     /locus_tag="L3068_RS25235"
                     /old_locus_tag="L3068_25235"
     CDS             2883..3320
                     /locus_tag="L3068_RS25235"
                     /old_locus_tag="L3068_25235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010787163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapD"
                     /protein_id="WP_064138458.1"
                     /translation="MSKYLITFDLDTTKLKVLYHGNNYTNAYYDIRSTLSRRGFENIQG
                     SVYLGEEGVSEAHGTLAIQELTAKYSWFYDCVSNIRFYRIESDLNAQFIADGVNEAKNR
                     FYAQLEDMKKSLISVGLSVEQANDVIGQQQFKLENMMPAKK"
                     /name="VapD"
     gene            3789..4394
                     /locus_tag="L3068_RS25240"
                     /old_locus_tag="L3068_25240"
     CDS             3789..4394
                     /locus_tag="L3068_RS25240"
                     /old_locus_tag="L3068_25240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_235836678.1"
                     /translation="MNNKRFARLPNWWLRSEDPEDLLLVRLKSGNTGTGIAAMKCLLAL
                     SVLIDFHSRVAEVSLTGLETLTGLSRPMVIRGLSKLEDLGVVNSDKSFRTNRYELTIKK
                     DDTYWAKVPVEIVRKKLKTISNRGIAPFTALKLYITILGLRYKEKTTVQVMHDTLRDYT
                     GIQTTQVRYGLDVLYSSGLIHLMPREDRSTNEYQVIGL"
                     /name="replication protein"
     Typing          complement(1994..2167)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          2091..2638
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.196"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(73..1565)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.8"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcacgaacc tatttagcgg gagaaataag agcccctctt cgtgcgggtt gtcagctgcc
       61 tggatggttt aaagtgtcgg cccgcggtag gttttctgcg gctggcgctc cagctccagt
      121 gttttctgat actcattctc cctctgccgg agctcgatgg cattcagttc ccggacaagc
      181 ggcgcagctg ctcgcttaaa ctcagcacct gcgtgctcaa gtccgacacg cgctcgttca
      241 gcaccgcgtt ctcccgttgc ataagcccac acatgctccg ccattccgcg aaggcgctct
      301 cccattcgtc cagcctttgc gagtagtcct gctgtagctg ctctaatgcg ctcagcaact
      361 gcttttccag ctctgtcatt ttccgctact cctgtttcag gcggatccat gtcgtcccgt
      421 cgtaagggat ggcttcggtt tgtatccccg gcggcaggat aagaaacctc cggccgtcgc
      481 tgctctcctg atagcgcact ccccacgtcc gggcggtcat tttcgccagg gtatcttcct
      541 gctgacgaag atgcgtgtaa ttgtcggtta tctgctgccc ctgccaccac agaatgctcc
      601 cgctcgtgcc gatcagcagc gctgacacca tcaggatggt gagccatgtc cgcctggcag
      661 tccgcagtac gctccgccgg tttttctcca tcgcctcgct catactctgc tcgtgggcga
      721 ggatggcgtc gctgattctc ctcgcgcttg cgctcagttc cgctctgaca gactgctcgt
      781 gctcgctgaa tgcggctttc agcatctcgc cggtactctg ctgctgcgct ttcgattgct
      841 gctctaagtc cttcgccagc gttaaaagac tgctcataga tggctccctt cagtcggatg
      901 tttcgccccc cgtccgggtc ggcaatgctg atactgcttt tcgtggtgcg caccacctca
      961 aaacctgcac cttcaagagt gtcagtgaca tcctgacggc ttttcagctc cccggacgaa
     1021 gcaagggtca gcagtccccg ggtgatggct tctgcggcct cctgcttcgt tttcggcagc
     1081 ccggacggcg tgaccagcgc ccggcggttt tccggcgcgt tcgggtcgtg cagccccagt
     1141 ctgccgttca cgatagtctg ccaggcatcg atgcgcggcc ggtcggcgcg gtcgtagtaa
     1201 ggctggagcc gcctgccggt cagcagctcg gtattgggga tcagaaagtt cagctccaga
     1261 cgccccttgt ccgcgtgctc cacccacaga atgctgtact gatctttatc gagtccgggc
     1321 atcagaaccc gctcgaagct ggccatcagt ttttcccgct ggccgggagg cagatcggcc
     1381 tccgcaaacg acagaacgcc ggaggtgtat ttcttaacgt aaggcgacgc gtcaatcagc
     1441 tcccggactt cttccggctt cccctgcagg acgctggcgc cttcacgctg ccggtcttta
     1501 cccagcagat agtccaccgg cccggcgcca ccgccgcggc cgcgcggatg aaatttaacg
     1561 atcatcgtcc gttcctttct caagaacggc gtgccgcagc cgctcaaggc cggcatcgat
     1621 ggccatcagc gccgccacaa tctgtaccct gtcatggccg gtgccgccgc cggcgttaac
     1681 acggcgggct atctggttca ggttattgcc catgccggca agctggcgca gcagcgccgg
     1741 cgagattgag ggaagctttc cggaacgtgc cggcttttcg tccaggcagg tctgccgcat
     1801 ccacgccgcc agcggcctgc cgtcgcagcg ctcaagcagc cgccggtgct cgccctccgt
     1861 cacccacatg gtgagcatct tgttccgttt ctctgacaaa ccggcctcct gataacgccg
     1921 ggacgggcgg gaaagccttt cccggtcggc gcggggttgg acaagccgca ggcgcgtcag
     1981 tggcttttgg cgggtttcgg ggcgcagccc tgaaccagtc acgtagcgct agcggagtgt
     2041 atactggctt aatatgttgg cacggatacg gaaatcagtg aagtgcttca tgtggcagga
     2101 gaaaaaaggc ggcaacggcg cgtcagcaga atatgcgata caggatatat tccgcttcct
     2161 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtgcgg gcttacgaag
     2221 ggggcggaaa tttcctggta gatgccagga agaaacttaa cagggaaatg ataaggccgc
     2281 ggcgaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc
     2341 aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc cccctggtag
     2401 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
     2461 gccgcgtttg tctcattcca cgcctgacac tcggttccgg gaaggcagtt cgctccaagc
     2521 tggactgtat gcacgaaccc cccgttcagt ccgactgctg cgccttatcc ggtaactatc
     2581 gacttgagtc caagccggaa agacacgaca aagcgccact ggcagcagcc attggtaact
     2641 ggtcaaggtg gatttagata ttgagagtct tgaagtgttg gtctgactac ggctacactg
     2701 aaaggacatt atttggtatc tgagctccac caagccagtt acccggttaa gcagttcccc
     2761 aactgaccta accttcgaaa aaccgcctcc ccaggcggtt ttttcgttta accccggtaa
     2821 gatacgcctt agacttctaa ctcgtcaagc aggccaaaag ccaatggatg caggaacaat
     2881 atatgagcaa gtatctgatt acctttgacc ttgatacgac aaagttaaag gttttgtatc
     2941 acggcaataa ctatacgaat gcttattacg atataagaag cacactaagt cgacgtggtt
     3001 ttgaaaacat acaaggaagc gtctatctag gggaagaggg agtaagtgaa gcccacggca
     3061 cattagcaat tcaagagcta acggcaaagt actcatggtt ttatgattgc gtttctaaca
     3121 tccgatttta tcgaattgaa tctgatttaa atgctcagtt catcgctgac ggggttaacg
     3181 aggcaaaaaa tcgattctat gcccaactgg aagacatgaa gaaatccctc atttccgttg
     3241 gactgtcagt tgaacaagct aacgacgtta tagggcaaca acaattcaaa cttgaaaaca
     3301 tgatgcccgc aaaaaaatag cagatacaac aattgatctg ggagaggctg ttggttatca
     3361 acacctcttc catctttcca tgagccattc cctcctcctc actacacact tcaacacatt
     3421 tactatgagt tcatttattg accactaaaa atgcacatgt ggaagccatc gaacaataaa
     3481 attagttatc tcatttacta ttaaaaaaag ccatttacca acaataaaaa attgatttta
     3541 aacgattttt tataaattta ggattatcgc ttaatctaga tatatatatt aaccccgtgc
     3601 taaaaatttc agtcttgacg tttttcttag gagactgctg tttatggaaa ctctaaacaa
     3661 cgtaatttcc acattagctt cagcgccacc agaagctctg gttgctatcg tttcccttgg
     3721 tgcaatgttc tttatgttta tgataataaa gttatttttc tcatttcttg aacgtaagtg
     3781 gcactctaat gaataacaaa cgctttgcga gacttccaaa ctggtggcta cgttcagaag
     3841 accctgaaga tttactgctg gttagattga aatcaggaaa tacaggtact ggcattgcag
     3901 caatgaagtg tcttctagcc ttgtccgttt tgattgactt tcactctagg gttgcggagg
     3961 tatctttaac tggtttggag acgttaaccg ggcttagtcg ccccatggtt atacgaggat
     4021 tatcaaaact agaggactta ggtgtagtta actcagacaa gagcttcaga acgaatagat
     4081 acgaactaac gatcaaaaaa gatgatacgt actgggcaaa ggtgccagtt gagattgtaa
     4141 gaaaaaagct aaaaacaata tcaaacagag ggatagcacc tttcacggcg ctaaagctgt
     4201 atataacaat attaggcttg aggtataagg agaaaacaac agttcaagta atgcatgata
     4261 ccctcagaga ttacactggc attcagacaa cccaagttag gtatggacta gatgtcttat
     4321 acagcagtgg acttattcac ttgatgccta gagaagatcg gtcaacaaat gaatatcaag
     4381 taataggttt atgattttta tttaccataa catccgttgg gcgcaccgct gaaacggggt
     4441 tggctgcgtt tccggtgcca gacgcgagaa agtggcgaca gacataacga aaaaacacca
     4501 gaaacgcagc cgctgaatat tacactggtg catagtcatg cagccagatg catggatgtt
     4561 aaagtcacac taaaatccgc gccaatgcct taaaaaccga caaatgcttc tccagccagt
     4621 cccacagctc cgccgccaga tacaggcagt taagcaatga ttcaatgaga tttaacagct
     4681 tcatttcccg ctcctgaata agtcgtgacc tttttg
//
