LOCUS       NZ_CP091414             4430 bp    DNA     circular CON 14-MAR-2024
DEFINITION  Staphylococcus aureus strain ATR-20003 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP091414
VERSION     NZ_CP091414.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25143835
            Sequence Read Archive: SRR17682067, SRR17682066
            Assembly: GCF_021725475.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ighem Chi,S., Yousuf,B., Flint,A., Weedmark,K., Pagotto,F. and
            Ramirez-Arcos,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) Process and Products Development, Canadian
            Blood Services, 1575 Carling Ave, Ottawa, ON K1Z 7M3, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JAN-2022
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 591.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021725475.1-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 03:31:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,713
            Genes (coding)                    :: 2,630
            CDSs (with protein)               :: 2,630
            Genes (RNA)                       :: 81
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 43 of 83
            Pseudo Genes (incomplete)         :: 36 of 83
            Pseudo Genes (internal stop)      :: 15 of 83
            Pseudo Genes (multiple problems)  :: 11 of 83
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091414.1.
            Bacteria and source DNA available from Bacteria and source DNA
            available from Dr. Sandra Ramirez-Arcos, 1575 Carling Ave, Ottawa,
            ON.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ATR-20003"
                     /isolation_source="isolated from contaminated platelets"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Canada"
                     /collection_date="2020"
                     /collected_by="Canadian Blood Services (Contact: Sandra
                     Ramirez)"
     gene            complement(join(3055..4430,1..2689))
                     /gene="sdrD"
                     /locus_tag="L3I92_RS13945"
                     /old_locus_tag="L3I92_002781"
     CDS             complement(join(3055..4430,1..2689))
                     /gene="sdrD"
                     /locus_tag="L3I92_RS13945"
                     /old_locus_tag="L3I92_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /GO_process="GO:0007155 - cell adhesion [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrD"
                     /protein_id="WP_237269247.1"
                     /translation="MLNRENKTAMTRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFGI
                     GSQEAKAAEVTNKEMKEDATSVNNDQVSKKVDTGQLNKDGNTSKVDTEQLNDENINKVA
                     NQKEVTRVENSVASEKNNNSQPSENGKLQTSDVKKTEDNNATSDDQVTTTVNSQQLNTD
                     NTTSNATMSAAPINVSKDDLKINSEEVRNVGEKSDNTDNANGSDVIMPKSTAPKRLNTR
                     MRIAAVQPSSTDSKNVNNLITSTTTLTVVDADKNNKIVPAQDYLALKSQIKVDDKVKSG
                     DYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTANNLITYTFTDYVDRF
                     NSVQMGINYSIYMDADTIPVSKNDVEFNVTIGNDTTKTTANIQYPDYVVNEKNSIGSAF
                     TETVSHVGNKENPGYYKQTIYVNPSENSLTNAKLKVQAYHSSYPNNIGQINKEVTDIKI
                     YQVPKGYTLNKGYDVNTKELTDVTNQYLQKITYGDNNSAVIDFGNADSAYVVMVNTKFQ
                     YTTSESPTLVQMVTLSSDNSKSASMGNALGFTNNQSGGAGQEVYKIGNYVWEDTNKNGV
                     QELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPNLPNGDYRVEFSNLPQGYEVT
                     PSKQGNNEELDSNGVSSVITVNGKDNLSADLGIYKPKYNLGDYVWEDTNKNGIQDQDEK
                     GISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGNYKVEFTTPEGYTPTTVTSGSDI
                     EKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNYVWEDTNKDGKQDSTEKGISGVTV
                     TLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEFETPLGYTPTQVGSGTDEGIDSNGT
                     STTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTNKNGVQDKDEKGISGVTVTLKDENDK
                     VLKTVTTDENGKYQFTDLNNGTYKVEFETPSGYTPTSVTSGNDTEKDSNGLTTTGVIKD
                     ADNMTLDSGFYKTPEYSLGDYVWYDSNKDGKQDSTEKGIKDVKVTLLNEKGEVIGTTKT
                     DENGKYRFDNLDSGKYKVIFEKPAGLIQTGTNTTEDDKDADGGEVDVTITDHDDFTIDN
                     GYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSNAGKHTPAKPMSAVKDHNNKAKALPETGTENNNSNNGTLFGGLFAALGSL
                     LLFGRRKKQNK"
                     /name="sdrD"
ORIGIN
        1 ctaagttgta agtcggtttg tagaaaccag agtcaatagt atcgttatct ttatctttaa
       61 tgacaccagt tgttgatgta ccatttgaat ctatgccttc atcagttcct gaacctactt
      121 gtgttggtgt gtaacctaat ggtgtttcga attcaacttt gtatgttcca ttttctaatc
      181 cagtaaattg atatttacca tctttatctg ttttagttgt ttgtaaaact tcaccgtttt
      241 catttttcaa tgtaactgtt acgcctgaaa tacctttttc agttgaatcc tgcttaccat
      301 ctttatttgt atcttcccat acataattac ctaaattata ttttggtgtt ttgtagaatc
      361 cactatctaa tgtcatgtta tcagcaccat taataacacc tgttgttgtt aaaccattag
      421 agtctttttc aatatcgcta ccagatgtta ctgtagtcgg tgtatagcct tctggtgtag
      481 taaattcaac tttataatta ccattatcta aatcagtaaa tttatatttg ccatcagcgt
      541 ctgttgtaac tgtttttaac acgttaccgt tttcatcttt taatgttacc gttacgccag
      601 atataccttt ttcatcttga tcttgaatac catttttatt tgtatcttcc catacatagt
      661 cacctagatt gtatttaggt ttgtaaatac ctaagtcagc agataaatta tctttgccat
      721 taactgttat cactgatgat acaccgtttg aatctaattc ttcgttatta ccttgttttg
      781 aaggagttac ctcataacct tgtggtaaat ttgaaaattc tacacggtaa tctccattag
      841 gtaagtttgg aatcaaatat gacccatctt ctttagtaac tgcttctcct acttttgtat
      901 ttgtattatt atcaaataca gttacagtta cattgccaac gcctttttct cctaattctt
      961 gaacaccgtt tttatttgta tcttcccata cgtagttacc aattttatat acttcttgac
     1021 cagcgccgcc actttggtta ttagtgaatc ctaaagcatt acccattgag gcagatttac
     1081 tattgtctga acttaatgta accatttgaa caagtgttgg gctttcgcta gttgtatatt
     1141 ggaattttgt attaaccatt acaacataag cagaatctgc atttccaaaa tcaataacag
     1201 cgctattgtt gtcgccatat gtaattttct gcaagtattg atttgttaca tctgtaagct
     1261 ctttagtatt cacatcgtat cctttattta atgtataacc tttaggaact tgatatattt
     1321 ttatatctgt tacctcttta tttatttgcc cgatattatt aggataactt gagtggtaag
     1381 cttgaacttt tagtttggca tttgttaaag aattttccga tggatttaca taaatcgttt
     1441 gtttatagta ccccggattt tctttatttc caacatgtga aactgtttca gtgaacgctg
     1501 atccaattga atttttctca tttacaacat aatctggata ttgaatgttt gcagtagtct
     1561 ttgttgtatc attacctatc gtaacattaa actcaacatc gtttttacta acaggaattg
     1621 tatcagcatc catataaatt gaataattaa ttcccatttg tacagaatta aacctatcga
     1681 cataatctgt aaatgtatat gtaattaaat tatttgcagt atcatgtttt gcagtcgcaa
     1741 ttgtttcacc attatttgga tctttaatat caccaatatt tttaatatct tccggattca
     1801 atccatatac ttgtactgta tctgagtatt ttattgtgaa ataatcacct gatttaactt
     1861 tgtcatcaac tttaatttgt gattttaatg ctagataatc ttgtgcaggt acaattttat
     1921 tgtttttatc tgcatcaacg acagttaaag ttgtagttga agtaattaaa ttattaacat
     1981 ttttagagtc tgttgatgat ggttgtactg ctgctatacg cattcttgta ttcaaacgtt
     2041 taggtgctgt actttttggc ataatcacat ctgaaccatt tgcattgtca gtattatcgc
     2101 ttttttctcc tacatttctt acttcttcac tattaatttt taagtcatct ttacttacat
     2161 taattggtgc tgccgacatt gttgcatttg aagttgtgtt atcagtattt aattgctgtg
     2221 aattaactgt agtagtaact tgatcatctg aagttgcatt attatcctca gtttttttaa
     2281 catccgacgt ttgtaatttt ccattttcag atggctgaga gttattattt ttttctgaag
     2341 cgacactatt ttctactcta gttacttctt tttgattagc aactttatta atattctcat
     2401 catttagttg ctctgtatca actttactag tattaccatc cttatttagt tgccctgtat
     2461 caactttttt agacacttga tcattattta ctgatgttgc atcttccttc atttccttgt
     2521 tggttacttc tgcagcttta gcttcttggc tccctatacc aaaaatcaaa gttgtaccaa
     2581 ccagtattga tgctgttccc actgtgtact ttctaatcga aaatttattt aatcgattgg
     2641 ataccatgcc ttttcttgtc attgccgttt tattttctct gtttagcatt agattactcc
     2701 taattcatca aatttttaaa taatacaatt gttttaaata caaaaatgta tatcaatata
     2761 gtattacaat tttagataaa gcacaatact ttaattattt ttctttatcg taaaacttca
     2821 tttaacattt gcgtttaaat aaaagttttt atgagttttg taatctttat ttaatcatca
     2881 taaaaaataa tattatttac ccttaaaatt aatatcttag cttttctaat tcatagacaa
     2941 ttacttttct gtaacaaatt aaattgcatc tattccttaa agatttttgg ttttatatct
     3001 gggaatttct aaacagaaaa accaggccac acggacctgg ttaagctaat catattattt
     3061 gttttgtttt ttacgacgac cgaatagtaa taatgatcct aatgccgcga ataatccacc
     3121 gaataatgtg ccattatttg aattattatt ttcagtacct gtttccggta atgctttcgc
     3181 tttattgtta tggtctttaa ctgcactcat tggtttagcc ggtgtatgtt tacctgcatt
     3241 tgagtcggag tcgctgtctg aatctgaatc gctgtccgaa tctgaatcgc tgtctgagtc
     3301 tgaatcacta tctgaatctg agtcgctatc tgaatctgaa tcgctgtctg aatctgagtc
     3361 gctatctgag tctgaatcgc tgtccgaatc tgaatcgctg tctgagtctg aatcactatc
     3421 tgaatctgaa tcgctatctg agtctgagtc gctgtctgaa tctgagtcgc tatctgaatc
     3481 tgaatcgcta tctgagtctg agtcgctatc tgaatctgag tcgctatctg aatctgagtc
     3541 gctgtctgag tctgaatcac tgtctgagtc tgagtcgcta tctgaatctg aatcgctatc
     3601 tgaatctgaa tcgctgtctg aatctgagtc gctatctgaa tctgagtcgc tatctgagtc
     3661 gctatccgaa tctgaatcac tatctgagtc tgaatcgctg tccgaatctg agtcgctatc
     3721 tgagtctgag tcgctgtctg agtctgaatc actgtctgag tctgaatcgc tatctgatgt
     3781 atcttcttcg aagtaaccgt tgtctattgt aaaatcatca tgatctgtaa ttgttacatc
     3841 tacttctcca ccatcagcat ctttatcatc ttcagttgta tttgtacctg tttgaattaa
     3901 gccagcaggc ttttcaaaaa taactttgta tttaccgcta tctaaattat cgaaacgata
     3961 tttaccattt tcatctgttt tagtagttcc aattacttcg cctttttcat ttaataaagt
     4021 aactttaaca tctttgatac ctttttcagt tgaatcttgt ttaccatctt tattactgtc
     4081 gtaccaaaca taatcaccta aactatattc tggcgttttg tagaaaccac tgtctaatgt
     4141 catgttatct gcatctttaa tgacacctgt tgttgttaaa ccattagaat ctttttcagt
     4201 atcatttcca gaagttactg aagttggtgt ataacctgat ggtgtctcga attcaacttt
     4261 ataagttcca ttgtttaaat cagtaaattg atatttacca ttttcatctg ttgttactgt
     4321 ttttaaaact ttgtcgtttt catcttttaa cgtaacagtt acacctgaaa tgcccttttc
     4381 atctttatct tgaacaccgt ttttatttgt atcttcccat acatagtcac
//
