LOCUS       NZ_CP091325             3958 bp    DNA     circular CON 09-AUG-2023
DEFINITION  Enterobacter sp. JH25 plasmid pDR25-6, complete sequence.
ACCESSION   NZ_CP091325
VERSION     NZ_CP091325.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25126227
            Assembly: GCF_021725435.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. JH25
  ORGANISM  Enterobacter sp. JH25
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Huang,J.
  TITLE     Characterisation of bla NDM-5 and mcr-9 co-occurrence in ST78
            carbapenem-resistant Enterobacter in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Huang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2022) Department of Clinical Laboratory, Jinhua
            Central Hospital, Mingyue street 351, Jinhua, Zhejiang 321000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/09/2023 03:30:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,103
            CDSs (total)                      :: 4,988
            Genes (coding)                    :: 4,891
            CDSs (with protein)               :: 4,891
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 36 of 97
            Pseudo Genes (incomplete)         :: 68 of 97
            Pseudo Genes (internal stop)      :: 13 of 97
            Pseudo Genes (multiple problems)  :: 19 of 97
            Pseudo Genes (short protein)      :: 1 of 97
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091325.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Enterobacter sp. JH25"
                     /mol_type="genomic DNA"
                     /strain="JH25"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:2923088"
                     /plasmid="pDR25-6"
                     /geo_loc_name="China:Jinhua"
                     /collection_date="2018-11-11"
     gene            17..205
                     /locus_tag="L2Y28_RS25480"
     CDS             17..205
                     /locus_tag="L2Y28_RS25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(253..480)
                     /locus_tag="L2Y28_RS25485"
     CDS             complement(253..480)
                     /locus_tag="L2Y28_RS25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224019247.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSYMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            1333..1479
                     /locus_tag="L2Y28_RS25490"
     CDS             1333..1479
                     /locus_tag="L2Y28_RS25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_114456554.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPERHDKAPLAAATGKRVYKRLLM
                     QSS"
                     /name="replication initiation protein"
     gene            complement(1951..2931)
                     /locus_tag="L2Y28_RS25495"
     CDS             complement(1951..2931)
                     /locus_tag="L2Y28_RS25495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="WP_000019450.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGRVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
                     /name="IS5-like element ISKpn26 family transposase"
     gene            3009..3431
                     /locus_tag="L2Y28_RS25500"
     CDS             3009..3431
                     /locus_tag="L2Y28_RS25500"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080052137.1"
                     /translation="MTITYLALIFITLLTINIILKNRRSKKYLAYNICLAQFTYENEST
                     ENRARIENKAKEIIANGSSIQHMKNTIKQNNPLVIYGFYALAMDELGVPPLLYGFDRWY
                     PVRNPFLAEHGLSRHIEKAVDKIENKSKVQWEHWIN"
                     /name="hypothetical protein"
     gene            3454..3744
                     /locus_tag="L2Y28_RS25505"
     CDS             3454..3744
                     /locus_tag="L2Y28_RS25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013813157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="WP_080052136.1"
                     /translation="MKKITIAALSLVLLSGCASVSKSYGPDGNEAYSLTCSGMARDWGM
                     CLTKAGELCGAKGYNILSINGDSGALVTANPQSLMAGSVINRNMLISCKQL"
                     /name="lipoprotein"
     Typing          953..1502
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.918"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 accgggaaaa agccgtatga caaaacagga atcagccgca ctgaacatgg caaaatttat
       61 ccgggcgcag tcgctgctgc tgctcgaaaa actcgacgtg ctcgatcttg atgacgaggc
      121 agccgactgt gagcgaatgc acgaacaggc agaagcgctt tatcagaagc tgagcgcacg
      181 cttcggtata caggacggcg aataaagaga cgaaaagaac gctcacttag acgaaaagcg
      241 tatccatggc aactatgtat atccaccgtg tacatagtca tgcctgacgc tttcgagcag
      301 gtcgagcatg taggaacgac gagcttgatt ggtatcggac acaatatggc gatacgcctc
      361 aaacataaca gcacccgcat caccgcccga acggcgaagc atagcggaaa tcatccgcac
      421 aaagagcaga cgttcatcgg ggcaaaggtt cataagttct cctggtgaaa aaccaaacat
      481 gaagcaccca tctattaacg tcagctgtga acgcttcatt agaagaaaat aggtaaaccc
      541 gggaaaaaga tccaaacgtt aacatcacag gtaaacagaa acgtcgggtc gatcgggaaa
      601 ttctttcccg gacggcgcgg ggttgggcaa gccgcaggcg cgtcagtgct tttagcgggt
      661 gtcggggcgc agccctgaac cagtcacgta gcgctagcgg agtgtatact ggctaaactt
      721 cagagatgcc aaggaaggga caggatatgt tttgtagcac cgccaccacg gtggcgatac
      781 tccaaaacgc ctggtcaggg cacagccccg acaccccaga gccgactgta ggtcactggg
      841 cgaacgcggc ggaatgatgt cggatgacgt cattttgcgg cgtttgccct atcctgcatc
      901 gtattaaaaa atgcagctat ggcgcgtacg gtgccaaaga taagcccgga gatattccgc
      961 ttcctcgctc actgactcgc tccgctcggt cgttcggctg cggcgagcgg taccggctta
     1021 ctggcggggc ggaaatttcc tggcatcttc caggaagaag cttaacaggg aagcaagaag
     1081 gccacggcga agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga
     1141 cgctcaaatc agtggtggcg aaacccgaca ggacttaaag ataccaggcg tttccccctg
     1201 gtggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     1261 tatggccgcg tttatctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     1321 aagctggact gtatgcacga accccccgtt cagtccgact gctgcgcctg ttccggtaac
     1381 tatcgacttg agtccaaccc ggaaagacac gacaaagcgc cactggcagc agccactggt
     1441 aagagggtgt acaagagatt actgatgcag agttcttgaa gtcagagcca aactacggct
     1501 aaactggaag gacgaatttg gtggcagcgg tcttgtacac cctgttacca cggttcagaa
     1561 gttccccaac ttactgaacc ttcgaaaaac cacctcccca ggtggttttt tcgttttcag
     1621 agcaagagat tacgacgcaa cggaaaggat ctcaagaggt tcatcttcga tgaccgacag
     1681 acatcaaagt aaaaccgata ccaaacaaca gtaccagtca aaaactcaca ccgacaaaac
     1741 cctttgaaaa taaagcataa aaaatcgtat agttggatga tgcacaatca ccatttaagc
     1801 tagggaaggt gcgaataagc aggtcatttc ttcccaagct gactcgctga ttaaaatttc
     1861 gcggatctgg gccgattttt ttcccgcaaa cacatcgaat cagcctattt aggctatttt
     1921 ttccaccatt tctggcgtta tttccggttt ttactgagat ctctcccact gacgtatcat
     1981 ttggtccacc cgaaacaggt tggccagggt gaataacatt gccagttggt tatcgttttt
     2041 cagcagcccc ttgtatctgg ctttcacgaa gccgaactgc cgcttgatga tgcgaaacgg
     2101 gtgctccacc ctggcacgga tgctggcttt catgtattcg atgttgatgg ccgttttgtt
     2161 cttgcgcgga tgctgcttca aggtttttac cctgccggga cgctcggcga tcagccagtc
     2221 cacatccacc tcggccagct cctcgcgctg tggcgctcct tggtagccgg catcggctga
     2281 gacaaattgc tcctctccat gaagcagatt acccagctga ttgaggtcat gctcgttggc
     2341 cgcggtggtg accaggctgt gggtcaggcc actcttggca tcgacaccaa tgtgggcctt
     2401 catgccaaag tgccactgat tgcctttctt ggtctgatgc atctccggat cgcgttgctg
     2461 ctctttgttc ttggtagagc tgggtgcctc aatgatggtg gcatccacca aagtgccttg
     2521 ggtcatcatg acgcctgctt cggccagcca gcgattgatg gtcttgaaca attgacgggc
     2581 cagttgatgc tgctcgagca ggtggcggaa attcatgatg gtggtgcgat ccggcagggc
     2641 gctatccagg gataatcggg caaacaggcg catggaggcg atttcgtaca gggcatcttc
     2701 catggcaccg tcgctcaggt tgtaccaatg ctgcatgcag tgaatacgca gcatggtctc
     2761 cagcggatag ggccgtcggc cattgcccgc cttgggataa aacggctcga tgacagcggt
     2821 catattctgc catggcagaa tctgctccat gcgggagagg aaaatctctt ttcgggtctg
     2881 acggcgctta gtgctgaatt cactatcggc gaaggtgagt tgatggctca tgatgtccct
     2941 ctgggatgcg ctccggatga atatgatgat ctcatatcag gaacttgttc gcaccttccc
     3001 tagtaaaaat gacaatcaca tacttagctc ttatatttat caccctacta acaatcaata
     3061 taatattaaa aaatagacgt tctaaaaaat atttagcata taacatctgc cttgctcaat
     3121 tcacttatga aaatgaatct acagaaaaca gagccagaat agaaaataaa gccaaggaaa
     3181 taatagccaa tggttcatcc attcaacaca tgaaaaatac aatcaaacaa aacaatccgc
     3241 ttgtgattta tggcttttac gcattagcaa tggatgagct aggagtcccc cctttactat
     3301 atggatttga taggtggtat ccagttagaa acccattcct tgcagaacat gggctgagtc
     3361 gacatataga aaaggcagta gacaaaatag aaaataaatc aaaagtacaa tgggaacatt
     3421 ggattaatta actttatata aagtaggtgt gaagtgaaaa agataacaat tgcagcatta
     3481 agtcttgtac tactttccgg ctgtgcttca gtaagcaaaa gctatggccc agatggaaac
     3541 gaggcgtaca gtttaacttg ttcaggaatg gcccgtgatt ggggaatgtg tttaacaaag
     3601 gcaggagaac tgtgtggagc aaaaggatat aacatcctta gtataaatgg cgattccgga
     3661 gcattagtta cagccaaccc acaatcatta atggctggct cagtcatcaa tagaaacatg
     3721 ctcatatcct gcaagcagct ataggacata aaaagagcca accttgaggt acatttacca
     3781 taacatccgt tgcccgcacc acctcaggcg ccctcagggc gtttctgagg actccggcct
     3841 gaaacataca cctttctgtg cccatatcga gaaaaaacgc ccctgatgat ttctgcgtac
     3901 ctgcatggct atgcactcaa gtgaatgaag taagatttca gtctgcgatc accataaa
//
