LOCUS       NZ_CP091324             5651 bp    DNA     circular CON 09-AUG-2023
DEFINITION  Enterobacter sp. JH25 plasmid pDR25-5, complete sequence.
ACCESSION   NZ_CP091324
VERSION     NZ_CP091324.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25126227
            Assembly: GCF_021725435.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. JH25
  ORGANISM  Enterobacter sp. JH25
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Huang,J.
  TITLE     Characterisation of bla NDM-5 and mcr-9 co-occurrence in ST78
            carbapenem-resistant Enterobacter in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Huang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2022) Department of Clinical Laboratory, Jinhua
            Central Hospital, Mingyue street 351, Jinhua, Zhejiang 321000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/09/2023 03:30:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,103
            CDSs (total)                      :: 4,988
            Genes (coding)                    :: 4,891
            CDSs (with protein)               :: 4,891
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 36 of 97
            Pseudo Genes (incomplete)         :: 68 of 97
            Pseudo Genes (internal stop)      :: 13 of 97
            Pseudo Genes (multiple problems)  :: 19 of 97
            Pseudo Genes (short protein)      :: 1 of 97
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091324.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Enterobacter sp. JH25"
                     /mol_type="genomic DNA"
                     /strain="JH25"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:2923088"
                     /plasmid="pDR25-5"
                     /geo_loc_name="China:Jinhua"
                     /collection_date="2018-11-11"
     gene            complement(join(5594..5651,1..878))
                     /locus_tag="L2Y28_RS25450"
     CDS             complement(join(5594..5651,1..878))
                     /locus_tag="L2Y28_RS25450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_045626452.1"
                     /translation="MATYHLSVKFGGKGKALAHASYITREDKFSQRQDLEHTEHGNMPE
                     WARDEPAHFWQAADAFERANGSTYREIEIALPRELNEAQRLALVRDFVKQETGDKHAWT
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERSPEQYFRRYNAKFPARGGARKDSGHNL
                     TPTEQKQALKALRERWERTHNAHMKRYGFEQGFIDSRSLKTQGIDRKPEQHLGFEAAGR
                     LDDSQRQDIKDSRTFSVVDVPEQAKHIDHSDRSWMNASGIEVNRKELVRSLTHDISQQY
                     GREMAARVTKQGNVEIERVKSLSRGFSISR"
                     /name="MobA/MobL family protein"
     gene            1045..1431
                     /locus_tag="L2Y28_RS25455"
     CDS             1045..1431
                     /locus_tag="L2Y28_RS25455"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045282373.1"
                     /translation="MRISNVEWLKKRIAFIRKLGEKTARQRQIIDLLDDEASLSETERR
                     LLHVLATAEKNALQERDAAQKKAVQARISGQKNRRERNHRLFLAAGLMIDAGLVDTKTG
                     ELNFDHEKLLTALKRVRYDLETSA"
                     /name="hypothetical protein"
     gene            complement(2243..3022)
                     /locus_tag="L2Y28_RS25460"
     CDS             complement(2243..3022)
                     /locus_tag="L2Y28_RS25460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="WP_109018074.1"
                     /translation="MELPNIIQQFIGNSVLEPNKIGQSPSDVYSFNRNNETFFLKRSST
                     LYTETTYSVSREAKMMSWLSDKLKVPELIMTFQDEQFEFMITKAINAKPISALFLTEQE
                     LLSIYKETLNQLNAVAIFDCPFISSIDHRLKESKFFIDNQLLDEIDQDDFEAELWGDHK
                     TYLSLWNELNETRVEERLVFSHGDITDSNIFIDKSGEIYFLDLGRAGLADEFVDISFVE
                     RCLREDVSEETAKIFLKHLKNDMPDKRNYFLKLDELN"
                     /name="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
     gene            complement(3268..>3567)
                     /locus_tag="L2Y28_RS25465"
                     /pseudo=""
     CDS             complement(3268..>3567)
                     /locus_tag="L2Y28_RS25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004821790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /name="enoyl-CoA hydratase/isomerase family protein"
     gene            3625..4322
                     /locus_tag="L2Y28_RS25470"
     CDS             join(3625..3874,3874..4322)
                     /locus_tag="L2Y28_RS25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_095033700.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            complement(5061..5591)
                     /locus_tag="L2Y28_RS25475"
     CDS             complement(5061..5591)
                     /locus_tag="L2Y28_RS25475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012634455.1"
                     /translation="MASTEEKLLTLLLSAEHLKEAAKEQQKAVADSLAVARETLKGYRE
                     ISDSVAQQVRDEVRQEVQKMDVAGLIGERISADFQRLEKSVGNMKINADALDKEISIIR
                     SNIIAEYNTLRGYSWKWFAGVAVAFVVLIFATSWFVKTELQDNYNASAAVYQKVADLEK
                     LLTTEQKPEKKKN"
                     /name="hypothetical protein"
     ARG             complement(2247..3022)
                     /name="aph(3')-VIb"
                     /gene="aph(3')-VIb"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3')-VIb"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.07"
                     /coverage="100.0"
     Typing          complement(4..878)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.863"
                     /coverage="94.0"
                     /evalue="0.0"
ORIGIN
        1 ccctgttttg tgacccgtgc agccatctcc cgcccgtact gctgcgagat atcatgcgtc
       61 agagatctga ccagttcctt ccggtttacc tcgataccgc tggcattcat ccagctacgg
      121 tcgctgtggt cgatatgttt cgcctgttca ggcacatcca cgactgaaaa cgtccggctg
      181 tctttaatat cctgcctctg gctgtcgtcc agtcgtccgg ctgcttcaaa accgagatgc
      241 tgttccggtt tccggtcgat accctgtgtt ttcagtgacc ggctatcaat aaaaccctgc
      301 tcaaagccat atcgtttcat atgcgcgttg tgcgtacgct cccagcgctc ccgcagggct
      361 ttcagtgcct gtttttgttc tgtaggggtt aaattatgcc ctgagtcctt gcgcgcgccg
      421 ccacgcgccg gaaatttggc gttatagcgc ctgaaatact gttcgggact gcgctcaata
      481 ccgtcattga cgcgctggct catcatgata tgggcgtgcg gctgttcgcc gccgtcaatg
      541 ctggctttcg gattgtggat cgcaaacgtc catgcatgtt tgtcgcccgt ttcctgcttc
      601 acaaaatccc ggacaagcgc cagccgctgc gcttcattca gctcgcgggg tagagcgatc
      661 tcaatttccc tgtacgttga accgttggca cgttcgaacg catccgcagc ctgccagaaa
      721 tgggcgggtt catctctcgc ccattccggc atattgccgt gttcggtgtg ctcgagatcc
      781 tggcgctggc tgaacttatc ttcccgcgta atgtaactgg catgagcaag ggctttaccc
      841 ttgccgccaa atttcacgct gagatgatac gttgccatgt tgtgtcagcc cccggcaggg
      901 cgcactgttt ctgcccgcag ggagaaacat ataagtgcgc tttactctaa tttaaagaat
      961 tatgagtaaa gataagtcat gcgacttctg accgcaagtt atatactttc cccgtcggaa
     1021 atatcgggat ggggaagtta aacgatgcgg atcagtaacg tcgaatggct taaaaagcgc
     1081 atagccttca tcagaaagct gggagaaaaa acggcacggc aacggcagat tatcgatctg
     1141 ctggatgatg aagcttcgct cagtgagact gaaagacgac tgctccacgt tctggcaacc
     1201 gctgaaaaaa acgcgttgca ggaacgggat gccgcacaga aaaaggcggt tcaggcgcgg
     1261 atttcagggc agaaaaaccg cagggaacgc aatcacagac tgtttctggc ggctggtctg
     1321 atgattgacg cgggactggt tgatacaaaa acgggtgagt tgaattttga ccacgaaaaa
     1381 ttactgactg cgctgaaacg ggtacgctac gatctggaaa catcggcgtg aacgtgcatt
     1441 ctgcaaggcc ggagaaatgc cgcttcctcg ctcactgact cgcgtcgctc ggtcgttcgg
     1501 ctgcggcgag cggtagggat tttcggatag gagcatgaat tttcttgctc cagatgtaca
     1561 aaagccccgt gacagaatca cggggcttga gtactctaat tcttgcagat acctgccagg
     1621 atcagacgtc acaatcaaat cctagcaggg ctggctagga atgtcactac cagcttaata
     1681 ccattcaacg acattaaacc catatctgca ccttgactct caggtctacg ccccggactc
     1741 cggcaggata aacaggcaag cggtgggaac ctgaccagct cgggaaagaa gctggctcta
     1801 cccggtctca cgttaaccaa taacgtacct gataaagtga gagtgaaggc agcggttgcg
     1861 gtgaagcgtg attgccaagg tctggcgcaa cacccacaaa atcgaagcag catacgaacg
     1921 gcctgatgct ggtcgcttcg ctccccgttt tctggtgtac ccaccacggg ggcattaggc
     1981 tttctttgcc ctaatatggg tttgcgactc tcaaatccgg gttgtggtgt aacacatatc
     2041 gccttcaccg aaatgggtaa tgataccaac gtgttgattt cgtattttca ctgacaccag
     2101 gatcatcctg atgttacaag gattgaatga caacagatta aaatagtcat caacaggttg
     2161 acagcattac cttttttcac ttatttattt aaaaattagc aacttaaaaa tacagctaag
     2221 tatttttaga tattgcttgg agttaattca attcatcaag ttttaaaaaa taattccttt
     2281 tgtcaggcat atcgtttttt aaatgcttta aaaatatttt agcagtttcc tcagatacat
     2341 cctctcttag gcaacgttca acaaaagata tatctacaaa ttcatctgct aatccagcac
     2401 gaccaagatc taaaaagtaa atttcaccag atttatctat aaaaatatta ctatccgtga
     2461 tatcgccatg agaaaaaacc aatctttctt caacacgagt ctcatttaac tcattccaaa
     2521 gacttaggta agttttatgg tctccccata attcagcctc aaaatcatct tgatctatct
     2581 cgtcaaggag ttggttatca ataaaaaatt ttgactcttt taaccgatga tcaatgcttg
     2641 aaataaatgg gcaatcaaaa atagcaactg catttaactg attgagtgtt tccttataga
     2701 tagaaagcaa ttcttgctct gttaaaaaaa gcgctgaaat tggttttgca ttgatcgctt
     2761 tagtgatcat gaattcaaac tgctcatcct gaaaagtcat gatgagttca ggcaccttta
     2821 atttatcaga gagccaactc atcattttcg cttcacgaga gacactgtat gtggtctctg
     2881 tatataaagt gctagatcgc ttaagaaaaa aagtttcatt atttcgatta aaagaataaa
     2941 catccgatgg cgactgacca attttatttg gctctaaaac gctgtttcca ataaattgtt
     3001 gaataatatt aggcaattcc atcaagtttt ccttttattc agcattaaaa acctcgcaaa
     3061 tgcgaggcct agtaaataga tgatcttaat taggttcact gtagcaaaaa tatggcttat
     3121 tgtctataga gggtcaatgc atcaaacttt tgtcattacc tctcaattgc ataaatagcg
     3181 ctaggcttaa ggaaaatggg ttgctgtatt tctctgtctc aaatctcaag aataagctag
     3241 agcagaatat tagacagcag caagacctca ggatgcagac aaatcgcgga gtagttgctc
     3301 caaatcctgt tcgcccactt ttgtttgagc acctgcctgc aagccgccac gaatgagttt
     3361 ttcagcagcc ggacgcgcaa acagaccagc ccatgccttg tgctctcgaa ggaagtcttc
     3421 acttaaatcc gttggtggga ttgctttctt tgcagcggcg atgacccctt caggtaaagc
     3481 tgcaatattt gtggcgatgc gatgaacaaa agcgtctaac tcatttgccg gaagcgcacg
     3541 atttacccaa ccatatcgtt cggctagcag gtgatgctgc caacttactg atttagtgta
     3601 tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca
     3661 gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg ctatctctgc
     3721 tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca acccggtacg
     3781 caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac cgcccgcatt
     3841 atgggcgttg gcctcaacac gattttccgc catttaaaaa actcaggccg cagtcggtaa
     3901 cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac gaacagtggg
     3961 gatacgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac aggctccgga
     4021 agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg cgtcttatga
     4081 gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg ctgtatgaat
     4141 cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga attgagcggc
     4201 ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg tcgttctcaa
     4261 aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa cactatcagt
     4321 aagttggagt cattaccacc gaaatgatgt gaagacttca tcattttagc aatcacaggt
     4381 tgccctaatc acttaacgct attattgttc tattgaagcc ttaacctaaa cccactcaca
     4441 aaacgtggat tatctggtta gtgattcgca gcaacaaagc gtcccggttc aaaacgaatc
     4501 gtaagacgta gtttcccttg atgtagggct tgccgatgga acaggcggct tttttattct
     4561 ccggcgtggg gggggatgct gttgacagaa atggaaataa gtaacatccg gaatattacg
     4621 cttattgccc ggcgtcttgc ctggattaat gccaaaagaa ctcatatcga tataccatcg
     4681 tttgcagaac tgcttttctc cggccaaaaa gcgctgttct tcgaacaata taattatttt
     4741 cactcacgac ggtacacaac ggcaggcacg tcaaagccac cggatttacc ataacatccg
     4801 ttgcgtgcac cgcctcagag gctctcaggg cggttttaag tcctgagcca taaaaacccc
     4861 cggcttctca ccgaaaaccg ctcagaatcg cataccgtga ttttcagccc catgcataac
     4921 catgcacctg actgactggc gagaacttcc ggcctgaatt caggccggaa gtctcaaaag
     4981 acgccccaaa ctcggctgaa aacggtgaac gggtgcgcaa tttattgtgc gaacgtgagg
     5041 cgttggacaa gcgcagcgcg tcagttcttt ttcttttccg gtttctgttc cgttgtcagc
     5101 agcttttcaa ggtctgccac tttctgatag acggcagcag acgcgttgta attgtcctgt
     5161 agttccgttt tcacaaacca gcttgtggca aatatcagta cgacaaaggc aaccgcaacc
     5221 ccagcaaacc acttccagct gtaaccccgc agcgtgttgt actccgcgat gatgttactg
     5281 cggataatgg aaatttcctt gtccagcgcg tccgcattga ttttcatgtt cccgacggat
     5341 ttttccagac gctgaaaatc agcgctgata cgctcgccaa tcagccccgc aacatccatt
     5401 ttctgcactt cctgccggac ttcatccctt acctgctgtg ccacactgtc cgatatttcc
     5461 cggtagcctt tcagggtttc ccttgccacc gccagcgaat ccgccacggc tttctgctgc
     5521 tcttttgcgg cctctttcag atgctccgcc gacagcagga gtgtgagaag tttttcttcg
     5581 gtgctcgcca tagttacctg cttatggaaa atccgcgtga cagggatttc acgcgctcta
     5641 tttcaacatt t
//
