LOCUS       NZ_CP091323            12474 bp    DNA     circular CON 09-AUG-2023
DEFINITION  Enterobacter sp. JH25 plasmid pDR25-4, complete sequence.
ACCESSION   NZ_CP091323
VERSION     NZ_CP091323.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25126227
            Assembly: GCF_021725435.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. JH25
  ORGANISM  Enterobacter sp. JH25
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter.
REFERENCE   1  (bases 1 to 12474)
  AUTHORS   Huang,J.
  TITLE     Characterisation of bla NDM-5 and mcr-9 co-occurrence in ST78
            carbapenem-resistant Enterobacter in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12474)
  AUTHORS   Huang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2022) Department of Clinical Laboratory, Jinhua
            Central Hospital, Mingyue street 351, Jinhua, Zhejiang 321000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/09/2023 03:30:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,103
            CDSs (total)                      :: 4,988
            Genes (coding)                    :: 4,891
            CDSs (with protein)               :: 4,891
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 36 of 97
            Pseudo Genes (incomplete)         :: 68 of 97
            Pseudo Genes (internal stop)      :: 13 of 97
            Pseudo Genes (multiple problems)  :: 19 of 97
            Pseudo Genes (short protein)      :: 1 of 97
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091323.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12474
                     /organism="Enterobacter sp. JH25"
                     /mol_type="genomic DNA"
                     /strain="JH25"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:2923088"
                     /plasmid="pDR25-4"
                     /geo_loc_name="China:Jinhua"
                     /collection_date="2018-11-11"
     gene            complement(join(12363..12474,1..305))
                     /locus_tag="L2Y28_RS25390"
     CDS             complement(join(12363..12474,1..305))
                     /locus_tag="L2Y28_RS25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283459.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_040233155.1"
                     /translation="MSDQKNNFDLQKQTLESKWGKEALSMGWSAIPTSLFFLQGALNMT
                     PTTFNVLLHLIIHWWKPYDWPHPSQESIAVRMGVSSRTVQRSLQELENYGLLTKIRTSK
                     DNPKYKGRNIYDMSLLVKSLNILTPDLNSKLNEK"
                     /name="helix-turn-helix domain-containing protein"
     gene            653..1327
                     /locus_tag="L2Y28_RS25395"
     CDS             653..1327
                     /locus_tag="L2Y28_RS25395"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_001259273.1"
                     /translation="MRTIVYVDGFNFYHGLVKRSGYKWLDLYKLFEEQLLPIRARESQL
                     IQVKFFTAEIKAKFATRGELAHQAQQAYHRALLQIYGEKLQIIKGYYSERQECAMTFNE
                     GYPPSKTDRTTVWKLEEKQTDVRMALEMYRDAIRGEAEQLVLCTNDSDMIPPIEMIKAD
                     RPELKIGIVVPREQDTSRVISSLASLGDWSLPYVSYEALRLALLPDKVPTRRKPILKPN
                     HW"
                     /name="NYN domain-containing protein"
     gene            2326..2649
                     /locus_tag="L2Y28_RS25400"
     CDS             2326..2649
                     /locus_tag="L2Y28_RS25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_029593203.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2858..>3292
                     /locus_tag="L2Y28_RS25405"
                     /pseudo=""
     CDS             2858..>3292
                     /locus_tag="L2Y28_RS25405"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3327..3866
                     /locus_tag="L2Y28_RS25410"
     CDS             3327..3866
                     /locus_tag="L2Y28_RS25410"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_236919607.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKRG
                     NRRIRKKSYAKLTVFS"
                     /name="MbeB family mobilization protein"
     gene            complement(4000..5577)
                     /locus_tag="L2Y28_RS25415"
     CDS             complement(4000..5577)
                     /locus_tag="L2Y28_RS25415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_001323889.1"
                     /translation="MRQNLPVTGRNLELPKDANILSTTSPQSHITYVNPDFIKISGFTE
                     EELLGQPHNIVRHPDMPPAAFEHMWSTLKSGRSWMGLVKNRCKNGDHYWVSAYVTPIAK
                     NGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSIISSA
                     MAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERARNISNNPLSQSLYTGR
                     TDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQAETDQ
                     IATAVNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSITALEGEIRQATQ
                     VIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLAA
                     RTQQSTTDIQSMISALQERAQSAVTVMEQSSRQAHTSVAHAEEAATALDGIGQRVNEIT
                     DMNAQIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQSAKSVAQLTSALSELA
                     KQFWEKRG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            5887..6447
                     /locus_tag="L2Y28_RS25420"
     CDS             5887..6447
                     /locus_tag="L2Y28_RS25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001161490.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQL
                     EALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATAGEP
                     KAQLAREFNISRETLYQYLRTDD"
                     /name="recombinase family protein"
     gene            6451..9417
                     /locus_tag="L2Y28_RS25425"
     CDS             6451..9417
                     /locus_tag="L2Y28_RS25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="WP_001138014.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRGD
                     ANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRREHA
                     QELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALSVID
                     RACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKPNSRH
                     MLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYATLAAV
                     VLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIGQALLEA
                     KESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRYTPALLEV
                     LELRAAPAAQGVLAAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPEGLDRKFYE
                     ICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAINPNSDQYLE
                     ERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQLLPRI
                     KITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESSPGLTY
                     AKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGGRGESTG
                     HVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLRIEEHYTD
                     TAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRPLIGGTLNIKHVRAH
                     WDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVEL
                     RRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYL
                     ERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSRRLEDGKFRPLRMPGKP"
                     /name="Tn3-like element TnAs1 family transposase"
     gene            9472..9687
                     /locus_tag="L2Y28_RS25430"
     CDS             9472..9687
                     /locus_tag="L2Y28_RS25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029590204.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            10392..11084
                     /locus_tag="L2Y28_RS25435"
     CDS             10392..11084
                     /locus_tag="L2Y28_RS25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="WP_000550475.1"
                     /translation="MHLLYVDESGTPSDPTLNHFVLSGISVFEKQTHWIEQEMNAIAAR
                     FNADDPYERELHGSPMRGGRDGWKKFPFDDRKQAIKDCLTLLTQKHRDIRIFACVVPKG
                     SLGGKDVITESFYQISSRFDMYLSRLHHQGATSRGVAIFDKSSTEKSIQSLARTFKDTG
                     YGYGKLRNFSEVPLFLDSKASRLIQLADLVSYSIFRKFELNDDEFYKIIENSFDYNNGK
                     VHGLYVAH"
                     /name="DUF3800 domain-containing protein"
     gene            complement(11264..11572)
                     /locus_tag="L2Y28_RS25440"
     CDS             complement(11264..11572)
                     /locus_tag="L2Y28_RS25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012564913.1"
                     /translation="MTEKLKGTASVLHQTKTYEELVQKHSVEVANGLLANAINTALPNA
                     GITSNDVAGFSKVTIALRTGEVDLAKTAEEANADAAAVSANIIAGLKASQKSKDEIK"
                     /name="hypothetical protein"
     gene            complement(11678..11914)
                     /locus_tag="L2Y28_RS25445"
     CDS             complement(11678..11914)
                     /locus_tag="L2Y28_RS25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006535655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000019317.1"
                     /translation="MSHPVGKHISKEQEHELNYWLKKRGFKESKENRTSLVWLIESAKL
                     GLGMPSNEHLEHAELDKYFEDNKGLWIDEFETK"
                     /name="hypothetical protein"
     Typing          2040..2213
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.286"
                     /coverage="100.0"
                     /evalue="1.22e-69"
     Typing          complement(1566..2073)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.151"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          2640..3826
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.697"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 gtacgaattt ttgtaagcaa tccgtagttt tcaagctctt ggagtgagcg ttgtactgtt
       61 cttgatgaaa cacccattct aactgcaatg ctctcctgag aggggtgtgg ccaatcgtag
      121 ggtttccacc agtggataat tagatggagt aagacattga atgttgtggg tgtcatattg
      181 agtgcaccct gtaagaagaa aagggatgta ggaatagctg accagcccat gcttaatgct
      241 tcctttcccc atttcgattc aagtgtctgt ttttgaaggt caaaattatt tttttgatct
      301 gacatatacc aacctgctaa gctgaagtct tctatgaaga cttcagctta gcaggttggt
      361 aaattaattc atataattag tacgacacca gcgcgcttat aatatcatta tatttaacta
      421 tacttaatct acactcacct cttggtagcg cgattaagtt acgcagctcc cttgaggctc
      481 taaacctccc tttaggtggg cagcgattag gttacgcgtc ttcctttagg agcttgaaac
      541 cgccctcttg gttagtttta accctgagga actacaactt aaactgtttg ctaacgttag
      601 tttttgttgt taaaaccgcc cttagaggcg gtttttttat gcgcgaaata aaatgagaac
      661 aatagtttac gtagatgggt tcaattttta ccatggattg gtcaaacgtt cggggtataa
      721 gtggctggac ctttataagc tctttgaaga acaactactt ccgataagag caagagagtc
      781 acagttgatt caggtgaagt tctttactgc tgagatcaaa gctaaatttg ctactcgagg
      841 tgagttagct caccaggctc agcaagctta ccatcgagca ttactgcaga tttatggcga
      901 gaagctacaa ataatcaaag gttactactc agaacgtcag gaatgtgcga tgaccttcaa
      961 tgaggggtat cctccatcga aaacggatag aacaacggtc tggaagttag aagaaaagca
     1021 gactgacgtt agaatggctt tagaaatgta tcgcgatgcg attcgtggcg aggcagagca
     1081 gttggttctc tgtactaacg attcggatat gataccgcca atagaaatga taaaagccga
     1141 tcgacctgaa ttgaaaattg gaatcgtagt gccaagggag caagatacta gtcgagtgat
     1201 tagtagtctg gctagtttag gggattggag cctgccatat gtatcctacg aagctttgag
     1261 attagcgcta ttgccagata aggttcccac taggcggaaa ccaatactga agccaaatca
     1321 ctggtaaaca gccggatctt cttgtgatcc ttttactctc gtcgtaatct catgccctgc
     1381 aaacaaaaaa accaccgcta ccaacggtgg ttcgtttgcc ggaatcaaga gctaccaact
     1441 ctttcttccg aaggtaactg gctttcacgg agcgcaggta ccaaaactgt ccttccagtg
     1501 tagccgcagt caggccacca cttcaagaac tctgtagaac actctactac tccgctccgt
     1561 gaatcctctt actaatggct accgccactg gcgatttttc gtgtctgttc cgggttggac
     1621 tcaagatgat agttaccggg acaacgtggt agtcgggctg aacggggggt tcgtgcatac
     1681 agtccagctt ggagcgaact gccttcccgg aaccgagtgt caggcgtgga atgagaaacc
     1741 gcggccataa cagcggagtg acaccggtaa accgaaaggc aggaacagga gagcgcacga
     1801 gggagccacc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact
     1861 gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct
     1921 tcgccgcggc cttatcgttt ccctgttaag tttcttcctg gcatctacca ggaaatttcc
     1981 tccccgcccg taagccggta ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2041 gagcgaggaa gcggaatatc tcctgtatcg catattctgc tgacgcgcta gtgccacctt
     2101 ttttctcctt ccacatgaag cacttcactg atttccgcat ccgtgccaac atagtaagcc
     2161 agtatacact ccgctagcgc tgcgtgactg gttcagggct gcgccccgaa acccgccaaa
     2221 agcctgacgc gcctgcggct tgtccaaccc cgcgccgacc gggaaaggct ttcccgcccg
     2281 tcccggcgtt atcaggaggc cggtttgtca gagaaacgga acaagatgct caccatgtgg
     2341 gtgacggagg acgagcaccg gcggctgctc gagcgctgtg acggcaggca gctggcggcg
     2401 tggatgcgac agacctgcct ggacgaaaag cctgcgcgca ccggaaagct tccctcactc
     2461 tcgccggcgc tgctgcgcca gcttgccggc atgggcaata acctgaacca gatagcccgc
     2521 cgtgttaacg ccggcggcgg taccggccat gacagggtac agattgtggc ggcgctgatg
     2581 gccatcgatg ccgggcttga gcggctgcgg cacgccgttc ttgagaaagg aacggacgat
     2641 gatcgttaaa tttcatccgc gcggccgcgg cggtggcgcc gggccggtgg actatctgct
     2701 gggtaaagac cggcagcgtg aaggcgccac cgtcctgcag gggaagccgg aagaagtccg
     2761 ggagctgatt gacgcgtcgc cttacgtgaa gaaatacact tccggcgtcc tgtcgtttgc
     2821 ggaggccgat ctgcctcccg gccagcggga aaaactgatg gccagcttcg agcgggttct
     2881 gatgcccgga ctcgataaag atcagtacag cattctgtgg gtggagcacg aggacaaggg
     2941 gcgtctggag ctgaactttc tgatccccaa taccgagctg ctgaccggca ggcggctcca
     3001 gccgtactac gaccgcgccg accggccgcg tatcgatgcc tggcagacca tcgtgaacgg
     3061 cagactgggg ctgcacgacc cgaacgcgcc ggaaaaccgc cgggcgctgg tcacgccgtc
     3121 cggactgccg aaaacgaagc aggaggccgc agaagccatc acgcgcggac tgctgaccct
     3181 tgcttcgtcc ggggagctga aaacccgtca ggatgtcact gaggcgctgg aacggtcagg
     3241 ttttgaggtg gtgcgcacca cgaaaagcag tatcagcatt gccgacccgg acggggggcg
     3301 aaacatccga ctgaagggag ccatctatga gcagtctttt aacgctggcg aaggacttag
     3361 agcagcaatc gaaagcgcag aagcagagta ccggcgagat gctgaaagcc gcattcagcg
     3421 agcacgagca gtctgtcaga gcggaactga gcgcaagcgc gaggagaatc agcgacgcca
     3481 tcctcgccca cgagcagagt atgagcgagg cgatggagaa aaaccggcgg agcgtactgc
     3541 ggactgccgg gcggacatgg ctcaccatcc tgatggtgtc agcgctgctg atcggcacga
     3601 gcgggagcat tctgtggtgg caggggcagc agataaccga caattacacg catcttcgtc
     3661 agcaggaaga taccctggcg aaaatgaccg cccggacgtg gggagtgcgc tatcaggaga
     3721 gcagcgacgg ccggaggttt cttatcctgc cgccggggat gcaaaccgaa gccatccctt
     3781 acgacgggac gacatggatc cgcctgaaac gggggaaccg cagaattcgg aaaaaatcgt
     3841 acgctaagct aacggtgttc tcgtgacagc tctttgacta ggctttctaa ggccattctg
     3901 atagccctga cttcctgaaa agccatggct aaaatttgtg cggctaaaag ggataaccga
     3961 tggtaaagta agttatccct gtcgagatac tgaaaagcgt tatcctcgtt tttcccaaaa
     4021 ctgttttgcc agttcgctca gagcgctagt taactgagcg acagatttcg cactttgcaa
     4081 attattctgc ccggtctgta cattggtatc agcagcatcg cgtatattga taatactgcg
     4141 gtttatgtct tcacttactg ctccctgctg ctcgaccgca gtcgctattt gcgcgttcat
     4201 gtcggtaatt tcgttaacgc gttggccaat tccatcaaga gctgtagctg cttcctctgc
     4261 gtgagctaca ctcgtgtgcg cttgccgact actttgctcc atgactgtaa cagcggattg
     4321 cgctcgctct tgtagagcgc tgatcatgct ttgaatatcc gttgtcgatt gctgtgtgcg
     4381 agcagcaaga ctgcgaacct catcggcgac aacagcaaaa ccacgcccct gctcaccagc
     4441 acgcgcggcc tcaattgctg cgttgagtgc caacaaattc gtttgctcgg cgatccctcg
     4501 tataacgtca agaacttttg atatctcgtt actttgacct tcaagctcat gaataacctg
     4561 agtggcttgc ctaatttcac cttcaagggc agtgattgac tggcttgtgt gggctaccag
     4621 acgctggcca gatgccgtct cagtgtctgc tcttccggcc gcatctgcag catgctgtgc
     4681 attgctcgca acctcttgaa tgcttgccac catttggttt actgccgttg ctatttgatc
     4741 tgtctctgcc tgctgctcaa ctgtaagtac attgcttgac tcaatatcct ttagtaggcc
     4801 tcgggtgtgt tcgctaagcc gatttgatgc atcacctatg cgacctacta tggcgcctgt
     4861 ttcagcttgc atcattcgta aagcaaactc tatttggcca aactcatcgg tgcgcccagt
     4921 gtagagggat tgacttaatg ggttattgga aatattcctg gctctttcaa ccagtcttcc
     4981 aagaggagag agaatagcca aaacactaac agagcttaag cttcctgaca ttaaagtggc
     5041 taacaataag ctgcttattg atgtatcagt aagcatgccg gcagccattg cgcttgatat
     5101 aatactaccc catatgagca agagtatttt cacggaaaag ctagcagcca atttcggcct
     5161 cgcggccttc ccgcttctca attgagcata taatttttcc gcagccaaaa cctgctcagg
     5221 ttcaggcttg gtccttacag actggtattc aacaatcgaa ccattcttag ctattggcgt
     5281 tacataagca cttacccaat agtggtcgcc atttttacag cgatttttta ctagccccat
     5341 ccatgagcgg ccagatttta atgtactcca catatgctca aatgcagcag gcggcatatc
     5401 tgggtgtctt acgatgttgt gaggctggcc taatagttct tcctcagtga aaccactgat
     5461 tttaatgaag tcaggattaa cgtacgtgat atggctttga ggggaggtag tcgaaagaat
     5521 attggcatct tttgggagtt ctaagtttcg acccgtcact ggtaaattct ggcgcataag
     5581 aacctcaagg gttggctgtt ttattttatt gttttcggca ttaagcccaa tttcttgagc
     5641 gttacgataa agctagcatg gaaacgatag gtgcaagcaa gttaagggtt gcatcgcgca
     5701 tgtcaatcta ggctataccc taacttgatg tcaggcaggg ccgcgccgct tcgtcagaat
     5761 agagtctgct ttcccatttt ttgacacatg cccgcgaagg ttatagattt cagcctgaca
     5821 gaaatgggct ttgaggcaca acggaacaga aagtgcactt aagccgcctt caaccaagga
     5881 gacatcgtgc aggggcaccg catcggctac gtccgggtca gcagctttga ccagaacccg
     5941 gaacgccagc tggaacaaac ccaggtgagc aaggtgttca ccgacaaggc atcgggcaag
     6001 gacacccagc gcccccagct cgaagcgctg ctgagcttcg tccgcgaagg cgatacagtg
     6061 gtggtgcaca gcatggatcg gctggcccgc aacctcgatg acctgcgtcg cttggtacag
     6121 aagctgactc agcgcggcgt gcgcatcgag ttcctgaagg agggcctggt gttcactggc
     6181 gaggactcgc cgatggccaa cctgatgctg tcggtgatgg gggccttcgc tgagttcgag
     6241 cgcgccctga tccgcgagcg gcagcgtgag ggcatcgcct tggccaagca gcgtggcgcg
     6301 taccggggcc gcaagaaagc cctgtccgat gagcaggctg ctaccctgcg gcagcgagcg
     6361 acggccggcg agcccaaggc gcagcttgcc cgcgagttca acatcagccg ggaaaccctc
     6421 taccagtacc tccgcacgga cgactgacac atgccgcgtc gcttgatcct ctcggccacg
     6481 gagcgggaca ccctgcttgc gctgccggaa agccaggatg acctgatccg ctactacacc
     6541 ttcaacgact ccgacctgtc gctgatccgc cagcgacgcg gcgacgccaa ccgcctcggc
     6601 ttcgccgtgc agctctgcct gctgcgctac cccggttacg cgctgggaac cgacagcgag
     6661 ctgcccgagc cggtcatcct gtgggtggcg aagcaagtcc aggccgagcc ggcgagctgg
     6721 gcaaagtacg gcgagcgcga cgtgacccgt cgcgagcatg cccaggaact gcgcacctac
     6781 ctgcaactgg ccccgttcgg cctgtccgac ttccgcgccc tggtgcgcga gctaaccgag
     6841 ctggcccagc agaccgacaa aggcttgctg ctggccggtc aggccctgga gagcctacgg
     6901 cagaaacgac gcatcctgcc ggcgctgagc gtgattgacc gggcctgctc ggaagccatt
     6961 gcgcgagcca atcggcgggt ctaccgcgcc ctggtcgaac cactcacgga ctcgcatcgg
     7021 gccaagctgg acgagctgtt gaagctcaag gccggcagca gcatcacctg gttgacctgg
     7081 ctgcgccagg caccgctgaa acccaactct cggcacatgc tggaacacat cgagcggctg
     7141 aagacatttc agttggtgga cttgcccgaa ggcctgggcc ggcacatcca ccagaaccgc
     7201 ctgctcaagc tggcccgcga gggtgggcag atgacgccca aagacctcgg taagttcgag
     7261 ccgcagcgcc gctacgcgac cctggccgcc gtggtgctgg agagcaccgc gaccgtgatc
     7321 gatgagctgg tcgatctgca tgaccgcatc ctggtcaagc tgttcagcgg cgcgaagcac
     7381 aagcatcagc agcagttcca gaagcagggc aaggcgatca acgacaaggt gcgcctgtac
     7441 tccaggatcg gccaggcgct gctggaagcg aaggaaagcg gcagcgaccc ctatgccgcc
     7501 atcgaggcgg tgattccctg ggacgagttc accgagagcg tcagcgaggc cgagctgctg
     7561 gcccggccgg aaggcttcga ccacctgcac ctggtcggcg agaacttcgc caccctgcgc
     7621 cgttacacgc cggccttgct ggaggtgctg gaactgcgcg ccgcgccggc cgcgcaaggc
     7681 gtgctggcag ccgtgcagac cctgcgtgag atgaacgccg acaacctgcg caaggtgccg
     7741 gccgatgcac ccacggcctt catcaagccg cgctggaagc cgctggtgat caccccggaa
     7801 ggcctcgacc ggaaattcta cgaaatctgc gccctgtccg agctgaagaa cgccctgcgc
     7861 tccggcgaca tctgggtcaa gggctcgcgg cagttccgcg acttcgacga ctacctgctg
     7921 ccggccgaga agttcgccgc actcaagcgc gagcaggccc tgcccctggc gatcaacccg
     7981 aacagcgacc agtacctgga agagcgtttg cagctgctgg acgagcagtt ggccaccgtc
     8041 acccgcctgg ccaaggacaa cgagctgccc gatgccatcc tcaccgagtc agggctgaaa
     8101 atcaccccgc tggatgcggc ggtgccggat cgggcgcagg cgctgatcga ccaaaccagc
     8161 cagttactgc cgcgcatcaa gatcaccgaa ctgctgatgg acgtggacga ctggacgggc
     8221 ttcagccgcc acttcaccca cttgaaggac ggggccgagg ccaaagacag gacgttgctg
     8281 ctgtccgcaa tcctcggtga tgcgatcaac ctcgggctga ccaagatggc cgagtcgagc
     8341 cccggcctga cctacgccaa gctgtcctgg ctgcaagcct ggcacatccg cgacgaaacc
     8401 tattcggcgg ccttggccga gctggtcaac caccagtatc gccacgcctt tgccgcccac
     8461 tggggcgacg gcacgacctc atcctccgat ggccagcgct tccgcgcggg tggccggggc
     8521 gagagcaccg ggcacgtcaa cccgaagtac ggtagcgagc cgggacggct gttctatacc
     8581 catatctccg accagtacgc gccgttcagc acccgcgtgg tgaatgtcgg cgtccgcgat
     8641 tccacctatg tgctcgacgg cctgctgtac cacgagtccg acctgcggat cgaggagcac
     8701 tacaccgaca cggccggctt caccgatcac gtctttgccc tgatgcacct gctaggcttc
     8761 cgcttcgcgc cgcgcatccg cgacctcggc gaaaccaagc tgtacgtgcc gcagggcgtg
     8821 caagcctacc cgacgttgcg cccgctgatc ggcggcaccc tgaacatcaa gcacgtgcgt
     8881 gcccactggg acgacatcct gcgcctggcc agctcgatca agcagggcac cgtcaccgcc
     8941 tcgctgatgc tgcgcaagct cggcagctac ccgcgccaga acggactggc cgtggccctg
     9001 cgcgagctgg gccggatcga gcgcacgctg ttcatcctgg actggctgca aagtgttgaa
     9061 ctgcgccgcc gcgtgcatgc cggcctgaac aaaggtgagg cgcgcaactc gctggccagg
     9121 gcggtgttct tcaaccgcct tggggaaatc agggatcgga gcttcgagca gcagcgctac
     9181 cgggccagcg gcctcaacct ggtgacggcg gctatcgtgc tgtggaacac ggtgtacctg
     9241 gaacgcgcca cccaggggtt ggtcgaggcc ggcaagccgg tggacggcga gctgctgcaa
     9301 ttcctgtcgc cgctgggctg ggagcacatc aacctaaccg gcgattacgt ctggcggcag
     9361 agccgcagac tggaagacgg gaagtttcgg cccttacgga tgcccggaaa accttagcgt
     9421 acgatttttt ccgaattctg cgggctcccc ctgaaacagg agtagcggaa aatgacagag
     9481 ctggaaaagc agttgctgag cgcattagag cagctacagc aggactactc gcaaaggctg
     9541 gacgaatggg agagcgcctt cgcggaatgg cggagcatgt gtggtcttat gcaacgggag
     9601 aacgcgatgc tgaacgagcg cgtgtcggac ttgagcacgc aggtgctgag tttaagcgag
     9661 cagctgcgcc gcttgtccgg gaactgaatg ccatcgagct ccggcagagg gagaatgagt
     9721 atcagaaaac actggagctg gagcgccagc cgcagaaaac ctaccgcggg ccgacacttt
     9781 aaaccatcca gacagctgac aacccgcacg aagaggggct cttatttctc ccgctaaata
     9841 ggttcgtgat caaaaaggtc acgacttatt caggagcggg aaatgaagct gttaaatctt
     9901 attgaatcat tgcttaactg cctatacctg gcggcggagc tgtgggactg gctggagaag
     9961 catttgtcgg tttttaaggc gttcgcgctg attttagtgt gactttacgc gccatgcatc
    10021 tggctgcatg actatgcatc agtgtaatat ccagcggctg cgtttctggt gttttttcgt
    10081 catgtctgtc gccactttct cgcgtctggc gccggaaacg cagccaaccc cgtttcagcg
    10141 gtgcgggcaa cggatgttat ggtaaataag agtgacgatg aagcaactat ccgattgaat
    10201 ttatgttatt taattgctca tgattatgat atgtgtataa attatttggg taaatatgcg
    10261 tttgcctgtt atcattaaaa ctgagtcagt tcgctgccca tagcggtgta tgcgattttt
    10321 atcgcactgg cttgcctaat tcatgaaaag gccgcgcaaa tgcgcggcct ttttcgttta
    10381 aggaatcttc gatgcatctc ttatacgttg atgagtctgg tacaccatca gaccctacac
    10441 ttaatcattt tgtcctttct ggtatctcag tgtttgaaaa gcagactcat tggatagagc
    10501 aagagatgaa tgctatcgca gccagattta atgctgatga tccgtatgaa agagaattgc
    10561 atggttcgcc tatgagaggt gggcgagatg gctggaaaaa atttcctttc gatgacagaa
    10621 aacaagcgat caaagattgc ctaacactcc tgactcaaaa gcatcgggat atccgtatat
    10681 ttgcgtgtgt tgtacccaag ggatccttag gtggaaagga cgtaataaca gaatcttttt
    10741 atcagatatc gtctcgtttc gacatgtatt tgagccgctt acatcaccaa ggagcaacat
    10801 ctcgaggggt ggccattttt gataaatcgt caacagagaa aagtattcag tctcttgctc
    10861 gtacgtttaa agatactgga tatggatatg gaaaacttcg taacttctct gaagtgcctt
    10921 tgtttttgga ttccaaagcg tcaaggctga ttcagcttgc ggatcttgta tcgtactcaa
    10981 ttttcaggaa gtttgagctc aatgatgatg agttctacaa gattattgag aattcctttg
    11041 actacaataa tggtaaagtt cacggacttt acgtagctca ctaattgaat cgctgtggag
    11101 agtttgtctc cgagtcagat gccgttgtgg ccaccatttt ccatcgatgc agagcctacg
    11161 ttgcatttct cattagcatc gcacaacgga tgttatagtg agtacagccc gttatggata
    11221 taaaaaaagc caccattcag gtggcttttt accccgctga taatcacttt atttcatcct
    11281 tgctcttttg tgatgctttc aatcccgcaa taatatttgc tgaaacagct gccgcatcag
    11341 cgttcgcttc ttccgctgtt ttggctagat caacttctcc tgtgcgcaag gctatcgtta
    11401 ccttactgaa tccggcaacg tcatttgaag ttattcctgc gttaggcaaa gcagtattta
    11461 tcgcatttgc cagcaagccg ttagcgactt ctactgaatg cttctgaacc aattcctcat
    11521 aagttttggt ttgatgcaga acgctagccg ttccttttag tttctcggtc atgtaaacct
    11581 cttgtctgtg ataatttaga tgtgggattt tacatattgt tctgatttta aatggataaa
    11641 tcccacgtta tggaatttat ctcagatgta acagagatta ttttgtctca aattcgtcaa
    11701 tccataaccc tttgttatct tcaaagtatt tatctaattc ggcatgctcg agatgctcat
    11761 tgctaggcat tcctaggcct agcttcgctg attcaatcag ccacactaat gatgttctgt
    11821 tttctttact ctctttgaac cccctctttt ttagccagta attaagttcg tgttcttgct
    11881 ctttcgatat gtgctttcct actggatgtg acatttttca cccttacgat tgggaaaact
    11941 tacgctgaca catggagcat tttagggatc cattcgttcc atctttgaac agtgtcattg
    12001 tcttacaaaa tggacattgc ttacctcttc cgccccggcc ttcgtttggt ggtgttctca
    12061 ctacagcatt gcaagaagta cagctatttc cgaagagggt cagcttgtta cagaaggggc
    12121 acttttttcc aggcatttaa ggctttccat taaacattaa tcttatttaa caaatatatt
    12181 attaagatgt tttccttgta aagcactatc ttagggtaca tttcattgtg attaaatttt
    12241 gggcgatggt gagtgtggag tgtaccggca atcccctgtt catcttgtcg gagtccgcga
    12301 tacagacctg atacatggta aatctgctac gacggtggcc tgattttaaa tgtattttgc
    12361 ggttattttt cgttcagttt ggagttaagg tcaggagtaa gaatatttag tgacttaacc
    12421 aaaagagaca tatcgtaaat gtttcttcct ttgtacttag ggttgtcctt actt
//
