LOCUS       NZ_CP091226            20845 bp    DNA     circular CON 21-MAY-2024
DEFINITION  Enterococcus faecium strain 237 plasmid p237-b, complete sequence.
ACCESSION   NZ_CP091226
VERSION     NZ_CP091226.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23424739
            Assembly: GCF_023375445.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20845)
  AUTHORS   Biggel,M.
  TITLE     Florfenicol-resistant Enterococci from healty human
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20845)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 112x, 180x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore MinIon
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023375445.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 04:13:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,621
            CDSs (total)                      :: 2,530
            Genes (coding)                    :: 2,460
            CDSs (with protein)               :: 2,460
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091226.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="237"
                     /isolation_source="fecal sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p237-b"
                     /collection_date="2021"
                     /collected_by="ILS, University of Zurich"
     gene            complement(join(20660..20845,1..27))
                     /locus_tag="LQ068_RS12925"
                     /old_locus_tag="LQ068_12925"
     CDS             complement(join(20660..20845,1..27))
                     /locus_tag="LQ068_RS12925"
                     /old_locus_tag="LQ068_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002324171.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIEY
                     RTEYLELTNQTDFLLIRKQQEQEKN"
                     /name="hypothetical protein"
     gene            complement(48..899)
                     /locus_tag="LQ068_RS12930"
                     /old_locus_tag="LQ068_12930"
     CDS             complement(48..899)
                     /locus_tag="LQ068_RS12930"
                     /old_locus_tag="LQ068_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002287227.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILDL
                     TVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAGKL
                     FKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILFFVG
                     YVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKGIRSI
                     LKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDLELPMK
                     DK"
                     /name="hypothetical protein"
     gene            1082..2041
                     /locus_tag="LQ068_RS12935"
                     /old_locus_tag="LQ068_12935"
     CDS             1082..2041
                     /locus_tag="LQ068_RS12935"
                     /old_locus_tag="LQ068_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="WP_000222573.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHCLNRSRQTIHKVYLFF
                     KQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIRCS
                     ARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFKSITF
                     DCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDESF
                     IQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
                     /name="IS30 family transposase"
     gene            complement(2065..2361)
                     /locus_tag="LQ068_RS12940"
                     /old_locus_tag="LQ068_12940"
     CDS             complement(2065..2361)
                     /locus_tag="LQ068_RS12940"
                     /old_locus_tag="LQ068_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication control protein PrgN"
                     /protein_id="WP_000053907.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTRE
                     KTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
                     /name="replication control protein PrgN"
     gene            complement(2496..3989)
                     /locus_tag="LQ068_RS12945"
                     /old_locus_tag="LQ068_12945"
     CDS             complement(2496..3989)
                     /locus_tag="LQ068_RS12945"
                     /old_locus_tag="LQ068_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_000947691.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSKQ
                     LMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINTFV
                     VDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVIDVA
                     KKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSMKLES
                     DQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTLSLAYY
                     SSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELCQQWVSP
                     TLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLNLSKKELA
                     SQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLSKEKRLDYS
                     TRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
                     /name="primase C-terminal domain-containing protein"
     gene            4601..5554
                     /locus_tag="LQ068_RS12950"
                     /old_locus_tag="LQ068_12950"
     CDS             4601..5554
                     /locus_tag="LQ068_RS12950"
                     /old_locus_tag="LQ068_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000429439.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLFE
                     KDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGSPG
                     SEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAADYII
                     AMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQKTID
                     KYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYYEQHGD
                     IEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
                     /name="ParA family protein"
     gene            5526..5801
                     /gene="prgO"
                     /locus_tag="LQ068_RS12955"
                     /old_locus_tag="LQ068_12955"
     CDS             5526..5801
                     /gene="prgO"
                     /locus_tag="LQ068_RS12955"
                     /old_locus_tag="LQ068_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="WP_001196543.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVDP
                     PVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
                     /name="prgO"
     gene            5994..6248
                     /locus_tag="LQ068_RS12960"
                     /old_locus_tag="LQ068_12960"
     CDS             5994..6248
                     /locus_tag="LQ068_RS12960"
                     /old_locus_tag="LQ068_12960"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000199136.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYLP
                     DQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
                     /name="transposase"
     gene            6654..7816
                     /locus_tag="LQ068_RS12965"
                     /old_locus_tag="LQ068_12965"
     CDS             join(6654..6930,6930..7816)
                     /locus_tag="LQ068_RS12965"
                     /old_locus_tag="LQ068_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_086953888.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKWV
                     KQFKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANKHL
                     YPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSKRGI
                     QISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIVTDLT
                     YVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGK
                     EFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLEL
                     FDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
                     /name="IS3 family transposase"
     gene            7992..8489
                     /locus_tag="LQ068_RS12970"
                     /old_locus_tag="LQ068_12970"
     CDS             7992..8489
                     /locus_tag="LQ068_RS12970"
                     /old_locus_tag="LQ068_12970"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_228780308.1"
                     /translation="MCEKWAADITYIPTKKNGWCYLSSIMDLHTKRIISYTFSKRMTVD
                     CVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGI
                     ESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEK
                     MA"
                     /name="IS3 family transposase"
     gene            complement(8962..9534)
                     /locus_tag="LQ068_RS12975"
                     /old_locus_tag="LQ068_12975"
     CDS             complement(8962..9534)
                     /locus_tag="LQ068_RS12975"
                     /old_locus_tag="LQ068_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000170424.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRDR
                     PKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTSMG
                     RMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELLTSG
                     KSYKEVEAITGFSRSTLFRIKKKIEES"
                     /name="recombinase family protein"
     gene            complement(9550..10155)
                     /locus_tag="LQ068_RS12980"
                     /old_locus_tag="LQ068_12980"
     CDS             complement(9550..10155)
                     /locus_tag="LQ068_RS12980"
                     /old_locus_tag="LQ068_12980"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="WP_000599739.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTFK
                     GLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEIIKK
                     YVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDLEIR
                     YLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
                     /name="protein adenylyltransferase Fic"
     gene            complement(10353..11033)
                     /locus_tag="LQ068_RS12985"
                     /old_locus_tag="LQ068_12985"
     CDS             complement(10353..11033)
                     /locus_tag="LQ068_RS12985"
                     /old_locus_tag="LQ068_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            <11109..11759
                     /locus_tag="LQ068_RS12990"
                     /old_locus_tag="LQ068_12990"
                     /pseudo=""
     CDS             <11109..11759
                     /locus_tag="LQ068_RS12990"
                     /old_locus_tag="LQ068_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /name="C40 family peptidase"
     gene            11756..12688
                     /locus_tag="LQ068_RS12995"
                     /old_locus_tag="LQ068_12995"
     CDS             11756..12688
                     /locus_tag="LQ068_RS12995"
                     /old_locus_tag="LQ068_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="WP_001224319.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVLL
                     GLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSIDN
                     RMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQLITE
                     GENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSITTNEI
                     NEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTVSLTVE
                     YIDQQTKATQVSQFDLVLEKNGSNWKIVK"
                     /name="conjugal transfer protein"
     gene            12933..13049
                     /locus_tag="LQ068_RS13000"
                     /old_locus_tag="LQ068_13000"
     CDS             12933..13049
                     /locus_tag="LQ068_RS13000"
                     /old_locus_tag="LQ068_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="WP_001791010.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
                     /name="tetracycline resistance determinant leader peptide"
     gene            13065..14984
                     /gene="tet(M)"
                     /locus_tag="LQ068_RS13005"
                     /old_locus_tag="LQ068_13005"
     ARG             13066..14981
                     /name="tet(M)"
                     /gene="tet(M)"
                     /gene_name="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            15178..16554
                     /gene="tet(L)"
                     /locus_tag="LQ068_RS13010"
                     /old_locus_tag="LQ068_13010"
     ARG             15179..16551
                     /name="tet(L)"
                     /gene="tet(L)"
                     /gene_name="tetracycline efflux MFS transporter Tet(L)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            17118..18380
                     /gene="mobV"
                     /locus_tag="LQ068_RS13015"
                     /old_locus_tag="LQ068_13015"
     CDS             17118..18380
                     /gene="mobV"
                     /locus_tag="LQ068_RS13015"
                     /old_locus_tag="LQ068_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000119405.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYD
                     LKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFE
                     ESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKF
                     PEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVK
                     SDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDND
                     RLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSK
                     ISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNE
                     FELTHNREVKKERSRDQGMSL"
                     /name="mobV"
     gene            18625..19524
                     /locus_tag="LQ068_RS13020"
                     /old_locus_tag="LQ068_13020"
     CDS             18625..19524
                     /locus_tag="LQ068_RS13020"
                     /old_locus_tag="LQ068_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_025192414.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHIL
                     EFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKVVA
                     EVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMRATE
                     VTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKVQMIR
                     PKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYGGLLKE
                     IHKKLNLDGFCCKVLNKE"
                     /name="protein rep"
     gene            complement(19547..20227)
                     /locus_tag="LQ068_RS13025"
                     /old_locus_tag="LQ068_13025"
     CDS             complement(19547..20227)
                     /locus_tag="LQ068_RS13025"
                     /old_locus_tag="LQ068_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_002328852.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDYRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     ARG             15269..16554
                     /name="tet(45)"
                     /gene="tet(45)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="76.35"
                     /coverage="93.45"
     Typing          complement(2497..3989)
                     /name="rep_cluster_185"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          18605..19492
                     /name="rep_cluster_1018"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="88.0"
                     /evalue="0.0"
     Typing          17119..18377
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.19"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagcacaatc ctgtatagag gaatcatttt caaacgattc tccataatta tttatctttc
       61 attggaagtt ctaaatcact actgttccaa aaaatgtatg gtttgttttg acgaatatta
      121 cttccatcta accacaattt tccgatgcca tatcgtaggc ttgcttcgac ttcataaaaa
      181 ttgtagatct cttttcttcg atctattgct ttcatttcct ggccattttt taaaatagat
      241 ctgatgccct taacagtaga ttcatggata ctcgtgtgaa cgatatccgt attgatcaat
      301 tcgtcatatc ttccaaaatc aacattgtac tcataaccaa gaagtgtcgg aatcaggtca
      361 atttcaattt ctttttcaat tgaacgttta ataagtttcg tggatacata cccgacaaaa
      421 aagagaataa tacccacttc aaacaaatac atgtgtccat ttacaaagtg aataaacgtc
      481 aataaaaaat ccttcactcc ttttgttgtt cttccagatt gttcaatttg ttttattaaa
      541 gtcgctagaa ttgcatttac aacaattaaa tcgaataggc ttttgaaaag ttttcctgct
      601 gttaattttt gcctggtaaa ttgcatgact aattgagtaa aaatagacaa tagcatgaca
      661 aaaatcaacg tcattagcac tgtaaaaatg tctgatgctg caatcctatt gggatcattt
      721 atcaagtaat aagccgttcc aaatactcca atcgagtaaa cggttaaatc aagaataagt
      781 cgtgccccgt aactacaaag ttttcctaat ggccataatt gcaataccca aaatttcttt
      841 cgtttgctat ttcttttcag aggaagccag gtgtgttcaa tccagtcgtc cgatttcatt
      901 tcgatttctt ccctaaattc tttcctttaa cgttttcctt tgctacttta gcagcgatag
      961 gttgctcaat gaaccaattt tttaagtacg ccgattgtaa aattaagcta gacaactaaa
     1021 aaagtcattt gtgctacact caaaatagtt ccgtccaaag aattataagg agtgagtaca
     1081 aatgacctat acccatctta caacggatga acttgtaatg atagagtctt atttcaaaat
     1141 aaatcaatct gttgcgaaaa ctgcgcattg cttgaatcgt tcaagacaaa cgatccataa
     1201 agtatacctg ttcttcaagc aaggaaaatc agccttagag tactatcaac agtataagaa
     1261 aaacaaatca aactgtggta gacgcccgct tgttttacct gaggaacaat cagaatatat
     1321 tcaaagaaag gttgttcaag gatggacacc cgatgtgatt gttggtcgtg cagcgtttcc
     1381 tattcgttgt tctgctcgta ccatttatcg tatgttcaaa aaggggctat ttaatccttc
     1441 tgacttaccg atgaaaggca agcgtaaacc aaatggacat caagaaagac gtggaaaaca
     1501 agctttccgt cgctctattc atgaacgtga aaaagattat agccaattct caaatgagtt
     1561 tggtcacctt gaaggtgaca ctatcgtagg tttgaaacat aaaagtgctg taattacctt
     1621 agttgagcga ttatcaaaag ttatcatcac attgaaaccg tgtggtagac aagcgattga
     1681 tattgaaaac aaattaaatc attggtttga atctgtaccg aaaaatctat tcaaatccat
     1741 cacttttgat tgtggaaagg aattttcaaa ttggaaacag atcagtaatg ccaatgatat
     1801 tgccatttat ttcgctgatc caggaacgcc gtctcaaaga ggcctaaacg agaattctaa
     1861 cggattgtta cgtagagatg gtttattgaa atctatggat ttcaattcag tagatgaatc
     1921 ttttattcaa tctgtcgcat cgaaacgaaa taatattcct agaaaatcac tgaattatcg
     1981 aacacctttg gaagtatttt tgagttacgt aagtattgat gatttgtcta acttaatttg
     2041 acaattaaga gtaaataaaa actactactg gatatacttc ttcctttttc ttaactcgtt
     2101 agctatttta tactcaatat actcctgctc taacacacac aaacaatcat atacctgatc
     2161 catagtctcc ccagaagcta aggacaaccc atgaagaaac tcaatcggca aagaagcaac
     2221 tgttttttcc ctggtaaccc aagaagacaa agtcccctgc ttaaaaccat aaagctcaca
     2281 gaacacttcc acagataatc ccaaacgacc aatgataaac acattaacag gatgtgaata
     2341 gacataattt tttaacgaca tacaacttcc tccatgatac ataagactgc atacgcagtt
     2401 ataagtatac cacctacacg tagctaggca aagggagcaa cacctacaga agtatcatag
     2461 atatattgta taaggcaatt tgcacctttt atttttcaac ctgtatctac ttcaaaaaga
     2521 ttcacttctt taagagggtt caagggggtt gataattgtc taaatgtctc ttctactaag
     2581 ctctcgttga gtccaaatac ctctgtgatc cttgttgaat aatctagtct cttttcttta
     2641 cttagttgaa taaccctcgc gaacaacgtt ttgactgaag caatgatcat tccgccattt
     2701 cttcctcgtg ttaccttgta gaagacttta ttctcctctc gtaactcatt taagatctta
     2761 tcaagtgttc gcttgggcat cgttagttgt gaagccaatt ctttttttga caggttcaaa
     2821 taaggctcac tagcaagact tttttcgctt agataagcca atagatcttc tttccattca
     2881 tttttatgac tatattttct ttgatctcgt ggctttttaa atttccacca gccacgtcgt
     2941 tgaatgaaca gatcattatt ttttaaggtt ggtgaaaccc attgctggca caattcaata
     3001 atgaattctc tgcttgctgc ttgataatta ccagaataag cactacgtac aatctttact
     3061 aattctgctt cagatagcgg ttcctgtaac cgttcgttga atgtgaacat attgtattcg
     3121 caagtatcca atgcataacc tgaagagtaa taagctaaag aaagcgtgaa aatcacatta
     3181 tttcgaccaa gacgtccttc tccgccgacg atgtttgctt tatgaagtaa catatcgaac
     3241 cacggttcat ctacttgacg aatttctttt ttctcaggaa agacaattag ttttcgttct
     3301 cgatcttctt ttgttttttg atctgattca agcttcatcg accattgaat ccagtcagca
     3361 aaagaatact gatagttcat ttctttaaaa acaatattat tttcttgtgg gaatcgagca
     3421 attccgaagt ggttacatcc tagatccact ccggacaatt tttcagccaa ctgtagacga
     3481 attgtttttg aaattttttt tgctacatcg atcaccttga aattgctttt ctttgtgaca
     3541 tagcctggtg tctttagtac aaaataaact tgataaccat gatctgattc taaaattagc
     3601 gttggcatgt agcccacttg atctaaacag gctaaaataa tttcgccttg atggttctct
     3661 tttgaatcga tatcaactac aaatgtgttg atttgactta agtttttctc agaatgacct
     3721 ttaaccactg ttcgataatc atccgcataa gtaccaaagc gataggtatt aggtgtccaa
     3781 tgcgttagtt tcttttcgtt ctctaaaata gcttcttccg aagtaatgac cataccacgc
     3841 ccaacattca ttaattgctt gctacggtac gcaaagatag caccactcct actggaatca
     3901 atatgacctg caggtttcat ggtgctgttt cgatatttgt atgtacgtaa gccgttttta
     3961 agaatagctg aataaatttc gctcaacatc gattgtaacg tcctttctca aacaaaaaga
     4021 gaacacagtt tagctttaat ctcagccata gtatgtgttc tctcttcagt gtttaagaat
     4081 atttaattta agtacactaa ataaacgttc ttctgttgag ttgctcgcct ttttttggta
     4141 gaataaaggc aatcggaaaa ggtatccgat caacttttaa tataagtagt tggtagctac
     4201 ttacatattc ttgagacgag cgtgaacaca tgtcctaacc atgtgattca gaaataattg
     4261 attctgctaa aatcaattat ttccgtctct tttttttatg taatttttct gtttaaagag
     4321 taccactgct tttgctactt tgtaaatagt gaattatctg tggtttctat agaattaagt
     4381 ttacctcttt tcactctaaa ttgtcaacgc aaaacgcaca aatatatttt gacattttga
     4441 atatatctga tatactgaat atagcaaata tatttagtat atccactata ttccttataa
     4501 agtctgaatt cttatcaatt tggagtatat tagatatatc gaatatatta agtatatgaa
     4561 gtatactaaa tatatttagt ataggaaatt ggagggaact atggaaaact tacggactgc
     4621 atttaaaaca tcagataaac taggaaaagt tgtaatgctt acaggtagaa aaggcggcat
     4681 tggtaagact acagacaatg atttattagc gattgtttct agtcaacttt ttgaaaaaga
     4741 cgtgttgcta attgattatg accaacaaag aaatactact tccaacattg gatcaacgta
     4801 tcaaattact agctttgatc gatccatgtc tgcagcaatc aaaaaagggg actgggtttc
     4861 aggtattact caagtaagtc cacatttata tattatggct ggctctcctg gatctgaaga
     4921 acttaatgaa tacttgtccg aaaaatatcc tgatcgtcgt aagagatcat tggccttcat
     4981 taaaccttta gaagaattga gaaaaaattt cgattatatc tttatcgact gcccaccatc
     5041 aacagataat gtggttcgtg cattcttaac tgctgcagat tatattattg caatgcaaga
     5101 attaaaacgg tacgcaatgg aaggaacaga agattttatt aataaagttt tagttccgat
     5161 tgtcacgaat tttgaagagt ctcatcttca aatcattgga attttgcctg ttcttttctc
     5221 agtgcgacgt tcctctcaac acgcaaacta tcaaaaaaca attgataagt atggtgaatc
     5281 aaacattttt aaaagcattg ttaaaggctc ggatcggtta gaaatgtatg gtgagaatgg
     5341 tatccaatta aatgattatg tagatcgaag atggtgggct atttttgcag acatctttac
     5401 agaattagaa gaaagaattg aatactatga acaacatggt gatattgaag gatttgatta
     5461 tacacctaaa tatgctgact caatggccaa tcatatccta cctcttggaa aggagattaa
     5521 actaaatggc attattacca acgaacaaag ataagaagaa aattggtgct gaagatattt
     5581 caattaaaga tgatattaat gtcgagttag gggaacgaaa gtacactgcg aaagaaagaa
     5641 aaccagttca agtagatcct cccgtactaa aaaccattcg ggatcttagt tatgcaaaag
     5701 atattcctat gtatgaaata gtcaaacttg caatggacgc ctatgttgat actttaaacg
     5761 acgaggaacg gattatatat aatagacgat caaaagatta aaatatagta agtatactag
     5821 atatagtaaa tatatttagt atacttacta tatttttagc tttattaatc ctaatccttc
     5881 aaaatatgtg tattgtttgg gggtatacaa catttggtgt tagtatcaaa tcttagacag
     5941 cttttcgtag agacaagaat aaattaaact tatctacgag aggatgaatt tttatgaccc
     6001 gatatgaaga aaattttaaa caaatgattg ttgaactgaa tcaaactgga cgttctgttc
     6061 gagggttagc gaaagaatat ggcttatctg aagcaacgat ttacaaatgg aagaatttat
     6121 atttacctga tcagtccaca ggactgactg gaaaagaagt agctgaactg agaaaagaaa
     6181 atgctcgttt aaatgaggaa cttgaaatct taaaaaaagc cgcagccata ttctctcgga
     6241 aaacctaaat tcgcttgttc aatttattga aaaatggtgt aaggattaca ctgtttcttt
     6301 gttgtgtcgg ttattagaaa tccctagaag tgtctattat ttttataaga ataaaccgtt
     6361 gacagctaca gaaatcagaa ataataagtt gaaaaagaag atttcaacaa ttttttttac
     6421 gaataaacaa cgctatggtg ccacaaagat ccatcaagtt ttattaaaag aagggatttc
     6481 agtatctctt aaacatgtcc aaaagctaat gaaacaatta aatttaaggt cgattgtagt
     6541 taaaaaatat agacctcaaa ggtctaataa accgatcatg gataggctac aattaactag
     6601 acagaaaaat taaggtgtgt agactagaag aaaacatacc aggaggaatt tttatgtcta
     6661 agagaacacg aagaactttt tcacaagaat tcaagcaaca aatcgtcaat ctttacttag
     6721 ctggaaagcc acgtgtagaa atcattcgag aatatgaact aacggcttca gcatttgaca
     6781 aatgggtaaa gcaatttaaa acgagtggtt cattcaaaga aaaagataat cttacgcctg
     6841 aacaaaaaga attgttagaa ctacgtaaaa gaaaccagca attagaaatg gaaaatgata
     6901 ttttaaagca agcagcgctg atattcggac gaagagacaa gtaatcgatg cgaataagca
     6961 tctttaccct atatcagcga tgtgcagaat attaggtcta tcacgtcagt cctattatta
     7021 tcaatcaaaa ccaaagaaag acgaatcaga acttgaagaa gtagtcgctg aagaatttat
     7081 ccgcagccga aaggcctacg gctcaagaaa aataaaaaaa gccttatcaa aacgaggcat
     7141 tcagatcagc cgacgaaaaa ttagtagaat catgaaaaat agaggattaa aatcgagcta
     7201 tactgttgct tattttaaag tacatcattc tacttgcaat gaagccaaaa cgacaaacgt
     7261 attgaatcgt aaattcttaa gagacaaccc attagaagcg atcgtaacag acttgactta
     7321 tgtacgagtc gggaaaaaat ggaattatgt ctgtttcatt ttggatctgt tcaatcgaga
     7381 aattctcggc tattcttgtg gagaacataa agatgccgtt ctagtaaaaa aagcatttag
     7441 ccgtatcaaa caacctctga cagaggttga gatttttcat actgatcgtg gaaaagagtt
     7501 tgataaccaa gctattgatg aattattaac aacttttgac atcaatcgat cattgagtca
     7561 taaaggctgt ccttttgata atgccgtagc tgaatcaact tataagtcgt tgaaagtaga
     7621 atttgtctat caatacacat ttgaaacctt acaacaattg gatttggagt tatttgacta
     7681 tgtcaattgg tggaaccacc ttcggttgca cggtacactt ggctacgaga caccggttgg
     7741 ttaccgtaac cagagattgg cgcagcgaat ccttgataat gagctcggat gtgctaacgc
     7801 tagcgaggca gtctaacttt aactgttagc tcctgccgaa gatcgtcaca tccgaggagg
     7861 ctcattgtca aggacaatcg gaatagcata cggaaagaag ttctgcacct tataaaattt
     7921 gtcaaaaaaa ctgttgccat tccatcattt caaaagagaa tctcttaaat caggactttt
     7981 ctactgaaac gatctgtgag aaatgggcag ctgacattac gtacattcct actaagaaaa
     8041 atggttggtg ttacttatct tcaatcatgg atttacacac gaaaagaatt attagttata
     8101 cattttcaaa acgaatgact gtggattgtg tcattcaaac attgaataaa gcaaaaatac
     8161 attatcacat tccagaagga atgattctac acactgactt gggcagccaa tacacagcaa
     8221 gagaagtgga acaatggctt aaaaccaaca aaataaggca ttcttatagt agaaaaggaa
     8281 caccttatga taatgctgga atcgaatctt tccatgcctc attgaaaaaa gaagaagtct
     8341 acacgactag ctactcagat tttgaagaag caaatcgagc gctatttagc tacattgaag
     8401 gattttataa ccgaaatcga atccatagct cgattcacta tctaacccca caggaatttg
     8461 aagaattagc aaaagaaaaa atggcttaac cgtctctact aaaatgtgtc caagatattg
     8521 actcaaatcc agtaatatca caataggaat gctgtttatt atattagcag cagtgctgat
     8581 ttttatgata gttatttggt tcttaaatga aagatctaaa gacaatcaac accgtaggga
     8641 tatggagata aaatcaatag agtcaataga ttatttatcg ataataacac taaaaaagcc
     8701 acgttcctaa acgtgactta cgctgaacga tatgtgttgt ttggtagacg aacaaatcgt
     8761 tcattataaa tatatgatgg tatcctaata tagtaagcgt ggctagcata cctttaatta
     8821 ctttaccttt tacagttacc atacacattt tttgagcaga aatcaagtaa ataaaaaaat
     8881 attatatttc acaacatgct aatagactca tatctagaac gcattaagta tttacctgtc
     8941 tggttcgctg agacttcttt tttaactttc ttcaattttc ttctttattc taaaaagcgt
     9001 actgcgactg aaaccggtta tagcttcaac ttccttatac gatttacctg aagtaagcag
     9061 ttcatatgcg tgcctcttct tcggtgtgat tgttgctttc ggacgtcctt ctctgaaatt
     9121 aggattattc tttcttgcaa acatttttcc ttcttgggta cgttccacaa tcatatcacg
     9181 ttccatttct gctacactca acaacgtccg tgtcaccatt cgccccatgg acgtattttc
     9241 aatcgttcct agattcaaaa ccttgatcgt tacgttatca tcgagtaaag gttcaatgat
     9301 ctctaatgct tctctcgtgt tacgagcaaa tcgatcaagc ttcgtaacaa ctatttcatc
     9361 tccaggcttc agaccagcca acagctgatt gaattttggc cgatcccgtt ttgtacccgt
     9421 aaatttttct gaaaaaatat tcttcttctc tacgccgttc tcaagcaact gttgaatttg
     9481 ttcttttgta ctttgagaag cggttgagac acgtgcatat ccatatttcg tcatttggtc
     9541 cacctacttt tacaaatcat ctatcgtata ctcagaatac ccttcataga aatagctgac
     9601 atcgattcct ttcatatata actcgcgatc gtcaatttta tcagtcagag cattggaaat
     9661 tagatagcgt atttccaaat cgtttatggg acttcgctcc attgcagaaa gatagtctgc
     9721 tttatcgatt aagttccaat ctacaacttt ttgcaattct tcctttaaaa tgagatccag
     9781 ccaaattcta gtacttcggc catttccttc tcgaaatggg tgagcgatat tcatctccac
     9841 atatttcttg ataatctctt caaaagaagt ttgtggcatc tgatcaatat gctgtaacga
     9901 atgttctaaa tacataactg gtgcaaatcg aaaatttcct tttgcaatat tgaccgatcg
     9961 gatttttccg gcaaaatcgt aaatatccga aaatagaaat tcatgaattt cagataaacc
    10021 tttaaatgta ccaacttcga tttcatttat ttttccacaa tcgtacaatt ctttcgcttt
    10081 ttgtttactg agtttttctt caaccttagc caactcaact tgattggtta atcccaattt
    10141 attctctaaa atcatgcgaa atctccttaa ctttatgaca taagtttatg aaacactctc
    10201 aaccccatta tatcgcgatt ttgtttatgt gtcaaaaact attaagtttc tgacccccga
    10261 tagatttttc aagtgtctct attttgcaca gtctagttgg ttctgttgca aagttttaaa
    10321 taaagaataa aatcccttac ggtatctatg atttaagctg ggattcccaa taataccttg
    10381 atttcagtac agaccgaaaa cccgaagaga gtgccttctt ttcgggtttt cttatataat
    10441 cctcgaatgg cttccatgcc tttaatcgtg gtagaggcag tgcgtaaact tcgatagaat
    10501 ttattgcgtc tctttactgg acgatggtct tgttcaatca aattattcag gtatttaatg
    10561 gtacgatgtt ctgtcccttg ataaaagccg tattctttta gtttcttaaa ggcacttgta
    10621 atagaggggg ctttatctgt gactacaacc ttcggttcat caaactgctt cactaaccgc
    10681 ttaagaaaag cataggctgc ttgtgtgtcc cgttttttac gtaaccaaat atccaaggtt
    10741 aaaccatctg catcgatggc tcgatacaaa taatgccatt ttcctttaat tttgatgtac
    10801 gtttcatcca ttttccatga ataaaaggat tttttatttt tctttttcca aatttgatag
    10861 agtagtttgc catattcttg cacccaacga taaatcgtcg tatgagaaac gttaatgcca
    10921 cgatcatata agatttcttg aacttcacga tagctaaggt tataacgaag atagtagccc
    10981 acggctacaa taatcacatc ctgctgaaat tgctttcctt taaaatgatt catcgtcatt
    11041 cctcctgcta tctttttcta ttattctacc ttatttgata gtagatttaa aactttgcaa
    11101 cagaaccatt attagccagt agcgaaaggc tcagtgtaga tttagaatcg gttatccagt
    11161 cctacaatta tggtggtggt ttcttagggt atgtggctaa tcgtggaaat aaatatacct
    11221 ttgaactggc tcaaagtttc tcaaaagagt attcaggtgg cgaaaaagtg tcttacccca
    11281 atcccatagc catacctatc aatgggggct ggcgatacaa ctatggcaat atgttttatg
    11341 tgcaactggt aacgcagtat cttgtcacaa cagagtttga tgatgatacg gtacaagcca
    11401 tcatggacga agcactgaaa tatgagggct ggcgatacgt ttacggtgga gcttccccga
    11461 ctacttcttt tgattgtagc ggactgacac aatggacgta tggaaaagct ggaattaact
    11521 taccacgaac cgcacaacag caatatgatg tgacccagca tatcccacta tcggaagcac
    11581 aagctggcga tttggttttc tttcattcta cctataacgc tggctcttat attactcatg
    11641 ttgggatata ccttggcaat aaccgtatgt ttcatgcagg cgacccaatc ggttatgccg
    11701 acttaacaag cccctactgg caacagcatt tagtgggagc aggacgaatc aaacaatgag
    11761 aaaggaagat ttaatgatga aatttagaaa aaatcagaat aaagaaaaac agataccaaa
    11821 ggaaaagaaa cctcgtgtct ataaggtcaa tcctcataaa aaggttgtga ttgccttgtg
    11881 ggtactttta gggcttagtt tcagctttgc gatattcaag cactttacag ctatagatac
    11941 tcatactatt cacgaaacaa ctatcataga aaaggaatac gttgatactc atcatgtaga
    12001 aaattttgta gagaactttg cgaaagtcta ctattcatgg gagcaatccg ataagtccat
    12061 tgataatcga atggaaagtc taaaaggcta tctgacagat gaacttcaag ctctcaatgt
    12121 tgatacagta cgcaaagata ttcctgtatc gtcttctgta agaggatttc agatatggac
    12181 ggtagagcca actggcgaca atgagtttaa tgtaacctac agtgtagacc agctcattac
    12241 agagggagaa aatacaaaga ccgtccactc tgcttatata gtgagtgtct atgtagatgg
    12301 ttctggaaat atggtactgg ttaagaatcc gaccattacc aacataccta agaaatcaag
    12361 ttataaacca aaagccattg aaagtgaggg gacggttgat tccattacaa ccaatgaaat
    12421 caatgagttt ttaacgacgt tcttcaagct ctatcctaca gcgacagcca gtgaactttc
    12481 ctactatgtg aatgacggga tattaaaacc aatcggaaaa gagtacatct ttcaagaact
    12541 ggtaaatcct attcacaatc gtaaggataa tcaagtcacg gtatcgctga cagtggagta
    12601 tatcgaccag cagaccaaag caacgcaggt atctcaattt gatttggtac ttgaaaagaa
    12661 cgggagtaat tggaagattg taaaataaca aatattggta catgattaca gatactttgt
    12721 aatcatgtac tctttttgat aaaaaattgg agattccttt acaaatatgc tcttacgtgc
    12781 tattatttaa gtgactattt aaaaggagtt aataaatatg cggcaaggta ttcttaaata
    12841 aactgtcaat ttgatagcag gaacaaataa ttagatgtcc ttttttagga gggcttagtt
    12901 ttttgtaccc agtttaagaa tacctttatc atgtgattct aaagtatcca gagaatatct
    12961 gtatgctttg tatacctatg gttatgcata aaaatcccag tgataaaagt atttatcact
    13021 gggattttta tgcccttttg ggtttttgaa tggaggaaaa tcacatgaaa attattaata
    13081 ttggagtttt agctcatgtt gatgcaggaa aaactacctt aacagaaagc ttattatata
    13141 acagtggagc gattacagaa ttaggaagcg tggacaaagg tacaacgagg acggataata
    13201 cgcttttaga acgtcagaga ggaattacaa ttcagacagg aataacctct tttcagtggg
    13261 aaaatacgaa ggtgaacatc atagacacgc caggacatat ggatttctta gcagaagtat
    13321 atcgttcatt atcagtttta gatggggcaa ttctactgat ttctgcaaaa gatggcgtac
    13381 aagcacaaac tcgtatatta tttcatgcac ttaggaaaat ggggattccc acaatctttt
    13441 ttatcaataa gattgaccaa aatggaattg atttatcaac ggtttatcag gatattaaag
    13501 agaaactttc tatggaaatt ataatcaaac agaaagtaga gctgcaccct aatatgtgtg
    13561 tgatgagctg tacggaacct gagcaatggg atgtggtaat agaaggaaat gatgaccttt
    13621 tagagaaata tatgtccggt aaatcattag aagcattaga actcgaacaa gaggaaatca
    13681 gaagatttca gaattgctcc ttgtaccctg tttatcatgg aagcgcaaaa agcaacatag
    13741 ggattgagca gcttatagaa gtgataacga ataaatttta ttcatcaaca tacagaaaga
    13801 agtctgaact ttgcggaaat gtcttcaaaa ttgaatattc ggaagaaaga caacgtcttg
    13861 catatgtacg cctttatggc ggaatcctgc atttgcggga ttcggttaga atatcggaaa
    13921 aggaaaaaat aaaaattaca gaaatgtata cttcaataaa tggtgaatta tgtaaaattg
    13981 ataaggctta ttccggggaa attgttattt tgcaaaatga gtttttgaag ctaaatagtg
    14041 ttcttggaga tacaaagcta ttgccacaga gagagagaat tgaaaatccg ctccctctgc
    14101 tgcaaacaac tgttgaaccg agcaaacctc aacaaaggga aatgttactt gatgcacttt
    14161 tagaaatctc cgacagtgac ccgcttctac aatattatgt ggattctacg acacatgaaa
    14221 ttatactttc tttcttaggg aaagtacaaa tggaagtgat tagtgcactg ttgcaagaaa
    14281 agtatcatgt ggagatagaa ctaaaagagc ctacagtcat ttatatggag agaccgttaa
    14341 aaaatgcaga atataccatt cacatcgaag tgccgccaaa tcctttctgg gcttccattg
    14401 gtttatctgt atcaccgctt ccgttgggaa gtggaatgca gtatgagagc tcggtttctc
    14461 ttggatactt aaatcaatca tttcaaaatg cagttatgga agggatacgc tatggttgcg
    14521 aacaaggatt atatggttgg aatgtgacgg attgtaaaat ctgttttaag tatggcttat
    14581 actatagccc tgttagtacc ccagcagatt ttcggatgct tgctcctatt gtattggaac
    14641 aagtcttaaa aaaagctgga acagaattgt tagagccata tcttagtttt aaaatttatg
    14701 cgccacagga atatctttca cgagcataca acgatgctcc taaatattgt gcgaacatcg
    14761 tagacactca attgaaaaat aatgaggtca ttcttagtgg agaaatccct gctcggtgta
    14821 ttcaagaata tcgtagtgat ttaactttct ttacaaatgg acgtagtgtt tgtttaacag
    14881 agttaaaagg gtaccatgtt actaccggtg aacctgtttg ccagccccgt cgtccaaata
    14941 gtcggataga taaagtacga tatatgttca ataaaataac ttagtgtatt ttatgttgtt
    15001 atataaatat ggtttcttgt taaataagat gaaatatttt ttaataaaga tttgaattaa
    15061 agtgtaaagg aggaattaat tatgaagtga aatgaatgta acagggttca attaaaagag
    15121 ggaagcgtat cattaaccct ataaactacg tctgccctca ttattggagg gtgaaatgtg
    15181 aatacatcct attcacaatc gaatttacga cacaaccaaa ttttaatttg gctttgcatt
    15241 ttatcttttt ttagcgtatt aaatgaaatg gttttgaacg tctcattacc tgatattgca
    15301 aatgatttta ataaaccacc tgcgagtaca aactgggtga acacagcctt tatgttaacc
    15361 ttttccattg gaacagctgt atatggaaag ctatctgatc aattaggcat caaaaggtta
    15421 ctcctatttg gaattataat aaattgtttc gggtcggtaa ttgggtttgt tggccattct
    15481 ttcttttcct tacttattat ggctcgtttt attcaagggg ctggtgcagc tgcatttcca
    15541 gcactcgtaa tggttgtagt tgcgcgctat attccaaagg aaaatagggg taaagcattt
    15601 ggtcttattg gatcgatagt agccatggga gaaggagtcg gtccagcgat tggtggaatg
    15661 atagcccatt atattcattg gtcctatctt ctactcattc ctatgataac aattatcact
    15721 gttccgtttc ttatgaaatt attaaagaaa gaagtaagga taaaaggtca ttttgatatc
    15781 aaaggaatta tactaatgtc tgtaggcatt gtatttttta tgttgtttac aacatcatat
    15841 agcatttctt ttcttatcgt tagcgtgctg tcattcctga tatttgtaaa acatatcagg
    15901 aaagtaacag atccttttgt tgatcccgga ttagggaaaa atataccttt tatgattgga
    15961 gttctttgtg ggggaattat atttggaaca gtagcagggt ttgtctctat ggttccttat
    16021 atgatgaaag atgttcacca gctaagtact gccgaaatcg gaagtgtaat tattttccct
    16081 ggaacaatga gtgtcattat tttcggctac attggtggga tacttgttga tagaagaggt
    16141 cctttatacg tgttaaacat cggagttaca tttctttctg ttagcttttt aactgcttcc
    16201 tttcttttag aaacaacatc atggttcatg acaattataa tcgtatttgt tttaggtggg
    16261 ctttcgttca ccaaaacagt tatatcaaca attgtttcaa gtagcttgaa acagcaggaa
    16321 gctggtgctg gaatgagttt gcttaacttt accagctttt tatcagaggg aacaggtatt
    16381 gcaattgtag gtggtttatt atccataccc ttacttgatc aaaggttgtt acctatggaa
    16441 gttgatcagt caacttatct gtatagtaat ttgttattac ttttttcagg aatcattgtc
    16501 attagttggc tggttacctt gaatgtatat aaacattctc aaagggattt ctaaatcgtt
    16561 aagggatcaa ctttgggaga gagttcaaaa ttgatccttt ttttataaca ggaattcaaa
    16621 tctttttgtt ccattaaagg gcgcgattgc tgaataaaag atacgagaga cctctcttgt
    16681 atctttttta ttttgagtgg ttttgtccgt tacactagaa aaccgaaaga caataaaaat
    16741 tttattcttg ctgagtctgg ctttcggtaa gctagacaaa acggacaaaa taaaaattgg
    16801 caagggttta aaggtggaga ttttttgagt gatcttctca aaaaatacta cctgtccctt
    16861 gctgattttt aaacgagcac gagagcaaaa cccccctttg ctgaggtggc agagggcagg
    16921 tttttttgtt tcttttttct cgtaaaaaaa agaaaggtct taaaggtttt atggttttgg
    16981 tcggcactgc cgacagcctc gcagagcaca cactttatga atataaagta tagtgtgtta
    17041 tactttactt ggaagtggtt gccggaaaga gcgaaaatgc ctcacatttg tgccacctaa
    17101 aaaggagcga tttacatatg agttatgcag tttgtagaat gcaaaaagtg aaatcagctg
    17161 gactaaaagg catgcaattt cataatcaaa gagagcgaaa aagtagaacg aatgatgata
    17221 ttgaccatga gcgaacacgt gaaaattatg atttgaaaaa tgataaaaat attgattaca
    17281 acgaacgtgt caaagaaatt attgaatcac aaaaaacagg tacaagaaaa acgaggaaag
    17341 atgctgttct tgtaaatgag ttgctagtaa catctgaccg agattttttt gagcaactgg
    17401 atccaggaga acaaaaacga ttttttgagg aaagttataa attattttcc gaacgatatg
    17461 gcaagcaaaa tattgcttat gcaacagttc ataatgatga gcaaacccct cacatgcatt
    17521 taggtgttgt gcctatgcgt gatggaaaac tgcaaggaaa aaatgtgttt aatcgtcaag
    17581 aactgttatg gctacaagat aaattccccg agcatatgaa aaaacagggt tttgagttga
    17641 agcgtggtga acgtggctct gaccgtaaac atattgagac agctaaattt aaaaaacaaa
    17701 ctttggaaaa agagattgat tttctagaaa aaaatttagc agttaaaaaa gatgaatgga
    17761 ctgcttatag cgataaagtt aaatcagatt tagaagtacc agcgaaacga cacatgaaaa
    17821 gtgttgaagt gccaacgggt gaaaagtcca tgtttggttt gggaaaagaa ataatgaaaa
    17881 cagaaaagaa accaaccaaa aatgttgtta tatcggagcg tgattataaa aacttagtga
    17941 ctgctgcgag agataacgat aggttaaaac agcatgttag aaatctcatg agtactgata
    18001 tggcgagaga atataaaaaa ttaagtaaag aacatgggca agttaaagaa aaatatagtg
    18061 gtcttgtaga gcgatttaat gaaaatgtaa atgattataa tgagttgctt gaagaaaaca
    18121 agtctttaaa gtctaaaata agcgatttaa agcgtgatgt gagtttaatc tatgaaagca
    18181 ctaaggaatt ccttaaggaa cgtacagacg gcttaaaagc ctttaaaaac gtttttaagg
    18241 ggtttgtaga caaggtaaag gataaaacag cacaattcca agaaaaacac gatttagaac
    18301 ctaaaaagaa cgaatttgaa ctaactcata accgagaggt aaaaaaagaa cgaagtcgag
    18361 atcagggaat gagtttataa aataaaaaaa gcacctgaaa aggtgtcttt ttttgatggt
    18421 tttgaacttg ttctttctta tcttgataca tatagaaata acgtcatttt tattttagtt
    18481 gctgaaaggt gcgttgaagt gttggtatgt atgtgtttta aagtattgaa aacccttaaa
    18541 attggttgca cagaaaaacc ccatctgtta aagttataag tgaccaaaca aataactaaa
    18601 tagatggggg tttcttttaa tattatgtgt cctaatagta gcatttattc agatgaaaaa
    18661 tcaagggttt tagtggacaa gacaaaaagt ggaaaagtga gaccatggag agaaaagaaa
    18721 atcgctaatg ttgattactt tgaacttctg catattcttg aatttaaaaa ggctgaaaga
    18781 gtaaaagatt gtgctgaaat attagagtat aaacaaaatc gtgaaacagg cgaaagaaag
    18841 ttgtatcgag tgtggttttg taaatccagg ctttgtccaa tgtgcaactg gaggagagca
    18901 atgaaacatg gcattcagtc acaaaaggtt gttgctgaag ttattaaaca aaagccaaca
    18961 gttcgttggt tgtttctcac attaacagtt aaaaatgttt atgatggcga agaattaaat
    19021 aagagtttgt cagatatggc tcaaggattt cgccgaatga tgcaatataa aaaaattaat
    19081 aaaaatcttg ttggttttat gcgtgcaacg gaagtgacaa taaataataa agataattct
    19141 tataatcagc acatgcatgt attggtatgt gtggaaccaa cttattttaa gaatacagaa
    19201 aactacgtga atcaaaaaca atggattcaa ttttggaaaa aggcaatgaa attagactat
    19261 gatccaaatg taaaagttca aatgattcga ccgaaaaata aatataaatc ggatatacaa
    19321 tcggcaattg acgaaactgc aaaatatcct gtaaaggata cggattttat gaccgatgat
    19381 gaagaaaaga atttgaaacg tttgtctgat ttggaggaag gtttacaccg taaaaggtta
    19441 atctcctatg gtggtttgtt aaaagaaata cataaaaaat taaaccttga tgggttctgt
    19501 tgcaaagttt taaataaaga ataaaatccc ttacggtatc tatgatttaa gctgggattc
    19561 ccaataatac cttgatttca gtacagaccg aaaacccgaa gagagtgcct tcttttcggg
    19621 ttttcttata taatcctcga atggcttcca tgcctttaat cgtggtagag gcagtgcgta
    19681 aacttcgata gaatttattg cgtctcttta ctggacgata gtcttgttca atcaaattat
    19741 tcaggtattt aatggtacga tgttctgtcc cttgataaaa gccgtattct tttagtttct
    19801 taaaggcact tgtaatagag ggggctttat ctgtgactac aaccttcggt tcatcaaact
    19861 gcttcactaa ccgcttaaga aaagcatagg ctgcttgtgt gtcccgtttt ttacgtaacc
    19921 aaatatccaa ggttaaacca tctgcatcga tggctcgata caaataatgc cattttcctt
    19981 taattttgat gtacgtttca tccattttcc atgaataaaa ggatttttta tttttctttt
    20041 tccaaatttg atagagtagt ttgccatatt cttgcaccca acgataaatc gtcgtatgag
    20101 aaacgttaat gccacgatca tataagattt cttgaacttc acgatagcta aggttataac
    20161 gaagatagta gcccacggct acaataatca catcctgctg aaattgcttt cctttaaaat
    20221 gattcatcgt cattcctcct gccatctttt tctattattc taccttattt gatagtagat
    20281 ttaaaacttt gcaacagaac cgttcgtcac atcttcgatc aatttctctt acgattttct
    20341 catccacatt tcttattctt acttccatat taattcctcc aatttaaaaa ggatagcgaa
    20401 aaggagccta tcctttttgc tatcctacta ccctcctttt actatccttc actatccttt
    20461 cactatccta tttttaaata ttttgttccc acatttattt ttgagaaacg taataggcac
    20521 agcctattgg aacacttttt ttaaaaagtg ttcccacgtt tccttatgct ctttacaaat
    20581 aaaggctttt agagacgttc tggaatttta gattccttga attgttccca ttcatcaatc
    20641 aattggctat aagtagccat taattttttt cttgctcttg ttgctttcga atcaacaaaa
    20701 aatcagtttg attcgttaat tctaaatatt ctgttcggta ttcaatgttc atcgtacccg
    20761 ttttactaca tagtaactga ttatgacgtt ctttcacttt gaataactgc tccttcttta
    20821 aggtttttaa ctccatgcca actat
//
