LOCUS       NZ_CP091181             7196 bp    DNA     circular CON 31-JAN-2024
DEFINITION  Aeromonas caviae strain SCLZS52 plasmid p5_SCLZS52, complete
            sequence.
ACCESSION   NZ_CP091181
VERSION     NZ_CP091181.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25038853
            Assembly: GCF_021609985.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas caviae
  ORGANISM  Aeromonas caviae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1  (bases 1 to 7196)
  AUTHORS   Zhang,L. and Li,Y.
  TITLE     To characterize the genomic characterization of Aeromonas caviae
            SCLZS52
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7196)
  AUTHORS   Zhang,L. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2022) Department of pathogenic biology, Southwest
            Medical University, No.1 Section 1, Xiang Lin Road, Longmatan
            District, Luzhou, Sichuan 646000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/31/2024 05:33:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,727
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,418
            CDSs (with protein)               :: 4,418
            Genes (RNA)                       :: 161
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 123
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 40 of 148
            Pseudo Genes (incomplete)         :: 111 of 148
            Pseudo Genes (internal stop)      :: 18 of 148
            Pseudo Genes (multiple problems)  :: 19 of 148
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091181.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7196
                     /organism="Aeromonas caviae"
                     /mol_type="genomic DNA"
                     /strain="SCLZS52"
                     /isolation_source="wastewater"
                     /db_xref="taxon:648"
                     /plasmid="p5_SCLZS52"
                     /geo_loc_name="China:Luzhou"
                     /lat_lon="28.89 N 105.44 E"
                     /collection_date="2019-08"
     gene            complement(join(6399..7196,1..45))
                     /locus_tag="L1871_RS23430"
                     /old_locus_tag="L1871_23425"
     CDS             complement(join(6399..7196,1..45))
                     /locus_tag="L1871_RS23430"
                     /old_locus_tag="L1871_23425"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139728811.1"
                     /translation="MKKIIISFFLIAATFVSVHAVSFDCSKARSYSEKEICSTPELSKK
                     DDLLAALYKKAKAATQDQQAFSSIRNALWMSRERCENTLCLTAWYDSAFAIYEAIRSKG
                     IPTTIGTPTPKGDALTAPESHDSKSALVDNEAEPTAVPFDVSFEVGMDLNDGKPVVRVR
                     TNLPEGTIFIIFVVNPINWGGTGYIAQHRAEVSPTGFAEFAPLTDQGEALRPGKYLTTI
                     NTIGSGLQPENVRAVIGKYGENLTGNKISEINIGVGLEKWIDQKFVFNLHQDGSITVE"
                     /name="hypothetical protein"
     gene            1140..1562
                     /locus_tag="L1871_RS23435"
                     /old_locus_tag="L1871_23430"
     CDS             1140..1562
                     /locus_tag="L1871_RS23435"
                     /old_locus_tag="L1871_23430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236277413.1"
                     /translation="MAQQFNRLTPHTAQILALKGAGASIAEIQRWLRARRIRVDESTIR
                     RFWSRVHTQAPQSLPDFDAAAEVLLLKAETKIRRKRCFNQTRSRLDSRTAEILAMKNAG
                     LSAAKIQLELDAKGLTVDESTVWQFLKKQQGKYGLI"
                     /name="hypothetical protein"
     gene            1591..2445
                     /locus_tag="L1871_RS23440"
                     /old_locus_tag="L1871_23435"
     CDS             1591..2445
                     /locus_tag="L1871_RS23440"
                     /old_locus_tag="L1871_23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008091974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_236544954.1"
                     /translation="MQQVQGREIKSVGSVRDYEQGLTRVAEWQQAEHPRLAQGLRGMTQ
                     ELAIAYLEQRAEQVSQSTLNQERQALQVMMQLVTHKLAPDARLPVVQSEKQIVEQSRAY
                     TQEQIALVVGAQNERNGLATEIAHAAGLRASELLTLQRIEERAPSDRPAREEKFTGRAP
                     GERYTVHGKGGLIREVRLPSELAARLEARRLDTPVRIDDRGVFRDSRYDIAGGHAWSRS
                     FSAASDRVLGWSAGAHGMRHSYAQERYGELQVRQGLSPHDAKETLSQELGHFRAEITEC
                     YLR"
                     /name="site-specific integrase"
     gene            2442..2579
                     /locus_tag="L1871_RS23445"
                     /old_locus_tag="L1871_23440"
     CDS             2442..2579
                     /locus_tag="L1871_RS23445"
                     /old_locus_tag="L1871_23440"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_160839568.1"
                     /translation="MIKQYSYRFLWVIGALIARYGLHLVQADAALVFHPDDAAREVING
                     "
                     /name="hypothetical protein"
     gene            2572..3072
                     /locus_tag="L1871_RS23450"
                     /old_locus_tag="L1871_23445"
     CDS             2572..3072
                     /locus_tag="L1871_RS23450"
                     /old_locus_tag="L1871_23445"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_134997962.1"
                     /translation="MAELVGWLVPMLVLGGSVGLLYYSFSDNEPELKQLTYEKGQLTAR
                     VAALEAGVAERDRDLEKLREQLRENESTLRYTKGELEKSLKRSGNAQEAAKRMKEIQGL
                     QMAESKRLLAMALNPSSTDEQRRAALQQLHDHLHHPPAHKGRIKRVKTKKGKNPWAREK
                     AVA"
                     /name="hypothetical protein"
     gene            3178..3420
                     /locus_tag="L1871_RS23455"
                     /old_locus_tag="L1871_23450"
     CDS             3178..3420
                     /locus_tag="L1871_RS23455"
                     /old_locus_tag="L1871_23450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein RepB"
                     /protein_id="WP_134997961.1"
                     /translation="MEELQAKWTFDQGGFAKALIVPTPMGQGYHLHLVKFGKQSETIVL
                     ERQRGGWRVFKSIDAAANTANGIGFRRIEIDLSSR"
                     /name="plasmid replication protein RepB"
     gene            complement(3602..4594)
                     /locus_tag="L1871_RS23460"
                     /old_locus_tag="L1871_23455"
     CDS             complement(3602..4594)
                     /locus_tag="L1871_RS23460"
                     /old_locus_tag="L1871_23455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_134997960.1"
                     /translation="MVKSASYASFVERLPHRPYCTDDPAQGLLIRPQATALAYRHIQHN
                     PPPHVSTLIFDIDRPDGYHAWDDAGLPAPNWVCVNLKNGHAHYGYLLATPVARTSAARQ
                     KPLRYLAAIERVLTHRLGADVGYAGLITKNPLHDDWLTIWHHSEAFPLDYLAEFCPDAD
                     LAAYSRRSRNEASGLGRNVTLFDEVREWAYSAVRAYWRPNGYEAWADAVRAACDDVNGG
                     FALECGGPLPISEIKATAKSIARWVWQRFTPSEFRAIQAKRGAKGGKIGSGSNGGKVSK
                     GGGRPSKAVELLPEVIRLKAMGYSNRDIAQDLGIGSASVSRYLSKISPE"
                     /name="replication initiation protein"
     gene            complement(4792..5100)
                     /locus_tag="L1871_RS23465"
                     /old_locus_tag="L1871_23460"
     CDS             complement(4792..5100)
                     /locus_tag="L1871_RS23465"
                     /old_locus_tag="L1871_23460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="WP_134997959.1"
                     /translation="MQFTIYRNKGNARAYPYLLDVQSDIIGELATRMVIPLFPLVAFTG
                     RPAQRLNPVIIVEGDKYLVMTQDMAAVRLAQLGDEVTGVQESRQLIKDAIDFLFDGV"
                     /name="CcdB family protein"
     gene            complement(5100..5339)
                     /locus_tag="L1871_RS23470"
                     /old_locus_tag="L1871_23465"
     CDS             complement(5100..5339)
                     /locus_tag="L1871_RS23470"
                     /old_locus_tag="L1871_23465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="WP_134997958.1"
                     /translation="MVAAAMRSKRTVSVTLEPSLLERARAAGINLSATLATALQNEISG
                     SEAERWKRENSAAIQTLNRITEEHGLLSDDYRAF"
                     /name="type II toxin-antitoxin system CcdA family
                     antitoxin"
     gene            complement(5396..5665)
                     /locus_tag="L1871_RS23475"
                     /old_locus_tag="L1871_23470"
     CDS             complement(5396..5665)
                     /locus_tag="L1871_RS23475"
                     /old_locus_tag="L1871_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005323888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_134997957.1"
                     /translation="METITHPTLVQLVAAGAVRVVVAVGQPGGWTLLVRYGLAERALAA
                     QRSKQLRVFRKLDTLQAYLQDIGVVRFEIDAAGFEVAPRRLLGS"
                     /name="hypothetical protein"
     gene            complement(5840..6118)
                     /locus_tag="L1871_RS23480"
                     /old_locus_tag="L1871_23475"
     CDS             complement(5840..6118)
                     /locus_tag="L1871_RS23480"
                     /old_locus_tag="L1871_23475"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139728809.1"
                     /translation="MSLKRTIVRKLVDVTHDWNHEVHEAIEARIRQAYSEMIHKEAHES
                     QSAVETSGEMLEKMRTFYYSRMSMTASLLLAGTAVIISVLALLVSLF"
                     /name="hypothetical protein"
     gene            complement(6127..6315)
                     /locus_tag="L1871_RS23485"
                     /old_locus_tag="L1871_23480"
     CDS             complement(6127..6315)
                     /locus_tag="L1871_RS23485"
                     /old_locus_tag="L1871_23480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236277412.1"
                     /translation="MIQRFKIENETDADFFLKYLLAKDENRSVIEIIVRANNLISDETL
                     RMYFIGKGKQMLEALAE"
                     /name="hypothetical protein"
     Typing          3239..3418
                     /name="rep_cluster_2037"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.503"
                     /coverage="96.0"
                     /evalue="6.29e-58"
ORIGIN
        1 aaaagtagct gcaattaaaa agaatgaaat aattatcttc ttcattttat tcgtctcata
       61 cccaatgaga tttcatcata caggtgaggc gataaatgcc aaccgagatc agatcggata
      121 gcaaaaaatg ggcaccggtt taccgtgacg ccccctttgt ggtcagcaca tccagaaccg
      181 gatctagata gccaatttcg acactggtcg catcgttacc ggcgcgagac cccaccatga
      241 caccaaacgg tctacaacga caaaagtgcg ccgcctcgcc cgccaaccca ccgtgcataa
      301 tgcccattat gttcaattgg ccttttcacc agggaaaacg ggccgttcgc cccgttctgg
      361 cggcagaggg gacatttaga gtgctcggac agggctgccg aggcggtttg gcggctgaaa
      421 gtttgtgacg ggctggcggg gggaaatacc gaccccggag agccgatctg gtttcggcca
      481 gatcgggcgg tgaactggcg attttggata atctcctcgc tgtccaccga ggacgccgct
      541 aggcccagtt gacgccctgg cctgactgcg tctcccggcc ccaaaccaac gtgcgcgcaa
      601 catgcggaat ccttcacaca tatttgtgtg aacaatttga ttgttgttgc catcatattt
      661 tgattttgat gattcatatt ccgcatttga tgtactgttc gccccaataa ccggtttcgc
      721 atcgtttttc gccgcaaccc cggctgtgtc caagcctgta aacggcttga acacagccgg
      781 ggttgcggcc tgtagctcat ttgcgccgga aaaatcactc cggtgacaat cgagtcggat
      841 gtggctattc tgggggggaa aactagcagt acgttttttt caggtatcgc acaacctgga
      901 cgcctcgggc ccgaaatgct ctgaaaatcc tgatgaccta gctgctaatt ggtcggcctt
      961 aagtggcacg ttctgccccg aacccctcgg gttcacagcc cccatccggc tcaaaatgcg
     1021 atgcgtaacg tggtgttagt caaaccctct ctggcgacac agagcaattg gcaagtcggc
     1081 gcaagccgcg acgttccgaa cgaacgttgg ttgttcttac aactcgccga gatcccccga
     1141 tggcccagca attcaaccgc ctcacccccc ataccgctca gatcctggcc ctgaaaggag
     1201 ccggagccag catcgccgaa attcaacgtt ggctgcgtgc caggcggatc cgcgtggatg
     1261 agtccaccat ccggcgtttc tggtctcgcg tacacaccca agccccccag tccctgccgg
     1321 actttgacgc cgccgccgaa gtcctgctgc tcaaggccga gaccaagatc cgtcgcaagc
     1381 gttgcttcaa ccagacccgc agcagactgg actcccgtac agccgagatc ctggccatga
     1441 aaaacgcggg tctgagcgcg gccaaaatcc aactggagct ggacgccaag ggcctgacgg
     1501 tcgatgaatc taccgtctgg caattcctca aaaaacagca gggaaaatat ggcctcattt
     1561 agagccccat cagcccaagc ggcttgggta atgcagcagg tgcagggccg agaaatcaaa
     1621 agcgttggct ccgttagaga ctatgaacag ggcctaaccc gagtggccga gtggcaacag
     1681 gccgagcatc cgcgcctggc gcagggattg cgcggcatga cccaagagtt ggccatcgcg
     1741 tacctggagc agcgggccga gcaggtcagt caatcgacgc tcaatcagga gcggcaggcg
     1801 ctccaggtga tgatgcagct cgtgacgcac aaactcgcac cggacgcacg cctgcctgtg
     1861 gtgcaatcgg agaagcagat cgtcgagcaa tcgagggcct acacccagga acagatcgcc
     1921 ctggtagtgg gtgcccaaaa cgagcgaaac ggattggcca cggagatcgc ccatgccgca
     1981 gggctgcggg catcggagct gctgacactg caacggatcg aggaacgcgc ccccagtgat
     2041 cggcccgcca gggaggaaaa attcaccggc agagcgcctg gcgagcgcta caccgtccat
     2101 ggcaaaggcg gcctgatccg cgaggtacgg ctgcccagcg aactggcggc ccgcctcgaa
     2161 gcgcgccgcc tggacacgcc cgtgcggatc gacgatcgcg gcgttttccg cgacagtcgc
     2221 tacgacatcg cgggcggcca tgcctggtcg cggtcgttct ccgccgcctc cgaccgcgtg
     2281 ctcggctggt cagctggcgc ccacggcatg aggcacagct atgcccagga gcggtacggt
     2341 gaattgcagg tgcggcaggg gttgagcccg cacgatgcaa aagaaaccct gtcccaggag
     2401 ctcggacatt ttcgggcaga gatcacggag tgctatctac ggtgataaag caatattcct
     2461 atcggttctt gtgggtgatc ggtgccctga tcgccagata tggcctgcac ctggtccagg
     2521 ccgacgcggc attggtgttt catccagacg acgcggccag ggaggtcatc aatggctgag
     2581 ctcgttggct ggctcgttcc gatgctggta ctggggggat cagtgggact cctctactac
     2641 tccttctccg acaatgagcc agaactcaaa cagctcacgt acgaaaaagg gcaattgacg
     2701 gcaagggtgg cagccctgga ggctggggtc gcggaacgcg accgcgatct ggaaaagctg
     2761 cgggagcagc tccgggagaa cgagtccacc ctgcggtaca cgaaagggga gctggagaag
     2821 agtctgaaac gctccggcaa cgcgcaagag gcggccaaac ggatgaaaga gatccaaggg
     2881 ctacagatgg cggaatcgaa gcggttgctg gccatggcgc taaacccatc atctacggat
     2941 gaacagcggc gggcggccct tcagcagctc cacgatcact tgcaccatcc cccggctcac
     3001 aaagggcgga tcaagcgagt taagaccaaa aaaggaaaga acccttgggc ccgcgaaaag
     3061 gcagtcgcat agcgccgcga tcagcctggc tctcctacct gcgctgacgg ttgcctttcg
     3121 gaaacggcgc gggcatcatc aaaaaacaca tttgattaat cgaattggtt atttgatatg
     3181 gaagaactgc aagcgaagtg gacgttcgat cagggcgggt ttgccaaggc cctgatcgtg
     3241 cccaccccaa tgggtcaggg ctatcacctg cacctggtga agttcggcaa gcagagcgag
     3301 accattgtcc tcgaacgaca gcgcggcggc tggcgggtat tcaagagcat cgatgcggcg
     3361 gccaacaccg ccaacggcat cggctttcgg cgcatcgaga tagacctgtc cagtcgttaa
     3421 gggccaagac aaagaccagc tccgtggaca acgcccccgc ccacagcccg caagcggtct
     3481 gctggacggc ctccggttcg ggcgcttgcc cacctcgcca agatcctctt tctcaggaca
     3541 agccatatca gaaacaaacg cccttcgggc gctgttatct gataaggctt gttgttttgg
     3601 atcactctgg cgagattttg gagaggtagc ggctaacact ggccgaacca atccccaaat
     3661 cttgggcaat atcgcggttg ctatagccca tggcttttag cctgatcacc tccggtaata
     3721 gctctaccgc tttgcttggc ctaccgccgc ccttggatac cttgccgcca ttcgagccgc
     3781 tgccaatctt gccacccttg gccccgcgct ttgcttggat ggctcggaac tcggacggcg
     3841 tgaaccgctg ccatacccac ctagcaatgc tcttggccgt ggccttgatc tcagaaatgg
     3901 gcaacgggcc gccacattcc agcgcaaacc cgccgttaac gtcatcacag gcagcgcgga
     3961 cggcatccgc ccaagcctca tacccgttag gccgccagta tgctctaacg gcgctgtagg
     4021 cccattctcg cacctcatcg aacagggtaa cgttacgccc taacccgctg gcctcgttgc
     4081 gactacggcg gctgtaggcc gctaaatcgg catcgggaca aaactcggcg agataatcga
     4141 gggggaacgc ctccgaatgg tgccatatcg ttagccaatc gtcgtgtaac gggtttttgg
     4201 tgatcaaccc tgcataccct acgtccgcac ctagccgatg cgttaaaaca cgctctatgg
     4261 ccgctagata gcgcaacggc ttttgccgtg cggcactggt tcgtgccaca ggggtggcaa
     4321 gcagatagcc ataatgggca tgaccgttct tcaagttgac acaaacccaa ttcggcgcgg
     4381 ggagtccggc atcatcccat gcgtggtaac cgtccggcct gtctatgtca aaaatcaacg
     4441 tgcttacatg gggcggcggg ttgtgttgga tatggcgata agccagcgcc gtggcctgcg
     4501 gacggatcag cagcccttgg gcggggtcat cggtgcaata ggggcgatgt ggcagacgtt
     4561 caacaaatga ggcgtaagag gccgatttta ccatcgatca ccgcacaaat tggcggtgca
     4621 aactccacac tcttccacac aagataggtg gaatgagggg ggagttggtt gtctataaat
     4681 aaccatgtgt tagacttttc ccataggttg cgtacctagc cccgaactct cgttccgtct
     4741 tccgccaagt tgatgaaaga gattcggggt ttgtcctttc tgggcaatac gttacacacc
     4801 atcaaacaag aaatctatcg cgtccttgat gagctgacgc gactcctgca caccagtgac
     4861 ttcatcccca agctgcgcca gcctcaccgc tgccatgtcc tgcgtcatca ccaaatactt
     4921 atcaccctca acgatgatca ccggattcag ccgctgcgct ggccgtccag tgaaggctac
     4981 cagcgggaac agtgggatca ccatcctggt ggccagctcg ccaatgatgt cgctttgcac
     5041 atccagcaaa taggggtaag cgcgggcatt gcccttgttg cggtagatag tgaactgcat
     5101 caaaacgccc tgtaatcgtc ggagagcagg ccgtgctcct cggtgatgcg gttcagcgtc
     5161 tgtatggctg cactgttctc acgcttccag cgttctgctt ctgagccgct gatttcattc
     5221 tgtagcgcgg ttgcaagggt ggctgacagg ttgatccccg ccgctctggc tcgttccaac
     5281 aagctgggct ccagcgtgac gctgaccgtt ctcttgctgc gcattgctgc tgcgaccatg
     5341 atgacctcca attaagcacg tgtcgtgaca cgtgccaaca ttaacacatc cctattcaag
     5401 atcccaacaa gcgtctcgga gcaacctcaa acccggcggc atcgatctca aagcggacca
     5461 cgccgatgtc ctgcaaatag gcctgcaagg tatcgagctt acgaaagacc cggagttgtt
     5521 tgctgcgctg ggcggccagg gcacgttcgg ccagaccgta gcgcaccagc agcgtccagc
     5581 cgccaggttg tccgacggcg accacgacac gaacggcacc ggccgccacc agttgtacca
     5641 gggtgggatg ggtaatggtt tccatggggg agattccgag gcaagggata gtgcggcgca
     5701 ggatgggaga tgacgcctcg caaaacaagc catcttgcag gatgacaggc agcaaaaatg
     5761 gcgtccgatt taacgtaacg ccccccttat gcgcggttag gctcaggggg gccggaggcc
     5821 cccttggtca caaagaccgt cagaacagtg aaaccagtaa agctagtaca gatatgatca
     5881 cagcagtccc agcaagcaac agactggctg tcatactcat gcgcgaatag taaaacgtcc
     5941 tcattttctc cagcatctcg cctgatgtct ctacagcaga ctgagattca tgagcctctt
     6001 tatggatcat ttcgctataa gcttgacgga tacgggcttc aatagcctca tggacctcat
     6061 gattccagtc gtgagtcaca tccactaact ttcttacaat agtacgcttt aaggacatgt
     6121 ttttccttat tctgccagag cctcaagcat ctgttttccc ttgccaatga agtacattcg
     6181 aagggtttca tcagaaatga gattatttgc tcgcactatg atttcgatca cagaacggtt
     6241 ttcatctttg gccagtaggt acttcaagaa gaaatctgca tcggtttcat tctcaatttt
     6301 gaagcgctgg atcatattaa gctccttcga tgaaaagcac ccgcctgggt gggatattct
     6361 tgttcgttgc cgatatttgt ttgcatgaaa acgccccatt actctacggt aatagaacca
     6421 tcctgatgaa ggttgaacac aaatttttga tcaatccatt tttctaatcc cacaccaatg
     6481 ttaatttctg aaatcttgtt tcctgttaga ttctcgccgt atttgccaat aacagcccta
     6541 acattttctg gctgaaggcc agaaccgatg gtattgatcg tcgtgaggta ctttccaggt
     6601 cgtaatgctt ccccttggtc ggtaagtggg gcaaattcag caaaaccggt cggtgaaact
     6661 tcggctctat gctgggcgat atatcccgtc ccaccccagt tgatagggtt aacaacgaag
     6721 attatgaaaa ttgtaccttc aggaaggttt gttctgaccc tgacaacagg tttcccgtcg
     6781 ttaagatcca taccaacttc gaaagaaacg tcgaaaggaa cggcggtggg ctcggcttca
     6841 ttgtcgacaa gtgcagattt gctatcgtgg ctttctgggg cggttagcgc atcacctttt
     6901 ggtgtcgggg tgccaattgt tgttgggatt cccttgctgc gtatggcttc atatatagca
     6961 aacgcagaat cataccaagc agttaggcac agggtgtttt cacatctctc acgagacatc
     7021 catagcgcat ttctaatgct tgaaaatgct tgctgatctt gagttgcagc ctttgctttc
     7081 ttatataagg cagcaagtaa atcgtctttc ttggatagtt ctggtgtcga gcatatttct
     7141 ttttctgaat aactacgagc cttggagcaa tcaaaagaca cggcatgtac cgatac
//
