LOCUS       NZ_CP091118             7636 bp    DNA     circular CON 08-APR-2024
DEFINITION  Bacillus thuringiensis strain x023 plasmid unnamed8, complete
            sequence.
ACCESSION   NZ_CP091118
VERSION     NZ_CP091118.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13118470
            Assembly: GCF_021651035.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 7636)
  AUTHORS   Liu,Z., Xie,J., Deng,Z., Wang,M., Dang,D., Luo,S., Wang,Y., Sun,Y.,
            Xia,L. and Ding,X.
  TITLE     Enhancing the insecticidal activity of new Bacillus thuringiensis
            X023 by copper ions
  JOURNAL   Microb Cell Fact 19 (1), 195 (2020)
   PUBMED   33069248
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7636)
  AUTHORS   Zhu,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JAN-2022) College of Life and Science, Hunan Normal
            University, Juzizhou Street, Changsha, Hunan 410205, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v1.05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021651035.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 11:19:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,441
            CDSs (total)                      :: 6,290
            Genes (coding)                    :: 6,002
            CDSs (with protein)               :: 6,002
            Genes (RNA)                       :: 151
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 101 of 288
            Pseudo Genes (incomplete)         :: 206 of 288
            Pseudo Genes (internal stop)      :: 65 of 288
            Pseudo Genes (multiple problems)  :: 67 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091118.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7636
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="x023"
                     /isolation_source="soil"
                     /db_xref="taxon:1428"
                     /plasmid="unnamed8"
                     /geo_loc_name="China:Danxia mountain of Hengyang"
                     /collection_date="2018-05-20"
     gene            879..1163
                     /locus_tag="GFH33_RS31970"
                     /old_locus_tag="GFH33_31970"
     CDS             879..1163
                     /locus_tag="GFH33_RS31970"
                     /old_locus_tag="GFH33_31970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126403.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_000126403.1"
                     /translation="MTANTRHMETQKIERKVTKIGNSLGLTLPQEFYTTLNIKQGDVVS
                     LEINEENEQIVIKKSKKISMPEDLNPKFLESLNRTINKFDGAFKDLVNR"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            1201..1602
                     /locus_tag="GFH33_RS31975"
                     /old_locus_tag="GFH33_31975"
     CDS             1201..1602
                     /locus_tag="GFH33_RS31975"
                     /old_locus_tag="GFH33_31975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158318.1"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="WP_000158319.1"
                     /translation="MTIYITETQAIFINETLIDMYSPNEQRGVKDTGLLQSAIYRPQQT
                     VAQEDAYPTIFHKATALFESLAKNHAFYNANKRTALACLEMFLLYNEYELKMSEQESSD
                     FTVNVVEQRLSFEEILKIIKENSSKLPLN"
                     /name="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(1764..2045)
                     /locus_tag="GFH33_RS31980"
                     /old_locus_tag="GFH33_31980"
     CDS             complement(1764..2045)
                     /locus_tag="GFH33_RS31980"
                     /old_locus_tag="GFH33_31980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002026684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_003273526.1"
                     /translation="MEYFHSYGVKFIGTVKELAKKNNLTIEETCRILELTMKIDDYDRK
                     DEQLAGIGELIKEMNDQIMGLSGGLRAISERLRDMNENVVLVSLEDKE"
                     /name="hypothetical protein"
     gene            complement(2077..3282)
                     /gene="mobV"
                     /locus_tag="GFH33_RS31985"
                     /old_locus_tag="GFH33_31985"
     CDS             complement(2077..3282)
                     /gene="mobV"
                     /locus_tag="GFH33_RS31985"
                     /old_locus_tag="GFH33_31985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033080.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000033078.1"
                     /translation="MSKFMVCHMQKFKMTDVKGLQIHNQREKESHSNSDIIQERTEQNY
                     DLIHDKRKVDYKKFIQNRIDNGVVSNRAIRKDAVVCCSFMISASPDYMNSLSQAEQRRF
                     FEESVCFLKARYGEENFVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSELHDL
                     QDKIYEHVKEKGFDIERGVSSDRKHLSTQRFKAVSLQQEIEKLEQEKKEIDSRLYDLAS
                     SLDQAKSVDEIPVKEKGGFIRSKMVEIASEDFDSIKSLAKSSESLRNENRRLKNEKIKI
                     EREKDDLYKGQRFLERQVTDLKRENRGLKEANDFLKKTLERVKEMYKEKLPELAGVIGY
                     VKGSILDKMNRKFLKRHFAGDDEVKGAQKFLNHKQEHEEQQKRLKQVRRSQQKNWDQGL
                     ER"
                     /name="mobV"
     gene            complement(3693..3893)
                     /locus_tag="GFH33_RS31990"
                     /old_locus_tag="GFH33_31990"
     CDS             complement(3693..3893)
                     /locus_tag="GFH33_RS31990"
                     /old_locus_tag="GFH33_31990"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139252680.1"
                     /translation="MNIRISVVLPYGYSYSDSNRKCDCINVFGVFERIEKAEALPVKQK
                     NMFVEEVVISLQENSHRSVGR"
                     /name="hypothetical protein"
     gene            complement(4446..4673)
                     /locus_tag="GFH33_RS31995"
                     /old_locus_tag="GFH33_31995"
     CDS             complement(4446..4673)
                     /locus_tag="GFH33_RS31995"
                     /old_locus_tag="GFH33_31995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869766.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000869766.1"
                     /translation="MKVVNNFRVIMAVKNIDNVAELVRMTGVSRNSINKLWRNDNVSSL
                     RIDTLLPICEKLNVKLSDLIEYIPDDSETK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            5031..5318
                     /locus_tag="GFH33_RS32000"
                     /old_locus_tag="GFH33_32000"
     CDS             5031..5318
                     /locus_tag="GFH33_RS32000"
                     /old_locus_tag="GFH33_32000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000323412.1"
                     /translation="MAYIISKKVKGNIYFYVAQYVGTQPYYSKQYKYKCIYAIGNQKIA
                     LERIAMWLLDNNRIPKELLEIGVSINDVKYWYEKVEKTLQNYSLTNHKNT"
                     /name="hypothetical protein"
     gene            5332..5595
                     /locus_tag="GFH33_RS32005"
                     /old_locus_tag="GFH33_32005"
     CDS             5332..5595
                     /locus_tag="GFH33_RS32005"
                     /old_locus_tag="GFH33_32005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874966.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPNELIKIGISIDDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            complement(6600..7553)
                     /locus_tag="GFH33_RS32010"
                     /old_locus_tag="GFH33_32010"
     CDS             complement(6600..7553)
                     /locus_tag="GFH33_RS32010"
                     /old_locus_tag="GFH33_32010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014482202.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_236586591.1"
                     /translation="MSESGRELFKSCSTFNEFVATKDKKKKKRVKGNDCKNRFCPICAW
                     RKAGKDAVKIATMMEAIKIEEKKEFLFLTLTTPNIKADTVKSEIDRFNKAFNKLFKRRN
                     IQRSIKGYIRKLEMTYDKERFITEEMYNNKKRKAYYDSRGLKVDDHNPNYDTYNPHFHV
                     LLCVEKNYFKRKELYIKQEEWLEMWREVTDMPEITQVHIQKVELIREGNAVAEVAKYSA
                     KDYEMSVSQDVFDVFYLALKGRQLIVYGGLLKDYAKKYEDGELDKYKSTDKNEYYYRLI
                     AMWNKDLMKFEQEYQELTESEKQEYNGHLIDEMEVE"
                     /name="protein rep"
     Typing          complement(6601..7636)
                     /name="rep_cluster_617"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2081..3282)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttgtcatgt ttgatatatt agtcctatat gaattgctta aaaaagggta cacgaatccc
       61 tttttcggac ttgctccttc cttggtagtt attgttttta attcgacact aaaaatgata
      121 aatctacccg ccggaaatag caagtctttt ttttcgaaat cccttgtgta tcaagggata
      181 aaagaataca taggcatcaa tttccgtgcg gaaatagtta cctatgtatt aaagaaagaa
      241 gaaaaccgta tttttcgtgt ccgaaaaacc ataaaaaaat gcccttcaat ccacttcgtg
      301 gacaagggca ttttttcttt taaaaacaaa aattattcct atctctcagc ctcttcattt
      361 tcacttctaa gcgtttttca aacaacttat aatattttct ctttaagaat tacacatcaa
      421 tcccctatac ctcaaaaaca tatatttcag aacacgttcg aagtgttgcc atgaaaaaat
      481 cgagaaattc tcgaacttta cctgctaatt agaataacag gtaactccct ttacactttc
      541 tttgtctaaa taaaacaaat tcaagcaagg ctctacaatc ttccctatct tttgcaatga
      601 aatataaatt tgtttacata tttctaaaaa tgagtataat tgtattgacg gttaatacgt
      661 tataactgtt ataacattct aactaaacgt cataataata cagaaagact aaacacaaca
      721 aacaaaagag aaaactaaga agaccgaaaa aaccaaaagc aaacaaacaa aacaaacata
      781 aaatcacact atataattcg aaaaactcaa tacaaacaca aagtggcaat tagactacga
      841 caaaaaatag tgtggcaaat tccaaggagg aagtccaaat gacagcaaac actcgccaca
      901 tggaaacaca aaaaatcgaa cgtaaagtta ctaaaatcgg caattcttta ggtttaacat
      961 taccgcaaga attttacact actctaaata tcaaacaagg tgacgttgta agtttagaaa
     1021 ttaacgaaga aaacgaacaa atcgttataa aaaaatccaa aaaaatttct atgccagagg
     1081 atctcaatcc taagttcttg gaatcgttaa acagaaccat caataaattt gacggtgcat
     1141 ttaaagattt ggtaaacaga taattatcct tcaggcgtgg aggaaagtag gaaaaccgaa
     1201 atgacaattt atataacaga aactcaagct atttttatca atgaaacact tatcgacatg
     1261 tattcaccta atgaacaaag aggtgttaaa gatacagggt tattacaatc agcaatatac
     1321 cgtccacaac aaacagtagc acaagaggat gcatatccga ctatcttcca caaagccact
     1381 gcattatttg aatcattggc taaaaaccac gcattctata atgcaaacaa aagaacggca
     1441 ttagcttgcc tagaaatgtt tctgttatac aatgagtacg aattaaaaat gtccgaacaa
     1501 gaatcttcag atttcactgt taatgtagta gaacagagat tatcttttga agaaatctta
     1561 aaaattataa aagaaaactc atcaaaatta cctttaaatt agaaaaagac gtgggattta
     1621 accacgtctt ttttattaaa caaactatct tcaaggatgt tatacaaggc tctacaatcc
     1681 tcgctatatt atgcaaaaca agttccgcta acgctacaca tctaaagaaa aaagagactc
     1741 acacagagcc tcctaacatg ccattattct ttatcttcca aagatactaa tacaacattt
     1801 tcattcatat cacgcaaacg ttcacttata gctctcagac ctccactaag tcccataatt
     1861 tgatcattca tttccttgat aagttctcca attccagcta attgttcatc cttacgatca
     1921 taatcatcaa ttttcatcgt caattccaaa atcctacatg tctcttcaat cgttaaatta
     1981 tttttcttag ccaattcctt tacagtacct ataaacttaa ctccataact atgaaaatat
     2041 tccatccttg gttgttgatt aaataatgtt cccacactat ctctccaatc cctggtccca
     2101 atttttctgt tggcttcttc gtacttgttt taatctcttt tgttgttctt catgctcttg
     2161 tttgtgattc aagaactttt gagcaccctt tacttcatca tcaccagcaa aatgtcgttt
     2221 taaaaacttg cgattcatct tgtccagtat actcccctta acataaccaa tcacccctgc
     2281 gagttcagga agcttctcct tatacatctc tttcactcgc tccaacgttt tcttaagaaa
     2341 atcatttgct tccttcaacc ctcgattctc tcttttcaag tctgttactt gcctttctaa
     2401 aaaacgttgc cccttatata aatcatcttt ttcacgttca atcttaatct tttcattttt
     2461 aaggcgtcta ttttcgtttc taagagactc agaagactta gctaatgatt ttatactatc
     2521 gaagtcttct gaagcaatct cgaccatttt agagcgtatg aacccgcctt tttctttgac
     2581 tggaatctca tctacacttt tcgcttgatc taaagacgat gctaaatcat aaagacgact
     2641 atctatttct ttcttttcct gctctaactt ctctatttct tgttgcaaac tcaccgcttt
     2701 aaaccgctgc gtcgataaat gtttcctgtc gctagaaaca ccacgctcta tatcaaatcc
     2761 cttttccttc acatgttcat agattttatc ttgtagatca tgaagctctg atttgttttt
     2821 aaaaaatgaa tacgctgata gtttttgttt ttcattaacc ggaaccatgc ccacatgcat
     2881 atgaggtgtt ttttcatcaa catgtactga agcatataca aagttctctt ctccatatct
     2941 cgcctttaaa aaacaaacag actcttcaaa gaatcgtctc tgttccgctt gagataaaga
     3001 attcatataa tcaggactag ccgaaatcat aaacgaacaa caaacaacag catctttccg
     3061 aatcgcacga ttagatacaa caccattatc aattcgattt tgaatgaatt tcttataatc
     3121 cacttttctt ttatcatgaa taagatcata attctgttcc gttcgttctt gaataatgtc
     3181 tgaatttgaa tgactttctt tttcacgctg attatgtatt tgtaatcctt tcacatctgt
     3241 catcttgaat ttttgcatat gacaaaccat gaattttgac atgggcatcg ctccttcttc
     3301 ggtcgtagtt ccatgtaaac tgtagcacac tacagtttac tgcgtaaaca cgtgtgccct
     3361 acgggacatt tcggcgaccg ccgagccatg acaggcatgg caaaagaatt atgggctttg
     3421 cccaaaccca caagggaaac tcttcccctt gacccctttc aatcgaactc cccaacccct
     3481 caatccttga gggactccct cgccggtagg catccccaaa acatggtttt gggaagaaca
     3541 agagggtctt ggtcgaagtt acctatcaac tccttaatgc tccatcattt cgctatcgct
     3601 tcattccgtc cgcttaccgt tgacaggtac cataaatact cctccccctt ttaaaatctg
     3661 acatccatat attaaaaagt ctctttttat attcaccgcc ccaccgatct atgggaattt
     3721 tcttgtagtg aaataactac ttcctctaca aacatatttt tttgttttac aggaagagct
     3781 tcagcttttt ctattctttc aaacacccca aaaacattaa tacaatcaca ttttctatta
     3841 gaatcactat aagaatagcc atagggtaaa actacagata ttcgtatatt cattcctaga
     3901 cccattcaaa taaaaaagag cataaggaaa ccgaatccca ctttgtacaa aaagtggtga
     3961 aatagactgt gcctattggg tttctcatgc tctacttcag ggaagcgatt ttccctaaag
     4021 tatgcgtcct ctatacgttt aaaaacgttt agtatgtatt ctgcatgtgc tttgcataca
     4081 ccatgacaaa taaaaaaaga gccccacaaa taggaactct tttttcatta ctcatcaatt
     4141 ccaccatttc attttactgc attacgataa tcacacataa agttaaacaa aatcacgaaa
     4201 tatctgccta cctctcatct tccaagatgt tgacgcacct agcaagccta taatttcatt
     4261 taccatattc atgtgataac aaacagaaaa agaaagaaaa agacagtcat aaaagtttgc
     4321 cgaaatttaa aataagcata taaccctacc aaaaaccata tgcgaatata acctgttttc
     4381 cactagcaaa tattaatacc gatttcatca tacacaagct atctaaagat agaatagccc
     4441 ctaaattatt ttgtctcact atcgtctgga atatattcaa ttaaatccga tagtttaaca
     4501 tttaactttt cacagatcgg tagcaatgta tctattcgta aagatgacac attgtcgtta
     4561 cgccacaact tattgataga gttacgactt acccctgtca tcctaaccaa ctctgcaaca
     4621 ttatctatat tctttaccgc cataatcaca cggaagttat ttactacttt cattttttat
     4681 actccttttt ctcatgaaat atacatgctt ttttcttata agggcacttt tgtgcaaaga
     4741 atagttgttt tttaaccttt taaacacaac tttagcactt tataaaacat ttttaaacct
     4801 gtatggaata aaaaatcata ttatgcacta tttcatactt catttaaatt aaagttaatt
     4861 tatatacata taaggttatt tattttattt atttaaacta atttaaaacc tatttaaatt
     4921 aaaacgttca aaatatgcat tatttcatac tttatttgtt gaaaaattaa cctgataaag
     4981 ttaatattaa tcaaagtaat atagattttt aatgattgga ggtcatttct ttggcatata
     5041 taatatcaaa aaaagtaaaa ggaaacattt acttttatgt agcccaatac gttggaacac
     5101 aaccatatta ctcaaagcaa tacaaatata aatgcattta cgcaatcggc aatcaaaaaa
     5161 tagctttaga aagaatcgca atgtggctat tagataacaa ccgtatacca aaagaactac
     5221 tagaaatcgg agtatccata aacgatgtga aatattggta tgaaaaagtt gaaaaaacat
     5281 tacagaacta ctctttaact aaccataaaa acacctgaaa ggagcattac tatggcattt
     5341 ctaacaaatt acaaagcaaa cggaaaaaga tacttttatg tagaaaaata tgttggtaaa
     5401 aaaccatata cctgcaaaca aagtgaacgt atttattcga ttggaaacga aagaatcaca
     5461 ttagaaaggc tcactttatg gattttagat aacagtttca ttccaaacga attaataaag
     5521 atcggaatat caattgacga tatagagaac tggagagaaa aagtagaaaa caccataaag
     5581 agatactctt tataaataaa actaaagaaa aaagactgat attttcatca gtcttttttc
     5641 tttaactatt tatatttttt catcataaaa gggttaggta ttttaataat atatttatat
     5701 atttaataaa ggttacgcgt ggctttttga atttttcatt tgcgcacaaa agcaaaaaga
     5761 ttttaagagt aaaaaggatt aaaaatatac aggaatcacg aaataaaaaa ttcacagaaa
     5821 caacaaattc aaacataatc taaacatctt tttttatcac acaacttgtt taaaacggtg
     5881 caacttttta acgatatgaa atatctatga attaaatatg cgagcaaagt tttttcgttt
     5941 tcagttcgtc atagaggtac tatataaact ccctaatttc ggaatacaga attacagaga
     6001 aaacggaaaa caaaaatttc aaaaatcgaa aaatcaagat ttcaaattta aaaattttta
     6061 aataaaatcc cacaccaaaa atccaaaatc aaccccaaaa atgtagaatt tctattttca
     6121 aaaaacatca aaaaacgtgc ggtattttat cgatatagat atatctaaaa aaattcatgc
     6181 gcacactata ggtttttaac tcgtacagta gtaaaacgaa atcatcagaa caaaatatat
     6241 ctcgaaataa actttaaaaa taaattcaca aattcacaaa tttaaattta agaaaaaact
     6301 cttttttgtt aattctctgt tagatcataa aaaccttttg aatgctactt gctgcactca
     6361 gaaggttttc tttcaataca aaaaagagag aaccaaactt aatattaaga ctggttctct
     6421 ctttgtcatt caaatatcat caaccgcacc acatttttca catttaacct agcacttata
     6481 tgagtaatat acatcttcac catctttgac cttacttttt gaaatccgat aaattttcat
     6541 ttcttcgtta taacattccc aacaggtatg tttaacaata tcaccttttt tatacaaaac
     6601 tattcaacct ccatttcatc aatcaaatga ccgttatatt cttgtttctc actttccgtt
     6661 aactcttgat attcttgttc aaatttcatt aaatccttat tccacattgc aattaatcta
     6721 taataatact catttttatc tgtactctta tacttgtcca attcgccatc ttcgtatttt
     6781 ttagcataat cttttagcaa tccaccgtac acaattaatt gtcgaccttt taaagctaaa
     6841 taaaacacgt cgaacacatc ttgactcact gacatttcat aatcttttgc tgaatacttc
     6901 gcaacctctg caactgcatt tccttctcga attaactcta ctttttgaat atgtacttgc
     6961 gtaatctctg gcatatctgt tacttctcgc cacatttcca accattcttc ctgcttaata
     7021 taaagctctt tacgtttaaa ataatttttt tcaacacaaa gtaatacatg gaaatgagga
     7081 ttatatgtat cataattcgg gttatgatcg tctactttca atccacgaga atcataatat
     7141 gcttttctct ttttgttgtt atacatttct tcagtgatga atcgttcttt atcatacgtc
     7201 atttctagct ttctaatata acccttaata gaacgttgaa tattacgtct cttaaataat
     7261 ttattaaaag ctttattaaa tctatcaatc tcgcttttta ccgtatcagc tttaatattc
     7321 ggagttgtca atgttaagaa aagaaactct ttcttttctt caatcttgat tgcttccatc
     7381 attgttgcga ttttcaccgc gtccttccct gcttttctcc aagcacaaat cggacaaaat
     7441 cgatttttac aatcattacc ctttactcgt ttctttttct ttttatcttt cgtagccaca
     7501 aactcattaa aagtactgca cgatttaaac aattctctcc cgctctcact cattttcttc
     7561 tctataaatt cataaattct tatattctgt ttctttttag gttgtaattt accagtgatt
     7621 tcatcttgat ttttct
//
