LOCUS       NZ_CP091086             5027 bp    DNA     circular CON 09-JUN-2024
DEFINITION  Enterobacter roggenkampii strain GD21SC1505 plasmid pHN21SC1505-5,
            complete sequence.
ACCESSION   NZ_CP091086
VERSION     NZ_CP091086.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN24967284
            Assembly: GCF_023650875.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Gao,X., Lv,L. and Liu,J.
  TITLE     New plasmid-mediated RND-family efflux pump cluster tmexCD4-toprJ4
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Gao,X., Lv,L. and Liu,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JAN-2022) College of Veterinary Medicine, South China
            Agricultural University, NO. 483, Wushan Road, Guangzhou, Guangdong
            510642, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023650875.1-RS_2024_06_09
            Annotation Date                   :: 06/09/2024 05:17:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,935
            CDSs (total)                      :: 4,819
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 33 of 159
            Pseudo Genes (incomplete)         :: 110 of 159
            Pseudo Genes (internal stop)      :: 43 of 159
            Pseudo Genes (multiple problems)  :: 24 of 159
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091086.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="GD21SC1505"
                     /isolation_source="farmer market"
                     /host="environment"
                     /db_xref="taxon:1812935"
                     /plasmid="pHN21SC1505-5"
                     /geo_loc_name="China: Guangdong"
                     /collection_date="2021-07"
     gene            join(4886..5027,1..572)
                     /locus_tag="L1S38_RS24585"
                     /old_locus_tag="L1S38_24585"
     CDS             join(4886..5027,1..572)
                     /locus_tag="L1S38_RS24585"
                     /old_locus_tag="L1S38_24585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_250670800.1"
                     /translation="MKKSHMFFIICGVSFFLCGCALPEKIKAKPYDNPDYGYVVYTPKS
                     GYQEFDGIYLEGFYSSGGQYLKKCVATGPLEISKSGLPATIRYNKDDGVVYLRYPGGPT
                     MASCDIAYDVIKRDAELYISKEKEARRVEFEQVEKEKIETKKWRAEHKREAATDDALKL
                     FVTYCEALRDATGNASYIDDLRAMKHEFVGPKTNSMMYQYAVNVFHGTGGYVYCEMIVP
                     QVKERIYTLSIDPTK"
                     /name="hypothetical protein"
     gene            complement(648..1031)
                     /locus_tag="L1S38_RS24590"
                     /old_locus_tag="L1S38_24590"
     CDS             complement(648..1031)
                     /locus_tag="L1S38_RS24590"
                     /old_locus_tag="L1S38_24590"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_250670801.1"
                     /translation="MTEKCPFCGALFTVNSIGDGGICGACREPIDCPYCNKTVREERTT
                     GVFSEMLVEAPDSQLSQYLGITDDEWDDMGAELHENTGNSGEMTYNYWFTVPEGTAEEI
                     LEKTGWKIGEMINDIPVCVVDFD"
                     /name="hypothetical protein"
     gene            1369..2304
                     /locus_tag="L1S38_RS24595"
                     /old_locus_tag="L1S38_24595"
     CDS             1369..2304
                     /locus_tag="L1S38_RS24595"
                     /old_locus_tag="L1S38_24595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_250670802.1"
                     /translation="MKCAQLELFRDRLPRKPYYSDELTTGLRIADVARALGARYIQPNG
                     PTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNAA
                     PLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSPY
                     NGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPAWLAACVDRASGYNAQFEQPLP
                     ANEVRHTAKSIAKWTHQHLSLAGFREEQARRGAKGGKVSKRPTQTGKTKADLLPDVLRL
                     KAQGFTNRDIADDLQISPSTVSVYLKRNRP"
                     /name="replication initiation protein"
     gene            2710..3102
                     /locus_tag="L1S38_RS24600"
                     /old_locus_tag="L1S38_24600"
     CDS             2710..3102
                     /locus_tag="L1S38_RS24600"
                     /old_locus_tag="L1S38_24600"
                     /inference="COORDINATES: protein motif:HMM:NF037437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_250670798.1"
                     /translation="MSITQIEKDSRRKAVNGVISSFQLEGKAITDEFKILLEDYINGKI
                     STDEMLAMVSGEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="hypothetical protein"
     gene            3084..3422
                     /locus_tag="L1S38_RS24605"
                     /old_locus_tag="L1S38_24605"
     CDS             3084..3422
                     /locus_tag="L1S38_RS24605"
                     /old_locus_tag="L1S38_24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3403..4866
                     /locus_tag="L1S38_RS24610"
                     /old_locus_tag="L1S38_24610"
     CDS             3403..4866
                     /locus_tag="L1S38_RS24610"
                     /old_locus_tag="L1S38_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016786983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_250670799.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQDSN
                     RHLRAGWREEASLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAA
                     AQGMAGKLRELGEHVREHQARGREQPGSGETLDVASGRLNAASGRLEQATGGLDRASQQ
                     VAKEKRRGLIPSDDWGLSSGKQGGYRFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          2035..2304
                     /name="ColKP3"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.143"
                     /coverage="100.0"
                     /evalue="1.23e-81"
     Typing          3404..4836
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.042"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 aagagtttga tgggatttat ctggagggtt tttattcttc tggtggtcag tatcttaaaa
       61 aatgcgtagc gacagggccg ctggaaatat ctaaatcagg gcttcctgct actattcgat
      121 acaataaaga tgacggtgtc gtatacctaa gatatccagg cggcccgaca atggcgagct
      181 gcgatattgc atatgacgta ataaagcgag atgccgagct ctatatttcg aaagaaaaag
      241 aagctcgccg agttgaattt gaacaagtag aaaaagaaaa aatagaaacc aagaaatgga
      301 gggctgaaca taagagggag gctgcaacag atgatgcctt gaagctgttc gttacctact
      361 gtgaggcatt gcgtgacgcc acagggaacg cttcatacat tgatgacctt cgtgcaatga
      421 agcatgagtt tgtaggacca aagactaact cgatgatgta tcagtatgca gttaatgtat
      481 ttcacggcac aggcggatat gtgtattgcg agatgatcgt gccgcaagtt aaggaacgta
      541 tctacacatt gtcgatagac ccaacgaagt aagattaaca ttaaaaaatg aatacggcct
      601 ggaacttcag cagttccagg ccaatcactt aaatccaaaa ttcattttta atcaaaatca
      661 acaacgcaaa cagggatgtc gttgatcatt tccccgattt tccaccctgt tttttctaat
      721 atttcctcgg ctgtcccttc tggtacggta aaccaatagt tataagtcat ttcgccacta
      781 ttcccagtat tttcatggag ctctgccccc atatcatccc attcatcatc tgtaattcct
      841 aggtactgcg aaagttgact gtctggtgct tccacaagca tttcactgaa aacccctgtc
      901 gttcgttctt ctcttactgt tttgttgcaa taagggcaat caataggttc acgacaagcg
      961 ccgcaaatcc cgccatctcc gatactgttg acagtaaaaa gagcgccaca aaaaggacat
     1021 ttttcggtca tgagtaaacc tatttgaagt ggatgttaat tgaataataa caccttgttg
     1081 tctgacgtac tcgtcatggg gctgggcagt gaaaacggcg aaaaatttat ggtgtctgca
     1141 ttactttgag gtggtttttg gatgatgcca tcagttctat atagggagtg tacgaagagg
     1201 actatttccg gtaacttact gtgcagaaag aacaaacccc cgttgctacg cagttctatc
     1261 ttggcggaga gaacgcagct aacgggggcc gtgacgaatc acaagagaag tctatcacat
     1321 ggatcatgcc aaacaaccct gcgccgccaa taagcgcggt ggttcttgat aaagtgcgca
     1381 cagcttgagc tattcaggga tcgtcttccc cggaaaccct actattccga cgagcttaca
     1441 acgggcctac ggattgcgga tgttgcccgc gcccttggtg cgcgttacat ccagccgaac
     1501 gggccaacgc accgccactg gatcgttttt gacgtagacc atgccgccgc tacgttatct
     1561 tgggatgacg taggcgcacc cgcgccgaat attaccgtca cgaacaaggc caacggccac
     1621 gcgcatctga tctacggact ggatacgccg atccgcaccg ccccggatgg aaatgcagct
     1681 cctctacggt atgccgctgc cattgaggcc gcgctcaggg agaaattagg ggcggatatg
     1741 ggctactccg ggttgatctg caagaacccc ctgcatgagc actggctggt gcaggtatgg
     1801 gagccccggc tctacgattt agcctggcta tccgattacc tcgatctgag cccctacaat
     1861 ggccgcaaat cgcttccaga gtacgggctt gggcgaaact gtaccctgtt cgagaaaacg
     1921 cgcctgtggg cctataaagc gatccggcag ggctggccgg actatcccgc atggttggcc
     1981 gcttgtgttg atcgggcatc aggatataac gcccagttcg agcagccgct ccccgcaaac
     2041 gaagtccggc acacggccaa gtccattgcc aaatggacgc accagcacct aagccttgca
     2101 gggttccgag aagagcaggc ccggagaggt gcgaagggcg gcaaggtatc caagcgccca
     2161 actcagacag gcaagaccaa agctgatctt ctcccggatg ttctgcggct taaagcacag
     2221 ggattcacca accgcgacat agcggacgat cttcaaatct cccccagtac ggtgagcgtt
     2281 tacctaaagc gtaatcgtcc gtgatcgaat aagccttatc agataatagc ccccgttagg
     2341 gggttcctgg gctcttgtct tttagcctta aaaaatcctt ttagcggagt gatctggcga
     2401 ggtgggtaac tgcgcccgtc cgtccatcgc ccccctgttt tttcgggggg tgtgggcggc
     2461 agcagttatc cacggagcag ctcttgccct tgatcttgac ctagggcttg cagtatccct
     2521 cctatggtcg gatactgtac taagcaaggc tacacagagg cccctctacc tgcttcgcgg
     2581 tctcgggcct ctgtggcctt gttcaggggt gctacgcccc cccgaaaccc cctgtgtccc
     2641 ccgctcgatc cgccccttcg ggacggtctg cgcggtggct tcttggctct ggataatgga
     2701 cgatgcccta tgtcaataac acagatagag aaagattcga gaaggaaggc agtcaatggc
     2761 gttatttcta gtttccaact agaggggaag gccataaccg atgaattcaa aattctactt
     2821 gaagattata tcaatggaaa aatttccacc gatgagatgt tagctatggt aagtggcgaa
     2881 cccacaacaa cggaatacga ctacgaaatc accccccgca ctctcggtct tggcgggggc
     2941 tggaatctgc gtttgctgga gaacggcgaa gaggtcggcg ggggtgtttt ccccttgccg
     3001 gagcactgcg attttcggga tgaaaaggcg ctccagacgc tactcgatag cctctatgag
     3061 gacgccttag cggaggcctc tgcatggctg gccagccgtt aaagcgcctc cgctctatca
     3121 agatccgggt ctcagatgcg gagcttgagc gcctgcggga gctctgcccc aaggcccaac
     3181 tggcggagtg gatgcgggag caatgtctcg gcgttgcgca accccagcgc cgaacgccgg
     3241 cgcctaccgt agatccggcg ctcttgcggc agttggccgg aatgggcaac aacctgaacc
     3301 agatcgcccg gcgcgttaat tccggggagt gggggccgtt ggatcggctg cgcatcatag
     3361 cggagctgtc cgctataggc cgcgagctgg aggaactgca ccatgatcat caaattccat
     3421 aaccgtgggg taggccgggg ctccgggcct gttggctact tgttgggccg tgacgggaac
     3481 cgggaaggcg caacgctcga ccggggcaac cctgcggtga ttgaagccct gattgattcc
     3541 agtccctacg ccaaaaaata cacctctggc gttctgtcgt ttgccgaggc tgatttaccc
     3601 agggcgacca aggacaagct gatgagcgac tttgaaaagg cgctcctgcc cggactggag
     3661 ggcaaccagt acgctgtttt gtgggtagag caccgcgaca aggggcggct ggagctgaat
     3721 tttgtagtcc cgaacatcga attgcagagc gggaagcggc tgcaacccta cttccacaag
     3781 gccgacaatc cgcgcctcga tgcgtggcga accgtggtaa acggggagct gaaattgcac
     3841 gacccggacg atccgttaaa ccgtcaggcc gttatcacgc caaaggattt gccgaggggg
     3901 cacaaggagg ccgctcaggc cattacagac gggcttttac gcatggccgg agcaggggag
     3961 atccagagcc gcgatgatgt gcgcaaagcc ttgcagggtg cgggtttcag cgtggtacgc
     4021 gaaaccaaaa acagcctgag cattgccgac ccggacgggg ggcggaacat ccgattaaag
     4081 ggggtgctgt atgagcaaga ttttagacat ggcgaaggac ttcgagcaga actcgaagca
     4141 gcaggccaac gctatcgaga agcgtctgac cgaagaattc acgaggcacg agaaagctat
     4201 ctccggggcg ttgaaatcaa gcgaggagag aatgagcgcc gctattgccg agcacagcca
     4261 gaaaatgggc tggctgttgc tcaaaacgtg gatctggatg ctggtcagca tcgtcctgtt
     4321 cttgagcgca acgagcggga tcctctggta tcagggggaa ttgatagcgg agcggcaagc
     4381 cactctccaa gccttggcag agaagggcgg caagattcaa atcggcactt gcgggctgga
     4441 tggcgagaag aagcgtctct gcgtgcagat ctccgacccc aatccaaccg tttgggggaa
     4501 ggatacctat tggaccgtgc cgaaggggta ctaaatgacg ggattggagc gggaattgct
     4561 ctgcgcgttc gaggctttgc agagcgcata cgagcagcag cacaaggcat ggcaggaaag
     4621 ctacgcgagc ttggcgagca tgtacgagag caccaagcga gaggccgaga gcagccggga
     4681 agcggcgaaa cgcttgacgt ggcaagtgga cggcttaacg cagcaagtgg acggcttgag
     4741 caagcaactg gagggcttga ccgggctagt cagcaagttg caaaagaaaa acgccggggg
     4801 ctgatcccta gtgatgattg gggcttgtca tctggcaaac agggcggcta taggtttgaa
     4861 cgataaaacg aattggatat gccacatgaa aaaatcacac atgtttttca ttatctgcgg
     4921 tgttagcttc tttttatgtg gctgcgcatt gcctgaaaaa ataaaagcaa aaccatatga
     4981 taatcctgat tatgggtatg ttgtgtacac gcctaaatct ggctatc
//
