LOCUS       NZ_CP091073             5596 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Klebsiella pneumoniae strain WSCRKP plasmid pWSCRKP-5, complete
            sequence.
ACCESSION   NZ_CP091073
VERSION     NZ_CP091073.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN24836900
            Assembly: GCF_021560095.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Zhou,Y.
  TITLE     Klebsiella pneumoniae isolated from patients with pancreatitis in
            Wuxi, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Zhou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2022) foundation medicine, Southwest medical
            university, Longmatan District, Luzhou, Sichuan 646607, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SOAPdenovo v. 2.04; unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio Sequel; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 05:09:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,923
            CDSs (total)                      :: 5,801
            Genes (coding)                    :: 5,625
            CDSs (with protein)               :: 5,625
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 67 of 176
            Pseudo Genes (incomplete)         :: 119 of 176
            Pseudo Genes (internal stop)      :: 23 of 176
            Pseudo Genes (multiple problems)  :: 33 of 176
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091073.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WSCRKP"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pWSCRKP-5"
                     /geo_loc_name="China:Wuxi"
                     /collection_date="2018-01-01"
     gene            complement(98..343)
                     /locus_tag="L0980_RS29420"
                     /old_locus_tag="L0980_29420"
     CDS             complement(98..343)
                     /locus_tag="L0980_RS29420"
                     /old_locus_tag="L0980_29420"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     gene            594..1103
                     /locus_tag="L0980_RS29425"
                     /old_locus_tag="L0980_29425"
     CDS             594..1103
                     /locus_tag="L0980_RS29425"
                     /old_locus_tag="L0980_29425"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            1110..1322
                     /locus_tag="L0980_RS29430"
                     /old_locus_tag="L0980_29430"
     CDS             1110..1322
                     /locus_tag="L0980_RS29430"
                     /old_locus_tag="L0980_29430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1989..2213
                     /locus_tag="L0980_RS29435"
                     /old_locus_tag="L0980_29435"
     CDS             1989..2213
                     /locus_tag="L0980_RS29435"
                     /old_locus_tag="L0980_29435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            2197..2463
                     /locus_tag="L0980_RS29440"
                     /old_locus_tag="L0980_29440"
     CDS             2197..2463
                     /locus_tag="L0980_RS29440"
                     /old_locus_tag="L0980_29440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2533..2883)
                     /locus_tag="L0980_RS29445"
                     /old_locus_tag="L0980_29445"
     CDS             complement(2533..2883)
                     /locus_tag="L0980_RS29445"
                     /old_locus_tag="L0980_29445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2949..3494)
                     /locus_tag="L0980_RS29450"
                     /old_locus_tag="L0980_29450"
     CDS             complement(2949..3494)
                     /locus_tag="L0980_RS29450"
                     /old_locus_tag="L0980_29450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(3520..3846)
                     /locus_tag="L0980_RS29455"
                     /old_locus_tag="L0980_29455"
     CDS             complement(3520..3846)
                     /locus_tag="L0980_RS29455"
                     /old_locus_tag="L0980_29455"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(4204..4362)
                     /locus_tag="L0980_RS29655"
     CDS             complement(4204..4362)
                     /locus_tag="L0980_RS29655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            5002..5178
                     /locus_tag="L0980_RS29460"
                     /old_locus_tag="L0980_29460"
     CDS             5002..5178
                     /locus_tag="L0980_RS29460"
                     /old_locus_tag="L0980_29460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            5168..5512
                     /locus_tag="L0980_RS29465"
                     /old_locus_tag="L0980_29465"
     CDS             5168..5512
                     /locus_tag="L0980_RS29465"
                     /old_locus_tag="L0980_29465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          4837..4916
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(4254..4651)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          3..1438
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.791"
                     /coverage="94.0"
                     /evalue="0.0"
ORIGIN
        1 cctgcaggga aagccggagg aggtccggga gcttatcgac gcctcgccct acgccaaaaa
       61 gtacacctcc ggcgtcctgt cttttgctga acaggattta ccgcccggcc agcgtgaaaa
      121 gctgatggcg agcttcgagc gggttctgat gcccggactc gataaagacc agtacagcgt
      181 gctgtgggtt gaacaccggg acaaggggcg gctggaactg aattttctgg taccgaacac
      241 ggaactgctg accggcaggc gtctccagcc gtactacgac cgggcagacc gtccgcgcat
      301 cgatgcgtgg cagaccgtgg tgaacgggcg actggggctg cacgacccga acgcaccgga
      361 gaaccggcgc gcgctggtga cgccatcttc gttacccaaa acgaagcagg aagccgcaga
      421 ggctattacg cggggcttac tggccctcgc ctcgtcaggg gagcttaaaa cgcgtcagga
      481 cgtcactgag gcgctggaaa gcgcaggttt tgaggtcgtg cgcaccacaa agagcagcat
      541 cagcattgcc gacccggacg gagggcgaaa catccgactt aagggagcca tctatgaaca
      601 gtcttttaac gctggcgaag gacttagagc agaaatcgaa agcgcagcag cagagtaccg
      661 gcgaaatgct gaaagccgca ttcagcgagc acgagaagtc tgtcagagcg gaactgagcg
      721 aaagcgggaa gagaatcagc gccgccatcc gcgcccacga gcagggtatg agcgcggcca
      781 tgcagtcgaa ccgcctgagc gtgatgcgta tggtcgggcg gacatggctg accatcacga
      841 tggtgtcagg actgctgttc gccagcctga gcggggtgtt gtggtatcag gggagcttga
      901 tagcgtcaaa tctggccgaa atcgaccggc agaacgcagc gctgtcgaag ctgaacgcga
      961 agacctgggg cgtgacgtac ctggaagaca gcaacggacg ttttctggtg ctgccgaagg
     1021 ggacggccgt agacaggacg cagagctgga cggtcgggaa cgggcggtcg aagcagaacg
     1081 cgctgagact ggtgaaggag taagtgccga tgaccgagct ggaaaaacag ttgctgagcg
     1141 cattagagca gctacagcag gactactcga aaaggctgga cgagtgggag agcgccttcg
     1201 gggaatggcg gaaaatgtca gggcttatgc aacgggagaa cgcggcgctg agcgagcgcg
     1261 tgacgcgctt gagtcagcag gtggaacgct tgagcgggca actgcagcgt ttgagccggt
     1321 agtgcagcgt catgagatgg ccgtggccgc agaacgggcg cacgagcaac ggcagcacga
     1381 aaaggaactg acggaagcgc gttcccggca gcgaagctat gacggtccgt cgctgggctg
     1441 aaatgcagga aaacagggtt ctcatcgggc atttacgctg aaaccggatg tcattcacct
     1501 ggctgcatgg ctatgcagcc aggtaaaatt tatctgtcgc gaacggggcg gtttatcggg
     1561 tggtttgttg ccggttttac ctgactgcca ccccgttcgc ggcgaacccg tccggggcgg
     1621 tgcgggcaac gggtgttatg tttaatcagc gtgaacggca gatacaaaaa agccccggag
     1681 ggcttttaac tactgactta tttttttgaa gatttgctgg gtttcaattt gtcagtattt
     1741 cccggcgttt ttttattgtc acgctggcgt ttatcaggtt cccctgttga atcggcaatt
     1801 cgtttcggac ccggcttaag acccataatc gttatccttt caatagttaa gaggaaagcc
     1861 ctcaagctag atatattgac cgcgttacgg attatcaaga ggggctaaca agattatttt
     1921 ttgttctttt aggttgattg tggtactgtt gatatacatg tatagcaaat ggtgttcggg
     1981 aggtttatat gcttgctatc cggttatctg atgagattga gtcccgtctg gactcgctgg
     2041 cgaagcaaac cggcagaaca aagacgtttt atgcgcggga agcaatactg gcgcatctgg
     2101 aagacctgga ggattattat ctttcagcag aaactgctgc acgcgttcgc cgtggtgatg
     2161 aagcagtgca ttcgtctgaa gacgtgagga agtcacttgg tctggacgat taactattcc
     2221 gatcgggcgc tcaaatcgtt acgcaagatg gacaaacaga acgcacgacg gattgtggat
     2281 tttatgagtt tacgcattgc agttgctgcc gatcctcgcc agtcagggaa gccgctcaaa
     2341 ggtgagctgg gcgagttctg gcgctatcgg gtgggagatt atcgcgttct gtgtgagatc
     2401 cgagatgacg agcttgttat ccttgccgcc acgattggac atcgccgcga agtttacgac
     2461 tgaagcccgc tatgcgggct tttttgatgc cgacaacagg tattatggta aatactcgac
     2521 taactacgtg ttctactgtt tgtcggtttt agttaaccag caggttgtct taacgataat
     2581 gtagccgtta tttttaacta ataaggtcat attgttaacg tcaggcataa taaaagacat
     2641 tatggcctca gagttatcat cagaagcagc tctctgcatt actacttcga ttcccttttc
     2701 tttgaatcta taagtatcca gttgcctatt aaacttagaa agacctacaa ccatacttag
     2761 tttgttatct ttaacagtaa gagatgcttc ctgagtacca ttacatcggt agtaagcggt
     2821 gtaaccgtca gcaaaagatc gaccggggaa tataaatgct aggaacgcaa ttaaccaata
     2881 catagttgag aatcttggta ttacacattt cactatcctc tctttcattt ttcgttcacc
     2941 atactttgtt atctgatttt taatttttct attttataaa ccaagtctct accaaaaagg
     3001 cgaaaattat cctcaaataa taataattgt ttaagaacat ccatgaaagc agctgcttca
     3061 ccctcagtta tgggagctac tctatataac atataacctg agttaagggt ttcttttata
     3121 ctttcttcaa tgctcattat aaatattagt ggattatcat ttttcttccc ccatgggttt
     3181 ctaattaatc cttcgatgta ctcaggtcct ttgcgtccta ttgcctgcgc aatatttttt
     3241 atttcatctg cgtgagggct ttgttgtaca gctttgttta tgcctgcttg agtgattttc
     3301 agagactcta caccgtgtat gttgtatgag ataaaatgta ataaattaag atgcaatgca
     3361 attaatccat ttttgtttac atctgagtat ttggataaaa aacgtaatga agtgttaact
     3421 ctcccttgaa gcctaacaat tgggagtgct gccttgtata tttccttctc cgttaaatac
     3481 cgtttaaaaa acataattct ccgcctccgt atagcgtgcc tattttattt cgtcgtaaaa
     3541 cttagctctg aaatattcct ttagaaccat catgtcatta ataattgaat ctatctcatc
     3601 cattttttct tggtattctt ttaatggtga agggagcggt aactcaatat ttgaaaagtg
     3661 attggcgaga tagggatcaa ggtttctcat ccattcaggc ttttcgccaa tgcgttcttc
     3721 aactgaaagt gaacctgata atctctggtt aataagatca cttagactca ttttggtttt
     3781 attcgaaagc cgttgtaagg attgctttaa tcctatatct agcatgatat ttgttctttc
     3841 tctcattacc cccccttgct attgcgtttt ttgttgcgca caaagctacc attgcgcatt
     3901 aagaaacgca ataaaaaagg agggagggag tggtttgtac atgatgtgca gtgcgctgag
     3961 ggcatcagtg gcgggtgttt ttccgtggga tggatggagc tccggggata acagaactga
     4021 cgatcattta aaggatcttc ttgagatcct ttttttctgc gcgtaatctc ttgcactgta
     4081 aacgaaaaaa ccgcctgggg aggcggtttg atcgaaggtt aagtcagttg gggaactgct
     4141 taaccgggta actgggtata cgagaccgca gataccaaaa ctgttctttc agtgtagccg
     4201 cagttaggca cccacttcaa gacctctcaa tatctctctc gtatatatcc agttaccaat
     4261 ggctgctgcc agtggcgttt ttgcgtgtcc gtccgggttg gactcaagat gatagttacc
     4321 ggaaagggcg cagcagtcgg gctgaacggg gggttcgtgc atacagccca gcttggagcg
     4381 aacgacctaa accgagccga gataccagca gcgtgagcta tgagaaagcg ccacgcttcc
     4441 cgaaagggaa aaaggcggaa caggtatccg gtaaacggca gggtcggaac aggagagcgc
     4501 acgagggagc cgccggagag aaatcaccgg catctttaag tcctgtcggg tttcgtcctc
     4561 ctctgacttg agcgtccatt tctgtgatgc tcgtcagggg aggcggagcc tatggaaaaa
     4621 cggcaacgcc gcatcctttt ctccggccct ttctgatttg agcgcctttg ctttatcccg
     4681 ttatccgtga gtcggtcacg cccgccgcag ccgaacggca ggagcgcagc taccgagtga
     4741 gcgaggaggc gtcatttcat ctgtccgtgc atggtattgc ggacgctcct gagccacata
     4801 tcacacctgc cacatgaggc actttcatga atcgtgctct catgccaaca tagtaagcca
     4861 gtatacacag cgtaaacgct gtgactggtt cagggctgcg ccccgaaacc cgctaacacc
     4921 actgacgcgc tggcgcttgt ccaacgccgg agcgacccgg aaaacttttc cgtgccgtcc
     4981 ggcgtttgcg aaggagggat aatgcagaag ccgaagaaga agccgctcgt cgtaaaaaaa
     5041 agatcctggc atccggcagt gatggcaaat ctgatgcgtc aggcacagga ggaagattac
     5101 cgccgggagc agatggcaga gcttgagcgt tcagcccggt cacattttgc ggatatcgga
     5161 ggagaggatg gcggttaagc gtacccgttt tttaggtatc cgggtgaccg atggggagta
     5221 ccagcagctg ctggagcgct gcaatggaag gcaactggcg gtgtggatgc gggaaacctg
     5281 tctcgatacg cgcccggcgc ggtcgttgcg gctgccgtcc atcgatccgg ttttgctgcg
     5341 ccagcttgcc gggatgggga acaacctcaa ccagatcgcc cggaaaatta acggcggcca
     5401 gtggtcaggc gctgaccggg ttcaggtggt ggccgcgctg atggccattg atgccgaact
     5461 cgagcggctg cggcatgccg tgctggaaaa gggggcagac gatgatcgtt aagtttcatc
     5521 cgcgagggcg aggcggcggt gccgggccgg tggattatct gctggggaaa gaccgccagc
     5581 gcgacggggc catcgt
//
