LOCUS       NZ_CP090577             8446 bp    DNA     circular CON 27-JAN-2024
DEFINITION  Staphylococcus epidermidis strain 11H plasmid pSE11H-2, complete
            sequence.
ACCESSION   NZ_CP090577
VERSION     NZ_CP090577.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18740291
            Assembly: GCF_021484865.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8446)
  AUTHORS   Morand,S.C.
  TITLE     Staphylococcus epidermidis strain:11H complete genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8446)
  AUTHORS   Morand,S.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2022) Advanced Research, L'Oreal, 1 Avenue Eugene
            Schueller, Aulnay-Sous-Bois 93600, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2022
            Assembly Method :: MaSuRCA v. 3.3.0; Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1389.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/27/2024 17:01:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,185
            CDSs (with protein)               :: 2,185
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 29 of 51
            Pseudo Genes (incomplete)         :: 32 of 51
            Pseudo Genes (internal stop)      :: 18 of 51
            Pseudo Genes (multiple problems)  :: 22 of 51
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP090577.1.
            Bacteria and source DNA available from L'Oreal.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8446
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="11H"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="pSE11H-2"
                     /geo_loc_name="France"
                     /collection_date="2013-04"
     gene            complement(470..1678)
                     /gene="mobV"
                     /locus_tag="KB225_RS11540"
                     /old_locus_tag="KB225_11540"
     CDS             complement(470..1678)
                     /gene="mobV"
                     /locus_tag="KB225_RS11540"
                     /old_locus_tag="KB225_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002958641.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_234773850.1"
                     /translation="MKLAWNTQKNKMSDVKGKEMEQERKGRIKNDEIDLKRTNLNFDLV
                     EDERHLYHRVKDRVDYYKEQGSRVQKNSVVMYSNIITLSKEEADRMGKTRTKHYFKTCK
                     DYFSERFGEANVVSAKVHMDESAPHMHLHFIPVNHQGRLSARTAMNRQAINHIHDELTT
                     HLCQQGFDVERGSTDDNKTYIQDIHEYKKKAKVLSELDQQIETKKQLLRDINDLHDEKE
                     DLTRDIQNLKRKNHFLQEDSQQAQEDIDLYKQLRTISRDDFKKDEEKYQKEIKAYEASL
                     SGLRMNYRDLDKRNQHLTTQNNQLEHHLDMLNARHQAMNDMVRNDTRILFAGLRCALKD
                     RNIELSAEIKYQRMPEVGIDPDTVDQGALTHAYQGDVSGIDHHIRKASAPIKKETENDL
                     ELE"
                     /name="mobV"
     gene            2133..2552
                     /locus_tag="KB225_RS11545"
                     /old_locus_tag="KB225_11545"
     CDS             2133..2552
                     /locus_tag="KB225_RS11545"
                     /old_locus_tag="KB225_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002511307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002498221.1"
                     /translation="MPKKQNSKEKILTIRVSQSQYEKICALADIREMNVTEYIRHTAIG
                     NRIKPTVIEQIKESSEVSELFKKDNEALKSNNQALQQHIHQLEDEIDQLRQENDVFHHL
                     LQHFDSTAFLDFSTYRDDRPLKNAIKRLKEQYKSK"
                     /name="hypothetical protein"
     gene            complement(2725..3003)
                     /locus_tag="KB225_RS11550"
                     /old_locus_tag="KB225_11550"
     CDS             complement(2725..3003)
                     /locus_tag="KB225_RS11550"
                     /old_locus_tag="KB225_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002498219.1"
                     /translation="MLLKETEDYEKYYKIGNAVMGAAFICIVIFSLMKSTFLSDLSFLK
                     YIHFASEVLLIAGLIINSVPDFLTWNIKNIIFDLIIISFLIVFFIWV"
                     /name="hypothetical protein"
     gene            complement(3100..3900)
                     /locus_tag="KB225_RS11555"
                     /old_locus_tag="KB225_11555"
     CDS             complement(3100..3900)
                     /locus_tag="KB225_RS11555"
                     /old_locus_tag="KB225_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498218.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="WP_002498218.1"
                     /translation="MKSSLFILKEHFNNLYLMKRLAQFEMKISNKNNYLGTAWELINPS
                     IQIFMYWFIFGLGFRNNRTVENIPFIFWLIVGISMWFFVNQGILNGTKAVTSKYKQVAK
                     MNFPLSILSSYIVLSKFYTHVLLLILIMLICALNGYYPTIYTLQLLIFIPYTLLLTMTI
                     TLLTSTLAVIIRDIQMLMQALMRIIFFISSILYLPTNALVLKVIQFNPIYFIAESYRAA
                     ILYQNWFFITHWKLSLYNLAFLLITFVLGSILHVKYREQFADFL"
                     /name="ABC transporter permease"
     gene            complement(3996..4199)
                     /locus_tag="KB225_RS11560"
                     /old_locus_tag="KB225_11560"
     CDS             complement(3996..4199)
                     /locus_tag="KB225_RS11560"
                     /old_locus_tag="KB225_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032605129.1"
                     /translation="MNRILKISKLLIPFSLVTTLSYASASTGFMNDNSHEAFAKSKKKK
                     HKGNGNKHNCFPVGNSEFCTTP"
                     /name="hypothetical protein"
     gene            complement(4757..5323)
                     /gene="lepB"
                     /locus_tag="KB225_RS11565"
                     /old_locus_tag="KB225_11565"
     CDS             complement(4757..5323)
                     /gene="lepB"
                     /locus_tag="KB225_RS11565"
                     /old_locus_tag="KB225_11565"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503824.1"
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009306 - protein secretion [Evidence IEA];
                     GO:0006465 - signal peptide processing [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="WP_046593859.1"
                     /translation="MKKEFKEWLIAIIVAILLLFLVNNFLFKPYTVSGLSMYPTFNNKD
                     KVVVSKISKSLNHLKSGDVIIFHQNSNNDFIKRLIGKPGDQIEYKNDKLYINKNYIKEP
                     YLDYNKKMNNSSENLTEDFNVSNIKGSKNKMIIPKDKYLVLGDNRTNSIDSRYSEVGLI
                     SKKQIVGKVILRFWPFNDIKYNFKR"
                     /name="lepB"
     gene            complement(5865..6476)
                     /locus_tag="KB225_RS11575"
                     /old_locus_tag="KB225_11575"
     CDS             complement(5865..6476)
                     /locus_tag="KB225_RS11575"
                     /old_locus_tag="KB225_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="WP_046593858.1"
                     /translation="MKKVYIFLLLIFSVILVSCSNSNNDNHIEKKNEEKIVEKPLPKRI
                     FNKKTNNQYITENEMKKSIKLYLDKDEELSESSEYYEDKVDSEESLSQSEIKNLKHLNS
                     LRQKNDMNFKNYIINNKLPKDYKKGTERISKYISHSNRYSKNLEKKLDQIINKSTKHEK
                     VSTKDIGEIKNDSKIVNGREQSKIEKFLQEKNIKTKAFKK"
                     /name="NDxxF motif lipoprotein"
     gene            complement(6473..7042)
                     /locus_tag="KB225_RS11580"
                     /old_locus_tag="KB225_11580"
     CDS             complement(6473..7042)
                     /locus_tag="KB225_RS11580"
                     /old_locus_tag="KB225_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="WP_046593857.1"
                     /translation="MNKEEYLKIIQKETKKINTDEKEDILSKYESYFINGYKNNKNDEE
                     IIKELGDPVKIAKEINAVNSLYKVENKKSVKSIFSAAFSIMELSILNLILIIFSFFVLL
                     LFSPFILAYFIAVPIMIISPIILLVMGIINGFSTINTGDIIEVIKGVTLGIILVFIGYF
                     ITKYFWKVLVNYLNWNITIYKKGKMK"
                     /name="DUF1700 domain-containing protein"
     gene            complement(7381..8244)
                     /locus_tag="KB225_RS11585"
                     /old_locus_tag="KB225_11585"
     CDS             complement(7381..8244)
                     /locus_tag="KB225_RS11585"
                     /old_locus_tag="KB225_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440631.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_046593856.1"
                     /translation="MSGETVIYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLHL
                     SFDELKELSNYNPETRNINRFVDDLQKVYDKMLDIRYTIRTETKIKKFVLFYKYEIDIS
                     ERYLEIATSPDLKYILNSITNNFTKFELKEMTHLKSTYAKNMFRMLKQYKHSGYMKIRV
                     EDFRERLDIPNSYRMSNINQFVLTPIIRELSTIFNNLKINKIKAKKGRKIEWLEFIFDP
                     EKRIHSKRQTQMANIGKSRQYISREKTPKWLEDRTYEKTTQNEYDPQLEKEREAFLKQL
                     QVDWEE"
                     /name="RepB family plasmid replication initiator protein"
     Typing          complement(7382..8244)
                     /name="rep_cluster_1448"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.896"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(474..1750)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.592"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggcaaaact atgcctttaa catttcaaaa gccttgtccc tctagatctt aaacagtact
       61 taaaaaggtt ttaaaaagat ttataaaaat atattaaaaa ggtcggagtg aaatacttca
      121 aacgcattca aatatattta taaagaaaac aacacaaaaa taaactttta aaagaatgga
      181 ggtatttttt aaagtttaga aacccaaaat atcttcatca attaatttag aaatcatact
      241 cacatctatc acttccaata caatattttt gccaccttgg aattcttata ataatataga
      301 aatgcaaagg tatcgttcct aattattgaa ctagtttact aattcagtaa ttaaagttat
      361 tattgctctt aataagtgtc gccagcttat taagttttta cttactcttt tcattgagat
      421 aagcgctcaa acaagtatca tacttgcctg agtgcttatt tttattgatt tattctaatt
      481 ctaagtcatt ctctgtctct tttttaatag gtgctgatgc ctttcttata tgatggtcta
      541 tccctgacac atcaccttga tatgcatgtg ttaaagcccc ctgatccacc gtatcgggat
      601 caataccgac ttctggcata cgttgatatt taatttctgc gcttaattca atgttcctat
      661 ctttcaatgc acatctaagt cccgcaaaca aaatacgtgt atcatttctt accatgtcgt
      721 tcatcgcttg atgacgtgca tttaacatgt ctaaatggtg ttcgagttga ttattttgtg
      781 ttgttaaatg ttgattgcgc ttgtccaaat ctcggtaatt cattctaagg ccactcagag
      841 acgcctcata cgctttgatt tctttttggt acttttcctc atctttttta aaatcgtccc
      901 tagaaatcgt tctcagttgc ttgtataggt cgatatcttc ttgagcctgt tgagaatcct
      961 cttgtaaaaa atgatttttc cgttttaagt tttgaatatc ccgagttaaa tcttcttttt
     1021 catcgtgtaa atcgttgata tccctcaaca attgtttttt cgtttcaatt tgttggtcta
     1081 attcacttaa aactttcgct ttctttttat attcatgaat atcttgaata tacgttttat
     1141 tatcatcggt actcccacgt tcaacatcaa aaccttgctg gcacagatgt gttgttaatt
     1201 catcatggat gtgattaata gcttgtcgat tcatagctgt acgtgctgat aaccgccctt
     1261 gatgattaac aggtataaag tgaaggtgca tatgaggcgc tgattcatcc atatgcactt
     1321 tcgctgaaac gacattggct tcaccaaaac gttcactgaa gtaatcttta catgtcttaa
     1381 agtagtgttt tgtacgcgtc tttcccatac ggtcggcctc ttctttcgat aacgtaatga
     1441 tattggaata catcaccaca ctatttttct gtacacgtga cccctgttct ttatagtaat
     1501 cgactctatc tttcacacga tgatacaaat gacgttcatc ttcaactaaa tcaaagttta
     1561 agtttgtccg tttcaaatca atctcatcgt ttttgattct ccctttacgt tcttgttcca
     1621 tctctttgcc tttaacatcg ctcattttat ttttttgcgt attccaagct aatttcaaat
     1681 tgagcgccct ccccgaagct ttttgatttg tctgtggttc tctactttcg agaaccttcg
     1741 caaatatcat tttacaagcg ctgtgattgt ctaccttcga caatgcttcg cttatgtaaa
     1801 atgatggata ctctcaccgc tacgcctcaa acatgtgagg ctgattccgc aatgagagta
     1861 tagctcgata tactcttaca ttcaaatgta agagttatct tcgctctggc gaggctatca
     1921 ctacattcaa aaatatattt ttgaatgccg ttctatagct aacaaatgac atacgcatta
     1981 tcaaacaaat atgtttgaaa atatgtatgc caaatgttag ctcataccac ctcgaatcat
     2041 acatatttat tagcatacat atgtatgtta tattaatata aagataagca cacgtatgta
     2101 tgcttatcac atattaatca tgaggtggtt caatgccaaa aaaacaaaat tcaaaagaaa
     2161 aaatcttaac aattcgtgta agtcaatcgc aatacgaaaa gatttgtgca cttgcggata
     2221 ttcgagagat gaatgtgacc gaatacatac gtcatacagc cattggtaat cgcattaaac
     2281 cgacagtcat cgaacaaatt aaagaatcca gtgaagtcag tgaattgttt aaaaaagaca
     2341 acgaggcact caaatcgaat aatcaggcac tccagcaaca tattcatcag ctggaagatg
     2401 aaatcgacca actgagacaa gaaaatgacg tttttcatca tttattacaa cattttgata
     2461 gtacagcatt tttagatttc agtacatatc gtgatgatcg tccactaaaa aatgcgatta
     2521 aacgattaaa agaacagtat aaaagcaagt gatttataat tttataaatc acttgctttt
     2581 attatattaa gtagcttctg acttatggtg taataatttt aataaaagct gtgggtaatc
     2641 ggattaaacc aacggtcatt gaatatccaa aagaacaaac gacattagaa gattatgaag
     2701 aggattcaaa ggttagtgaa attattaaac ccaaatgaaa aatacaatta aaaaggaaat
     2761 gataataagg tcaaaaatga tgttctttat attccaagtt aaaaaatcag ggacactatt
     2821 aataattaac cctgctatca ataaaacctc actagcaaag tgtatgtact ttaaaaaaga
     2881 aagatcagat aaaaaagtgc ttttcataag cgaaaaaata acaatacaaa taaatgctgc
     2941 tcccataaca gcattgccta tcttataata tttttcatag tcttcagttt cttttaaaag
     3001 catttaatct ctcctaagcc gaaatccaat ttgttttaat tttaaatttt gtttgaagta
     3061 tataccaaag tcttatcttt gagtgctttt ttatcctgtt tataaaaaat ctgcaaattg
     3121 ttcacgatat ttaacatgta aaatagagcc caacacaaaa gtaattaaaa ggaaagcaag
     3181 attatataaa gatagtttcc aatgtgttat aaaaaaccaa ttttgatata gaattgctgc
     3241 tctataactt tcagctataa aatagatagg attaaattgt ataaccttta aaactaaagc
     3301 atttgtcggt aaatataaaa ttgaagatat aaaaaatatt attctcataa gagcttgcat
     3361 aagcatttgg atgtctctaa taattacagc taatgttgaa gttaatagtg ttattgtcat
     3421 tgtcagtaac agagtgtaag ggataaaaat taataattgt aatgtatata tagtagggta
     3481 atatccattt aaagcacata taagcataat taaaataagc aataacacat gggtataaaa
     3541 tttacttaag acgatataac tagataatat agataatgga aaattcattt tagctacttg
     3601 cttatatttg ctagtaactg cttttgtacc atttaaaata ccttgattca caaaaaacca
     3661 catacttatt cctacaatta accaaaaaat aaaaggtata ttctcaacag ttctattatt
     3721 tctgaaacct aaaccaaaaa taaaccaata cataaaaatc tgtattgaag gattaattaa
     3781 ttcccatgct gttcccaaat aattattttt gttagaaatt ttcatttcaa attgtgctaa
     3841 tcttttcatt aaatataaat tattaaaatg ttcttttaat ataaacaaag atgatttcat
     3901 aacattaagt tacctttcta agattatcaa tattataaat gaaacctaaa aaaaaggtaa
     3961 agcaattgtt tattgcttta ccttttctat tttaattatg gagttgtaca aaattcactg
     4021 tttccgactg ggaagcaatt atgtttgtta ccatttcctt tatgtttttt ctttttagat
     4081 ttagcaaatg cttcatgaga gttatcattc ataaaaccag ttgaagcact tgcatatgat
     4141 agtgttgtta ctaatgaaaa tggaataaga agtttagata tttttaatat tctgttcatt
     4201 tttacaaaat atacgtattc tatacaaaag cactacctat aataacgaat tatgttaact
     4261 aacatatcag ggacaaacgc tgaaatgacg gcgtttgtcc ctatttgtca tcattcctct
     4321 ttatacaact tttatacatc cctttaacga cgcgttttca tattttttca ttaagtgaac
     4381 ttttgtatct ttattcaaaa ttaaaaaacc tattaaaatc agattttgtt atactaaaaa
     4441 agcacttccc gtgcagaaag gaggtgccac atctagtgat tgtcgttctg tttgttgtga
     4501 aatatatcgt atgtccaatt attgttggac gtgttcttta tttattaaat caagaacaac
     4561 gataaaaaag aaaccccagc atatgctttg gtcggcatgc tggggttttc acatccaatg
     4621 atgtgtctgt ctgtttgtta tttttagtat atcttgcatg aagagatttg taaaattaaa
     4681 tagatattcg agtgaactaa ttatgtaatc gtcaacaata tctcaagcta gctttataac
     4741 ttagcttttt tatcattcat cgcttaaaat tatactttat atcattaaaa ggccaaaatc
     4801 ttaaaataac ttttcctacg atttgttttt tagatattaa ccccacctca gaatatctac
     4861 tatcaatact gtttgtccta ttgtctccga gaactaaata cttatcttta ggtattatca
     4921 ttttattttt agatcccttt atatttgata cattaaaatc ttctgttaaa ttttcactgg
     4981 agttattcat ttttttatta tagtcaaggt aaggttcttt gatataattt ttattgatat
     5041 ataacttgtc atttttatat tcaatttgat ctcctggttt accaatcaat cgttttataa
     5101 agtcattatt gctattttga tgaaatataa taacatctcc agattttaag tgatttaatg
     5161 atttggatat tttgctgact actactttat ctttattgtt aaacgttgga tacattgata
     5221 atcctgatac agtataaggt ttaaataaaa aattatttac taaaaataat agtaatatag
     5281 ctactattat ggctattaac cattccttaa attctttttt cattacataa tttccttttt
     5341 agattttata aattttatcc tattttaggt tatcacttat atttttaata tcctaccaaa
     5401 agtagttcat tatttataat gtcttttctt tattgaaata ataaaattta ctatcaagaa
     5461 cagaatgaac tcgttccact cgtttcaaaa aaggcaaaaa tttccagcgt taaatatttt
     5521 ttgagtgagt taaatataaa taagtgttaa agtgaaaaca tataattgtc cctaactaac
     5581 attaattttt ttaacgtaag taactcctta acgatttcaa tgccactcaa attgttgagt
     5641 ggctattttt aatgctttat ttaaattata gtagagtgca gtgttgcgat tttcatgttt
     5701 tggctaaaca accgcaataa ataaatcaag atagccttac cactaaagaa ccccaaccta
     5761 tgtcagtagg ttggggttcg gtgatatatg agttcaatat ttattagttt cagtatatac
     5821 tgtcttttta acgtatgttt tataaatatt taaattataa ataatcactt tttaaaagct
     5881 ttagttttga tatttttttc ttgtaagaat ttttctattt tagattgctc tcttccattc
     5941 actattttac tgtcattttt aatttctccg atatctttag tagatacttt ttcatgtttt
     6001 gtacttttgt taattatttg atcaagtttc ttctctaaat ttttggaata tctattagaa
     6061 tgagaaatat acttacttat tctttctgta ccttttttat aatctttagg taatttatta
     6121 tttattatat agtttttaaa attcatatca tttttttgcc ttaggctatt taagtgttta
     6181 agatttttaa tttcactttg acttaatgat tcttcagaat ctaccttatc ttcgtaatat
     6241 tcactacttt cagatagctc ttcatcttta tctaagtata gttttattga ttttttcatt
     6301 tcattttctg ttatatattg attatttgtt ttcttattaa atattctttt tggtaacggt
     6361 ttttctacta ttttctcttc attctttttt tcaatatgat tatcattgtt agaattagaa
     6421 caacttacta aaataactga aaaaatcaat aataaaaaaa tatatacttt tttcatttca
     6481 ttttcccctt tttatagatg gttatattcc aatttaaata attaactaat accttccaaa
     6541 aatatttagt aataaaataa ccaataaata ctagtataat tcctaaagta actcctttaa
     6601 taacttctat aatgtctccg gtgttaatag tactgaaacc atttataatc cccattacta
     6661 ataatattat tggtgatata atcattatcg gaactgcaat gaaataggct aatataaaag
     6721 gtgagaatag taaaagaaca aaaaaactaa atattattaa aattaagttt aaaatactaa
     6781 gctccataat agaaaaggct gcagaaaata tacttttaac acttttttta ttttctactt
     6841 tgtataatga atttacagca tttatttctt tggctatttt tacaggatca ccaagttctt
     6901 taataatttc ctcatcattt ttattatttt tatatccatt aataaaataa gactcgtatt
     6961 tagataaaat atcttctttt tcatcagtat ttatcttttt tgtttctttt tgaataatct
     7021 ttaaatattc ttctttattc attaatgcca ctctctttca caaatctatt tattgtttta
     7081 gtaaaggcat tccactcttt aagtaatgca ctgaaaaaac ttgtatatta taattagaac
     7141 gcacttcctg tgcatgaaag gaggtgtgat gtacaaatga atattgcact tttcgtcgta
     7201 acaattatca tttgtccagt tttgactggt atcattgcaa attatttgta tgataaattc
     7261 aaatgataaa aagaaaaccc ccacagccgt ccaaagcttt gtgggggttt tgatgtacaa
     7321 acaacatcgc acttttcgtt actttcagta tatctttcat gttatctttt gtaaactgtt
     7381 ctattcttcc caatcaactt gaagttgttt taaaaatgcc tctcgttctt tctctaattg
     7441 cggatcatac tcattttgtg ttgttttttc atatgtgcga tcttccaacc attttggcgt
     7501 tttttcacga ctgatatatt gtcgtgactt acctatattc gccatttgtg tctgtcgttt
     7561 actgtgaatg cgtttctcag gatcaaagat aaactccagc cattcaattt tacgtccttt
     7621 tttagctttg attttattga tttttagatt attaaatatc gtagatagtt ctcttatgat
     7681 tggagttaga acaaattgat taatattaga cattctataa gaatttggaa tatctaaacg
     7741 ttctctaaaa tcttcaaccc taattttcat ataaccagaa tgcttatatt gtttaagcat
     7801 tctaaacata tttttggcat aagtagattt aagatgtgtc atttctttta attcaaattt
     7861 agtaaagtta ttagtaatag agtttaaaat atactttaaa tctggggaag tagcaatttc
     7921 taaatatctt tcagatatat caatttcata tttataaaat agaacgaatt ttttaatttt
     7981 tgtttctgtt ctaatagtat atcgaatatc taacatttta tcgtaaactt tttgtaagtc
     8041 atctacaaat ctgtttatat tacgcgtttc tggattataa ttacttaatt cttttagctc
     8101 atcaaatgat aagtgtaacg tattagtatc ttgctctttt aatttattac acattgcaaa
     8161 aaataagttg atttcagtag ctgtaaatcg tcttaaaggc actaagttca tttcattttt
     8221 atatataaca gtttctccac tcatatgata ctcctctctt tacaaaggca taacaataaa
     8281 aaaactatgc ctttgtaaat ttttaaaaca cctcataacc cttataatta aggtaacata
     8341 tagtcaaagg cataacaata aaaaaactat gcctttaatg tggcaaaact atgcctttaa
     8401 tgtggcaaaa ctatgccttt aatgtggcaa aactatgcct ttaatg
//
