LOCUS       NZ_CP090473            11269 bp    DNA     circular CON 08-DEC-2023
DEFINITION  Klebsiella pneumoniae strain kp7450 plasmid p5, complete sequence.
ACCESSION   NZ_CP090473
VERSION     NZ_CP090473.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN24662597
            Assembly: GCF_021442045.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Cai,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2022) School of Medicine, Shanghai Jiao Tong
            University, Chongqing S. Rd., Shanghai 200025, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2020-01
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 236.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/08/2023 09:54:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,671
            CDSs (total)                      :: 5,547
            Genes (coding)                    :: 5,361
            CDSs (with protein)               :: 5,361
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 74 of 186
            Pseudo Genes (incomplete)         :: 118 of 186
            Pseudo Genes (internal stop)      :: 30 of 186
            Pseudo Genes (multiple problems)  :: 33 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP090473.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="kp7450"
                     /isolation_source="human"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p5"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="31.22337 N 121.49295 E"
                     /collection_date="2020-01"
                     /collected_by="lijianhui"
     gene            join(10387..11269,1..11)
                     /locus_tag="LZ687_RS28145"
                     /old_locus_tag="LZ687_28145"
     CDS             join(10387..11269,1..11)
                     /locus_tag="LZ687_RS28145"
                     /old_locus_tag="LZ687_28145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_044816547.1"
                     /translation="MNNAVLELFNDRLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRTGAAIDWNDRNAPAPTLTITNPDNGHAHLLYALRTSIRTAPDGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHSHWRIAVWQPELYTLDWLADFLDLNAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYDQWLQACYERATAYNLHFSSPLD
                     DNEVTGIAKSIAKWTSKNFSEDSFREFVSRTHTSEIQSIRGSKSKGGGRPSIGEPWLEH
                     GISRATYFRKLKKNEI"
                     /name="replication initiation protein"
     gene            1..1776
                     /locus_tag="LZ687_RS28150"
                     /old_locus_tag="LZ687_28150"
     CDS             1..1776
                     /locus_tag="LZ687_RS28150"
                     /old_locus_tag="LZ687_28150"
                     /inference="COORDINATES: protein motif:HMM:NF015210.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="WP_124046799.1"
                     /translation="MRFDASPFNMNSILSVEKRYEIPRNQREFSWERIQLEELWADLLR
                     NIVMDDNGTIKLHEYFIGTIVLSGVDSDDVLEVVDGQQRLSVITMMLSIISRYFRKNKN
                     DTTADNIYKKYIVTINNTLDRNSLNSDGESVIQKLTRSVGGEYFKKIVQGKEESDAKAT
                     NDEEKKIRYAVTFFKRALRKKSLCSAIFKTNDDSFTKADYDLCLNAIYKMITNYLYIVR
                     IAVDRGDDAYDIFEVLNARGINLSSIDLIKNKVFQSCTTTFPEDEARRKWVHIYEQLGS
                     VGETMTDYIRCWWLSKYGYIGEDQLYRSFKREILNENSLLTADSFLEEIYNDVSLYMSM
                     VAPSTKYWNLSHQRPILDALANINTFSVTVPRPFILSLLRTRRDKSRLMKQSELIETLE
                     FLEKFHFKFNAVCRMRPSGIDSKYSVFAVRADRAVSKASLKNTIDETVDFLKRKAPSEK
                     VFKDCLMKNIEYKDDKLSQRKLIIYIFETLERLAVGTKELKYHMVSIEHIGSQSNFSPT
                     YVGKLGNLLPLCFDINRDCENMPLAGKLPEYARSRLELVKQFNTANAAKNTWEVSDAQT
                     RQEQITNALNAAFTL"
                     /name="DUF262 domain-containing protein"
     gene            2008..2334
                     /locus_tag="LZ687_RS28155"
                     /old_locus_tag="LZ687_28155"
     CDS             2008..2334
                     /locus_tag="LZ687_RS28155"
                     /old_locus_tag="LZ687_28155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_038885858.1"
                     /translation="MEKRTKEIKIRLTETEHQRLLDRCDRTHLAEWLRQLGLGEHTSRK
                     RRVPEVAPELLRQVSGIGNNLNQIARRLNQADSLTPSERASLLVVLTSLDRQLGDLLEQ
                     NRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2543..>2965
                     /locus_tag="LZ687_RS28160"
                     /old_locus_tag="LZ687_28160"
                     /pseudo=""
     CDS             2543..>2965
                     /locus_tag="LZ687_RS28160"
                     /old_locus_tag="LZ687_28160"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3012..3497
                     /locus_tag="LZ687_RS28165"
                     /old_locus_tag="LZ687_28165"
     CDS             3012..3497
                     /locus_tag="LZ687_RS28165"
                     /old_locus_tag="LZ687_28165"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_139914218.1"
                     /translation="MSEILNLAKAFEEKSKQQAQDTEQSVKREFARLETSISAELQSSS
                     QSISNAIREQNGRLTGMLRASVSTAMAWIFLCFALLIGIMGGIIWFQGTIITSRLSEMD
                     SYSQQLESLKAKSGAGVAIYSDDKHRNTYMVVLPKQAHGVETYTSTTDNTVVKYTAK"
                     /name="MbeB family mobilization protein"
     gene            3567..3791
                     /locus_tag="LZ687_RS28170"
                     /old_locus_tag="LZ687_28170"
     CDS             3567..3791
                     /locus_tag="LZ687_RS28170"
                     /old_locus_tag="LZ687_28170"
                     /inference="COORDINATES: protein motif:HMM:NF016769.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_223825645.1"
                     /translation="MEKQLLSALESLQTSYEQQQQAWQDSYSSLQHMFEATSQALTHSD
                     RVCQHLSSQVDSLRVQVESLSRNVSRLMR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3947..4279
                     /locus_tag="LZ687_RS28175"
                     /old_locus_tag="LZ687_28175"
     CDS             3947..4279
                     /locus_tag="LZ687_RS28175"
                     /old_locus_tag="LZ687_28175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_044816545.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSLTDAEKAVLKSLTQ
                     QLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4281..4565
                     /gene="nadS"
                     /locus_tag="LZ687_RS28180"
                     /old_locus_tag="LZ687_28180"
     CDS             4281..4565
                     /gene="nadS"
                     /locus_tag="LZ687_RS28180"
                     /old_locus_tag="LZ687_28180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_001697687.1"
                     /translation="MSFFDELKASLEEAVEIKNGVREPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="nadS"
     gene            4758..5651
                     /locus_tag="LZ687_RS28185"
                     /old_locus_tag="LZ687_28185"
     CDS             4758..5651
                     /locus_tag="LZ687_RS28185"
                     /old_locus_tag="LZ687_28185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_044816547.1"
                     /translation="MNNAVLELFNDRLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRTGAAIDWNDRNAPAPTLTITNPDNGHAHLLYALRTSIRTAPDGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHSHWRIAVWQPELYTLDWLADFLDLNAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYDQWLQACYERATAYNLHFSSPLD
                     DNEVTGIAKSIAKWTSKNFSEDSFREFVSRTHTSEIQSIRGSKSKGGGRPSIGEPWLEH
                     GISRATYFRKLKKNEI"
                     /name="replication initiation protein"
     gene            5641..7416
                     /locus_tag="LZ687_RS28190"
                     /old_locus_tag="LZ687_28190"
     CDS             5641..7416
                     /locus_tag="LZ687_RS28190"
                     /old_locus_tag="LZ687_28190"
                     /inference="COORDINATES: protein motif:HMM:NF015210.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="WP_124046799.1"
                     /translation="MRFDASPFNMNSILSVEKRYEIPRNQREFSWERIQLEELWADLLR
                     NIVMDDNGTIKLHEYFIGTIVLSGVDSDDVLEVVDGQQRLSVITMMLSIISRYFRKNKN
                     DTTADNIYKKYIVTINNTLDRNSLNSDGESVIQKLTRSVGGEYFKKIVQGKEESDAKAT
                     NDEEKKIRYAVTFFKRALRKKSLCSAIFKTNDDSFTKADYDLCLNAIYKMITNYLYIVR
                     IAVDRGDDAYDIFEVLNARGINLSSIDLIKNKVFQSCTTTFPEDEARRKWVHIYEQLGS
                     VGETMTDYIRCWWLSKYGYIGEDQLYRSFKREILNENSLLTADSFLEEIYNDVSLYMSM
                     VAPSTKYWNLSHQRPILDALANINTFSVTVPRPFILSLLRTRRDKSRLMKQSELIETLE
                     FLEKFHFKFNAVCRMRPSGIDSKYSVFAVRADRAVSKASLKNTIDETVDFLKRKAPSEK
                     VFKDCLMKNIEYKDDKLSQRKLIIYIFETLERLAVGTKELKYHMVSIEHIGSQSNFSPT
                     YVGKLGNLLPLCFDINRDCENMPLAGKLPEYARSRLELVKQFNTANAAKNTWEVSDAQT
                     RQEQITNALNAAFTL"
                     /name="DUF262 domain-containing protein"
     gene            7636..7962
                     /locus_tag="LZ687_RS28195"
                     /old_locus_tag="LZ687_28195"
     CDS             7636..7962
                     /locus_tag="LZ687_RS28195"
                     /old_locus_tag="LZ687_28195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_038885858.1"
                     /translation="MEKRTKEIKIRLTETEHQRLLDRCDRTHLAEWLRQLGLGEHTSRK
                     RRVPEVAPELLRQVSGIGNNLNQIARRLNQADSLTPSERASLLVVLTSLDRQLGDLLEQ
                     NRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            8171..>8593
                     /locus_tag="LZ687_RS28200"
                     /old_locus_tag="LZ687_28200"
                     /pseudo=""
     CDS             8171..>8593
                     /locus_tag="LZ687_RS28200"
                     /old_locus_tag="LZ687_28200"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8640..9125
                     /locus_tag="LZ687_RS28205"
                     /old_locus_tag="LZ687_28205"
     CDS             8640..9125
                     /locus_tag="LZ687_RS28205"
                     /old_locus_tag="LZ687_28205"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_139914218.1"
                     /translation="MSEILNLAKAFEEKSKQQAQDTEQSVKREFARLETSISAELQSSS
                     QSISNAIREQNGRLTGMLRASVSTAMAWIFLCFALLIGIMGGIIWFQGTIITSRLSEMD
                     SYSQQLESLKAKSGAGVAIYSDDKHRNTYMVVLPKQAHGVETYTSTTDNTVVKYTAK"
                     /name="MbeB family mobilization protein"
     gene            9211..9420
                     /locus_tag="LZ687_RS28210"
                     /old_locus_tag="LZ687_28210"
     CDS             9211..9420
                     /locus_tag="LZ687_RS28210"
                     /old_locus_tag="LZ687_28210"
                     /inference="COORDINATES: protein motif:HMM:NF016769.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_234542842.1"
                     /translation="MSALESLQTSYEQQQQAWQDSYSSLQHMFEATSQALTHSDRVCQH
                     LSSQVDSLRVQVESLSRNVSRLMR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            9336..9515
                     /locus_tag="LZ687_RS28215"
                     /old_locus_tag="LZ687_28215"
     CDS             9336..9515
                     /locus_tag="LZ687_RS28215"
                     /old_locus_tag="LZ687_28215"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_169540246.1"
                     /translation="MSALEQSGGQLARAGRELKQERQPLDALIAQHDRARAEKLREQQP
                     EPQKKPTRYYGPSM"
                     /name="hypothetical protein"
     gene            9576..9908
                     /locus_tag="LZ687_RS28220"
                     /old_locus_tag="LZ687_28220"
     CDS             9576..9908
                     /locus_tag="LZ687_RS28220"
                     /old_locus_tag="LZ687_28220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_044816545.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSLTDAEKAVLKSLTQ
                     QLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            9910..10194
                     /gene="nadS"
                     /locus_tag="LZ687_RS28225"
                     /old_locus_tag="LZ687_28225"
     CDS             9910..10194
                     /gene="nadS"
                     /locus_tag="LZ687_RS28225"
                     /old_locus_tag="LZ687_28225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_001697687.1"
                     /translation="MSFFDELKASLEEAVEIKNGVREPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="nadS"
     Typing          2325..3883
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.743"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7953..9208
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.613"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagatttg atgcgtcacc atttaatatg aactccattc tttctgtaga aaaaagatat
       61 gagatcccaa gaaatcagcg agagtttagc tgggagcgaa ttcagcttga ggagctttgg
      121 gctgatcttc taagaaatat agtgatggat gataacggca ctataaaatt acacgagtac
      181 tttattggaa ccattgtgct atcaggtgtt gatagtgatg atgttttaga agttgtggat
      241 ggacagcaac gattgtctgt aataaccatg atgctttcta taatatcaag gtattttaga
      301 aagaacaaaa atgacacaac tgcggacaat atatataaaa aatatatcgt aacaataaat
      361 aacactctgg atagaaatag tttaaattct gatggtgagt ctgttattca aaagctaaca
      421 aggagcgttg gaggtgagta ttttaaaaaa atagttcaag gcaaggaaga gtctgacgcc
      481 aaagcaacga atgatgaaga aaagaaaata agatatgctg ttacgttctt taaaagggct
      541 cttcgcaaaa aatcattatg tagcgccata tttaaaacaa atgatgatag ttttacgaag
      601 gctgattatg acttatgtct taatgctatc tataaaatga taaccaacta tctgtacata
      661 gtcaggatag cggttgatcg aggcgacgat gcatatgaca tatttgaagt tctaaatgcc
      721 agaggtatta acctcagttc aatagacctt ataaagaata aggttttcca gtcttgcact
      781 acaactttcc cagaagatga agccagaagg aaatgggttc acatatacga acagctaggt
      841 tcagttggcg agaccatgac cgattacatc cgttgttggt ggctatctaa atatggatat
      901 attggtgaag atcagctcta tcgatcattc aaaagagaaa ttttaaatga aaatagcttg
      961 ttaacagctg attcattttt agaagaaata tataatgatg tatctcttta tatgagtatg
     1021 gtagctccat ctacaaaata ctggaacctg tctcaccaga gacctatatt agacgcctta
     1081 gcaaatatca acacatttag cgttactgtt ccaagacctt tcattttatc tcttctcaga
     1141 acaagaagag ataaatcaag gcttatgaaa cagtctgagc ttattgagac gctagagttc
     1201 cttgagaagt tccattttaa atttaatgct gtatgtcgaa tgagaccttc aggtattgac
     1261 tcaaaatatt cagtttttgc agtcagagct gatagagctg tgagcaaggc ttcattgaaa
     1321 aacacaatcg atgagacagt tgatttcctg aaaagaaagg caccaagcga aaaggtgttt
     1381 aaagactgct taatgaaaaa tatcgagtat aaggacgata aactatctca aagaaaactc
     1441 attatctaca tatttgaaac actagagcga ctagcagtag gaactaaaga gcttaaatat
     1501 catatggttt cgattgaaca tataggctct cagtcgaatt tcagtccaac ttatgttggt
     1561 aagctgggta accttctgcc gctttgtttc gatataaata gagactgtga aaatatgcca
     1621 ttagctggga aattgccaga atatgctcga tcacgattag agttagtgaa gcaatttaac
     1681 actgctaacg cagctaaaaa tacttgggaa gtaagtgatg cccaaacgag gcaagaacag
     1741 ataacaaacg ccctcaacgc tgccttcacg ctctgatgtg agactaaaca acctatatca
     1801 gataacagcc ctctttagca gagggctgtt tttttgcgga tagcatagaa tctgacatac
     1861 tacttcatac tataagccag tacacacagc gtcccctctt acgaggtgtc gccgtgactg
     1921 gttcaggggg ctcgccgccc cccgaaaccc ccagcatcca ctgcgaaaaa ttctcacggt
     1981 ggtgcgaatt ttttctcagc ggagcctatg gaaaagcgaa cgaaagagat caaaatccgg
     2041 ctcaccgaaa ccgagcatca gcgactgctt gatcgctgcg accgcactca ccttgcggag
     2101 tggctgcgtc agcttggtct gggtgaacac acctcccgca agcgccgtgt gcctgaggtg
     2161 gcgcccgaac tgctgcggca ggtcagcggg attggcaata acttgaacca gatcgcccgt
     2221 cgcctgaacc aggctgattc cctgacgcct tctgaacgtg cctcgctgct cgtcgtgctg
     2281 accagccttg accggcagct tggcgacctg ctggagcaga accgtgatcg ttaaaattca
     2341 ctcacggggt gcgggttcgg gcagcgggcc ggtggattac ctgctgggta aagaccggca
     2401 gcgtgaacag gccagcgttc tgcgcggcaa ccctgaacat gtgcgcgagc tgatcgacgg
     2461 ctgcgacttt gcccgcgctt atacttccgg cgtgctgtca tttcaggagc cggacattgc
     2521 agacgctgag aaatcgcgcc tgatggacga atgggagcac acgctgctga ccggccttga
     2581 tcgcgaccag tacgcctgcc tgtgggtgga gcaccgtgac aagggacggc ttgagctgaa
     2641 ttttgtcatc ccgaacatcg aattgcagag tggaaaacgc ctgcaaccct actttgaccg
     2701 cgccgaccgt ccccgcgtca acgcctggca gaccctgacc aacgaccggc tggggctgcg
     2761 cgacccgaat gacccgacct accgccgtcc gctgacgcag gccagcgatt tgccccgcga
     2821 caaacagcag gcggcagaga aaatcaccgc cggcctgatg aacctgatgc agcagggggt
     2881 gatccgcagc cggcaggacg tggtgacgca gctggaaagc tacggcctga ccgtagcgcg
     2941 ggaaacgaaa agcagcatca gcattgccga tccggacggc ggccgaaata tccggctgaa
     3001 aggaatgatt tatgagcgag attttaaatt tggcgaaggc cttcgaggag aaatcgaagc
     3061 agcaggcgca ggataccgag cagagcgtga agcgcgagtt cgcgaggctg gagacgtcta
     3121 tcagcgcgga actgcaatca agctcgcaga gcatcagcaa cgctatccgc gagcagaacg
     3181 gcagactgac gggcatgctc agagcctctg tctcaacggc gatggcgtgg atattcctct
     3241 gcttcgccct gctgatcggc atcatgggcg ggataatctg gtttcagggc acgatcatca
     3301 cctcgcgcct gagcgagatg gacagctaca gccaacagct ggagagcctg aaagcgaaaa
     3361 gcggcgcggg cgtggcgatc tacagcgacg acaagcaccg aaacacctac atggtggtgc
     3421 tgccgaagca ggcgcacggc gtggaaacct atacatcgac gaccgacaac accgtagtga
     3481 aatacacggc gaaataaccc cgtcagagcg ccctgaaacg acacagcgcg tttttaacac
     3541 cgaggatgac caaaaccatg accgagatgg aaaaacagct tctgagcgcc ttagagagct
     3601 tacagacaag ctacgagcaa cagcagcagg cgtggcagga cagctacagc agcttgcagc
     3661 acatgttcga ggctacctca caggcgctga cgcacagcga cagggtgtgt cagcacttga
     3721 gcagtcaggt ggacagcttg cgcgtgcagg tcgagagctt aagcaggaac gtcagccgct
     3781 tgatgcgctg attgcacagc acgaccgcgc ccgtgcggag aagctgcgcg agcagcagcc
     3841 ggagccgcag aaaaagccga cccgctatta cggcccctcg atgtgaccgc ttttctgaaa
     3901 ttaaaattaa gtgtactatg tacgccattg aacacaggag acagccgtga gtcacgcgat
     3961 tgagtttatc gagaccccga tgtttacccg tcagataaaa cagatcgcca cggatgaaga
     4021 actcagggag ctgcaaaaga cgctgattga gtcaccggat aaaggcgatc tcatccagaa
     4081 aaccggtggg ctgcgcaaaa tcagaatggc gacgggcaac cagggaaaaa gcggcagtgc
     4141 gagggttatt tactttctgg ccaccgccga ggtgatttat ctggtgatgg cctacccgaa
     4201 aagcaccaaa gacagcctga cggacgcgga gaaagccgtg ctgaagtcgc tgacccaaca
     4261 actgaaagac gaggtgtgac atgagttttt ttgacgagct gaaagcatcg ctggaagaag
     4321 ccgttgaaat caaaaacggc gtcagagagc ctgcccgcgt cacccgttac gagctggcag
     4381 acgtgaaagc catcagggca cagctgaacg tatctcagag cgagatggcg aaagtgctgg
     4441 ggaccagcct cgacactatc aagagctggg aaaccggacg ccgtaatccg accggactgg
     4501 cggcgaaggt gctggcaacc atccaggcga acccgaaatt cttccaggag ctggcggcgc
     4561 actaagccgt tgtgacagaa ggattcgaca ggcgaggccg ttcgcgtgta atttaacggc
     4621 aacagaaaaa acaaagcccc ggaaatcatg ctctactttg gcgagcggac atgataacca
     4681 gggccaggtc gaaaccttga aaggatatta gcacatgaac cgtgccagac aacagcccgc
     4741 cataagtgca gggcgctttg aataacgcag tactcgaact tttcaacgac cgcttaccgc
     4801 acaagccgta cttctcggat gatttacact ttggtgtacg cattgccggt aaggaacgcg
     4861 ccattctggc gaaatacatc cagttcaacc agccgcacgc catgttctgg ctgggattcg
     4921 acgtcgatcg caccggtgcg gccatcgact ggaacgaccg gaacgcgcct gcgcccacgc
     4981 tgaccatcac caaccccgac aacggacacg cgcatctgct gtacgccctc agaacctcga
     5041 tacgtaccgc gccggacgga aaaatgaagc cgctgcgcta cgctgccgct gtggagaacg
     5101 cgctacgtaa aaagttagag gctgacgcgg ggtattcggg gctaatctgc aaaaatccga
     5161 accatagcca ctggagaatt gccgtatggc agccggaact ctacacgctg gactggctgg
     5221 ctgactttct cgacctgaac gcggcgaacg acaaagaaat cgttgccgac tatggtttag
     5281 gccgtaactg cacgctgttc gacaaaaccc gcaagtgggc ataccgcgct atccgtcagg
     5341 gctggcctga gtatgaccag tggctgcaag cctgttatga acgtgctaca gcctataacc
     5401 ttcatttttc ttcgcctctt gacgataacg aagtaactgg aattgccaaa agcatagcta
     5461 aatggacaag taaaaatttt tccgaagatt cattcaggga gtttgtttct cggacacata
     5521 cctctgagat ccagtcaata cggggtagta aaagtaaggg aggcggaaga ccttctatag
     5581 gggagccgtg gttagaacat ggcatcagca gagctacata cttcaggaaa ctaaaaaaaa
     5641 atgagatttg atgcgtcacc atttaatatg aactccattc tttctgtaga aaaaagatat
     5701 gagatcccaa gaaatcagcg agagtttagc tgggagcgaa ttcagcttga ggagctttgg
     5761 gctgatcttc taagaaatat agtgatggat gataacggca ctataaaatt acacgagtac
     5821 tttattggaa ccattgtgct atcaggtgtt gatagtgatg atgttttaga agttgtggat
     5881 ggacagcaac gattgtctgt aataaccatg atgctttcta taatatcaag gtattttaga
     5941 aagaacaaaa atgacacaac tgcggacaat atatataaaa aatatatcgt aacaataaat
     6001 aacactctgg atagaaatag tttaaattct gatggtgagt ctgttattca aaagctaaca
     6061 aggagcgttg gaggtgagta ttttaaaaaa atagttcaag gcaaggaaga gtctgacgcc
     6121 aaagcaacga atgatgaaga aaagaaaata agatatgctg ttacgttctt taaaagggct
     6181 cttcgcaaaa aatcattatg tagcgccata tttaaaacaa atgatgatag ttttacgaag
     6241 gctgattatg acttatgtct taatgctatc tataaaatga taaccaacta tctgtacata
     6301 gtcaggatag cggttgatcg aggcgacgat gcatatgaca tatttgaagt tctaaatgcc
     6361 agaggtatta acctcagttc aatagacctt ataaagaata aggttttcca gtcttgcact
     6421 acaactttcc cagaagatga agccagaagg aaatgggttc acatatacga acagctaggt
     6481 tcagttggcg agaccatgac cgattacatc cgttgttggt ggctatctaa atatggatat
     6541 attggtgaag atcagctcta tcgatcattc aaaagagaaa ttttaaatga aaatagcttg
     6601 ttaacagctg attcattttt agaagaaata tataatgatg tatctcttta tatgagtatg
     6661 gtagctccat ctacaaaata ctggaacctg tctcaccaga gacctatatt agacgcctta
     6721 gcaaatatca acacatttag cgttactgtt ccaagacctt tcattttatc tcttctcaga
     6781 acaagaagag ataaatcaag gcttatgaaa cagtctgagc ttattgagac gctagagttc
     6841 cttgagaagt tccattttaa atttaatgct gtatgtcgaa tgagaccttc aggtattgac
     6901 tcaaaatatt cagtttttgc agtcagagct gatagagctg tgagcaaggc ttcattgaaa
     6961 aacacaatcg atgagacagt tgatttcctg aaaagaaagg caccaagcga aaaggtgttt
     7021 aaagactgct taatgaaaaa tatcgagtat aaggacgata aactatctca aagaaaactc
     7081 attatctaca tatttgaaac actagagcga ctagcagtag gaactaaaga gcttaaatat
     7141 catatggttt cgattgaaca tataggctct cagtcgaatt tcagtccaac ttatgttggt
     7201 aagctgggta accttctgcc gctttgtttc gatataaata gagactgtga aaatatgcca
     7261 ttagctggga aattgccaga atatgctcga tcacgattag agttagtgaa gcaatttaac
     7321 actgctaacg cagctaaaaa tacttgggaa gtaagtgatg cccaaacgag gcaagaacag
     7381 ataacaaacg ccctcaacgc tgccttcacg ctctgatgtg agactaaaca acctatatca
     7441 gataacagcc ctctttagca gagggctgtt tttttgcgga tagcatagaa tctgacatac
     7501 tacttcaagt acacacagcg tccctcttac gaggtgtcgc cgtgactggt tcagggggct
     7561 cgccgccccc cgaaaccccc agcatccact gcgaaaaatt ctcacggtgg tgcgaatttt
     7621 ttctcagcgg agcctatgga aaagcgaacg aaagagatca aaatccggct caccgaaacc
     7681 gagcatcagc gactgcttga tcgctgcgac cgcactcacc ttgcggagtg gctgcgtcag
     7741 cttggtctgg gtgaacacac ctcccgcaag cgccgtgtgc ctgaggtggc gcccgaactg
     7801 ctgcggcagg tcagcgggat tggcaataac ttgaaccaga tcgcccgtcg cctgaaccag
     7861 gctgattccc tgacgccttc tgaacgtgcc tcgctgctcg tcgtgctgac cagccttgac
     7921 cggcagcttg gcgacctgct ggagcagaac cgtgatcgtt aaaattcact cacggggtgc
     7981 gggttcgggc agcgggccgg tggattacct gctgggtaaa gaccggcagc gtgaacaggc
     8041 cagcgttctg cgcggcaacc ctgaacatgt gcgcgagctg atcgacggct gcgactttgc
     8101 ccgcgcttat acttccggcg tgctgtcatt tcaggagccg gacattgcag acgctgagaa
     8161 atcgcgcctg atggacgaat gggagcacac gctgctgacc ggccttgatc gcgaccagta
     8221 cgcctgcctg tgggtggagc accgtgacaa gggacggctt gagctgaatt ttgtcatccc
     8281 gaacatcgaa ttgcagagtg gaaaacgcct gcaaccctac tttgaccgcg ccgaccgtcc
     8341 ccgcgtcaac gcctggcaga ccctgaccaa cgaccggctg gggctgcgcg acccgaatga
     8401 cccgacctac cgccgtccgc tgacgcaggc cagcgatttg ccccgcgaca aacagcaggc
     8461 ggcagagaaa atcaccgccg gcctgatgaa cctgatgcag cagggggtga tccgcagccg
     8521 gcaggacgtg gtgacgcagc tggaaagcta cggcctgacc gtagcgcggg aaacgaaaag
     8581 cagcatcagc attgccgatc cggacggcgg ccgaaatatc cggctgaaag gaatgattta
     8641 tgagcgagat tttaaatttg gcgaaggcct tcgaggagaa atcgaagcag caggcgcagg
     8701 ataccgagca gagcgtgaag cgcgagttcg cgaggctgga gacgtctatc agcgcggaac
     8761 tgcaatcaag ctcgcagagc atcagcaacg ctatccgcga gcagaacggc agactgacgg
     8821 gcatgctcag agcctctgtc tcaacggcga tggcgtggat attcctctgc ttcgccctgc
     8881 tgatcggcat catgggcggg ataatctggt ttcagggcac gatcatcacc tcgcgcctga
     8941 gcgagatgga cagctacagc caacagctgg agagcctgaa agcgaaaagc ggcgcgggcg
     9001 tggcgatcta cagcgacgac aagcaccgaa acacctacat ggtggtgctg ccgaagcagg
     9061 cgcacggcgt ggaaacctat acatcgacga ccgacaacac cgtagtgaaa tacacggcga
     9121 aataaccccg tcagagcgcc ctgaaacgac acagcgcgtt tttaacaccg aggatgacca
     9181 aaaccatgac cgagatggaa aaaacagctt ctgagcgcct tagagagctt acagacaagc
     9241 tacgagcaac agcagcaggc gtggcaggac agctacagca gcttgcagca catgttcgag
     9301 gctacctcac aggcgctgac gcacagcgac agggtgtgtc agcacttgag cagtcaggtg
     9361 gacagcttgc gcgtgcaggt cgagagctta agcaggaacg tcagccgctt gatgcgctga
     9421 ttgcacagca cgaccgcgcc cgtgcggaga agctgcgcga gcagcagccg gagccgcaga
     9481 aaaagccgac ccgctattac ggcccctcga tgtgaccgct tttctgaaat taaaattaag
     9541 tgtactatgt acgccattga acacaggaga cagccgtgag tcacgcgatt gagtttatcg
     9601 agaccccgat gtttacccgt cagataaaac agatcgccac ggatgaagaa ctcagggagc
     9661 tgcaaaagac gctgattgag tcaccggata aaggcgatct catccagaaa accggtgggc
     9721 tgcgcaaaat cagaatggcg acgggcaacc agggaaaaag cggcagtgcg agggttattt
     9781 actttctggc caccgccgag gtgatttatc tggtgatggc ctacccgaaa agcaccaaag
     9841 acagcctgac ggacgcggag aaagccgtgc tgaagtcgct gacccaacaa ctgaaagacg
     9901 aggtgtgaca tgagtttttt tgacgagctg aaagcatcgc tggaagaagc cgttgaaatc
     9961 aaaaacggcg tcagagagcc tgcccgcgtc acccgttacg agctggcaga cgtgaaagcc
    10021 atcagggcac agctgaacgt atctcagagc gagatggcga aagtgctggg gaccagcctc
    10081 gacactatca agagctggga aaccggacgc cgtaatccga ccggactggc ggcgaaggtg
    10141 ctggcaacca tccaggcgaa cccgaaattc ttccaggagc tggcggcgca ctaagccgtt
    10201 gtgacagaag gattcgacag gcgaggccgt tcgcgtgtaa tttaacggca acagaaaaaa
    10261 caaagccccg gaaatcatgc tctactttgg cgagcggaca tgataaccag ggccaggtcg
    10321 aaaccttgaa aggatattag cacatgaacc gtgccagaca acagcccgcc ataagtgcag
    10381 ggcgctttga ataacgcagt actcgaactt ttcaacgacc gcttaccgca caagccgtac
    10441 ttctcggatg atttacactt tggtgtacgc attgccggta aggaacgcgc cattctggcg
    10501 aaatacatcc agttcaacca gccgcacgcc atgttctggc tgggattcga cgtcgatcgc
    10561 accggtgcgg ccatcgactg gaacgaccgg aacgcgcctg cgcccacgct gaccatcacc
    10621 aaccccgaca acggacacgc gcatctgctg tacgccctca gaacctcgat acgtaccgcg
    10681 ccggacggaa aaatgaagcc gctgcgctac gctgccgctg tggagaacgc gctacgtaaa
    10741 aagttagagg ctgacgcggg gtattcgggg ctaatctgca aaaatccgaa ccatagccac
    10801 tggagaattg ccgtatggca gccggaactc tacacgctgg actggctggc tgactttctc
    10861 gacctgaacg cggcgaacga caaagaaatc gttgccgact atggtttagg ccgtaactgc
    10921 acgctgttcg acaaaacccg caagtgggca taccgcgcta tccgtcaggg ctggcctgag
    10981 tatgaccagt ggctgcaagc ctgttatgaa cgtgctacag cctataacct tcatttttct
    11041 tcgcctcttg acgataacga agtaactgga attgccaaaa gcatagctaa atggacaagt
    11101 aaaaattttt ccgaagattc attcagggag tttgtttctc ggacacatac ctctgagatc
    11161 cagtcaatac ggggtagtaa aagtaaggga ggcggaagac cttctatagg ggagccgtgg
    11221 ttagaacatg gcatcagcag agctacatac ttcaggaaac taaaaaaaa
//
