LOCUS       NZ_CP090472            12274 bp    DNA     circular CON 08-DEC-2023
DEFINITION  Klebsiella pneumoniae strain kp7450 plasmid p4, complete sequence.
ACCESSION   NZ_CP090472
VERSION     NZ_CP090472.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN24662597
            Assembly: GCF_021442045.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12274)
  AUTHORS   Cai,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2022) School of Medicine, Shanghai Jiao Tong
            University, Chongqing S. Rd., Shanghai 200025, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2020-01
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 236.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/08/2023 09:54:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,671
            CDSs (total)                      :: 5,547
            Genes (coding)                    :: 5,361
            CDSs (with protein)               :: 5,361
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 74 of 186
            Pseudo Genes (incomplete)         :: 118 of 186
            Pseudo Genes (internal stop)      :: 30 of 186
            Pseudo Genes (multiple problems)  :: 33 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP090472.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12274
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="kp7450"
                     /isolation_source="human"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="31.22337 N 121.49295 E"
                     /collection_date="2020-01"
                     /collected_by="lijianhui"
     gene            1..798
                     /locus_tag="LZ687_RS28075"
                     /old_locus_tag="LZ687_28075"
     CDS             1..798
                     /locus_tag="LZ687_RS28075"
                     /old_locus_tag="LZ687_28075"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="WP_043907054.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            complement(1205..>1357)
                     /locus_tag="LZ687_RS28080"
                     /old_locus_tag="LZ687_28080"
                     /pseudo=""
     CDS             complement(1205..>1357)
                     /locus_tag="LZ687_RS28080"
                     /old_locus_tag="LZ687_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            1733..2680
                     /locus_tag="LZ687_RS28085"
                     /old_locus_tag="LZ687_28085"
     CDS             1733..2680
                     /locus_tag="LZ687_RS28085"
                     /old_locus_tag="LZ687_28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     gene            3088..3480
                     /locus_tag="LZ687_RS28090"
                     /old_locus_tag="LZ687_28090"
     CDS             3088..3480
                     /locus_tag="LZ687_RS28090"
                     /old_locus_tag="LZ687_28090"
                     /inference="COORDINATES: protein motif:HMM:NF037437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            3462..3800
                     /locus_tag="LZ687_RS28095"
                     /old_locus_tag="LZ687_28095"
     CDS             3462..3800
                     /locus_tag="LZ687_RS28095"
                     /old_locus_tag="LZ687_28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3781..5235
                     /locus_tag="LZ687_RS28100"
                     /old_locus_tag="LZ687_28100"
     CDS             3781..5235
                     /locus_tag="LZ687_RS28100"
                     /old_locus_tag="LZ687_28100"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5517..5798)
                     /locus_tag="LZ687_RS28105"
                     /old_locus_tag="LZ687_28105"
     CDS             complement(5517..5798)
                     /locus_tag="LZ687_RS28105"
                     /old_locus_tag="LZ687_28105"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            6142..6931
                     /gene="blaOXA"
                     /locus_tag="LZ687_RS28110"
                     /old_locus_tag="LZ687_28110"
                     /pseudo=""
     CDS             6142..6931
                     /gene="blaOXA"
                     /locus_tag="LZ687_RS28110"
                     /old_locus_tag="LZ687_28110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /GO_function="GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family class D beta-lactamase"
                     /name="blaOXA"
     gene            complement(7338..>7490)
                     /locus_tag="LZ687_RS28115"
                     /old_locus_tag="LZ687_28115"
                     /pseudo=""
     CDS             complement(7338..>7490)
                     /locus_tag="LZ687_RS28115"
                     /old_locus_tag="LZ687_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            7866..8813
                     /locus_tag="LZ687_RS28120"
                     /old_locus_tag="LZ687_28120"
     CDS             7866..8813
                     /locus_tag="LZ687_RS28120"
                     /old_locus_tag="LZ687_28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     gene            9221..9613
                     /locus_tag="LZ687_RS28125"
                     /old_locus_tag="LZ687_28125"
     CDS             9221..9613
                     /locus_tag="LZ687_RS28125"
                     /old_locus_tag="LZ687_28125"
                     /inference="COORDINATES: protein motif:HMM:NF037437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            9595..9933
                     /locus_tag="LZ687_RS28130"
                     /old_locus_tag="LZ687_28130"
     CDS             9595..9933
                     /locus_tag="LZ687_RS28130"
                     /old_locus_tag="LZ687_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            9914..11368
                     /locus_tag="LZ687_RS28135"
                     /old_locus_tag="LZ687_28135"
     CDS             9914..11368
                     /locus_tag="LZ687_RS28135"
                     /old_locus_tag="LZ687_28135"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(11650..11931)
                     /locus_tag="LZ687_RS28140"
                     /old_locus_tag="LZ687_28140"
     CDS             complement(11650..11931)
                     /locus_tag="LZ687_RS28140"
                     /old_locus_tag="LZ687_28140"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     ARG             2..795
                     /name="blaOXA-232"
                     /gene="blaOXA-232"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             6717..6931
                     /name="OXA-232"
                     /gene="OXA-232"
                     /gene_name="OXA beta-lactamase; OXA-48-like beta-lactamase"
                     /tool="rgi"
                     /drug_class="carbapenem; penam"
                     /identity="100.0"
                     /coverage="26.79"
     ARG             6143..6687
                     /name="OXA-232"
                     /gene="OXA-232"
                     /gene_name="OXA beta-lactamase; OXA-48-like beta-lactamase"
                     /tool="rgi"
                     /drug_class="carbapenem; penam"
                     /identity="99.32"
                     /coverage="68.3"
     Typing          1746..2667
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          7879..8800
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          3782..5232
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9915..11365
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcgtgtat tagccttatc ggctgtgttt ttggtggcat cgattatcgg aatgccagcg
       61 gtagcaaagg aatggcaaga aaacaaaagt tggaatgctc actttactga acataaatca
      121 cagggcgtag ttgtgctctg gaatgagaat aagcagcaag gatttaccaa taatcttaaa
      181 cgggcgaacc aagcattttt acccgcatct acctttaaaa ttcccaatag cttgatcgcc
      241 ctcgatttgg gcgtggttaa ggatgaacac caagtcttta agtgggatgg acagacgcgt
      301 gatatcgccg cttggaatcg tgaccatgac ttaattaccg cgatgaagta ctcagttgtg
      361 cctgtttatc aagaatttgc ccgccaaatt ggtgaggcac gtatgagtaa aatgctgcac
      421 gccttcgatt atggcaatga ggatatctcg ggcaatgtag acagtttttg gctcgatggt
      481 ggtattcgca tttcggctac ccagcaaatc gcttttttac gcaagctgta tcacaacaag
      541 ctgcacgttt ctgagcgtag tcagcgcatc gtgaaacaag ccatgctgac cgaagccaat
      601 ggcgactata ttattcgggc taaaacggga tactcgacta gtatcgaacc taagattggc
      661 tggtgggttg gttgggttga acttgatgat aatgtgtggt tttttgcgat gaatatggat
      721 atgcccacat cggatggttt agggctgcgc caagccatca caaaagaagt gctcaaacag
      781 gagaaaatta ttccctagaa gtggttagcg cgtatttgtg tgaaatagcc gtcatataag
      841 ctgtaaagat atatggacaa aatgctgatg ctcgatgcgc ttgtagttaa tggctcatac
      901 ttaatcggtc aggcctaatc gacttaagct ccgcgcgtta actttcagcc tgagcattag
      961 catcacattc ctaaacgtaa aaagcctagc caatttgcta ggcttttttg ttatctcaat
     1021 ggccatggtc actcaacatg ggggtgtgaa taaaggagtg gcgatgtcag taaaggtgaa
     1081 tgccgctgcg ataaagtgcc tagcccatca aggattgtag gctttcttgg ctgctaaagc
     1141 gtttgacgaa aaagtccaag aaggcgctga tattggttct ccatcatttt catctaccgg
     1201 tttactatag gtcaaccagg ccgtccttgc cggcgcttgg gacgcagacg atggcatcaa
     1261 atgcctcgct caaattcgtt tctacagagg cgctttggga ccgcattcgc tttgctttca
     1321 tgggggcatc tgtcaatgtc agacatgaat cctccgatat aggttgattt ttgatctctt
     1381 caacacttgg tgttgtccga ctagagagac ttatatggat cagtattctt gcactgacaa
     1441 tggtgccact caatcccaag gatcgaccta gttagaggcg tagatcaagc aattcaaaac
     1501 agcgcagaag cagaaggagg tggaaatatg ttccagggtg aaaggttaca tgcaactaac
     1561 gttgctctct aggatgtgcc tgctgtaacg tattgtccag aaaagacaaa cccccgttat
     1621 cgcttgcatc ttggcggagc tgcgaaaaac gggggccgtg acgaatcaca agagaagtct
     1681 atcacatgga tcatgccaaa caaccccacg ccgccaataa gcgcggtggt gcttgagaaa
     1741 gtgcgcacag cttgagctgt ttcgggagcg cctgccccgg aaaccctact attccgacga
     1801 gcttacaacg ggcctacgga ttgcggatgt tgcccgcgcc cttggtgcgc gttacatcca
     1861 gccgaacggg ccaacgcacc gccactggat cgtttttgac gtagaccatg cagccgctac
     1921 gttatcttgg gatgacgtag gcgctccagc ccctaacatt accgtcacga acaaggccaa
     1981 cggccacgcg catctgatct acggactgga tacgccgatc cgcactgccc cggatggaaa
     2041 tgcagcccct ctgcggtatg ccgccgccat cgaggccgcg ctccgggaga aattaggggc
     2101 ggatatgggc tactccgggc tgatctgcaa gaaccccctg catgagcact ggctggtgca
     2161 ggtatgggaa ccccggctct acgatttagc ctggctatcc gattacctcg atctgagctc
     2221 ctacaatggc cgcaaatcgc ttcctgagta cgggcttggg cgaaactgta ccctgttcga
     2281 gaaaacgcgc ctgtgggcct ataaagcgat ccggcagggc tggccggatt accccgaatg
     2341 gttggccgct tgtgttgacc gggcatcagg atataacgcc cagttcgagc agccgctccc
     2401 cgcaaacgaa gtccggcaca cggccaagtc cattgccaaa tggacgcacc agcacctaag
     2461 ccccgcaggg ttcagagagg agcaggcccg gagaggtgcg aagggcggca aggtatccaa
     2521 gggaggggga aggccgagca atagcggtaa ggacaagtcc gatctattgc cggaggttct
     2581 gcggcttaag gctcaggggt acaccaaccg cgatatagcg gacgatcttc aaatctcccc
     2641 cagtacggtg agcgtttacc taaagcgtga tcgtccgtga tcgaaacaag ccttatcaga
     2701 taatagcccc ctttaggggg gttcctgcgc tcttgtcttt tagccttaaa aaatcctttt
     2761 agcgcggatc tttggcgagg tggacaactg cgcctgtccg tccatcgccc ccctgttttt
     2821 tcggggggtg tgggcggcag cagttatcca cggagccgct ctttcccttg atcttgctct
     2881 agggcttgca gtatcccgcc catggttgaa taatgtacta agcaaggcta cacagaggcc
     2941 cctctacctg cttcgcggtc tcgggcctct gtggccttgt tcaggggtgc tacgcccccc
     3001 cgaaaccccc tgtggccccc gctcgatccg ccccttcggg acggtctgcg cggtggcttc
     3061 ttggctctgg agaatggacg aggccctatg tcaacaacac agatagagaa agattcgaga
     3121 aggaaggcag tcaatggcgt tatttctagt ttccaactag agggggaggc cataaccgat
     3181 gaattcaaaa ttctacttga agattatatc aatggaaaaa tttccaccga tgaggtgtta
     3241 gctatggtaa gtgacgaacc cacaacaacg gaatacgact acgaaatcac cccccgcact
     3301 ctcggtcttg gcgggggctg gaatctgcgt ttgctggaga acggcgaaga ggtcggcggg
     3361 ggtgttttcc ccttgccgga gcactgcgat tttcgggatg aaaaggcgct ccagacgcta
     3421 ctcgatagcc tctatgagga cgccttagcg gaggcctctg catggctggc cagccgttaa
     3481 agcgcctccg ctctatcaag atccgggtct cagatgcgga gcttgagcgc ctgcgggagc
     3541 tctgccccaa ggcccaactg gcggagtgga tgcgggagca atgtctcggt gttgcgcaac
     3601 cccagcgccg aacgccggcg cctaccgtag atccggcgct cttgcggcag ttggccggaa
     3661 tgggcaacaa cctgaaccag atcgcccggc gcgttaattc cggggagtgg gggccgttgg
     3721 atcggctgcg catcatagcg gagctgtccg ctataggccg cgagctggag gaactgcacc
     3781 atgatcatca aattccataa ccgtggggta ggccggggct ccgggcctgt tggctacttg
     3841 ttgggccgtg acgggaaccg ggaaggcgca acgctcgacc ggggcaaccc tgcggtgatt
     3901 gaagccctga ttgattccag tccctacgcc aaaaaataca cctctggcgt tctgtcgttt
     3961 gccgaggctg atttacccag ggcgaccaag gacaagctga tgagcgactt tgaaaaggcg
     4021 ctcctgcccg gactggaggg caaccagtac gctgttttgt gggtagagca ccgcgacaag
     4081 gggcggctgg agctgaattt tgtagtcccg aacatcgaat tgcagagcgg gaagcggctg
     4141 caaccctact tccacaaggc cgacaatccg cgcctcgatg cgtggcgaac cgtggtaaac
     4201 ggggagctga aattgcacga cccggacgat ccgttaaacc gtcaggccgt tatcacgcca
     4261 aaggatttgc cgagggggca caaggaggcc gctcaggcca ttacagacgg gcttttacgc
     4321 atggccggag caggggagat ccagagccgc gatgatgtgc gcaaagcctt gcagggtgcg
     4381 ggtttcagcg tggtacgcga aaccaaaaac agcctgagca ttgccgaccc ggacgggggg
     4441 cggaacatcc gattaaaggg ggtgctgtat gagcaagatt ttagacatgg cgaaggactt
     4501 cgagcagagc tcgaagcagc aggccaacgc tatcgagaag cgtctgaccg aagaattcac
     4561 gaggcacgag aaagctatct ccggggcgtt gaaatcaagc gaggagaaaa tgagcgccgc
     4621 tattgccgag cacagccaga aaatgggctg gctgttgctc aaaacgtgga tctggatgct
     4681 ggtcagcatc gtcctgttct tgagcgcaac gagcgggatc ctctggtatc agggggaatt
     4741 gatagcggag cggcaagcca ctctccaagc cttggcagag aagggcggca agctaaagct
     4801 gacccagtgc gggaagaaat tggagcacct ttgcgtgcag atctccgacc ccaatccaac
     4861 cgtttggggg aaggatacct attggatcgt gccgaagggg tactaaatga cgggattgga
     4921 gcgggaattg ctctgcgcgt tcgaggcttt gcagagcgca tacgagcagc agcacaaggc
     4981 atggcaggaa agctacgcga acttggcgag catgtacgag agcaccaaac aagaggccga
     5041 gatcagcagg gcagcagcag agcgattgac gaggcaggtg aacggcttaa cgcagcaagt
     5101 ggacggcttg agcaagcagc tggagggctt gaccgggcta gtcagcaagt tgcaaaagaa
     5161 agggaacaca ggctcctgaa ggcactggat cgaggctggg agcgtggaga cagatcgcga
     5221 ggctttgagc gttgacggaa caaaacggcc gtcagttata aaaaaaggaa agtgctatca
     5281 ctttcctttt atcggcggga attatcgttg agttcttcta tgccattttt catagtagta
     5341 actgtaatct tcatctctac caccagattc aatccagtca tcatactcag ggctattttt
     5401 gaaatctgaa tcagacatgc taactccttg ttattagttg gcttctatgg aagctagcgc
     5461 accgtgaagt tatgatcaac gatgtagtcc acatcattgt cgaaatgtgc gttcagctag
     5521 tcttcggtgt tttctgcttt atttctagcg gcgattctcg cgtctgccca tgcggcagac
     5581 tttttagcct gttctcgttg ttcttcggtt gaagactcgc tgttgagaac tttgttgctc
     5641 gtttgtttga ccaccgaatc caaacctgtt gttgctgcaa aatgagctag gttaacaact
     5701 cccttaccta acaacttggc actagcgatt gcggtttctt tagccccaaa agcatcagac
     5761 acctcctcaa gcgctttaga taggttaaaa aatcccatac tccccctaag aatatcatca
     5821 ataaaattga gtgttgctct gtggataact tgcagagttt attaagtatc attgcagcaa
     5881 agatgaaatc aatgatttat caaaaatgat tgaaaggtgg ttgtaaataa tgttacaatg
     5941 tgtgagaagc agtctaaatt cttcgtgaaa tagtgatttt tgaagctaat aaaaaacaca
     6001 cgtggaattt aggtaatagt taaagcttgt gcctgattgt tgccacaatt ggtcagcagt
     6061 ttgctaggga atgagagggc agagaatgat aagcttagca cttaatttgt atggatatat
     6121 tgcattaagc aaggggacgt tatgcgtgta ttagccttat cggctgtgtt tttggtggca
     6181 tcgattatcg gaatgccagc ggtagcaaag gaatggcaag aaaacaaaag ttggaatgct
     6241 cactttactg aacataaatc acagggcgta gttgtgctct ggaatgagaa taagcagcaa
     6301 ggatttacca ataatcttaa acgggcgaac caagcatttt tacccgcatc tacctttaaa
     6361 attcccaata gcttgatcgc cctcgatttg ggcgtggtta aggatgaaca ccaagtcttt
     6421 aagtgggatg gacagacgcg tgatatcgcc gcttggaatc gtgaccatga cttaattacc
     6481 gcgatgaagt actcagttgt gcctgtttat caagaatttg cccgccaaat tggtgaggca
     6541 cgtatgagta aaatgctgca cgccttcgat tatggcaatg acgggcaatg tagacagttt
     6601 ttggctcgat ggtggtattc gcatttcggc tacccagcaa atcgcttttt tacgcaagct
     6661 gtatcacaac aagctgcacg tttctgagcg tagtcagcgc atcgtgaaac aagccatgct
     6721 gaccgaagcc aatggcgact atattattcg ggctaaaacg ggatactcga ctagtatcga
     6781 acctaagatt ggctggtggg ttggttgggt tgaacttgat gataatgtgt ggttttttgc
     6841 gatgaatatg gatatgccca catcggatgg tttagggctg cgccaagcca tcacaaaaga
     6901 agtgctcaaa caggagaaaa ttattcccta gaagtggtta gcgcgtattt gtgtgaaata
     6961 gccgtcatat aagctgtaaa gatatatgga caaaatgctg atgctcgatg cgcttgtagt
     7021 taatggctca tacttaatcg gtcaggccta atcgacttaa gctccgcgcg ttaactttca
     7081 gcctgagcat tagcatcaca ttcctaaacg taaaaagcct agccaatttg ctaggctttt
     7141 ttgttatctc aatggccatg gtcactcaac atgggggtgt gaataaagga gtggcgatgt
     7201 cagtaaaggt gaatgccgct gcgataaagt gcctagccca tcaaggattg taggctttct
     7261 tggctgctaa agcgtttgac gaaaaagtcc aagaaggcgc tgatattggt tctccatcat
     7321 tttcatctac cggtttacta taggtcaacc aggccgtcct tgccggcgct tgggacgcag
     7381 acgatggcat caaatgcctc gctcaaattc gtttctacag aggcgctttg ggaccgcatt
     7441 cgctttgctt tcatgggggc atctgtcaat gtcagacatg aatcctccga tataggttga
     7501 tttttgatct cttcaacact tggtgttgtc cgactagaga gacttatatg gatcagtatt
     7561 cttgcactga caatggtgcc actcaatccc aaggatcgac ctagttagag gcgtagatca
     7621 agcaattcaa aacagcgcag aagcagaagg aggtggaaat atgttccagg gtgaaaggtt
     7681 acatgcaact aacgttgctc tctaggatgt gcctgctgta acgtattgtc cagaaaagac
     7741 aaacccccgt tatcgcttgc atcttggcgg agctgcgaaa aacgggggcc gtgacgaatc
     7801 acaagagaag tctatcacat ggatcatgcc aaacaacccc acgccgccaa taagcgcggt
     7861 ggtgcttgag aaagtgcgca cagcttgagc tgtttcggga gcgcctgccc cggaaaccct
     7921 actattccga cgagcttaca acgggcctac ggattgcgga tgttgcccgc gcccttggtg
     7981 cgcgttacat ccagccgaac gggccaacgc accgccactg gatcgttttt gacgtagacc
     8041 atgcagccgc tacgttatct tgggatgacg taggcgctcc agcccctaac attaccgtca
     8101 cgaacaaggc caacggccac gcgcatctga tctacggact ggatacgccg atccgcactg
     8161 ccccggatgg aaatgcagcc cctctgcggt atgccgccgc catcgaggcc gcgctccggg
     8221 agaaattagg ggcggatatg ggctactccg ggctgatctg caagaacccc ctgcatgagc
     8281 actggctggt gcaggtatgg gaaccccggc tctacgattt agcctggcta tccgattacc
     8341 tcgatctgag ctcctacaat ggccgcaaat cgcttcctga gtacgggctt gggcgaaact
     8401 gtaccctgtt cgagaaaacg cgcctgtggg cctataaagc gatccggcag ggctggccgg
     8461 attaccccga atggttggcc gcttgtgttg accgggcatc aggatataac gcccagttcg
     8521 agcagccgct ccccgcaaac gaagtccggc acacggccaa gtccattgcc aaatggacgc
     8581 accagcacct aagccccgca gggttcagag aggagcaggc ccggagaggt gcgaagggcg
     8641 gcaaggtatc caagggaggg ggaaggccga gcaatagcgg taaggacaag tccgatctat
     8701 tgccggaggt tctgcggctt aaggctcagg ggtacaccaa ccgcgatata gcggacgatc
     8761 ttcaaatctc ccccagtacg gtgagcgttt acctaaagcg tgatcgtccg tgatcgaaac
     8821 aagccttatc agataatagc cccctttagg ggggttcctg cgctcttgtc ttttagcctt
     8881 aaaaaatcct tttagcgcgg atctttggcg aggtggacaa ctgcgcctgt ccgtccatcg
     8941 cccccctgtt ttttcggggg gtgtgggcgg cagcagttat ccacggagcc gctctttccc
     9001 ttgatcttgc tctagggctt gcagtatccc gcccatggtt gaataatgta ctaagcaagg
     9061 ctacacagag gcccctctac ctgcttcgcg gtctcgggcc tctgtggcct tgttcagggg
     9121 tgctacgccc ccccgaaacc ccctgtggcc cccgctcgat ccgccccttc gggacggtct
     9181 gcgcggtggc ttcttggctc tggagaatgg acgaggccct atgtcaacaa cacagataga
     9241 gaaagattcg agaaggaagg cagtcaatgg cgttatttct agtttccaac tagaggggga
     9301 ggccataacc gatgaattca aaattctact tgaagattat atcaatggaa aaatttccac
     9361 cgatgaggtg ttagctatgg taagtgacga acccacaaca acggaatacg actacgaaat
     9421 caccccccgc actctcggtc ttggcggggg ctggaatctg cgtttgctgg agaacggcga
     9481 agaggtcggc gggggtgttt tccccttgcc ggagcactgc gattttcggg atgaaaaggc
     9541 gctccagacg ctactcgata gcctctatga ggacgcctta gcggaggcct ctgcatggct
     9601 ggccagccgt taaagcgcct ccgctctatc aagatccggg tctcagatgc ggagcttgag
     9661 cgcctgcggg agctctgccc caaggcccaa ctggcggagt ggatgcggga gcaatgtctc
     9721 ggtgttgcgc aaccccagcg ccgaacgccg gcgcctaccg tagatccggc gctcttgcgg
     9781 cagttggccg gaatgggcaa caacctgaac cagatcgccc ggcgcgttaa ttccggggag
     9841 tgggggccgt tggatcggct gcgcatcata gcggagctgt ccgctatagg ccgcgagctg
     9901 gaggaactgc accatgatca tcaaattcca taaccgtggg gtaggccggg gctccgggcc
     9961 tgttggctac ttgttgggcc gtgacgggaa ccgggaaggc gcaacgctcg accggggcaa
    10021 ccctgcggtg attgaagccc tgattgattc cagtccctac gccaaaaaat acacctctgg
    10081 cgttctgtcg tttgccgagg ctgatttacc cagggcgacc aaggacaagc tgatgagcga
    10141 ctttgaaaag gcgctcctgc ccggactgga gggcaaccag tacgctgttt tgtgggtaga
    10201 gcaccgcgac aaggggcggc tggagctgaa ttttgtagtc ccgaacatcg aattgcagag
    10261 cgggaagcgg ctgcaaccct acttccacaa ggccgacaat ccgcgcctcg atgcgtggcg
    10321 aaccgtggta aacggggagc tgaaattgca cgacccggac gatccgttaa accgtcaggc
    10381 cgttatcacg ccaaaggatt tgccgagggg gcacaaggag gccgctcagg ccattacaga
    10441 cgggctttta cgcatggccg gagcagggga gatccagagc cgcgatgatg tgcgcaaagc
    10501 cttgcagggt gcgggtttca gcgtggtacg cgaaaccaaa aacagcctga gcattgccga
    10561 cccggacggg gggcggaaca tccgattaaa gggggtgctg tatgagcaag attttagaca
    10621 tggcgaagga cttcgagcag agctcgaagc agcaggccaa cgctatcgag aagcgtctga
    10681 ccgaagaatt cacgaggcac gagaaagcta tctccggggc gttgaaatca agcgaggaga
    10741 aaatgagcgc cgctattgcc gagcacagcc agaaaatggg ctggctgttg ctcaaaacgt
    10801 ggatctggat gctggtcagc atcgtcctgt tcttgagcgc aacgagcggg atcctctggt
    10861 atcaggggga attgatagcg gagcggcaag ccactctcca agccttggca gagaagggcg
    10921 gcaagctaaa gctgacccag tgcgggaaga aattggagca cctttgcgtg cagatctccg
    10981 accccaatcc aaccgtttgg gggaaggata cctattggat cgtgccgaag gggtactaaa
    11041 tgacgggatt ggagcgggaa ttgctctgcg cgttcgaggc tttgcagagc gcatacgagc
    11101 agcagcacaa ggcatggcag gaaagctacg cgaacttggc gagcatgtac gagagcacca
    11161 aacaagaggc cgagatcagc agggcagcag cagagcgatt gacgaggcag gtgaacggct
    11221 taacgcagca agtggacggc ttgagcaagc agctggaggg cttgaccggg ctagtcagca
    11281 agttgcaaaa gaaagggaac acaggctcct gaaggcactg gatcgaggct gggagcgtgg
    11341 agacagatcg cgaggctttg agcgttgacg gaacaaaacg gccgtcagtt ataaaaaaag
    11401 gaaagtgcta tcactttcct tttatcggcg ggaattatcg ttgagttctt ctatgccatt
    11461 tttcatagta gtaactgtaa tcttcatctc taccaccaga ttcaatccag tcatcatact
    11521 cagggctatt tttgaaatct gaatcagaca tgctaactcc ttgttattag ttggcttcta
    11581 tggaagctag cgcaccgtga agttatgatc aacgatgtag tccacatcat tgtcgaaatg
    11641 tgcgttcagc tagtcttcgg tgttttctgc tttatttcta gcggcgattc tcgcgtctgc
    11701 ccatgcggca gactttttag cctgttctcg ttgttcttcg gttgaagact cgctgttgag
    11761 aactttgttg ctcgtttgtt tgaccaccga atccaaacct gttgttgctg caaaatgagc
    11821 taggttaaca actcccttac ctaacaactt ggcactagcg attgcggttt ctttagcccc
    11881 aaaagcatca gacacctcct caagcgcttt agataggtta aaaaatccca tactccccct
    11941 aagaatatca tcaataaaat tgagtgttgc tctgtggata acttgcagag tttattaagt
    12001 atcattgcag caaagatgaa atcaatgatt tatcaaaaat gattgaaagg tggttgtaaa
    12061 taatgttaca atgtgtgaga agcagtctaa attcttcgtg aaatagtgat ttttgaagct
    12121 aataaaaaac acacgtggaa tttaggtaat agttaaagct tgtgcctgat tgttgccaca
    12181 attggtcagc agtttgctag ggaatgagag ggcagagaat gataagctta gcacttaatt
    12241 tgtatggata tattgcatta agcaagggga cgtt
//
