LOCUS       NZ_CP090453             9533 bp    DNA     circular CON 08-DEC-2023
DEFINITION  Escherichia coli strain PT62 plasmid pPT62-9kb, complete sequence.
ACCESSION   NZ_CP090453
VERSION     NZ_CP090453.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN24648241
            Assembly: GCF_021441965.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9533)
  AUTHORS   Lu,X. and Li,R.
  TITLE     Coexistence of tet(X4), mcr-1 and blaNDM-5 in ST6775 Escherichia
            coli isolates of animal origin in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9533)
  AUTHORS   Lu,X. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2022) Colloege of Veterinary Medicine, Yangzhou
            University, Shuangqiao Street, YangZhou, JiangSu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; QitanTech nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/08/2023 10:05:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,839
            CDSs (total)                      :: 4,723
            Genes (coding)                    :: 4,444
            CDSs (with protein)               :: 4,444
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 279
            CDSs (without protein)            :: 279
            Pseudo Genes (ambiguous residues) :: 0 of 279
            Pseudo Genes (frameshifted)       :: 78 of 279
            Pseudo Genes (incomplete)         :: 186 of 279
            Pseudo Genes (internal stop)      :: 44 of 279
            Pseudo Genes (multiple problems)  :: 29 of 279
            Pseudo Genes (short protein)      :: 4 of 279
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP090453.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PT62"
                     /isolation_source="pigeon"
                     /db_xref="taxon:562"
                     /plasmid="pPT62-9kb"
                     /geo_loc_name="China: Jiangsu"
                     /lat_lon="31.91 N 120.26 E"
                     /collection_date="2021-06-17"
                     /collected_by="Xiaoyu Lu"
     gene            1..951
                     /locus_tag="LZ175_RS24045"
                     /old_locus_tag="LZ175_24035"
     CDS             1..951
                     /locus_tag="LZ175_RS24045"
                     /old_locus_tag="LZ175_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_016946646.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSHLHNGLRKTLHYALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
                     /name="replication initiation protein"
     gene            959..1372
                     /locus_tag="LZ175_RS24050"
                     /old_locus_tag="LZ175_24040"
     CDS             959..1372
                     /locus_tag="LZ175_RS24050"
                     /old_locus_tag="LZ175_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040090700.1"
                     /translation="MALVSISEASRLTNKSRTTVHRYISKGKLSICTDENGVKKIDTSE
                     LLRVFGAFKAVHHEQLDNVTDEQHVTPNRTGETSKTKQLEHEIEHLRQLVTAQQSHIDS
                     LKQAMLLIESKLPTTREPVTPPVGKKSWQFWKK"
                     /name="hypothetical protein"
     gene            complement(1608..6482)
                     /locus_tag="LZ175_RS24055"
                     /old_locus_tag="LZ175_24045"
     CDS             complement(1608..6482)
                     /locus_tag="LZ175_RS24055"
                     /old_locus_tag="LZ175_24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005204747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="WP_193131410.1"
                     /translation="MNALSALLTKIEQASPTQRDKGTTFENLCVQYFLHEPKYAELYSD
                     VLSYGGWVSQYGETVGITKKKDDGIDLVAVTKTGEFHAIQCKNYNQTKIAKKDIDSFLA
                     ASDKTYFTLRYIVASTDNWTEEAKNMLRDKAVPVTALSLTDLEQSALDWSQFDFDPAYK
                     PVMKAKKQLRPHQTPALEAVKRGLTTADRGKLIMACGTGKTFTSLRIAEAVAGRGKTVL
                     FLVPSLALLSQTLDEWTQDTLIDLRCFAVCSDSDVGKKNHDDNVVVGISDLKYPATTNA
                     NSLVKAFNQPDIFGSDKPPYMNVVFSTYHSVEVIHQAQKLGFPTFDFIICDEAHRTTGA
                     TFEGDDESAFVRIHDNAYIAGQKRLYMTATPRIFGDDAKETEGVTLCSMDDKSLYGDDL
                     YVITFSKAVQLGILCDYKVIVLAVEEKHVSRRIQSLLKDENNQLKVDDAAKIVGCWKAL
                     AKQGLAGSDGVHPDAMKRAVAFCQVIEKDFRGKTHKVSSKLITEMFGAVVEAYQASEIE
                     LLRETAPDKAIDPSLKLQCQVKHVDGSMNATEKKSKIEWLKADTDENVCRILSNVRCLS
                     EGVDVPSLDAVLFLTPRSSQVDVVQSVGRVMRRAEGKELGYVILPVVIPAGEEPENALN
                     NNQAYRVVWQVLNALRAHDDRFDAMINKLEFNGKDTSRMEVIAVADKVVKKTKRQTKKS
                     ELAGKARKSSGIGSAVSPVPEQFDIEFSVGEIERALYAKIVKKCGNRHHWEDWANDIAK
                     IARTHIDRIHGILENPGNQVEISAFRAFADELRDDLNNSVSDDEIIEMLAQHLITKPVF
                     DALFADYNFTDHNPMSMAMQNVLNTLQEHHLEKEASTLNSFYESVKMRADGITTAEGKQ
                     QIIVQLYDKFFRNAFPRMTERLGIVYTPVEVVDFIIHSVNDVLKKEFGQTLADKGVHIL
                     DPFTGTGTFITRLLQSGLIPSDKLAYKFKNEIHANEIVLLAYYIAAINIEAVYHSITEE
                     SEYTPFEGICLTDTFEMYEKDDLVSEVLVDNSERRKRQKALDIRVIIGNPPYSSGQESA
                     NDNNQNVKYPMLDKRIAETYAACTTATNKNALYDSYIRAIRWASDRIGEQGVIGFVTNG
                     GYIDSNSADGLRKCLAEEFSSLYFFHLRGNQRTSGEKSRQEGGKIFGSGSRAPIVIAIL
                     VKNPAATQSGQIYFHDIGDYLTREEKLEKISDFGSIDGIEKTHGWQKITPDAYNDWLNQ
                     RDDSFYDFIEIGNKKDKDSKSLFSNYSRGLETNRDAWCYNYSKEELSQNIDKTLRFYNS
                     EVDRFRKSLTDISVDNFIDFDAKKISWNRGLKSDLVKGKKYHFDKEKIRVCLYRPFSKS
                     WVYMDSGINAYLNQIPKIFPTKDADNFLIYMSGSGNSGKDFSAIMTREIPDLNMQHSGG
                     QGFPLYLYESISERKSAISDFGLDYFNDFYATNIISKEDIFYYVYGLLSSRDYLSRYAD
                     NLAKELPRIPRVKKLEDFWSFSKAGRELAELHVNYETVEPYKADIDTGSLSYSQLGKDD
                     FYVEKMKFAKKGEKDTVIYNNKIRIKNIPTEAYDYVVNGKPALEWVMERQGVSTHKDSG
                     IVNDANDWAVETMNNPRYPLELFLRIITVSLETQKIVNSLPKLDI"
                     /name="type ISP restriction/modification enzyme"
     gene            complement(6595..7179)
                     /locus_tag="LZ175_RS24060"
                     /old_locus_tag="LZ175_24050"
     CDS             complement(6595..7179)
                     /locus_tag="LZ175_RS24060"
                     /old_locus_tag="LZ175_24050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_010891053.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQAASSQLQQQSETLSR
                     VIRELDSASGNMTDTVRKSVSSALTQVEKELKQAGLAQQKPATEALNQAADTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVLASCVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRTTYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(7192..8307)
                     /gene="mobA"
                     /locus_tag="LZ175_RS24065"
                     /old_locus_tag="LZ175_24055"
     CDS             complement(7192..8307)
                     /gene="mobA"
                     /locus_tag="LZ175_RS24065"
                     /old_locus_tag="LZ175_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_016946648.1"
                     /translation="MASYHLSVKTGGKGSASPHADYIAREGKYAREKDSDLEHKESGNM
                     PAWAAHKPSEFWKAADTSERANGCTYREIEIALPRELKPEQRLELVRDFVQQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERINDGIHRDPEQYFKRANTKEPERGGAKKARFG
                     ETPTERKEYLVAQRERWADLQNKHLERYQHADRVDSRSLKAQGIDREPERHLGAGQVQR
                     FDTEQLQAILERREAERQVQQSRDERDSVIDVTTSLREALSERDTLTLKQELKSEPEQE
                     SHSGRTFDFEKEPDKLNALVSDAMKDIQEEIDLQSLVNDAMAEFQGIHQEMERQRERER
                     LAEKQRQQEKERQRLAEQIRQKPDKGWSFSR"
                     /name="mobA"
     gene            8471..8938
                     /gene="traD"
                     /locus_tag="LZ175_RS24070"
                     /old_locus_tag="LZ175_24060"
     CDS             8471..8938
                     /gene="traD"
                     /locus_tag="LZ175_RS24070"
                     /old_locus_tag="LZ175_24060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_016946647.1"
                     /translation="MNKSEKQEQWAAERVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSKTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTT"
                     /name="traD"
     Typing          776..951
                     /name="ColE10"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.435"
                     /coverage="100.0"
                     /evalue="9.330000000000001e-86"
     Typing          complement(7196..8307)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.07"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtctgaat tagttgtttt caaagcaaat gaactagcga ttagtcgcta tgacttaacg
       61 gagcatgaaa ccaagctaat tttatgctgt gtggcactac tcaaccccac gattgaaaac
      121 cctacaagga aagaacggac ggtatcgttc acttataacc aatacgctca gatgatgaac
      181 atcagtaggg aaaatgctta tggtgtatta gctaaagcaa ccagagagct gatgacgaga
      241 actgtggaaa tcaggaatcc tttggttaaa ggctttgaga ttttccagtg gacaaactat
      301 gccaagttct caagcgaaaa attagaatta gtttttagtg aagagatatt gccttatctt
      361 ttccagttaa aaaaattcat aaaatataat ctggaacatg ttaagtcttt tgaaaacaaa
      421 tactctatga ggatttatga gtggttatta aaagaactaa cacaaaagaa aactcacaag
      481 gcaaatatag agattagcct tgatgaattt aagttcatgt taatgcttga aaataactac
      541 catgagttta aaaggcttaa ccaatgggtt ttgaaaccaa taagtaaaga tttaaacact
      601 tacagcaata tgaaattggt ggttgataag cgaggccgcc cgactgatac gttgattttc
      661 caagttgaac tagatagaca aatggatctc gtaaccgaac ttgagaacaa ccagataaaa
      721 atgaatggtg acaaaatacc aacaaccatt acatcagatt cccacctaca taacggacta
      781 agaaaaacac tacactatgc tttaactgca aaaattcagc tcaccagttt tgaggcaaaa
      841 tttttgagtg acatgcaaag taagtatgat ctcaatggtt cgttctcatg gctcacgcaa
      901 aaacaacgaa ccacactaga gaacatactg gctaaatacg gaaggatctg aggttcttat
      961 ggctcttgta tctatcagtg aagcatcaag actaacaaac aaaagtagaa caactgttca
     1021 ccgttacata tcaaagggaa aattgtccat atgcacagat gaaaacggtg taaaaaagat
     1081 agatacatca gagcttttac gagtttttgg tgcattcaaa gctgttcacc atgaacagct
     1141 cgacaatgta acagatgaac agcatgtaac acctaataga acaggtgaaa ccagtaaaac
     1201 aaagcaacta gaacatgaaa ttgaacacct gagacaactt gttacagctc aacagtcaca
     1261 catagacagc ctgaaacagg cgatgctgct tatcgaatca aagctgccga caacacggga
     1321 gccagtgacg cctcccgtgg ggaaaaaatc atggcaattc tggaagaaat agcgctttca
     1381 gccggcaaac cggctgaagc cggatctgcg attctgataa caaactagca acaccagaac
     1441 agcccgtttg cgggcagcaa aacccgtact tttggacgtt ccggcgggtt tttgtggcga
     1501 gttgtgttcg ggcggtgcgc gcaagatcca ttatgttaaa aacgacagga ttcatgaaaa
     1561 tcatcagaat tgtgcaggat ggtttttcca tcctgcacac ctatcgatta gatatctaat
     1621 ttaggcaggc tattgacgat tttctgagtt tccaggctga cagtgataat tctcaggaat
     1681 agctctagtg ggtatctagg gttattcatt gtttcaactg cccagtcatt agcatcattc
     1741 acaataccgc tatctttatg agtagagaca ccttgccgct ccatcaccca ttcaagagct
     1801 ggcttgccat tcacaacgta gtcataggct tcagttggaa tgttttttat gcgaatttta
     1861 ttgttataga tgactgtatc tttttcacct tttttggcga acttcatctt ctcaacataa
     1921 aaatcgtcct tgcctaactg ggaatagctc agggagcctg tatcaatatc agctttgtag
     1981 ggctcaactg tttcgtagtt tacatgcaac tcagcaagtt cacgacctgc cttagaaaat
     2041 gaccaaaaat cttctagttt ttttactcga gggatgcgcg gtaactcttt agctaaatta
     2101 tcagcataac gagacaaata atccctactg cttaatagtc cataaacata ataaaaaata
     2161 tcttctttac taataatatt agttgcataa aaatcattga agtaatctaa cccaaaatca
     2221 ctaatagcgc tttttctctc actaatactt tcatataaat acaatgggaa accttgccca
     2281 ccagagtgct gcatatttaa gtctggtatt tctcttgtca ttatagcgga gaaatccttt
     2341 ccactattac cactcccact catgtaaata agaaagttat cagcatcttt tgttgggaaa
     2401 attttaggga tctggtttaa atatgcatta atccctgagt ccatgtacac ccaagacttg
     2461 ctgaatggcc tgtacaaaca aactcttatt ttttctttat cgaaatgata ttttttacct
     2521 tttactaggt cagatttaag accacgattc caacttattt tctttgcatc aaaatcaata
     2581 aagttatcaa cactgatatc tgttaatgat tttctaaagc gatcaacctc agagttataa
     2641 aaccttaatg ttttgtctat attttgtgat agttcctcct ttgagtaatt gtaacaccat
     2701 gcatctctgt tagtttccag ccctcttgag taattagaaa aaagagattt gctatctttg
     2761 tcttttttat ttccaatttc aatgaaatca taaaaactgt catcacgctg attcagccaa
     2821 tcgttgtagg catctggtgt gattttctgc cagccatgcg ttttctcaat accgtcaata
     2881 ctgccaaaat cggaaatctt ctcaagcttt tcctcacgag tcagatagtc accaatatca
     2941 tggaaataaa tctgaccgga ctgtgttgca gccggatttt ttaccagaat cgcaatcaca
     3001 ataggcgcac gactaccgct gccaaaaatc ttaccgcctt cctgccgtga tttttctcca
     3061 gaggttcgct ggttccccct caaatggaaa aaatacaggc tactgaactc ttcagccaga
     3121 cacttacgca acccgtcagc cgagttagag tcaatatagc caccattagt cacaaaaccg
     3181 attacacctt gctcaccgat acggtcactc gcccagcgaa tagctcgaat ataggagtca
     3241 tagagtgcat tcttgttggt cgcagtggta caagcggcat acgtctctgc aatccgctta
     3301 tcgagcatcg gatattttac attctggtta ttatcgttcg ccgattcctg accggatgag
     3361 taaggcggat tcccgataat cacccgaata tccagagcct tctgccgttt acgacgttca
     3421 ctgttatcta ccagaacctc actgaccaga tcatccttct catacatttc aaaggtatct
     3481 gtcaggcaaa tcccttcaaa tggcgtgtac tctgattctt ccgtgatgct gtgatacacc
     3541 gcctcaatat taatggcggc aatgtaatag gctaacaaga cgatttcgtt ggcatggatt
     3601 tcatttttaa acttgtaagc cagtttatct gatgggataa gaccactttg cagcaggcgg
     3661 gtgataaacg taccggttcc ggtgaatggg tcaagaatat gcacaccttt gtcggcgagt
     3721 gtttgtccga actccttttt cagcacgtca ttcacgctgt gaataataaa atcaaccacc
     3781 tcaacaggcg tatacacaat tcccagacgc tcagtcattc tcgggaacgc gtttctgaaa
     3841 aacttatcgt acaactgtac gataatctgc tgtttacctt ctgcggtggt aatgccgtct
     3901 gcacgcattt ttaccgactc atagaagctg ttcagcgtgc tggcttcttt ttcgagatga
     3961 tgctcctgta acgtgttaag cacgttctgc atcgccatac tcatcgggtt atggtcggta
     4021 aaattgtaat cggcaaacag ggcatcaaag accggcttgg ttatcagatg ctgtgccagc
     4081 atttcaatga tttcatcgtc ggaaacactg ttattcagat catcacgcag ttcatcggca
     4141 aatgccctga aggcactgat ttctacttga ttacccggat tttcgagaat gccgtgaatg
     4201 cggtcgatat gcgtgcgggc aattttagcg atatcgttcg cccagtcttc ccagtgatgc
     4261 cggttaccgc attttttaac gattttcgcg tacagggcac gttcaatctc accaacggaa
     4321 aactcaatat caaactgctc aggaacagga ctcacggctg aaccaatgcc actgctttta
     4381 cgcgcttttc ctgccagttc agattttttc gtctgccgtt tagttttctt caccacttta
     4441 tcggctaccg cgatcacttc cattctgctg gtatctttac cgttaaactc cagcttgttg
     4501 atcatggcat caaagcggtc atcatgcgcc ctgagtgcat tcagcacctg ccagacaaca
     4561 cggtaggcct gattgttgtt taaggcgttt tccggctctt ctccggcggg aatgacaacc
     4621 ggcagaatga catagcccag ctctttacct tcggcacgcc gcataacacg cccgacagac
     4681 tgcaccacat ccacctgaga gcttctcggc gttaaaaaca acactgcatc cagagaaggc
     4741 acatccacac cttcagataa acaacggacg ttagacaata tccggcagac attttcatcc
     4801 gtatccgctt tcagccattc aattttggat tttttctcag tggcattcat gctgccgtca
     4861 acgtgcttca cctgacactg aagttttaat gacggatcaa tagccttatc cggtgcggtt
     4921 tccctgagaa gttcaatctc ggatgcctga taggcttcaa ccactgctcc gaacatttca
     4981 gtgatcagtt tagaactgac cttatgcgtt ttgccgcgaa agtctttttc tatcacctga
     5041 cagaacgcca ctgcccgctt catcgcatca ggatgaacgc catcagaccc cgccagcccc
     5101 tgctttgcca gtgctttcca gcaacccaca atttttgccg catcatcgac tttgagctga
     5161 ttattttcat ccttcagcag agactgaatg cggcggctga catgcttttc ttccacggca
     5221 agcacaataa ctttgtaatc acacaaaatg cccaactgaa cggctttaga gaaggtaatg
     5281 acatacaggt catcaccgta aaggcttttg tcatccattg agcataaggt gaccccttca
     5341 gtctctttcg catcgtcccc gaaaatgcgc ggagtcgcgg tcatgtacag gcgtttttga
     5401 ccggcgatat aggcgttgtc gtgaatacga acaaaggcgg actcatcatc cccttcaaag
     5461 gtcgcacctg tggtccggtg agcttcatca caaatgatga aatcaaatgt aggaaaaccc
     5521 agtttttgcg cctgatggat gacctcaaca gagtggtagg tagaaaacac cacgttcata
     5581 taaggcggct tatcactgcc gaagatatca ggctgattaa acgctttgac gaggctgttc
     5641 gcattcgtgg tcgccggata tttcaggtcg ctgataccaa caacgacatt gtcatcatgg
     5701 tttttcttac ccacatcact gtcagagcag acagcaaaac agcgcaggtc tatcagcgta
     5761 tcctgtgtcc attcatccag cgtctgactc aacaatgcca gactgggcac aaggaacagt
     5821 acggttttcc cccgtccggc aaccgcttca gcgatacgca gcgaggtgaa ggttttccct
     5881 gtgccgcacg ccataataag cttgccgcgg tcggcagtcg tcagccctct tttcacggct
     5941 tcaagcgcgg gagtctgatg aggtcggagt tgtttttttg ctttcatcac cggtttgtag
     6001 gccggatcaa aatcgaattg tgaccagtcc agtgcgcttt gctcaaggtc tgtcaggctt
     6061 aacgcagtga caggaacggc cttgtcccgc agcatatttt tagcttcctc cgtccagtta
     6121 tccgtagagg caacgatata gcggagcgta aaatacgttt tatcggaggc agccagaaaa
     6181 ctatcaatat cctttttggc gattttagtc tgattgtagt ttttgcactg gatagcatga
     6241 aactcccccg ttttggttac ggcgaccaaa tcaatgccat catctttttt cttcgtgatc
     6301 ccaacggttt caccgtactg agaaacccat ccgccatagc tcagcacgtc actgtacagc
     6361 tcggcatatt taggttcatg gaggaaatac tgcacacaaa ggttttcaaa ggttgtacct
     6421 ttgtcccgct gtgttggtga agcctgttca atttttgtca gcaatgcaga aagtgcattc
     6481 atcgttgata tccttaatgt cccttatttg ctggggttat cagatccccc cgacacgttt
     6541 aattaatgct ttctccgccg gagatcgacg cacagcgttc tgtgctctat gatgttattt
     6601 cttaataatc atccaggtat tctctttatc accatacgta gtgcgagtgt ccaccttaac
     6661 gcagggcttt ccgtcacagc gcgatatgtc agccagcggg gctttctttt gccagaccgc
     6721 ttccatcctc tgcatttcag caatctggct atacccgtca ttcataaacc acgtaaatgc
     6781 cgtcacgcag gaagccagga cgaagaatat cgtcagtaca agataaatcg cggatttcca
     6841 cgtatagcgt gacatctcac gacgcatttc atggatcatc gctttcgccg tatcggcagc
     6901 ctgattcagc gcttctgtcg ccggtttctg ctgtgctaat ccggcttgtt tcagttcttt
     6961 ctcaacctga gtgagcgcgg aactcaccga tttcctgacg gtgtcagtca tattaccgga
     7021 cgcgctgtcc agctcacgaa tgaccctgct cagcgtttca ctttgctgct gtaattgtga
     7081 tgaggcggcc tgaaactgtt ctgtcagaga agtaacacgc ttttccagcg cctgatgatg
     7141 cccgataagg gcggcaattt gtttaatttc gtcgctcata caaaatcctg cctatcgtga
     7201 gaatgaccag cctttatccg gcttctgtcg tatctgttcg gcgagtcgct gtcgttcttt
     7261 ctcctgctga cgctgttttt ccgccagacg ttcgcgctct ctctgccttt ccatctcctg
     7321 atgtatcccc tggaactccg ccatcgcatc gttaacaagg gactgaagat cgatttcttc
     7381 ctgtatatcc ttcatggcat cactgaccag tgcgttcagc ttgtcaggct ctttttcaaa
     7441 atcaaacgtt ctgccggaat gggattcctg ctcaggctca gacttcagct cctgttttag
     7501 cgtcagagta tccctctcgc tgagggcttc ccgtaacgag gtagtcacgt caattacgct
     7561 gtcacgttca tcacgggact gctgcacctg cctttcagcc tccctgcgct caagaatggc
     7621 ctgtagctgc tcagtatcga atcgctgaac ctgacccgcg cccagatgcc gctcaggctc
     7681 acggtcaatg ccctgcgcct tcagggaacg ggaatcaacc cggtcagcgt gctgataccg
     7741 ttcaaggtgc ttattctgga ggtcagccca gcgttccctc tgggcaacaa ggtattcttt
     7801 gcgttcggtc ggtgtttccc cgaaacgtgc cttttttgcg ccaccgcgct ccggctcttt
     7861 ggtgttagcc cgtttaaaat actgctcagg gtcacggtga ataccgtcat taatgcgttc
     7921 agagaacatg atatgggcgt ggggctgctc gccaccggct atcgctgctt tcggattatg
     7981 gatagcgaac tgataggcat ggcggtcgcc aatttcctgt tggacaaaat cgcggacaag
     8041 ctcaagacgt tgttcgggtt ttaactcacg cggcagggca atctcgattt cacggtaggt
     8101 acagccgttg gcacgttcag acgtgtcagc ggctttccag aactcggacg gtttatgcgc
     8161 tgcccacgcc ggcatattgc cggactcctt gtgctcaagg tcggagtctt tttcacgggc
     8221 atactttccc tcacgcgcaa tataatcggc atgaggagag gcactgcctt ttccgccggt
     8281 ttttacgctg agatgatagg atgccatcgt gttttatccc gctgaaggcg cgcaccgttt
     8341 ctgaacgaag tgaagaaacg tctaagtgcg ccctgataaa taaaagagtt atcagggatt
     8401 gtagtgggat ttgacctcct ctgccatcac tgagcataat cattccgtta gcattcagga
     8461 ggtaaacagc atgaataaaa gcgaaaaaca ggaacaatgg gcagcagaaa gagtgcagta
     8521 tattcgcggc ttaaagtcgc cgaatgagca acagaaactt atgctgatac tgacggataa
     8581 agcagataaa acagcacagg atatcaaaac gctgtccctg ctgatgaagg ctgaacaggc
     8641 agcagagaaa gcgcaggaag ccagagcgaa agtcatgaac ctgatacagg cagaaaagcg
     8701 agccgaagcc agagccgccc gtaaagcccg tgaccatgct ctgtaccagt ctgccggatt
     8761 gcttatcctg gcgggtctgg ttgacagtaa gacgggtaag cctgttgatg ataccgctgc
     8821 cttactgggt gcattagcca gtctgaatga cctgtcacgg gataatccga agtggtcaga
     8881 ctggaaaatc agagggcagg aactgctgaa cagcaaaaag tcagatagca ccacatagca
     8941 gacccgccat aaaacgccct gagaagctcg tgacgggctt ttcttgtatt atgggtagtt
     9001 tccttgcatg aatccataaa aggcgcctgt agtgccattt acccccattc cctgccagag
     9061 ccgtgagcga agcgaactga ttttcacaga gataacagcg attcaggtgc cggacggtcg
     9121 gagacaaaag gaatgttcag cgggttgccc gagcttgcga gggtgctatc tgagtgtttt
     9181 tgggggttaa ggtctgtttt gaagagaagc aaacagcgtt tgcgacatcc ttttatcata
     9241 tcgctcaagt gactcaggcg tgcagttatc cacaaggctg ggatctattc ttttttatct
     9301 ttttatattc tttctttatt ctataaatta taaccaattg aatataaaca aaaaaaacac
     9361 acaaaggtct agcgaaattt acagagggtc tagcagaatt tacaagtttt ccagcaaagg
     9421 tctagcagaa tttacaaata cccacaactc aaaggaaaag gactagtaat tatcattgac
     9481 tagaccatct caattggtat agtgattaaa atcacctaga ccaattgaga tgt
//
