LOCUS       NZ_CP090437             5596 bp    DNA     circular CON 08-DEC-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain KPN857 plasmid pE,
            complete sequence.
ACCESSION   NZ_CP090437
VERSION     NZ_CP090437.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN24571286
            Assembly: GCF_021442005.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Lv,F., Guo,S.Z. and Huang,Y.
  TITLE     the WGS of hypervirulent Carbapenem resistant klebsiella pneumoniae
            KPN857
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Lv,F., Guo,S.Z. and Huang,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2022) Clinical Laboratory, Dongguan Donghua
            Hospital, 1 Kefa 7th Road, Songshan Lake Management Committee,
            Dongguan, Guangdong 523000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 203.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/08/2023 09:57:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,888
            CDSs (total)                      :: 5,766
            Genes (coding)                    :: 5,590
            CDSs (with protein)               :: 5,590
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 64 of 176
            Pseudo Genes (incomplete)         :: 121 of 176
            Pseudo Genes (internal stop)      :: 22 of 176
            Pseudo Genes (multiple problems)  :: 31 of 176
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP090437.1.
            Bacteria and source DNA available from China,Dongguan City,
            Guangdong Province.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN857"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pE"
                     /geo_loc_name="China: dongguan"
                     /lat_lon="22.925801 N 113.862947 E"
                     /collection_date="2021-05-01"
                     /collected_by="dongguan donghua hospital"
     gene            join(5444..5596,1..357)
                     /locus_tag="LZ608_RS29280"
                     /old_locus_tag="LZ608_29280"
     CDS             join(5444..5596,1..357)
                     /locus_tag="LZ608_RS29280"
                     /old_locus_tag="LZ608_29280"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            364..576
                     /locus_tag="LZ608_RS29285"
                     /old_locus_tag="LZ608_29285"
     CDS             364..576
                     /locus_tag="LZ608_RS29285"
                     /old_locus_tag="LZ608_29285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1243..1467
                     /locus_tag="LZ608_RS29290"
                     /old_locus_tag="LZ608_29290"
     CDS             1243..1467
                     /locus_tag="LZ608_RS29290"
                     /old_locus_tag="LZ608_29290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1451..1717
                     /locus_tag="LZ608_RS29295"
                     /old_locus_tag="LZ608_29295"
     CDS             1451..1717
                     /locus_tag="LZ608_RS29295"
                     /old_locus_tag="LZ608_29295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1787..2137)
                     /locus_tag="LZ608_RS29300"
                     /old_locus_tag="LZ608_29300"
     CDS             complement(1787..2137)
                     /locus_tag="LZ608_RS29300"
                     /old_locus_tag="LZ608_29300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2203..2748)
                     /locus_tag="LZ608_RS29305"
                     /old_locus_tag="LZ608_29305"
     CDS             complement(2203..2748)
                     /locus_tag="LZ608_RS29305"
                     /old_locus_tag="LZ608_29305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2774..3100)
                     /locus_tag="LZ608_RS29310"
                     /old_locus_tag="LZ608_29310"
     CDS             complement(2774..3100)
                     /locus_tag="LZ608_RS29310"
                     /old_locus_tag="LZ608_29310"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(3458..3616)
                     /locus_tag="LZ608_RS29485"
     CDS             complement(3458..3616)
                     /locus_tag="LZ608_RS29485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4256..4432
                     /locus_tag="LZ608_RS29315"
                     /old_locus_tag="LZ608_29315"
     CDS             4256..4432
                     /locus_tag="LZ608_RS29315"
                     /old_locus_tag="LZ608_29315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4422..4766
                     /locus_tag="LZ608_RS29320"
                     /old_locus_tag="LZ608_29320"
     CDS             4422..4766
                     /locus_tag="LZ608_RS29320"
                     /old_locus_tag="LZ608_29320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4948..5193)
                     /locus_tag="LZ608_RS29325"
                     /old_locus_tag="LZ608_29325"
     CDS             complement(4948..5193)
                     /locus_tag="LZ608_RS29325"
                     /old_locus_tag="LZ608_29325"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     Typing          4091..4170
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(3508..3905)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          4..692
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.565"
                     /coverage="99.0"
                     /evalue="6.72e-123"
     Typing          4757..5595
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atccgcgccc acgagcaggg tatgagcgcg gccatgcagt cgaaccgcct gagcgtgatg
       61 cgtatggtcg ggcggacatg gctgaccatc acgatggtgt caggactgct gttcgccagc
      121 ctgagcgggg tgttgtggta tcaggggagc ttgatagcgt caaatctggc cgaaatcgac
      181 cggcagaacg cagcgctgtc gaagctgaac gcgaagacct ggggcgtgac gtacctggaa
      241 gacagcaacg gacgttttct ggtgctgccg aaggggacgg ccgtagacag gacgcagagc
      301 tggacggtcg ggaacgggcg gtcgaagcag aacgcgctga gactggtgaa ggagtaagtg
      361 ccgatgaccg agctggaaaa acagttgctg agcgcattag agcagctaca gcaggactac
      421 tcgaaaaggc tggacgagtg ggagagcgcc ttcggggaat ggcggaaaat gtcagggctt
      481 atgcaacggg agaacgcggc gctgagcgag cgcgtgacgc gcttgagtca gcaggtggaa
      541 cgcttgagcg ggcaactgca gcgtttgagc cggtagtgca gcgtcatgag atggccgtgg
      601 ccgcagaacg ggcgcacgag caacggcagc acgaaaagga actgacggaa gcgcgttccc
      661 ggcagcgaag ctatgacggt ccgtcgctgg gctgaaatgc aggaaaacag ggttctcatc
      721 gggcatttac gctgaaaccg gatgtcattc acctggctgc atggctatgc agccaggtaa
      781 aatttatctg tcgcgaacgg ggcggtttat cgggtggttt gttgccggtt ttacctgact
      841 gccaccccgt tcgcggcgaa cccgtccggg gcggtgcggg caacgggtgt tatgtttaat
      901 cagcgtgaac ggcagataca aaaaagcccc ggagggcttt taactactga cttatttttt
      961 tgaagatttg ctgggtttca atttgtcagt atttcccggc gtttttttat tgtcacgctg
     1021 gcgtttatca ggttcccctg ttgaatcggc aattcgtttc ggacccggct taagacccat
     1081 aatcgttatc ctttcaatag ttaagaggaa agccctcaag ctagatatat tgaccgcgtt
     1141 acggattatc aagaggggct aacaagatta ttttttgttc ttttaggttg attgtggtac
     1201 tgttgatata catgtatagc aaatggtgtt cgggaggttt atatgcttgc tatccggtta
     1261 tctgatgaga ttgagtcccg tctggactcg ctggcgaagc aaaccggcag aacaaagacg
     1321 ttttatgcgc gggaagcaat actggcgcat ctggaagacc tggaggatta ttatctttca
     1381 gcagaaactg ctgcacgcgt tcgccgtggt gatgaagcag tgcattcgtc tgaagacgtg
     1441 aggaagtcac ttggtctgga cgattaacta ttccgatcgg gcgctcaaat cgttacgcaa
     1501 gatggacaaa cagaacgcac gacggattgt ggattttatg agtttacgca ttgcagttgc
     1561 tgccgatcct cgccagtcag ggaagccgct caaaggtgag ctgggcgagt tctggcgcta
     1621 tcgggtggga gattatcgcg ttctgtgtga gatccgagat gacgagcttg ttatccttgc
     1681 cgccacgatt ggacatcgcc gcgaagttta cgactgaagc ccgctatgcg ggcttttttg
     1741 atgccgacaa caggtattat ggtaaatact cgactaacta cgtgttctac tgtttgtcgg
     1801 ttttagttaa ccagcaggtt gtcttaacga taatgtagcc gttattttta actaataagg
     1861 tcatattgtt aacgtcaggc ataataaaag acattatggc ctcagagtta tcatcagaag
     1921 cagctctctg cattactact tcgattccct tttctttgaa tctataagta tccagttgcc
     1981 tattaaactt agaaagacct acaaccatac ttagtttgtt atctttaaca gtaagagatg
     2041 cttcctgagt accattacat cggtagtaag cggtgtaacc gtcagcaaaa gatcgaccgg
     2101 ggaatataaa tgctaggaac gcaattaacc aatacatagt tgagaatctt ggtattacac
     2161 atttcactat cctctctttc atttttcgtt caccatactt tgttatctga tttttaattt
     2221 ttctatttta taaaccaagt ctctaccaaa aaggcgaaaa ttatcctcaa ataataataa
     2281 ttgtttaaga acatccatga aagcagctgc ttcaccctca gttatgggag ctactctata
     2341 taacatataa cctgagttaa gggtttcttt tatactttct tcaatgctca ttataaatat
     2401 tagtggatta tcatttttct tcccccatgg gtttctaatt aatccttcga tgtactcagg
     2461 tcctttgcgt cctattgcct gcgcaatatt ttttatttca tctgcgtgag ggctttgttg
     2521 tacagctttg tttatgcctg cttgagtgat tttcagagac tctacaccgt gtatgttgta
     2581 tgagataaaa tgtaataaat taagatgcaa tgcaattaat ccatttttgt ttacatctga
     2641 gtatttggat aaaaaacgta atgaagtgtt aactctccct tgaagcctaa caattgggag
     2701 tgctgccttg tatatttcct tctccgttaa ataccgttta aaaaacataa ttctccgcct
     2761 ccgtatagcg tgcctatttt atttcgtcgt aaaacttagc tctgaaatat tcctttagaa
     2821 ccatcatgtc attaataatt gaatctatct catccatttt ttcttggtat tcttttaatg
     2881 gtgaagggag cggtaactca atatttgaaa agtgattggc gagataggga tcaaggtttc
     2941 tcatccattc aggcttttcg ccaatgcgtt cttcaactga aagtgaacct gataatctct
     3001 ggttaataag atcacttaga ctcattttgg ttttattcga aagccgttgt aaggattgct
     3061 ttaatcctat atctagcatg atatttgttc tttctctcat taccccccct tgctattgcg
     3121 ttttttgttg cgcacaaagc taccattgcg cattaagaaa cgcaataaaa aaggagggag
     3181 ggagtggttt gtacatgatg tgcagtgcgc tgagggcatc agtggcgggt gtttttccgt
     3241 gggatggatg gagctccggg gataacagaa ctgacgatca tttaaaggat cttcttgaga
     3301 tccttttttt ctgcgcgtaa tctcttgcac tgtaaacgaa aaaaccgcct ggggaggcgg
     3361 tttgatcgaa ggttaagtca gttggggaac tgcttaaccg ggtaactggg tatacgagac
     3421 cgcagatacc aaaactgttc tttcagtgta gccgcagtta ggcacccact tcaagacctc
     3481 tcaatatctc tctcgtatat atccagttac caatggctgc tgccagtggc gtttttgcgt
     3541 gtccgtccgg gttggactca agatgatagt taccggaaag ggcgcagcag tcgggctgaa
     3601 cggggggttc gtgcatacag cccagcttgg agcgaacgac ctaaaccgag ccgagatacc
     3661 agcagcgtga gctatgagaa agcgccacgc ttcccgaaag ggaaaaaggc ggaacaggta
     3721 tccggtaaac ggcagggtcg gaacaggaga gcgcacgagg gagccgccgg agagaaatca
     3781 ccggcatctt taagtcctgt cgggtttcgt cctcctctga cttgagcgtc catttctgtg
     3841 atgctcgtca ggggaggcgg agcctatgga aaaacggcaa cgccgcatcc ttttctccgg
     3901 ccctttctga tttgagcgcc tttgctttat cccgttatcc gtgagtcggt cacgcccgcc
     3961 gcagccgaac ggcaggagcg cagctaccga gtgagcgagg aggcgtcatt tcatctgtcc
     4021 gtgcatggta ttgcggacgc tcctgagcca catatcacac ctgccacatg aggcactttc
     4081 atgaatcgtg ctctcatgcc aacatagtaa gccagtatac acagcgtaaa cgctgtgact
     4141 ggttcagggc tgcgccccga aacccgctaa caccactgac gcgctggcgc ttgtccaacg
     4201 ccggagcgac ccggaaaact tttccgtgcc gtccggcgtt tgcgaaggag ggataatgca
     4261 gaagccgaag aagaagccgc tcgtcgtaaa aaaaagatcc tggcatccgg cagtgatggc
     4321 aaatctgatg cgtcaggcac aggaggaaga ttaccgccgg gagcagatgg cagagcttga
     4381 gcgttcagcc cggtcacatt ttgcggatat cggaggagag gatggcggtt aagcgtaccc
     4441 gttttttagg tatccgggtg accgatgggg agtaccagca gctgctggag cgctgcaatg
     4501 gaaggcaact ggcggtgtgg atgcgggaaa cctgtctcga tacgcgcccg gcgcggtcgt
     4561 tgcggctgcc gtccatcgat ccggttttgc tgcgccagct tgccgggatg gggaacaacc
     4621 tcaaccagat cgcccggaaa attaacggcg gccagtggtc aggcgctgac cgggttcagg
     4681 tggtggccgc gctgatggcc attgatgccg aactcgagcg gctgcggcat gccgtgctgg
     4741 aaaagggggc agacgatgat cgttaagttt catccgcgag ggcgaggcgg cggtgccggg
     4801 ccggtggatt atctgctggg gaaagaccgc cagcgcgacg gggccatcgt cctgcaggga
     4861 aagccggagg aggtccggga gcttatcgac gcctcgccct acgccaaaaa gtacacctcc
     4921 ggcgtcctgt cttttgctga acaggattta ccgcccggcc agcgtgaaaa gctgatggcg
     4981 agcttcgagc gggttctgat gcccggactc gataaagacc agtacagcgt gctgtgggtt
     5041 gaacaccggg acaaggggcg gctggaactg aattttctgg taccgaacac ggaactgctg
     5101 accggcaggc gtctccagcc gtactacgac cgggcagacc gtccgcgcat cgatgcgtgg
     5161 cagaccgtgg tgaacgggcg actggggctg cacgacccga acgcaccgga gaaccggcgc
     5221 gcgctggtga cgccatcttc gttacccaaa acgaagcagg aagccgcaga ggctattacg
     5281 cggggcttac tggccctcgc ctcgtcaggg gagcttaaaa cgcgtcagga cgtcactgag
     5341 gcgctggaaa gcgcaggttt tgaggtcgtg cgcaccacaa agagcagcat cagcattgcc
     5401 gacccggacg gagggcgaaa catccgactt aagggagcca tctatgaaca gtcttttaac
     5461 gctggcgaag gacttagagc agaaatcgaa agcgcagcag cagagtaccg gcgaaatgct
     5521 gaaagccgca ttcagcgagc acgagaagtc tgtcagagcg gaactgagcg aaagcgggaa
     5581 gagaatcagc gccgcc
//
