LOCUS       NZ_CP090436            11970 bp    DNA     circular CON 08-DEC-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain KPN857 plasmid pD,
            complete sequence.
ACCESSION   NZ_CP090436
VERSION     NZ_CP090436.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN24571286
            Assembly: GCF_021442005.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Lv,F., Guo,S.Z. and Huang,Y.
  TITLE     the WGS of hypervirulent Carbapenem resistant klebsiella pneumoniae
            KPN857
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Lv,F., Guo,S.Z. and Huang,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2022) Clinical Laboratory, Dongguan Donghua
            Hospital, 1 Kefa 7th Road, Songshan Lake Management Committee,
            Dongguan, Guangdong 523000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 203.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/08/2023 09:57:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,888
            CDSs (total)                      :: 5,766
            Genes (coding)                    :: 5,590
            CDSs (with protein)               :: 5,590
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 64 of 176
            Pseudo Genes (incomplete)         :: 121 of 176
            Pseudo Genes (internal stop)      :: 22 of 176
            Pseudo Genes (multiple problems)  :: 31 of 176
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP090436.1.
            Bacteria and source DNA available from China,Dongguan City,
            Guangdong Province.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN857"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pD"
                     /geo_loc_name="China: dongguan"
                     /lat_lon="22.925801 N 113.862947 E"
                     /collection_date="2021-05-01"
                     /collected_by="dongguan donghua hospital"
     gene            complement(join(10371..11970,1..86))
                     /locus_tag="LZ608_RS29210"
                     /old_locus_tag="LZ608_29210"
     CDS             complement(join(10371..11970,1..86))
                     /locus_tag="LZ608_RS29210"
                     /old_locus_tag="LZ608_29210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(191..1678)
                     /gene="ltrA"
                     /locus_tag="LZ608_RS29215"
                     /old_locus_tag="LZ608_29215"
     CDS             complement(191..1678)
                     /gene="ltrA"
                     /locus_tag="LZ608_RS29215"
                     /old_locus_tag="LZ608_29215"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            2323..2568
                     /locus_tag="LZ608_RS29220"
                     /old_locus_tag="LZ608_29220"
     CDS             2323..2568
                     /locus_tag="LZ608_RS29220"
                     /old_locus_tag="LZ608_29220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            2842..3213
                     /gene="tolA"
                     /locus_tag="LZ608_RS29225"
                     /old_locus_tag="LZ608_29225"
     CDS             2842..3213
                     /gene="tolA"
                     /locus_tag="LZ608_RS29225"
                     /old_locus_tag="LZ608_29225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            3210..3575
                     /locus_tag="LZ608_RS29230"
                     /old_locus_tag="LZ608_29230"
     CDS             3210..3575
                     /locus_tag="LZ608_RS29230"
                     /old_locus_tag="LZ608_29230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            3750..4028
                     /locus_tag="LZ608_RS29235"
                     /old_locus_tag="LZ608_29235"
     CDS             3750..4028
                     /locus_tag="LZ608_RS29235"
                     /old_locus_tag="LZ608_29235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            4016..4330
                     /locus_tag="LZ608_RS29240"
                     /old_locus_tag="LZ608_29240"
     CDS             4016..4330
                     /locus_tag="LZ608_RS29240"
                     /old_locus_tag="LZ608_29240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4494..4922
                     /locus_tag="LZ608_RS29245"
                     /old_locus_tag="LZ608_29245"
     CDS             4494..4922
                     /locus_tag="LZ608_RS29245"
                     /old_locus_tag="LZ608_29245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            4948..5127
                     /locus_tag="LZ608_RS29250"
                     /old_locus_tag="LZ608_29250"
     CDS             4948..5127
                     /locus_tag="LZ608_RS29250"
                     /old_locus_tag="LZ608_29250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5154..5684)
                     /locus_tag="LZ608_RS29255"
                     /old_locus_tag="LZ608_29255"
     CDS             complement(5154..5684)
                     /locus_tag="LZ608_RS29255"
                     /old_locus_tag="LZ608_29255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5691..6422)
                     /gene="mobC"
                     /locus_tag="LZ608_RS29260"
                     /old_locus_tag="LZ608_29260"
     CDS             complement(5691..6422)
                     /gene="mobC"
                     /locus_tag="LZ608_RS29260"
                     /old_locus_tag="LZ608_29260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(6422..8386)
                     /locus_tag="LZ608_RS29265"
                     /old_locus_tag="LZ608_29265"
     CDS             complement(6422..8386)
                     /locus_tag="LZ608_RS29265"
                     /old_locus_tag="LZ608_29265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(9870..10019)
                     /locus_tag="LZ608_RS29270"
                     /old_locus_tag="LZ608_29270"
     CDS             complement(9870..10019)
                     /locus_tag="LZ608_RS29270"
                     /old_locus_tag="LZ608_29270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(10104..10361)
                     /locus_tag="LZ608_RS29275"
                     /old_locus_tag="LZ608_29275"
     CDS             complement(10104..10361)
                     /locus_tag="LZ608_RS29275"
                     /old_locus_tag="LZ608_29275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     BGC             5371..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          9189..9767
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5695..6422)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 gacgtaccgt tcacattccc actggcctga ccaccattat gtcccagacc tgaattaccc
       61 ggacctcgac cgtctccacc actcataccg ttttcctctt tgacatttaa aacaataaat
      121 taaagccaac atgtcgcggt agggataccc gttaccggat accccccgca cagatcccgg
      181 cgtgcgcgat ttacgcaccg ggctcctgcc tcgggtgtct ggcggtgaac cgctccacag
      241 gccatggatg aagaacccga acccttggta gccatgcggc tgccagtttg tttgctttcg
      301 tccaggtcgt atcatccttc tggctcctgc gcctgagcgc ccggcgccag aggtttgtta
      361 cgtgtgtcct gaacttctgc atggtgggga agttgcccgg taccgagtga tagttcaggt
      421 atccctgaac cactctcctg agccattttc cctgttcggg gattgagtaa tgccagcgcc
      481 ttcgcagacc gtctttgatg gctttcagag ttgccgtcat ccgatcccgg cgggtctttc
      541 gtatcagcat gaacctgccg ttgcgatctt tcccgctgat gtgcgtgaac ccgaggaagt
      601 tgaacgtttc tggtttgcct tttcccctga tggcacggtt ttcggcagcg aagcggccga
      661 actccatcag acgggttttc tccgggtgaa ccgtgagtcc gaactccctc agtctgcgct
      721 gcatggctat acggaagcgc cgggcatcgt atcgtttgtc gaacccgatg acgatgtcat
      781 cggcgtatct gaccattacc acattgcctg tggcatagcg acgtcgccac tgatgcgccc
      841 acagatcgaa gacgtagtgg aggtatatgt ttgccagcag cggtgagatg accgcaccct
      901 gtggggtgcc ttcctccgtt gctcgccatt gaccctcctc cgacgtcccg gctgtgagcc
      961 acttacgtat gagcctgatt accctccggt cgccgatccg atgctctgtg aacctgatca
     1021 gccattcgtg gctcaccctg tcgaagaact gactgatgtc ggcatccagt acccagttta
     1081 cgttagtgcg taccagccct gtggccagtg cgtccagtgc atcgtgctgg cttcgcccgg
     1141 gtctgaaccc gtatgagaac cccataaagt cgttttcata gactgcgttc aggattttca
     1201 ccagcgcata ctggacgatc ttgtcctcca gcgaggcgat gccgagcggg cgttgttttc
     1261 catccgcttt tgggatgtag tgacgcctgc cgggctgcgc cctgtagctg ccctgatgta
     1321 gcctccggtg cagatctgtt atgttgttct tcatgtttcc ggcgtagtcc atccacctga
     1381 tgccatccac tccggcggcc gctttcctgc tcagggagag gaatgcggct tccagtgctt
     1441 cgactgtcag caggtggaac aatgctgtaa accgttcttt cttccgctgc ttcgcagctt
     1501 cccgcacgcg tgacagcctc tgtgacatgc tttcccggct ctgtgtccgg cgcatgtgtg
     1561 gctgttccgc gttccccttg gccccgctcc ttcgctccac tgactccgct cctttcgggt
     1621 tgttcgcctg cttcgccgct actatgagcg agtccgactt ctcctctccg tacatcaccg
     1681 gctatgactc ctcgtcttcc cggtgcgggc catctccgac actggcagat ggtcagaggg
     1741 gagatctccc ggttcccgcg tagagatcgt attgacatgc cagggtctca gaccccgccg
     1801 ggtccatgtg gcactcgcag tgtcgcaccc tatgatgttg ccttccgtta acagtacaac
     1861 gtcggcaccc ggtaatttaa tatacatttc gtggctcaat ggctggcctg tcaacacccc
     1921 tgtcaacgct tcgccccata cctcgcggta tgcaacgcat gactcgggga ccttgtggat
     1981 tgctggtcct tcaatggtcg gggactttca ccccttgatc tctaaccggt ctcccggcgc
     2041 acactgtaca tatacacagt ataattatta ccttactcct cccgttttca ggtgtcaata
     2101 tatgagatta gttctcatct tcaggcacaa aaaagcgccc ggagccacct gtaagcagat
     2161 cgcgtctttt cccgaaaatt tctcatggca acaaatttta ttatttaaga cattcatcac
     2221 acttacattt cagatggtat agacacatct gaaagctaac tggcatgagg agaggacctg
     2281 cggttaaact gttcgggaaa cctgagacgc ccggaggcca ctatgacgca cctgaaactg
     2341 actcccgctg aagaaaagcg cctggcggac gcctttgtcg ctcaggcact ggccatgggc
     2401 ctgaaaggga acaaaacgct gccaaaggcg gtaaaacaac aggtgcggga aagggtgctt
     2461 cgtgccattc aggacgaacg tgaagccagg cgggcgcagg aaatgcgtcg ggagaaatac
     2521 gaggctacgg aaaccacctt cacctggcag ccacagcgcc gccggtaagg ggtatttacc
     2581 ataacgtcgc attgcccgca ccgctcagac ggtctgaggg cgtttccggc ggctacggga
     2641 taaaacaggc aacaaaccac ccggaaaacc gccggaaacg cgcccggtaa attgtaaccc
     2701 gctgcatgcc tatgcacgca actgcatggc gggccgtttg gtgtaaaaac gatacgataa
     2761 gccgtgttta tgtaaatgaa cgtggccgta aagagtgaca aatatacatg taacattttt
     2821 tactgacagg tcatcaaccc gatgcgttta atacccttta tcctgctggc attgtgcagc
     2881 cccttactcc tgacggcctg taagcattcc accacccagc cgccgaaaat ttatactctg
     2941 gataagtcat ctaaaatcag tagctacgct gcggccatca agaccgctgt tgaaatgcag
     3001 atgtttgaac cagaggccta tgcgggaaaa atgtgcaccg tgcggatatc catacaacgt
     3061 gatggcactc tgaacagcgc cattgctgaa ggaggagatc cgaaactctg taaagcagca
     3121 atatcagccg ttacccacgc caaaattccg ccggcaccgg atgaaaatac ctggcaaata
     3181 ttcaaaaacg cccctctgga tttcagacca tgaaagcttt tactctgaca gtgctggcgg
     3241 ccatgttcct cactccggta catgcgatac cactaaaacc cgtaaccaca ctgcagccaa
     3301 cctacccggt acaggccgca gcaaaacgca tcgaaggtca ggtgaccgcc acgtttgata
     3361 ttctggacga tggacgagtg gacaacgtgt cgcttaccgg ctgtcacctt ttttttcggg
     3421 aaacgctgac ggcaatgagg cactggctat atcccgcagg gcacccggca atgcgacagg
     3481 ttataaattt tgaattcagt ctgaaaaatg acggcttaca ggatccgcga ccagtcacca
     3541 cccaaaagct caatgatgat ggttgccgcc agtaacaggt cctttaaccc gcaggctgca
     3601 atagtcctgg atgatgagtg agcctgtagc agccctgatt agtggaagcg aagctgaaaa
     3661 atgggcagca aaagccgtat ttctggcaaa tagcgccgcc acggattaaa gtagtcatgt
     3721 gtaatacata tgactacaaa ggggctttta tgtcatcagc aacatccatc aggctcgatg
     3781 aagaacttaa ggaccggctg aaaacgctgg ccgatgaccg gcaccgctca gcgcacgcgc
     3841 taatgctgga ggcaatcacc gaatacatcg accgggaaga gaagcgcagc cagtacctgc
     3901 gggacggaca ggccgcatgg cagcactatc aggaaacggg tctgcacctt actgcggaag
     3961 aagcggaagc ctggataagt acctggggaa ctgagaacga gcaggatgct ccgccatgcc
     4021 acaggtaacg atttccgcgc tggcacagcg cgatttacaa cgccttcagg actttctcaa
     4081 aaccaaaaat cggctggcag ccagaaaagg aggtgaggtg atcgtccggg ctatccagca
     4141 actgaaaaca ttgccagaca ttggccgccc ggtgccgttt ctgccgctgg aatataagga
     4201 actggtgatc gggtttggcg acagtggcta tgtgatgctc taccgccacg accgggaaat
     4261 ggaccagatt gtgatcgtca ccgtcaggca tcagaaagaa tccgggtatc cgggggcaga
     4321 cagcctctaa taaccgatga cgggggcgca attcattgcg tggccgtcgc cggtttgaca
     4381 agcgcagcgc gtcagaaaac cacatcctga acggaaaaac gggtaaaatt cagcgataca
     4441 tttaccgtga gaaattacac acctttcaca cccgataaaa tcaggggtat acagtggcga
     4501 aaacattgct caacctttca caggcagcac aagccgcggg cattaccaga agaacgctat
     4561 acaatcatgt aaaacaggga aaggttaccg tgtcacgtga cgggaaaaac aaccctgtcg
     4621 tggatgtgtc agaactgatc cgtgtatacg gaaatgtgaa tatccctgag aaacaaattc
     4681 ccggcatttc acaccgtgaa aatacacaaa aaaatttccc acaggaacag cttctggcaa
     4741 tgcaaaagga actggccgat ctccggcagg ctgtcaccct gatgctggaa gataaaacat
     4801 cccgggaaga agaacgccgc cagcacgatg atgagcgccg gaaactccag gccgaagtgg
     4861 acagattgac aacagaactg acacagaaga aaaaacgctt ctggtccggc tggtttagct
     4921 gagaacttac cgaagggaat ggagaacatg acgaagcagg aaaaaactgc cctcaatatg
     4981 gctaaattca ttaaggatca gtcgctgctg ttgctggaaa agctgaacga gctggacatg
     5041 gataccccgg ccgacatgtg cgaacggctt cacgaggatg cggaaacact ctggcaggtg
     5101 atgcgggata agctcgggga agaataacag caccggccat gccggtgctg gcgttatttt
     5161 ttgccgaacg gccaccaccg gcgggcgggt ttgtcctccg gcagttcagg caccggcacg
     5221 ttaaaatagc ggctgttata ttcccgcatc agctccgctt tcagcttctc gttcaccgcg
     5281 ctttccccgt gccgctcgct gaccacctca atcatcgcgt taatctgctc cggtgagcac
     5341 ttcccggcat caaccacctg cacaccgttc tcgatggcga accgctccac ctttcgatag
     5401 aggtaatcac acagcttcat tcctgtgggt ttgtcccgtt cccattgcgg attttcaccg
     5461 gtgaaaaagt acgtggcttt ttcgtcgctg ttgagccgct cgtcatacat cccgatggat
     5521 ttcatcatcg ccagatgctg ggcccggtcg acaaaacaga aaacatacgc cccggtctgc
     5581 caggcgagca ccaccatgtc ttccagatgc tcgctggttg gcataatgca gcgctcgccg
     5641 tctttgtctt ccacgtcctc aataaatacc atgtacagcg ccataatccc tcactccgtt
     5701 gtcataaatt taaagcacgc gaacagcctc tcccggtcca gctccaccgt tttgcgggcc
     5761 tcgccggtct gtggattcac gatttcatcc accgccagaa tcgcccgctg tacccggaca
     5821 agcgtcggcc ggtcagggca gatgtaccag atttcatcga tgccgttcgc ccggcggttg
     5881 aacagatacc cggacaccac ttcctgatac cgcctgcggc tcttgacggt gcgctctacc
     5941 tcaaatgcca ccgttctgcc gtccagctcc gccagcgcgt ccggcacttt catccctttc
     6001 agggccatac ggtgcaggta ccgccagccg gtggcccccc ggccctccat ggagaggcgc
     6061 agggactgca cggccagccg gtgcggcagc tgcgccgccg acacccttga cggctgaaac
     6121 ggaataacgt ccgtcagcgg ctcgtcctcg tcgaacgaga acgccacccc gtgcggcgtc
     6181 agcccccaca cgctgacctg acggccgtca accgggtatt tcgcccggac cagcagccct
     6241 ttcgccacca gactgtccag gaagcggtaa accggctgta ccgccgcaat ccccagccgt
     6301 cgcgcaacca gctccgccgt ggaacacagc tcttcccgca gccagcgcag ggtcagccgt
     6361 gttttctccg cgttccgcgc ctgccgcttc gcgtggctga cgttgtactg ctccagtgtc
     6421 atcagtaaag ccccgcaaaa tcatcatccg cttttggtcc gccgccgtcg gccggaggca
     6481 ggtactccgc cagcgggtcc gttttcgcca gcaccgggaa ggttttcagc gaaatttccc
     6541 ggcgctcaac ccgcagcggg ctgctgaacg ccagccgcgc cacgcccagc ccggtcatca
     6601 ccgcgaaccc gtcaggcagg tgttgcaggg tgttcacgtc aaacagcggc cgcgcctcct
     6661 tctggatgaa gctgtcgccg ctgatgtgct ccacgttccc cgcctcccgg ctggcccggc
     6721 ggcgctccac gtccacccgg atctccccgg tctggcccgc cgcccactgc gcggactcct
     6781 ggctggccgc gcgatagaac cagcggatcg gcgtgttatc cagtaccgtg gttttcacaa
     6841 aatcagcggg cagatcctgc ccgcactgac cgaagtcgcc cagcgactgg tgcgcgagca
     6901 gcaggttgca gtcccggctg cgcagcgtcc ccagcgcgtt cagcacgtac ttcgacagca
     6961 gatatttaat ctcatcgagc atgatgctcg cgtgcggcca cctccggaac tcatcccgcg
     7021 caatgatgat ttgcgcgcag cgggcaaaca gcatcttctg cacccggatc accgcctcat
     7081 cgtccatcga gccgatgacg tacaggcagc cgccgccgtt gattatcccc gccacatcga
     7141 taccgctgtc cgtctgcagg acgctcaggt cggccacgtt ctccagcttg gtgatcagcc
     7201 ccttcacgtc ctttttcagg gcctccggca acgcgtacgc cgccgccagc acctgctgaa
     7261 tgtttgcccc ctgcgggaac tgctccgcaa tcagcttcgc cgctttctgc tcgctgatgc
     7321 ggtagtggtc cgccacgtcg ccggtctcac tcaggttaaa cccggcaacc agcagattct
     7381 tcagggcgta ctggtcgaga tcgcggaaca gattcagctg cgccggtttc cccggccgca
     7441 gatccagcag ggtgaagtcc gggcagtgcg cccggaagac gtgcggcgcc cacgcgtccc
     7501 ctttggggtc gaagtacacc acagcgtccc cgaaatcgcg tacgcagcga atgagcgcgt
     7561 tggtggccat cgcgctcttg ccgctcccgg gcaggccgag gatctgaata ttggtcttgc
     7621 gccaggtatc acgagggacc agcaccggct ggccctgctc atccttaccc agaaacaccg
     7681 cctgctgttt ccgggcctgc tccgtggaaa aatcggccag cgcgttaacc ggtaccggct
     7741 ccccgaaatg caggtggcgg acgtctgtca tttctccggt ccgctggctg ctgcgccggc
     7801 tctcctgatt cagtttcgcg ttcaccgcct cgtcagcggc cgcaaaatga ccatcaagca
     7861 gcaggaacgc cagcagccgc gccagaagga cgccggcgac aagccctccg gcacaccagg
     7921 ctatcccgaa caggttagcc ggctgcagca ggtaataatc tgcgttcgca tagaggtccg
     7981 ggcgcaggac ggtttctccc cagctctcga taaacacggc gacaaccagc ccccccagaa
     8041 gggccagacc ggcagcgccg gtcagccagt acgcgtagtt ccagacatgt cgccgtgccc
     8101 tgaccatccg cccgggtgaa tccagcgagg ccagcaccga aaatggcaca gtccggttat
     8161 tatcgcgctt atagcgatcg gcatactccc gccgcgcctt atggctcagc cccacaaaac
     8221 gcattcggcc ggcccagagt tcagccagac cgaaaaacag gccgataacc agaaaaaaac
     8281 cggccagact gtaatactcc cggtacagcc agctgaccca cgggtaaatc tgccgggcca
     8341 gctccgggat atgccactgc caggcaagcg aatccgtatt aaacatgcaa tctctctcct
     8401 tcagacgacg ggtttaccgg cgtgatgcgc agcccccgct gcacaagccg cggacatgcc
     8461 ccaaggcatg gcggcggcgg tcacgtccgt ggtgattgcg ttcagctttc accgcggagg
     8521 ccagcctctt tccgccccat gcggaaaagg cgcagccccg gaggaacaac gagcacgccc
     8581 cagcgtgtga gaggaaaagg cggcacccca gtgcggtctt ttccggtgtt ccggcccccg
     8641 tcagagttcg ccatagcgag cggctgacca cccgcaaaat caggagcagc tttgctgctt
     8701 cgtcaggtgc gtccagcacc gtggattttt gtgggtggtc aggcgtgagc ctgacggggg
     8761 ccgggtgaat ttctggcgtg gccggcgtcc agccggacag agccagaaag aggggaaaag
     8821 accgtgcgtc ggtggcgtcc agccagcgta agcaaggtct tttcccctga agtcagccac
     8881 gccccagcgg ggatgacttg catttctccg caaggagaaa aaagcaacag agataggtta
     8941 acgtaaaatg ttcagcgaaa aaccgacatg gttcacctat cctgataatt gatcgtcagg
     9001 gaatagaaag acttaatcag gggacaatat cccacatcag cgcgtccggt gaccgtcttg
     9061 caggtgccgg ggaaattgcg cctcctcgct cactcggtcg ctacgctccg ggcgtgagac
     9121 tgcggcgggc gctgcggaca catacaaagt tacccacaga ttccgtggat aagcagggga
     9181 ctaacatgtg aggcaaaacg gcagggccgc gccggtggcg tttttccata ggctccgccc
     9241 tcctgccaga gttcacataa acagacgctt tttcggtgca tctgtgggag ccgtgaggct
     9301 caaccatgaa tctgacagta cgggcgaaac ccgacaggac ttaaagatcc ccaccgtttc
     9361 cggtaggtcg ctccctcttg cgctctcctg ttccgaccct gccgtttacc ggatacctgt
     9421 ccgccgttct cccttgcggg aagtgtggcg ctttctcata gctcacacac tggtatctcg
     9481 gctcggtgta ggtcgttcgc tccaagctgg gctgtaagca agaactcccc gttcagcccg
     9541 actgctgcgc cttatccggt aactgttcac ttgagtccaa cccgggaaag cacgcaaaac
     9601 gccactggca gcagccattg gtaactggga gttcgcagag gatttgttta gctaaacacg
     9661 cggttgctct tgaagtgtgc gccaaagtcc ggctacactg gaaggacaga tttggttgct
     9721 gtgctctgcg aaagccagtt accacggtta agcagttccc caactgactt aaccttcgat
     9781 caaaccacct ccccaggtgg ttttttcgtt tacagggcaa aagattacgc gcagaaaaaa
     9841 aggatctcaa gaagatcctt tgatcttttc tactgaaccg cgatccccgt cagttcagag
     9901 gaggacgatg gtgccaccgt tccgccctga acatcccgga tataatttgc ctgacatgcg
     9961 gccagaagaa agccagcaac aatcaatata aaaagaaaaa tagtctttac ctttttcata
    10021 tactttccct gtatttaaag actccggcat tcatccctga acaccggaag tcagtatttc
    10081 aaatcatcct tgataatttt ttgttaccag ttaccgtccc gataatcaaa agctatataa
    10141 caattatcca catcaggttt tatttgattt ttaaaaaatg gctgtagtaa aggaacccat
    10201 tctttcttca aatcaaactc accattatta atattatcct ttaaaggcat tcccaagctt
    10261 tcaatgacag aaccatcgtc accaaagtct ttagaatact cttccccttt aaactcttca
    10321 gttttcttat caaaccatgt caaacgtaat ttaagcccca tatttacctc tcaaagatat
    10381 tttttaatgt tccgttttgg atctggccct ttaacctgct tacccgtttt gggatcaaat
    10441 gcgcctaggt gttcgccatc actggcacga tacccttcaa gctcaccgtg ctgggaatcc
    10501 cattcataaa ttttacgttt tttatcgcca taccatcggg cccgcttacc accaccacct
    10561 tgtttaggtg ttttaggatc acctcgcttt aactctccaa gtccctttat gtcttctgtt
    10621 ttaggtactg gatgataatc atggccgtag tctttagtcc ccttgcgagg cttgttcttt
    10681 tcctcattta acttattttc tgcatccttc gcttttttct ccttttgttt ccggctctcc
    10741 atcgccccgg taagtgcagc cttatctctg gataaggatt cattcattgc gtttaaagat
    10801 gcctgagccg cttttaattt ctcattctga ttctgaatat ccttatcaag atagcgaacc
    10861 tgatcctgcc acccggagcc atattcatga ggcgggttat aagtcacaaa atcatcttta
    10921 tttgcctctg cctcttttac ctttttatcg gcttcctgaa cagcagccct tgcaccgggg
    10981 agcgccttct ctgtcgatgc aatgcggctg tttaaggtcg caatattttt atcttcggca
    11041 tccagctcag ctttcgcttt atcatagtct cttttcagcc cctcttccgg atgagcggcg
    11101 tcccatgcct gctggcgacg cttttcttcc tcctgacgct gttttacctg tgccggggta
    11161 agaacatctg tcaccgacac ataaaccggc ttctgtccgg tctctttcgg gaaacgaata
    11221 acggcctcac gggagttacc accggctgta aaaccagccg gacgtgaatc acctttttca
    11281 gcaacaatgc cttttggcgg ggctttcgct gtcggaacac ctttcggtac gctcacctgc
    11341 agagacggga gacccggaat cgacacactg aatacccccg gacgttttgt cggtttcgca
    11401 tccaccacag ggacactcat tggccgacct gcgacaaccg caatatgctg ccgctcatcc
    11461 ttcaccacat ccacaacccg ttgtctgaca cgaaccgtcg cctggtccag tggtaaagta
    11521 cttgccggag tctccgtcac cgcatcggcc ggtaatgacg tcataatttt tgacatcatt
    11581 ttcggatcat cttttgcaat ctcagaaggc agcacaccgt agatcgcaat cccccacaga
    11641 ccaaacttaa acggcccctt cagggcagcc agcacatcag caacggcggc cgacaacgca
    11701 tcgccggaaa ctgataaagc cagtccttca gcccccggag tagccagggc aggcagaccg
    11761 aaggccatcg cagtggccga agactgtccg ccaccagagc taccgctatg attgccgctg
    11821 ttaccaccat taccaccggg tttactcccg ccgttaccaa actcccccgg attatagtta
    11881 tgaatatctc cattcggcgt atgcgtcgta ccccagcccg gcccgctgtt tggatccgta
    11941 ccaccacctg atgaagggcc acctttacca
//
