LOCUS       NZ_CP090131            10060 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain KP46 plasmid pKP46_5, complete
            sequence.
ACCESSION   NZ_CP090131
VERSION     NZ_CP090131.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN24255899
            Assembly: GCF_021397585.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Sun,Q.
  TITLE     Complete genome sequence of a Klebsiella pneumoniae isolate KP46
            carrying blaKPC-2 and blaNDM-1 genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Sun,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2021) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:56:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,581
            CDSs (total)                      :: 5,457
            Genes (coding)                    :: 5,305
            CDSs (with protein)               :: 5,305
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 54 of 152
            Pseudo Genes (incomplete)         :: 102 of 152
            Pseudo Genes (internal stop)      :: 20 of 152
            Pseudo Genes (multiple problems)  :: 20 of 152
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP090131.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP46"
                     /isolation_source="Cerebral spinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP46_5"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2017-12-14"
                     /collected_by="Qingyang Sun"
     gene            complement(join(9981..10060,1..100))
                     /locus_tag="LWR79_RS27775"
                     /old_locus_tag="LWR79_27775"
     CDS             complement(join(9981..10060,1..100))
                     /locus_tag="LWR79_RS27775"
                     /old_locus_tag="LWR79_27775"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(126..554)
                     /locus_tag="LWR79_RS27780"
                     /old_locus_tag="LWR79_27780"
     CDS             complement(126..554)
                     /locus_tag="LWR79_RS27780"
                     /old_locus_tag="LWR79_27780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(718..1032)
                     /locus_tag="LWR79_RS27785"
                     /old_locus_tag="LWR79_27785"
     CDS             complement(718..1032)
                     /locus_tag="LWR79_RS27785"
                     /old_locus_tag="LWR79_27785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1020..1298)
                     /locus_tag="LWR79_RS27790"
                     /old_locus_tag="LWR79_27790"
     CDS             complement(1020..1298)
                     /locus_tag="LWR79_RS27790"
                     /old_locus_tag="LWR79_27790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(1473..1838)
                     /locus_tag="LWR79_RS27795"
                     /old_locus_tag="LWR79_27795"
     CDS             complement(1473..1838)
                     /locus_tag="LWR79_RS27795"
                     /old_locus_tag="LWR79_27795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(1835..2206)
                     /gene="tolA"
                     /locus_tag="LWR79_RS27800"
                     /old_locus_tag="LWR79_27800"
     CDS             complement(1835..2206)
                     /gene="tolA"
                     /locus_tag="LWR79_RS27800"
                     /old_locus_tag="LWR79_27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(2480..2725)
                     /locus_tag="LWR79_RS27805"
                     /old_locus_tag="LWR79_27805"
     CDS             complement(2480..2725)
                     /locus_tag="LWR79_RS27805"
                     /old_locus_tag="LWR79_27805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            3052..4737
                     /locus_tag="LWR79_RS27810"
                     /old_locus_tag="LWR79_27810"
     CDS             3052..4737
                     /locus_tag="LWR79_RS27810"
                     /old_locus_tag="LWR79_27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4747..5004
                     /locus_tag="LWR79_RS27815"
                     /old_locus_tag="LWR79_27815"
     CDS             4747..5004
                     /locus_tag="LWR79_RS27815"
                     /old_locus_tag="LWR79_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            5089..5238
                     /locus_tag="LWR79_RS27820"
                     /old_locus_tag="LWR79_27820"
     CDS             5089..5238
                     /locus_tag="LWR79_RS27820"
                     /old_locus_tag="LWR79_27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            6722..8686
                     /locus_tag="LWR79_RS27825"
                     /old_locus_tag="LWR79_27825"
     CDS             6722..8686
                     /locus_tag="LWR79_RS27825"
                     /old_locus_tag="LWR79_27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            8686..9417
                     /gene="mobC"
                     /locus_tag="LWR79_RS27830"
                     /old_locus_tag="LWR79_27830"
     CDS             8686..9417
                     /gene="mobC"
                     /locus_tag="LWR79_RS27830"
                     /old_locus_tag="LWR79_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            9424..9954
                     /locus_tag="LWR79_RS27835"
                     /old_locus_tag="LWR79_27835"
     CDS             9424..9954
                     /locus_tag="LWR79_RS27835"
                     /old_locus_tag="LWR79_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..9737
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5342..5920)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8687..9414
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gggtatccat gtccagctcg ttcagctttt ccagcaacag cagcgactga tccttaatga
       61 atttagccat attgagggca gttttttcct gcttcgtcat gttctccatt cccttcggta
      121 agttctcagc taaaccagcc ggaccagaag cgttttttct tctgtgtcag ttctgttgtc
      181 aatctgtcca cttcggcctg gagtttccgg cgctcatcat cgtgctggcg gcgttcttct
      241 tcccgggatg ttttatcttc cagcatcagg gtgacagcct gccggagatc ggccagttcc
      301 ttttgcattg ccagaagctg ttcctgtggg aaattttttt gtgtattttc acggtgtgaa
      361 atgccgggaa tttgtttctc agggatattc acatttccgt atacacggat cagttctgac
      421 acatccacga cagggttgtt tttcccgtca cgtgacacgg taacctttcc ctgttttaca
      481 tgattgtata gcgttcttct ggtaatgccc gcggcttgtg ctgcctgtga aaggttgagc
      541 aatgttttcg ccactgtata cccctgattt tatcgggtgt gaaaggtgtg taatttctca
      601 cggtaaatgt atcgctgaat tttacccgtt tttccgttca ggatgtggtt ttctgacgcg
      661 ctgcgcttgt caaaccggcg acggccacgc aatgaattgc gcccccgtca tcggttatta
      721 gaggctgtct gcccccggat acccggattc tttctgatgc ctgacggtga cgatcacaat
      781 ctggtccatt tcccggtcgt ggcggtagag catcacatag ccactgtcgc caaacccgat
      841 caccagttcc ttatattcca gcggcagaaa cggcaccggg cggccaatgt ctggcaatgt
      901 tttcagttgc tggatagccc ggacgatcac ctcacctcct tttctggctg ccagccgatt
      961 tttggttttg agaaagtcct gaaggcgttg taaatcgcgc tgtgccagcg cggaaatcgt
     1021 tacctgtggc atggcggagc atcctgctcg ttctcagttc cccaggtact tatccaggct
     1081 tccgcttctt ccgcagtaag gtgcagaccc gtttcctgat agtgctgcca tgcggcctgt
     1141 ccgtcccgca ggtactggct gcgcttctct tcccggtcga tgtattcggt gattgcctcc
     1201 agcattagcg cgtgcgctga gcggtgccgg tcatcggcca gcgttttcag ccggtcctta
     1261 agttcttcat cgagcctgat ggatgttgct gatgacataa aagccccttt gtagtcatat
     1321 gtattacaca tgactacttt aatccgtggc ggcgctattt gccagaaata cggcttttgc
     1381 tgcccatttt tcagcttcgc ttccactaat cagggctgct acaggctcac tcatcatcca
     1441 ggactattgc agcctgcggg ttaaaggacc tgttactggc ggcaaccatc atcattgagc
     1501 ttttgggtgg tgactggtcg cggatcctgt aagccgtcat ttttcagact gaattcaaaa
     1561 tttataacct gtcgcattgc cgggtgccct gcgggatata gccagtgcct cattgccgtc
     1621 agcgtttccc gaaaaaaaag gtgacagccg gtaagcgaca cgttgtccac tcgtccatcg
     1681 tccagaatat caaacgtggc ggtcacctga ccttcgatgc gttttgctgc ggcctgtacc
     1741 gggtaggttg gctgcagtgt ggttacgggt tttagtggta tcgcatgtac cggagtgagg
     1801 aacatggccg ccagcactgt cagagtaaaa gctttcatgg tctgaaatcc agaggggcgt
     1861 ttttgaatat ttgccaggta ttttcatccg gtgccggcgg aattttggcg tgggtaacgg
     1921 ctgatattgc tgctttacag agtttcggat ctcctccttc agcaatggcg ctgttcagag
     1981 tgccatcacg ttgtatggat atccgcacgg tgcacatttt tcccgcatag gcctctggtt
     2041 caaacatctg catttcaaca gcggtcttga tggccgcagc gtagctactg attttagatg
     2101 acttatccag agtataaatt ttcggcggct gggtggtgga atgcttacag gccgtcagga
     2161 gtaaggggct gcacaatgcc agcaggataa agggtattaa acgcatcggg ttgatgacct
     2221 gtcagtaaaa aatgttacat gtatatttgt cactctttac ggccacgttc atttacataa
     2281 acacggctta tcgtatcgtt tttacaccaa acggcccgcc atgcagttgc gtgcataggc
     2341 atgcagcggg ttacaattta ccgggcgcgt ttccggcggt tttccgggtg gtttgttgcc
     2401 tgttttatcc cgtagccgcc ggaaacgccc tcagaccgtc tgagcggtgc gggcaatgcg
     2461 acgttatggt aaatacccct taccggcggc gctgtggctg ccaggtgaag gtggtttccg
     2521 tagcctcgta tttctcccga cgcatttcct gcgcccgcct ggcttcacgt tcgtcctgaa
     2581 tggcacgaag caccctttcc cgcacctgtt gttttaccgc ctttggcagc gttttgttcc
     2641 ctttcaggcc catggccagt gcctgagcga caaaggcgtc cgccaggcgc ttttcttcag
     2701 cgggagtcag tttcaggtgc gtcatagtgg cctccgggcg tctcaggttt cccgaacagt
     2761 ttaaccgcag gtcctctcct catgccagtt agctttcaga tgtgtctata ccatctgaaa
     2821 tgtaagtgtg atgaatgtct taaataataa aatttgttgc catgagaaat tttcgggaaa
     2881 agacgcgatc tgcttacagg tggctccggg cgcttttttg tgcctgaaga tgagaactaa
     2941 tctcatatat tgacacctga aaacgggagg agtaaggtaa taattatact gtgtatatgt
     3001 acagtatgtt ggctttaatt tattgtttta aatgtcaaag aggaaaacgg tatgagtggt
     3061 ggagacggtc gaggtccggg taattcaggt ctgggacata atggtggtca ggccagtggg
     3121 aatgtgaacg gtacgtctgg taaaggtggc ccttcatcag gtggtggtac ggatccaaac
     3181 agcgggccgg gctggggtac gacgcatacg ccgaatggag atattcataa ctataatccg
     3241 ggggagtttg gtaacggcgg gagtaaaccc ggtggtaatg gtggtaacag cggcaatcat
     3301 agcggtagct ctggtggcgg acagtcttcg gccactgcga tggccttcgg tctgcctgcc
     3361 ctggctactc cgggggctga aggactggct ttatcagttt ccggcgatgc gttgtcggcc
     3421 gccgttgctg atgtgctggc tgccctgaag gggccgttta agtttggtct gtgggggatt
     3481 gcgatctacg gtgtgctgcc ttctgagatt gcaaaagatg atccgaaaat gatgtcaaaa
     3541 attatgacgt cattaccggc cgatgcggtg acggagactc cggcaagtac tttaccactg
     3601 gaccaggcga cggttcgtgt cagacaacgg gttgtggatg tggtgaagga tgagcggcag
     3661 catattgcgg ttgtcgcagg tcggccaatg agtgtccctg tggtggatgc gaaaccgaca
     3721 aaacgtccgg gggtattcag tgtgtcgatt ccgggtctcc cgtctctgca ggtgagcgta
     3781 ccgaaaggtg ttccgacagc gaaagccccg ccaaaaggca ttgttgctga aaaaggtgat
     3841 tcacgtccgg ctggttttac agccggtggt aactcccgtg aggccgttat tcgtttcccg
     3901 aaagagaccg gacagaagcc ggtttatgtg tcggtgacag atgttcttac cccggcacag
     3961 gtaaaacagc gtcaggagga agaaaagcgt cgccagcagg catgggacgc cgctcatccg
     4021 gaagaggggc tgaaaagaga ctatgataaa gcgaaagctg agctggatgc cgaagataaa
     4081 aatattgcga ccttaaacag ccgcattgca tcgacagaga aggcgctccc cggtgcaagg
     4141 gctgctgttc aggaagccga taaaaaggta aaagaggcag aggcaaataa agatgatttt
     4201 gtgacttata acccgcctca tgaatatggc tccgggtggc aggatcaggt tcgctatctt
     4261 gataaggata ttcagaatca gaatgagaaa ttaaaagcgg ctcaggcatc tttaaacgca
     4321 atgaatgaat ccttatccag agataaggct gcacttaccg gggcgatgga gagccggaaa
     4381 caaaaggaga aaaaagcgaa ggatgcagaa aataagttaa atgaggaaaa gaacaagcct
     4441 cgcaagggga ctaaagacta cggccatgat tatcatccag tacctaaaac agaagacata
     4501 aagggacttg gagagttaaa gcgaggtgat cctaaaacac ctaaacaagg tggtggtggt
     4561 aagcgggccc gatggtatgg cgataaaaaa cgtaaaattt atgaatggga ttcccagcac
     4621 ggtgagcttg aagggtatcg tgccagtgat ggcgaacacc taggcgcatt tgatcccaaa
     4681 acgggtaagc aggttaaagg gccagatcca aaacggaaca ttaaaaaata tctttgagag
     4741 gtaaatatgg ggcttaaatt acgtttgaca tggtttgata agaaaactga agagtttaaa
     4801 ggggaagagt attctaaaga ctttggtgac gatggttctg tcattgaaag cttgggaatg
     4861 cctttaaagg ataatattaa taatggtgag tttgatttga agaaagaatg ggttccttta
     4921 ctacagccat tttttaaaaa tcaaataaaa cctgatgtgg ataattgtta tatagctttt
     4981 gattatcggg acggtaactg gtaacaaaaa attatcaagg atgatttgaa atactgactt
     5041 ccggtgttca gggatgaatg ccggagtctt taaatacagg gaaagtatat gaaaaaggta
     5101 aagactattt ttctttttat attgattgtt gctggctttc ttctggccgc atgtcaggca
     5161 aattatatcc gggatgttca gggcggaacg gtggcaccat cgtcctcctc tgaactgacg
     5221 gggatcgcgg ttcagtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5281 taatcttttg ccctgtaaac gaaaaaacca cctggggagg tggtttgatc gaaggttaag
     5341 tcagttgggg aactgcttaa ccgtggtaac tggctttcgc agagcacagc aaccaaatct
     5401 gtccttccag tgtagccgga ctttggcgca cacttcaaga gcaaccgcgt gtttagctaa
     5461 acaaatcctc tgcgaactcc cagttaccaa tggctgctgc cagtggcgtt ttgcgtgctt
     5521 tcccgggttg gactcaagtg aacagttacc ggataaggcg cagcagtcgg gctgaacggg
     5581 gagttcttgc ttacagccca gcttggagcg aacgacctac accgagccga gataccagtg
     5641 tgtgagctat gagaaagcgc cacacttccc gcaagggaga acggcggaca ggtatccggt
     5701 aaacggcagg gtcggaacag gagagcgcaa gagggagcga cctaccggaa acggtgggga
     5761 tctttaagtc ctgtcgggtt tcgcccgtac tgtcagattc atggttgagc ctcacggctc
     5821 ccacagatgc accgaaaaag cgtctgttta tgtgaactct ggcaggaggg cggagcctat
     5881 ggaaaaacgc caccggcgcg gccctgccgt tttgcctcac atgttagtcc cctgcttatc
     5941 cacggaatct gtgggtaact ttgtatgtgt ccgcagcgcc cgccgcagtc tcacgcccgg
     6001 agcgtagcga ccgagtgagc gaggaggcgc aatttccccg gcacctgcaa gacggtcacc
     6061 ggacgcgctg atgtgggata ttgtcccctg attaagtctt tctattccct gacgatcaat
     6121 tatcaggata ggtgaaccat gtcggttttt cgctgaacat tttacgttaa cctatctctg
     6181 ttgctttttt ctccttgcgg agaaatgcaa gtcatccccg ctggggcgtg gctgacttca
     6241 ggggaaaaga ccttgcttac gctggctgga cgccaccgac gcacggtctt ttcccctctt
     6301 tctggctctg tccggctgga cgccggccac gccagaaatt cacccggccc ccgtcaggct
     6361 cacgcctgac cacccacaaa aatccacggt gctggacgca cctgacgaag cagcaaagct
     6421 gctcctgatt ttgcgggtgg tcagccgctc gctatggcga actctgacgg gggccggaac
     6481 accggaaaag accgcactgg ggtgccgcct tttcctctca cacgctgggg cgtgctcgtt
     6541 gttcctccgg ggctgcgcct tttccgcatg gggcggaaag aggctggcct ccgcggtgaa
     6601 agctgaacgc aatcaccacg gacgtgaccg ccgccgccat gccttggggc atgtccgcgg
     6661 cttgtgcagc gggggctgcg catcacgccg gtaaacccgt cgtctgaagg agagagattg
     6721 catgtttaat acggattcgc ttgcctggca gtggcatatc ccggagctgg cccggcagat
     6781 ttacccgtgg gtcagctggc tgtaccggga gtattacagt ctggccggtt tttttctggt
     6841 tatcggcctg tttttcggtc tggctgaact ctgggccggc cgaatgcgtt ttgtggggct
     6901 gagccataag gcgcggcggg agtatgccga tcgctataag cgcgataata accggactgt
     6961 gccattttcg gtgctggcct cgctggattc acccgggcgg atggtcaggg cacggcgaca
     7021 tgtctggaac tacgcgtact ggctgaccgg cgctgccggt ctggcccttc tgggggggct
     7081 ggttgtcgcc gtgtttatcg agagctgggg agaaaccgtc ctgcgcccgg acctctatgc
     7141 gaacgcagat tattacctgc tgcagccggc taacctgttc gggatagcct ggtgtgccgg
     7201 agggcttgtc gccggcgtcc ttctggcgcg gctgctggcg ttcctgctgc ttgatggtca
     7261 ttttgcggcc gctgacgagg cggtgaacgc gaaactgaat caggagagcc ggcgcagcag
     7321 ccagcggacc ggagaaatga cagacgtccg ccacctgcat ttcggggagc cggtaccggt
     7381 taacgcgctg gccgattttt ccacggagca ggcccggaaa cagcaggcgg tgtttctggg
     7441 taaggatgag cagggccagc cggtgctggt ccctcgtgat acctggcgca agaccaatat
     7501 tcagatcctc ggcctgcccg ggagcggcaa gagcgcgatg gccaccaacg cgctcattcg
     7561 ctgcgtacgc gatttcgggg acgctgtggt gtacttcgac cccaaagggg acgcgtgggc
     7621 gccgcacgtc ttccgggcgc actgcccgga cttcaccctg ctggatctgc ggccggggaa
     7681 accggcgcag ctgaatctgt tccgcgatct cgaccagtac gccctgaaga atctgctggt
     7741 tgccgggttt aacctgagtg agaccggcga cgtggcggac cactaccgca tcagcgagca
     7801 gaaagcggcg aagctgattg cggagcagtt cccgcagggg gcaaacattc agcaggtgct
     7861 ggcggcggcg tacgcgttgc cggaggccct gaaaaaggac gtgaaggggc tgatcaccaa
     7921 gctggagaac gtggccgacc tgagcgtcct gcagacggac agcggtatcg atgtggcggg
     7981 gataatcaac ggcggcggct gcctgtacgt catcggctcg atggacgatg aggcggtgat
     8041 ccgggtgcag aagatgctgt ttgcccgctg cgcgcaaatc atcattgcgc gggatgagtt
     8101 ccggaggtgg ccgcacgcga gcatcatgct cgatgagatt aaatatctgc tgtcgaagta
     8161 cgtgctgaac gcgctgggga cgctgcgcag ccgggactgc aacctgctgc tcgcgcacca
     8221 gtcgctgggc gacttcggtc agtgcgggca ggatctgccc gctgattttg tgaaaaccac
     8281 ggtactggat aacacgccga tccgctggtt ctatcgcgcg gccagccagg agtccgcgca
     8341 gtgggcggcg ggccagaccg gggagatccg ggtggacgtg gagcgccgcc gggccagccg
     8401 ggaggcgggg aacgtggagc acatcagcgg cgacagcttc atccagaagg aggcgcggcc
     8461 gctgtttgac gtgaacaccc tgcaacacct gcctgacggg ttcgcggtga tgaccgggct
     8521 gggcgtggcg cggctggcgt tcagcagccc gctgcgggtt gagcgccggg aaatttcgct
     8581 gaaaaccttc ccggtgctgg cgaaaacgga cccgctggcg gagtacctgc ctccggccga
     8641 cggcggcgga ccaaaagcgg atgatgattt tgcggggctt tactgatgac actggagcag
     8701 tacaacgtca gccacgcgaa gcggcaggcg cggaacgcgg agaaaacacg gctgaccctg
     8761 cgctggctgc gggaagagct gtgttccacg gcggagctgg ttgcgcgacg gctggggatt
     8821 gcggcggtac agccggttta ccgcttcctg gacagtctgg tggcgaaagg gctgctggtc
     8881 cgggcgaaat acccggttga cggccgtcag gtcagcgtgt gggggctgac gccgcacggg
     8941 gtggcgttct cgttcgacga ggacgagccg ctgacggacg ttattccgtt tcagccgtca
     9001 agggtgtcgg cggcgcagct gccgcaccgg ctggccgtgc agtccctgcg cctctccatg
     9061 gagggccggg gggccaccgg ctggcggtac ctgcaccgta tggccctgaa agggatgaaa
     9121 gtgccggacg cgctggcgga gctggacggc agaacggtgg catttgaggt agagcgcacc
     9181 gtcaagagcc gcaggcggta tcaggaagtg gtgtccgggt atctgttcaa ccgccgggcg
     9241 aacggcatcg atgaaatctg gtacatctgc cctgaccggc cgacgcttgt ccgggtacag
     9301 cgggcgattc tggcggtgga tgaaatcgtg aatccacaga ccggcgaggc ccgcaaaacg
     9361 gtggagctgg accgggagag gctgttcgcg tgctttaaat ttatgacaac ggagtgaggg
     9421 attatggcgc tgtacatggt atttattgag gacgtggaag acaaagacgg cgagcgctgc
     9481 attatgccaa ccagcgagca tctggaagac atggtggtgc tcgcctggca gaccggggcg
     9541 tatgttttct gttttgtcga ccgggcccag catctggcga tgatgaaatc catcgggatg
     9601 tatgacgagc ggctcaacag cgacgaaaaa gccacgtact ttttcaccgg tgaaaatccg
     9661 caatgggaac gggacaaacc cacaggaatg aagctgtgtg attacctcta tcgaaaggtg
     9721 gagcggttcg ccatcgagaa cggtgtgcag gtggttgatg ccgggaagtg ctcaccggag
     9781 cagattaacg cgatgattga ggtggtcagc gagcggcacg gggaaagcgc ggtgaacgag
     9841 aagctgaaag cggagctgat gcgggaatat aacagccgct attttaacgt gccggtgcct
     9901 gaactgccgg aggacaaacc cgcccgccgg tggtggccgt tcggcaaaaa ataacgccag
     9961 caccggcatg gccggtgctg ttattcttcc ccgagcttat cccgcatcac ctgccagagt
    10021 gtttccgcat cctcgtgaag ccgttcgcac atgtcggccg
//
