LOCUS       NZ_CP089992            11970 bp    DNA     circular CON 02-DEC-2023
DEFINITION  Klebsiella pneumoniae strain LZKP00003 plasmid pZR1, complete
            sequence.
ACCESSION   NZ_CP089992
VERSION     NZ_CP089992.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23803106
            Assembly: GCF_021389995.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Zeng,Z.
  TITLE     klebsiella pneumoniae ST2407, complete sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Zeng,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2021) Department of Laboratory Medicine, The
            Affiliated Hospital of Southwest Medical University, 25 Taiping
            Street, Luzhou 646000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/02/2023 05:15:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,428
            CDSs (total)                      :: 5,310
            Genes (coding)                    :: 5,165
            CDSs (with protein)               :: 5,165
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 52 of 145
            Pseudo Genes (incomplete)         :: 96 of 145
            Pseudo Genes (internal stop)      :: 24 of 145
            Pseudo Genes (multiple problems)  :: 23 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP089992.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="LZKP00003"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pZR1"
                     /geo_loc_name="China:Luzhou"
                     /collection_date="2019-05-22"
     gene            join(10348..11970,1..63)
                     /locus_tag="LUR00_RS26970"
                     /old_locus_tag="LUR00_26970"
     CDS             join(10348..11970,1..63)
                     /locus_tag="LUR00_RS26970"
                     /old_locus_tag="LUR00_26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            73..330
                     /locus_tag="LUR00_RS26975"
                     /old_locus_tag="LUR00_26975"
     CDS             73..330
                     /locus_tag="LUR00_RS26975"
                     /old_locus_tag="LUR00_26975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            415..564
                     /locus_tag="LUR00_RS26980"
                     /old_locus_tag="LUR00_26980"
     CDS             415..564
                     /locus_tag="LUR00_RS26980"
                     /old_locus_tag="LUR00_26980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            2048..4012
                     /locus_tag="LUR00_RS26985"
                     /old_locus_tag="LUR00_26985"
     CDS             2048..4012
                     /locus_tag="LUR00_RS26985"
                     /old_locus_tag="LUR00_26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            4012..4743
                     /gene="mobC"
                     /locus_tag="LUR00_RS26990"
                     /old_locus_tag="LUR00_26990"
     CDS             4012..4743
                     /gene="mobC"
                     /locus_tag="LUR00_RS26990"
                     /old_locus_tag="LUR00_26990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            4750..5280
                     /locus_tag="LUR00_RS26995"
                     /old_locus_tag="LUR00_26995"
     CDS             4750..5280
                     /locus_tag="LUR00_RS26995"
                     /old_locus_tag="LUR00_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5307..5486)
                     /locus_tag="LUR00_RS27000"
                     /old_locus_tag="LUR00_27000"
     CDS             complement(5307..5486)
                     /locus_tag="LUR00_RS27000"
                     /old_locus_tag="LUR00_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5512..5940)
                     /locus_tag="LUR00_RS27005"
                     /old_locus_tag="LUR00_27005"
     CDS             complement(5512..5940)
                     /locus_tag="LUR00_RS27005"
                     /old_locus_tag="LUR00_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(6104..6418)
                     /locus_tag="LUR00_RS27010"
                     /old_locus_tag="LUR00_27010"
     CDS             complement(6104..6418)
                     /locus_tag="LUR00_RS27010"
                     /old_locus_tag="LUR00_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6406..6684)
                     /locus_tag="LUR00_RS27015"
                     /old_locus_tag="LUR00_27015"
     CDS             complement(6406..6684)
                     /locus_tag="LUR00_RS27015"
                     /old_locus_tag="LUR00_27015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(6859..7224)
                     /locus_tag="LUR00_RS27020"
                     /old_locus_tag="LUR00_27020"
     CDS             complement(6859..7224)
                     /locus_tag="LUR00_RS27020"
                     /old_locus_tag="LUR00_27020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(7221..7592)
                     /gene="tolA"
                     /locus_tag="LUR00_RS27025"
                     /old_locus_tag="LUR00_27025"
     CDS             complement(7221..7592)
                     /gene="tolA"
                     /locus_tag="LUR00_RS27025"
                     /old_locus_tag="LUR00_27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(7866..8111)
                     /locus_tag="LUR00_RS27030"
                     /old_locus_tag="LUR00_27030"
     CDS             complement(7866..8111)
                     /locus_tag="LUR00_RS27030"
                     /old_locus_tag="LUR00_27030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8756..10243
                     /gene="ltrA"
                     /locus_tag="LUR00_RS27035"
                     /old_locus_tag="LUR00_27035"
     CDS             8756..10243
                     /gene="ltrA"
                     /locus_tag="LUR00_RS27035"
                     /old_locus_tag="LUR00_27035"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     BGC             5348..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(668..1246)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4013..4740
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 cccaaaacgg gtaagcaggt taaagggcca gatccaaaac ggaacattaa aaaatatctt
       61 tgagaggtaa atatggggct taaattacgt ttgacatggt ttgataagaa aactgaagag
      121 tttaaagggg aagagtattc taaagacttt ggtgacgatg gttctgtcat tgaaagcttg
      181 ggaatgcctt taaaggataa tattaataat ggtgagtttg atttgaagaa agaatgggtt
      241 cctttactac agccattttt taaaaatcaa ataaaacctg atgtggataa ttgttatata
      301 gcttttgatt atcgggacgg taactggtaa caaaaaatta tcaaggatga tttgaaatac
      361 tgacttccgg tgttcaggga tgaatgccgg agtctttaaa tacagggaaa gtatatgaaa
      421 aaggtaaaga ctatttttct ttttatattg attgttgctg gctttcttct ggccgcatgt
      481 caggcaaatt atatccggga tgttcagggc ggaacggtgg caccatcgtc ctcctctgaa
      541 ctgacgggga tcgcggttca gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
      601 tgcgcgtaat cttttgccct gtaaacgaaa aaaccacctg gggaggtggt ttgatcgaag
      661 gttaagtcag ttggggaact gcttaaccgt ggtaactggc tttcgcagag cacagcaacc
      721 aaatctgtcc ttccagtgta gccggacttt ggcgcacact tcaagagcaa ccgcgtgttt
      781 agctaaacaa atcctctgcg aactcccagt taccaatggc tgctgccagt ggcgttttgc
      841 gtgctttccc gggttggact caagtgaaca gttaccggat aaggcgcagc agtcgggctg
      901 aacggggagt tcttgcttac agcccagctt ggagcgaacg acctacaccg agccgagata
      961 ccagtgtgtg agctatgaga aagcgccaca cttcccgcaa gggagaacgg cggacaggta
     1021 tccggtaaac ggcagggtcg gaacaggaga gcgcaagagg gagcgaccta ccggaaacgg
     1081 tggggatctt taagtcctgt cgggtttcgc ccgtactgtc agattcatgg ttgagcctca
     1141 cggctcccac agatgcaccg aaaaagcgtc tgtttatgtg aactctggca ggagggcgga
     1201 gcctatggaa aaacgccacc ggcgcggccc tgccgttttg cctcacatgt tagtcccctg
     1261 cttatccacg gaatctgtgg gtaactttgt atgtgtccgc agcgcccgcc gcagtctcac
     1321 gcccggagcg tagcgaccga gtgagcgagg aggcgcaatt tccccggcac ctgcaagacg
     1381 gtcaccggac gcgctgatgt gggatattgt cccctgatta agtctttcta ttccctgacg
     1441 atcaattatc aggataggtg aaccatgtcg gtttttcgct gaacatttta cgttaaccta
     1501 tctctgttgc ttttttctcc ttgcggagaa atgcaagtca tccccgctgg ggcgtggctg
     1561 acttcagggg aaaagacctt gcttacgctg gctggacgcc accgacgcac ggtcttttcc
     1621 cctctttctg gctctgtccg gctggacgcc ggccacgcca gaaattcacc cggcccccgt
     1681 caggctcacg cctgaccacc cacaaaaatc cacggtgctg gacgcacctg acgaagcagc
     1741 aaagctgctc ctgattttgc gggtggtcag ccgctcgcta tggcgaactc tgacgggggc
     1801 cggaacaccg gaaaagaccg cactggggtg ccgccttttc ctctcacacg ctggggcgtg
     1861 ctcgttgttc ctccggggct gcgccttttc cgcatggggc ggaaagaggc tggcctccgc
     1921 ggtgaaagct gaacgcaatc accacggacg tgaccgccgc cgccatgcct tggggcatgt
     1981 ccgcggcttg tgcagcgggg gctgcgcatc acgccggtaa acccgtcgtc tgaaggagag
     2041 agattgcatg tttaatacgg attcgcttgc ctggcagtgg catatcccgg agctggcccg
     2101 gcagatttac ccgtgggtca gctggctgta ccgggagtat tacagtctgg ccggtttttt
     2161 tctggttatc ggcctgtttt tcggtctggc tgaactctgg gccggccgaa tgcgttttgt
     2221 ggggctgagc cataaggcgc ggcgggagta tgccgatcgc tataagcgcg ataataaccg
     2281 gactgtgcca ttttcggtgc tggcctcgct ggattcaccc gggcggatgg tcagggcacg
     2341 gcgacatgtc tggaactacg cgtactggct gaccggcgct gccggtctgg cccttctggg
     2401 ggggctggtt gtcgccgtgt ttatcgagag ctggggagaa accgtcctgc gcccggacct
     2461 ctatgcgaac gcagattatt acctgctgca gccggctaac ctgttcggga tagcctggtg
     2521 tgccggaggg cttgtcgccg gcgtccttct ggcgcggctg ctggcgttcc tgctgcttga
     2581 tggtcatttt gcggccgctg acgaggcggt gaacgcgaaa ctgaatcagg agagccggcg
     2641 cagcagccag cggaccggag aaatgacaga cgtccgccac ctgcatttcg gggagccggt
     2701 accggttaac gcgctggccg atttttccac ggagcaggcc cggaaacagc aggcggtgtt
     2761 tctgggtaag gatgagcagg gccagccggt gctggtccct cgtgatacct ggcgcaagac
     2821 caatattcag atcctcggcc tgcccgggag cggcaagagc gcgatggcca ccaacgcgct
     2881 cattcgctgc gtacgcgatt tcggggacgc tgtggtgtac ttcgacccca aaggggacgc
     2941 gtgggcgccg cacgtcttcc gggcgcactg cccggacttc accctgctgg atctgcggcc
     3001 ggggaaaccg gcgcagctga atctgttccg cgatctcgac cagtacgccc tgaagaatct
     3061 gctggttgcc gggtttaacc tgagtgagac cggcgacgtg gcggaccact accgcatcag
     3121 cgagcagaaa gcggcgaagc tgattgcgga gcagttcccg cagggggcaa acattcagca
     3181 ggtgctggcg gcggcgtacg cgttgccgga ggccctgaaa aaggacgtga aggggctgat
     3241 caccaagctg gagaacgtgg ccgacctgag cgtcctgcag acggacagcg gtatcgatgt
     3301 ggcggggata atcaacggcg gcggctgcct gtacgtcatc ggctcgatgg acgatgaggc
     3361 ggtgatccgg gtgcagaaga tgctgtttgc ccgctgcgcg caaatcatca ttgcgcggga
     3421 tgagttccgg aggtggccgc acgcgagcat catgctcgat gagattaaat atctgctgtc
     3481 gaagtacgtg ctgaacgcgc tggggacgct gcgcagccgg gactgcaacc tgctgctcgc
     3541 gcaccagtcg ctgggcgact tcggtcagtg cgggcaggat ctgcccgctg attttgtgaa
     3601 aaccacggta ctggataaca cgccgatccg ctggttctat cgcgcggcca gccaggagtc
     3661 cgcgcagtgg gcggcgggcc agaccgggga gatccgggtg gacgtggagc gccgccgggc
     3721 cagccgggag gcggggaacg tggagcacat cagcggcgac agcttcatcc agaaggaggc
     3781 gcggccgctg tttgacgtga acaccctgca acacctgcct gacgggttcg cggtgatgac
     3841 cgggctgggc gtggcgcggc tggcgttcag cagcccgctg cgggttgagc gccgggaaat
     3901 ttcgctgaaa accttcccgg tgctggcgaa aacggacccg ctggcggagt acctgcctcc
     3961 ggccgacggc ggcggaccaa aagcggatga tgattttgcg gggctttact gatgacactg
     4021 gagcagtaca acgtcagcca cgcgaagcgg caggcgcgga acgcggagaa aacacggctg
     4081 accctgcgct ggctgcggga agagctgtgt tccacggcgg agctggttgc gcgacggctg
     4141 gggattgcgg cggtacagcc ggtttaccgc ttcctggaca gtctggtggc gaaagggctg
     4201 ctggtccggg cgaaataccc ggttgacggc cgtcaggtca gcgtgtgggg gctgacgccg
     4261 cacggggtgg cgttctcgtt cgacgaggac gagccgctga cggacgttat tccgtttcag
     4321 ccgtcaaggg tgtcggcggc gcagctgccg caccggctgg ccgtgcagtc cctgcgcctc
     4381 tccatggagg gccggggggc caccggctgg cggtacctgc accgtatggc cctgaaaggg
     4441 atgaaagtgc cggacgcgct ggcggagctg gacggcagaa cggtggcatt tgaggtagag
     4501 cgcaccgtca agagccgcag gcggtatcag gaagtggtgt ccgggtatct gttcaaccgc
     4561 cgggcgaacg gcatcgatga aatctggtac atctgccctg accggccgac gcttgtccgg
     4621 gtacagcggg cgattctggc ggtggatgaa atcgtgaatc cacagaccgg cgaggcccgc
     4681 aaaacggtgg agctggaccg ggagaggctg ttcgcgtgct ttaaatttat gacaacggag
     4741 tgagggatta tggcgctgta catggtattt attgaggacg tggaagacaa agacggcgag
     4801 cgctgcatta tgccaaccag cgagcatctg gaagacatgg tggtgctcgc ctggcagacc
     4861 ggggcgtatg ttttctgttt tgtcgaccgg gcccagcatc tggcgatgat gaaatccatc
     4921 gggatgtatg acgagcggct caacagcgac gaaaaagcca cgtacttttt caccggtgaa
     4981 aatccgcaat gggaacggga caaacccaca ggaatgaagc tgtgtgatta cctctatcga
     5041 aaggtggagc ggttcgccat cgagaacggt gtgcaggtgg ttgatgccgg gaagtgctca
     5101 ccggagcaga ttaacgcgat gattgaggtg gtcagcgagc ggcacgggga aagcgcggtg
     5161 aacgagaagc tgaaagcgga gctgatgcgg gaatataaca gccgctattt taacgtgccg
     5221 gtgcctgaac tgccggagga caaacccgcc cgccggtggt ggccgttcgg caaaaaataa
     5281 cgccagcacc ggcatggccg gtgctgttat tcttccccga gcttatcccg catcacctgc
     5341 cagagtgttt ccgcatcctc gtgaagccgt tcgcacatgt cggccggggt atccatgtcc
     5401 agctcgttca gcttttccag caacagcagc gactgatcct taatgaattt agccatattg
     5461 agggcagttt tttcctgctt cgtcatgttc tccattccct tcggtaagtt ctcagctaaa
     5521 ccagccggac cagaagcgtt ttttcttctg tgtcagttct gttgtcaatc tgtccacttc
     5581 ggcctggagt ttccggcgct catcatcgtg ctggcggcgt tcttcttccc gggatgtttt
     5641 atcttccagc atcagggtga cagcctgccg gagatcggcc agttcctttt gcattgccag
     5701 aagctgttcc tgtgggaaat ttttttgtgt attttcacgg tgtgaaatgc cgggaatttg
     5761 tttctcaggg atattcacat ttccgtatac acggatcagt tctgacacat ccacgacagg
     5821 gttgtttttc ccgtcacgtg acacggtaac ctttccctgt tttacatgat tgtatagcgt
     5881 tcttctggta atgcccgcgg cttgtgctgc ctgtgaaagg ttgagcaatg ttttcgccac
     5941 tgtatacccc tgattttatc gggtgtgaaa ggtgtgtaat ttctcacggt aaatgtatcg
     6001 ctgaatttta cccgtttttc cgttcaggat gtggttttct gacgcgctgc gcttgtcaaa
     6061 ccggcgacgg ccacgcaatg aattgcgccc ccgtcatcgg ttattagagg ctgtctgccc
     6121 ccggataccc ggattctttc tgatgcctga cggtgacgat cacaatctgg tccatttccc
     6181 ggtcgtggcg gtagagcatc acatagccac tgtcgccaaa cccgatcacc agttccttat
     6241 attccagcgg cagaaacggc accgggcggc caatgtctgg caatgttttc agttgctgga
     6301 tagcccggac gatcacctca cctccttttc tggctgccag ccgatttttg gttttgagaa
     6361 agtcctgaag gcgttgtaaa tcgcgctgtg ccagcgcgga aatcgttacc tgtggcatgg
     6421 cggagcatcc tgctcgttct cagttcccca ggtacttatc caggcttccg cttcttccgc
     6481 agtaaggtgc agacccgttt cctgatagtg ctgccatgcg gcctgtccgt cccgcaggta
     6541 ctggctgcgc ttctcttccc ggtcgatgta ttcggtgatt gcctccagca ttagcgcgtg
     6601 cgctgagcgg tgccggtcat cggccagcgt tttcagccgg tccttaagtt cttcatcgag
     6661 cctgatggat gttgctgatg acataaaagc ccctttgtag tcatatgtat tacacatgac
     6721 tactttaatc cgtggcggcg ctatttgcca gaaatacggc ttttgctgcc catttttcag
     6781 cttcgcttcc actaatcagg gctgctacag gctcactcat catccaggac tattgcagcc
     6841 tgcgggttaa aggacctgtt actggcggca accatcatca ttgagctttt gggtggtgac
     6901 tggtcgcgga tcctgtaagc cgtcattttt cagactgaat tcaaaattta taacctgtcg
     6961 cattgccggg tgccctgcgg gatatagcca gtgcctcatt gccgtcagcg tttcccgaaa
     7021 aaaaaggtga cagccggtaa gcgacacgtt gtccactcgt ccatcgtcca gaatatcaaa
     7081 cgtggcggtc acctgacctt cgatgcgttt tgctgcggcc tgtaccgggt aggttggctg
     7141 cagtgtggtt acgggtttta gtggtatcgc atgtaccgga gtgaggaaca tggccgccag
     7201 cactgtcaga gtaaaagctt tcatggtctg aaatccagag gggcgttttt gaatatttgc
     7261 caggtatttt catccggtgc cggcggaatt ttggcgtggg taacggctga tattgctgct
     7321 ttacagagtt tcggatctcc tccttcagca atggcgctgt tcagagtgcc atcacgttgt
     7381 atggatatcc gcacggtgca catttttccc gcataggcct ctggttcaaa catctgcatt
     7441 tcaacagcgg tcttgatggc cgcagcgtag ctactgattt tagatgactt atccagagta
     7501 taaattttcg gcggctgggt ggtggaatgc ttacaggccg tcaggagtaa ggggctgcac
     7561 aatgccagca ggataaaggg tattaaacgc atcgggttga tgacctgtca gtaaaaaatg
     7621 ttacatgtat atttgtcact ctttacggcc acgttcattt acataaacac ggcttatcgt
     7681 atcgttttta caccaaacgg cccgccatgc agttgcgtgc ataggcatgc agcgggttac
     7741 aatttaccgg gcgcgtttcc ggcggttttc cgggtggttt gttgcctgtt ttatcccgta
     7801 gccgccggaa acgccctcag accgtctgag cggtgcgggc aatgcgacgt tatggtaaat
     7861 accccttacc ggcggcgctg tggctgccag gtgaaggtgg tttccgtagc ctcgtatttc
     7921 tcccgacgca tttcctgcgc ccgcctggct tcacgttcgt cctgaatggc acgaagcacc
     7981 ctttcccgca cctgttgttt taccgccttt ggcagcgttt tgttcccttt caggcccatg
     8041 gccagtgcct gagcgacaaa ggcgtccgcc aggcgctttt cttcagcggg agtcagtttc
     8101 aggtgcgtca tagtggcctc cgggcgtctc aggtttcccg aacagtttaa ccgcaggtcc
     8161 tctcctcatg ccagttagct ttcagatgtg tctataccat ctgaaatgta agtgtgatga
     8221 atgtcttaaa taataaaatt tgttgccatg agaaattttc gggaaaagac gcgatctgct
     8281 tacaggtggc tccgggcgct tttttgtgcc tgaagatgag aactaatctc atatattgac
     8341 acctgaaaac gggaggagta aggtaataat tatactgtgt atatgtacag tgtgcgccgg
     8401 gagaccggtt agagatcaag gggtgaaagt ccccgaccat tgaaggacca gcaatccaca
     8461 aggtccccga gtcatgcgtt gcataccgcg aggtatgggg cgaagcgttg acaggggtgt
     8521 tgacaggcca gccattgagc cacgaaatgt atattaaatt accgggtgcc gacgttgtac
     8581 tgttaacgga aggcaacatc atagggtgcg atactgcgag tgccacatgg acccggcggg
     8641 gtctgagacc ctggcatgtc aatacgatct ctacgcggga accgggagat ctcccctctg
     8701 accatctgcc agtgtcggag atggcccgca ccgggaagac gaggagtcat agccggtgat
     8761 gtacggagag gagaagtcgg actcgctcat agtagcggcg aagcaggcga acaacccgaa
     8821 aggagcggag tcagtggagc gaaggagcgg ggccaagggg aacgcggaac agccacacat
     8881 gcgccggaca cagagccggg aaagcatgtc acagaggctg tcacgcgtgc gggaagctgc
     8941 gaagcagcgg aagaaagaac ggtttacagc attgttccac ctgctgacag tcgaagcact
     9001 ggaagccgca ttcctctccc tgagcaggaa agcggccgcc ggagtggatg gcatcaggtg
     9061 gatggactac gccggaaaca tgaagaacaa cataacagat ctgcaccgga ggctacatca
     9121 gggcagctac agggcgcagc ccggcaggcg tcactacatc ccaaaagcgg atggaaaaca
     9181 acgcccgctc ggcatcgcct cgctggagga caagatcgtc cagtatgcgc tggtgaaaat
     9241 cctgaacgca gtctatgaaa acgactttat ggggttctca tacgggttca gacccgggcg
     9301 aagccagcac gatgcactgg acgcactggc cacagggctg gtacgcacta acgtaaactg
     9361 ggtactggat gccgacatca gtcagttctt cgacagggtg agccacgaat ggctgatcag
     9421 gttcacagag catcggatcg gcgaccggag ggtaatcagg ctcatacgta agtggctcac
     9481 agccgggacg tcggaggagg gtcaatggcg agcaacggag gaaggcaccc cacagggtgc
     9541 ggtcatctca ccgctgctgg caaacatata cctccactac gtcttcgatc tgtgggcgca
     9601 tcagtggcga cgtcgctatg ccacaggcaa tgtggtaatg gtcagatacg ccgatgacat
     9661 cgtcatcggg ttcgacaaac gatacgatgc ccggcgcttc cgtatagcca tgcagcgcag
     9721 actgagggag ttcggactca cggttcaccc ggagaaaacc cgtctgatgg agttcggccg
     9781 cttcgctgcc gaaaaccgtg ccatcagggg aaaaggcaaa ccagaaacgt tcaacttcct
     9841 cgggttcacg cacatcagcg ggaaagatcg caacggcagg ttcatgctga tacgaaagac
     9901 ccgccgggat cggatgacgg caactctgaa agccatcaaa gacggtctgc gaaggcgctg
     9961 gcattactca atccccgaac agggaaaatg gctcaggaga gtggttcagg gatacctgaa
    10021 ctatcactcg gtaccgggca acttccccac catgcagaag ttcaggacac acgtaacaaa
    10081 cctctggcgc cgggcgctca ggcgcaggag ccagaaggat gatacgacct ggacgaaagc
    10141 aaacaaactg gcagccgcat ggctaccaag ggttcgggtt cttcatccat ggcctgtgga
    10201 gcggttcacc gccagacacc cgaggcagga gcccggtgcg taaatcgcgc acgccgggat
    10261 ctgtgcgggg ggtatccggt aacgggtatc cctaccgcga catgttggct ttaatttatt
    10321 gttttaaatg tcaaagagga aaacggtatg agtggtggag acggtcgagg tccgggtaat
    10381 tcaggtctgg gacataatgg tggtcaggcc agtgggaatg tgaacggtac gtctggtaaa
    10441 ggtggccctt catcaggtgg tggtacggat ccaaacagcg ggccgggctg gggtacgacg
    10501 catacgccga atggagatat tcataactat aatccggggg agtttggtaa cggcgggagt
    10561 aaacccggtg gtaatggtgg taacagcggc aatcatagcg gtagctctgg tggcggacag
    10621 tcttcggcca ctgcgatggc cttcggtctg cctgccctgg ctactccggg ggctgaagga
    10681 ctggctttat cagtttccgg cgatgcgttg tcggccgccg ttgctgatgt gctggctgcc
    10741 ctgaaggggc cgtttaagtt tggtctgtgg gggattgcga tctacggtgt gctgccttct
    10801 gagattgcaa aagatgatcc gaaaatgatg tcaaaaatta tgacgtcatt accggccgat
    10861 gcggtgacgg agactccggc aagtacttta ccactggacc aggcgacggt tcgtgtcaga
    10921 caacgggttg tggatgtggt gaaggatgag cggcagcata ttgcggttgt cgcaggtcgg
    10981 ccaatgagtg tccctgtggt ggatgcgaaa ccgacaaaac gtccgggggt attcagtgtg
    11041 tcgattccgg gtctcccgtc tctgcaggtg agcgtaccga aaggtgttcc gacagcgaaa
    11101 gccccgccaa aaggcattgt tgctgaaaaa ggtgattcac gtccggctgg ttttacagcc
    11161 ggtggtaact cccgtgaggc cgttattcgt ttcccgaaag agaccggaca gaagccggtt
    11221 tatgtgtcgg tgacagatgt tcttaccccg gcacaggtaa aacagcgtca ggaggaagaa
    11281 aagcgtcgcc agcaggcatg ggacgccgct catccggaag aggggctgaa aagagactat
    11341 gataaagcga aagctgagct ggatgccgaa gataaaaata ttgcgacctt aaacagccgc
    11401 attgcatcga cagagaaggc gctccccggt gcaagggctg ctgttcagga agccgataaa
    11461 aaggtaaaag aggcagaggc aaataaagat gattttgtga cttataaccc gcctcatgaa
    11521 tatggctccg ggtggcagga tcaggttcgc tatcttgata aggatattca gaatcagaat
    11581 gagaaattaa aagcggctca ggcatcttta aacgcaatga atgaatcctt atccagagat
    11641 aaggctgcac ttaccggggc gatggagagc cggaaacaaa aggagaaaaa agcgaaggat
    11701 gcagaaaata agttaaatga ggaaaagaac aagcctcgca aggggactaa agactacggc
    11761 catgattatc atccagtacc taaaacagaa gacataaagg gacttggaga gttaaagcga
    11821 ggtgatccta aaacacctaa acaaggtggt ggtggtaagc gggcccgatg gtatggcgat
    11881 aaaaaacgta aaatttatga atgggattcc cagcacggtg agcttgaagg gtatcgtgcc
    11941 agtgatggcg aacacctagg cgcatttgat
//
