LOCUS       NZ_CP089988             4097 bp    DNA     circular CON 02-DEC-2023
DEFINITION  Klebsiella pneumoniae strain LZKP00001 plasmid unnamed, complete
            sequence.
ACCESSION   NZ_CP089988
VERSION     NZ_CP089988.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23803102
            Assembly: GCF_021390015.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Zeng,Z.
  TITLE     klebsiella pneumoniae ST35, complete sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Zeng,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2021) Department of Laboratory Medicine, The
            Affiliated Hospital of Southwest Medical University, 25 Taiping
            Street, Luzhou 646000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/02/2023 05:09:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,163
            CDSs (total)                      :: 5,043
            Genes (coding)                    :: 4,959
            CDSs (with protein)               :: 4,959
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 40 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 11 of 84
            Pseudo Genes (multiple problems)  :: 10 of 84
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP089988.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="LZKP00001"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed"
                     /geo_loc_name="China:Luzhou"
                     /collection_date="2020-12-14"
     gene            530..724
                     /locus_tag="LUA09_RS25720"
                     /old_locus_tag="LUA09_25720"
     CDS             530..724
                     /locus_tag="LUA09_RS25720"
                     /old_locus_tag="LUA09_25720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_072644309.1"
                     /translation="MNKQQQIALNMARFIKSQSLTLLEKLDALDADEQATMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1714..2388)
                     /locus_tag="LUA09_RS25725"
                     /old_locus_tag="LUA09_25725"
     CDS             complement(1714..2388)
                     /locus_tag="LUA09_RS25725"
                     /old_locus_tag="LUA09_25725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_072644308.1"
                     /translation="MRTIVYVDGFNFYHGLVKRSGYKWLDLYKLFEEQLLPIRSRESQL
                     IQVKFFTAEIKAKFASRGELAHQAQQAYHRALVQIYGDNLQIIKGYYSERQECAMTFNE
                     GHPPSKTDRTTVWKLEEKQTDVRMALEMYRDAIRGEAEQLVLCTNDSDMIPPIEMIKAD
                     RPELKIGIVVPREQDTSRVISSLASLGDWSLPYVSYEALRLALLPDKVPTRRKPILKPN
                     HW"
                     /name="NYN domain-containing protein"
     gene            2736..3152
                     /locus_tag="LUA09_RS25730"
                     /old_locus_tag="LUA09_25730"
     CDS             2736..3152
                     /locus_tag="LUA09_RS25730"
                     /old_locus_tag="LUA09_25730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283459.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_057103209.1"
                     /translation="MSDQKNNLDLQKQTLESKWGKEALSMGWSAIPTSLFFLQGALNMT
                     PTTFNVLLHLIIHWWKPYDWPHPSQESIAVRMGVSSRTVQRSLQELENYGLLTKIRTSK
                     DNPKYKGRNIYDMSLLVKSLNILTPDLNAKLNEK"
                     /name="helix-turn-helix domain-containing protein"
     gene            3317..3580
                     /locus_tag="LUA09_RS25735"
                     /old_locus_tag="LUA09_25735"
     CDS             3317..3580
                     /locus_tag="LUA09_RS25735"
                     /old_locus_tag="LUA09_25735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_072644307.1"
                     /translation="MAVYTSETKKFLDSVSHDKTWWTSRYNPADDVPVSGIYRCTVCGK
                     EITSNKDDPFPPQNHHQHSQSQAIKWQLIVRTDTQGNGCGSK"
                     /name="protein L"
     Typing          425..509
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.393"
                     /coverage="87.0"
                     /evalue="5.15e-16"
     Typing          complement(801..882)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.857"
                     /coverage="98.0"
                     /evalue="1.43e-25"
     Typing          1183..1706
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.604"
                     /coverage="99.0"
                     /evalue="7.85e-161"
ORIGIN
        1 aaattcaggg tatcaggctg cgcctgatga aatgtttttg gtgccggcgg tactccaaaa
       61 acacatcttg tccgacagtt ttgtggtgcc ggcgaagcgg cggtggccac aaaacatttc
      121 ctgtccccat cgattcattc gagaaacata agaggcgaag aaggggtcag tttggagatc
      181 ggaaattttt gtacgttaag cgcattattt aaccaatctt ggttcgtaag gggttgattt
      241 tgcgtcagtg gctatcgctt ttaagttgtc gctcttgcag tatcgcattt aggttatccg
      301 attaaggtca aacctctgaa aatgccgtat agcgcgggag tacaccctat tgctataggt
      361 aagtctgttc aaaaaacagg cttaccgtac aataattctc tatatccaaa ctgaccccta
      421 aagaaacagg acaggatgtg caattgtaat accgtcgccc gcaacgtctt acaattacca
      481 cctggtcagg gtccacccga cacccggtac tgacggagaa caacggaagg tgaacaaaca
      541 gcaacaaatt gcgctgaata tggcgcgatt tatcaaaagc cagagcctga cactacttga
      601 aaaactggat gctctggatg ccgacgaaca ggcgaccatg tgcgaacgac tgcacgaact
      661 cgcggaagag ctccagaaca gcatccaggt tcgctttgag accgaaagtg aaacagggac
      721 ataacgaagc tgacggaggc gctcacggct tgtccgcgag cgttttcggc agcagacaag
      781 cccgtcaggg cgcgtcagtg gggttcgggg cgaagccctg aaccagtcac gtagagatag
      841 cggagtgtat acgggcttaa tcatgcagca tcagtgcgga ttgtatgaaa agtttgccat
      901 atccggagtg aaatgccgca cagatgcgta aggagaatat gcctgttcag gcgctcttcc
      961 gcttcctcgc tcactgactc gctacgctcg gtcgttcggc tgcggcgagc ggtgtctgct
     1021 cactcaaaga tggtaataca gttatccaca gaatccgagg ataacgccgg aaagaacatg
     1081 tgagcaacaa gacgaaaccc ggaaaaaggc agcgccggtg gcgtttttcc ataggcttcg
     1141 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1201 acttaaagat accaggcgtt tccccctggt ggctccctcg tgcgctctcc tgttcctgcc
     1261 attcggttta ccggtgtcac tccgctgtta tggccgcgtt tgtctcattc cacgcctgac
     1321 actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca
     1381 gtccgactgc tgcgcctttt ccggtaacta tcgtcttgag tccaatccgg aaagacacga
     1441 cttatcgcca ctggcagcag ccattggtaa caggattcac ggagcagagt agtagaagtg
     1501 cttctacaga gttcttgaag tgatggcctg actgcggcta cactggaagg acagttttgg
     1561 tatctgcgct ccgtgaaagc cagttacctt cggaaaaaag agttggtagc tcttgattcc
     1621 ggcaaacaaa ccaccgttgg tagcggtggt ttttttgttt gcaggacatg agattacgac
     1681 gagagtaaaa ggatcacaag aagatccggc tgtttaccag tgatttggct tcagtattgg
     1741 ctttcgccta gtggggactt tatctggcaa taaagctaat cttaaggctt cataggatac
     1801 atatggcaag ctccaatccc ctaaactagc cagactacta atcactcgac tagtatcttg
     1861 ttcccttggc actacaattc ctatttttaa ctcaggtcga tcggctttta tcatttctat
     1921 tggcggtatc atatctgagt cgttagtaca gagaaccaat tgttctgcct caccacggat
     1981 cgcatcgcga tacatttcta aagccattct aacgtcggtc tgcttttctt ctaacttcca
     2041 gaccgttgtt ctatccgttt ttgatggcgg atgcccctca ttaaaggtca ttgcacattc
     2101 ctgacgctct gagtaataac ctttgattat ctgtaggtta tcgccataga tctgtactaa
     2161 tgctcgatgg taggcttgct gagcctggtg cgccaattca ccccgagaag caaactttgc
     2221 tttgatctca gcagtaaaga acttcacctg aatcaactgt gactctcttg atcttatcgg
     2281 aagtagttgt tcttcaaaga gtttatagag atctaaccac ttatatcccg aacgcttaac
     2341 caatccgtga taaaaattaa acccatctac gtaaactatt gttctcattt tatttcgcgc
     2401 ataaaaaaac cgcctctaaa ggcggtttta acaacaaaaa ctaacgttag caaacagttt
     2461 aagttgtagt tcctcagggt taaaactaac cgagagggcg gtttcaagct cctgaaggaa
     2521 gacgcgtaac ttaatcgctg cccacctaaa gggaggttta gagcctcaag ggagctgcgt
     2581 aacttaatcg cgctaccaag aggtgagtgt agattaagta tagttaaata taatgatatt
     2641 ataagcgcga tggtgtcgta ctaattatat gaattaattt accaacctgc taagctgaag
     2701 tcttctatga agacttcagc ttagcaggtt ggtatatgtc agatcaaaaa aataatttag
     2761 accttcaaaa acagacactt gaatctaaat gggggaagga ggcgttaagc atgggatggt
     2821 cagccattcc tacatccctt ttcttcctgc agggcgcact caatatgacc cccacaacat
     2881 tcaatgtgct acttcatcta atcatccact ggtggaaacc ctatgattgg ccacatccat
     2941 ctcaagagag catcgcggtt agaatgggag tttcatcaag aacagttcaa cgctcactcc
     3001 aagaacttga aaattacgga ttgcttacaa aaattcgtac aagcaaggac aaccctaagt
     3061 acaaagggcg aaacatttac gatatgtctc ttttagttaa gtcactaaac attcttacgc
     3121 cagaccttaa tgccaaactg aacgaaaagt aaccataaaa taattttaaa aatcagacca
     3181 ttacagctta tatttaccat agcatctcta ttttagcgtt tttcaatcat cgccagatga
     3241 ttgaaaaacg ctaaaaaaaa ccaaacttat aaaaaggatc cccctccgcc ctatacattt
     3301 tggaaaggaa aggcaaatgg ctgtatacac ctctgagact aaaaaatttt tagacagtgt
     3361 ttctcacgat aaaacttggt ggacatcacg atataaccca gctgacgatg taccggtatc
     3421 agggatctat cgttgtactg tttgcggaaa agagataact agcaataaag atgacccgtt
     3481 tccgccgcaa aaccatcatc aacacagcca aagtcaggca atcaagtggc aattaattgt
     3541 cagaacagat acgcaaggaa atggttgtgg ttctaaataa ctcaaaagcc cttaggggct
     3601 tttttgtacc tagcggtcag acggattaaa cataacatcc gttgcccgca ccccctgaga
     3661 ggccctcagc gcgtttctgc cgcgtcctct gcgtttccgg cacctttccc tgtccatatc
     3721 gtgaaaaaac gcgcctgatg atttctgcgc atctgcatgg ctatgcacga acatgcctgg
     3781 ccggaatttt agcggctaaa atcctgcgaa ctgctcaaaa ggctcgctga accccatcga
     3841 taacggtgaa cgggtgcaca gtttactgtg cggtcgtgag gcgttgagca acgagctttg
     3901 cgaggcggta ggggttctga gagccagctg caggctcagg agaaaacatt ctgttttcgg
     3961 ggcagatacc gcaaatgacg tcattttgcg gcgtctgccc tatcctgcat cgcaacgtct
     4021 aaaggatgtg agtacaaacg ccagcacttt gcgaatcctg ccctcgatct gaacaaaagc
     4081 aaaatttcgt acgtacg
//
