LOCUS       NZ_CP089513            10060 bp    DNA     circular CON 23-JUN-2024
DEFINITION  Klebsiella pneumoniae strain GD21SC417 plasmid pHNGS471-4, complete
            sequence.
ACCESSION   NZ_CP089513
VERSION     NZ_CP089513.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23799605
            Assembly: GCF_023650835.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Gao,X., Lv,L., He,X. and Liu,J.
  TITLE     Tet(X4)-producing Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Gao,X., Lv,L., He,X. and Liu,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2021) College of Veterinary Medicine, South China
            Agricultural University, NO. 483, Wushan Road, Guangzhou, Guangdong
            510642, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023650835.1-RS_2024_06_23
            Annotation Date                   :: 06/23/2024 05:43:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,716
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,444
            CDSs (with protein)               :: 5,444
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 68 of 148
            Pseudo Genes (incomplete)         :: 84 of 148
            Pseudo Genes (internal stop)      :: 35 of 148
            Pseudo Genes (multiple problems)  :: 31 of 148
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP089513.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="GD21SC417"
                     /host="bacteria"
                     /db_xref="taxon:573"
                     /plasmid="pHNGS471-4"
                     /geo_loc_name="China:Guangdong"
                     /collection_date="2021-01"
     gene            join(8827..10060,1..452)
                     /locus_tag="LT986_RS28395"
                     /old_locus_tag="LT986_28385"
     CDS             join(8827..10060,1..452)
                     /locus_tag="LT986_RS28395"
                     /old_locus_tag="LT986_28385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            462..719
                     /locus_tag="LT986_RS28400"
                     /old_locus_tag="LT986_28390"
     CDS             462..719
                     /locus_tag="LT986_RS28400"
                     /old_locus_tag="LT986_28390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            804..953
                     /locus_tag="LT986_RS28405"
                     /old_locus_tag="LT986_28395"
     CDS             804..953
                     /locus_tag="LT986_RS28405"
                     /old_locus_tag="LT986_28395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            2437..4401
                     /locus_tag="LT986_RS28410"
                     /old_locus_tag="LT986_28400"
     CDS             2437..4401
                     /locus_tag="LT986_RS28410"
                     /old_locus_tag="LT986_28400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            4401..5132
                     /gene="mobC"
                     /locus_tag="LT986_RS28415"
                     /old_locus_tag="LT986_28405"
     CDS             4401..5132
                     /gene="mobC"
                     /locus_tag="LT986_RS28415"
                     /old_locus_tag="LT986_28405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            5139..5669
                     /locus_tag="LT986_RS28420"
                     /old_locus_tag="LT986_28410"
     CDS             5139..5669
                     /locus_tag="LT986_RS28420"
                     /old_locus_tag="LT986_28410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5696..5875)
                     /locus_tag="LT986_RS28425"
                     /old_locus_tag="LT986_28415"
     CDS             complement(5696..5875)
                     /locus_tag="LT986_RS28425"
                     /old_locus_tag="LT986_28415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5901..6329)
                     /locus_tag="LT986_RS28430"
                     /old_locus_tag="LT986_28420"
     CDS             complement(5901..6329)
                     /locus_tag="LT986_RS28430"
                     /old_locus_tag="LT986_28420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(6493..6807)
                     /locus_tag="LT986_RS28435"
                     /old_locus_tag="LT986_28425"
     CDS             complement(6493..6807)
                     /locus_tag="LT986_RS28435"
                     /old_locus_tag="LT986_28425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6795..7073)
                     /locus_tag="LT986_RS28440"
                     /old_locus_tag="LT986_28430"
     CDS             complement(6795..7073)
                     /locus_tag="LT986_RS28440"
                     /old_locus_tag="LT986_28430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(7248..7613)
                     /locus_tag="LT986_RS28445"
                     /old_locus_tag="LT986_28435"
     CDS             complement(7248..7613)
                     /locus_tag="LT986_RS28445"
                     /old_locus_tag="LT986_28435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(7610..7981)
                     /gene="tolA"
                     /locus_tag="LT986_RS28450"
                     /old_locus_tag="LT986_28440"
     CDS             complement(7610..7981)
                     /gene="tolA"
                     /locus_tag="LT986_RS28450"
                     /old_locus_tag="LT986_28440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(8255..8500)
                     /locus_tag="LT986_RS28455"
                     /old_locus_tag="LT986_28445"
     CDS             complement(8255..8500)
                     /locus_tag="LT986_RS28455"
                     /old_locus_tag="LT986_28445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             3827..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1057..1635)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4402..5129
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 agaaattaaa agcggctcag gcatctttaa acgcaatgaa tgaatcctta tccagagata
       61 aggctgcact taccggggcg atggagagcc ggaaacaaaa ggagaaaaaa gcgaaggatg
      121 cagaaaataa gttaaatgag gaaaagaaca agcctcgcaa ggggactaaa gactacggcc
      181 atgattatca tccagtacct aaaacagaag acataaaggg acttggagag ttaaagcgag
      241 gtgatcctaa aacacctaaa caaggtggtg gtggtaagcg ggcccgatgg tatggcgata
      301 aaaaacgtaa aatttatgaa tgggattccc agcacggtga gcttgaaggg tatcgtgcca
      361 gtgatggcga acacctaggc gcatttgatc ccaaaacggg taagcaggtt aaagggccag
      421 atccaaaacg gaacattaaa aaatatcttt gagaggtaaa tatggggctt aaattacgtt
      481 tgacatggtt tgataagaaa actgaagagt ttaaagggga agagtattct aaagactttg
      541 gtgacgatgg ttctgtcatt gaaagcttgg gaatgccttt aaaggataat attaataatg
      601 gtgagtttga tttgaagaaa gaatgggttc ctttactaca gccatttttt aaaaatcaaa
      661 taaaacctga tgtggataat tgttatatag cttttgatta tcgggacggt aactggtaac
      721 aaaaaattat caaggatgat ttgaaatact gacttccggt gttcagggat gaatgccgga
      781 gtctttaaat acagggaaag tatatgaaaa aggtaaagac tatttttctt tttatattga
      841 ttgttgctgg ctttcttctg gccgcatgtc aggcaaatta tatccgggat gttcagggcg
      901 gaacggtggc accatcgtcc tcctctgaac tgacggggat cgcggttcag tagaaaagat
      961 caaaggatct tcttgagatc ctttttttct gcgcgtaatc ttttgccctg taaacgaaaa
     1021 aaccacctgg ggaggtggtt tgatcgaagg ttaagtcagt tggggaactg cttaaccgtg
     1081 gtaactggct ttcgcagagc acagcaacca aatctgtcct tccagtgtag ccggactttg
     1141 gcgcacactt caagagcaac cgcgtgttta gctaaacaaa tcctctgcga actcccagtt
     1201 accaatggct gctgccagtg gcgttttgcg tgctttcccg ggttggactc aagtgaacag
     1261 ttaccggata aggcgcagca gtcgggctga acggggagtt cttgcttaca gcccagcttg
     1321 gagcgaacga cctacaccga gccgagatac cagtgtgtga gctatgagaa agcgccacac
     1381 ttcccgcaag ggagaacggc ggacaggtat ccggtaaacg gcagggtcgg aacaggagag
     1441 cgcaagaggg agcgacctac cggaaacggt ggggatcttt aagtcctgtc gggtttcgcc
     1501 cgtactgtca gattcatggt tgagcctcac ggctcccaca gatgcaccga aaaagcgtct
     1561 gtttatgtga actctggcag gagggcggag cctatggaaa aacgccaccg gcgcggccct
     1621 gccgttttgc ctcacatgtt agtcccctgc ttatccacgg aatctgtggg taactttgta
     1681 tgtgtccgca gcgcccgccg cagtctcacg cccggagcgt agcgaccgag tgagcgagga
     1741 ggcgcaattt ccccggcacc tgcaagacgg tcaccggacg cgctgatgtg ggatattgtc
     1801 ccctgattaa gtctttctat tccctgacga tcaattatca ggataggtga accatgtcgg
     1861 tttttcgctg aacattttac gttaacctat ctctgttgct tttttctcct tgcggagaaa
     1921 tgcaagtcat ccccgctggg gcgtggctga cttcagggga aaagaccttg cttacgctgg
     1981 ctggacgcca ccgacgcacg gtcttttccc ctctttctgg ctctgtccgg ctggacgccg
     2041 gccacgccag aaattcaccc ggcccccgtc aggctcacgc ctgaccaccc acaaaaatcc
     2101 acggtgctgg acgcacctga cgaagcagca aagctgctcc tgattttgcg ggtggtcagc
     2161 cgctcgctat ggcgaactct gacgggggcc ggaacaccgg aaaagaccgc actggggtgc
     2221 cgccttttcc tctcacacgc tggggcgtgc tcgttgttcc tccggggctg cgccttttcc
     2281 gcatggggcg gaaagaggct ggcctccgcg gtgaaagctg aacgcaatca ccacggacgt
     2341 gaccgccgcc gccatgcctt ggggcatgtc cgcggcttgt gcagcggggg ctgcgcatca
     2401 cgccggtaaa cccgtcgtct gaaggagaga gattgcatgt ttaatacgga ttcgcttgcc
     2461 tggcagtggc atatcccgga gctggcccgg cagatttacc cgtgggtcag ctggctgtac
     2521 cgggagtatt acagtctggc cggttttttt ctggttatcg gcctgttttt cggtctggct
     2581 gaactctggg ccggccgaat gcgttttgtg gggctgagcc ataaggcgcg gcgggagtat
     2641 gccgatcgct ataagcgcga taataaccgg actgtgccat tttcggtgct ggcctcgctg
     2701 gattcacccg ggcggatggt cagggcacgg cgacatgtct ggaactacgc gtactggctg
     2761 accggcgctg ccggtctggc ccttctgggg gggctggttg tcgccgtgtt tatcgagagc
     2821 tggggagaaa ccgtcctgcg cccggacctc tatgcgaacg cagattatta cctgctgcag
     2881 ccggctaacc tgttcgggat agcctggtgt gccggagggc ttgtcgccgg cgtccttctg
     2941 gcgcggctgc tggcgttcct gctgcttgat ggtcattttg cggccgctga cgaggcggtg
     3001 aacgcgaaac tgaatcagga gagccggcgc agcagccagc ggaccggaga aatgacagac
     3061 gtccgccacc tgcatttcgg ggagccggta ccggttaacg cgctggccga tttttccacg
     3121 gagcaggccc ggaaacagca ggcggtgttt ctgggtaagg atgagcaggg ccagccggtg
     3181 ctggtccctc gtgatacctg gcgcaagacc aatattcaga tcctcggcct gcccgggagc
     3241 ggcaagagcg cgatggccac caacgcgctc attcgctgcg tacgcgattt cggggacgct
     3301 gtggtgtact tcgaccccaa aggggacgcg tgggcgccgc acgtcttccg ggcgcactgc
     3361 ccggacttca ccctgctgga tctgcggccg gggaaaccgg cgcagctgaa tctgttccgc
     3421 gatctcgacc agtacgccct gaagaatctg ctggttgccg ggtttaacct gagtgagacc
     3481 ggcgacgtgg cggaccacta ccgcatcagc gagcagaaag cggcgaagct gattgcggag
     3541 cagttcccgc agggggcaaa cattcagcag gtgctggcgg cggcgtacgc gttgccggag
     3601 gccctgaaaa aggacgtgaa ggggctgatc accaagctgg agaacgtggc cgacctgagc
     3661 gtcctgcaga cggacagcgg tatcgatgtg gcggggataa tcaacggcgg cggctgcctg
     3721 tacgtcatcg gctcgatgga cgatgaggcg gtgatccggg tgcagaagat gctgtttgcc
     3781 cgctgcgcgc aaatcatcat tgcgcgggat gagttccgga ggtggccgca cgcgagcatc
     3841 atgctcgatg agattaaata tctgctgtcg aagtacgtgc tgaacgcgct ggggacgctg
     3901 cgcagccggg actgcaacct gctgctcgcg caccagtcgc tgggcgactt cggtcagtgc
     3961 gggcaggatc tgcccgctga ttttgtgaaa accacggtac tggataacac gccgatccgc
     4021 tggttctatc gcgcggccag ccaggagtcc gcgcagtggg cggcgggcca gaccggggag
     4081 atccgggtgg acgtggagcg ccgccgggcc agccgggagg cggggaacgt ggagcacatc
     4141 agcggcgaca gcttcatcca gaaggaggcg cggccgctgt ttgacgtgaa caccctgcaa
     4201 cacctgcctg acgggttcgc ggtgatgacc gggctgggcg tggcgcggct ggcgttcagc
     4261 agcccgctgc gggttgagcg ccgggaaatt tcgctgaaaa ccttcccggt gctggcgaaa
     4321 acggacccgc tggcggagta cctgcctccg gccgacggcg gcggaccaaa agcggatgat
     4381 gattttgcgg ggctttactg atgacactgg agcagtacaa cgtcagccac gcgaagcggc
     4441 aggcgcggaa cgcggagaaa acacggctga ccctgcgctg gctgcgggaa gagctgtgtt
     4501 ccacggcgga gctggttgcg cgacggctgg ggattgcggc ggtacagccg gtttaccgct
     4561 tcctggacag tctggtggcg aaagggctgc tggtccgggc gaaatacccg gttgacggcc
     4621 gtcaggtcag cgtgtggggg ctgacgccgc acggggtggc gttctcgttc gacgaggacg
     4681 agccgctgac ggacgttatt ccgtttcagc cgtcaagggt gtcggcggcg cagctgccgc
     4741 accggctggc cgtgcagtcc ctgcgcctct ccatggaggg ccggggggcc accggctggc
     4801 ggtacctgca ccgtatggcc ctgaaaggga tgaaagtgcc ggacgcgctg gcggagctgg
     4861 acggcagaac ggtggcattt gaggtagagc gcaccgtcaa gagccgcagg cggtatcagg
     4921 aagtggtgtc cgggtatctg ttcaaccgcc gggcgaacgg catcgatgaa atctggtaca
     4981 tctgccctga ccggccgacg cttgtccggg tacagcgggc gattctggcg gtggatgaaa
     5041 tcgtgaatcc acagaccggc gaggcccgca aaacggtgga gctggaccgg gagaggctgt
     5101 tcgcgtgctt taaatttatg acaacggagt gagggattat ggcgctgtac atggtattta
     5161 ttgaggacgt ggaagacaaa gacggcgagc gctgcattat gccaaccagc gagcatctgg
     5221 aagacatggt ggtgctcgcc tggcagaccg gggcgtatgt tttctgtttt gtcgaccggg
     5281 cccagcatct ggcgatgatg aaatccatcg ggatgtatga cgagcggctc aacagcgacg
     5341 aaaaagccac gtactttttc accggtgaaa atccgcaatg ggaacgggac aaacccacag
     5401 gaatgaagct gtgtgattac ctctatcgaa aggtggagcg gttcgccatc gagaacggtg
     5461 tgcaggtggt tgatgccggg aagtgctcac cggagcagat taacgcgatg attgaggtgg
     5521 tcagcgagcg gcacggggaa agcgcggtga acgagaagct gaaagcggag ctgatgcggg
     5581 aatataacag ccgctatttt aacgtgccgg tgcctgaact gccggaggac aaacccgccc
     5641 gccggtggtg gccgttcggc aaaaaataac gccagcaccg gcatggccgg tgctgttatt
     5701 cttccccgag cttatcccgc atcacctgcc agagtgtttc cgcatcctcg tgaagccgtt
     5761 cgcacatgtc ggccggggta tccatgtcca gctcgttcag cttttccagc aacagcagcg
     5821 actgatcctt aatgaattta gccatattga gggcagtttt ttcctgcttc gtcatgttct
     5881 ccattccctt cggtaagttc tcagctaaac cagccggacc agaagcgttt tttcttctgt
     5941 gtcagttctg ttgtcaatct gtccacttcg gcctggagtt tccggcgctc atcatcgtgc
     6001 tggcggcgtt cttcttcccg ggatgtttta tcttccagca tcagggtgac agcctgccgg
     6061 agatcggcca gttccttttg cattgccaga agctgttcct gtgggaaatt tttttgtgta
     6121 ttttcacggt gtgaaatgcc gggaatttgt ttctcaggga tattcacatt tccgtataca
     6181 cggatcagtt ctgacacatc cacgacaggg ttgtttttcc cgtcacgtga cacggtaacc
     6241 tttccctgtt ttacatgatt gtatagcgtt cttctggtaa tgcccgcggc ttgtgctgcc
     6301 tgtgaaaggt tgagcaatgt tttcgccact gtatacccct gattttatcg ggtgtgaaag
     6361 gtgtgtaatt tctcacggta aatgtatcgc tgaattttac ccgtttttcc gttcaggatg
     6421 tggttttctg acgcgctgcg cttgtcaaac cggcgacggc cacgcaatga attgcgcccc
     6481 cgtcatcggt tattagaggc tgtctgcccc cggatacccg gattctttct gatgcctgac
     6541 ggtgacgatc acaatctggt ccatttcccg gtcgtggcgg tagagcatca catagccact
     6601 gtcgccaaac ccgatcacca gttccttata ttccagcggc agaaacggca ccgggcggcc
     6661 aatgtctggc aatgttttca gttgctggat agcccggacg atcacctcac ctccttttct
     6721 ggctgccagc cgatttttgg ttttgagaaa gtcctgaagg cgttgtaaat cgcgctgtgc
     6781 cagcgcggaa atcgttacct gtggcatggc ggagcatcct gctcgttctc agttccccag
     6841 gtacttatcc aggcttccgc ttcttccgca gtaaggtgca gacccgtttc ctgatagtgc
     6901 tgccatgcgg cctgtccgtc ccgcaggtac tggctgcgct tctcttcccg gtcgatgtat
     6961 tcggtgattg cctccagcat tagcgcgtgc gctgagcggt gccggtcatc ggccagcgtt
     7021 ttcagccggt ccttaagttc ttcatcgagc ctgatggatg ttgctgatga cataaaagcc
     7081 cctttgtagt catatgtatt acacatgact actttaatcc gtggcggcgc tatttgccag
     7141 aaatacggct tttgctgccc atttttcagc ttcgcttcca ctaatcaggg ctgctacagg
     7201 ctcactcatc atccaggact attgcagcct gcgggttaaa ggacctgtta ctggcggcaa
     7261 ccatcatcat tgagcttttg ggtggtgact ggtcgcggat cctgtaagcc gtcatttttc
     7321 agactgaatt caaaatttat aacctgtcgc attgccgggt gccctgcggg atatagccag
     7381 tgcctcattg ccgtcagcgt ttcccgaaaa aaaaggtgac agccggtaag cgacacgttg
     7441 tccactcgtc catcgtccag aatatcaaac gtggcggtca cctgaccttc gatgcgtttt
     7501 gctgcggcct gtaccgggta ggttggctgc agtgtggtta cgggttttag tggtatcgca
     7561 tgtaccggag tgaggaacat ggccgccagc actgtcagag taaaagcttt catggtctga
     7621 aatccagagg ggcgtttttg aatatttgcc aggtattttc atccggtgcc ggcggaattt
     7681 tggcgtgggt aacggctgat attgctgctt tacagagttt cggatctcct ccttcagcaa
     7741 tggcgctgtt cagagtgcca tcacgttgta tggatatccg cacggtgcac atttttcccg
     7801 cataggcctc tggttcaaac atctgcattt caacagcggt cttgatggcc gcagcgtagc
     7861 tactgatttt agatgactta tccagagtat aaattttcgg cggctgggtg gtggaatgct
     7921 tacaggccgt caggagtaag gggctgcaca atgccagcag gataaagggt attaaacgca
     7981 tcgggttgat gacctgtcag taaaaaatgt tacatgtata tttgtcactc tttacggcca
     8041 cgttcattta cataaacacg gcttatcgta tcgtttttac accaaacggc ccgccatgca
     8101 gttgcgtgca taggcatgca gcgggttaca atttaccggg cgcgtttccg gcggttttcc
     8161 gggtggtttg ttgcctgttt tatcccgtag ccgccggaaa cgccctcaga ccgtctgagc
     8221 ggtgcgggca atgcgacgtt atggtaaata ccccttaccg gcggcgctgt ggctgccagg
     8281 tgaaggtggt ttccgtagcc tcgtatttct cccgacgcat ttcctgcgcc cgcctggctt
     8341 cacgttcgtc ctgaatggca cgaagcaccc tttcccgcac ctgttgtttt accgcctttg
     8401 gcagcgtttt gttccctttc aggcccatgg ccagtgcctg agcgacaaag gcgtccgcca
     8461 ggcgcttttc ttcagcggga gtcagtttca ggtgcgtcat agtggcctcc gggcgtctca
     8521 ggtttcccga acagtttaac cgcaggtcct ctcctcatgc cagttagctt tcagatgtgt
     8581 ctataccatc tgaaatgtaa gtgtgatgaa tgtcttaaat aataaaattt gttgccatga
     8641 gaaattttcg ggaaaagacg cgatctgctt acaggtggct ccgggcgctt ttttgtgcct
     8701 gaagatgaga actaatctca tatattgaca cctgaaaacg ggaggagtaa ggtaataatt
     8761 atactgtgta tatgtacagt atgttggctt taatttattg ttttaaatgt caaagaggaa
     8821 aacggtatga gtggtggaga cggtcgaggt ccgggtaatt caggtctggg acataatggt
     8881 ggtcaggcca gtgggaatgt gaacggtacg tctggtaaag gtggcccttc atcaggtggt
     8941 ggtacggatc caaacagcgg gccgggctgg ggtacgacgc atacgccgaa tggagatatt
     9001 cataactata atccggggga gtttggtaac ggcgggagta aacccggtgg taatggtggt
     9061 aacagcggca atcatagcgg tagctctggt ggcggacagt cttcggccac tgcgatggcc
     9121 ttcggtctgc ctgccctggc tactccgggg gctgaaggac tggctttatc agtttccggc
     9181 gatgcgttgt cggccgccgt tgctgatgtg ctggctgccc tgaaggggcc gtttaagttt
     9241 ggtctgtggg ggattgcgat ctacggtgtg ctgccttctg agattgcaaa agatgatccg
     9301 aaaatgatgt caaaaattat gacgtcatta ccggccgatg cggtgacgga gactccggca
     9361 agtactttac cactggacca ggcgacggtt cgtgtcagac aacgggttgt ggatgtggtg
     9421 aaggatgagc ggcagcatat tgcggttgtc gcaggtcggc caatgagtgt ccctgtggtg
     9481 gatgcgaaac cgacaaaacg tccgggggta ttcagtgtgt cgattccggg tctcccgtct
     9541 ctgcaggtga gcgtaccgaa aggtgttccg acagcgaaag ccccgccaaa aggcattgtt
     9601 gctgaaaaag gtgattcacg tccggctggt tttacagccg gtggtaactc ccgtgaggcc
     9661 gttattcgtt tcccgaaaga gaccggacag aagccggttt atgtgtcggt gacagatgtt
     9721 cttaccccgg cacaggtaaa acagcgtcag gaggaagaaa agcgtcgcca gcaggcatgg
     9781 gacgccgctc atccggaaga ggggctgaaa agagactatg ataaagcgaa agctgagctg
     9841 gatgccgaag ataaaaatat tgcgacctta aacagccgca ttgcatcgac agagaaggcg
     9901 ctccccggtg caagggctgc tgttcaggaa gccgataaaa aggtaaaaga ggcagaggca
     9961 aataaagatg attttgtgac ttataacccg cctcatgaat atggctccgg gtggcaggat
    10021 caggttcgct atcttgataa ggatattcag aatcagaatg
//
