LOCUS       NZ_CP089444             4150 bp    DNA     circular CON 09-JUN-2024
DEFINITION  Klebsiella quasipneumoniae strain GLW9C22 plasmid pHNLW22-3,
            complete sequence.
ACCESSION   NZ_CP089444
VERSION     NZ_CP089444.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23802440
            Assembly: GCF_023650855.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Gao,X., Lv,L. and Liu,J.
  TITLE     new TMexCD1-TOprJ1-variant
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Gao,X., Lv,L. and Liu,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2021) College of Veterinary Medicine, South China
            Agricultural University, NO. 483, Wushan Road, Guangzhou, Guangdong
            510642, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023650855.1-RS_2024_06_09
            Annotation Date                   :: 06/09/2024 05:16:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,251
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,957
            CDSs (with protein)               :: 4,957
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 103 of 166
            Pseudo Genes (internal stop)      :: 39 of 166
            Pseudo Genes (multiple problems)  :: 35 of 166
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP089444.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="GLW9C22"
                     /host="bacteria"
                     /db_xref="taxon:1463165"
                     /plasmid="pHNLW22-3"
                     /geo_loc_name="China:Guangdong"
                     /collection_date="2019"
     gene            13..231
                     /locus_tag="LT990_RS26200"
                     /old_locus_tag="LT990_26195"
     CDS             13..231
                     /locus_tag="LT990_RS26200"
                     /old_locus_tag="LT990_26195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019454712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_250681206.1"
                     /translation="MNRMSVNLSPEMEKLIEATSQRENISKAEVIRKAFALLQISEEEK
                     QKGRSIGIIAEDKGTHEMKVVGKIIGL"
                     /name="hypothetical protein"
     gene            235..537
                     /locus_tag="LT990_RS26205"
                     /old_locus_tag="LT990_26200"
     CDS             235..537
                     /locus_tag="LT990_RS26205"
                     /old_locus_tag="LT990_26200"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_250681207.1"
                     /translation="MNTTNLNIESSSGIEVIKLDSVEDKLGTADFNMAKEKFSLAKNIL
                     LFLFALTIVIIMIRVEPGNKGSDEIKELFNTVFQSIVPMSSLVIGYYFGSKEDKE"
                     /name="hypothetical protein"
     gene            541..810
                     /locus_tag="LT990_RS26210"
                     /old_locus_tag="LT990_26205"
     CDS             541..810
                     /locus_tag="LT990_RS26210"
                     /old_locus_tag="LT990_26205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_250681208.1"
                     /translation="MAAYIISYDLVKAGSKDYSNLYNAIKSLGKWAKVVESTWVVVSEL
                     SSVEIRDSLSLHMDAADRIIVVKSGNVGAWKNVICSNEWLKTNL"
                     /name="hypothetical protein"
     gene            complement(1611..2021)
                     /locus_tag="LT990_RS26215"
                     /old_locus_tag="LT990_26210"
     CDS             complement(1611..2021)
                     /locus_tag="LT990_RS26215"
                     /old_locus_tag="LT990_26210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_048295909.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKGIKVID
                     TSELIRVFGSLDGVIDDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKARDEH
                     IDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2021..2212)
                     /locus_tag="LT990_RS26220"
                     /old_locus_tag="LT990_26215"
     CDS             complement(2021..2212)
                     /locus_tag="LT990_RS26220"
                     /old_locus_tag="LT990_26215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032421723.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2942..3997)
                     /locus_tag="LT990_RS26225"
                     /old_locus_tag="LT990_26220"
     CDS             complement(2942..3997)
                     /locus_tag="LT990_RS26225"
                     /old_locus_tag="LT990_26220"
                     /inference="COORDINATES: protein motif:HMM:NF014074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_250681205.1"
                     /translation="MEPNIQDNQGDVDSGLPEGMQIVPDQNPLQSVNTEDVNESNEHAY
                     DLNRIDSIVSLLKCGNDEFIKKEINKHILGLMSKDEFCKLYNVVFLFDVVNSISRVHAE
                     KIYSSIQGFEEKKDIILFLRSRGGKVEPSYLISQLCNKFKRNKFIVSIPAEAKSAATLL
                     SFGADEIHMGPMSELGPIDLQVDGLPLQSISGALEKIAAIVQKFPESSDMFAKYLTDNL
                     NIGLIGYYDRVSESASQYAQMLLKGKHDDNTRSVEEIAAHFTNHYKDHGFVIDVAESTS
                     ILGEYMVKDNTQIYKSGSKVLEFFTNIEFTFALLGQRKSIVFVGNSCEIMTGNSFLGGV
                     KDEASFRNIKS"
                     /name="hypothetical protein"
     Typing          2333..2734
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.852"
                     /coverage="99.0"
                     /evalue="3.62e-158"
ORIGIN
        1 tgaggcgaaa aaatgaacag aatgagcgta aatttatcac ctgagatgga gaaacttata
       61 gaagccacat ctcaaagaga gaatatatcc aaggctgaag taattcgcaa agcttttgct
      121 ttactgcaaa tatcagaaga agaaaaacaa aaaggtagat ctatcgggat aatagccgag
      181 gataaaggga cgcatgaaat gaaggtggtc ggtaaaatca tagggctttg atttatgaac
      241 actaccaatt taaatattga aagctcatcc ggcatagagg ttataaagct ggatagtgtt
      301 gaagataaat taggcactgc cgatttcaac atggcaaagg agaagttttc gcttgcgaaa
      361 aacattctct tatttttgtt cgccctcaca atagtaatca ttatgattag agttgagcca
      421 ggaaacaaag gtagtgatga gataaaggaa ttatttaaca ctgtctttca aagcatagta
      481 ccaatgtcat ccttagttat tggctactac tttggaagca aagaggacaa ggagtaaatg
      541 atggctgctt atatcatctc atatgacctt gtgaaagcag gaagtaaaga ttatagcaac
      601 ctttacaatg caataaagag tttaggtaaa tgggctaaag ttgttgaatc aacatgggtt
      661 gtagtatctg aattatctag cgtggaaatc agagactctt taagtctaca tatggacgca
      721 gcagatcgaa tcatagtggt gaaatctgga aacgttgggg cgtggaaaaa cgtcatatgt
      781 agtaatgaat ggctgaaaac aaacttataa taaaaaagct cgcgattcgc gagctttttt
      841 attgaacgtt actcatggtt ttatttccac tcttatgtag cacactttta acataacatc
      901 cgttatcagc accgcctgag aggccctcag ggcgtttctg acgggaaacc ggtaaaacag
      961 acaacaaacc acccgaaaac ccgccagaaa cgcgcaggct ctgtttttac ggcatgcatg
     1021 agtatgcgcg caagtgcata gcgcggaaat tcacgccatt cagccggggt aagtcctgtt
     1081 tccggcgttc ttcccatccg aaaacggtga acggggacgc aatttattgc gtggccgtga
     1141 agcgttgccg gcgcagccgg aaaaccggtt ttcagcacgt tcaggaatcg ggttcgtcga
     1201 cggccggcaa tcacgttatg gcaagtcgcc ggacgactca ccggcgattc gactcgtgac
     1261 ttgactctag agtcaccgcg gtcaaaaaaa cgccaaagcg acgattcgac tctagagtca
     1321 agtcgtcata agttactgat agtgtggagt gagtcgtcga tgactctatg acgagtcgtc
     1381 ggcaagtcgt acacgaatca ccgacgactt ggattgacct tacagaaggt aatactgcgt
     1441 aatttaataa aacctaacca actgataata aagagttatc tgcaaaattt taattttgcg
     1501 tggtgtgtgg gtatttttcg tggtgaggag tgttcccgga tctggcgatt tttgggaagg
     1561 ttcaggaacg ccccgggatg gccggagagg aaaactcaag catggcggga tcaggatttt
     1621 ttccagaatt tccaccacgg ggaatggggt ggtgaagaag gttcgtgctt gtgctccaga
     1681 agctgcatag cctgacgaag ggaatcaata tgctcatctc gagccttaac agccccttta
     1741 agaccatcgt tttcagcttc aagtaaagcg attctatgtt gtaaatcatg taaaccaacg
     1801 ctgttaatac ctgtagattc tgcgtcactt tttacatcat cgatgacacc atcaagagaa
     1861 ccaaacactc taataagctc tgatgtatca ataactttaa tacccttccc gtgctttaca
     1921 acagaggtgt aacttaactt accactgtta atcatcttgt aaatagtagg gcgactaacc
     1981 ttaacaagcc gtgctgcttc actaatagac actctggcca tcagttacca tcctgtaatt
     2041 catccagtct cgaggacagt gtacggaaaa gatgctccgc atcatcgtgg agtttctcac
     2101 aaaggtcggc ctcagcatcg agatcaagct cgttaagctt ctccagcagg agcagagact
     2161 gattctggat gaatttagcc atgttcagcg ctgttttctg ttgtttcgtc ataaggggcc
     2221 tcagagcgtc agtgctcaat gtagggatgt tctcttttcc gcttcctcgc tcactcagtc
     2281 gctacgctcc tgccgttcgg ctgcggcgag cggcaggagc atgcgtataa agctcgagaa
     2341 aaccgaactg tcgcgtgatg taaaaaggaa aggccgcatg gcggccgttt ttccataggc
     2401 tccgcccccc tgacaagcat cacagaattt gacgctcaaa tcagtggtgg cgaaacccga
     2461 caggactata aagataccag gcgtttcccc ctgatggctc cctcgtgcgc tctcctgttc
     2521 ctgccattcg gtttaccggt gtcattccgc tgttatggcc gcgttgtctc attccacgcc
     2581 tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg
     2641 ttcagtccga ctaccacgtc cgatccggta actatcaact tgagtccaac ccggaaagac
     2701 acaacaaatc gccagtggcg gtagccattg gtaaagggag tacaatcgca actgaatgag
     2761 agttcttgaa gtgatggcca aagagcggct aaactaaaag gacagtattt ggtttcagcg
     2821 gtcttgtaca ccctgttacc acggttcaga agttccccaa cttactgaac cataggaaaa
     2881 ccgcctcccc aggcggtttt tttttaaata ttaccttctt acattcgaga caacaagata
     2941 atcaagattt tatattccta aaggaagctt catccttcac tcccccaaga aacgaattac
     3001 ctgtcattat ctcacaggaa ttaccaacaa aaacaatgct tttcctttgc ccaagcaagg
     3061 cgaaggtgaa ttcaatgtta gtaaaaaact ctaggacttt agaaccagac ttatatattt
     3121 gagtgttatc cttaaccata tactcaccta atattgatgt agattcagcc acatcaataa
     3181 caaagccatg atctttataa tggttggtaa agtgcgcagc gatttcctct acggaacgag
     3241 tattgtcatc atgcttacct ttcaaaagca tctgagcata ttgagaagct gattccgaaa
     3301 ccctgtcata gtaaccaata aggcctatgt ttagattatc agttaaatac ttagcaaaca
     3361 tatcagatga ctctgggaac ttttgaacaa tcgcagcaat cttttctaag gccccagata
     3421 ttgactgaag aggaagtcca tccacctgca aatcaattgg ccccaactca ctcattggtc
     3481 ccatatgaat ttcatcagcg ccaaaagaaa gcaatgtagc agccgattta gcctcagcag
     3541 gtatcgaaac gataaattta tttcgtttaa acttattgca taactgacta attaaataag
     3601 agggctcaac tttaccaccg cgacttcgga gaaaaagaat tatatctttc ttttcttcaa
     3661 atccttgaat tgaagagtat attttctctg cgtggactcg acttatcgaa tttactacat
     3721 caaataaaaa aacaacattg tataacttac agaactcatc cttagacatc aagccaagta
     3781 tgtgcttatt aatttctttc tttataaatt cgtcattacc acattttagc agagatacga
     3841 tactatcgat tcgattcaga tcataagcat gctcattgct ctcattaaca tcttcagtat
     3901 ttacactttg caatgggttt tgatcaggta ctatctgcat accttcagga agcccactat
     3961 caacatcacc ttgattgtct tgaatgtttg gttccatatc ctgttttccc aaagcttagt
     4021 cttggtgcac cgctatgtat gctttaggta aagttttctc ccacccattg caaacacacc
     4081 atttatgcac cattatcaca ccaattagct gcaataatgg tgcattgtaa ctacttttgc
     4141 aacaggaaaa
//
