LOCUS       NZ_CP089434             9294 bp    DNA     circular CON 08-DEC-2023
DEFINITION  Klebsiella pneumoniae strain 1194 plasmid p1194_7, complete
            sequence.
ACCESSION   NZ_CP089434
VERSION     NZ_CP089434.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05193406
            Assembly: GCF_001718115.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Cerdeira,L., Wick,R., Lam,M., Wyres,K., Judd,L., Garcia,D.,
            Lincopan,N. and Holt,K.
  TITLE     One health approach allowed a genomic landscape insight of enhance
            virulence and persistence of carbapenem-resistant Klebsiella
            pneumoniae clonal group 258 in South America
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Cerdeira,L., Wick,R., Lam,M., Wyres,K., Judd,L., Garcia,D.,
            Lincopan,N. and Holt,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2021) Biomedical Sciences II, University of Sao
            Paulo, Professor Lineu Prestes Avenue, 1374, Sao Paulo, SP 04344070,
            Brazil
REFERENCE   3  (bases 1 to 9294)
  AUTHORS   Cerdeira,L., Francisco,G., Bueno,M.F., Garcia,D. and Lincopan,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2016) Biological Sciences II, University of Sao
            Paulo, Av. Prof. Lineu Prestes 1371, Sao Paulo, SP 04344070, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore PromethION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/08/2023 10:05:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,719
            CDSs (total)                      :: 5,597
            Genes (coding)                    :: 5,455
            CDSs (with protein)               :: 5,455
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 57 of 142
            Pseudo Genes (incomplete)         :: 89 of 142
            Pseudo Genes (internal stop)      :: 17 of 142
            Pseudo Genes (multiple problems)  :: 20 of 142
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP089434.1.
            Bacteria and source DNA available from 1194/11.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="1194"
                     /isolation_source="catheter tip"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p1194_7"
                     /geo_loc_name="Brazil"
                     /lat_lon="23.560035 S 46.64733699 W"
                     /collection_date="2011"
                     /collected_by="Hospital Infantil Menino Jesus"
     gene            complement(join(8986..9294,1..1377))
                     /locus_tag="A9X66_RS28455"
                     /old_locus_tag="A9X66_028455"
     CDS             complement(join(8986..9294,1..1377))
                     /locus_tag="A9X66_RS28455"
                     /old_locus_tag="A9X66_028455"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1780..2199
                     /locus_tag="A9X66_RS28460"
                     /old_locus_tag="A9X66_028460"
     CDS             1780..2199
                     /locus_tag="A9X66_RS28460"
                     /old_locus_tag="A9X66_028460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            2505..2768
                     /locus_tag="A9X66_RS28465"
                     /old_locus_tag="A9X66_028465"
     CDS             2505..2768
                     /locus_tag="A9X66_RS28465"
                     /old_locus_tag="A9X66_028465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            2758..3057
                     /locus_tag="A9X66_RS28470"
                     /old_locus_tag="A9X66_028470"
     CDS             2758..3057
                     /locus_tag="A9X66_RS28470"
                     /old_locus_tag="A9X66_028470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3119..3496)
                     /locus_tag="A9X66_RS28475"
                     /old_locus_tag="A9X66_028475"
     CDS             complement(3119..3496)
                     /locus_tag="A9X66_RS28475"
                     /old_locus_tag="A9X66_028475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            3565..3744
                     /locus_tag="A9X66_RS28480"
                     /old_locus_tag="A9X66_028480"
     CDS             3565..3744
                     /locus_tag="A9X66_RS28480"
                     /old_locus_tag="A9X66_028480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3772..4302)
                     /locus_tag="A9X66_RS28485"
                     /old_locus_tag="A9X66_028485"
     CDS             complement(3772..4302)
                     /locus_tag="A9X66_RS28485"
                     /old_locus_tag="A9X66_028485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4309..5040)
                     /locus_tag="A9X66_RS28490"
                     /old_locus_tag="A9X66_028490"
     CDS             complement(4309..5040)
                     /locus_tag="A9X66_RS28490"
                     /old_locus_tag="A9X66_028490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(5040..7004)
                     /locus_tag="A9X66_RS28495"
                     /old_locus_tag="A9X66_028495"
     CDS             complement(5040..7004)
                     /locus_tag="A9X66_RS28495"
                     /old_locus_tag="A9X66_028495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(8485..8634)
                     /locus_tag="A9X66_RS28500"
                     /old_locus_tag="A9X66_028500"
     CDS             complement(8485..8634)
                     /locus_tag="A9X66_RS28500"
                     /old_locus_tag="A9X66_028500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(8719..8976)
                     /locus_tag="A9X66_RS28505"
                     /old_locus_tag="A9X66_028505"
     CDS             complement(8719..8976)
                     /locus_tag="A9X66_RS28505"
                     /old_locus_tag="A9X66_028505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     BGC             1..6377
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7804..8382
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4313..5040)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 ttcctcattt aatttatttt ctgcatcctt cgcttttttc tccttttgtt tccggctctc
       61 catcgccccg gaaagcgcag ccttatccct ggataaggat tcattcattt cgtttaaaga
      121 tgtctgggca gcttttaatt tctcattctg attctgaata tccttatcaa gatagcgaac
      181 ctgatcctgc cacccggagc catattcatg aggcgggtta taagtcacaa aatcatcttt
      241 atttgcctct gcctctttta cctttttatc ggcttcctga acagcagccc ttgcaccggg
      301 gatcgccttc tctgtcgatg caatgcgact gtttaaggtc gcaatatttt tatcttcagc
      361 atccagctca gctttcgctt tatcatactc tcttttcagc ccctcttccg ggtgagcagc
      421 gtcccatgcc tgctggcgac gcttttcttc ctccagacgc tgttttacct gtgccggagt
      481 aagaacatcc gtcaccgaca catagaccgg tttctgtccg ctctctttcg ggaaacgaat
      541 aacggcctca cgggagttac caccggccgt aaaaccagcc ggacgtgaat cacctttttc
      601 agcaataatg ccttttggcg gggctttcgc ggccggaaca cctttaggta cgctcacctg
      661 cagagacggg agacccggaa tcgacacact gaataccccc ggacgttttg tcggtttcgc
      721 atccaccaca ggaacactca ttggccggcc ggtgacaacc gcaatatgct gccgctcgtc
      781 cttcacaata tccgccacac gtttagtgac gctgaccgtc gcctggtcca gcggcaggga
      841 gctgaccggc gtctccgtta ccgtgtctgc cggcaatgac gtcacgattt ttgacatcat
      901 tttcggatca tcttttgcta tctcagaagg cagcactccg taaatcgcaa tcccccacag
      961 accaaactta aacggacctt tcagggcagc cagcacatca gcaacagcgg ccgacaacgc
     1021 atcgccggaa acggataaag ccagtccttc agcccccgga gtggccaggg caggcagacc
     1081 gaaggccatc gcggtggccg aagactgtcc gccaccagag ctcccgctat gattgccgct
     1141 gttaccgcca ttaccaccgg gtttactccc gccgttacca aactcccccg ggttatagtt
     1201 atgaatatcc ccgttaggtg tatgcgtcgt accccagccc ggcccgctgt ttggatccgt
     1261 accaccacct gatgaagggc cacctttacc tgatgtaccg ttcacattcc cactggcctg
     1321 accaccattg tgtcccagac ctgaattacc cggacctcgt ccgtctccgc cactcatata
     1381 cttttcctct ttgactttta aaacaataaa ctaaaataaa atactgtaca tataaccact
     1441 ggttttatat acagtataaa aattacgctg ccttgttttt catgtcaagt ctgtaaattt
     1501 gcgtttttgt gaaaatgatc aaaaaaacag ccactcacac catgcgcaat cccctccaga
     1561 tgcaacaaaa accagcacaa acggcgccgt tctccggcct cagtatgttt tactgaaatc
     1621 attatatgag ataccagaaa gccccctccg ggacaggaaa agaaacgggg ggtctggttc
     1681 gggttgttcg gtcactgaac acggtcagaa actgatgata agatagcctg cggatatata
     1741 actcaatgag aaaaaagaat taaaggtaag gagacgctca tgagacgttc cggcagcaaa
     1801 ctgtgtctta tcgccatgat aacagcgaca gcaactctca ctgcctgtgc tccaaagggc
     1861 agcgtggaac agcatacccg gcattacgta tatgcttcag atgacggttt tgatcccaat
     1921 ttttccaccc agaaagctga cacaacacga atgatggtgc cttttttccg gcagttctgg
     1981 gatatgggcg caaaggataa agcgaccgga aaatcacgga gtgatgtgca gcaacgcatt
     2041 cagcagtttc acagtcagga atttttaaac tcactccaag gcacaacaca atttgcgggt
     2101 accgattacc gcagcaaaga cctcacaccg aaaaaatcaa ggctactggc tgacaccatt
     2161 tcagctgttt atctcgatgg ctatgaaggc agacagtaag tggatttacc ataacgcgtc
     2221 attacgcgca ccgctcagac gggctcaggg cgtttccggc ggctacggga taaaacaggc
     2281 aacaaccccc ccggaaaact gccggaaacg cgcccgataa attttatcct aatgcatgcc
     2341 tatgcacgca actgcatggc atatgttttg acgtaaaatg gatgccgtaa gccctttttt
     2401 accgactttc tgcttcatca gcctgactga cacgaccata ttctttcaga ccgccccctg
     2461 ctaaacttcc ttcatattgc cgcattaacc cgggaggctc cgcaatgagc aacactacac
     2521 actacgaaaa cgccaacttt cttcgggaac tggctgaaag cctgccccgc atactgccgg
     2581 aaggtggccc ggacaaagct gcgctgttgc agcgactggc taacgaagaa ctggcccagg
     2641 cagagtatga agatcaggtt cgcgccaaag tgaccgccgc ccgtgctgac acgcgtccgg
     2701 gtatgaccac ggaacaactc cgccagcgcc tgcatggccg ttatcaggaa ctgcgcgatg
     2761 ccgtatgacg tggaatggaa gcagggagcc atcgaggatg taacgacgct gtttgactat
     2821 atcgcggaaa attcatccct gtgggatgcg cagaacgtta ccgaccgggt tctcgctgcg
     2881 gcggacaaac tggccgactt cccgcgactc tatgaagtgg actcacgcta tggcgaggac
     2941 gttcgccgga tcagtctgat gggacagcat gtcctctatg atgttgatga ccagaccaga
     3001 aaagtccggg ttctggccgt ggtcggccag cgccagaatc cccatattgt tctctaataa
     3061 ccggtgacgg ggacgcaatt cattgcgtgg ccgtcgccgg ttggacaagc acagcgcgtc
     3121 agcgttttcc ccacggccac caccggcgcg gtgttccggc cggggtctct gctgtctccg
     3181 gtttgtgttc cagcagtctc atggactgca tttcctgccg tagcccggcc acctcctccc
     3241 tgagtgcttt tatctccgcg atcaacgcgt cggaggccat tttttcagtc tcacccgaca
     3301 gtctcaactc gtcccgctcg tgagtctcat cctgtcttaa ctcaccgtac acccggataa
     3361 gctcgctggt atccaccacc cggccgccat cagcttccgt acggtatgat acgcggcctt
     3421 tcgccatatc ccgatacagg gaacttcggg ctttacccgt cagttccgtc gcctgtctca
     3481 ccgtcaccca cgccattgtc tcaccccgct atttttgaga cagtatacct gacaggcgca
     3541 caccatgccc ggaaggaaag aatcatgaca aaacaggaaa aaacagccct caatatggct
     3601 aaatttatta aggagcagtc gctgctattg ctcgataaac tgaatgagct ggatatggat
     3661 accccggccg acatgtgcga gcggctacac gaggatgcgg aaacactctg gcaggtgatg
     3721 cgggataagc tcggggagga ataacagcac cggcatggcc ggtgctggca tttatttttt
     3781 gccgaacggc caccaccggc gggcaggttt gtcctccggc agttcaggta ccggcacgtt
     3841 aaaatagcgg ctgttatatt cccgcatcag ctccgctttc agcttctcgt tcaccgcgct
     3901 ttccccgtgc cgctcactca ccacgtcaat catcgcgtta atctgctccg gtgagcactt
     3961 cccggcatca accacctgca caccgttctc gatggcgaac cgctccacct tgcgatagag
     4021 gtaatcacac agcttcattc ctgtgggttt gttccgttcc cattgcggat ttttaccagt
     4081 gaaaaagtac gtggcttttt cgtcgctgtt gagccgctcg tcatacatcc cgatggattt
     4141 catcatcgcc agatgctggg cccggtcgac aaaacagaaa acatacgccc cggtctgcca
     4201 ggcgagcacc accatatctt ccagatgctc gctggtcggc atgatgcagc gctcgccgtt
     4261 ccggtcctcc gcgtcttcaa taaataccat gtacagcgcc ataatccctc actccgttgt
     4321 cataaattta aagcacgcaa acaaccgttc acgatccagc tctgccgttt tgcgggcctc
     4381 gccggtctgt gggttcacga tttcatccac cgacaggatt gcccgctgca ccctgacctg
     4441 cgtcgcccgg tccgggcaga tgtaccagat ttcatcgatg ccgttagctt tgcggttgaa
     4501 cagatacccg gacaccactt cctgataccg gcgacggctc ttgacggtgc gctccacctc
     4561 aaacgccacc gttttaccgt ccagctccgc cagggcgtcc ggcactttca tgcctttaag
     4621 actcagacgg tgcagaatgc gccagccggt ggcccctctg gcttccatat caaggcgcag
     4681 ggattgcacg gccagccggt gcggcagatg tgctgccgac acccttgacg gctgaaacgg
     4741 aataacatcc gccagcggtt cgtcctcgtc gaacgagaac gccacgccat gcggcgtcag
     4801 cccccacacg ctgacctgac ggccgtccac cggatatttg gtccggacca gcagcccttt
     4861 cgccaccagg ctgtcgagga agcggtagac cggctgcacc gccgcaatcc ccagccgccg
     4921 cgcaaccagc tccgccgtgg aacacagctc ttcccgcaac cagcgcaggg tcagccgggt
     4981 tttctccgcg ttccgcgcct gtcgtttcgc gtggctgacg ttgtacagct ccagcgccat
     5041 cagtaaatcc ccgcaaaatc atcatccgct ttcggcccgc cgccgtcggc cgggggccgg
     5101 aactccgcca ggggatcggt tttcgccagc accgggaaac gctgcaacgg gatttcccgg
     5161 cgctcaaccc gcagcggact gctgaacgcc atccgggcca cacccagccc ggtcatgacc
     5221 gcgaacccgt ccggcaggtg ttgcagggtg ttcacgtcaa acagcggccg cgcctccttc
     5281 tggatgaagc tgtccccgct gatatgctcc acgttccccg cctcccggct ggcacggcgg
     5341 cgctccacgt ccaccctgat ttccccggtc tgccccgccg cccactgcgc ggactccagg
     5401 ctggccgccc ggtaaaacca gcggatcggc gtgttatcca gtaccgtggt tttcacaaat
     5461 tcagcgggca gatcctgccc gcactgcccg aaatcgccca gcgactggtg cgcgagcaac
     5521 agattgcagt cccggctgcg cagcgtcccc agtgcgttca gtacatactt cgacagcaga
     5581 tatttaattt catcgagcat cacgctgacg tgtggccact cccggaactc gtcccgcgca
     5641 atgatgattt gcgcgcagcg ggcaaacagc attttttgca cacggatcac cgcctcgtcg
     5701 tccatcgagc cgataacgta caggcagccg ccgccgttga taatccccgc cacatcgatg
     5761 ccactgtccg tctgcaggac gctcaggtca gcgacgtttt ccagcctagt gatcaacccc
     5821 ttcacgtcct ttttcagggc ctccggcagc gcgtacgccg ccgccagcac ctgctgaatg
     5881 tttgccccct gcggaaactg ctccgcaatc agcttcgccg ctttctgctc gctgatgcgg
     5941 tagtggtccg ccacgtcgcc ggtctcgccc aggttaagcc cggcaatcag caggtttttc
     6001 agcgcgtact ggtcgagatc gcggaacaga ttcagctgcg ccggtttccc cggccgcaga
     6061 tccagcaggg tgaagtccgg acagtgcgcc cggaagacgt gcggcgccca tgcgtcccct
     6121 ttggggtcga agtacaccac cgcgtccccg aaatcgcgta cgcagcgaat gagcgcgttg
     6181 gtggccatca cgctcttgcc gctcccgggc aggccgagga tctgaatgtt ggtcttgcgc
     6241 caggtatcac gcggaaccag caccggcttg ccctgctcat ccttacccag aaacaccgcc
     6301 tgctcctgcc gggcctgctc cgaggaaaaa tcggccagcg cattaaccgg taccggctcc
     6361 ccgaaatgca ggtggcgcac gtcggtcatc tctcctgtcc gctggctgct gcgccggctc
     6421 tcctgattca gtttcgcgtt caccgcctcg tcagcagccg caaaatgacc gtcaagcagc
     6481 aggaacgcca gcagccgcgc caacacaatc ccgacagcca gcccgcccgc gcaccaggca
     6541 atcccgaaca ggttagccgg ctgcagcagg taataatctg cgtttgcgta gaggtccgga
     6601 cgcaggacgg tttctcccca gctctcgata aacacggcga caaccagccc ccccagaagg
     6661 gccagaccgg ccgcgccggt cagccagtac gcgtagttcc agacatgtcg acgtgccctg
     6721 accatccgcc cgggcgaatc cagcgaggcc agcaccgaaa atggcgctgg ccgtttatta
     6781 tcgcgcttat agcgatcggc atactcccgt cgctccttat gactcagccc cacaaaacgc
     6841 atccgtccgg cccaaagttc agccagaccg aaaaacaggc cgataactag gaaagccccg
     6901 gccagaccgt aatactcctg gtacagccag ctgacccacg gataaatctg ccgggccagc
     6961 tccggaatgt gccacttcca ggcaagcgaa tccgtattaa acatgttctc tctccttcag
     7021 acgatggttt gccggcgtga tgtgcagccc ccgctgcaca agccgcggac atgccccaag
     7081 gcatggcggc ggcggtcacg tccgtggtga ttgcgttcag ctttcaccac ggaggccagc
     7141 ctctttccgc cccatgcgga aaaggcgcag ccccggagga acaacgagca cgccccagcg
     7201 tgtgagagga aaaggcggca ccccagtgcg gtcttttccg gtgttccggc ccccgtcaga
     7261 gttcgccata gcgagcggct gaccacccgc aaaatcagga gcagctttgc tgcttcgcca
     7321 ggtgcgtcca gaaccgtgga tttttgtggg tggtcaggcg tgagcctgac gggggccggg
     7381 tgaatttctg gcgtggccgg cgtccagccg gacggagcca gaaagagggg gagagaccgt
     7441 gcgtcggtgg cgtccagcca gcgtaagcag gggatatccc cctgaggtca gccacgccct
     7501 cgcggggatg acctgtattt ctccgtaagg agaaaaaagc aacagagata ggttaacgta
     7561 aaatgttcag cgaaaaaccg atatgattca cctatcctgg taatttatta tcagggaata
     7621 taaagactta atcaggggac aatatgccac atcagcgcgt ccggtgtccg tcttgtaggt
     7681 gccggggaaa ttgcgcctcc tcgctcactc ggtcgctacg ctccggccgt gagactgcgg
     7741 cgggcgctgc ggacacatgc aaagttaccc acagattccg tgaataagca ggggactaac
     7801 atgtgaggca aaacggcaag gccacgcagg tggcgttttt ccataggctc cgccctcctg
     7861 actgagttca cataaacaga cgctttttcg gtgcatctgt gggagccggt ggctcaacca
     7921 tgaatcagtc aggagtggcg aaacccgaca ggacttaaag atcatcaccg tttccgatga
     7981 atcgctccct cttgcgctct cctgttccaa ccctgccgtt tcccggatac ctgttccgcc
     8041 tttctccctt gcgggaagtg tggcgctttc tcatagctca cactctggta tctcggctcg
     8101 gtgtaggtcg ttcgctccaa gctgggctgt aagcaagaac cccccgttca gcccgactgc
     8161 tgcgccttat ccggtaactg ttcacttgag tccaacccgg aaaagcacgc aaaacgccac
     8221 tggcagcagc cattggtaac tgggagttcg cagaggattt gtttagctaa acacgcggtt
     8281 gctcttgaag tgtgcgccaa agtccggcta cactggaagg acagatttgg ttgctgtgct
     8341 ctgcgaaagc cagttaccac ggttaagcag ttccccaact gacttaacct tcgatcaaac
     8401 cacctcccca ggtggttttt tcgtttacag ggcaaaagat tacgcgcaga aaaaaaggat
     8461 ctcaagaaga tcctttgatc ttttctactg aaccgcgatc cccgtcagtt cagaggagga
     8521 cgatggtgcc accgttccac cctgaacatc ccggatatag tttgcctgac atgcgaccag
     8581 aagaaagcct gaaaaaatca atataaaaag aaaaacaacc tttacctttt tcatatacct
     8641 tccctgtatt taaaaacccc ggtattcatc cctgaacacc ggaagtctgt atttcaaatc
     8701 atccttgata acttttgctt accagttacc atcccggtaa acaaaggata caaagtaatc
     8761 aaatttacta atatcgatta catttttaaa gtgtggctgt aatatcgaaa cccatgattt
     8821 ttcaacatca aaccaaccat tattaatatt atcctttaaa ggcatcccca gactttcaat
     8881 gacagaacca tcatcaccga agtcttttga gtattcacca cctttaaact cttcggtttt
     8941 cttatcaaac caatgaatat gtaatttaag ccccatattt acctcttaaa gatatttttt
     9001 aatgtttcgt tttggatccg gccctttaac ctgcttaccc gtttttggat cgaatgcccc
     9061 gaggtgttcg ccatcactgg cgcggtaccc ttcaagctca ccgtgctggg aatcccattc
     9121 ataaatttta cgctttttat caccatacca tcgggcccgc ttaccaccac caccttgttt
     9181 aggggtttta ggtttaccct ctttcaactc tcccaacccc ttaatatctt cagtcttggg
     9241 atcaggaaaa taatcatggc cgtaatcttt agttccctta cgaggttttt tctt
//
