LOCUS       NZ_CP089390             1780 bp    DNA     circular CON 17-FEB-2024
DEFINITION  Klebsiella variicola subsp. variicola strain KPN692 plasmid
            pKPN692_5, complete sequence.
ACCESSION   NZ_CP089390
VERSION     NZ_CP089390.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23286210
            Assembly: GCF_021228835.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella variicola subsp. variicola
  ORGANISM  Klebsiella variicola subsp. variicola
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Hawkey,J., Wyres,K.L., Judd,L.M. and Holt,K.E.
  TITLE     Whole genome sequencing of Klebsiella pneumoniae complex isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Hawkey,J., Wyres,K.L., Judd,L.M. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2021) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Assembly Name                     :: KPN692
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 5x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/17/2024 08:03:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,626
            CDSs (total)                      :: 5,505
            Genes (coding)                    :: 5,328
            CDSs (with protein)               :: 5,328
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 86 of 177
            Pseudo Genes (incomplete)         :: 102 of 177
            Pseudo Genes (internal stop)      :: 38 of 177
            Pseudo Genes (multiple problems)  :: 42 of 177
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP089390.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Klebsiella variicola subsp. variicola"
                     /mol_type="genomic DNA"
                     /strain="KPN692"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /sub_species="variicola"
                     /db_xref="taxon:2590157"
                     /plasmid="pKPN692_5"
                     /geo_loc_name="Australia: Melbourne"
                     /lat_lon="37.8136 S 144.9631 E"
                     /collection_date="2018-03-26"
                     /collected_by="Microbiology Unit & Department of Infectious
                     Diseases, Alfred Hospital"
     gene            complement(701..1690)
                     /locus_tag="LUX40_RS28025"
                     /old_locus_tag="LUX40_28025"
     CDS             complement(701..1690)
                     /locus_tag="LUX40_RS28025"
                     /old_locus_tag="LUX40_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_032219167.1"
                     /translation="MQNPKLQNLTDYSPADAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGETLNRMNAGFQRLKDRKEFSPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPSMLSGRDYIKHERWVDLWRGCLRVDYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESE"
                     /name="protein rep"
     Typing          784..975
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.409"
                     /coverage="100.0"
                     /evalue="6.18e-88"
ORIGIN
        1 ggtggtcacg cccccgaacg attgccctcg ttgcgggggt cgcttttaac gcgaccactc
       61 caaacagagt acttccaaac ccatttccaa gcaaatcacc tctcaggcct tgccgcacaa
      121 gggctgaggc cgaattccaa tcccagagtt atcccttaag tataaataca aggaacgcgc
      181 gcgaattttc cacctaattt ccccgtacat ccgccccgcc agaaaaccca aaaacgctta
      241 tcaggtggtt gcgctcactg cgtgatcaca accacctgcc ggcagtgttt tatgggtgca
      301 gtcgtcaatc actaaatcgg aacccactgg caagccagcg ggaccccacg atttagcgtt
      361 tgacgattcc agtaacggcg aacgctgaca gatttcaatc tggcggcgtg aggtgttggc
      421 agggtgtcgg ggcgcagccc tgacggatcg tttttggtac cggcatcgtg atggcggtac
      481 tccaaaaaca catcttgtcc cgttaaaaaa atccgtatta cggatcgcaa aaccctaagt
      541 tcggattccg aactcagtaa gcgattggcg tcactttgcg gcggtagccc tatcctgcat
      601 cggagtaaaa tcaatcggac aagatgtgac tttggtacac cgccaccaag gtgacggatg
      661 cccaaagtcc tcatggtcag ggcgcagccc cgacaccccg ttactctgat tcggttttat
      721 ctttcgtcgg tgagcgacgg tactttctga cctcagtttt ccactcaaac gcgatgcggg
      781 aaccgtcatc gtcgccctcg gaaatatcat ccccgatcac catgtctgcg tcggtttcct
      841 gatcgagctt cagtacgtct ttgagtgcgc cgccggtcgc cacaaatcgg cgcttgtgcg
      901 tctggcgggt cagttcgagg aaccattcag gatcggccac catgtcagcc ggctttgtgg
      961 aatatttcag ggtttcggcc actgcgccgc gcaccagttc ggcggtcgcg caggccagcg
     1021 attcgccgtc cttcggcttc cggggtttta ctgcccggac atcgacattc ggatcgtaat
     1081 ccacccgaag acacccacgc cacagatcaa cccagcgctc atgcttgatg taatcacgcc
     1141 cagacaacat cgatggcggc accatcatca gggtgtgaaa gtgcgggtgt gcgctgccgt
     1201 cgctcccgcg ggtgacttcc gtggtacgta tccagccctg cactggactg aactccttgc
     1261 gatccttcag tcgctggaaa cctgcgttca tccggttcag cgtctcgccc agctccccga
     1321 tcgcacagtt acgcacagtc agggtcagga acatccagcg ggcatcggga taatccgcca
     1381 cgatacgcgg cagggactgg tagaaacggg cttgccacat cagggagcgc ctccactggc
     1441 agacaggaca gtgccggaca cggcagaaat gcgcctcacg cagcctcagg cgcgtttctc
     1501 cggtttcttt gagggtagac cacccgaacc gcagcaaacc gccacaggag gccatacgcg
     1561 ccccgtagcg ctcatattca gccgcaagca ggtaaatccc tccaacgtcg tcagaaacgc
     1621 ttctgtgagc gtcccagggc gcatctgcgg gggaataatc cgtgaggttc tggagtttag
     1681 gattttgcat gcacaccgcc ccatactggg cgatggctca tttgacgtgt tatcattgcc
     1741 gtatcacctg tgttgtctgt cgcaaaatag aattcctaca
//
