LOCUS       NZ_CP089388             9448 bp    DNA     circular CON 17-FEB-2024
DEFINITION  Klebsiella variicola subsp. variicola strain KPN692 plasmid
            pKPN692_3, complete sequence.
ACCESSION   NZ_CP089388
VERSION     NZ_CP089388.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23286210
            Assembly: GCF_021228835.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella variicola subsp. variicola
  ORGANISM  Klebsiella variicola subsp. variicola
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Hawkey,J., Wyres,K.L., Judd,L.M. and Holt,K.E.
  TITLE     Whole genome sequencing of Klebsiella pneumoniae complex isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   Hawkey,J., Wyres,K.L., Judd,L.M. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2021) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Assembly Name                     :: KPN692
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 5x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/17/2024 08:03:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,626
            CDSs (total)                      :: 5,505
            Genes (coding)                    :: 5,328
            CDSs (with protein)               :: 5,328
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 86 of 177
            Pseudo Genes (incomplete)         :: 102 of 177
            Pseudo Genes (internal stop)      :: 38 of 177
            Pseudo Genes (multiple problems)  :: 42 of 177
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP089388.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9448
                     /organism="Klebsiella variicola subsp. variicola"
                     /mol_type="genomic DNA"
                     /strain="KPN692"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /sub_species="variicola"
                     /db_xref="taxon:2590157"
                     /plasmid="pKPN692_3"
                     /geo_loc_name="Australia: Melbourne"
                     /lat_lon="37.8136 S 144.9631 E"
                     /collection_date="2018-03-26"
                     /collected_by="Microbiology Unit & Department of Infectious
                     Diseases, Alfred Hospital"
     gene            101..214
                     /locus_tag="LUX40_RS27935"
                     /old_locus_tag="LUX40_27935"
                     /pseudo=""
     CDS             101..214
                     /locus_tag="LUX40_RS27935"
                     /old_locus_tag="LUX40_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            559..1674
                     /locus_tag="LUX40_RS27940"
                     /old_locus_tag="LUX40_27940"
     CDS             559..1674
                     /locus_tag="LUX40_RS27940"
                     /old_locus_tag="LUX40_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            1674..2306
                     /locus_tag="LUX40_RS27945"
                     /old_locus_tag="LUX40_27945"
     CDS             1674..2306
                     /locus_tag="LUX40_RS27945"
                     /old_locus_tag="LUX40_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            2672..2914
                     /locus_tag="LUX40_RS27950"
                     /old_locus_tag="LUX40_27950"
     CDS             2672..2914
                     /locus_tag="LUX40_RS27950"
                     /old_locus_tag="LUX40_27950"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(3260..3661)
                     /locus_tag="LUX40_RS27955"
                     /old_locus_tag="LUX40_27955"
     CDS             complement(3260..3661)
                     /locus_tag="LUX40_RS27955"
                     /old_locus_tag="LUX40_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3661..3852)
                     /locus_tag="LUX40_RS27960"
                     /old_locus_tag="LUX40_27960"
     CDS             complement(3661..3852)
                     /locus_tag="LUX40_RS27960"
                     /old_locus_tag="LUX40_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4620..5294)
                     /locus_tag="LUX40_RS27965"
                     /old_locus_tag="LUX40_27965"
     CDS             complement(4620..5294)
                     /locus_tag="LUX40_RS27965"
                     /old_locus_tag="LUX40_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_032419561.1"
                     /translation="MRTIVYVDGFNFYHGLVKRSGYKWLDLYKLFEEQLLPIRARESQL
                     IQVKFFTAEIKAKFASRGELAHQAQQAYHRALVQIYGDKLQIIKGYYSERQECAMTFNE
                     GHPPSKTDRTTVWKLEEKQTDVRMALEMYRDAIRGEVEQLVLCTNDSDMIPPIEMIKAD
                     RPELKIGIVVPREQDTSRVISNLASLGDWSLPYVSYEALRLALLPDKVPTRRKPILKPN
                     HW"
                     /name="NYN domain-containing protein"
     gene            5642..6058
                     /locus_tag="LUX40_RS27970"
                     /old_locus_tag="LUX40_27970"
     CDS             5642..6058
                     /locus_tag="LUX40_RS27970"
                     /old_locus_tag="LUX40_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283459.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032419562.1"
                     /translation="MSDQKNNLDIQKQTLESKWGKEALSMGWSAIPTSLFFLQGALNMT
                     PTTFNVLLHLIIHWWKPYDWPHPSQESIAVRMGVSSRTVQRSLQELENYGLLTKIRTSK
                     DNPKYKGRNIYDMSLLVKSLNILTPDLNAKLNEK"
                     /name="helix-turn-helix domain-containing protein"
     gene            6476..6739
                     /locus_tag="LUX40_RS27975"
                     /old_locus_tag="LUX40_27975"
     CDS             6476..6739
                     /locus_tag="LUX40_RS27975"
                     /old_locus_tag="LUX40_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_054175658.1"
                     /translation="MAAYTSETEKFLQSVSSDQTWWKSRYNPGDSVPHSGIYRCTVCGK
                     EITSNGSDPFPPQNHHQHSQQQAIKWQLIVRTDTKGDRFGIK"
                     /name="hypothetical protein"
     gene            complement(7289..7501)
                     /locus_tag="LUX40_RS27980"
                     /old_locus_tag="LUX40_27980"
     CDS             complement(7289..7501)
                     /locus_tag="LUX40_RS27980"
                     /old_locus_tag="LUX40_27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(7505..7990)
                     /locus_tag="LUX40_RS27985"
                     /old_locus_tag="LUX40_27985"
     CDS             complement(7505..7990)
                     /locus_tag="LUX40_RS27985"
                     /old_locus_tag="LUX40_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_232393347.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRINDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYRTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEDGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<8028..8705)
                     /locus_tag="LUX40_RS28225"
                     /pseudo=""
     CDS             complement(<8028..8705)
                     /locus_tag="LUX40_RS28225"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(8668..8988)
                     /locus_tag="LUX40_RS27995"
                     /old_locus_tag="LUX40_27995"
     CDS             complement(8668..8988)
                     /locus_tag="LUX40_RS27995"
                     /old_locus_tag="LUX40_27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_042863636.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(9097..9277)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="88.525"
                     /coverage="83.0"
                     /evalue="4.47e-55"
     Typing          2..223
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.413"
                     /coverage="99.0"
                     /evalue="3.98e-99"
     Typing          3974..4378
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.103"
                     /coverage="99.0"
                     /evalue="1.28e-167"
     Typing          9227..9445
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.455"
                     /coverage="99.0"
                     /evalue="3.1300000000000003e-75"
     Typing          complement(7174..8678)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.422"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac
       61 actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca
      121 gtccgactgc tgcgcctaat ccggtaacta tcgtcttgag tccaacccgg aaagacacga
      181 caaagcgcca ctggcagcag ccactggtaa ctgatttagg gagttagtct tgaagttata
      241 cgccagttaa agctaaacta aacgaacagt tttggtgact gcgctccctt taagccagtt
      301 acctcggttc atagagcgcc aactctactg aacctttcga aaaaaccgcc cgccaaggcg
      361 gtttttttcg ttcacagccg gaagcttacg gctgctgcca gataatctca agaagaaagt
      421 aaaaaaaatc gattaaaaca caagcaatca aagttaatct ttgacataaa gtctagctaa
      481 tcacaatgaa agctgtacat tttattaagg tactaacaaa tcgtatggca tataacaaaa
      541 acacgaacag aggctatcat gataaaggaa ataagtttaa agaacttcaa atgcttcaat
      601 gagctatatc taaagcaatt aaagacttta aatatcatag ctgggaaaaa caactacggg
      661 aaaacatcta tactggatgc tattttttgt ttttatgatg taaaaaaccc ggctgttttg
      721 ttaaacattc aagcattcag aaaagaaatg gcagaaataa acaaaaacaa gccattttgg
      781 gttagttact ttcatgatat ggacacatca caaaagatgt ccatagtcat tcgagatgaa
      841 cgctctgaag taacacaaac atatgaaaca gaaacaaatc agagattaga atcaagtttg
      901 tctctaaacg ttgacaccgt actatctaac caaaacatac ctcgtcaaac tatcggttct
      961 caggccacag taaactcttt aaaaataaat gtgaccgaga cacccaatgc gaaaaacagg
     1021 gtttccaata agcttttctc tatgcaattg aaagaagatg gcgtagaaat aaaatccgaa
     1081 ataaaaaacg aaagggatgg gattaattat aaatttaaga cagcaacaat aataacaaca
     1141 tccagaaaga taaacaaaga ggcaacaatt acaaatgtat ctagcctact aacacaaaaa
     1201 aggaaaaaag acatactaga aaacttaaaa aaaatagatg acagaattgt agatatagca
     1261 atatctgcga taggtaacaa taaggaaata tatttagaca ttggatttag tgagctaaat
     1321 gaaatatcaa tgcttggcga gggtatcagt cgagcattat cttttatcag ctcagtgctg
     1381 gtacaggaaa actcaattat tcttattgat gaaatcgaga acggaataca ttactcagta
     1441 attaaagaca taataaaatc attaatttca tctgcaaaac aaaacaacaa tcaaatattt
     1501 gcaacaactc atagtcaaga tgtgatacga gcaataaacg aaatagatag caaaaatgaa
     1561 gatatcgctt atattagact aggaagagaa aagaactccc taaaaccaac agccgtacaa
     1621 ttcaatatgg atgatttctc ctactcagtt gagaacgact gggaggtaag ataatgaaag
     1681 acataaataa tagcaaaaag atcattttcg ttgagggtga tgatgatttt cattttctat
     1741 gcaacctact tgaaatacaa ggcattaccg atgtttttgt cgaaaaaata aatggaaaat
     1801 caaaattaaa acaagcactt agagcattca aaaacattga ttctttcgat gaaaaagaaa
     1861 gtatttttat tatattagat gctgacacat cattttctga cacagaaaga tcaataattg
     1921 ccacattgcg agaactacaa atctcctccc ccagctcgca taacgagata gctatgaatg
     1981 gcaatacaaa aatttctttc tttataatgc caggaaaaga caagcaaggt gccattgaag
     2041 atctgataat tgaacacgca agcactcgag cgttatatga atacataata gaatttttcg
     2101 aaaaaattaa aaataataaa gatgacatct cagcgagtga caaagagtac acttatccaa
     2161 caaacgaaaa aaaagctaag gtacaagtct atcttagctg caatagagaa tccgactcaa
     2221 gaataggaat atccatgaaa aatggaataa taaacacagc agacaattgc tttgatgaaa
     2281 taaaagattt tatttcaaaa atataaatcc aaatcaagcc agcccttcac gggctggctt
     2341 atcaccaggg tgttattaaa cgccggataa cgtatagaaa gcatcataca ctttagttat
     2401 cagcaatctc aaacgtccta aagacatcac cagatgccaa aacaatgatt gtgcagatct
     2461 aatgtaaaag acccagcaca agatgctccc ttaagttata ttctctctat tcacttaaaa
     2521 caatttaaca taaaatcata attgtacgca ccgctaaaac ggactcagga cgtttctgac
     2581 gggaaaccgg taaaacaggc aacaaaccac ccgaaaaccc gccagaaacg cgcaggctct
     2641 gtttttacgg catgcatgag tatgcgcgca agtgcatagc gcggaaattc acaccattca
     2701 gccaggggaa gtcctgtttc cggcgttctt ctcctccgaa aacggtgaac ggggacgcaa
     2761 tttattgcgt ggccgtgaag cgttgccggc gcagccggaa aaccggtttt cagcaccttc
     2821 aggaatcggg ttcgtcgacg gccggcaatc acgttatggc aagtcgccgg acgactcacc
     2881 acacgattca ccggcgattc gaatcgtgaa ttgaatcaag agtcaccatg agcaaaaaac
     2941 acccaaagtg atgaattgaa tcaagagtcg attcatcata acaatatgat aataaagcgt
     3001 gattcacata tgaatcgtgg gcaattcgtc agcgattcgc catagagtcg tccacgactt
     3061 ggatctgcac tttgtaacac atcaccgcac atttattttt cattaaccca ttgttgtaaa
     3121 taaggaatct gcaaaatttt aattttgcgt ggggtgtggg tatttttagt ggtgaggagc
     3181 gttcagagat ctgacgattt ttgggaaggt tcaggaactc cctgggatgg ccggagagga
     3241 aaactgaagc atgacggggt caggattttt tccagaattt ccaccatggc gccggggatt
     3301 cagttaattt tgcctggtga ttttcaagaa ggagcatggc ctgtttcagg ctgtcaatgt
     3361 gcgaatcctt ggacaaaagt aaggtgttca ggtgttcaat ctcctgtttc agatgacgga
     3421 tcacctgttc actctggtca gcagaggctg tatcacgctg tccagagacc tgtccagaat
     3481 ggtgttcacc cttcggttgc tcaaaggggc caaagacacg taaaagctct gaagtatcta
     3541 ccatttttgc attgcgctca ccgctacagg tggacaggtc acctgcttta attaacctgt
     3601 gcagtgttgt tctactctta cccgtcagtc gggcagcctc actaatagac actctggcca
     3661 tcagttacca tcctgtaatg catccagtct cgaggacagt gtacggaaaa gatgctccgc
     3721 atcatcgtgg agtttctcac aaaggtcggc ctcgacatcg agatcaagct cgttaagctt
     3781 ctccagcagg agcagagact gattctggat gaatttagcc atgttcagcg ctgttttctg
     3841 ttgtttcgtc ataagaggcc tcagagcgtc agtggtcagt gtagggatct cctcttttcc
     3901 gcttcctcgc tcactcagtc gctacgctcc tgccgttcgg ctgcggcgag cggcaggagc
     3961 atgcgtagaa acgctcgaga aaaccgagta atcgcatgga ggaaagaagg aaaggccgca
     4021 cagcggccgt ttttccatag gctccgcccc cctgacgagc atcacggaat ctgacgctca
     4081 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctgatggc
     4141 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
     4201 ccgcgtttat ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
     4261 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttttccg gtaactatcg
     4321 tcttgaatcc aatccggaaa gacacgcaaa aacgccactg gcagcagcca ctggtaacag
     4381 gattcacgga gcagagtagt agagtgcttc tacagagttc ttgaagtggt ggcctgactg
     4441 cggctacact ggaaggacag ttttggtatc tgcgctccgt gaaagccagt taccttcggg
     4501 aaaaagagtt ggtagctctt gattccggca aacgaaccac cgttggtagc ggtggttttt
     4561 ttgtttgcag agcatgagat tacgacgaga gtaaaaggat cacaagaaga tccggctgtt
     4621 taccagtgat ttggcttcag tattggcttc cgcctagtgg ggactttatc tggcaataaa
     4681 gctaatctta aggcttcata ggatacatat ggcaagctcc aatcccctaa actagccaga
     4741 ttactaatca ctcgactagt atcctgttcc cttggcacta cgattcctat ttttaactca
     4801 ggccgatcag cttttatcat ttctattggc ggtatcatat ctgagtcgtt agtgcagaga
     4861 accaattgtt ctacttcacc acgaatcgca tcgcgataca tttctaaagc cattctaacg
     4921 tctgtctgct tttcttctaa cttccagacc gttgttctat ccgtttttga tggaggatgc
     4981 ccctcattaa aggtcattgc acattcctga cgctctgagt aataaccttt tattatctgt
     5041 agcttatcgc catagatctg tactaatgct cgatggtagg cttgctgagc ttggtgcgcc
     5101 aattcacccc gagaagcaaa ctttgccttg atctcagcag taaagaactt cacctgaatc
     5161 aactgtgatt ctcgtgctct tatcggaagc agttgttctt caaagagctt ataaaggtct
     5221 aaccacttat atcccgaacg cttaaccaat ccgtgataaa aattgaaccc atctacgtaa
     5281 actattgttc tcattttatt tcgcgcataa aaaaaccgcc tctaagggcg gttttaacaa
     5341 caaaaactaa cgttagcaaa cagtttaagt tgtagtacct cagggttaaa actaaccgag
     5401 agggcggttt caagctcctg aaggaagacg cgtaacttaa tcgctgccca cctaaaggga
     5461 ggtttagagc ctcaagggag ctgcgtaact taatcgcgct accaagaggt gagtgtagat
     5521 taagtatagt taaatataat gatattataa gcgcactggt gtcgtactaa ttatatgaat
     5581 taatttacca acctgctaag ctgaagtctt ctaagaagac ttcagcttag caggttggta
     5641 tatgtcagat caaaaaaata atttagacat tcaaaaacag acacttgagt ctaaatgggg
     5701 gaaggaagcg ttaagcatgg gatggtcagc cattcctaca tcccttttct tcctgcaggg
     5761 cgcactcaat atgaccccca caacattcaa tgtgctactt catctaatta tccactggtg
     5821 gaaaccctat gattggccac atccatctca agagagcatc gcggttagaa tgggagtttc
     5881 atcaagaaca gttcaacgct cactccaaga acttgaaaat tacggattgc ttacaaaaat
     5941 tcgtacaagc aaggacaacc ctaagtacaa agggcgaaac atttacgata tgtctctttt
     6001 agttaagtca ctaaacattc ttacgccaga cctcaacgcc aaactaaacg aaaagtaacc
     6061 aaaaattccg acagccaaag cgcgcacatc actgtacaaa aggtgttcag catcacattt
     6121 ctagaaaatg acaacagctc cctattgaaa gctaatttct aaactaaaca tcagtatcgc
     6181 tacacaaaca gcaatacgga caaaacacaa aaagaggaat tagcatgtct aaagcaccaa
     6241 ttggtacgac tgcttctacc ggacaaaagt gtcctgaaag tggtgtctgg aaagtcgtag
     6301 gaaccccttc cactacagcc ccgatagcaa aaggtaatgt aatgcctccg tacgcaggaa
     6361 aggctgtaac ctggatgtta acccaatatg cttgatattt agactggcaa cgaaaagccg
     6421 ccccagcggg cggcttttct atctctgccc tataaattca gaaaaggaaa gctaaatggc
     6481 tgcatatacc agcgaaacag agaagttttt acaaagcgtc agtagcgatc aaacatggtg
     6541 gaagtcacga tataatccag gagacagcgt tccccattct ggaatttatc gatgtaccgt
     6601 ctgcggaaaa gagatcacca gtaacggcag cgacccgttc ccgccgcaaa accatcatca
     6661 gcacagtcag caacaagcaa tcaaatggca attgattgtc agaacggaca ctaaaggtga
     6721 ccgtttcgga atcaaatgat tgagagccct ccggggcttt ttttgtacct gaagctcgaa
     6781 gatatttacc ataacatccc ttgcccgcac cgctccggac aggttcgcca caaacgggac
     6841 ggcagtcagg taaaaccggc aacaaaccac ccgatacacc gccccgttcg tgacagataa
     6901 attttccccg gctgcatggt catgcagcca gatgcataca gtctgatttc aggccagaat
     6961 ccggatgaac gccttaaaaa caggaaaatg cttctccagc cagtcccaca gctcggtcgc
     7021 cagatacagg cagttaagta cagcttcaat gacgttaagc aatttcataa ttccccctag
     7081 gtataggccg tgctcttttt gatcacgaac ctatttagcg ggagaaataa gagcccttct
     7141 tcgtgcggcc tgtcaccgga tgatactgtt cagcccagtg tcggaccgtc ataactccgc
     7201 tgacgggaac gggcttccgc cagctctttt tcgtgctgtc gctgctcgta tgcccgttcg
     7261 gcggccaccg ccatttcatg ccgctgcact actggctcaa acgctgtaac tgcctgctca
     7321 agttcgccac ctgctgactc aagcgcgtca cgcgctcgtt cagtgccgcg ttctcccgtt
     7381 gcataagccc agacatcttc tgccattccg cgaaggcgct ctcccactcg tccagccttt
     7441 gcgagtagtc ctgatgtagc tgctctaatg cggtcagcaa ctgtttttcc agttccgtca
     7501 tgggctattt cctctccagt accatccagt tcccgtcctc cccgtaagct cccgctttcg
     7561 ggttcacttt gacgcaggac agtttcttct ccccgcagag cgacagctgc acgccgtggt
     7621 tcttctccga cagcatggcc tgcgtccgct cctgcgcccg gatagtccgg taattcccgg
     7681 tgatctgctg cccctgccac cacagaatgc tcccgctcgt ggcgatcagc agtaccgaga
     7741 ccagggtgat ggtcagccac gtccgcccga ccatacgcag cacgctcagg cggttcgact
     7801 gcatcgccgc ggtcataccc tgctcgtggg cgctgatggc gtcgttgatt ctcttcgcgc
     7861 ttgcgctcag ttccgctttt acagacttct cgtgctcgct gaatgcggct ttcagcatct
     7921 cgccggtact ctgctgctgc gctttcgatt gctgctccaa gtccttcgcc agcgctaaaa
     7981 gactgctcat aaatggctcc cctcagtcgg atatttcgcc ccccgtccgg gtcggcaatg
     8041 ctgatactgt tttttgtggt tcggaccacc tcaaaacctg ccgctgtgag cgcccctgtg
     8101 acgtcctcgc gcgttttgag ctccccggat gaggctaagg ccagcaaccc ccgtgtaatc
     8161 gcctgagcgg cttccagctt cgtttccggc agtgcggacg gcgtgaccag taaccgccgg
     8221 ttctccgggg cgtttggatc atgcagcccc agtctgccgt tcaccacggt ctgccaggca
     8281 tcgatgcgcg gccggtcggc gcgatcgtag tacggctgca ggcgtttccc ggtcagcagt
     8341 tcagtgttgg ggatcaggaa attcagctcc agcctcccct tgtcggtatg ctccacccac
     8401 agaatgctgt actggtcttt atcgagtccg ggcatcagaa cccgctcaaa gctggccatg
     8461 atctgctccc gctgcccggg cggcagttca gcctccgcga acgacagcac gccggaggtg
     8521 tactttttcg catacggcga ggcatcgata agctcccgga cttcctccgg ttttccctgc
     8581 agcacggtcg ccccctcgcg ctgccggtct tttcccagca ggtaatccac cggaccggca
     8641 ccaccaccgc gcccccgggg gtgaaattta acgatcatca tccccccctt tttccagcac
     8701 cgcatgccgc agccgctcca gtcccgcgtc gattgccatc agggcggcca caatctgcac
     8761 ccggtcatga ccggcaccac cggcgttcac tttccgggcg atctggttca ggttgttccc
     8821 cataccggca agctggcgca gcagcgccgg cgatatcgac ggcagtctgc cggcacgcgc
     8881 cggcttctca tcgaggcagg tctgacgcat ccacgccgcc agctgcctgc cgtcgcaccg
     8941 ctcgagcagc cgccggtgtt cgtcttccgt cacccacatc gtgagcatct tgttgcgttt
     9001 gtctgccaga gtagcctcct gagaacaccg ggacgggcgg gaaatgtttc ccggtcggca
     9061 cggtgttgga caagccgcag gcgcgtcagt ggcttttagc gggtttcggg gcgcagccct
     9121 gaaccagtca cgtagcgcta gcggagtgta tactggctca atatgttacg gcggagtaac
     9181 actttatgaa agtgcttcat gtgacagggg gaaaatgcag ctacagggcg tacaggagaa
     9241 aatggaggtt acggtgatat gacgcctcct cgctcactcg gtcgcttccg ctcctgccgt
     9301 tcggctgcgg cgggcgttac gcacacacgg ataagagcaa aggcatatga catgacaagc
     9361 agggcgggaa aaggatgcgg cgctgccgtt tttccatagg ctccgccccc ctgacaagca
     9421 tcacaaaatc tgacgctcaa atcagtgg
//
