LOCUS       NZ_CP089387            10423 bp    DNA     circular CON 17-FEB-2024
DEFINITION  Klebsiella variicola subsp. variicola strain KPN692 plasmid
            pKPN692_2, complete sequence.
ACCESSION   NZ_CP089387
VERSION     NZ_CP089387.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23286210
            Assembly: GCF_021228835.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella variicola subsp. variicola
  ORGANISM  Klebsiella variicola subsp. variicola
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10423)
  AUTHORS   Hawkey,J., Wyres,K.L., Judd,L.M. and Holt,K.E.
  TITLE     Whole genome sequencing of Klebsiella pneumoniae complex isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10423)
  AUTHORS   Hawkey,J., Wyres,K.L., Judd,L.M. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2021) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Assembly Name                     :: KPN692
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 5x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/17/2024 08:03:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,626
            CDSs (total)                      :: 5,505
            Genes (coding)                    :: 5,328
            CDSs (with protein)               :: 5,328
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 86 of 177
            Pseudo Genes (incomplete)         :: 102 of 177
            Pseudo Genes (internal stop)      :: 38 of 177
            Pseudo Genes (multiple problems)  :: 42 of 177
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP089387.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10423
                     /organism="Klebsiella variicola subsp. variicola"
                     /mol_type="genomic DNA"
                     /strain="KPN692"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /sub_species="variicola"
                     /db_xref="taxon:2590157"
                     /plasmid="pKPN692_2"
                     /geo_loc_name="Australia: Melbourne"
                     /lat_lon="37.8136 S 144.9631 E"
                     /collection_date="2018-03-26"
                     /collected_by="Microbiology Unit & Department of Infectious
                     Diseases, Alfred Hospital"
     gene            complement(join(10379..10423,1..288))
                     /locus_tag="LUX40_RS27900"
                     /old_locus_tag="LUX40_27900"
     CDS             complement(join(10379..10423,1..288))
                     /locus_tag="LUX40_RS27900"
                     /old_locus_tag="LUX40_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016161430.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_008804165.1"
                     /translation="MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPV
                     TSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTIDMAARNGKRLERIPDAVVNEVLAR
                     LDAILS"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(289..546)
                     /locus_tag="LUX40_RS27905"
                     /old_locus_tag="LUX40_27905"
     CDS             complement(289..546)
                     /locus_tag="LUX40_RS27905"
                     /old_locus_tag="LUX40_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="WP_039104089.1"
                     /translation="MYKTRLKKVGGSIMLAVPPAVLKALELSIDSEVGMTIDNGCLIIE
                     PQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAVGKEIL"
                     /name="antitoxin"
     gene            1066..2328
                     /locus_tag="LUX40_RS27910"
                     /old_locus_tag="LUX40_27910"
     CDS             1066..2328
                     /locus_tag="LUX40_RS27910"
                     /old_locus_tag="LUX40_27910"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_203544300.1"
                     /translation="MHSTEYAVQQALFTVGTAPTRSKALASFEENQHLLKDWRILYADE
                     CKTGPFPLRRAQFSEKGFVSTLFSGHSLWVLDHDVISSSNTMMFQVGYGTYIDSNAASF
                     IRSLAYREEPKEHLLNFCRMLSDSFSFDELSNINPYLYLWEAQRDRTPETVTGIRETMA
                     ALFALSWIKQPLNAEWGKIYRKLYQQQAEKEADRFLYQFYQNLDAGLGKAIEDRVDLLE
                     AMLLRTKLIELSSKRSAQNKMNELVQFMHDELSTMMIRELLVCADILFREGKSKMSQKL
                     DGLQKKKSPFDVLRNCARDLNMLRSMDQLTNSIADNTNSAFYVANLITFDRDIIDIIQL
                     TELRAIALSRSSSDAFPIYNRQLDTWLSEKLGKKRLSGLENIFRKDGFDIRSRARSRSN
                     VKTILQEDRQKLACIIEKINS"
                     /name="hypothetical protein"
     gene            2474..8752
                     /gene="avs3a"
                     /locus_tag="LUX40_RS27915"
                     /old_locus_tag="LUX40_27915"
     CDS             2474..8752
                     /gene="avs3a"
                     /locus_tag="LUX40_RS27915"
                     /old_locus_tag="LUX40_27915"
                     /inference="COORDINATES: protein motif:HMM:NF041816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AVAST type 3 anti-phage nuclease/ATPase Avs3a"
                     /protein_id="WP_232393346.1"
                     /translation="MSDSLLVRTSRDGDQFHYLWAARRALRLLEPQSTLVAMTIEGAST
                     TEMGSQPVVEDGEELIDIAEYYGSNELATAKIVRYMQLKHSTLHSDTPFPPSGLQKTIE
                     GFATRYKALIKKIPAETLRTKLEFWFVTNRPVSSSFSEAINDAASQHVTRHPHDLVKLE
                     KFTGLQGAELSIFCQLLHIEGQQDDLWSQRNILLRESTGYLPDLDTEAPLKLKELVNRK
                     ALTESAANPSITRMDVLRALGVDETDLFPAPCRIERIENAVSRVQEAVLVQRVVEASGA
                     PVIIHADAGVGKSIFSTHIGEHLPTGSVSILYDCFGLGQYRNASSYRHHHRTALVQMAN
                     EMASRGLCHPLIPNAGTGISQYMRAFLHRLSQSISILRASEPLAVLCIIIDAADNAQMA
                     AEEIGETRSFIKDLIREKLPDGVCLVALCRPYRRELLDPPPEALTLSLQSFDRDETAAH
                     LHQKFPDASESDVDEFHRLSSCNPRVQVLSLSQNLPLSDTLRLLGPNPKTVEDTIGEVL
                     EKSIARLRDTAGISGRAQIDTICSALAILRPLIPLSVLSAISGVASSAIKSFALDLGRP
                     LIVSGETIQFFDEPAETWFQRRFRPSAAALQQFITKLKPLTKDSSYAASVLPALMLEGN
                     QLSELIELAISSQALPETSPVERRDIELQRLQFALKAALRTGQYQDAAKLALKAGGECA
                     GDNRQRVLLRDNIDLAAKFVGSSGVQELVSRNAFPDTGWLGSRNAYYAAILSEYPELSG
                     EARSRLRLTMEWLTNWSQLSDDERSRQNVTDRDRAAMLIACLNIHGAEAAARDLRRWRP
                     RELSFDAGRIVAMQLLAHARYDELDQLAIAAGNDISLVMGIVLEARKFHRPVAEQAIRR
                     TWHLLKSPRVTIKGRNSIYDQTIAAITGMVEMALRQSVCTETEALQLLDRYLPKVPPHV
                     LTSEYSKERVAYVQAYALQANLMGTQLTLSDLASAEVKKELIAGKTHSESDDLRQLKQY
                     SGVLIPWYNLRAKVILSKTEKANLENELNDTQKQSTAIKSYSYREHSLASNEIANVWFD
                     ILIEEGNASRDDLENIIKWSKHKGNRVFTPTFYRFSSVCAEISGLEGLSYHFAGLALSL
                     WKDEHSDAQVKADGYIDLSRSLISLDEPEAKEYFNQAIEVTNKLGDENLSRWEAILDLA
                     EYIAGKEQVPDEIAYKLARCAELTREYVDRDKHFAWSDTVEILAELCPSSAMAIISRWR
                     DRTFGDHRNILAWTIEELVKKNKINALDALPFITFENGWHKCGLLDSVLSTCTDEKDKI
                     AAFEVVCHYTKFDVQNIENLKKLDAIAISLGIEHSELKERISLLKRTEATSKKTNLSPH
                     DSERDHTQEWNSVFEDCDLSSIEGVSAAYEKFRSVPEFYSKETFIKKAISRVKTGKECS
                     FITAIGTIFHWGLYDFKYILESIPNEWTSRLSIKTVLASLIKEYCQRFCMRIGKSRFYE
                     IFPFGLASRLSGISEKEIFGIVLEAIAESPEPANADRLFSLPGLLVSKLKSNEALNVLS
                     YALGLFDEVLKDEDGDGPWNEKLSPPTHVEDSLAGYIWARLGSPEAEMRWQAAHAVLAL
                     CRMNRTSVIQGIFQHAINATTLPFCDRNLPFYTLHAQLWLMIAAARVALDDGKSLIPNI
                     GYFYRYATTDQPHVLIRLFAARTLLALHDSGLISISAKEEDKLRNINQSTTLPVLDEVE
                     EHRGEDSYTFGIDFGPYWLEPLGRCFGVSQKQLEPEMLRIIRDDLGYKGSRNWDEDERN
                     KRRYYNDRDNHHSHGSYPRVDDYHFYLSYHAMFMTAGKLLATKPQVTSRYDDVEDVFQD
                     WLRRHDISRNDNRWLADRRDIQPKERSSWLNGSSDNRNEWLASISEDVFNEALYDNAGL
                     LTVWGHWSDICSDRKESIAVHSALVSPERSLSLLRALQTTKNVYDYKIPDAGDSLEIDH
                     AYYKLKGWIKDITDYYGIDEFDPWAGNVRFPIPEPASFIIDAMKLTTDKDHRVWYSPSS
                     VEPAMVSSIWGHLSGKNDEEKSHGYRLCASIRFIKSALATFKMDLILEVDVDRYSRNSR
                     YERNNENELDSIPSSTRLFLFRHDGTIHTLYGNYRTGEKTS"
                     /name="avs3a"
     gene            8646..9419
                     /gene="avs3b"
                     /locus_tag="LUX40_RS27920"
                     /old_locus_tag="LUX40_27920"
     CDS             8646..9419
                     /gene="avs3b"
                     /locus_tag="LUX40_RS27920"
                     /old_locus_tag="LUX40_27920"
                     /inference="COORDINATES: protein motif:HMM:NF041817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AVAST type 3 anti-phage proein Avs3b"
                     /protein_id="WP_136027732.1"
                     /translation="MNSTVSLQALDSSSSDMTEPSTRYTAIIELGKKLVDELKLNDSVD
                     TLSKWMAHHIAELIYDAEHCTDDIVRTAKQAEIRDSIWSFWSNRYELPIGSRPFQELEP
                     ILRTLKGLDPENEQPRFFSPYRDLINVEKETSEVQKWLTAAKDIDSAAKILIDYCLSLA
                     AENAIDKSQEWVELAQKAGLNKDVDLLEIRIFQLRGNPANTDNPNNAQRRILEKRQKRL
                     EAFLSLGSQLNEQLKSQLEALPAIEDEPTDDDKDF"
                     /name="avs3b"
     gene            9522..9725
                     /locus_tag="LUX40_RS27925"
                     /old_locus_tag="LUX40_27925"
     CDS             9522..9725
                     /locus_tag="LUX40_RS27925"
                     /old_locus_tag="LUX40_27925"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_136027733.1"
                     /translation="MSISRFLIENNGSNQDNIKIALSKAYHLCKDAGLQRVSLLFPAKG
                     TFSHSDIARSLGMQATKALIKG"
                     /name="hypothetical protein"
     gene            10023..10202
                     /locus_tag="LUX40_RS27930"
                     /old_locus_tag="LUX40_27930"
     CDS             10023..10202
                     /locus_tag="LUX40_RS27930"
                     /old_locus_tag="LUX40_27930"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_136027734.1"
                     /translation="MSTGLSHPSDKDRAKRIFADLKKQGLKAPEEAIRQFAVNNGWDPV
                     RAQELASFAKKYVG"
                     /name="hypothetical protein"
ORIGIN
        1 ggcgtcaggt atgcgttcca gacgcttgcc gttccgggcc gccatatcga tagttctggg
       61 ctggtcgcag cgaataacgc ctgttgtttt ggtccccgcc tcttccagtg aaaccgtgaa
      121 gcctgctgta cgtgcaaagt ttccgccgct ggtgacggga accacgaccg gtagccgggt
      181 cagcttgtta aacgatgcct tcgatacaat gaggaccgga cgttttccac tttgctcgtg
      241 gccagcaatc ggatccagtg aaacaagcca gatttcccct ctgtccattt acagaatctc
      301 cttgcccacc gcaggcgcat caatccattc ccgatcctct tcgctcattt cggcgtgcgg
      361 atcgcactgc gccaacagtt cctcaagcga ataatggggc cgtttctgcg gttcaataat
      421 caggcagcca ttatctatag tcatacccac ttcgctgtct atcgacagct ccagcgcttt
      481 cagcacggcg ggaggaaccg ccagcatgat ggatccgcca acctttttca ggcgagtttt
      541 gtacatagag gacctccgaa tattatattt taatataaca tccagggtgg attgtgcaca
      601 attactcatc agattcatgg tgatttcctt ttcgtaatga agtgggctga aagccgttac
      661 ccatcggagg gcaggtccgg tagttttggg aggaggcgtt gccggtaaaa gggcgatcat
      721 cctgatacat caggcgcttc tttcaggtca tgaagcattt cgtgcaagcg ggagcttagt
      781 tagtggtaag aaggctgata atagaaaacc gttttgcggt tcaaagtatt atgtcggttc
      841 ggataaacga aatacggggc atgttcccga tcgggactgc ttcggaggaa catcagctac
      901 acaaaaaagc caacactgac cgggctagtt ttggccgtgt gaaagatgtt ttgagggaag
      961 ataagcacaa cttactaaaa caacttgtta tttcctgtct gacagtgttt attaaatcat
     1021 tcagcatact tagaaggtta cgaaaagtta tttcaaggag agaaagtgca ttctacagaa
     1081 tatgcggttc aacaagctct tttcacagtc ggtaccgctc cgacacggag taaagctctt
     1141 gccagttttg aggaaaatca gcatttatta aaagactgga ggatccttta tgctgacgag
     1201 tgtaaaacag ggccattccc ccttaggcga gcccaatttt ctgaaaaagg ttttgtatca
     1261 acattattct ctgggcattc attatgggtt ctggatcatg atgtcatttc atcttcaaat
     1321 accatgatgt ttcaggttgg ctacggtact tatattgatt caaatgcagc aagttttatc
     1381 cgttctttgg cctatcgtga agagcccaaa gaacatctgc tgaatttttg tcgaatgctt
     1441 tcagattcat tctcatttga tgaactatcc aatattaatc cttatcttta tttatgggaa
     1501 gctcagagag acagaacacc ggaaacggta actgggatcc gggaaacaat ggctgcattg
     1561 tttgctcttt cctggattaa acaacctctg aatgcagaat ggggaaaaat ttacaggaaa
     1621 ttataccagc aacaggcgga aaaagaggct gaccgttttc tgtatcaatt ttaccagaac
     1681 ttagatgccg gtttaggaaa ggcgatcgaa gaccgagttg acctactgga agctatgtta
     1741 ttaagaacga agttaatcga actgtcttcc aaaagatccg cccagaacaa aatgaatgaa
     1801 cttgtgcaat tcatgcatga tgaattatct accatgatga ttcgtgaatt acttgtttgt
     1861 gccgacattc tattccgtga aggaaaatca aagatgtcac aaaaactcga cggcttacaa
     1921 aagaaaaaga gtcccttcga tgtactgaga aactgcgcca gagatttaaa tatgctcaga
     1981 agcatggatc agttgactaa ctctatcgca gataacacaa attctgcatt ttacgtcgct
     2041 aatttaatta catttgatcg cgatattatc gatattattc aattaacaga actgcgtgct
     2101 attgctctaa gtcgctcatc ttcagacgca ttcccgattt ataatcggca actggataca
     2161 tggttatcag agaaattagg caaaaaacgc ttgagcggct tagaaaatat attcaggaag
     2221 gatggattcg atatacgctc cagagccaga agtcggagta acgtgaaaac gatactgcaa
     2281 gaagacagac aaaaactggc ctgcataatc gagaaaatca atagttgaac atttttttgc
     2341 cacaagacgc tttcctgaaa ctctggtcat tcttagcagg aaagcatttt tcctccatac
     2401 tctctggtta tagagtatta aaaaataagg ggttataatc ctcacagcct aaccgacata
     2461 aggacgatgc tcaatgtctg acagcctgct tgttcgcacc agtagagatg gcgatcagtt
     2521 tcattatctt tgggcggctc gccgcgccct tcgactactg gaacctcagt caactcttgt
     2581 tgccatgacc attgaagggg catcaacgac ggaaatgggc tctcagccag tggttgagga
     2641 tggggaggag ctgattgata ttgctgaata ttacggcagt aacgaactcg caacagcaaa
     2701 aattgttcgt tatatgcagc taaagcattc aacactgcac tcagatactc cattcccccc
     2761 tagtgggtta caaaaaacca tcgaaggttt tgcaactcgt tataaggcac ttataaaaaa
     2821 aataccggca gaaacgttac gcactaaact cgagttctgg tttgtgacga accgtccagt
     2881 cagtagcagc ttcagtgaag cgatcaatga tgccgcgagc caacacgtta cacgtcaccc
     2941 acatgatctg gtgaaacttg agaaatttac cgggcttcaa ggtgctgagt tatcgatatt
     3001 ctgccagctt ttacatatag aaggtcagca ggatgattta tggagccagc ggaatatcct
     3061 gctaagagaa tcaactggat atctccccga cctggatact gaagcccctc tgaaattaaa
     3121 agagctggtt aacagaaaag cgttaaccga aagcgccgca aatccttcaa ttaccagaat
     3181 ggatgtgttg cgtgctttgg gggtagatga aacagatctt tttcctgcgc cctgccgtat
     3241 tgaaagaata gaaaatgccg tttcaagggt tcaagaggcg gtgctggttc agcgtgttgt
     3301 tgaagcatcc ggtgcacctg tgatcatcca tgcagatgct ggtgtgggga aatcaatttt
     3361 ctctactcat ataggggagc atcttcccac tggttcagtt agcatcttat atgactgttt
     3421 cggactgggt cagtaccgta acgcttcttc ttaccgccac caccatcgta cagcactggt
     3481 tcagatggct aatgaaatgg catctcgtgg tctctgtcat ccattgatcc caaatgctgg
     3541 tactggcata tcccagtata tgcgtgcgtt tctgcatcgc ctttctcaga gcatttcaat
     3601 actccgggcc tctgagcccc tggccgtatt gtgtattatt attgatgctg cggacaatgc
     3661 acagatggcg gcagaagaaa tcggtgaaac gcgttctttt atcaaagatt taattagaga
     3721 aaagcttcct gatggagtct gccttgtcgc actttgccga ccttatagac gggaattgct
     3781 tgatccacct cctgaagcac tcacattatc cctacaatct tttgatcgcg atgagacagc
     3841 cgctcatctt caccaaaaat ttccagatgc cagcgaaagt gatgttgacg agttccatcg
     3901 tctaagctct tgcaatcccc gggttcaggt tctgtcatta tcacaaaatc ttccactgag
     3961 cgacacactg agacttttgg ggccaaatcc caaaacggta gaagatacta ttggtgaagt
     4021 actggaaaaa tccattgcac gcttacgtga tacagctgga atatctggac gagctcaaat
     4081 tgatacgatt tgttccgcac tggcaatatt acgtccatta attccattat ctgtgctatc
     4141 tgccatttcc ggagtagcta gttctgctat taaaagtttc gcacttgatc tgggacgtcc
     4201 attaatcgtt agtggcgaga ctattcagtt ctttgatgaa ccagccgaaa catggtttca
     4261 gaggcgcttt cggccatcgg ccgctgctct gcagcaattt ataactaaac tgaaaccact
     4321 aacaaaagat agttcctatg cagcatctgt tttacctgca ttgatgctgg aaggaaacca
     4381 gctttctgaa ctgatcgagc tagcgatatc ctcacaagct ctgcctgaaa ccagcccagt
     4441 tgaacgcaga gacatagaac ttcaaagatt gcagtttgcg ttaaaagcgg ccttacgcac
     4501 aggtcaatac caggatgcgg ctaaactggc gctgaaggca ggtggagaat gcgcgggaga
     4561 caacaggcaa agggtcctgc taagggacaa tatcgatctg gcagcaaaat ttgtgggaag
     4621 cagcggcgtt caggaactgg tttcccgtaa cgcatttcca gatactggct ggctaggctc
     4681 cagaaatgct tattatgccg caatactttc cgaatatcct gaactctcag gagaggcccg
     4741 cagtcgcctt cgactcacca tggagtggtt aacaaactgg agccaattat cagatgatga
     4801 gcggagcagg caaaatgtta ccgatcgaga cagagcggca atgctcattg cctgcctgaa
     4861 tattcatggc gcggaagcgg cagcaaggga tctcagaagg tggcggcctc gagaactatc
     4921 ttttgacgca ggaagaattg tcgccatgca gttactggcc cacgcccgtt atgatgaact
     4981 tgatcagttg gctattgcgg caggaaacga tatcagcctg gtcatgggaa ttgtactgga
     5041 agcaagaaaa tttcaccgtc cagtcgctga acaggcaatc agaagaacct ggcacctgtt
     5101 aaaaagccca cgagtcacta ttaaaggcag aaacagcata tatgaccaga caatagcagc
     5161 aatcactggc atggttgaaa tggcacttcg gcaatcagtt tgtacagaga cagaagctct
     5221 ccaattgctg gatcgttatt taccaaaggt tccccctcat gttctgacct ctgagtatag
     5281 taaagaaaga gttgcttacg tccaggcata tgctcttcag gcaaatctga tgggcactca
     5341 attaactcta agtgatttag cctcagccga ggtcaaaaaa gaattaattg ctggaaagac
     5401 ccatagcgaa tctgatgatc tgcgtcaact gaaacagtat agcggagtat taatcccctg
     5461 gtataattta cgagcaaaag taattcttag taaaactgag aaagcaaact tagaaaatga
     5521 actaaacgat actcaaaaac aatcgactgc tattaagagt tactcttacc gtgaacattc
     5581 attagcatca aatgagattg caaatgtatg gttcgatatt ctgatcgaag aaggcaatgc
     5641 ttcaagagat gatttggaaa acatcatcaa atggagtaag cataaaggaa atagagtatt
     5701 tacaccaacg ttttaccgtt tcagttcggt atgtgcagag atttcagggc ttgaaggact
     5761 ttcatatcac ttcgccgggc tcgccttgtc attatggaaa gatgagcact ctgatgctca
     5821 ggtcaaagct gacggctata tagacctttc ccgctcactc atttcacttg atgaaccaga
     5881 agctaaagaa tacttcaacc aagcgattga agttacaaat aagctgggag atgaaaattt
     5941 aagtcgatgg gaagcgatac ttgatcttgc tgaatatatt gctggtaaag agcaagtccc
     6001 cgatgaaatt gcctataaac tagcccggtg tgctgagctt accagagaat atgttgatcg
     6061 ggataaacac tttgcgtgga gcgatactgt tgaaattttg gctgagttat gtccatcttc
     6121 agccatggca ataataagtc gttggcgtga ccgtacattt ggcgatcaca gaaatatact
     6181 agcatggacc attgaagaac ttgtaaagaa aaataaaatt aacgcactcg atgcacttcc
     6241 tttcattaca tttgagaatg gctggcataa atgcggtttg cttgactcag ttttatccac
     6301 gtgtaccgat gagaaagata agattgcggc attcgaagtg gtttgccact atacaaaatt
     6361 tgatgtacaa aatatcgaaa atcttaaaaa gctggatgct attgctatat cattaggtat
     6421 tgaacactca gagttgaaag aaagaatctc acttctaaag cgtactgagg cgacttcaaa
     6481 aaaaaccaat ctttctccac atgacagtga gcgagatcac acccaagaat ggaattcagt
     6541 ttttgaggat tgtgatttat catctattga gggtgttagt gctgcatacg aaaaatttcg
     6601 tagcgttcct gaattctatt ccaaagaaac tttcatcaag aaagcaataa gccgtgttaa
     6661 gacgggcaaa gaatgtagtt tcattactgc cattggtact atattccact gggggcttta
     6721 tgattttaaa tatatccttg aatctatccc caatgaatgg acatctcgtt taagcattaa
     6781 aacagtcctg gctagtttaa taaaagaata ttgccaacgc ttctgtatgc ggataggtaa
     6841 aagtcgtttt tacgagattt ttccctttgg tctagccagc aggctttctg gtataagtga
     6901 aaaagagatt ttcggtattg tcctggaggc tattgcagaa tcaccagagc ccgcaaatgc
     6961 tgaccgtttg tttagccttc caggccttct tgttagtaaa ctgaagagta atgaagcgtt
     7021 aaatgtatta tcttatgcct tgggtttatt cgacgaggtg ctaaaagatg aggatggtga
     7081 tggcccatgg aacgagaaat tatctccgcc aactcatgta gaggattcac ttgcaggcta
     7141 tatttgggcg cgactgggtt ctccggaggc ggaaatgcgc tggcaggcag cacatgcggt
     7201 tctggcacta tgtcgaatga atcgtacaag cgttatacaa ggaattttcc agcacgcgat
     7261 aaatgctacc actttacctt tttgtgatcg caatctaccc ttttataccc ttcatgctca
     7321 attgtggttg atgatcgctg ctgcaagggt tgcgctggat gatggaaaat cgctgattcc
     7381 caatattggt tatttctacc gttatgcgac tactgatcag ccacatgtat taatccgtct
     7441 ttttgctgcc agaactttac ttgcgctgca tgatagcggc ctgatctcta tctctgcaaa
     7501 agaagaggat aaactccgaa atataaacca gtctacgact ctccctgtgc tagatgaagt
     7561 tgaagagcat agaggtgaag attcatatac ttttggtatc gacttcggcc catactggtt
     7621 agaacctctg ggccgttgtt tcggcgtatc tcaaaaacag ttagaacctg aaatgcttcg
     7681 cattattcgc gatgatcttg gttataaagg cagccgcaac tgggacgaag atgagcgtaa
     7741 taaacgacgc tattataacg acagagataa ccatcacagc catggttcct atccacgtgt
     7801 cgatgattac catttttatc tgtcatacca tgcaatgttt atgacagccg gtaagctact
     7861 agcaacaaaa cctcaagtaa ccagtcgcta cgatgatgtt gaggatgttt tccaggactg
     7921 gttaagaagg catgatattt ctcggaacga taatcgctgg ctcgcagatc ggagagatat
     7981 acagcccaaa gagcgttcca gttggcttaa tggcagttct gataatcgga atgaatggct
     8041 ggcgtcaatc tctgaagatg tatttaacga agcactatac gacaacgccg ggctattaac
     8101 ggtatggggt cattggtctg acatttgttc agatcgaaaa gaatctattg ctgtccattc
     8161 tgcgttagta tcaccggagc gatctttatc gctcctcaga gcattgcaaa caacgaaaaa
     8221 cgtatatgac tataaaatcc ctgatgctgg ggatagtctt gaaatagatc atgcatacta
     8281 caaactaaaa ggatggatta aagatattac tgactattat gggattgacg agtttgatcc
     8341 ttgggccggt aatgtaaggt ttccaatccc agaaccagcc tcatttatca ttgatgcgat
     8401 gaaattaact actgataaag atcatcgggt atggtattca ccttctagtg ttgaaccggc
     8461 gatggtttcc agtatctggg gtcatctatc aggtaaaaat gatgaggaaa aatcacatgg
     8521 ttataggcta tgtgcctcaa tacgcttcat aaaatcagca ttagcaacat tcaaaatgga
     8581 tctcatttta gaggttgatg ttgatcgcta ttcacgaaac agcagatatg aacggaataa
     8641 tgaaaatgaa ctcgacagta tcccttcaag cactcgactc ttcctcttcc gacatgacgg
     8701 aaccatccac acgctatacg gcaattatag aactggggaa aaaactagtt gatgagctga
     8761 agctaaatga ctctgttgat acattaagca aatggatggc tcatcacatc gcagagctca
     8821 tttatgatgc tgaacattgt acagacgaca tcgtccgtac agctaaacaa gcggagatta
     8881 gggactctat ctggtcattc tggtctaaca gatacgaatt gccaattggt agcagaccat
     8941 ttcaggagct cgaacctatt ctaagaacct taaaaggtct tgatcctgaa aatgagcaac
     9001 caagattttt ttcaccttac cgagatctaa ttaatgtaga aaaagaaacc agtgaggtcc
     9061 aaaaatggct aaccgctgct aaggatattg attctgcagc aaaaatactg attgattact
     9121 gtttatcgtt agcagcagaa aatgctatcg ataaatccca agaatgggtg gaattagcac
     9181 agaaagctgg attgaacaaa gatgttgatc tgcttgaaat tcgtatcttt cagttacgag
     9241 gtaatccggc caatacagat aatcccaata atgcgcaacg gagaatactg gaaaaaaggc
     9301 aaaaaaggct tgaagctttt ctctcattag gctcccagtt aaacgaacaa ctcaaatcgc
     9361 agcttgaagc cttaccagca attgaggatg agccaacgga tgacgacaaa gacttttgag
     9421 atgacttgct ttagcactgg ggacggctca caagacagac cacataacag cctaacccaa
     9481 gactttacta ctattcctaa tgtttattaa aaggagtttt tatgtcaatt tccagatttc
     9541 taatcgaaaa taatggttca aatcaagata atataaaaat cgccttaagt aaggcctacc
     9601 acctatgcaa agatgcggga cttcaacgag tatctctgct atttcctgcg aaaggaacat
     9661 tttcacattc tgatatagca agatcccttg gcatgcaggc aacaaaggca ttgataaaag
     9721 gttaacctgt cgatctgggt aataatgttc gactggagtt taatatccct aaaaattttc
     9781 caatatatgg caatcatgac attgtattgg ctacatattt aacagataaa gacatggata
     9841 ttgttgatag tgttcaaaac atcaatagta tcgtcttttt gccatggtca gaggaggaag
     9901 gtatacgctg gctatctaca tgggagcctg aaatagttgg cccgtctaca tagaaaatcc
     9961 taaaaccaga attgccagac cctattagta atgaaatttt aagactcggt cgctgcatca
    10021 acatgtcaac agggcttagc cacccatctg ataaagacag ggcaaaacga atttttgctg
    10081 acctaaaaaa gcaaggactt aaagcacctg aagaagcaat cagacaattt gcagtcaaca
    10141 atggctggga ccccgtcaga gcgcaggagt tagctagttt tgccaagaaa tatgtggggt
    10201 aattttacct gttttttatt gcattccatt agtggtcatt ctggtggcct tgaaaacccc
    10261 aatttttagg tttagcttgt taatcagcca gttaatggct aagacgatcc cagtcagagg
    10321 agccacattt aaaaagcctg ctttcgagca ggctttttgc ttttttgcgt cgctgacgtt
    10381 aactcaaaat cgcatccagc ctggcaagca cttcattgac aac
//
