LOCUS       NZ_CP089323            11983 bp    DNA     circular CON 17-FEB-2024
DEFINITION  Enterococcus faecium strain NMVRE-001 plasmid p4, complete sequence.
ACCESSION   NZ_CP089323
VERSION     NZ_CP089323.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23828484
            Assembly: GCF_021228615.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11983)
  AUTHORS   Pincus,N.B. and Bachta,K.E.R.
  TITLE     Enterococcus faecium clinical isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11983)
  AUTHORS   Pincus,N.B. and Bachta,K.E.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2021) Medicine (Infectious Diseases), Northwestern
            University Feinberg School of Medicine, 645 N Michigan Avenue,
            Chicago, IL 60611, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-MAY-2021
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/17/2024 08:03:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,146
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,912
            CDSs (with protein)               :: 2,912
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 47 of 141
            Pseudo Genes (incomplete)         :: 88 of 141
            Pseudo Genes (internal stop)      :: 32 of 141
            Pseudo Genes (multiple problems)  :: 23 of 141
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP089323.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NMVRE-001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p4"
                     /geo_loc_name="USA: Chicago, Illinois"
                     /lat_lon="41.89 N 87.62 W"
                     /collection_date="2021-02"
                     /collected_by="Clinical Microbiology Laboratory at
                     Northwestern"
     gene            complement(join(11096..11983,1..729))
                     /locus_tag="LUB18_RS15555"
                     /old_locus_tag="LUB18_15555"
     CDS             complement(join(11096..11983,1..729))
                     /locus_tag="LUB18_RS15555"
                     /old_locus_tag="LUB18_15555"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_232407810.1"
                     /translation="MENIGLSEILYVQGNSNKLIPVLKKYGTPTISNINQSDTFQINTN
                     IVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTIKDSIKELNLLFCSLIITF
                     ILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIYHLSSIKNYLKNSRPLKLN
                     LCILNICLFLSMILLLIASTKVINIHNEAENNSLKYWERTTNLYKTNITNQLNRNNTVE
                     ENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFIGQKVYPNFEDYVSQPAGA
                     GICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKYKEYAKMIEKNYLEDIQFR
                     LEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYFSFNTLYGKAKDNYTITDPIVRVINPEI
                     TESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNLENIINFSYSVFQETGELL
                     SQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIALKKIFGYSPISQVLDLCL
                     LPFTIELFATMCFQLIFSKNT"
                     /name="hypothetical protein"
     gene            827..2005
                     /locus_tag="LUB18_RS15560"
                     /old_locus_tag="LUB18_15560"
     CDS             827..2005
                     /locus_tag="LUB18_RS15560"
                     /old_locus_tag="LUB18_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="WP_002324322.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDYE
                     KYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETV
                     IHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAIFMD
                     ATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDIYSRGVHDV
                     LLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRNIAHKVRVKDRKEICDDFKAVYQASS
                     KEEANTFLGSMIEKWQKTYPKVTQSLIKNQDLLTFYEFPPGIRRSIYSTNLIESFNKQI
                     KKYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE"
                     /name="IS256-like element ISEfm2 family transposase"
     gene            complement(2030..2470)
                     /locus_tag="LUB18_RS15565"
                     /old_locus_tag="LUB18_15565"
     CDS             complement(2030..2470)
                     /locus_tag="LUB18_RS15565"
                     /old_locus_tag="LUB18_15565"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_232407812.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVEGASIILCK"
                     /name="hypothetical protein"
     gene            complement(2524..2898)
                     /locus_tag="LUB18_RS15570"
                     /old_locus_tag="LUB18_15570"
     CDS             complement(2524..2898)
                     /locus_tag="LUB18_RS15570"
                     /old_locus_tag="LUB18_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000713874.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            3031..3492
                     /locus_tag="LUB18_RS15575"
                     /old_locus_tag="LUB18_15575"
     CDS             3031..3492
                     /locus_tag="LUB18_RS15575"
                     /old_locus_tag="LUB18_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000389746.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3804..3974)
                     /locus_tag="LUB18_RS15580"
                     /old_locus_tag="LUB18_15580"
     CDS             complement(3804..3974)
                     /locus_tag="LUB18_RS15580"
                     /old_locus_tag="LUB18_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(3990..4529)
                     /locus_tag="LUB18_RS15585"
                     /old_locus_tag="LUB18_15585"
     CDS             complement(3990..4529)
                     /locus_tag="LUB18_RS15585"
                     /old_locus_tag="LUB18_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(4557..5294)
                     /locus_tag="LUB18_RS15590"
                     /old_locus_tag="LUB18_15590"
     CDS             complement(4557..5294)
                     /locus_tag="LUB18_RS15590"
                     /old_locus_tag="LUB18_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            5939..6025
                     /locus_tag="LUB18_RS15595"
                     /old_locus_tag="LUB18_15595"
     CDS             5939..6025
                     /locus_tag="LUB18_RS15595"
                     /old_locus_tag="LUB18_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(6146..6835)
                     /locus_tag="LUB18_RS15600"
                     /old_locus_tag="LUB18_15600"
     CDS             complement(6146..6835)
                     /locus_tag="LUB18_RS15600"
                     /old_locus_tag="LUB18_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298772.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(6837..7115)
                     /locus_tag="LUB18_RS15760"
     CDS             complement(6837..7115)
                     /locus_tag="LUB18_RS15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            complement(7075..7989)
                     /locus_tag="LUB18_RS15605"
                     /old_locus_tag="LUB18_15605"
     CDS             complement(7075..7989)
                     /locus_tag="LUB18_RS15605"
                     /old_locus_tag="LUB18_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002348701.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(7971..8351)
                     /locus_tag="LUB18_RS15610"
                     /old_locus_tag="LUB18_15610"
     CDS             complement(7971..8351)
                     /locus_tag="LUB18_RS15610"
                     /old_locus_tag="LUB18_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_080490510.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(8793..8930)
                     /locus_tag="LUB18_RS15615"
                     /old_locus_tag="LUB18_15615"
     CDS             complement(8793..8930)
                     /locus_tag="LUB18_RS15615"
                     /old_locus_tag="LUB18_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            complement(9044..9664)
                     /locus_tag="LUB18_RS15620"
                     /old_locus_tag="LUB18_15620"
     CDS             complement(9044..9664)
                     /locus_tag="LUB18_RS15620"
                     /old_locus_tag="LUB18_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(9825..11003)
                     /locus_tag="LUB18_RS15625"
                     /old_locus_tag="LUB18_15625"
     CDS             complement(9825..11003)
                     /locus_tag="LUB18_RS15625"
                     /old_locus_tag="LUB18_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="WP_002324322.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDYE
                     KYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETV
                     IHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAIFMD
                     ATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDIYSRGVHDV
                     LLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRNIAHKVRVKDRKEICDDFKAVYQASS
                     KEEANTFLGSMIEKWQKTYPKVTQSLIKNQDLLTFYEFPPGIRRSIYSTNLIESFNKQI
                     KKYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE"
                     /name="IS256-like element ISEfm2 family transposase"
     BGC             1..7898
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4558..5294)
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7079..7989)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agttttataa ttcttctgta ttttataaac aaatttacta gcttttttca agtagttatt
       61 ttcttctacg gtgttgtttc tatttaattg attagtaata tttgttttat ataaattagt
      121 agttctttcc caatatttaa gagaattatt ttcggcttca ttatggatat ttattacttt
      181 tgtagaagct attaacagaa ggatcatgct taagaataga cagatattta atatacaaag
      241 atttaactta agtggtcgtg aattttttag ataatttttt atactagata aatgatagat
      301 acttagagta actatgataa ataaaaaact aattataaaa tgaaaaatac tacatagaag
      361 aaatgatagg aaaaaatttt cgttactgta atatatgctc aaaaaaatag ataaaatgaa
      421 agttataatc aaagaacaaa ataagaggtt taactcttta atagaatctt ttatagtatc
      481 aaaagagtta tagcctaaca ttttttttag agtgatttct ttcatttttg aaaaagcaaa
      541 tataattgta gtaattacaa taaatatact aagatagcag ataacaagaa caatattagt
      601 attaatttga aaagtatctg attgattgat attggaaata gtaggtgttc catatttttt
      661 cagtacagga attaatttgt ttgagtttcc ttgtacatat aatatttcac ttaatccaat
      721 attttccaca tggagagtgt aaaatatttt gtgtaaatag aaaaaaggaa gtcccttctg
      781 tagaatagag ttaccacaac acattcacag aaaagaggac ttccatatga acgattttac
      841 tacagaaatt ctaaagactc tagcgaacaa aggcgatttg aatgaattat tccgtgtcca
      901 tttggaaaaa gctgtcaata cgcttctcaa aacggagtta acggctttcc tcgattacga
      961 aaagtacgat cgcattggtt ttaacacggg taattctcgt aacggctcct atgaccgtac
     1021 ggtcaagacc gagtacgggg aacttcatct ccagattccg cgcgaccgca acggcgagtt
     1081 caagcaacag actgttcctg cttatagacg gacgaatgat accttagagg agaccgtcat
     1141 tcacctcttc cgaaaaggta ttaccatgtc ggaaatcgca gacttgattg agaaaatgta
     1201 tgggcatcac tacacgcccc aaaccatgtc caacatgact aaagtgctga ctgaagaagt
     1261 aaatgcattt aaagttagaa ctctaaatga taagtatgtc gctattttca tggatgctac
     1321 ttatattcct ttaaaacggc aaaccgtctc caaagaagca atctatattg ccattggtat
     1381 acgagaagac ggcactaaag aagtactaag ttatgcgatt gccccgactg aatcgaccta
     1441 cgtttggaat gagttgttac aggacattta ctccagaggg gttcatgatg tcttactgtt
     1501 tattaccgat ggcttaaaag gcatgaaaga tacgattcac cagatttatc ctaaagctaa
     1561 ataccaacat tgctgtgttc acgtttcccg taatattgct cataaagtac gtgtcaaaga
     1621 tcgaaaagaa atctgtgatg attttaaggc tgtttatcaa gctagctcaa aggaagaggc
     1681 aaataccttt ttagggagta tgattgagaa gtggcagaaa acttatccta aagtgacgca
     1741 gtcactgata aaaaatcaag atttattgac tttttatgag tttccgcctg gtatccgccg
     1801 aagcatttac tcaactaatt taatcgaatc tttcaataaa caaatcaaga aatacagccg
     1861 cagaaaagag cagtttcaaa atgaagaatc actagagcgt ttcctagtat ccatctttga
     1921 tacatacaat caaaaattct taaatagaag ccataaaggc ttccaacaag tgacggatac
     1981 attagcttca atgtttactg agtaacccat tattttgcag aaggacgagt tatttacaca
     2041 aaattattga cgctccctcc acatgagaaa aaggataaat atttatattt aaaaaattat
     2101 ttggaagttt taattggtac ttagaatttt tgttattagt ataatggact ttagaatcgt
     2161 ttcttgctaa tatatttttt ttagaaaatt tccagtgttt gttagcgtta gggtttgtag
     2221 caagaatagt tagttggtta acagcagtaa aactatattg agaaatatta atattttctt
     2281 gtgtagatat ttcatacaaa gcgtttatat aactgttaaa gtccatatca tctttatatt
     2341 gcatatgaat ttcagcggta tttttatata gcgcttcaga agtctttata ccatttataa
     2401 cattagtaga aaagaatagt attgtaatgc agacaaaaag tgataaaaca gcgtttattt
     2461 tttttaacat attttgaatt tcctttcaaa tgtaaataaa aaagagagtt taaactctct
     2521 tttttaataa acattccacc aaccagtatt tccagtaagt gtggcattgc atgttgcata
     2581 agctcttact ccagctttct gaactgatcg tacttgaccg cctcgtccat ttttagctgt
     2641 tgcactatgt ttagctctag catggtagta atttgcataa actttaaatc cattaacccc
     2701 acagttgaaa tctccgccgc cagcagcata tctcaattta ctagaagaaa atccttcttc
     2761 tgtagcttcc attcctcctc cgtaaatggc ggtatggtct tcgggtacat ctagatttaa
     2821 agcttctgta gcactaacta ttcctccact agttaataag acacccatta aagcagaaag
     2881 acataaaaat tttttcataa cttatttctc ctatcttttt tactagaatt attttatttt
     2941 agtgtgtttt ataaaataaa gaactttttt ggcgcgttag tatttttatg ataaaatttt
     3001 atgtaaataa tcgtttacat aggagaaaaa atggaggatt taggatctaa gctaagagaa
     3061 ttaagagtga aaaaagggta tactcaagaa tatgtggcaa gaaaatttaa tgttacacgt
     3121 caaactattt caaagtggga aaataacaaa acaacaccag atatagagac actaaaaaaa
     3181 atttgtgaat tttatagtac taacataaat tctattttaa atgaagataa tttaaaaaat
     3241 aaacaaaata atttactggt attgacagct attattttta atatatttgt tactatatta
     3301 ggtttatttg aattgattca aatgaatttt tttagcttta cacacggaat atttacttgg
     3361 tttgctctat ctggattaat agtaatagat atcttaaatt atttttatat ttactattct
     3421 tatttaaaaa atgaaaaatt tttttataca atattttttc ccctagtgat tttgattatt
     3481 ttattaagct aatatgaagt agttagttat ttaattgcat agttgacata atgtacacta
     3541 tgccaagtag cctctctacc aagggataca ggggctcact ttgtgatttt tcattggtct
     3601 aggtcaaaag ctctttttaa ggcttgattt tctgcagttg cttctttccg tttctttgtg
     3661 aatttttacc agtcattttt tactggattg ttatttttta taaaatatga taaaattttg
     3721 gtagcgcttt ataagcgcgt gctttttcaa gaataaacac tcattatttt ttaactggct
     3781 gctcggaaga gcagtctttt ttattattta tccgtctctt ttacaaacaa ttcttcgtta
     3841 ggactgttgt aataatttgg attcaatttc aattcaccat gtttattccc tttattgatt
     3901 actgacaata acatcggact gaagcctaga attcctagta aaatagaacc gaatagaaga
     3961 ttttttcttg tcatgaatgt tccctccagt tatttccaaa gttgccacca tttttgcttc
     4021 ttcttttttg tgtgtttttc tatcgattct gtttcttcag aaagaatagt agttgattca
     4081 tcttctggag tttcttccgt ataagctaat tgtaactgtt cttgcagttt ttcgatctgt
     4141 tgatttgcct gtagggtgag ctgttgttgc tggtcgagta atttttgcat attagaatta
     4201 ttttgctctt tgagggaaag ttgttctcgt aaaaattcat tttcttcacg caacttatct
     4261 agcaactcgt ttacaatcac gtttttgctt gatatataaa cgtttttatc gttttcttgt
     4321 tcatactcat gcaacctata tagtaacctt tggttaccta agttgctagc aagttgcaat
     4381 actccagaac gtaatacaac cttataacca cgctcatttt tagctaataa ggacgggtct
     4441 aatttatcaa catgtttaga gattgcttgc ctacttattt caaataattc agataattcc
     4501 tttattgtaa aggtttcttt gggttgcatg ataattgcct cctaaattgt taggtattat
     4561 ttctctaacc aattatctaa ggtaattgaa ggtatatttt cttcaaaata aatttgcaga
     4621 gatcctatct tatttccttt gtaggcttta atttttttga tttcaaaagt tttaaaaata
     4681 gcagggtgag ttgaattaac gtttaaaaat tcttttttcg cttgggcaaa aatttgttta
     4741 tcaatatctc tcatgcgata actttctggt attgctaata gagctctaaa atcttctatt
     4801 tttacaaacc agtgaccttt tttagatctt ctgtctttat gagccataag ttggcgatat
     4861 aactctttaa cgtagctact ctttaagttt gtcatatttt ctaattcgaa acgagtaaaa
     4921 ttagttccga ttgaattaag tatatattca aattcttcag aaacctttat taataatgtt
     4981 tcattcactt tatcgatttt aaatttttga aacagattta attgttcata tgttttttcg
     5041 tcttcataaa taaaagacaa agtaatgatt ttttttgaca tttttattaa atcattagca
     5101 aaacggtcgc cacgtgcatt tatattgtaa tcaattaatt cttttatttg tttaaatgta
     5161 aaaatttctt cttttgtacc ttttcttttc attttagaac atattcccca aaataaatcc
     5221 atatctatag gggtaaattt tcttaaaggg acagaattta atccattatt atatcgaact
     5281 atatcgttac tcaataaaaa tcacctcaat aaattatgtt atcatagaca taagaggatg
     5341 taaaacacaa tttatccccc atagtgtgac aatttatccc ccatagtgtg acaatttatc
     5401 ccccatagtg tgacaattta tcccccatag tgtgacaatt tatcccccac tacctatgtc
     5461 aatacttact ctcccataag gttgctaatc cctaacagtt attattaaca gtatatctaa
     5521 cagtatatct aacagtatat cttaagagca cattgaattt cccgcctaaa aaactcctac
     5581 tttttcctcc aaaaaggttc gcgaattttt ctaaactgga aaacttgctc cctacggtcg
     5641 ctaaaaaggt gtgatcgcta tgctctcata ttatactgcg cagtcgcctc cggctccttt
     5701 gctaaaaccg ttgttcaaag aggaggtgtg agactctgaa agcttgtcta agccgttcta
     5761 agacaattct tgattaattt cccttaatct agtcgagaag cgccttacag gcggtcaggg
     5821 caagccctaa caaccctaaa acaagtgata cttaaaaaca ccacacaaat ttaatttttt
     5881 aagatataat aacttgtcgc ctatccgaca gtcttgcgac tggaaggagg tgtatctaat
     5941 gtttgaactg ttcatttgcc cccttcttgt aggagtttgt gtctctttat tcagttattg
     6001 gttgaataac cgaaacaaga agtaaaatca ataaaggcga catcaacctg aaaaaaagcc
     6061 ccagagaatc cgacctctct ggggctttcg tgtatctaat gtttgatcat ttgcacattc
     6121 agtttaacat gattttaaat caaattcaat tttgttgttg ctgatctaac cattccttga
     6181 cactttttag atagtttaag gcggtttctt tttttgtcgg attttgcatg ttagttgctt
     6241 gttgaagtaa gatttcatag cgttcatatg tgacagattg ttgtttttct tttttttctt
     6301 cttcactggc aaaccaaggg tttgtgtctc caaatagatc actacaggct tcttctatct
     6361 ttgtatcttt tacatattgc acgtcttttt tggttcgctc atagttgtct tgaacctctt
     6421 tttgctgttt ttctggcaag ttttgataaa gacgagtgag tttttccatg tttttatccg
     6481 tcaaacgttt ttcttgattc acaaaaatat cgttcatttc ctcataacaa gtggtaaact
     6541 cttccatttt ttgtgctaat gcttgctctt ttttcttttt atcacgcgct gattcatact
     6601 tttgagtgac atttcctaat aaaatacttc tagaaacgcg cttatttccc tctaaaacgc
     6661 cgaataagcc gcttgaaacg attatcaagg aaaatataca gccaagaaag aaaaatcgct
     6721 taaatcgaat tttagagcct tttaatgaat atcttgtttg aaaaatttta tagatgagcc
     6781 aaatacttcc tgaaatacta acaaaaatga gtagataggg taaataaaat gccattttag
     6841 cctccccata ctaaccaaaa tgcgaacagt gtcaggacta agcaacataa gggggcaaga
     6901 aaatccaatg atcgttgaat aagcaccttt ttctgttcct tatttcgctg gtctaatagc
     6961 tcttgatgga gttttttaac ttgtaaatca atcgtagttt gtatcctttc ttgcgtataa
     7021 ggaacgagaa aagcacgttg tttctttatt tcgttttcta tgtcttttaa cgttttagag
     7081 cgataattct tgttggtggt ctgttcctct ttcaacgtgt tgttcatggt ttcttcgagc
     7141 cggtctaatt tccggttgtt caggcgtaag agttcgttgt tctcttcgta cttcttctgc
     7201 agtttcgcga atgctagctc gagctcttcg tagctcgttg gttcgctgtt ggttataggt
     7261 tcgttctcgt ctgtccagct catgtaaaat cgtctccttt tcgtagtcct ttcctagtct
     7321 gttggatcgt acttttttct ttttgtggat caattcgtaa gtgaaatttt tcccgcgatt
     7381 gaagcaatga atccccaatg ttttgaggga attacaaaat tggtcatagg ttctggtttt
     7441 tttgtttttc attgtttgat cgatcgcaat ccgaatttcg tctttccaaa caggttcatt
     7501 tttttctttt aattttattt cagacatggt tcgtttttct ggtttctttc ctcgctcagt
     7561 gattggtaag ttatgtgcaa gacaaatctc gtcatttagg tcataacatt tgtttagaaa
     7621 tgcttgacca tgagcttgaa atttttttcc agtctccaga ttaacggagt taatgacaat
     7681 gtgattatga atatggtctt tgtctgtatg tgtgtaaata gctgtttggt gatcgggggc
     7741 tatttttttt gcatactctg cccctagttg attagctttt tcagcggtaa tttctcctgg
     7801 tcgaaaagat tgaatcaata agtgtgcctg tacacggtcg tcttttccat acagcatgcg
     7861 aatttgtttc atttcacttt tggcataatc gacgtcacag tttattcctg ttttaatggt
     7921 tgctcgtggt tctgcgtaat tgattgctcg gctacaggaa gtggttcgac ttactttaac
     7981 tgttgccata tcttttgtag ctccttttgc atgattttta ggttttcgga taaacgttgg
     8041 gcgtctccag gagcaacgtc atgttggtgt gtattgcacc acttggcaat ttgattgata
     8101 ttccctccag ctttggctaa atcgcgcgcg attgcctggg caatgtctgg cgccactttc
     8161 ggtgtcacca gacgcgcgtt ttgtgccttg gctttcacga aagccggcac actcatttgc
     8221 aaagcttcag ccgattgctt cagctttgca aattcggatt cgctcactcg aaaactgatt
     8281 tgttttggct cggatcgata ggtatatttt ttagaagaat tgctagccaa attttgattt
     8341 tgttcgctca taagtcccac cctttcagta cgtcctacgg acgtatcgtt cgcaatacca
     8401 ttgtacctaa aaaggtacaa tgagtaaagc tcactaaaag ggtttggcaa gctctatatt
     8461 tgctagccaa atacctccgt atttggtgca aataaccgct tgtcaaaggg gcgcaagctc
     8521 ccccttgaat ccccctcaaa agccacgctc cacaatacaa aaaaagctag caaatgctag
     8581 ccactttttg taaagtttcg cttatttcac cgaagatgaa attgttgctt tgcaacaaaa
     8641 aacataaatg cttttttgtg taaactacta actatttagc taaaactgat agttttatta
     8701 aacattcagc aatattttta gccaattttt tatcagactt tcggcgagtc attatgataa
     8761 tatgtccact ttttgttact tctaccaaaa atctagcaat aactctccca ttcttcagtt
     8821 gatttagttt cataagtctt ttgtaattga cggtttaatt tttttggcaa ttgcaaagaa
     8881 taaataagtc tatcataaaa tttttcatac tcttttttca ataaaatcac ttgggtgcct
     8941 cctttttatt aagtgattat ttaaattttg ggataggaga gatggaatat aaaataaata
     9001 cccgtggtgc tagttttaaa tcagctagca tcacgggtaa aaattataat tctataatat
     9061 tagttccata gtttagaaca tttaagtcat gtgttactag tacaacggct tttttttctt
     9121 tttctgttaa atttttcaga aagttcatta taaaagttga gttttcttta tctaaatttc
     9181 ctgtaggttc atctgcaaat aatatatcaa atggttttaa catatttcta attaatgcta
     9241 ttctttgttg ttctccaccg cttaagagaa agattttttc ttttaagata tcttttggca
     9301 aagaaaaatc tgttaataaa ttataaatat gatcattgga aatattactg ttaaactttt
     9361 ttgctaattc gaaattgtat tgtactgttt gattttcaag aagagcatag ttttgaaata
     9421 aatagctaat atgatttctt cgtagacgtt gtatatcatg tttagaaact ttcttttcaa
     9481 aatagaataa gttaccggta tattgtttat ctattaggct taaattattt aacagagtag
     9541 tttttccact accacttttt cctgttacta taataaaatc tttttctgat attgaaaagt
     9601 tccaattttg tttgatcttt tttttattaa ttgacagggt gaaattctca actgataata
     9661 tcataaaata ttctccttaa aatatttaaa actatttttt ttataaataa taatagaagt
     9721 aattagattt attaaaaaga tcataaaaat tataaaatac aagtaagtgt ttgggagcgt
     9781 caataatttt gtgtaaataa ctcgtccttc tgcaaaataa tgggttactc agtaaacatt
     9841 gaagctaatg tatccgtcac ttgttggaag cctttatggc ttctatttaa gaatttttga
     9901 ttgtatgtat caaagatgga tactaggaaa cgctctagtg attcttcatt ttgaaactgc
     9961 tcttttctgc ggctgtattt cttgatttgt ttattgaaag attcgattaa attagttgag
    10021 taaatgcttc ggcggatacc aggcggaaac tcataaaaag tcaataaatc ttgatttttt
    10081 atcagtgact gcgtcacttt aggataagtt ttctgccact tctcaatcat actccctaaa
    10141 aaggtatttg cctcttcctt tgagctagct tgataaacag ccttaaaatc atcacagatt
    10201 tcttttcgat ctttgacacg tactttatga gcaatattac gggaaacgtg aacacagcaa
    10261 tgttggtatt tagctttagg ataaatctgg tgaatcgtat ctttcatgcc ttttaagcca
    10321 tcggtaataa acagtaagac atcatgaacc cctctggagt aaatgtcctg taacaactca
    10381 ttccaaacgt aggtcgattc agtcggggca atcgcataac ttagtacttc tttagtgccg
    10441 tcttctcgta taccaatggc aatatagatt gcttctttgg agacggtttg ccgttttaaa
    10501 ggaatataag tagcatccat gaaaatagcg acatacttat catttagagt tctaacttta
    10561 aatgcattta cttcttcagt cagcacttta gtcatgttgg acatggtttg gggcgtgtag
    10621 tgatgcccat acattttctc aatcaagtct gcgatttccg acatggtaat accttttcgg
    10681 aagaggtgaa tgacggtctc ctctaaggta tcattcgtcc gtctataagc aggaacagtc
    10741 tgttgcttga actcgccgtt gcggtcgcgc ggaatctgga gatgaagttc cccgtactcg
    10801 gtcttgaccg tacggtcata ggagccgtta cgagaattcc ccgtgttaaa accaatgcga
    10861 tcgtactttt cgtaatcgag gaaagccgtt aactccgttt tgagaagcgt attgacagct
    10921 ttttccaaat ggacacggaa taattcattc aaatcgcctt tgttcgctag agtctttaga
    10981 atttctgtag taaaatcgtt catatggaag tcctcttttc tgtgaatgtg ttgtggtaac
    11041 tctattctac agaagggact tccttttttc tatttacaca aaatatttta cactctcaag
    11101 tgttttttga aaatatcaat tgaaaacaca tagtagcaaa taactctatt gtaaaaggga
    11161 gtaaacataa atctaaaact tgacttattg gtgaatatcc aaatattttt tttaaagcta
    11221 ttttttttga atttatagta aaactaacaa aaatcatttg aaaattaatt attataaata
    11281 atcctaatat taaaagatat tgtagatatg catttaattt atttactttt ttttgagata
    11341 ataattcacc agtttcttga aaaacagaat aagaaaaatt tatgatattt tctaggttaa
    11401 attcttttat atacggatta atttgattaa aaaagttttg gtttgaagta tgtttctgat
    11461 caaaaaataa tccaccatca ctagtcaata tatttcccca aaataatgat tctgttattt
    11521 ctggattaat aactctaact atgggatcag ttattgtata gttatctttt gcctttccat
    11581 ataaggtatt aaaactaaaa tacttttggt tattattaac aaatattagt tttattttta
    11641 aatttttcaa attaggagtt ttataaggag cttgaggagg agagtcttca agtctaaact
    11701 gtatgtcttc taaataattt ttttcaatca tttttgcata ttctttatat tttttaggta
    11761 ctagaatgta agtagttagt ggatcagagt ttattaaatt taagtcagta gattcttcaa
    11821 aagaaatagg attgtaatat tttagatagt ttaaatcaac acatattcct gcaccagcag
    11881 gttgactaac atagtcttca aaatttggat aaactttttg tccaataaaa aattctttat
    11941 tattttcttg gatagttgcg taattgtatg gcgcgataat aaa
//
