LOCUS       NZ_CP089139             9988 bp    DNA     circular CON 21-FEB-2024
DEFINITION  Escherichia coli strain XD35 plasmid pXD35006, complete sequence.
ACCESSION   NZ_CP089139
VERSION     NZ_CP089139.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17183821
            Assembly: GCF_019375055.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9988)
  AUTHORS   Peng,Z.
  TITLE     Antimicrobial resistance and population genomics of multidrug drug
            resistant Escherichia coli in pig farms in mainland China: a
            national wide epidemiological and microbiological study
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9988)
  AUTHORS   Peng,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2021) College of Veterinary Medicine, Huazhong
            Agricultural University, NO.1 Shizishan Street, Wuhan, Hubei 430070,
            China
REFERENCE   3  (bases 1 to 9988)
  AUTHORS   Peng,Z., Chen,H. and Wang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JAN-2021) College of Veterinary Medicine, Huazhong
            Agricultural University, No. 1 Shizishan Street, Wuhan, Hubei
            430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. v1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1052.02x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/21/2024 03:28:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,403
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 4,970
            CDSs (with protein)               :: 4,970
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 314
            CDSs (without protein)            :: 314
            Pseudo Genes (ambiguous residues) :: 0 of 314
            Pseudo Genes (frameshifted)       :: 91 of 314
            Pseudo Genes (incomplete)         :: 226 of 314
            Pseudo Genes (internal stop)      :: 46 of 314
            Pseudo Genes (multiple problems)  :: 50 of 314
            Pseudo Genes (short protein)      :: 5 of 314
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP089139.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="XD35"
                     /db_xref="taxon:562"
                     /plasmid="pXD35006"
                     /geo_loc_name="China: Henan"
                     /collection_date="2019-10"
     gene            join(9381..9988,1..571)
                     /locus_tag="K0768_RS03285"
                     /old_locus_tag="LS330_03285"
                     /old_locus_tag="LS330_RS03285"
                     /pseudo=""
     CDS             join(9381..9988,1..571)
                     /locus_tag="K0768_RS03285"
                     /old_locus_tag="LS330_03285"
                     /old_locus_tag="LS330_RS03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612096.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(833..1966)
                     /locus_tag="K0768_RS03290"
                     /old_locus_tag="LS330_03290"
                     /old_locus_tag="LS330_RS03290"
     CDS             complement(833..1966)
                     /locus_tag="K0768_RS03290"
                     /old_locus_tag="LS330_03290"
                     /old_locus_tag="LS330_RS03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="WP_063084436.1"
                     /translation="MKISIIGNTANAMILFRLDLIKTLTKKGISVYAFATDYNDSSKEI
                     IKKAGAIPVDYNLSRSGINLANDLWNTYLLSKKLKKIKPDAILSFFSKPSIFGSLAGIF
                     SGVKNNNAMLEGLGFLFTEQPHGTPLKTKLLKNIQVLLYKIIFPHINSLILLNKDDYHD
                     LIDKYKIKLKSCHILGGIGLDMNNYCKSTPPTNEISFIFIARLLAEKGVNEFVAAAKKI
                     KKTHPNVEFIILGAIDKENPGGLSESDVDTLIKSGVISYPGFVSNVADWIEKSSVFVLP
                     SYYREGVPRSTQEAMAMGRPILTTNLPGCKETIIDGVNGYVVKKWSHEDLAEKMLKLIN
                     NPEKIISMGEESHKLARERFDANVNNVKLLKILGIPD"
                     /name="glycosyltransferase family 4 protein"
     gene            complement(1966..2775)
                     /locus_tag="K0768_RS03295"
                     /old_locus_tag="LS330_03295"
                     /old_locus_tag="LS330_RS03295"
     CDS             complement(1966..2775)
                     /locus_tag="K0768_RS03295"
                     /old_locus_tag="LS330_03295"
                     /old_locus_tag="LS330_RS03295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788525.1"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_000788525.1"
                     /translation="MKNHKVSIIIPCFNNEKTIERCVLSALKQTHRNIEIICINDGSSD
                     NSWNILKKLSSLYGNVFAFNNEDNSGPSFSRNKGVSLSTGHFLSFLDADDYWHPKKLEL
                     QLSFINDENLDFLGSTCSIGEKNNQEINQEIKKEHLKLKIISFNMMLFKNYFQTPAVIM
                     KRDIFIPFNENQRFSEDYMSWLVIVYNKKNKCGLIYGRDLVFLDKFNFGVSGLSGNLWL
                     MEKWELKNIFNFLLKGKIMAVPAILFSLIKYGRRCALTKKNKGKGNK"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(2772..3671)
                     /locus_tag="K0768_RS03300"
                     /old_locus_tag="LS330_03300"
                     /old_locus_tag="LS330_RS03300"
     CDS             complement(2772..3671)
                     /locus_tag="K0768_RS03300"
                     /old_locus_tag="LS330_03300"
                     /old_locus_tag="LS330_RS03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224710277.1"
                     /translation="MWFIFFFKRKPIHLSYLIALGLLFVSSFLLIKLFQLGFGDWGRLT
                     VPVFYDNSWSYFPLGYESSSDPNVLAFMLCIGSIYMFFTLPNKYTINIIFILSMMCSFL
                     TLSRSSFLSFFISFLIVSVYSFFVLKKTQLRLLALIMISLAMSFVVMKNIPAVVTENNG
                     ASFVTEPNITERIVSKASNSDRIARLKNAIVEISNLKTMFIGNGIGYSTLTQDPHNFYL
                     STIIDSGILILAIVMMVLTKPLLMVSRTPAKSNVILGCMFLTIYYMFISLFYWQVRVLY
                     FVIILLFTFVNISKKSEQ"
                     /name="hypothetical protein"
     gene            4059..5285
                     /locus_tag="K0768_RS03305"
                     /old_locus_tag="LS330_03305"
                     /old_locus_tag="LS330_RS03305"
     CDS             4059..5285
                     /locus_tag="K0768_RS03305"
                     /old_locus_tag="LS330_03305"
                     /old_locus_tag="LS330_RS03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080803.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="WP_063084438.1"
                     /translation="MNRLFSNAFWLMASQIGGLLIPLIELPLLARRLGADEYGKVVYVL
                     AISIMASVFIEYGFNFSAVKKIAGRKDDKYISQISTDVFLAKIILSIIPIILALTLIYT
                     TSSGISIPNNWFVWIILFILSFGFTPFWYYISIGNIIFPAILDLLLRVTGLILIVIFVN
                     SPSDAKTTIAIQSMVGALNTLISTAVVFYLSGVGAINIKRALNEIKDGFSVFLYKSSQG
                     IMITMSSSLLGFFGGAKSVGFFVPSEKVTKVITTLMITMLNVAYPHFVKNLSDSKNNIR
                     HIKKTILNISAILLFLSLIFSFFVFVFSDKIIFFVFGIEYERAIPLIKTIIWVIPLRSW
                     VLSLTILWFIPINKEKFITKITLVNIMLISFTACLFIYLFGTIGIVMSILLSEIITFVI
                     LTYFFFKEN"
                     /name="oligosaccharide flippase family protein"
     gene            complement(5487..6071)
                     /locus_tag="K0768_RS03310"
                     /old_locus_tag="LS330_03310"
                     /old_locus_tag="LS330_RS03310"
     CDS             complement(5487..6071)
                     /locus_tag="K0768_RS03310"
                     /old_locus_tag="LS330_03310"
                     /old_locus_tag="LS330_RS03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039052609.1"
                     /translation="MSDEIKKIAALIGHQQAQEKRVAALIEEFQAESAKLSRQTAQLAQ
                     VIHELDSASGKMTDTIRRSVNAALTQVEKELKQTGLAQQKPAVNALNQVVTAANESVSA
                     MRREMSRYTWKSAIYIALTIFAVMGGCLWGMYYFIDSGYDRIAKMQRMEAVWQKKAPLA
                     DISTCDGKPCVKVDTRQSYGDKKDTYLIIKQ"
                     /name="hypothetical protein"
     gene            complement(6084..7178)
                     /locus_tag="K0768_RS03315"
                     /old_locus_tag="LS330_03315"
                     /old_locus_tag="LS330_RS03315"
     CDS             complement(6084..7178)
                     /locus_tag="K0768_RS03315"
                     /old_locus_tag="LS330_03315"
                     /old_locus_tag="LS330_RS03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_153449126.1"
                     /translation="MASYHLSVKTGGKGKAASHADYIAREGKYAREKDNDLEHQESGNM
                     PAWAAHKPSEFWKAADTFERANGCTYREIEIALSREFTPAQRLELVRDFVQQEIGDRHA
                     YQFAIHNPRAAIEGGEQPHAHIMFSERLNDGIERDPQPYFKRANTKNPERGGAKKVRFG
                     ETPTERKAYLIAQRERWADLQNKHLERYQHIDRVDARSLKAQGIEREPERHFGAGQVRQ
                     FEPAQLHALMERREAERQAQHCRDECNSVIDVTTTLREALSERDNLTLKHEQTSEPQLK
                     ATSDKAFDFEKESDKLNALVSDAMKDIQEEIDLQTLVNDAMAEFQGIHQDMVKAEQQRQ
                     QEKERQRLAEQKRQKSDKGWSFSR"
                     /name="MobA/MobL family protein"
     gene            7172..7660
                     /locus_tag="K0768_RS03320"
                     /old_locus_tag="LS330_03320"
                     /old_locus_tag="LS330_RS03320"
     CDS             7172..7660
                     /locus_tag="K0768_RS03320"
                     /old_locus_tag="LS330_03320"
                     /old_locus_tag="LS330_RS03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_063084439.1"
                     /translation="MPCCFRPLQARTVSERQAKWLTDRVQTIRGLKSPNEPQRLLVILH
                     DKADKTAQEIKTLSLLIQAERAAEKAQEARTKVMNLIQAEKRAEAKAARKARDHALYQS
                     AGLLIMAGLVDSQTGKPVDDPAKLLGALVSLHELSRDNPKWEEWKLKGQVLLNQKKED"
                     /name="conjugal transfer protein TraD"
     gene            8259..9215
                     /locus_tag="K0768_RS03325"
                     /old_locus_tag="LS330_03325"
                     /old_locus_tag="LS330_RS03325"
     CDS             8259..9215
                     /locus_tag="K0768_RS03325"
                     /old_locus_tag="LS330_03325"
                     /old_locus_tag="LS330_RS03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001293214.1"
                     /translation="MSELVVFKANELAVSRYDLTEHETKLILFCVAKLNPTIENPTRDE
                     LTVKFSCSEYARTMGLSYENAWGRLNSATSDLFKRSVELIYPTGAVSKRIFNWTEYAEF
                     NREEQTVTLVFSSYIQPLLFHLKKFIKYNLEHVKAFENKYSMRIYEWLLKELSQRKTHR
                     GNIEISIKEFKFMLMLEKNYPLYAELNRWILKPVTNDLNTYSNMKLTIEKRGRPADTLI
                     FQVELDKQIDLVTELAKDPASKKEDKTIRLTPENRLHEGLKTTLHDALTAKIQLTSFEA
                     KFLSDMQSKYDLNGSFTWLTQKQRTTLEKILAKYGRI"
                     /name="replication initiation protein"
     gene            9223..9312
                     /locus_tag="K0768_RS27050"
     CDS             9223..9312
                     /locus_tag="K0768_RS27050"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_325914343.1"
                     /translation="MALISVTEAARLTGKNRATIHRYIKQGKL"
                     /name="helix-turn-helix domain-containing protein"
     Typing          8260..9215
                     /name="rep_cluster_1155"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.582"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6088..7178)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="85.714"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cctctaagga acacattgag atccctaatt atctggatcg ccagtttgct gttaccgagc
       61 ctaatcacgc ctggtgcggt gatgtgactt atatctggac gggaaaacgc cgggcttatc
      121 tggtgtagta ctcgatttgt tttcccgtaa accagctggc tgggcgatgt catttttccc
      181 tgattcagca ctgacagcta aagccctgtc gatggcctgg gaagcacggg gaaaaccgga
      241 taatttactg tatcactcgg atcaaggcag ccactatacc agcaggaatt tcagtcagtt
      301 actgtggaga tatcagataa agcaaagtct gagtcgccgg ggaaattgct gggataacag
      361 cccaatggaa cggttcttca gaagcctgaa aacagagtgg gtaccggata atggctacgc
      421 taattttagc caagccagca tggcaataac gaattacatc acaggatatt acagccagct
      481 cagacctcat caatataatg gtggtttgac gccgaatgaa tcagaacgat tgttcttgaa
      541 aaactctaaa gccgtggcca gtttttgttg accactacac aatgttgtat tttgcataca
      601 gcataatcga ctggaataaa attgtattgg aataggctct aacactgata taacggcagt
      661 ttagaaccta tctcaatagg gattaattac cgcataaaaa tatattatca ctgtaaccaa
      721 aacctcatac agcctgactt ttaaacatcg atatggctgg gtaaggtcat gaatttagct
      781 cctattgaga taggttctta gttccgaatg aatcagagcc gcttttcgtt tattaatcag
      841 gaattcctag tatttttaat aactttacat tatttacatt tgcatcgaat ctttctcttg
      901 ctaacttatg actttcttct cccatactga ttattttttc aggattatta attaacttca
      961 gcattttttc tgcaagatct tcatgtgacc atttttttac aacatatcca ttcacaccat
     1021 caataattgt ttctttgcag cctggtaaat tagtagttaa aatcggtctc cccatagcca
     1081 tcgcttcttg tgtactacga ggaactccct ctcgataata ggaaggaaga acaaatacgc
     1141 ttgatttttc aatccaatca gccacattag aaacaaatcc gggataagaa ataactcctg
     1201 atttaattaa agtatctacg tcagattcag ataaccctcc ggggttttcc ttatctatag
     1261 cgccaagtat aataaattca acattgggat gtgttttttt tatttttttt gcggcagcaa
     1321 caaactcatt gactcctttt tctgctagca aacgagctat aaaaatgaat gatatttcat
     1381 ttgttggtgg cgtgctttta cagtaattat tcatatctaa accaatgcca ccaagaatat
     1441 ggcaagattt taattttatt ttgtatttat ctatcaagtc atgataatca tccttgttaa
     1501 gaagtattaa tgagttgata tgtggaaata ttattttata caggagaacc tggatatttt
     1561 taagtaactt tgtttttaac ggagttccat gtggctgctc tgtaaataaa aaacctaacc
     1621 cctcaagcat agcgttatta tttttaacgc ctgaaaaaat accagccaac gatccaaaga
     1681 tagagggctt tgaaaaaaaa gataaaatag catctggttt tatcttcttt agttttttac
     1741 ttaataagta agtattccat aaatcattag caaggttaat accactgcga cttaaattat
     1801 aatcaacagg aatggcgcct gcttttttta ttatttcctt ggatgaatca ttatagtcag
     1861 tagcaaaagc atagactgaa atccctttct tggttagtgt ttttattaaa tccaatctaa
     1921 acaaaatcat agcatttgct gtgttcccta ttattgagat cttcattatt tattaccctt
     1981 acctttattt ttctttgtta aagcgcatct tcttccatat tttatcaaag aaaacaagat
     2041 cgcaggcact gccattattt tacctttcaa caagaagtta aatatatttt ttaactccca
     2101 cttctccatc aaccataaat taccactcaa ccctgacact ccaaagttaa atttatcgag
     2161 aaaaaccaaa tcccttccat atattaatcc acatttgttt tttttattat aaacgataac
     2221 aagccatgac atgtagtcct ctgaaaaacg ctgattctca ttaaatggaa taaaaatatc
     2281 tcttttcata atgacggctg gagtctggaa ataattctta aacaacatca tgttaaatga
     2341 aattattttt aattttaaat gttctttttt aatttcttgg ttaatttctt ggttattttt
     2401 ctcaccaatg gaacacgttg aacctaaaaa atccaagttt tcatcattaa taaatgatag
     2461 ttgtaattct agtttttttg gatgccagta atcatctgca tccaaaaatg acaaaaaatg
     2521 ccccgtggat agtgaaactc ctttattcct ggaaaatgac ggccctgaat tatcctcatt
     2581 attaaaagca aaaacatttc catacaatga gcttaatttc tttaaaatat tccagctatt
     2641 atctgacgat ccatcattta tacaaataat ctctatattt ctatgggttt gttttaatgc
     2701 ggaaagaacg catctttcta tagttttttc gttattaaaa caggggatga taatactaac
     2761 cttatggttt ttcattgctc gctcttctta cttatattta caaaggtaaa taataatata
     2821 ataacaaagt ataggactct cacttgccag tagaaaagag aaatgaacat atagtatata
     2881 gtcaagaaca tacaccccaa aataacattt gattttgcgg gggttcttga aaccattaat
     2941 aatggttttg ttagcaccat cattacaatt gctaatatca aaataccgga atcaataata
     3001 gttgataagt aaaaattatg tggatcctgt gttaatgtac tatagccaat accattacca
     3061 ataaacattg tctttagatt acttatttca acaatagcat tttttaatct ggctattcta
     3121 tcactattac ttgctttact aactattcgt tctgttatat ttggttctgt gacaaatgaa
     3181 gctccattat tctcagtcac tacagcggga atgtttttca tcactacaaa agacattgca
     3241 agtgaaatca taattagtgc caacaacctt agctgtgttt tctttagcac aaagaatgaa
     3301 taaacactta caataagaaa ggaaataaaa aaagataaaa aagaactccg tgataaggtc
     3361 aaaaatgaac acatcattga caatatgaat ataatattaa ttgtgtattt atttggcaat
     3421 gtgaaaaaca tatagattga gccaatgcat aacataaaag ctaacacatt tgggtcactt
     3481 gaactttcat aacccaaagg aaaataagac catgagttat cataaaaaac aggaaccgtt
     3541 aaacgtcccc agtcaccaaa gccaagttga aataatttaa tcaacaaaaa agagctaaca
     3601 aaaagcaggc ctaaagcaat taaatatgat aaatgtattg gttttctttt aaagaagaaa
     3661 ataaaccaca tagatattac tatatataca gcatgactag taattagagt gatagattta
     3721 tgattatata caaaaatgtt aatgacaagg taagcaacta ctaagcatag gagaaaaata
     3781 tgtgatcttt ttataaaaga ctttcttttt ataagccaat agcaacctaa tagtgtacag
     3841 gaaaagagag tgtatactgt aaccacatga tcactattga ctcccatgac cacctgttta
     3901 ttccctccca taactgacca caatataaaa ggaaaagtgc aaatgtatag ataaaaacac
     3961 atagtgattc aactggttat ttttattgta aataagattc ttctataata tcataaacat
     4021 gatgcctcac tcaacatttt taacagatga tattgtttat gaacagactt ttttctaatg
     4081 ctttctggtt aatggcatct cagattggcg gccttctaat ccccttgatc gaactgccat
     4141 tgcttgccag aagactgggg gctgatgaat atggaaaagt tgtatatgtt ttagctattt
     4201 ccataatggc ttctgttttc attgagtatg gattcaattt cagtgctgtg aaaaaaatcg
     4261 caggaaggaa agatgataaa tatatttcac aaatttcaac agatgttttt cttgctaaga
     4321 taattcttag tattatacct attattttag ctctcaccct aatatacaca acatctagcg
     4381 gaatatctat acctaataat tggtttgtat ggattatttt atttatctta tcttttggat
     4441 tcactccgtt ctggtattac atatcaatag gaaatataat tttccccgca atactcgatc
     4501 ttttactaag agtgacaggt ttaattctca ttgttatttt tgttaactcc ccgagtgacg
     4561 caaaaaccac aatagccatt caatcaatgg taggagcgct aaatacatta atatcaactg
     4621 ctgttgtttt ttatttgtcg ggagtaggag caattaatat taaaagagcg cttaatgaaa
     4681 ttaaagatgg gtttagtgta tttttgtata aaagttcaca aggaataatg ataaccatgt
     4741 catcatcatt attaggtttt tttggggggg ctaaatctgt tggttttttt gttccatctg
     4801 aaaaagtaac taaggttata acaacattaa tgataacaat gctaaacgtg gcatatccgc
     4861 atttcgttaa aaatcttagt gatagcaaaa acaatatacg ccacataaaa aaaacaatcc
     4921 ttaacatatc agcaatatta ttatttttat cattaatttt ttcttttttt gtttttgttt
     4981 tctccgataa aataattttt tttgtttttg gtattgaata cgaaagagcg ataccattaa
     5041 ttaagactat tatttgggtt ataccgttaa ggtcatgggt gttgtcactt acaattctat
     5101 ggttcattcc aataaataaa gagaaattta tcacaaaaat aacactcgtt aatataatgc
     5161 taatttcgtt cactgcgtgt ttatttattt atctgtttgg tactatagga atagtgatgt
     5221 caattttact atcagaaata ataacttttg tgatattgac ctatttcttt ttcaaagaaa
     5281 actaagagcc tatcccaaaa ggaattatcg tataatataa tgaattacga taatttctga
     5341 gggcaacgta tggcaggcaa caagtggcaa atcagtgata gactctggga gaaaatggct
     5401 cctctaatcc taaccatgaa ccgctcaaaa ccacctcgct actttgacga cttgcacggg
     5461 aaaacggcgt attcagcgcc gtttttttat tgtttgataa tcagataagt gtctttttta
     5521 tcaccatatg actgccttgt atccactttc acacaaggtt ttccgtcaca cgtggagata
     5581 tcagccagcg gggctttttt ttgccagacc gcttccatgc gctgcatttt tgcaattcgg
     5641 tcataaccgc tgtcgataaa ataatacatt ccccaaagac acccgcccat caccgcaaat
     5701 atcgtcaatg cgatataaat ggcagacttc cacgtataac gggacatttc acgtcgcatt
     5761 gcactcacgg attcattagc tgctgtaacc acctgattca gcgcattgac ggcgggtttc
     5821 tgctgcgcca atcccgtttg tttgagttct ttttcaacct gagttaaggc tgcattcacc
     5881 gatcgcctga tcgtgtcggt catcttaccg gaggcactgt ccaattcatg gatcacctga
     5941 gccaattgtg ctgtttggcg gcttaatttg gcggattccg cctgaaattc ttcaatcaac
     6001 gccgccacac gcttttcctg cgcttgctga tgcccaatca gcgccgcaat ttttttaatc
     6061 tcatcactca tacaaaatcc tgttcatcgg gaaaatgacc agcctttatc ggacttttgt
     6121 cgtttctgct cggcgagtcg ctgccgttct ttttcctgct gacgctgttg ctcagcctta
     6181 accatgtcct gatgaatgcc ctgaaactcc gccatcgcat cgttaacaag ggtctgaaga
     6241 tcgatttctt cctgtatgtc cttcatggca tcactgacca gtgcgttcaa cttgtcagac
     6301 tctttttcaa aatcaaacgc tttgtctgaa gtcgctttca actgaggctc tgacgtctgc
     6361 tcgtgtttca gtgtcagatt atcacgctcg ctgagggctt cccgtagcgt ggtggtaaca
     6421 tcaatcacgc tgttacattc atcacgacaa tgctgagcct gtcgctccgc ttctctgcgc
     6481 tccatcaggg cgtgtaactg cgcgggttca aactgcctga cctgtcctgc gccaaaatgt
     6541 cgctccggct cacgctcaat cccctgtgct ttcagggaac gcgcatcaac gcggtcgata
     6601 tgctgatacc gttcaagatg cttattctgt aagtcagccc agcgttcccg ctgggcgata
     6661 aggtatgcct tgcgttcggt cggggtttcg ccgaaccgca ccttttttgc gccgccccgt
     6721 tcagggtttt tcgtgttagc ccgtttaaaa tagggctgcg ggtcacgctc gataccgtca
     6781 ttaagccgtt cagaaaacat aatatgcgcg tgcggttgtt caccgccttc aatcgccgct
     6841 ctggggttat gaatggcaaa ttgataagcg tgccggtcgc caatttcctg ttgaacaaaa
     6901 tcgcgaacca gttcaaggcg ttgtgcgggt gtaaattcac gcgacagggc aatttcaatt
     6961 tcgcggtagg tacagccgtt ggcacgttca aacgtgtcag cggctttcca aaactcggac
     7021 ggtttatgcg ctgcccatgc gggcatatta ccggactctt gatgctcaag gtcattatct
     7081 ttttcacggg catattttcc ctcacgcgca atataatcgg catgggaagc ggctttgcct
     7141 ttcccgccgg ttttaacgct gagatgataa gatgccatgc tgttttcgcc cgctacaagc
     7201 gcgcaccgtt tctgaacggc aagcaaaatg gctcacagac agagtgcaaa ctatccgtgg
     7261 tttaaaatca ccgaatgagc cacaacgctt attggtgatt ttgcacgata aagcggataa
     7321 aaccgcccaa gaaataaaaa cgctctcatt gttaattcag gcggagcggg cagcagaaaa
     7381 agcccaggaa gcccgcacaa aggtaatgaa tcttattcaa gcagagaagc gtgcagaagc
     7441 caaagctgcc cgtaaagcac gtgaccatgc cctttatcaa tcagcggggt tactcatcat
     7501 ggcggggtta gtggacagtc aaacgggtaa accggtagat gaccccgcaa aactactggg
     7561 tgcattagtc agcttgcatg aattatcccg tgataatccc aaatgggaag aatggaaatt
     7621 gaaggggcag gtattactca atcagaaaaa ggaagattag agcacagtgt taataccctc
     7681 taaaacgcca cagaaagtcc gtggcgaggt tttgttggtt tgataatgga atttatcacc
     7741 taatactgaa aacatggcac agaagcgcaa acaaccacct tcctgattga gccgtgagcg
     7801 aagcgaaccc aatgacacga aaaaaacagc ggcgctgtgg ctggatggtc ggagacaaaa
     7861 gaaaggggca gcgcgttgcc cgagcaaagc gagggagcta cttaggtgtt ttaggtttgt
     7921 cttgaaaagg agcaaacgtt gtttgcgaca tccttttaac tacggcaaat cgacggcaat
     7981 tttctgattt atccacaggg ctgggatcta ttcttttttt atcttttatt tattctttct
     8041 ttattctaat aattttaact tattgttttt taataaaaaa aaaagcatta aatgtaggaa
     8101 aaattaggct agatgtagga aaaattaggc tagcagtggg aaagatgtag gaaaaattag
     8161 gttattcaca atcaaacccg aaaaaatgta gttgatttaa atcgctacat cattatgatt
     8221 catgtagcga tttaaattac tacataatgg tgattagcat gtctgaatta gttgtgttta
     8281 aagcaaatga attagcagta agccgttatg atctaactga acatgaaacc aagctaattc
     8341 tgttttgcgt tgcaaagttg aaccctacaa ttgaaaaccc aacaagggat gaattaacag
     8401 ttaaattctc atgttctgaa tatgcacgca ctatgggctt aagttatgaa aatgcttggg
     8461 gaagattgaa cagtgcaact agtgatttat ttaaacgctc tgttgaatta atttatccta
     8521 caggagcagt ttctaaacga attttcaatt ggacggaata cgctgagttc aatagagaag
     8581 aacaaactgt aacgttagta tttagctcat atattcaacc acttttattt catttaaaaa
     8641 aattcattaa atacaacctt gagcatgtta aagcctttga aaataaatac tcaatgcgaa
     8701 tatatgaatg gttactaaag gagctttcac aaagaaaaac gcatagggga aacattgaga
     8761 taagtatcaa ggaatttaag tttatgctca tgcttgaaaa aaactaccct ttatatgcag
     8821 aattgaatcg ctggatccta aaaccagtta caaatgactt aaacacttac agcaatatga
     8881 agttgactat tgaaaaacgc ggtcgtcctg ctgacacact aatctttcaa gttgaactgg
     8941 ataaacaaat tgaccttgtg actgaactag caaaagatcc cgcatcaaaa aaagaagata
     9001 agacaatccg tttaacgcct gaaaatcgtc ttcatgaggg gctaaaaaca acattgcatg
     9061 atgctttaac tgcaaaaatt caactgacta gttttgaagc aaaattcctg agcgatatgc
     9121 aaagcaagta cgatcttaac ggctcattta catggctaac tcaaaaacaa cgaactacgc
     9181 tagagaaaat tctggctaaa tacgggcgga tatgaggtat ctatggcact gatatcagtt
     9241 acagaggcag ccagactaac tggaaaaaac agagcaacaa tacatcgcta tattaagcaa
     9301 ggaaaactgt agtggcatac tgaatttggc cacctgaata gaggtgatat catcacttcg
     9361 cagtaaaaac aggtgacatt atgaccggac gtaacagacg caattttagc cccgggtttc
     9421 gcctcgaagc tgcccagctt ttactcgatc agcattacac cgttgccgcc gctgctacgg
     9481 caatgaacgt cggcaaatcc acaatggaca aatgggttcg acaactgaaa ggagagcgag
     9541 cgggaaaatc ccccactact tcacccatca cctgagcaga ttgaaatacg cgagctaaag
     9601 aaaagacttc aacgtgttga aatggaaagg gatatattaa aaaatgctac cgagaccttg
     9661 atgtcagact ccctaaacaa ttctcattag ttgagaaact cagggcgcag tttcctgttg
     9721 ccattgtgtg caacgtgttt ggggttcatc gcagcagtta taaatactgg cggcagcacg
     9781 ggaagcctga cgccacgaga gtggcattac tgagccttgt gcgtgaagtt tatcgcgaaa
     9841 gtaacggctc agcaggagcg cgaagcatta ccgcgatggt caccaccaaa ggtataaaac
     9901 tgagccgctg acgggcaaca aagctaatga aagcgctcaa tattatcagc tgtcagcaac
     9961 ctgtccatcg ttataagaag gcgtctaa
//
