LOCUS       NZ_CP089025             3511 bp    DNA     circular CON 12-FEB-2024
DEFINITION  Klebsiella pneumoniae strain 49210 plasmid p49210-3.511, complete
            sequence.
ACCESSION   NZ_CP089025
VERSION     NZ_CP089025.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15146843
            Assembly: GCF_021166235.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Chen,L., Hao,M., Lin,Y. and Kreiswirth,B.
  TITLE     Whole genome sequencing of K. pneumoniae isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Chen,L., Hao,M., Lin,Y. and Kreiswirth,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 111 Ideation Way, Nutley, NJ 07110,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-SEP-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/12/2024 03:27:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,958
            CDSs (total)                      :: 5,835
            Genes (coding)                    :: 5,637
            CDSs (with protein)               :: 5,637
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 78 of 198
            Pseudo Genes (incomplete)         :: 121 of 198
            Pseudo Genes (internal stop)      :: 36 of 198
            Pseudo Genes (multiple problems)  :: 34 of 198
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP089025.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="49210"
                     /db_xref="taxon:573"
                     /plasmid="p49210-3.511"
                     /collected_by="HMH-CDI"
     gene            345..527
                     /locus_tag="LRY78_RS26600"
                     /old_locus_tag="LRY78_26590"
     CDS             345..527
                     /locus_tag="LRY78_RS26600"
                     /old_locus_tag="LRY78_26590"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016265984.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     gene            1723..2064
                     /locus_tag="LRY78_RS26605"
                     /old_locus_tag="LRY78_26595"
     CDS             1723..2064
                     /locus_tag="LRY78_RS26605"
                     /old_locus_tag="LRY78_26595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2273..>2701
                     /locus_tag="LRY78_RS26610"
                     /old_locus_tag="LRY78_26600"
                     /pseudo=""
     CDS             2273..>2701
                     /locus_tag="LRY78_RS26610"
                     /old_locus_tag="LRY78_26600"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2742..3239
                     /locus_tag="LRY78_RS26615"
                     /old_locus_tag="LRY78_26605"
     CDS             2742..3239
                     /locus_tag="LRY78_RS26615"
                     /old_locus_tag="LRY78_26605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            3246..3461
                     /locus_tag="LRY78_RS26620"
                     /old_locus_tag="LRY78_26610"
     CDS             3246..3461
                     /locus_tag="LRY78_RS26620"
                     /old_locus_tag="LRY78_26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1465..1638
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(977..1498)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.69"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2055..3511
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 aagacagcag atctggtacc ggaacggcaa cgcacattat ctcatggtga cagggagtat
       61 ggagactgat aaacgcccgt ctcaggtggc ctgagcgtga cggggatcat ggccattttc
      121 tgcgatttca gcacacagag agtctttttt aaccgaaatc accggctatg cagtgatgtg
      181 aataagtgat gcatatgcgt aaaataaatc aggtgcgttt ctggtggttt tcggtgtact
      241 tgccaccacc ttttgcccgt cagccatcag aaacgccctg agtgcctccg gcgcggtgcg
      301 ggtaagagat gttatgttta atgttgataa caggagtaag agtgatgctt acatttattg
      361 gtgtagtggt agtcgccgtt attgcattta aattatttat gctttttttt agttcatccg
      421 aatctgccgt cgaacgtgct acccgtaaat atatggagaa ccctacgagt gctaactata
      481 aaataatggt ggctacacgc atgagaaatg agaagaaaag agggtgatat atgtttgagt
      541 ttattggttt tttattttta cttttggtgt gttatgtctt tatacaaatg gttgctgtca
      601 gggtgtttcc tgaatatgga gtacggaaag cggaggaacg ataccgtcga aatccatcag
      661 cagaaaatga ggatctgtta tggagagcta gaagtcgtat aactaaacgc aagtaatgtt
      721 tcctaagtgt gttgataggc aaacatggtg ggagttacta ctctcggagt taatgatctt
      781 cttgtgatcc tgtcgtttca gtcgtaatct cttgctctga aaacgaaaaa accacctggg
      841 gaggtggttt gatcgaaggt taagtcagtt ggggaactgc ttaaccaggt aactgggtat
      901 acgagaccgc agataccaaa actgttcttt cagtgtagcc gcagtcaggc ccatacttca
      961 agacctctga atctctctct cgtatatccc agttaccagt ggctgctgcc agtggcgctt
     1021 tgtcgtgctt tctcgggttg gactcaagat gatagttacc gggtaaggcg cagcagtcgg
     1081 actgaacggg gggtttgtgc atacagtcca gcttggagcg aactgcctac ccggaactga
     1141 gtgtcaggcg tggaatgaga taaacgcggc cataacagcg gaatgacacc ggtaaaccga
     1201 aaggcaggaa caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat
     1261 agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg
     1321 gggcggagcc tatggaaaaa cggcttcgcc gcggccctat cgcttccctg ttaagtatct
     1381 tcctggcatc ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc
     1441 gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat
     1501 tctgctgacg cgccggtgcc gccttttttc tcctgccaca tgaagcactt cactgacatc
     1561 ctcatccgtg ccaacatagt aagccagtat acactccgct agcgctacgt gactggttca
     1621 gggctgcgcc ccgaaacccc actgacgcgc ctgcggcttg tccaaccccg cgccgaccgg
     1681 gaaaggcttt cccgtccgtc acggcgttat caggaggcga ttctggcaga taaacgcaac
     1741 aagatgctca cgatgtgggt gacggaagac gaacaccggc ggttgcttga gcggtgcgac
     1801 ggaaaacaac tggcggcgtg gatgcgccag acctgccttg atgagaaacc gacacgcgcc
     1861 ggcaggcttc cctcgatttc gcccgcgctg ctgcgtcagc ttgccggcat gggcaataac
     1921 ctgaaccaga tcgcccggaa agtgaacgcc ggtggtgccg gtcatgaccg ggtgcagatc
     1981 gtggcagcgc tgatggcgat cgatgccggt ctggaacggt tgcggcatgc cgtactggaa
     2041 aaagggccgg acgatgattg ttaaattcca tccccggggt cgcggcgggg gcgccggtcc
     2101 tgtggattat cttctgggta aagaccggca gcgtgaaggt gccagcgtcc tgcaggggaa
     2161 gccggaagaa gtccgggagc tgattgatgc gtcgccgtat gtgaaaaaat atacctccgg
     2221 ggtgctgtca ttcgcggagg cggacctgcc gccgggtcag cgtgaaaaac tgatggccag
     2281 ctttgagcgg gttctgatgc ccggactcga taaagaccag tacagcattc tgtgggtgga
     2341 acacgcagac aagggtcgtc ttgagctgaa ttttctgatc cccaataccg aattactgac
     2401 ggggaaacgc ctccagccgt attacgatcg tgccgaccgg ccgcgcatcg atgcgtggca
     2461 gacagtggtc aacggcaggc tggggctaca tgatccgaac gacccggaga accggcgggc
     2521 gctggtcacg ccttcaggac tgccagaaac gaagcaggaa gccgcagaga cgattacacg
     2581 gggtttgctg atgttagcct catcaggaga gctgaaaacg cgtgatgacg tcacaggggc
     2641 gctcacagcg gccggttttg aggtggtcag aaccacaaaa aacagtatca gcattgccga
     2701 cccggacggg gggcgaaata tccgactgag gggagccatt tatgagcagt cttttaacgg
     2761 cctcgaggga cttcgaggag aaatcgaaag cacagcagca gcttaccgaa gagaggctga
     2821 aagccgcatt cagcaagcac gagaacgttg tcaaatcgga actgaacgca agcgcgaaga
     2881 gaatcaacga cgccatcagc gcccacgaac aggggatgaa cgcggcgatg cagtcgaacc
     2941 ggctgagcgt gctgcgaatg gtcgggcgga cgtggctgac catcaccatg gtgtcagggc
     3001 tgctgttcgc cagcctgagc ggggtgctgt ggtaccaggg gaaccggata gcggcgaacc
     3061 tggcggaaat caggcagcag agagatacgc tgtcgaaact gcacatgcag acgtggggcg
     3121 tgacctatct ggagaaccgc aacggacggt ttctggtgct gccggagggg atgaaagccg
     3181 aaacgggccg gacggtggac aacaagacgc ggaacgcggt gaagctggta cgggagtaga
     3241 gcagaatgac ggaactggaa aaacaattgc tgagcgcgtt agagcagcta cagcaggact
     3301 actcgagaag gctggacgag tgggagagcg ccttcgagga atggcggaag atgtctggtc
     3361 ttatgcaacg ggagaacgcg gcgctgagcg agcgcgtgtc gggcttgagc aggcaggtgc
     3421 agagtttgag cgagcagctg cgccggttat cgacaggctg aatgagattg agcaacagcg
     3481 atacgggcaa acaacggaaa aggaaccaga g
//
