LOCUS       NZ_CP089006             7508 bp    DNA     circular CON 12-FEB-2024
DEFINITION  Klebsiella pneumoniae strain 56661 plasmid p56661-7.5, complete
            sequence.
ACCESSION   NZ_CP089006
VERSION     NZ_CP089006.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23552368
            Assembly: GCF_021166135.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Chen,L., Hao,M., Lin,Y. and Kreiswirth,B.
  TITLE     Whole genome sequencing of K. pneumoniae isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7508)
  AUTHORS   Chen,L., Hao,M., Lin,Y. and Kreiswirth,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 111 Ideation Way, Nutley, NJ 07110,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-SEP-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/12/2024 03:45:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,968
            CDSs (total)                      :: 5,844
            Genes (coding)                    :: 5,674
            CDSs (with protein)               :: 5,674
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 70 of 170
            Pseudo Genes (incomplete)         :: 104 of 170
            Pseudo Genes (internal stop)      :: 30 of 170
            Pseudo Genes (multiple problems)  :: 31 of 170
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP089006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="56661"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p56661-7.5"
                     /geo_loc_name="Russia"
     gene            complement(join(6855..7508,1..750))
                     /locus_tag="LRZ77_RS26440"
                     /old_locus_tag="LRZ77_26440"
     CDS             complement(join(6855..7508,1..750))
                     /locus_tag="LRZ77_RS26440"
                     /old_locus_tag="LRZ77_26440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY-like element ISKpn21 family transposase"
                     /protein_id="WP_001567368.1"
                     /translation="MSAESSGVFTLKEINRIKIIQDVIERRITTRRAAEHLGISDRQCR
                     RLLARYREGGPLGMASRRCGMRGNRQLPPGLADQALELIKTRYADFGPTLAREKLEELH
                     GLFLGKETVRRIMVRAGLWVPRKQRAARIPQPRYRRPCTGELIQIDGCDHDWFEGRGPA
                     CTALVYVDDATSKLMELLFVKSESTFSYFEATRRYIDKHGKPLALYSDKAGVFRVNNKH
                     ATGGDGHTQFGRAMHELNIQTICAETSPAKGRVERAHLTLQDRLVKELRLQGICSMEAA
                     NDFAEAYMADYNRRFGKVPRHDFDVHRAVEHDEDLGLIFTVREKRKVSKSLTIQYDKML
                     YLIEDSELSRRAIGKYIDVYHYPDGRKELRLNGTLLPYSTYDRLSEIDQGAIVDNKRLG
                     RTLEFISLVQSKRDNTRSQSIPAGDGPSRRRPKQEGKKSQRSLDNDDMLEALKQLQSRS
                     EDIFGKRAR"
                     /name="ISNCY-like element ISKpn21 family transposase"
     gene            960..2210
                     /locus_tag="LRZ77_RS26445"
                     /old_locus_tag="LRZ77_26445"
     CDS             960..2210
                     /locus_tag="LRZ77_RS26445"
                     /old_locus_tag="LRZ77_26445"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160339.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001584442.1"
                     /translation="MTRPIAVDLFAGAGGLSLGFEQAGFDIMASVEIDPIHCSAHKYNF
                     PMCKTICRSVVDVTGEYVREVSGIGEKEIDVVIGGAPCQGFSLIGKRALDDDRNKLVYH
                     YVRLVLELKPKYFVFENVKGLTVGKHKQFLVEIMDAFKDGGYDVVSDYKVLNAADYGVP
                     QDRRRLFLMGGRKGIALPSYPEPLSIRTTVGDAIGDIPDAEKYPELWGRDWVYAKFGEQ
                     SLYSSYLHGVGKDPSDFSYPRIFNKDKLTCSLLTDHSDISRGRFVETLPDTVEPISRFK
                     KLSMNGLCNTLRAGTASDRGAFTSPRPIHPIYPRVITVREAARLHSYPDWFRFHVTKWH
                     GFRQVGNSVPPMLARSVGLQIMKVLGVTPKKPTKKIKLGDESLVAISMSDAASMFGVSS
                     NVIAKRIKNSNKEVVCE"
                     /name="DNA cytosine methyltransferase"
     gene            2203..3012
                     /locus_tag="LRZ77_RS26450"
                     /old_locus_tag="LRZ77_26450"
     CDS             2203..3012
                     /locus_tag="LRZ77_RS26450"
                     /old_locus_tag="LRZ77_26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032446875.1"
                     /translation="MSKDKKERSKNRYHQLVEKIFFDKYVDGDLEVLFERLDINSAAKD
                     LGIELPSNVGDVIYSIRFRTPMPESILLTQPEGKEWIIELAGRSKYRFRLVTISRILPN
                     PNMVAIKIPDNTPEIITGYSLDDEQALLAKVRYNRLIDIFLGITTYSLQNHLRTTVKGI
                     GQIEIDELYVGIDKYGCHYIIPVQAKGGTDQISMVQTSQDTAWCEQKYPNVRCRAISAQ
                     FVSSDLIALFELKIDEGQVKLIEERHYKLVPVKELDSSDISNYRMSV"
                     /name="hypothetical protein"
     gene            complement(3232..3363)
                     /locus_tag="LRZ77_RS26455"
                     /old_locus_tag="LRZ77_26455"
     CDS             complement(3232..3363)
                     /locus_tag="LRZ77_RS26455"
                     /old_locus_tag="LRZ77_26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072007956.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPKRHAKAPLAAATGNRIHGAE"
                     /name="replication initiation protein"
     gene            3995..4336
                     /locus_tag="LRZ77_RS26460"
                     /old_locus_tag="LRZ77_26460"
     CDS             3995..4336
                     /locus_tag="LRZ77_RS26460"
                     /old_locus_tag="LRZ77_26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4545..>4973
                     /locus_tag="LRZ77_RS26465"
                     /old_locus_tag="LRZ77_26465"
                     /pseudo=""
     CDS             4545..>4973
                     /locus_tag="LRZ77_RS26465"
                     /old_locus_tag="LRZ77_26465"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5014..5511
                     /locus_tag="LRZ77_RS26470"
                     /old_locus_tag="LRZ77_26470"
     CDS             5014..5511
                     /locus_tag="LRZ77_RS26470"
                     /old_locus_tag="LRZ77_26470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            5518..5733
                     /locus_tag="LRZ77_RS26475"
                     /old_locus_tag="LRZ77_26475"
     CDS             5518..5733
                     /locus_tag="LRZ77_RS26475"
                     /old_locus_tag="LRZ77_26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            6194..6826
                     /locus_tag="LRZ77_RS26480"
                     /old_locus_tag="LRZ77_26480"
     CDS             6194..6826
                     /locus_tag="LRZ77_RS26480"
                     /old_locus_tag="LRZ77_26480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001567369.1"
                     /translation="MLRYELTPNNAGFILWGDSEALNELHELIHYIVDESPLIKVKDGF
                     MLSLAYDIRKAREGNRRVEQHQYDQHDTYKLYGVELLWPLVLVQSSILRNSMGYIQTDK
                     NQLSVMYAFEYLIESALTESERTTSNDIMLTVKYASDSDFNFIEDNIDSRCCYFISLSP
                     EQRKKQLISIVRSFHSLWGKYAREKQDIKMLNEMNNTSWVWPDNINW"
                     /name="hypothetical protein"
     Typing          3736..3911
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.449"
                     /coverage="81.0"
                     /evalue="5.74e-59"
     Typing          complement(3255..3753)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.04"
                     /coverage="92.0"
                     /evalue="5.84e-177"
     Typing          4327..5849
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gggactggtt tctgcacaga tagtctggat gttcagttca tgcatggctc gcccaaactg
       61 agtatgcccg tctccgcctg tggcgtgttt attgttaaca cgaaaaacac cggctttatc
      121 gctgtacagt gccagcggtt taccatgctt atcgatatag cgccgcgtgg cttcgaagta
      181 agaaaacgtg gactccgatt taacaaacaa cagttccatc agtttgctgg ttgcatcatc
      241 aacatagacc agcgcggtgc aggccgggcc acggccttca aaccagtcgt gatcacagcc
      301 atctatttgt atcagctcac cagtacacgg acgccggtac cgtggttgag ggatccttgc
      361 ggcacgttgt ttacggggaa cccataagcc agcccgcacc atgatgcgcc ggacagtttc
      421 tttgccaaga aacagtccgt ggagttcttc gagcttttca cgcgccagag tcggaccgaa
      481 atcagcataa cgcgtcttga tcagttccag agcctgatct gcgagcccgg gtggcaactg
      541 gcggttacca cgcatgccac atcgtctgct ggccatacca agcggtccgc cttcacggta
      601 acgggcaaga agtctgcggc attgcctgtc gctgataccg aggtgctcgg ccgcacggcg
      661 cgttgtgatg cgacgttcaa tgacgtcctg tataatcttg atccggttga tctctttcaa
      721 agtaaacact cctgagcttt ctgcgctcat gatatgcctc ccggtagttt aaacggacca
      781 gtgaagctta ccatagcgcg gacatctgaa ttgagccaca ggcggacatt actattgagc
      841 cattacaggc gcggtgcggg taagagatgt tatgttcaat cgaaatcaga aaaacgatcg
      901 gtaaagcgat cgtttttctg atagcatcat gttgatttta tataacgtgg gtgtaaagaa
      961 tgactagacc gattgctgtt gatttatttg ctggagcggg tgggctgagt ctcggtttcg
     1021 agcaagctgg ctttgatata atggcatcag ttgaaataga tccaatacat tgctctgctc
     1081 acaaatataa ctttcctatg tgcaaaacga tatgtagaag tgttgttgat gttaccggag
     1141 aatacgttag ggaagtttca ggtattggtg agaaagagat tgatgtagtg atcggaggcg
     1201 ctccctgcca aggcttttca ttaataggaa aacgcgcgct tgatgatgat agaaacaaac
     1261 tggtctatca ctatgtacgg ctggttcttg agctaaagcc taaatacttt gtttttgaaa
     1321 atgtaaaagg gttgacggtt ggtaagcata agcagttttt agtagagata atggatgcat
     1381 ttaaagatgg cgggtacgac gttgtaagcg attataaagt gctaaacgca gctgattatg
     1441 gagttcctca agatcgccgt cgtcttttct taatgggggg aaggaaagga attgctctgc
     1501 catcataccc tgagccatta tccattagaa caacggttgg ggacgctatt ggggatattc
     1561 ctgatgcgga aaaatatcct gagctatggg gtagagattg ggtttatgct aaatttggtg
     1621 agcaatcact atactcatcc tatttgcatg gcgtaggaaa agatcctagt gattttagct
     1681 acccaaggat ttttaataaa gacaaattaa cgtgttcttt actgacagat cactctgaca
     1741 tttctagagg gcggtttgtt gaaactttgc ctgacacggt agaaccaatc agcaggttta
     1801 aaaagctctc gatgaatggg ctatgtaata ctttaagggc aggaacagca agtgacagag
     1861 gtgcttttac atctcctcgt cctatacatc cgatctatcc tagggtcatc acggtcagag
     1921 aggcagcaag actgcattca tatcctgatt ggttcagatt tcatgtgact aagtggcatg
     1981 gatttagaca ggtcggtaat agtgttcctc ctatgctagc tagaagtgtt ggcttgcaga
     2041 ttatgaaggt tcttggtgtt acgccaaaaa aaccaacgaa gaaaatcaaa ctcggtgatg
     2101 aaagtttggt tgctatttct atgtccgatg ctgcatctat gtttggtgtg tcatcaaatg
     2161 taattgctaa gagaataaaa aatagtaaca aggaggttgt ttgtgagtaa ggataaaaaa
     2221 gaaaggtcca aaaatagata ccatcagttg gtagaaaaga tatttttcga taaatatgtt
     2281 gatggtgact tagaggtttt gtttgaaagg ttagatataa acagtgctgc aaaagattta
     2341 ggtatagaac tcccatctaa tgttggtgat gttatatatt caatcagatt tagaacgcct
     2401 atgccagaaa gtattctgtt gactcagcca gaaggtaagg agtggattat tgagctggct
     2461 ggtaggtcaa agtatcgatt tagattagta actattagta ggattttgcc aaatcctaat
     2521 atggtggcaa taaaaattcc agacaatacc ccggagataa ttacaggtta ttctttagac
     2581 gacgagcagg cattgctggc gaaggtgaga tataaccggc taatagatat atttctaggt
     2641 ataaccacat attctttaca gaatcattta agaactacgg tgaagggaat tggtcaaata
     2701 gagatcgatg aactttatgt aggtatagat aaatacggtt gccattatat aataccagtt
     2761 caagcaaaag gtgggacaga tcaaatatca atggtgcaaa catcgcaaga tacagcgtgg
     2821 tgtgaacaaa aatacccaaa tgttagatgt agagctattt ccgctcagtt tgtttcgagc
     2881 gatttgattg cactgtttga gctaaagata gacgaaggac aagtgaaatt gattgaggaa
     2941 aggcattaca aactggttcc ggtaaaagag ttagatagct ctgacatctc aaactaccgc
     3001 atgtctgtat aatttctgag aggatcttct tgagatcctt tcgttctagc cgtaatctcc
     3061 taagccgtaa acaaaaaaac caccgctacc aacggtggtt cgtttgccgg aatttagagc
     3121 taccaactct ttcttccgaa ggtaactggc tttcacggag cgcagatacc aaaactgtcc
     3181 tttcagtgta gccgtagtca ggccaccact tcaagaactc tgtagataac tctactccgc
     3241 tccgtgaatc cgattaccag tggctgctgc cagtggcgct tttgcatgtc ttttcgggtt
     3301 ggactcaaga cgatagttac cggacaaggc gcagcagtcg gactgaacgg ggggttcgtg
     3361 catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag
     3421 acaaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc
     3481 gcacgaggga gctaccaggg ggaaacgcct gggatcttta tagtcctgtc gggtttcgcc
     3541 accactgatt tgagcgtcag attttgtgat gcttgtcagg ggggcggagc ctatggaaaa
     3601 acggcaacgc cgcatccttt ttcctgactt tcctgacttt cctgactttc ctgacttgag
     3661 cgcgatctcg ttatctcgtc cttcgtgagc tggtaacgcc cgccgcagtc tcacggcagg
     3721 agcgcagcga ccgagtgagc gaggaggcgt catttcatcg ttacctgcat tttgcactta
     3781 cgccccgcag ccgtattttc ccccttgcca catgaagcac ttccccgaat ctccatctcg
     3841 ccgtaacata ctaagccagt atacactccg ctagcgctac gtgactggtt cagggctgcg
     3901 ccccgaaacc ccactgacgc gcctgcggct tgtccaaccc cgcgccgacc gggaaaggct
     3961 ttcccgtccg tcacggcgtt atcaggaggc gattctggca gataaacgca acaagatgct
     4021 cacgatgtgg gtgacggaag acgaacaccg gcggttgctt gagcggtgcg acggaaaaca
     4081 actggcggcg tggatgcgcc agacctgcct tgatgagaaa ccgacacgcg ccggcaggct
     4141 tccctcgatt tcgcccgcgc tgctgcgtca gcttgccggc atgggcaata acctgaacca
     4201 gatcgcccgg aaagtgaacg ccggtggtgc cggtcatgac cgggtgcaga tcgtggcagc
     4261 gctgatggcg atcgatgccg gtctggaacg gttgcggcat gccgtactgg aaaaagggcc
     4321 ggacgatgat tgttaaattc catccccggg gtcgcggcgg gggcgccggt cctgtggatt
     4381 atcttctggg taaagaccgg cagcgtgaag gtgccagcgt cctgcagggg aagccggaag
     4441 aagtccggga gctgattgat gcgtcgccgt atgtgaaaaa atatacctcc ggggtgctgt
     4501 cattcgcgga ggcggacctg ccgccgggtc agcgtgaaaa actgatggcc agctttgagc
     4561 gggttctgat gcccggactc gataaagacc agtacagcat tctgtgggtg gaacacgcag
     4621 acaagggtcg tcttgagctg aattttctga tccccaatac cgaattactg acggggaaac
     4681 gcctccagcc gtattacgat cgtgccgacc ggccgcgcat cgatgcgtgg cagacagtgg
     4741 tcaacggcag gctggggcta catgatccga acgacccgga gaaccggcgg gcgctggtca
     4801 cgccttcagg actgccagaa acgaagcagg aagccgcaga gacgattaca cggggtttgc
     4861 tgatgttagc ctcatcagga gagctgaaaa cgcgtgatga cgtcacaggg gcgctcacag
     4921 cggccggttt tgaggtggtc agaaccacaa aaaacagtat cagcattgcc gacccggacg
     4981 gggggcgaaa tatccgactg aggggagcca tttatgagca gtcttttaac ggcctcgagg
     5041 gacttcgagg agaaatcgaa agcacagcag cagcttaccg aagagaggct gaaagccgca
     5101 ttcagcaagc acgagaacgt tgtcaaatcg gaactgaacg caagcgcgaa gagaatcaac
     5161 gacgccatca gcgcccacga acaggggatg aacgcggcga tgcagtcgaa ccggctgagc
     5221 gtgctgcgaa tggtcgggcg gacgtggctg accatcacca tggtgtcagg gctgctgttc
     5281 gccagcctga gcggggtgct gtggtaccag gggaaccgga tagcggcgaa cctggcggaa
     5341 atcaggcagc agagagatac gctgtcgaaa ctgcacatgc agacgtgggg cgtgacctat
     5401 ctggagaacc gcaacggacg gtttctggtg ctgccggagg ggatgaaagc cgaaacgggc
     5461 cggacggtgg acaacaagac gcggaacgcg gtgaagctgg tacgggagta gagcagaatg
     5521 acggaactgg aaaaacaatt gctgagcgcg ttagagcagc tacagcagga ctactcgaga
     5581 aggctggacg agtgggagag cgccttcgag gaatggcgga agatgtctgg tcttatgcaa
     5641 cgggagaacg cggcgctgag cgagcgcgtg tcgggcttga gcaggcaggt gcagagtttg
     5701 agcgagcagc tgcgccggtt atcgacaggc tgaatgagat tgagcaacag cgatacgggc
     5761 aaacaacgga aaaggaacca gagaagacag cagatctggt accggaacgg caacgcacat
     5821 tatctcatgg tgacagggag tatggagact gataaacgcc cgtctcaggt ggcctgagcg
     5881 tgacggggat catggccatt ttctgcgatt tcagcacaca gagagtcttt tttaaccgaa
     5941 atcaccggct atgcagtgat gtgaataagt gatgcatatg cgtaaaataa atcaggtgcg
     6001 tttctggtgg ttttcggtgt acttgccacc accttttgcc cgtcagccat cagaaacgcc
     6061 ctgagtgcct ccggcgctgt caaggctcaa ttgaaatgtc cgttatccag ctcaattaaa
     6121 atgaccactt tgatctctca ttctctttac tgatagactt tcctccgact gaaacaacag
     6181 gatgattgag cccatgcttc gatacgagtt aacgccgaac aatgcaggtt ttatactgtg
     6241 gggagattca gaagccctga atgaattaca tgaactcatt cattacatcg tggatgaaag
     6301 cccactgatt aaagttaaag acggatttat gttatccctt gcctatgata ttcgtaaagc
     6361 acgggaaggt aatcgtcgtg ttgagcaaca tcagtatgat caacatgata catataagct
     6421 ttatggtgtt gagcttttat ggcctctggt cctggtacag tcctcaatac tcagaaactc
     6481 aatgggttat attcagacag acaaaaacca gctgtctgtc atgtatgcct ttgaatacct
     6541 gatagaatca gcattaacag agtctgagag aacaacgtcg aatgatatta tgctaacagt
     6601 aaaatatgca tcagactctg attttaattt cattgaggat aatattgaca gcaggtgctg
     6661 ctattttatc agcctatctc cggagcaaag aaaaaagcag ttaatcagta ttgttcgttc
     6721 ttttcattca ttatggggta agtatgcccg tgaaaagcag gacataaaga tgctgaacga
     6781 aatgaataat acatcatggg tctggccgga caatatcaac tggtgagcaa ccactgtccg
     6841 gccagtgagt accatcagcg ggctctttta ccaaaaatgt cctctgaacg tgactgaagc
     6901 tgtttgagtg cttcgagcat gtcatcatta tccagtgagc gctgggattt cttcccttcc
     6961 tgctttggcc gtcgtcggga agggccatct ccagcgggaa ttgactgaga gcgcgtgtta
     7021 tcccgcttgc tctgcaccag actgataaac tccagggttc ggccaagacg cttgttatcg
     7081 acaatcgcgc cctggtcgat ttctgacagt cggtcgtagg tagagtaggg aagtagcgta
     7141 ccgttcaggc gcagctcttt tctgccatca ggatagtgat acacatcgat atatttacct
     7201 attgcacggc gactcagttc gctgtcttca atcaggtaca acattttatc atattgtatc
     7261 gtcaacgatt ttgagacttt acgtttttca cgaacagtga aaataagccc caggtcctca
     7321 tcatgttcta cagcacggtg tacgtcaaaa tcatgtcgcg gtactttgcc aaaacggcgg
     7381 ttatagtcag ccatataggc ctcagcgaag tcatttgcag cctccattga acaaatgccc
     7441 tgtaaccgca gctctttgac cagacgatcc tgtaaagtga ggtgagctcg ttctacacgc
     7501 cctttggc
//
