LOCUS       NZ_CP089003             4160 bp    DNA     circular CON 12-FEB-2024
DEFINITION  Klebsiella pneumoniae strain 56661 plasmid p56661-4.160, complete
            sequence.
ACCESSION   NZ_CP089003
VERSION     NZ_CP089003.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23552368
            Assembly: GCF_021166135.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Chen,L., Hao,M., Lin,Y. and Kreiswirth,B.
  TITLE     Whole genome sequencing of K. pneumoniae isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Chen,L., Hao,M., Lin,Y. and Kreiswirth,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 111 Ideation Way, Nutley, NJ 07110,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-SEP-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/12/2024 03:45:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,968
            CDSs (total)                      :: 5,844
            Genes (coding)                    :: 5,674
            CDSs (with protein)               :: 5,674
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 70 of 170
            Pseudo Genes (incomplete)         :: 104 of 170
            Pseudo Genes (internal stop)      :: 30 of 170
            Pseudo Genes (multiple problems)  :: 31 of 170
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP089003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="56661"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p56661-4.160"
                     /geo_loc_name="Russia"
     gene            346..831
                     /locus_tag="LRZ77_RS26350"
                     /old_locus_tag="LRZ77_26350"
     CDS             346..831
                     /locus_tag="LRZ77_RS26350"
                     /old_locus_tag="LRZ77_26350"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_048332377.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEDGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            835..1047
                     /locus_tag="LRZ77_RS26355"
                     /old_locus_tag="LRZ77_26355"
     CDS             835..1047
                     /locus_tag="LRZ77_RS26355"
                     /old_locus_tag="LRZ77_26355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1733..2650
                     /locus_tag="LRZ77_RS26360"
                     /old_locus_tag="LRZ77_26360"
     CDS             1733..2650
                     /locus_tag="LRZ77_RS26360"
                     /old_locus_tag="LRZ77_26360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_116955814.1"
                     /translation="MSNAALHLFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTITNPDNGHAHLLYALKTSIRTAPDGKIK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHAHWKIAVWQPELYTLDWLADFLELNAA
                     NDKEIIADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLKACYERASAYNLQFSAPLD
                     DKEVMGIAKSIAKWTGSNFSKEAFDEYVIRTHTSDIQRIRGRKGGAIGGKKSNSGGRPS
                     LGEPWIELGVSRRTYFRNKKRKGC"
                     /name="replication initiation protein"
     gene            3508..3828
                     /locus_tag="LRZ77_RS26365"
                     /old_locus_tag="LRZ77_26365"
     CDS             3508..3828
                     /locus_tag="LRZ77_RS26365"
                     /old_locus_tag="LRZ77_26365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024243919.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLETGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          4..1163
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.416"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3819..4159
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.614"
                     /coverage="87.0"
                     /evalue="1.1e-66"
ORIGIN
        1 tgaccgggaa acgcctgcag ccgtactacg atcgcgccga ccggccgcgc atcgatgcct
       61 ggcagaccgt ggtgaacggc agactggggc tgcatgatcc aaacgccccg gagaaccggc
      121 ggttactggt cacgccgtcc gcactgccgg aaacgaagat ggaagccgct caggcgatta
      181 caagggggtt gctggcctta gcctcatccg gggagctaaa aacgcgcgag gacgtcacag
      241 gggcgctcac agcggcaggt tttgaggtgg tccgcaccac aaaaaacagt atcagcattg
      301 ccgacccgga cggggggcga aacatccgac tgaagggagc catttatgag cagtctttta
      361 gcgctggcga aggacttgga gcagcaatcg aaagcgcagc agcagagtac cggcgagatg
      421 ctgaaagccg cattcagcga gcacgagaag tctgtaaaag cggaactgag cgcaagcgcg
      481 aagagaatca gcgacgccat cagcgcccac gagcagggta tgaccgcggc gatgcagtcg
      541 aaccgcctga gcgtgctgcg tatggtcggc cggacgtggc tgaccatcac cctggtctcg
      601 gtactgctga tcgccacgag cgggagcatt ctgtggtggc aggggcagca gatcaccggg
      661 aattaccaga ctatccgggc gcaggagcgg acgcaggcca tgctgtcgga gaagaaccac
      721 ggcgtgcagc tgtcgctctg cggggagaag aaactgtcct gcgtcaaagt gaacccgaaa
      781 gcgggagctt acggggagga cgggaactgg atggtactgg agaggaaata gcccatgacg
      841 gaactggaaa aacagttgct gaccgcatta gagcagctac atcaggacta ctcgcaaagg
      901 ctggacgagt gggagagcgc cttcgcggaa tggcagaaga tgtctgggct tatgcaacgg
      961 gagaacgcgg cactgaacga gcgcgtgacg cgcttgagtc agcaggtggc gaacttgagc
     1021 aggcagttac agcgtttgag ccagtagtgc agcggcatga aatggcggtg gccgccgaac
     1081 gggcatacga gcagcgacag cacgaaaaag cgctggcgga agcccgttcc cgtcagcgga
     1141 gctataacgg tccgacgctg ggctgacaga gcttcaccag tgacaacccc cacgaagaag
     1201 ggctcataaa tttcccgcta aatacggtcg tgatgaaaac atcacggcgt atacaacgga
     1261 gggattatga gactttttaa cgtgattgag acggtgctga actgcctgta catggcgctg
     1321 gagatctggg actggctgga gaagcatttt cctgttttta aggcgttcat ccggattctg
     1381 gcctgaaatc agaccgtatg catctggctg catgaccatg caacagtgta atatccagcg
     1441 gctgcgtttc tggtgttttt tcgtcatgtc tgtcgccact ttctcgcgtc tggcaccgga
     1501 aacgcagcca accccgtttc agcggtgcgg gcaacggatg ttatggtaaa ttgttgtggg
     1561 ttcgacagaa gttgccagac ggtgtaacgt taagacaaga aaaaacgaag ccccggaaat
     1621 catgctcaat cttggcggat gcacatgata accagggcca ggtacgaaac cttgaaggaa
     1681 tcttagcaca tgaaccgtgc cagacaacag cccgccataa gcgcaggccg gattgagtaa
     1741 cgctgcactc catcttttca atgagcgttt gccgcataag ccgtactttt cggacgattt
     1801 gcactttggt gtacgaattg ccggtaaaga acgcgccatt ctggcgaaat acatccagtt
     1861 caatcagcct cacgccatgt tctggctggg attcgacgtc gatcgcgccg gtgctgccat
     1921 tgattggagt gatcgtaatg ctccagcgcc tacgctgacc atcactaatc ctgacaacgg
     1981 acatgcgcac ctgctgtacg cactaaaaac ctcaatccgc actgcgccgg atggtaaaat
     2041 taaaccgctg cgttatgccg ccgccgtgga gaacgcgcta cgtaaaaaat tagaggccga
     2101 cgcgggctat tcgggactaa tctgcaaaaa tccgaaccat gcccactgga aaattgctgt
     2161 gtggcagccg gaactctaca cgctggactg gcttgctgat tttctcgaac taaacgccgc
     2221 gaatgacaaa gaaatcattg ctgactacgg cttaggccgt aactgcacct tgttcgacaa
     2281 gacccggaag tgggcatacc gcgccatccg tcagggttgg cctgagtatg aacagtggct
     2341 gaaagcctgc tacgaacgcg ccagtgctta taaccttcaa ttttcagcgc ctcttgacga
     2401 taaagaagta atggggattg ctaaaagtat tgctaaatgg actggctcta attttagtaa
     2461 agaagctttt gatgaatatg ttatacgtac tcatacctct gatatacaga gaataagagg
     2521 tagaaaaggg ggggctatag gtggaaaaaa aagtaattca ggtggaagac ctagtttagg
     2581 tgagccatgg attgaacttg gtgtgagtcg acgaacttac tttaggaata aaaaaaggaa
     2641 ggggtgttaa tgcggttaat aattttaatt tcatctttgt tactgatcac ttcatgcggc
     2701 gataagaata agtcagaatt tatggatggt tgcactgggg ggagtacgag tgattacgct
     2761 cataaagtct gttcttgtgc atatgataaa atgaaagata catatggtga cccggaaaag
     2821 tgggaatcga ggatttatag agagggggca gataacttga tttcagcaat gaaagtgtca
     2881 attgaaagtt gccgatagga aaaaggctgc taatgcagcc tttttcctat ttctttaatg
     2941 aaacataagc tataaaaata agaagtagta tcccaccaac aatcgctcct agcgttggga
     3001 accatatgaa tggccaaact aaagctctac ctatgttata ggcgacgcct ttataattaa
     3061 aatcacccca tatacttccg tacacggcag ttccaacagc taatagtatg tatatactaa
     3121 aaattgtgct ttttatgttc atttaacaat tccattttta ccattgcgta ctgtgcatct
     3181 tgattctaac agaactgaat catcgaccac cctcccattt ccttcacttc aaccgcgtgg
     3241 cactaaacaa agcctatatc agataacagc cctctctagc agggccgctt ttttgcagtc
     3301 agtccgcatt gaatctgaca tactgcacta taccttaagc cagtatacac tccgctagcg
     3361 ctacgtgact ggttcagggc tgcgccccga aacccgctaa aagcgactga cgcgcctgcg
     3421 gcttgtccaa caccgtgccg cccgggaaaa atttcccgcc cgtcccggtg ttatcaggag
     3481 gctactgtgg cagataaacg caacaagatg ctcacgatgt gggtgacgga agacgaacac
     3541 cggcggctgc tcgagcggtg cgacggcagg cagctggcgg cgtggatgcg tcagacctgc
     3601 ctcgatgaga agccggcacg tgccggcaga ctgccttcga tatcgcctgc gctgctgcgc
     3661 cagcttgccg gtatgggtaa caacctgaac cagatcgccc ggaaagtgaa tgccggtggt
     3721 gccggccatg atcgggtgca gatcgtggcc gccctgatgg caatcgacgc gggactggag
     3781 cggctgcggc atgcggtgct ggaaacaggg ggggatgatg atcgttaaat tccatccccg
     3841 ggggcgtggt ggtggtgccg gtccggtgga ttacctgctg ggaaaagacc ggcagcgcga
     3901 gggggcgacc gtgctgcagg gaaagccgga ggaagtccgg gagcttatcg atgcctcgcc
     3961 gtatgcgaaa aagtacacct ccggtgtgct gtcgttcgcg gaggctgaac tgccgcccgg
     4021 gcagcgggag cagatcatgg ccagctttga gcgggttctg atgcccgggc tcgataaaga
     4081 ccagtacagc attctgtggg tggaacactc cgacaagggg cggctggagc tgaatttcct
     4141 gatccccaac actgaactgc
//
