LOCUS       NZ_CP088993            11972 bp    DNA     circular CON 12-FEB-2024
DEFINITION  Klebsiella pneumoniae strain 50700 plasmid p50700-12.0, complete
            sequence.
ACCESSION   NZ_CP088993
VERSION     NZ_CP088993.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23552370
            Assembly: GCF_021166115.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11972)
  AUTHORS   Chen,L., Hao,M., Lin,Y. and Kreiswirth,B.
  TITLE     Whole genome sequencing of K. pneumoniae isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11972)
  AUTHORS   Chen,L., Hao,M., Lin,Y. and Kreiswirth,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 111 Ideation Way, Nutley, NJ 07110,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-SEP-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/12/2024 03:19:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,968
            CDSs (total)                      :: 5,849
            Genes (coding)                    :: 5,656
            CDSs (with protein)               :: 5,656
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 193
            CDSs (without protein)            :: 193
            Pseudo Genes (ambiguous residues) :: 0 of 193
            Pseudo Genes (frameshifted)       :: 89 of 193
            Pseudo Genes (incomplete)         :: 115 of 193
            Pseudo Genes (internal stop)      :: 22 of 193
            Pseudo Genes (multiple problems)  :: 33 of 193
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP088993.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11972
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="50700"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p50700-12.0"
     gene            join(11032..11972,1..547)
                     /gene="ltrA"
                     /locus_tag="LRZ81_RS27085"
                     /old_locus_tag="LRZ81_27085"
     CDS             join(11032..11972,1..547)
                     /gene="ltrA"
                     /locus_tag="LRZ81_RS27085"
                     /old_locus_tag="LRZ81_27085"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            652..2337
                     /locus_tag="LRZ81_RS27090"
                     /old_locus_tag="LRZ81_27090"
     CDS             652..2337
                     /locus_tag="LRZ81_RS27090"
                     /old_locus_tag="LRZ81_27090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            2347..2604
                     /locus_tag="LRZ81_RS27095"
                     /old_locus_tag="LRZ81_27095"
     CDS             2347..2604
                     /locus_tag="LRZ81_RS27095"
                     /old_locus_tag="LRZ81_27095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            2689..2838
                     /locus_tag="LRZ81_RS27100"
                     /old_locus_tag="LRZ81_27100"
     CDS             2689..2838
                     /locus_tag="LRZ81_RS27100"
                     /old_locus_tag="LRZ81_27100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            4324..6288
                     /locus_tag="LRZ81_RS27105"
                     /old_locus_tag="LRZ81_27105"
     CDS             4324..6288
                     /locus_tag="LRZ81_RS27105"
                     /old_locus_tag="LRZ81_27105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            6288..7019
                     /gene="mobC"
                     /locus_tag="LRZ81_RS27110"
                     /old_locus_tag="LRZ81_27110"
     CDS             6288..7019
                     /gene="mobC"
                     /locus_tag="LRZ81_RS27110"
                     /old_locus_tag="LRZ81_27110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            7026..7556
                     /locus_tag="LRZ81_RS27115"
                     /old_locus_tag="LRZ81_27115"
     CDS             7026..7556
                     /locus_tag="LRZ81_RS27115"
                     /old_locus_tag="LRZ81_27115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7583..7762)
                     /locus_tag="LRZ81_RS27120"
                     /old_locus_tag="LRZ81_27120"
     CDS             complement(7583..7762)
                     /locus_tag="LRZ81_RS27120"
                     /old_locus_tag="LRZ81_27120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7788..8216)
                     /locus_tag="LRZ81_RS27125"
                     /old_locus_tag="LRZ81_27125"
     CDS             complement(7788..8216)
                     /locus_tag="LRZ81_RS27125"
                     /old_locus_tag="LRZ81_27125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(8380..8694)
                     /locus_tag="LRZ81_RS27130"
                     /old_locus_tag="LRZ81_27130"
     CDS             complement(8380..8694)
                     /locus_tag="LRZ81_RS27130"
                     /old_locus_tag="LRZ81_27130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8682..8960)
                     /locus_tag="LRZ81_RS27135"
                     /old_locus_tag="LRZ81_27135"
     CDS             complement(8682..8960)
                     /locus_tag="LRZ81_RS27135"
                     /old_locus_tag="LRZ81_27135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(9135..9500)
                     /locus_tag="LRZ81_RS27140"
                     /old_locus_tag="LRZ81_27140"
     CDS             complement(9135..9500)
                     /locus_tag="LRZ81_RS27140"
                     /old_locus_tag="LRZ81_27140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(9497..9868)
                     /gene="tolA"
                     /locus_tag="LRZ81_RS27145"
                     /old_locus_tag="LRZ81_27145"
     CDS             complement(9497..9868)
                     /gene="tolA"
                     /locus_tag="LRZ81_RS27145"
                     /old_locus_tag="LRZ81_27145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(10142..10387)
                     /locus_tag="LRZ81_RS27150"
                     /old_locus_tag="LRZ81_27150"
     CDS             complement(10142..10387)
                     /locus_tag="LRZ81_RS27150"
                     /old_locus_tag="LRZ81_27150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             1..7337
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2942..3522)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.846"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6289..7016
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 cttccgtata gccatgcagc gcagactgag ggagttcgga ctcacggttc acccggagaa
       61 aacccgtctg atggagttcg gccgcttcgc tgccgaaaac cgtgccatca ggggaaaagg
      121 caaaccagaa acgttcaact tcctcgggtt cacgcacatc agcgggaaag atcgcaacgg
      181 caggttcatg ctgatacgaa agacccgccg ggatcggatg acggcaactc tgaaagccat
      241 caaagacggt ctgcgaaggc gctggcatta ctcaatcccc gaacagggaa aatggctcag
      301 gagagtggtt cagggatacc tgaactatca ctcggtaccg ggcaacttcc ccaccatgca
      361 gaagttcagg acacacgtaa caaacctctg gcgccgggcg ctcaggcgca ggagccagaa
      421 ggatgatacg acctggacga aagcaaacaa actggcagcc gcatggctac caagggttcg
      481 ggttcttcat ccatggcctg tggagcggtt caccgccaga cacccgaggc aggagcccgg
      541 tgcgtaaatc gcgcacgccg ggatctgtgc ggggggtatc cggtaacggg tatccctacc
      601 gcgacatgtt ggctttaatt tattgtttta aatgtcaaag aggaaaacgg tatgagtggt
      661 ggagacggtc gaggtccggg taattcaggt ctgggacata atggtggtca ggccagtggg
      721 aatgtgaacg gtacgtctgg taaaggtggc ccttcatcag gtggtggtac ggatccaaac
      781 agcgggccgg gctggggtac gacgcatacg ccgaatggag atattcataa ctataatccg
      841 ggggagtttg gtaacggcgg gagtaaaccc ggtggtaatg gtggtaacag cggcaatcat
      901 agcggtagct ctggtggcgg acagtcttcg gccactgcga tggccttcgg tctgcctgcc
      961 ctggctactc cgggggctga aggactggct ttatcagttt ccggcgatgc gttgtcggcc
     1021 gccgttgctg atgtgctggc tgccctgaag gggccgttta agtttggtct gtgggggatt
     1081 gcgatctacg gtgtgctgcc ttctgagatt gcaaaagatg atccgaaaat gatgtcaaaa
     1141 attatgacgt cattaccggc cgatgcggtg acggagactc cggcaagtac tttaccactg
     1201 gaccaggcga cggttcgtgt cagacaacgg gttgtggatg tggtgaagga tgagcggcag
     1261 catattgcgg ttgtcgcagg tcggccaatg agtgtccctg tggtggatgc gaaaccgaca
     1321 aaacgtccgg gggtattcag tgtgtcgatt ccgggtctcc cgtctctgca ggtgagcgta
     1381 ccgaaaggtg ttccgacagc gaaagccccg ccaaaaggca ttgttgctga aaaaggtgat
     1441 tcacgtccgg ctggttttac agccggtggt aactcccgtg aggccgttat tcgtttcccg
     1501 aaagagaccg gacagaagcc ggtttatgtg tcggtgacag atgttcttac cccggcacag
     1561 gtaaaacagc gtcaggagga agaaaagcgt cgccagcagg catgggacgc cgctcatccg
     1621 gaagaggggc tgaaaagaga ctatgataaa gcgaaagctg agctggatgc cgaagataaa
     1681 aatattgcga ccttaaacag ccgcattgca tcgacagaga aggcgctccc cggtgcaagg
     1741 gctgctgttc aggaagccga taaaaaggta aaagaggcag aggcaaataa agatgatttt
     1801 gtgacttata acccgcctca tgaatatggc tccgggtggc aggatcaggt tcgctatctt
     1861 gataaggata ttcagaatca gaatgagaaa ttaaaagcgg ctcaggcatc tttaaacgca
     1921 atgaatgaat ccttatccag agataaggct gcacttaccg gggcgatgga gagccggaaa
     1981 caaaaggaga aaaaagcgaa ggatgcagaa aataagttaa atgaggaaaa gaacaagcct
     2041 cgcaagggga ctaaagacta cggccatgat tatcatccag tacctaaaac agaagacata
     2101 aagggacttg gagagttaaa gcgaggtgat cctaaaacac ctaaacaagg tggtggtggt
     2161 aagcgggccc gatggtatgg cgataaaaaa cgtaaaattt atgaatggga ttcccagcac
     2221 ggtgagcttg aagggtatcg tgccagtgat ggcgaacacc taggcgcatt tgatcccaaa
     2281 acgggtaagc aggttaaagg gccagatcca aaacggaaca ttaaaaaata tctttgagag
     2341 gtaaatatgg ggcttaaatt acgtttgaca tggtttgata agaaaactga agagtttaaa
     2401 ggggaagagt attctaaaga ctttggtgac gatggttctg tcattgaaag cttgggaatg
     2461 cctttaaagg ataatattaa taatggtgag tttgatttga agaaagaatg ggttccttta
     2521 ctacagccat tttttaaaaa tcaaataaaa cctgatgtgg ataattgtta tatagctttt
     2581 gattatcggg acggtaactg gtaacaaaaa attatcaagg atgatttgaa atactgactt
     2641 ccggtgttca gggatgaatg ccggagtctt taaatacagg gaaagtatat gaaaaaggta
     2701 aagactattt ttctttttat attgattgtt gctggctttc ttctggccgc atgtcaggca
     2761 aattatatcc gggatgttca gggcggaacg gtggcaccat cgtcctcctc tgaactgacg
     2821 gggatcgcgg ttcagtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2881 taatctcttg cactgtaaac gaaaaaaccg cctggggagg cggtttgatc gaaggttaag
     2941 tcagttgggg aactgcttaa ccgggtaact gggtatacga gaccgcagat accaaaactg
     3001 ttctttcagt gtagccggac tttggcgcac acttcaagag caaccgcgtg tttagctaaa
     3061 caaatcctct gcgaactccc agttaccaat ggctgctgcc agtggcgttt ttgcgtgtcc
     3121 gtccgggttg gactcaagat gatagttacc ggaaagggcg cagcagtcgg gctgaacggg
     3181 gggttcgtgc atacagccca gcttggagcg aacgacctaa accgagccga gataccagca
     3241 gcgtgagcta tgagaaagcg ccacacttcc cgcaagggag aacggcggaa caggtatccg
     3301 gtaaacggca gggtcggaac aggagagcgc aagagggagc gacctaccgg aaacggtggg
     3361 gatctttaag tcctgtcggg tttcgcccgt actgtcagat tcatggttga gcctcacggc
     3421 tcccacagat gcaccgaaaa agcgtctgtt tatgtgaact ctggcaggag ggcggagcct
     3481 atggaaaaac gccaccggcg cggccctgcc gttttgcctc acatgttagt cccctgctta
     3541 tccacggaat ctgtgggtaa ctttgtatgt gtccgcagcg cccgccgcag tctcacgccc
     3601 ggagcgtagc gaccgagtga gcgaggaggc gcaatttccc cggcacctgc aagacggtca
     3661 ccggacgcgc tgatgtggga tattgtcccc tgattaagtc tttctattcc ctgacgatca
     3721 attatcagga taggtgaacc atgtcggttt ttcgctgaac attttacgtt aacctatctc
     3781 tgttgctttt ttctccttgc ggagaaatgc aagtcatccc cgctggggcg tggctgactt
     3841 caggggaaaa gaccttgctt acgctggctg gacgccaccg acgcacggtc ttttcccctc
     3901 tttctggctc tgtccggctg gacgccggcc acgccagaaa ttcacccggc ccccgtcagg
     3961 ctcacgcctg accacccaca aaaatccacg gtgctggacg cacctgacga agcagcaaag
     4021 ctgctcctga ttttgcgggt ggtcagccgc tcgctatggc gaactctgac gggggccgga
     4081 acaccggaaa agaccgcact ggggtgccgc cttttcctct cacacgctgg ggcgtgctcg
     4141 ttgttcctcc ggggctgcgc cttttccgca tggggcggaa agaggctggc ctccgcggtg
     4201 aaagctgaac gcaatcacca cggacgtgac cgccgccgcc atgccttggg gcatgtccgc
     4261 ggcttgtgca gcgggggctg cgcatcacgc cggtaaaccc gtcgtctgaa ggagagagat
     4321 tgcatgttta atacggattc gcttgcctgg cagtggcata tcccggagct ggcccggcag
     4381 atttacccgt gggtcagctg gctgtaccgg gagtattaca gtctggccgg tttttttctg
     4441 gttatcggcc tgtttttcgg tctggctgaa ctctgggccg gccgaatgcg ttttgtgggg
     4501 ctgagccata aggcgcggcg ggagtatgcc gatcgctata agcgcgataa taaccggact
     4561 gtgccatttt cggtgctggc ctcgctggat tcacccgggc ggatggtcag ggcacggcga
     4621 catgtctgga actacgcgta ctggctgacc ggcgctgccg gtctggccct tctggggggg
     4681 ctggttgtcg ccgtgtttat cgagagctgg ggagaaaccg tcctgcgccc ggacctctat
     4741 gcgaacgcag attattacct gctgcagccg gctaacctgt tcgggatagc ctggtgtgcc
     4801 ggagggcttg tcgccggcgt ccttctggcg cggctgctgg cgttcctgct gcttgatggt
     4861 cattttgcgg ccgctgacga ggcggtgaac gcgaaactga atcaggagag ccggcgcagc
     4921 agccagcgga ccggagaaat gacagacgtc cgccacctgc atttcgggga gccggtaccg
     4981 gttaacgcgc tggccgattt ttccacggag caggcccgga aacagcaggc ggtgtttctg
     5041 ggtaaggatg agcagggcca gccggtgctg gtccctcgtg atacctggcg caagaccaat
     5101 attcagatcc tcggcctgcc cgggagcggc aagagcgcga tggccaccaa cgcgctcatt
     5161 cgctgcgtac gcgatttcgg ggacgctgtg gtgtacttcg accccaaagg ggacgcgtgg
     5221 gcgccgcacg tcttccgggc gcactgcccg gacttcaccc tgctggatct gcggccgggg
     5281 aaaccggcgc agctgaatct gttccgcgat ctcgaccagt acgccctgaa gaatctgctg
     5341 gttgccgggt ttaacctgag tgagaccggc gacgtggcgg accactaccg catcagcgag
     5401 cagaaagcgg cgaagctgat tgcggagcag ttcccgcagg gggcaaacat tcagcaggtg
     5461 ctggcggcgg cgtacgcgtt gccggaggcc ctgaaaaagg acgtgaaggg gctgatcacc
     5521 aagctggaga acgtggccga cctgagcgtc ctgcagacgg acagcggtat cgatgtggcg
     5581 gggataatca acggcggcgg ctgcctgtac gtcatcggct cgatggacga tgaggcggtg
     5641 atccgggtgc agaagatgct gtttgcccgc tgcgcgcaaa tcatcattgc gcgggatgag
     5701 ttccggaggt ggccgcacgc gagcatcatg ctcgatgaga ttaaatatct gctgtcgaag
     5761 tacgtgctga acgcgctggg gacgctgcgc agccgggact gcaacctgct gctcgcgcac
     5821 cagtcgctgg gcgacttcgg tcagtgcggg caggatctgc ccgctgattt tgtgaaaacc
     5881 acggtactgg ataacacgcc gatccgctgg ttctatcgcg cggccagcca ggagtccgcg
     5941 cagtgggcgg cgggccagac cggggagatc cgggtggacg tggagcgccg ccgggccagc
     6001 cgggaggcgg ggaacgtgga gcacatcagc ggcgacagct tcatccagaa ggaggcgcgg
     6061 ccgctgtttg acgtgaacac cctgcaacac ctgcctgacg ggttcgcggt gatgaccggg
     6121 ctgggcgtgg cgcggctggc gttcagcagc ccgctgcggg ttgagcgccg ggaaatttcg
     6181 ctgaaaacct tcccggtgct ggcgaaaacg gacccgctgg cggagtacct gcctccggcc
     6241 gacggcggcg gaccaaaagc ggatgatgat tttgcggggc tttactgatg acactggagc
     6301 agtacaacgt cagccacgcg aagcggcagg cgcggaacgc ggagaaaaca cggctgaccc
     6361 tgcgctggct gcgggaagag ctgtgttcca cggcggagct ggttgcgcga cggctgggga
     6421 ttgcggcggt acagccggtt taccgcttcc tggacagtct ggtggcgaaa gggctgctgg
     6481 tccgggcgaa atacccggtt gacggccgtc aggtcagcgt gtgggggctg acgccgcacg
     6541 gggtggcgtt ctcgttcgac gaggacgagc cgctgacgga cgttattccg tttcagccgt
     6601 caagggtgtc ggcggcgcag ctgccgcacc ggctggccgt gcagtccctg cgcctctcca
     6661 tggagggccg gggggccacc ggctggcggt acctgcaccg tatggccctg aaagggatga
     6721 aagtgccgga cgcgctggcg gagctggacg gcagaacggt ggcatttgag gtagagcgca
     6781 ccgtcaagag ccgcaggcgg tatcaggaag tggtgtccgg gtatctgttc aaccgccggg
     6841 cgaacggcat cgatgaaatc tggtacatct gccctgaccg gccgacgctt gtccgggtac
     6901 agcgggcgat tctggcggtg gatgaaatcg tgaatccaca gaccggcgag gcccgcaaaa
     6961 cggtggagct ggaccgggag aggctgttcg cgtgctttaa atttatgaca acggagtgag
     7021 ggattatggc gctgtacatg gtatttattg aggacgtgga agacaaagac ggcgagcgct
     7081 gcattatgcc aaccagcgag catctggaag acatggtggt gctcgcctgg cagaccgggg
     7141 cgtatgtttt ctgttttgtc gaccgggccc agcatctggc gatgatgaaa tccatcggga
     7201 tgtatgacga gcggctcaac agcgacgaaa aagccacgta ctttttcacc ggtgaaaatc
     7261 cgcaatggga acgggacaaa cccacaggaa tgaagctgtg tgattacctc tatcgaaagg
     7321 tggagcggtt cgccatcgag aacggtgtgc aggtggttga tgccgggaag tgctcaccgg
     7381 agcagattaa cgcgatgatt gaggtggtca gcgagcggca cggggaaagc gcggtgaacg
     7441 agaagctgaa agcggagctg atgcgggaat ataacagccg ctattttaac gtgccggtgc
     7501 ctgaactgcc ggaggacaaa cccgcccgcc ggtggtggcc gttcggcaaa aaataacgcc
     7561 agcaccggca tggccggtgc tgttattctt ccccgagctt atcccgcatc acctgccaga
     7621 gtgtttccgc atcctcgtga agccgttcgc acatgtcggc cggggtatcc atgtccagct
     7681 cgttcagctt ttccagcaac agcagcgact gatccttaat gaatttagcc atattgaggg
     7741 cagttttttc ctgcttcgtc atgttctcca ttcccttcgg taagttctca gctaaaccag
     7801 ccggaccaga agcgtttttt cttctgtgtc agttctgttg tcaatctgtc cacttcggcc
     7861 tggagtttcc ggcgctcatc atcgtgctgg cggcgttctt cttcccggga tgttttatct
     7921 tccagcatca gggtgacagc ctgccggaga tcggccagtt ccttttgcat tgccagaagc
     7981 tgttcctgtg ggaaattttt ttgtgtattt tcacggtgtg aaatgccggg aatttgtttc
     8041 tcagggatat tcacatttcc gtatacacgg atcagttctg acacatccac gacagggttg
     8101 tttttcccgt cacgtgacac ggtaaccttt ccctgtttta catgattgta tagcgttctt
     8161 ctggtaatgc ccgcggcttg tgctgcctgt gaaaggttga gcaatgtttt cgccactgta
     8221 tacccctgat tttatcgggt gtgaaaggtg tgtaatttct cacggtaaat gtatcgctga
     8281 attttacccg tttttccgtt caggatgtgg ttttctgacg cgctgcgctt gtcaaaccgg
     8341 cgacggccac gcaatgaatt gcgcccccgt catcggttat tagaggctgt ctgcccccgg
     8401 atacccggat tctttctgat gcctgacggt gacgatcaca atctggtcca tttcccggtc
     8461 gtggcggtag agcatcacat agccactgtc gccaaacccg atcaccagtt ccttatattc
     8521 cagcggcaga aacggcaccg ggcggccaat gtctggcaat gttttcagtt gctggatagc
     8581 ccggacgatc acctcacctc cttttctggc tgccagccga tttttggttt tgagaaagtc
     8641 ctgaaggcgt tgtaaatcgc gctgtgccag cgcggaaatc gttacctgtg gcatggcgga
     8701 gcatcctgct cgttctcagt tccccaggta cttatccagg cttccgcttc ttccgcagta
     8761 aggtgcagac ccgtttcctg atagtgctgc catgcggcct gtccgtcccg caggtactgg
     8821 ctgcgcttct cttcccggtc gatgtattcg gtgattgcct ccagcattag cgcgtgcgct
     8881 gagcggtgcc ggtcatcggc cagcgttttc agccggtcct taagttcttc atcgagcctg
     8941 atggatgttg ctgatgacat aaaagcccct ttgtagtcat atgtattaca catgactact
     9001 ttaatccgtg gcggcgctat ttgccagaaa tacggctttt gctgcccatt tttcagcttc
     9061 gcttccacta atcagggctg ctacaggctc actcatcatc caggactatt gcagcctgcg
     9121 ggttaaagga cctgttactg gcggcaacca tcatcattga gcttttgggt ggtgactggt
     9181 cgcggatcct gtaagccgtc atttttcaga ctgaattcaa aatttataac ctgtcgcatt
     9241 gccgggtgcc ctgcgggata tagccagtgc ctcattgccg tcagcgtttc ccgaaaaaaa
     9301 aggtgacagc cggtaagcga cacgttgtcc actcgtccat cgtccagaat atcaaacgtg
     9361 gcggtcacct gaccttcgat gcgttttgct gcggcctgta ccgggtaggt tggctgcagt
     9421 gtggttacgg gttttagtgg tatcgcatgt accggagtga ggaacatggc cgccagcact
     9481 gtcagagtaa aagctttcat ggtctgaaat ccagaggggc gtttttgaat atttgccagg
     9541 tattttcatc cggtgccggc ggaattttgg cgtgggtaac ggctgatatt gctgctttac
     9601 agagtttcgg atctcctcct tcagcaatgg cgctgttcag agtgccatca cgttgtatgg
     9661 atatccgcac ggtgcacatt tttcccgcat aggcctctgg ttcaaacatc tgcatttcaa
     9721 cagcggtctt gatggccgca gcgtagctac tgattttaga tgacttatcc agagtataaa
     9781 ttttcggcgg ctgggtggtg gaatgcttac aggccgtcag gagtaagggg ctgcacaatg
     9841 ccagcaggat aaagggtatt aaacgcatcg ggttgatgac ctgtcagtaa aaaatgttac
     9901 atgtatattt gtcactcttt acggccacgt tcatttacat aaacacggct tatcgtatcg
     9961 tttttacacc aaacggcccg ccatgcagtt gcgtgcatag gcatgcagcg ggttacaatt
    10021 taccgggcgc gtttccggcg gttttccggg tggtttgttg cctgttttat cccgtagccg
    10081 ccggaaacgc cctcagaccg tctgagcggt gcgggcaatg cgacgttatg gtaaataccc
    10141 cttaccggcg gcgctgtggc tgccaggtga aggtggtttc cgtagcctcg tatttctccc
    10201 gacgcatttc ctgcgcccgc ctggcttcac gttcgtcctg aatggcacga agcacccttt
    10261 cccgcacctg ttgttttacc gcctttggca gcgttttgtt ccctttcagg cccatggcca
    10321 gtgcctgagc gacaaaggcg tccgccaggc gcttttcttc agcgggagtc agtttcaggt
    10381 gcgtcatagt ggcctccggg cgtctcaggt ttcccgaaca gtttaaccgc aggtcctctc
    10441 ctcatgccag ttagctttca gatgtgtcta taccatctga aatgtaagtg tgatgaatgt
    10501 cttaaataat aaaatttgtt gccatgagaa attttcggga aaagacgcga tctgcttaca
    10561 ggtggctccg ggcgcttttt tgtgcctgaa gatgagaact aatctcatat attgacacct
    10621 gaaaacggga ggagtaaggt aataattata ctgtgtatat gtacagtgtg cgccgggaga
    10681 ccggttagag atcaaggggt gaaagtcccc gaccattgaa ggaccagcaa tccacaaggt
    10741 ccccgagtca tgcgttgcat accgcgaggt atggggcgaa gcgttgacag gggtgttgac
    10801 aggccagcca ttgagccacg aaatgtatat taaattaccg ggtgccgacg ttgtactgtt
    10861 aacggaaggc aacatcatag ggtgcgatac tgcgagtgcc acatggaccc ggcggggtct
    10921 gagaccctgg catgtcaata cgatctctac gcgggaaccg ggagatctcc cctctgacca
    10981 tctgccagtg tcggagatgg cccgcaccgg gaagacgagg agtcatagcc ggtgatgtac
    11041 ggagaggaga agtcggactc gctcatagta gcggcgaagc aggcgaacaa cccgaaagga
    11101 gcggagtcag tggagcgaag gagcggggcc aaggggaacg cggaacagcc acacatgcgc
    11161 cggacacaga gccgggaaag catgtcacag aggctgtcac gcgtgcggga agctgcgaag
    11221 cagcggaaga aagaacggtt tacagcattg ttccacctgc tgacagtcga agcactggaa
    11281 gccgcattcc tctccctgag caggaaagcg gccgccggag tggatggcat caggtggatg
    11341 gactacgccg gaaacatgaa gaacaacata acagatctgc accggaggct acatcagggc
    11401 agctacaggg cgcagcccgg caggcgtcac tacatcccaa aagcggatgg aaaacaacgc
    11461 ccgctcggca tcgcctcgct ggaggacaag atcgtccagt atgcgctggt gaaaatcctg
    11521 aacgcagtct atgaaaacga ctttatgggg ttctcatacg ggttcagacc cgggcgaagc
    11581 cagcacgatg cactggacgc actggccaca gggctggtac gcactaacgt aaactgggta
    11641 ctggatgccg acatcagtca gttcttcgac agggtgagcc acgaatggct gatcaggttc
    11701 acagagcatc ggatcggcga ccggagggta atcaggctca tacgtaagtg gctcacagcc
    11761 gggacgtcgg aggagggtca atggcgagca acggaggaag gcaccccaca gggtgcggtc
    11821 atctcaccgc tgctggcaaa catatacctc cactacgtct tcgatctgtg ggcgcatcag
    11881 tggcgacgtc gctatgccac aggcaatgtg gtaatggtca gatacgccga tgacatcgtc
    11941 atcgggttcg acaaacgata cgatgcccgg cg
//
