LOCUS       NZ_CP088992             5596 bp    DNA     circular CON 12-FEB-2024
DEFINITION  Klebsiella pneumoniae strain 50700 plasmid p50700-5.596, complete
            sequence.
ACCESSION   NZ_CP088992
VERSION     NZ_CP088992.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23552370
            Assembly: GCF_021166115.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Chen,L., Hao,M., Lin,Y. and Kreiswirth,B.
  TITLE     Whole genome sequencing of K. pneumoniae isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Chen,L., Hao,M., Lin,Y. and Kreiswirth,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 111 Ideation Way, Nutley, NJ 07110,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-SEP-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/12/2024 03:19:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,968
            CDSs (total)                      :: 5,849
            Genes (coding)                    :: 5,656
            CDSs (with protein)               :: 5,656
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 193
            CDSs (without protein)            :: 193
            Pseudo Genes (ambiguous residues) :: 0 of 193
            Pseudo Genes (frameshifted)       :: 89 of 193
            Pseudo Genes (incomplete)         :: 115 of 193
            Pseudo Genes (internal stop)      :: 22 of 193
            Pseudo Genes (multiple problems)  :: 33 of 193
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP088992.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="50700"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p50700-5.596"
     gene            complement(join(5542..5596,1..455))
                     /locus_tag="LRZ81_RS27035"
                     /old_locus_tag="LRZ81_27035"
     CDS             complement(join(5542..5596,1..455))
                     /locus_tag="LRZ81_RS27035"
                     /old_locus_tag="LRZ81_27035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            706..951
                     /locus_tag="LRZ81_RS27040"
                     /old_locus_tag="LRZ81_27040"
     CDS             706..951
                     /locus_tag="LRZ81_RS27040"
                     /old_locus_tag="LRZ81_27040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     gene            complement(1133..1477)
                     /locus_tag="LRZ81_RS27045"
                     /old_locus_tag="LRZ81_27045"
     CDS             complement(1133..1477)
                     /locus_tag="LRZ81_RS27045"
                     /old_locus_tag="LRZ81_27045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1467..1643)
                     /locus_tag="LRZ81_RS27050"
                     /old_locus_tag="LRZ81_27050"
     CDS             complement(1467..1643)
                     /locus_tag="LRZ81_RS27050"
                     /old_locus_tag="LRZ81_27050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2283..2441
                     /locus_tag="LRZ81_RS29855"
     CDS             2283..2441
                     /locus_tag="LRZ81_RS29855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            2799..3125
                     /locus_tag="LRZ81_RS27055"
                     /old_locus_tag="LRZ81_27055"
     CDS             2799..3125
                     /locus_tag="LRZ81_RS27055"
                     /old_locus_tag="LRZ81_27055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            3151..3696
                     /locus_tag="LRZ81_RS27060"
                     /old_locus_tag="LRZ81_27060"
     CDS             3151..3696
                     /locus_tag="LRZ81_RS27060"
                     /old_locus_tag="LRZ81_27060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            3762..4112
                     /locus_tag="LRZ81_RS27065"
                     /old_locus_tag="LRZ81_27065"
     CDS             3762..4112
                     /locus_tag="LRZ81_RS27065"
                     /old_locus_tag="LRZ81_27065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(4182..4448)
                     /locus_tag="LRZ81_RS27070"
                     /old_locus_tag="LRZ81_27070"
     CDS             complement(4182..4448)
                     /locus_tag="LRZ81_RS27070"
                     /old_locus_tag="LRZ81_27070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4432..4656)
                     /locus_tag="LRZ81_RS27075"
                     /old_locus_tag="LRZ81_27075"
     CDS             complement(4432..4656)
                     /locus_tag="LRZ81_RS27075"
                     /old_locus_tag="LRZ81_27075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(5323..5535)
                     /locus_tag="LRZ81_RS27080"
                     /old_locus_tag="LRZ81_27080"
     CDS             complement(5323..5535)
                     /locus_tag="LRZ81_RS27080"
                     /old_locus_tag="LRZ81_27080"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1730..1806)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          1995..2392
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(2..1143)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.738"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 cagctctgcg tcctgtctac ggccgtcccc ttcggcagca ccagaaaacg tccgttgctg
       61 tcttccaggt acgtcacgcc ccaggtcttc gcgttcagct tcgacagcgc tgcgttctgc
      121 cggtcgattt cggccagatt tgacgctatc aagctcccct gataccacaa caccccgctc
      181 aggctggcga acagcagtcc tgacaccatc gtgatggtca gccatgtccg cccgaccata
      241 cgcatcacgc tcaggcggtt cgactgcatg gccgcgctca taccctgctc gtgggcgcgg
      301 atggcggcgc tgattctctt cccgctttcg ctcagttccg ctctgacaga cttctcgtgc
      361 tcgctgaatg cggctttcag catttcgccg gtactctgct gctgcgcttt cgatttctgc
      421 tctaagtcct tcgccagcgt taaaagactg ttcatagatg gctcccttaa gtcggatgtt
      481 tcgccctccg tccgggtcgg caatgctgat gctgctcttt gtggtgcgca cgacctcaaa
      541 acctgcgctt tccagcgcct cagtgacgtc ctgacgcgtt ttaagctccc ctgacgaggc
      601 gagggccagt aagccccgcg taatagcctc tgcggcttcc tgcttcgttt tgggtaacga
      661 agatggcgtc accagcgcgc gccggttctc cggtgcgttc gggtcgtgca gccccagtcg
      721 cccgttcacc acggtctgcc acgcatcgat gcgcggacgg tctgcccggt cgtagtacgg
      781 ctggagacgc ctgccggtca gcagttccgt gttcggtacc agaaaattca gttccagccg
      841 ccccttgtcc cggtgttcaa cccacagcac gctgtactgg tctttatcga gtccgggcat
      901 cagaacccgc tcgaagctcg ccatcagctt ttcacgctgg ccgggcggta aatcctgttc
      961 agcaaaagac aggacgccgg aggtgtactt tttggcgtag ggcgaggcgt cgataagctc
     1021 ccggacctcc tccggctttc cctgcaggac gatggccccg tcgcgctggc ggtctttccc
     1081 cagcagataa tccaccggcc cggcaccgcc gcctcgccct cgcggatgaa acttaacgat
     1141 catcgtctgc ccccttttcc agcacggcat gccgcagccg ctcgagttcg gcatcaatgg
     1201 ccatcagcgc ggccaccacc tgaacccggt cagcgcctga ccactggccg ccgttaattt
     1261 tccgggcgat ctggttgagg ttgttcccca tcccggcaag ctggcgcagc aaaaccggat
     1321 cgatggacgg cagccgcaac gaccgcgccg ggcgcgtatc gagacaggtt tcccgcatcc
     1381 acaccgccag ttgccttcca ttgcagcgct ccagcagctg ctggtactcc ccatcggtca
     1441 cccggatacc taaaaaacgg gtacgcttaa ccgccatcct ctcctccgat atccgcaaaa
     1501 tgtgaccggg ctgaacgctc aagctctgcc atctgctccc ggcggtaatc ttcctcctgt
     1561 gcctgacgca tcagatttgc catcactgcc ggatgccagg atcttttttt tacgacgagc
     1621 ggcttcttct tcggcttctg cattatccct ccttcgcaaa cgccggacgg cacggaaaag
     1681 ttttccgggt cgctccggcg ttggacaagc gccagcgcgt cagtggtgtt agcgggtttc
     1741 ggggcgcagc cctgaaccag tcacagcgtt tacgctgtgt atactggctt actatgttgg
     1801 catgagagca cgattcatga aagtgcctca tgtggcaggt gtgatatgtg gctcaggagc
     1861 gtccgcaata ccatgcacgg acagatgaaa tgacgcctcc tcgctcactc ggtagctgcg
     1921 ctcctgccgt tcggctgcgg cgggcgtgac cgactcacgg ataacgggat aaagcaaagg
     1981 cgctcaaatc agaaagggcc ggagaaaagg atgcggcgtt gccgtttttc cataggctcc
     2041 gcctcccctg acgagcatca cagaaatgga cgctcaagtc agaggaggac gaaacccgac
     2101 aggacttaaa gatgccggtg atttctctcc ggcggctccc tcgtgcgctc tcctgttccg
     2161 accctgccgt ttaccggata cctgttccgc ctttttccct ttcgggaagc gtggcgcttt
     2221 ctcatagctc acgctgctgg tatctcggct cggtttaggt cgttcgctcc aagctgggct
     2281 gtatgcacga accccccgtt cagcccgact gctgcgccct ttccggtaac tatcatcttg
     2341 agtccaaccc ggacggacac gcaaaaacgc cactggcagc agccattggt aactggatat
     2401 atacgagaga gatattgaga ggtcttgaag tgggtgccta actgcggcta cactgaaaga
     2461 acagttttgg tatctgcggt ctcgtatacc cagttacccg gttaagcagt tccccaactg
     2521 acttaacctt cgatcaaacc gcctccccag gcggtttttt cgtttacagt gcaagagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagat cctttaaatg atcgtcagtt ctgttatccc
     2641 cggagctcca tccatcccac ggaaaaacac ccgccactga tgccctcagc gcactgcaca
     2701 tcatgtacaa accactccct ccctcctttt ttattgcgtt tcttaatgcg caatggtagc
     2761 tttgtgcgca acaaaaaacg caatagcaag ggggggtaat gagagaaaga acaaatatca
     2821 tgctagatat aggattaaag caatccttac aacggctttc gaataaaacc aaaatgagtc
     2881 taagtgatct tattaaccag agattatcag gttcactttc agttgaagaa cgcattggcg
     2941 aaaagcctga atggatgaga aaccttgatc cctatctcgc caatcacttt tcaaatattg
     3001 agttaccgct cccttcacca ttaaaagaat accaagaaaa aatggatgag atagattcaa
     3061 ttattaatga catgatggtt ctaaaggaat atttcagagc taagttttac gacgaaataa
     3121 aataggcacg ctatacggag gcggagaatt atgtttttta aacggtattt aacggagaag
     3181 gaaatataca aggcagcact cccaattgtt aggcttcaag ggagagttaa cacttcatta
     3241 cgttttttat ccaaatactc agatgtaaac aaaaatggat taattgcatt gcatcttaat
     3301 ttattacatt ttatctcata caacatacac ggtgtagagt ctctgaaaat cactcaagca
     3361 ggcataaaca aagctgtaca acaaagccct cacgcagatg aaataaaaaa tattgcgcag
     3421 gcaataggac gcaaaggacc tgagtacatc gaaggattaa ttagaaaccc atgggggaag
     3481 aaaaatgata atccactaat atttataatg agcattgaag aaagtataaa agaaaccctt
     3541 aactcaggtt atatgttata tagagtagct cccataactg agggtgaagc agctgctttc
     3601 atggatgttc ttaaacaatt attattattt gaggataatt ttcgcctttt tggtagagac
     3661 ttggtttata aaatagaaaa attaaaaatc agataacaaa gtatggtgaa cgaaaaatga
     3721 aagagaggat agtgaaatgt gtaataccaa gattctcaac tatgtattgg ttaattgcgt
     3781 tcctagcatt tatattcccc ggtcgatctt ttgctgacgg ttacaccgct tactaccgat
     3841 gtaatggtac tcaggaagca tctcttactg ttaaagataa caaactaagt atggttgtag
     3901 gtctttctaa gtttaatagg caactggata cttatagatt caaagaaaag ggaatcgaag
     3961 tagtaatgca gagagctgct tctgatgata actctgaggc cataatgtct tttattatgc
     4021 ctgacgttaa caatatgacc ttattagtta aaaataacgg ctacattatc gttaagacaa
     4081 cctgctggtt aactaaaacc gacaaacagt agaacacgta gttagtcgag tatttaccat
     4141 aatacctgtt gtcggcatca aaaaagcccg catagcgggc ttcagtcgta aacttcgcgg
     4201 cgatgtccaa tcgtggcggc aaggataaca agctcgtcat ctcggatctc acacagaacg
     4261 cgataatctc ccacccgata gcgccagaac tcgcccagct cacctttgag cggcttccct
     4321 gactggcgag gatcggcagc aactgcaatg cgtaaactca taaaatccac aatccgtcgt
     4381 gcgttctgtt tgtccatctt gcgtaacgat ttgagcgccc gatcggaata gttaatcgtc
     4441 cagaccaagt gacttcctca cgtcttcaga cgaatgcact gcttcatcac cacggcgaac
     4501 gcgtgcagca gtttctgctg aaagataata atcctccagg tcttccagat gcgccagtat
     4561 tgcttcccgc gcataaaacg tctttgttct gccggtttgc ttcgccagcg agtccagacg
     4621 ggactcaatc tcatcagata accggatagc aagcatataa acctcccgaa caccatttgc
     4681 tatacatgta tatcaacagt accacaatca acctaaaaga acaaaaaata atcttgttag
     4741 cccctcttga taatccgtaa cgcggtcaat atatctagct tgagggcttt cctcttaact
     4801 attgaaagga taacgattat gggtcttaag ccgggtccga aacgaattgc cgattcaaca
     4861 ggggaacctg ataaacgcca gcgtgacaat aaaaaaacgc cgggaaatac tgacaaattg
     4921 aaacccagca aatcttcaaa aaaataagtc agtagttaaa agccctccgg ggcttttttg
     4981 tatctgccgt tcacgctgat taaacataac acccgttgcc cgcaccgccc cggacgggtt
     5041 cgccgcgaac ggggtggcag tcaggtaaaa ccggcaacaa accacccgat aaaccgcccc
     5101 gttcgcgaca gataaatttt acctggctgc atagccatgc agccaggtga atgacatccg
     5161 gtttcagcgt aaatgcccga tgagaaccct gttttcctgc atttcagccc agcgacggac
     5221 cgtcatagct tcgctgccgg gaacgcgctt ccgtcagttc cttttcgtgc tgccgttgct
     5281 cgtgcgcccg ttctgcggcc acggccatct catgacgctg cactaccggc tcaaacgctg
     5341 cagttgcccg ctcaagcgtt ccacctgctg actcaagcgc gtcacgcgct cgctcagcgc
     5401 cgcgttctcc cgttgcataa gccctgacat tttccgccat tccccgaagg cgctctccca
     5461 ctcgtccagc cttttcgagt agtcctgctg tagctgctct aatgcgctca gcaactgttt
     5521 ttccagctcg gtcatcggca cttactcctt caccagtctc agcgcgttct gcttcgaccg
     5581 cccgttcccg accgtc
//
