LOCUS       NZ_CP088231             2495 bp    DNA     circular CON 29-MAY-2024
DEFINITION  Enterobacter kobei strain Ek72 plasmid pEk72-2, complete sequence.
ACCESSION   NZ_CP088231
VERSION     NZ_CP088231.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22612881
            Assembly: GCF_023547315.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Zhou,K.
  TITLE     Emergence of mcr and carbapenemase genes in clinical Enterobacter
            cloacae complex isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Zhou,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) Institute of respiratory diseases, Shenzhen
            People's Hospital, Dongmenbeilu, Shenzhen 518020, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 110.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023547315.1-RS_2024_05_29
            Annotation Date                   :: 05/29/2024 05:02:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,839
            CDSs (total)                      :: 4,724
            Genes (coding)                    :: 4,609
            CDSs (with protein)               :: 4,609
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 38 of 115
            Pseudo Genes (incomplete)         :: 78 of 115
            Pseudo Genes (internal stop)      :: 19 of 115
            Pseudo Genes (multiple problems)  :: 16 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP088231.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="Ek72"
                     /isolation_source="shunt fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:208224"
                     /plasmid="pEk72-2"
                     /geo_loc_name="China:Shenzhen"
                     /lat_lon="22.38 N 114.05 E"
                     /collection_date="2018"
                     /collected_by="Yang Ji"
     gene            1016..1234
                     /locus_tag="LK774_RS23805"
                     /old_locus_tag="LK774_23845"
     CDS             1016..1234
                     /locus_tag="LK774_RS23805"
                     /old_locus_tag="LK774_23845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182407767.1"
                     /translation="MFGFSSGALMTFNDDERHLLVSVVSGWLRRAEGDAGAMMLDAYRQ
                     ILSETEPAARAVMLEFLESVRIHYVSS"
                     /name="hypothetical protein"
     gene            complement(1532..1717)
                     /locus_tag="LK774_RS23810"
                     /old_locus_tag="LK774_23850"
     CDS             complement(1532..1717)
                     /locus_tag="LK774_RS23810"
                     /old_locus_tag="LK774_23850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032673404.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEKDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2083..2310)
                     /locus_tag="LK774_RS23815"
                     /old_locus_tag="LK774_23855"
     CDS             complement(2083..2310)
                     /locus_tag="LK774_RS23815"
                     /old_locus_tag="LK774_23855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          737..906
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.698"
                     /coverage="98.0"
                     /evalue="2.0600000000000002e-57"
     Typing          1398..1485
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.382"
                     /coverage="100.0"
                     /evalue="1.5000000000000001e-30"
     Typing          complement(230..812)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.331"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 atgatttaag tattcctctt tgtgaggttg gatgtctaat ttataaagga tcttcttgag
       61 atcctttttt ttcgcgcgta atctcttgcc ctgtaaacga aaaaaccacc ctggcaggtg
      121 gtttttcgaa ggttaggtaa tcctggcaga tcccctaacc gtggtaacag tcttgtgcga
      181 gacatgtcac caaatttgtc ctttcagtgt agcctcacta aggccgccac ttcaagaact
      241 cttgatacat ctctcgcaca tcctgtttac caatggccgt tgccaatggc gattagtcgt
      301 gtctttccgg gttggactca agttgatagt taccggataa ggcgcagcgg tcggactgaa
      361 cggggggttc gtgcatacag tccatcctgg agcgaactgc cttcccggaa ctgagtgtca
      421 ggcgtggaat gagaaaccgc ggccataaca gcggagtgac accggtaaac cgaaaggcag
      481 gaatgcgggg gagcacgagg gagccaccag ggggaaacgc ctggtatctt taagccgcat
      541 cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag
      601 cctatggaaa aacggctttg ccgtggcctt atgccttttt cgctaagaac cttcctggca
      661 ttctccagga aatttctgct ctgtacgtaa gcccgtaccg ctcgccgcag ccgaacgacc
      721 gagcgcagcg agtcagtgag cgaggaagcg gaatatctcc tgtatcacat attctgctga
      781 cgcaccgatg ccgccttttt ctcctgccac atgaagcact taactgacat ccgcatccgt
      841 gccaacatat taagccagta tacactccgc tagcgctgcg tgactggtcc ggcgctgcgc
      901 ccggaacccg cctgtaccgg ttaagcagcc gttctggtct gactgcgatt tttttcattc
      961 ctgcccgcta ccctgtaaac ctttcttctg cgttgccgtt aacaggaggt aagccatgtt
     1021 tggtttttcc tccggagccc tgatgacttt taatgatgat gagcgacacc tgcttgtgtc
     1081 cgttgtttcg ggctggctcc ggcgtgccga aggggatgcc ggagccatga tgctggatgc
     1141 ataccggcag attttgtctg aaaccgaacc ggccgcaagg gcggtcatgc tcgaatttct
     1201 tgaatcagtt cgcattcatt acgtttcgtc ctgagcattt cctgaagcat ttcctgaatt
     1261 tttgtccata ctcaatccag gcggccgtca cggacggtgg tcgtcgttcc atgatgctgt
     1321 tatggaagat tgagagtaat ctaacttgat tgagttccgg gcgtgtcgcc cggttttttt
     1381 tgctgtttcc cggtgtccgc tcccggacag gatgtgcatt tgtaataccg cgcacgcggc
     1441 ggcatcacaa ataccccgtt cagggctgcg ccccgacacc ccgccatcgc ctcacggccg
     1501 cgcactgaat tgcgcacccg ttcaccgatt ttcacgcatc tttctcgcct atctgtgcgt
     1561 tcagcatggc gtaaagcgct tcagcctgat cgtgcagatg ctcacagcag ccggctgcct
     1621 catccagatc catctgttcg agccgttcga ggaggaggag tgtctgtgag cggataaatt
     1681 tggccatgtt gagggcggcg gattcctgtc tggtcactgg tgattttccc cgtctggtga
     1741 tgatttttgc gtgaaaagag catgccgtca ttctggtgca tagtcatgca tgccgttaaa
     1801 atttaacagg cgcgtttctg acgggttccg gggtggtttg ttgtggtttt tgtcatggtt
     1861 ccgtcagaaa cgcgctgagc gcgtctgagg cggtgcgggc aacggatgtt atggtaaatt
     1921 cttgggggct tgattataaa ataccgcact tcctcttatg tcttgtgctt tttagacaac
     1981 cctgaaagct tttagctttg gtgatgcaat ttgctgggat tttggcggag aaccacaggt
     2041 aaagaaaaag gccacattag cggccttttt cggagaatat gctcagtgcg gaatttcgga
     2101 gacttcttta acgtcggttt tattgatctg ctgtttgaca ccattcgcgt ctgtatagcc
     2161 aagcaggcct gagtctgatt ctttaggttt gccgtcgctg acaatagtgc gaccgtcatt
     2221 ggtgtgtatg gcatagcttg tgcgcgtgca gccggtgagg gctgaaagcg caactgccgc
     2281 tgcgaacact gaaacaaaaa tctttttcaa agccagctcc ttttatccat atcagttttg
     2341 gtcactaacc atctaactat aggttatttt ttatgtgaaa agaggcttga tggtgggctg
     2401 tatatcgagt taggcgcgag gtgctatggt caaagtgtgg tgtcaagcgg ggaggaggct
     2461 tatatgagat gtaatccagc agatacatat accaa
//
