LOCUS       NZ_CP088225             4934 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain Eh60 plasmid pEh60-4, complete
            sequence.
ACCESSION   NZ_CP088225
VERSION     NZ_CP088225.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22612719
            Assembly: GCF_023547225.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Zhou,K.
  TITLE     Emergence of mcr and carbapenemase genes in clinical Enterobacter
            cloacae complex isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Zhou,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) Institute of respiratory diseases, Shenzhen
            People's Hospital, Dongmenbeilu, Shenzhen 518020, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 110.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023547225.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:55:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,160
            CDSs (total)                      :: 5,047
            Genes (coding)                    :: 4,897
            CDSs (with protein)               :: 4,897
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 49 of 150
            Pseudo Genes (incomplete)         :: 89 of 150
            Pseudo Genes (internal stop)      :: 30 of 150
            Pseudo Genes (multiple problems)  :: 16 of 150
            Pseudo Genes (short protein)      :: 1 of 150
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP088225.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eh60"
                     /isolation_source="bronchial"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pEh60-4"
                     /geo_loc_name="China:Shenzhen"
                     /lat_lon="22.38 N 114.05 E"
                     /collection_date="2017"
                     /collected_by="Yang Ji"
     gene            complement(40..1326)
                     /locus_tag="LK764_RS24595"
                     /old_locus_tag="LK764_24655"
     CDS             complement(40..1326)
                     /locus_tag="LK764_RS24595"
                     /old_locus_tag="LK764_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899670.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_223230178.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWERLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTRKGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            complement(2254..2469)
                     /locus_tag="LK764_RS24600"
                     /old_locus_tag="LK764_24660"
     CDS             complement(2254..2469)
                     /locus_tag="LK764_RS24600"
                     /old_locus_tag="LK764_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2476..2967)
                     /locus_tag="LK764_RS24605"
                     /old_locus_tag="LK764_24665"
     CDS             complement(2476..2967)
                     /locus_tag="LK764_RS24605"
                     /old_locus_tag="LK764_24665"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_047720453.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<3002..3706)
                     /locus_tag="LK764_RS25825"
                     /old_locus_tag="LK764_24670"
                     /pseudo=""
     CDS             complement(<3002..3706)
                     /locus_tag="LK764_RS25825"
                     /old_locus_tag="LK764_24670"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3645..3968)
                     /locus_tag="LK764_RS24615"
                     /old_locus_tag="LK764_24675"
     CDS             complement(3645..3968)
                     /locus_tag="LK764_RS24615"
                     /old_locus_tag="LK764_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_176490892.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRPLAAWMRQTCLDEKSARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4082..4254)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.276"
                     /coverage="100.0"
                     /evalue="9.21e-81"
     Typing          4179..4725
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.545"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(2157..3655)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.297"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tctgtgatgt tcgacagata tggggatatg caccatggcc tatccggctt taactgaaat
       61 ggcgacatcc ttaaccgcag tctcaacccc tgcagcattc ctctgaatgc tggtcataac
      121 ttcacccgct tcgcgcatga gtgtaacccc tttccgagtg ttcatcaggc tgttttcaat
      181 gccacagaga acatccttgg ccagttgact attgctggcc acgacttgct caatttcttc
      241 agtcgcgcta ctgacgctcg ccgcaagatt acgcacttct gcggcaacaa ccgcaaaact
      301 tcttccggat gcgccagccc ttgctgcttc aatcgcggca ttcagtgcaa taagtcttgt
      361 ctgcatggca aacttccgga tggtttccac catatcgtca atactttcag actgattgtt
      421 gagccgtgag atatcggtgg agacagcccc catcccctgc gcaattctgt caatcataag
      481 gatgctattt tcaataacat tggcaccgtt ctgcgcactt tctcgggtct gcacggccat
      541 atcccatgca tgcacagcag cctcttgctc tctctggttt cgcaggacat cagccgtaac
      601 atctgtagcg aatttcacaa ttttataaag ctgcccatca ctgttaaaga cagggttata
      661 agttgcacgg aaccacagag ggtctccctg acggttaagg cgtggaaaca gaccagaaaa
      721 aaactcacct tgattcagtc tctcccagaa atgtcggtat tcatcacttt tgtaaagcgt
      781 ctcggcacag aacagacgat gatgcttacc tatgatttca tcccgcttat agccagtggc
      841 attgacgaaa ttttcatttg cttcaagcac gatcccttca ggattgaagg tgatgatggc
      901 catagaacgg tttatggcat ttacgactgc ttcctgctca agagcagaat gcacgcgctc
      961 agaaatatcg gcagcaattt taatgactct gataacacgg ccgcgcctgt cagaaacggg
     1021 gatataactg gcttcaagcc atacggaacg atttcccttt gccagccgca ggtacttgcc
     1081 actaaagctt tctcccctgg caaggcgctg ccagaactgg ctgtattccg gtgaacttac
     1141 cagcgagggc gggcagaata tacgatggtg gtgaccgatt atttcatcag ccctgtaccc
     1201 catagtggag agaaaaagcg ggctggcttt atttatcacc ccctccggac tgaactcaat
     1261 gacaggctta ttctgacaaa ttgcgtcata cgaaatggct gatcgctgga ggttcaggga
     1321 gaacatatat ttaagtgaaa acatatgaat cagattgccc ggataatgta agtactgcca
     1381 tggttatcgg ctactcctta tgtagcttta tatattactg cgacagggcg ggaaaaagcc
     1441 cgctcttcag ccatgccggc atgatgtccg gctccggatt tcacgcgtaa aaaacccgcc
     1501 atctttagcg ggctgaacaa taagaaaatg ttcatattta cggcaaatcc gccggctcca
     1561 gcaggaagct ggcgtctctc aatcttggaa gaaccagcgc agctccgcaa gccttaacac
     1621 gcatcagaag gcaaaaaaat ggctcccatc cgggagacgg aaaacactca actgactgga
     1681 tcgcaggata tcctttgtga aggataattt aagtatcggc cggatttccg cgggcataag
     1741 cccggccggc ataatttttt ccgtatccgc cagacctcgg cttttaacat aacacccctt
     1801 gcccgcaccg cagaaacagg gttggctgcg tttccggtgc cagacgcgag aaagtggcga
     1861 cagacatgac gaaaaaacac caaaaacgca gccgctggat attacactga tgcatagtca
     1921 tgcagccaga tgcatgcagc gtaaagtcac actaaaatca gcgcgaacgc cttaaaaacc
     1981 ggcaaatgcc tctccggcaa ctcacacagc tccatcacca ggtaaaaaca gttcagcagg
     2041 gcttcaataa cgttgagaat tttcatcact tcctccggga taagtcacta ccattccggt
     2101 agtcagccta tttagcagga acgcgatgag cccttcttcg tgcgggctgt cacctcatct
     2161 tcggaccatc gaaactcttc tgcttctgtc ggagcgtcag ctccagctcg ttctgacgct
     2221 gatactccag tgcagccttc tcctcgatgg cattcagttc ccggacaagc ggcgcagctg
     2281 ctcgcttaaa ctcagcacct gcgtgctcaa gtccgacacg cgctcgttca gcaccgcgtt
     2341 ctcccgttgc ataagaccac acatgctccg ccattccgcg aaggcgctct cccattcgtc
     2401 cagcctttgc gagtagtcct gctgtagctg ctctaatgcg ctcagcaact gcttttccag
     2461 ctctgtcatt ttccgctact cctgtttcag gcggatccat gtcgtcccgt cgtaggggat
     2521 ggcttcggct tgcatccccg gcggcaggat aagaaacctc cggccgtcgc tgctctcctg
     2581 atagcgcact ccccacgtcc gggcggtcat tttcgccagg gtatcttcct gctgacgaag
     2641 atgcgtgtaa ttgtcggtta tctgctgccc ctgccaccac agaatgctcc cgctcgtggc
     2701 gatcagcagc gctgacacca tcaggatggt gagccatgtc ctcccggccg tcagcaacac
     2761 gctccgccgg tttttctcca tcgcctcgct catactctgc tcgtgggcgc tgatggcgtc
     2821 gctgattctc ctcgcgcttg cgttcagttc cgctctgaca gactgctcgt gctcgctgaa
     2881 tgcggctttc agcatctcgc cggtactctt ctgctgcgct ttcgattgct gctctaagtc
     2941 cttcgccagc gttaaaagac tgctcataga tggctccctt cagtcggatg tttcgccccc
     3001 cgtcagggtc ggcaatgctg atactgcttt tcgttgtacg caccacctca aaacctgcac
     3061 cttcaagagc ctcagtgacg tcctgacggc ttttcagctc cccggacgag gctaagctca
     3121 gcagtccacg ggtgatggct tctgcggctt cctgcttcgt tttcggcagt ccggccggcg
     3181 tgaccagcgc ccggcggttt tccggcgcgt tcggatcgtg cagccccagt ctgccgttca
     3241 cgatggtctg ccaggcatcg atacgcggcc ggtcggcgcg gtcgtagtac ggctggagcc
     3301 gcctgccggt cagcagctcg gtattgggga tcagaaagtt cagctccaga cgccccttgt
     3361 cctcgtgctc cacccacaga atgctgtact gatctttatc aagtccgggc atcagaaccc
     3421 gctcgaagct ggccatcagt ttttcccgct ggccgggagg cagatcggcc tccgcaaacg
     3481 acaggacgcc ggaggtgtat ttcttcacgt aaggcgacgc gtcaatcagc tcccggactt
     3541 cttccggctt cccctgcagg acgctggcgc cttcacgctg ccggtcttta cccagcagat
     3601 agtccaccgg cccggcgcca ccgccgcggc cacgtggatg aaatttaacg atcatcgtcc
     3661 gttcctttct caagaacggc gtgccgcagc cgctcaagcc cggcatcgat ggccatcagc
     3721 gccgccacaa tctgcaccct gtcatggccg gtgccgccgc cggcgttaac acggcgggct
     3781 atctggttca ggttattccc catgccggca agctggcgca gcagcgccgg cgagattgag
     3841 gggagctttc cggtgcgcgc agacttttcg tccaggcagg tctgccgcat ccacgccgcc
     3901 agcggcctgc cgtcacagcg ctcaagcagc cgccggtgct cgtcctccgt cacccacatg
     3961 gtgagcatct tgttccgttt ctctgacaaa ccggcctcct gataacgccg ggacgggcgg
     4021 gaaagccttt cccggtcggc gcggggttgg acaagccgca ggcgcgtcag gcttttggcg
     4081 ggtttcgggg cgcagccctg aaccagtcac gtagcgctag cggagtgtat actggcttac
     4141 tatattggca cggatgcgaa tatcagtgaa gtgcttcatc tggcaggaga aaaatgcagc
     4201 agcggagcgt cagcagaata tgtgatacag gatatattcc gcttcctcgc tcactgactc
     4261 gctgcgctcg gtcgttcggc tgcggcgagc ggtaccggct tacaggcggg gcggaaattt
     4321 cctgggagat gccagaaaga agcttaacag ggaagcgaga aggccacgga gaagccgttt
     4381 ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg
     4441 cgaaacccga caggactata aagataccag gcgtttcccc ctggtggctc cctcgtgcgc
     4501 tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct
     4561 cattccacgc ctgacactca gttccgggaa ggcagttcgc tccaagctgg actgtatgca
     4621 cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcaac ttgagtccaa
     4681 tccggaaaaa cacgacaaag cgccactggc agcagccact ggtaacaggt ttagaggagg
     4741 agagtcttga agtttcgggt gcgataaggc tacactgaaa ggacagtttt ggtgactgtg
     4801 ctcctccaag ccagttaccg cggttcaaaa gagcgtcaac tcttgcgaac cttcgaaaaa
     4861 gccaccttgc cgggtggttt tttcgttttc agagcaagag attacgacgc aacggaaagg
     4921 atctcaagaa gatc
//
