LOCUS       NZ_CP088197            20449 bp    DNA     circular CON 21-MAY-2024
DEFINITION  Enterococcus faecium strain S39-7a plasmid pS39-7a-b, complete
            sequence.
ACCESSION   NZ_CP088197
VERSION     NZ_CP088197.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23424486
            Assembly: GCF_023375225.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20449)
  AUTHORS   Biggel,M.
  TITLE     Characteristics of florfenicol resistant enterococci from fattening
            pigs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20449)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 125x,185x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore Minion
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023375225.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 04:11:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,620
            CDSs (total)                      :: 2,529
            Genes (coding)                    :: 2,472
            CDSs (with protein)               :: 2,472
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 21 of 57
            Pseudo Genes (incomplete)         :: 34 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP088197.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="S39-7a"
                     /isolation_source="fecal sample"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1352"
                     /plasmid="pS39-7a-b"
                     /collection_date="2021"
                     /collected_by="ILS, University of Zurich"
     gene            join(19311..20449,1..238)
                     /gene="tet(L)"
                     /locus_tag="LQ043_RS12925"
                     /old_locus_tag="LQ043_12925"
     ARG             3..238
                     /name="tet(L)"
                     /gene="tet(L)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="17.03"
     gene            802..2064
                     /gene="mobV"
                     /locus_tag="LQ043_RS12930"
                     /old_locus_tag="LQ043_12930"
     CDS             802..2064
                     /gene="mobV"
                     /locus_tag="LQ043_RS12930"
                     /old_locus_tag="LQ043_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000119405.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYD
                     LKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFE
                     ESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKF
                     PEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVK
                     SDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDND
                     RLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSK
                     ISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNE
                     FELTHNREVKKERSRDQGMSL"
                     /name="mobV"
     gene            2309..>2761
                     /locus_tag="LQ043_RS12935"
                     /old_locus_tag="LQ043_12935"
                     /pseudo=""
     CDS             2309..>2761
                     /locus_tag="LQ043_RS12935"
                     /old_locus_tag="LQ043_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            complement(2816..3496)
                     /locus_tag="LQ043_RS12940"
                     /old_locus_tag="LQ043_12940"
     CDS             complement(2816..3496)
                     /locus_tag="LQ043_RS12940"
                     /old_locus_tag="LQ043_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(3929..4141)
                     /locus_tag="LQ043_RS12945"
                     /old_locus_tag="LQ043_12945"
     CDS             complement(3929..4141)
                     /locus_tag="LQ043_RS12945"
                     /old_locus_tag="LQ043_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002324171.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIEY
                     RTEYLELTNQTDFLLIRKQQEQEKN"
                     /name="hypothetical protein"
     gene            complement(4162..5013)
                     /locus_tag="LQ043_RS12950"
                     /old_locus_tag="LQ043_12950"
     CDS             complement(4162..5013)
                     /locus_tag="LQ043_RS12950"
                     /old_locus_tag="LQ043_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002287227.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILDL
                     TVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAGKL
                     FKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILFFVG
                     YVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKGIRSI
                     LKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDLELPMK
                     DK"
                     /name="hypothetical protein"
     gene            5196..6155
                     /locus_tag="LQ043_RS12955"
                     /old_locus_tag="LQ043_12955"
     CDS             5196..6155
                     /locus_tag="LQ043_RS12955"
                     /old_locus_tag="LQ043_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="WP_000222573.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHCLNRSRQTIHKVYLFF
                     KQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIRCS
                     ARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFKSITF
                     DCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDESF
                     IQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
                     /name="IS30 family transposase"
     gene            complement(6179..6475)
                     /locus_tag="LQ043_RS12960"
                     /old_locus_tag="LQ043_12960"
     CDS             complement(6179..6475)
                     /locus_tag="LQ043_RS12960"
                     /old_locus_tag="LQ043_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication control protein PrgN"
                     /protein_id="WP_000053907.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTRE
                     KTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
                     /name="replication control protein PrgN"
     gene            complement(6610..8103)
                     /locus_tag="LQ043_RS12965"
                     /old_locus_tag="LQ043_12965"
     CDS             complement(6610..8103)
                     /locus_tag="LQ043_RS12965"
                     /old_locus_tag="LQ043_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_000947691.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSKQ
                     LMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINTFV
                     VDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVIDVA
                     KKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSMKLES
                     DQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTLSLAYY
                     SSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELCQQWVSP
                     TLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLNLSKKELA
                     SQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLSKEKRLDYS
                     TRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
                     /name="primase C-terminal domain-containing protein"
     gene            8715..9668
                     /locus_tag="LQ043_RS12970"
                     /old_locus_tag="LQ043_12970"
     CDS             8715..9668
                     /locus_tag="LQ043_RS12970"
                     /old_locus_tag="LQ043_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000429439.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLFE
                     KDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGSPG
                     SEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAADYII
                     AMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQKTID
                     KYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYYEQHGD
                     IEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
                     /name="ParA family protein"
     gene            9640..9915
                     /gene="prgO"
                     /locus_tag="LQ043_RS12975"
                     /old_locus_tag="LQ043_12975"
     CDS             9640..9915
                     /gene="prgO"
                     /locus_tag="LQ043_RS12975"
                     /old_locus_tag="LQ043_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="WP_001196543.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVDP
                     PVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
                     /name="prgO"
     gene            10108..10362
                     /locus_tag="LQ043_RS12980"
                     /old_locus_tag="LQ043_12980"
     CDS             10108..10362
                     /locus_tag="LQ043_RS12980"
                     /old_locus_tag="LQ043_12980"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000199136.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYLP
                     DQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
                     /name="transposase"
     gene            10768..11930
                     /locus_tag="LQ043_RS12985"
                     /old_locus_tag="LQ043_12985"
     CDS             join(10768..11044,11044..11930)
                     /locus_tag="LQ043_RS12985"
                     /old_locus_tag="LQ043_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_086953888.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKWV
                     KQFKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANKHL
                     YPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSKRGI
                     QISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIVTDLT
                     YVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGK
                     EFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLEL
                     FDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
                     /name="IS3 family transposase"
     gene            12106..12603
                     /locus_tag="LQ043_RS12990"
                     /old_locus_tag="LQ043_12990"
     CDS             12106..12603
                     /locus_tag="LQ043_RS12990"
                     /old_locus_tag="LQ043_12990"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_228780308.1"
                     /translation="MCEKWAADITYIPTKKNGWCYLSSIMDLHTKRIISYTFSKRMTVD
                     CVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGI
                     ESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEK
                     MA"
                     /name="IS3 family transposase"
     gene            complement(13076..13648)
                     /locus_tag="LQ043_RS12995"
                     /old_locus_tag="LQ043_12995"
     CDS             complement(13076..13648)
                     /locus_tag="LQ043_RS12995"
                     /old_locus_tag="LQ043_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000170424.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRDR
                     PKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTSMG
                     RMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELLTSG
                     KSYKEVEAITGFSRSTLFRIKKKIEES"
                     /name="recombinase family protein"
     gene            complement(13664..14269)
                     /locus_tag="LQ043_RS13000"
                     /old_locus_tag="LQ043_13000"
     CDS             complement(13664..14269)
                     /locus_tag="LQ043_RS13000"
                     /old_locus_tag="LQ043_13000"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="WP_000599739.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTFK
                     GLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEIIKK
                     YVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDLEIR
                     YLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
                     /name="protein adenylyltransferase Fic"
     gene            complement(14467..15147)
                     /locus_tag="LQ043_RS13005"
                     /old_locus_tag="LQ043_13005"
     CDS             complement(14467..15147)
                     /locus_tag="LQ043_RS13005"
                     /old_locus_tag="LQ043_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            <15179..15892
                     /locus_tag="LQ043_RS13010"
                     /old_locus_tag="LQ043_13010"
                     /pseudo=""
     CDS             <15179..15892
                     /locus_tag="LQ043_RS13010"
                     /old_locus_tag="LQ043_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /name="C40 family peptidase"
     gene            15889..16821
                     /locus_tag="LQ043_RS13015"
                     /old_locus_tag="LQ043_13015"
     CDS             15889..16821
                     /locus_tag="LQ043_RS13015"
                     /old_locus_tag="LQ043_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="WP_001224319.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVLL
                     GLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSIDN
                     RMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQLITE
                     GENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSITTNEI
                     NEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTVSLTVE
                     YIDQQTKATQVSQFDLVLEKNGSNWKIVK"
                     /name="conjugal transfer protein"
     gene            17066..17182
                     /locus_tag="LQ043_RS13020"
                     /old_locus_tag="LQ043_13020"
     CDS             17066..17182
                     /locus_tag="LQ043_RS13020"
                     /old_locus_tag="LQ043_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="WP_001791010.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
                     /name="tetracycline resistance determinant leader peptide"
     gene            17198..19117
                     /gene="tet(M)"
                     /locus_tag="LQ043_RS13025"
                     /old_locus_tag="LQ043_13025"
     ARG             17199..19114
                     /name="tet(M)"
                     /gene="tet(M)"
                     /gene_name="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             19402..20447
                     /name="tet(45)"
                     /gene="tet(45)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="77.36"
                     /coverage="76.2"
     Typing          complement(6611..8103)
                     /name="rep_cluster_185"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          803..2061
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.19"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggaagctggt gctggaatga gtttgcttaa ctttaccagc tttttatcag agggaacagg
       61 tattgcaatt gtaggtggtt tattatccat acccttactt gatcaaaggt tgttacctat
      121 ggaagttgat cagtcaactt atctgtatag taatttgtta ttactttttt caggaatcat
      181 tgtcattagt tggctggtta ccttgaatgt atataaacat tctcaaaggg atttctaaat
      241 cgttaaggga tcaactttgg gagagagttc aaaattgatc ctttttttat aacaggaatt
      301 caaatctttt tgttccatta aagggcgcga ttgctgaata aaagatacga gagacctctc
      361 ttgtatcttt tttattttga gtggttttgt ccgttacact agaaaaccga aagacaataa
      421 aaattttatt cttgctgagt ctggctttcg gtaagctaga caaaacggac aaaataaaaa
      481 ttggcaaggg tttaaaggtg gagatttttt gagtgatctt ctcaaaaaat actacctgtc
      541 ccttgctgat ttttaaacga gcacgagagc aaaacccccc tttgctgagg tggcagaggg
      601 caggtttttt tgtttctttt ttctcgtaaa aaaaagaaag gtcttaaagg ttttatggtt
      661 ttggtcggca ctgccgacag cctcgcagag cacacacttt atgaatataa agtatagtgt
      721 gttatacttt acttggaagt ggttgccgga aagagcgaaa atgcctcaca tttgtgccac
      781 ctaaaaagga gcgatttaca tatgagttat gcagtttgta gaatgcaaaa agtgaaatca
      841 gctggactaa aaggcatgca atttcataat caaagagagc gaaaaagtag aacgaatgat
      901 gatattgacc atgagcgaac acgtgaaaat tatgatttga aaaatgataa aaatattgat
      961 tacaacgaac gtgtcaaaga aattattgaa tcacaaaaaa caggtacaag aaaaacgagg
     1021 aaagatgctg ttcttgtaaa tgagttgcta gtaacatctg accgagattt ttttgagcaa
     1081 ctggatccag gagaacaaaa acgatttttt gaggaaagtt ataaattatt ttccgaacga
     1141 tatggcaagc aaaatattgc ttatgcaaca gttcataatg atgagcaaac ccctcacatg
     1201 catttaggtg ttgtgcctat gcgtgatgga aaactgcaag gaaaaaatgt gtttaatcgt
     1261 caagaactgt tatggctaca agataaattc cccgagcata tgaaaaaaca gggttttgag
     1321 ttgaagcgtg gtgaacgtgg ctctgaccgt aaacatattg agacagctaa atttaaaaaa
     1381 caaactttgg aaaaagagat tgattttcta gaaaaaaatt tagcagttaa aaaagatgaa
     1441 tggactgctt atagcgataa agttaaatca gatttagaag taccagcgaa acgacacatg
     1501 aaaagtgttg aagtgccaac gggtgaaaag tccatgtttg gtttgggaaa agaaataatg
     1561 aaaacagaaa agaaaccaac caaaaatgtt gttatatcgg agcgtgatta taaaaactta
     1621 gtgactgctg cgagagataa cgataggtta aaacagcatg ttagaaatct catgagtact
     1681 gatatggcga gagaatataa aaaattaagt aaagaacatg ggcaagttaa agaaaaatat
     1741 agtggtcttg tagagcgatt taatgaaaat gtaaatgatt ataatgagtt gcttgaagaa
     1801 aacaagtctt taaagtctaa aataagcgat ttaaagcgtg atgtgagttt aatctatgaa
     1861 agcactaagg aattccttaa ggaacgtaca gacggcttaa aagcctttaa aaacgttttt
     1921 aaggggtttg tagacaaggt aaaggataaa acagcacaat tccaagaaaa acacgattta
     1981 gaacctaaaa agaacgaatt tgaactaact cataaccgag aggtaaaaaa agaacgaagt
     2041 cgagatcagg gaatgagttt ataaaataaa aaaagcacct gaaaaggtgt ctttttttga
     2101 tggttttgaa cttgttcttt cttatcttga tacatataga aataacgtca tttttatttt
     2161 agttgctgaa aggtgcgttg aagtgttggt atgtatgtgt tttaaagtat tgaaaaccct
     2221 taaaattggt tgcacagaaa aaccccatct gttaaagtta taagtgacca aacaaataac
     2281 taaatagatg ggggtttctt ttaatattat gtgtcctaat agtagcattt attcagatga
     2341 aaaatcaagg gttttagtgg acaagacaaa aagtggaaaa gtgagaccat ggagagaaaa
     2401 gaaaatcgct aatgttgatt actttgaact tctgcatatt cttgaattta aaaaggctga
     2461 aagagtaaaa gattgtgctg aaatattaga gtataaacaa aatcgtgaaa caggcgaaag
     2521 aaagttgtat cgagtgtggt tttgtaaatc caggctttgt ccaatgtgca actggaggag
     2581 agcaatgaaa catggcattc agtcacaaaa ggttgttgct gaagttatta aacaaaagcc
     2641 aacagttcgt tggttgtttc tcacattaac agttaaaaat gtttatgatg gcgaagaatt
     2701 aaataagagt ttgtcagata tggctcaagg atttcgccga atgatgcaat ataaaaaaat
     2761 tggttctgtt gcaaagtttt aaataaagaa taaaatccct tacggtatct atgatttaag
     2821 ctgggattcc caataatacc ttgatttcag tacagaccga aaacccgaag agagtgcctt
     2881 cttttcgggt tttcttatat aatcctcgaa tggcttccat gcctttaatc gtggtagagg
     2941 cagtgcgtaa acttcgatag aatttattgc gtctctttac tggacgatgg tcttgttcaa
     3001 tcaaattatt caggtattta atggtacgat gttctgtccc ttgataaaag ccgtattctt
     3061 ttagtttctt aaaggcactt gtaatagagg gggctttatc tgtgactaca accttcggtt
     3121 catcaaactg cttcactaac cgcttaagaa aagcataggc tgcttgtgtg tcccgttttt
     3181 tacgtaacca aatatccaag gttaaaccat ctgcatcgat ggctcgatac aaataatgcc
     3241 attttccttt aattttgatg tacgtttcat ccattttcca tgaataaaag gattttttat
     3301 ttttcttttt ccaaatttga tagagtagtt tgccatattc ttgcacccaa cgataaatcg
     3361 tcgtatgaga aacgttaatg ccacgatcat ataagatttc ttgaacttca cgatagctaa
     3421 ggttataacg aagatagtag cccacggcta caataatcac atcctgctga aattgctttc
     3481 ctttaaaatg attcatcgtc attcctcctg ccatcttttt ctattattct accttatttg
     3541 atagtagatt taaaactttg caacagaacc gttcgtcaca tcttcgatca atttctctta
     3601 cgattttctc atccacattt cttattctta cttccatatt aattcctcca atttaaaaag
     3661 gatagcgaaa aggagcctat cctttttgct atcctactac cctcctttta ctatccttca
     3721 ctatcctttc actatcctat ttttaaatat tttgttccca catttatttt tgagaaacgt
     3781 aataggcaca gcctattgga acactttttt taaaaagtgt tcccacgttt ccttatgctc
     3841 tttacaaata aaggctttta gagacgttct ggaattttag attccttgaa ttgttcccat
     3901 tcatcaatca attggctata agtagccatt aatttttttc ttgctcttgt tgctttcgaa
     3961 tcaacaaaaa atcagtttga ttcgttaatt ctaaatattc tgttcggtat tcaatgttca
     4021 tcgtacccgt tttactacat agtaactgat tatgacgttc tttcactttg aataactgct
     4081 ccttctttaa ggtttttaac tccatgccaa ctataagcac aatcctgtat agaggaatca
     4141 ttttcaaacg attctccata attatttatc tttcattgga agttctaaat cactactgtt
     4201 ccaaaaaatg tatggtttgt tttgacgaat attacttcca tctaaccaca attttccgat
     4261 gccatatcgt aggcttgctt cgacttcata aaaattgtag atctcttttc ttcgatctat
     4321 tgctttcatt tcctggccat tttttaaaat agatctgatg cccttaacag tagattcatg
     4381 gatactcgtg tgaacgatat ccgtattgat caattcgtca tatcttccaa aatcaacatt
     4441 gtactcataa ccaagaagtg tcggaatcag gtcaatttca atttcttttt caattgaacg
     4501 tttaataagt ttcgtggata catacccgac aaaaaagaga ataataccca cttcaaacaa
     4561 atacatgtgt ccatttacaa agtgaataaa cgtcaataaa aaatccttca ctccttttgt
     4621 tgttcttcca gattgttcaa tttgttttat taaagtcgct agaattgcat ttacaacaat
     4681 taaatcgaat aggcttttga aaagttttcc tgctgttaat ttttgcctgg taaattgcat
     4741 gactaattga gtaaaaatag acaatagcat gacaaaaatc aacgtcatta gcactgtaaa
     4801 aatgtctgat gctgcaatcc tattgggatc atttatcaag taataagccg ttccaaatac
     4861 tccaatcgag taaacggtta aatcaagaat aagtcgtgcc ccgtaactac aaagttttcc
     4921 taatggccat aattgcaata cccaaaattt ctttcgtttg ctatttcttt tcagaggaag
     4981 ccaggtgtgt tcaatccagt cgtccgattt catttcgatt tcttccctaa attctttcct
     5041 ttaacgtttt cctttgctac tttagcagcg ataggttgct caatgaacca attttttaag
     5101 tacgccgatt gtaaaattaa gctagacaac taaaaaagtc atttgtgcta cactcaaaat
     5161 agttccgtcc aaagaattat aaggagtgag tacaaatgac ctatacccat cttacaacgg
     5221 atgaacttgt aatgatagag tcttatttca aaataaatca atctgttgcg aaaactgcgc
     5281 attgcttgaa tcgttcaaga caaacgatcc ataaagtata cctgttcttc aagcaaggaa
     5341 aatcagcctt agagtactat caacagtata agaaaaacaa atcaaactgt ggtagacgcc
     5401 cgcttgtttt acctgaggaa caatcagaat atattcaaag aaaggttgtt caaggatgga
     5461 cacccgatgt gattgttggt cgtgcagcgt ttcctattcg ttgttctgct cgtaccattt
     5521 atcgtatgtt caaaaagggg ctatttaatc cttctgactt accgatgaaa ggcaagcgta
     5581 aaccaaatgg acatcaagaa agacgtggaa aacaagcttt ccgtcgctct attcatgaac
     5641 gtgaaaaaga ttatagccaa ttctcaaatg agtttggtca ccttgaaggt gacactatcg
     5701 taggtttgaa acataaaagt gctgtaatta ccttagttga gcgattatca aaagttatca
     5761 tcacattgaa accgtgtggt agacaagcga ttgatattga aaacaaatta aatcattggt
     5821 ttgaatctgt accgaaaaat ctattcaaat ccatcacttt tgattgtgga aaggaatttt
     5881 caaattggaa acagatcagt aatgccaatg atattgccat ttatttcgct gatccaggaa
     5941 cgccgtctca aagaggccta aacgagaatt ctaacggatt gttacgtaga gatggtttat
     6001 tgaaatctat ggatttcaat tcagtagatg aatcttttat tcaatctgtc gcatcgaaac
     6061 gaaataatat tcctagaaaa tcactgaatt atcgaacacc tttggaagta tttttgagtt
     6121 acgtaagtat tgatgatttg tctaacttaa tttgacaatt aagagtaaat aaaaactact
     6181 actggatata cttcttcctt tttcttaact cgttagctat tttatactca atatactcct
     6241 gctctaacac acacaaacaa tcatatacct gatccatagt ctccccagaa gctaaggaca
     6301 acccatgaag aaactcaatc ggcaaagaag caactgtttt ttccctggta acccaagaag
     6361 acaaagtccc ctgcttaaaa ccataaagct cacagaacac ttccacagat aatcccaaac
     6421 gaccaatgat aaacacatta acaggatgtg aatagacata attttttaac gacatacaac
     6481 ttcctccatg atacataaga ctgcatacgc agttataagt ataccaccta cacgtagcta
     6541 ggcaaaggga gcaacaccta cagaagtatc atagatatat tgtataaggc aatttgcacc
     6601 ttttattttt caacctgtat ctacttcaaa aagattcact tctttaagag ggttcaaggg
     6661 ggttgataat tgtctaaatg tctcttctac taagctctcg ttgagtccaa atacctctgt
     6721 gatccttgtt gaataatcta gtctcttttc tttacttagt tgaataaccc tcgcgaacaa
     6781 cgttttgact gaagcaatga tcattccgcc atttcttcct cgtgttacct tgtagaagac
     6841 tttattctcc tctcgtaact catttaagat cttatcaagt gttcgcttgg gcatcgttag
     6901 ttgtgaagcc aattcttttt ttgacaggtt caaataaggc tcactagcaa gacttttttc
     6961 gcttagataa gccaatagat cttctttcca ttcattttta tgactatatt ttctttgatc
     7021 tcgtggcttt ttaaatttcc accagccacg tcgttgaatg aacagatcat tattttttaa
     7081 ggttggtgaa acccattgct ggcacaattc aataatgaat tctctgcttg ctgcttgata
     7141 attaccagaa taagcactac gtacaatctt tactaattct gcttcagata gcggttcctg
     7201 taaccgttcg ttgaatgtga acatattgta ttcgcaagta tccaatgcat aacctgaaga
     7261 gtaataagct aaagaaagcg tgaaaatcac attatttcga ccaagacgtc cttctccgcc
     7321 gacgatgttt gctttatgaa gtaacatatc gaaccacggt tcatctactt gacgaatttc
     7381 ttttttctca ggaaagacaa ttagttttcg ttctcgatct tcttttgttt tttgatctga
     7441 ttcaagcttc atcgaccatt gaatccagtc agcaaaagaa tactgatagt tcatttcttt
     7501 aaaaacaata ttattttctt gtgggaatcg agcaattccg aagtggttac atcctagatc
     7561 cactccggac aatttttcag ccaactgtag acgaattgtt tttgaaattt tttttgctac
     7621 atcgatcacc ttgaaattgc ttttctttgt gacatagcct ggtgtcttta gtacaaaata
     7681 aacttgataa ccatgatctg attctaaaat tagcgttggc atgtagccca cttgatctaa
     7741 acaggctaaa ataatttcgc cttgatggtt ctcttttgaa tcgatatcaa ctacaaatgt
     7801 gttgatttga cttaagtttt tctcagaatg acctttaacc actgttcgat aatcatccgc
     7861 ataagtacca aagcgatagg tattaggtgt ccaatgcgtt agtttctttt cgttctctaa
     7921 aatagcttct tccgaagtaa tgaccatacc acgcccaaca ttcattaatt gcttgctacg
     7981 gtacgcaaag atagcaccac tcctactgga atcaatatga cctgcaggtt tcatggtgct
     8041 gtttcgatat ttgtatgtac gtaagccgtt tttaagaata gctgaataaa tttcgctcaa
     8101 catcgattgt aacgtccttt ctcaaacaaa aagagaacac agtttagctt taatctcagc
     8161 catagtatgt gttctctctt cagtgtttaa gaatatttaa tttaagtaca ctaaataaac
     8221 gttcttctgt tgagttgctc gccttttttt ggtagaataa aggcaatcgg aaaaggtatc
     8281 cgatcaactt ttaatataag tagttggtag ctacttacat attcttgaga cgagcgtgaa
     8341 cacatgtcct aaccatgtga ttcagaaata attgattctg ctaaaatcaa ttatttccgt
     8401 ctcttttttt tatgtaattt ttctgtttaa agagtaccac tgcttttgct actttgtaaa
     8461 tagtgaatta tctgtggttt ctatagaatt aagtttacct cttttcactc taaattgtca
     8521 acgcaaaacg cacaaatata tttcgacatt ttgaatatat ctgatatact gaatatagca
     8581 aatatattta gtatatccac tatattcctt ataaagtctg aattcttatc aatttggagt
     8641 atattagata tatcgaatat attaagtata tgaagtatac taaatatatt tagtatagga
     8701 aattggaggg aactatggaa aacttacgga ctgcatttaa aacatcagat aaactaggaa
     8761 aagttgtaat gcttacaggt agaaaaggcg gcattggtaa gactacagac aatgatttat
     8821 tagcgattgt ttctagtcaa ctttttgaaa aagacgtgtt gctaattgat tatgaccaac
     8881 aaagaaatac tacttccaac attggatcaa cgtatcaaat tactagcttt gatcgatcca
     8941 tgtctgcagc aatcaaaaaa ggggactggg tttcaggtat tactcaagta agtccacatt
     9001 tatatattat ggctggctct cctggatctg aagaacttaa tgaatacttg tccgaaaaat
     9061 atcctgatcg tcgtaagaga tcattggcct tcattaaacc tttagaagaa ttgagaaaaa
     9121 atttcgatta tatctttatc gactgcccac catcaacaga taatgtggtt cgtgcattct
     9181 taactgctgc agattatatt attgcaatgc aagaattaaa acggtacgca atggaaggaa
     9241 cagaagattt tattaataaa gttttagttc cgattgtcac gaattttgaa gagtctcatc
     9301 ttcaaatcat tggaattttg cctgttcttt tctcagtgcg acgttcctct caacacgcaa
     9361 actatcaaaa aacaattgat aagtatggtg aatcaaacat ttttaaaagc attgttaaag
     9421 gctcggatcg gttagaaatg tatggtgaga atggtatcca attaaatgat tatgtagatc
     9481 gaagatggtg ggctattttt gcagacatct ttacagaatt agaagaaaga attgaatact
     9541 atgaacaaca tggtgatatt gaaggatttg attatacacc taaatatgct gactcaatgg
     9601 ccaatcatat cctacctctt ggaaaggaga ttaaactaaa tggcattatt accaacgaac
     9661 aaagataaga agaaaattgg tgctgaagat atttcaatta aagatgatat taatgtcgag
     9721 ttaggggaac gaaagtacac tgcgaaagaa agaaaaccag ttcaagtaga tcctcccgta
     9781 ctaaaaacca ttcgggatct tagttatgca aaagatattc ctatgtatga aatagtcaaa
     9841 cttgcaatgg acgcctatgt tgatacttta aacgacgagg aacggattat atataataga
     9901 cgatcaaaag attaaaatat agtaagtata ctagatatag taaatatatt tagtatactt
     9961 actatatttt tagctttatt aatcctaatc cttcaaaata tgtgtattgt ttgggggtat
    10021 acaacatttg gtgttagtat caaatcttag acagcttttc gtagagacaa gaataaatta
    10081 aacttatcta cgagaggatg aatttttatg acccgatatg aagaaaattt taaacaaatg
    10141 attgttgaac tgaatcaaac tggacgttct gttcgagggt tagcgaaaga atatggctta
    10201 tctgaagcaa cgatttacaa atggaagaat ttatatttac ctgatcagtc cacaggactg
    10261 actggaaaag aagtagctga actgagaaaa gaaaatgctc gtttaaatga ggaacttgaa
    10321 atcttaaaaa aagccgcagc catattctct cggaaaacct aaattcgctt gttcaattta
    10381 ttgaaaaatg gtgtaaggat tacactgttt ctttgttgtg tcggttatta gaaatcccta
    10441 gaagtgtcta ttatttttat aagaataaac cgttgacagc tacagaaatc agaaataata
    10501 agttgaaaaa gaagatttca acaatttttt ttacgaataa acaacgctat ggtgccacaa
    10561 agatccatca agttttatta aaagaaggga tttcagtatc tcttaaacat gtccaaaagc
    10621 taatgaaaca attaaattta aggtcgattg tagttaaaaa atatagacct caaaggtcta
    10681 ataaaccgat catggatagg ctacaattaa ctagacagaa aaattaaggt gtgtagacta
    10741 gaagaaaaca taccaggagg aatttttatg tctaagagaa cacgaagaac tttttcacaa
    10801 gaattcaagc aacaaatcgt caatctttac ttagctggaa agccacgtgt agaaatcatt
    10861 cgagaatatg aactaacggc ttcagcattt gacaaatggg taaagcaatt taaaacgagt
    10921 ggttcattca aagaaaaaga taatcttacg cctgaacaaa aagaattgtt agaactacgt
    10981 aaaagaaacc agcaattaga aatggaaaat gatattttaa agcaagcagc gctgatattc
    11041 ggacgaagag acaagtaatc gatgcgaata agcatcttta ccctatatca gcgatgtgca
    11101 gaatattagg tctatcacgt cagtcctatt attatcaatc aaaaccaaag aaagacgaat
    11161 cagaacttga agaagtagtc gctgaagaat ttatccgcag ccgaaaggcc tacggctcaa
    11221 gaaaaataaa aaaagcctta tcaaaacgag gcattcagat cagccgacga aaaattagta
    11281 gaatcatgaa aaatagagga ttaaaatcga gctatactgt tgcttatttt aaagtacatc
    11341 attctacttg caatgaagcc aaaacgacaa acgtattgaa tcgtaaattc ttaagagaca
    11401 acccattaga agcgatcgta acagacttga cttatgtacg agtcgggaaa aaatggaatt
    11461 atgtctgttt cattttggat ctgttcaatc gagaaattct cggctattct tgtggagaac
    11521 ataaagatgc cgttctagta aaaaaagcat ttagccgtat caaacaacct ctgacagagg
    11581 ttgagatttt tcatactgat cgtggaaaag agtttgataa ccaagctatt gatgaattat
    11641 taacaacttt tgacatcaat cgatcattga gtcataaagg ctgtcctttt gataatgccg
    11701 tagctgaatc aacttataag tcgttgaaag tagaatttgt ctatcaatac acatttgaaa
    11761 ccttacaaca attggatttg gagttatttg actatgtcaa ttggtggaac caccttcggt
    11821 tgcacggtac acttggctac gagacaccgg ttggttaccg taaccagaga ttggcgcagc
    11881 gaatccttga taatgagctc ggatgtgcta acgctagcga ggcagtctaa ctttaactgt
    11941 tagctcctgc cgaagatcgt cacatccgag gaggctcatt gtcaaggaca atcggaatag
    12001 catacggaaa gaagttctgc accttataaa atttgtcaaa aaaactgttg ccattccatc
    12061 atttcaaaag agaatctctt aaatcaggac ttttctactg aaacgatctg tgagaaatgg
    12121 gcagctgaca ttacgtacat tcctactaag aaaaatggtt ggtgttactt atcttcaatc
    12181 atggatttac acacgaaaag aattattagt tatacatttt caaaacgaat gactgtggat
    12241 tgtgtcattc aaacattgaa taaagcaaaa atacattatc acattccaga aggaatgatt
    12301 ctacacactg acttgggcag ccaatacaca gcaagagaag tggaacaatg gcttaaaacc
    12361 aacaaaataa ggcattctta tagtagaaaa ggaacacctt atgataatgc tggaatcgaa
    12421 tctttccatg cctcattgaa aaaagaagaa gtctacacga ctagctactc agattttgaa
    12481 gaagcaaatc gagcgctatt tagctacatt gaaggatttt ataaccgaaa tcgaatccat
    12541 agctcgattc actatctaac cccacaggaa tttgaagaat tagcaaaaga aaaaatggct
    12601 taaccgtctc tactaaaatg tgtccaagat attgactcaa atccagtaat atcacaatag
    12661 gaatgctgtt tattatatta gcagcagtgc tgatttttat gatagttatt tggttcttaa
    12721 atgaaagatc taaagacaat caacaccgta gggatatgga gataaaatca atagagtcaa
    12781 tagattattt atcgataata acactaaaaa agccacgttc ctaaacgtga cttacgctga
    12841 acgatatgtg ttgtttggta gacgaacaaa tcgttcatta taaatatatg atggtatcct
    12901 aatatagtaa gcgtggctag cataccttta attactttac cttttacagt taccatacac
    12961 attttttgag cagaaatcaa gtaaataaaa aaatattata tttcacaaca tgctaataga
    13021 ctcatatcta gaacgcatta agtatttacc tgtctggttc gctgagactt cttttttaac
    13081 tttcttcaat tttcttcttt attctaaaaa gcgtactgcg actgaaaccg gttatagctt
    13141 caacttcctt atacgattta cctgaagtaa gcagttcata tgcgtgcctc ttcttcggtg
    13201 tgattgttgc tttcggacgt ccttctctga aattaggatt attctttctt gcaaacattt
    13261 ttccttcttg ggtacgttcc acaatcatat cacgttccat ttctgctaca ctcaacaacg
    13321 tccgtgtcac cattcgcccc atggacgtat tttcaatcgt tcctagattc aaaaccttga
    13381 tcgttacgtt atcatcgagt aaaggttcaa tgatctctaa tgcttctctc gtgttacgag
    13441 caaatcgatc aagcttcgta acaactattt catctccagg cttcagacca gccaacagct
    13501 gattgaattt tggccgatcc cgttttgtac ccgtaaattt ttctgaaaaa atattcttct
    13561 tctctacgcc gttctcaagc aactgttgaa tttgttcttt tgtactttga gaagcggttg
    13621 agacacgtgc atatccatat ttcgtcattt ggtccaccta cttttacaaa tcatctatcg
    13681 tatactcaga atacccttca tagaaatagc tgacatcgat tcctttcata tataactcgc
    13741 gatcgtcaat tttatcagtc agagcattgg aaattagata gcgtatttcc aaatcgttta
    13801 tgggacttcg ctccattgca gaaagatagt ctgctttatc gattaagttc caatctacaa
    13861 ctttttgcaa ttcttccttt aaaatgagat ccagccaaat tctagtactt cggccatttc
    13921 cttctcgaaa tgggtgagcg atattcatct ccacatattt cttgataatc tcttcaaaag
    13981 aagtttgtgg catctgatca atatgctgta acgaatgttc taaatacata actggtgcaa
    14041 atcgaaaatt tccttttgca atattgaccg atcggatttt tccggcaaaa tcgtaaatat
    14101 ccgaaaatag aaattcatga atttcagata aacctttaaa tgtaccaact tcgatttcat
    14161 ttatttttcc acaatcgtac aattctttcg ctttttgttt actgagtttt tcttcaacct
    14221 tagccaactc aacttgattg gttaatccca atttattctc taaaatcatg cgaaatctcc
    14281 ttaactttat gacataagtt tatgaaacac tctcaacccc attatatcgc gattttgttt
    14341 atgtgtcaaa aactattaag tttctgaccc ccgatagatt tttcaagtgt ctctattttg
    14401 cacagtctag ttggttctgt tgcaaagttt taaataaaga ataaaatccc ttacggtatc
    14461 tatgatttaa gctgggattc ccaataatac cttgatttca gtacagaccg aaaacccgaa
    14521 gagagtgcct tcttttcggg ttttcttata taatcctcga atggcttcca tgcctttaat
    14581 cgtggtagag gcagtgcgta aacttcgata gaatttattg cgtctcttta ctggacgatg
    14641 gtcttgttca atcaaattat tcaggtattt aatggtacga tgttctgtcc cttgataaaa
    14701 gccgtattct tttagtttct taaaggcact tgtaatagag ggggctttat ctgtgactac
    14761 aaccttcggt tcatcaaact gcttcactaa ccgcttaaga aaagcatagg ctgcttgtgt
    14821 gtcccgtttt ttacgtaacc aaatatccaa ggttaaacca tctgcatcga tggctcgata
    14881 caaataatgc cattttcctt taattttgat gtacgtttca tccattttcc atgaataaaa
    14941 ggatttttta tttttctttt tccaaatttg atagagtagt ttgccatatt cttgcaccca
    15001 acgataaatc gtcgtatgag aaacgttaat gccacgatca tataagattt cttgaacttc
    15061 acgatagcta aggttataac gaagatagta gcccacggct acaataatca catcctgctg
    15121 aaattgcttt cctttaaaat gattcatcgt cattcctcct gctatctttt tctattattc
    15181 taccttattt gatagtagat ttaaaacttt gcaacagaac cgtgtgaagt atttcagtga
    15241 attattagcc agtagcgaaa ggctcagtgt agatttagaa tcggttatcc agtcctacaa
    15301 ttatggtggt ggtttcttag ggtatgtggc taatcgtgga aataaatata cctttgaact
    15361 ggctcaaagt ttctcaaaag agtattcagg tggcgaaaaa gtgtcttacc ccaatcccat
    15421 agccatacct atcaatgggg gctggcgata caactatggc aatatgtttt atgtgcaact
    15481 ggtaacgcag tatcttgtca caacagagtt tgatgatgat acggtacaag ccatcatgga
    15541 cgaagcactg aaatatgagg gctggcgata cgtttacggt ggagcttccc cgactacttc
    15601 ttttgattgt agcggactga cacaatggac gtatggaaaa gctggaatta acttaccacg
    15661 aaccgcacaa cagcaatatg atgtgaccca gcatatccca ctatcggaag cacaagctgg
    15721 cgatttggtt ttctttcatt ctacctataa cgctggctct tatattactc atgttgggat
    15781 ataccttggc aataaccgta tgtttcatgc aggcgaccca atcggttatg ccgacttaac
    15841 aagcccctac tggcaacagc atttagtggg agcaggacga atcaaacaat gagaaaggaa
    15901 gatttaatga tgaaatttag aaaaaatcag aataaagaaa aacagatacc aaaggaaaag
    15961 aaacctcgtg tctataaggt caatcctcat aaaaaggttg tgattgcctt gtgggtactt
    16021 ttagggctta gtttcagctt tgcgatattc aagcacttta cagctataga tactcatact
    16081 attcacgaaa caactatcat agaaaaggaa tacgttgata ctcatcatgt agaaaatttt
    16141 gtagagaact ttgcgaaagt ctactattca tgggagcaat ccgataagtc cattgataat
    16201 cgaatggaaa gtctaaaagg ctatctgaca gatgaacttc aagctctcaa tgttgataca
    16261 gtacgcaaag atattcctgt atcgtcttct gtaagaggat ttcagatatg gacggtagag
    16321 ccaactggcg acaatgagtt taatgtaacc tacagtgtag accagctcat tacagaggga
    16381 gaaaatacaa agaccgtcca ctctgcttat atagtgagtg tctatgtaga tggttctgga
    16441 aatatggtac tggttaagaa tccgaccatt accaacatac ctaagaaatc aagttataaa
    16501 ccaaaagcca ttgaaagtga ggggacggtt gattccatta caaccaatga aatcaatgag
    16561 tttttaacga cgttcttcaa gctctatcct acagcgacag ccagtgaact ttcctactat
    16621 gtgaatgacg ggatattaaa accaatcgga aaagagtaca tctttcaaga actggtaaat
    16681 cctattcaca atcgtaagga taatcaagtc acggtatcgc tgacagtgga gtatatcgac
    16741 cagcagacca aagcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt
    16801 aattggaaga ttgtaaaata acaaatattg gtacatgatt acagatactt tgtaatcatg
    16861 tactcttttt gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt
    16921 taagtgacta tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc
    16981 aatttgatag caggaacaaa taattagatg tcctttttta ggagggctta gttttttgta
    17041 cccagtttaa gaataccttt atcatgtgat tctaaagtat ccagagaata tctgtatgct
    17101 ttgtatacct atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt
    17161 ttatgccctt ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt
    17221 tttagctcat gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg
    17281 agcgattaca gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt
    17341 agaacgtcag agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac
    17401 gaaggtgaac atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc
    17461 attatcagtt ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca
    17521 aactcgtata ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa
    17581 taagattgac caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact
    17641 ttctatggaa attataatca aacagaaagt agagctgcac cctaatatgt gtgtgatgag
    17701 ctgtacggaa cctgagcaat gggatgtggt aatagaagga aatgatgacc ttttagagaa
    17761 atatatgtcc ggtaaatcat tagaagcatt agaactcgaa caagaggaaa tcagaagatt
    17821 tcagaattgc tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga
    17881 gcagcttata gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga
    17941 actttgcgga aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt
    18001 acgcctttat ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa
    18061 aataaaaatt acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc
    18121 ttattccggg gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg
    18181 agatacaaag ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac
    18241 aactgttgaa ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat
    18301 ctccgacagt gacccgcttc tacaatatta tgtggattct acgacacatg aaattatact
    18361 ttctttctta gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca
    18421 tgtggagata gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc
    18481 agaatatacc attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc
    18541 tgtatcaccg cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata
    18601 cttaaatcaa tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg
    18661 attatatggt tggaatgtga cggattgtaa aatctgtttt aagtatggct tatactatag
    18721 ccctgttagt accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt
    18781 aaaaaaagct ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca
    18841 ggaatatctt tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac
    18901 tcaattgaaa aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga
    18961 atatcgtagt gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa
    19021 agggtaccat gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat
    19081 agataaagta cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa
    19141 tatggtttct tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa
    19201 aggaggaatt aattatgaag tgaaatgaat gtaacagggt tcaattaaaa gagggaagcg
    19261 tatcattaac cctataaact acgtctgccc tcattattgg agggtgaaat gtgaatacat
    19321 cctattcaca atcgaattta cgacacaacc aaattttaat ttggctttgc attttatctt
    19381 tttttagcgt attaaatgaa atggttttga acgtctcatt acctgatatt gcaaatgatt
    19441 ttaataaacc acctgcgagt acaaactggg tgaacacagc ctttatgtta accttttcca
    19501 ttggaacagc tgtatatgga aagctatctg atcaattagg catcaaaagg ttactcctat
    19561 ttggaattat aataaattgt ttcgggtcgg taattgggtt tgttggccat tctttctttt
    19621 ccttacttat tatggctcgt tttattcaag gggctggtgc agctgcattt ccagcactcg
    19681 taatggttgt agttgcgcgc tatattccaa aggaaaatag gggtaaagca tttggtctta
    19741 ttggatcgat agtagccatg ggagaaggag tcggtccagc gattggtgga atgatagccc
    19801 attatattca ttggtcctat cttctactca ttcctatgat aacaattatc actgttccgt
    19861 ttcttatgaa attattaaag aaagaagtaa ggataaaagg tcattttgat atcaaaggaa
    19921 ttatactaat gtctgtaggc attgtatttt ttatgttgtt tacaacatca tatagcattt
    19981 cttttcttat cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc aggaaagtaa
    20041 cagatccttt tgttgatccc ggattaggga aaaatatacc ttttatgatt ggagttcttt
    20101 gtgggggaat tatatttgga acagtagcag ggtttgtctc tatggttcct tatatgatga
    20161 aagatgttca ccagctaagt actgccgaaa tcggaagtgt aattattttc cctggaacaa
    20221 tgagtgtcat tattttcggc tacattggtg ggatacttgt tgatagaaga ggtcctttat
    20281 acgtgttaaa catcggagtt acatttcttt ctgttagctt tttaactgct tcctttcttt
    20341 tagaaacaac atcatggttc atgacaatta taatcgtatt tgttttaggt gggctttcgt
    20401 tcaccaaaac agttatatca acaattgttt caagtagctt gaaacagca
//
