LOCUS       NZ_CP087667            11970 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain DD01845 plasmid
            pDD01845-4, complete sequence.
ACCESSION   NZ_CP087667
VERSION     NZ_CP087667.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22822719
            Assembly: GCF_023066805.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Insertion sequence medicated mrgB disruption is the major mechanism
            of polymyxin resistance in carbapenem-resistant Klebsiella
            pneumoniae isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2021) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023066805.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 04:20:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,970
            CDSs (total)                      :: 5,849
            Genes (coding)                    :: 5,666
            CDSs (with protein)               :: 5,666
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 70 of 183
            Pseudo Genes (incomplete)         :: 125 of 183
            Pseudo Genes (internal stop)      :: 24 of 183
            Pseudo Genes (multiple problems)  :: 35 of 183
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087667.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DD01845"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pDD01845-4"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="39.87 N 116.28 E"
                     /collection_date="2017-12-01"
                     /collected_by="Jiachun Su"
     gene            complement(join(11264..11970,1..25))
                     /gene="mobC"
                     /locus_tag="LMH56_RS29605"
                     /old_locus_tag="LMH56_29605"
     CDS             complement(join(11264..11970,1..25))
                     /gene="mobC"
                     /locus_tag="LMH56_RS29605"
                     /old_locus_tag="LMH56_29605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(25..1989)
                     /locus_tag="LMH56_RS29610"
                     /old_locus_tag="LMH56_29610"
     CDS             complement(25..1989)
                     /locus_tag="LMH56_RS29610"
                     /old_locus_tag="LMH56_29610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3473..3622)
                     /locus_tag="LMH56_RS29615"
                     /old_locus_tag="LMH56_29615"
     CDS             complement(3473..3622)
                     /locus_tag="LMH56_RS29615"
                     /old_locus_tag="LMH56_29615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3707..3964)
                     /locus_tag="LMH56_RS29620"
                     /old_locus_tag="LMH56_29620"
     CDS             complement(3707..3964)
                     /locus_tag="LMH56_RS29620"
                     /old_locus_tag="LMH56_29620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3974..5659)
                     /locus_tag="LMH56_RS29625"
                     /old_locus_tag="LMH56_29625"
     CDS             complement(3974..5659)
                     /locus_tag="LMH56_RS29625"
                     /old_locus_tag="LMH56_29625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(5764..7251)
                     /gene="ltrA"
                     /locus_tag="LMH56_RS29630"
                     /old_locus_tag="LMH56_29630"
     CDS             complement(5764..7251)
                     /gene="ltrA"
                     /locus_tag="LMH56_RS29630"
                     /old_locus_tag="LMH56_29630"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            7896..8141
                     /locus_tag="LMH56_RS29635"
                     /old_locus_tag="LMH56_29635"
     CDS             7896..8141
                     /locus_tag="LMH56_RS29635"
                     /old_locus_tag="LMH56_29635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8415..8786
                     /gene="tolA"
                     /locus_tag="LMH56_RS29640"
                     /old_locus_tag="LMH56_29640"
     CDS             8415..8786
                     /gene="tolA"
                     /locus_tag="LMH56_RS29640"
                     /old_locus_tag="LMH56_29640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            8783..9148
                     /locus_tag="LMH56_RS29645"
                     /old_locus_tag="LMH56_29645"
     CDS             8783..9148
                     /locus_tag="LMH56_RS29645"
                     /old_locus_tag="LMH56_29645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            9323..9601
                     /locus_tag="LMH56_RS29650"
                     /old_locus_tag="LMH56_29650"
     CDS             9323..9601
                     /locus_tag="LMH56_RS29650"
                     /old_locus_tag="LMH56_29650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            9589..9903
                     /locus_tag="LMH56_RS29655"
                     /old_locus_tag="LMH56_29655"
     CDS             9589..9903
                     /locus_tag="LMH56_RS29655"
                     /old_locus_tag="LMH56_29655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            10067..10495
                     /locus_tag="LMH56_RS29660"
                     /old_locus_tag="LMH56_29660"
     CDS             10067..10495
                     /locus_tag="LMH56_RS29660"
                     /old_locus_tag="LMH56_29660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            10521..10700
                     /locus_tag="LMH56_RS29665"
                     /old_locus_tag="LMH56_29665"
     CDS             10521..10700
                     /locus_tag="LMH56_RS29665"
                     /old_locus_tag="LMH56_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(10727..11257)
                     /locus_tag="LMH56_RS29670"
                     /old_locus_tag="LMH56_29670"
     CDS             complement(10727..11257)
                     /locus_tag="LMH56_RS29670"
                     /old_locus_tag="LMH56_29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..10659
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2792..3370
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11268..11968)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.017"
                     /coverage="96.0"
                     /evalue="1.69e-135"
ORIGIN
        1 tgacgttgta ctgctccagt gtcatcagta aagccccgca aaatcatcat ccgcttttgg
       61 tccgccgccg tcggccggag gcaggtactc cgccagcggg tccgttttcg ccagcaccgg
      121 gaaggttttc agcgaaattt cccggcgctc aacccgcagc gggctgctga acgccagccg
      181 cgccacgccc agcccggtca tcaccgcgaa cccgtcaggc aggtgttgca gggtgttcac
      241 gtcaaacagc ggccgcgcct ccttctggat gaagctgtcg ccgctgatgt gctccacgtt
      301 ccccgcctcc cggctggccc ggcggcgctc cacgtccacc cggatctccc cggtctggcc
      361 cgccgcccac tgcgcggact cctggctggc cgcgcgatag aaccagcgga tcggcgtgtt
      421 atccagtacc gtggttttca caaaatcagc gggcagatcc tgcccgcact gaccgaagtc
      481 gcccagcgac tggtgcgcga gcagcaggtt gcagtcccgg ctgcgcagcg tccccagcgc
      541 gttcagcacg tacttcgaca gcagatattt aatctcatcg agcatgatgc tcgcgtgcgg
      601 ccacctccgg aactcatccc gcgcaatgat gatttgcgcg cagcgggcaa acagcatctt
      661 ctgcacccgg atcaccgcct catcgtccat cgagccgatg acgtacaggc agccgccgcc
      721 gttgattatc cccgccacat cgataccgct gtccgtctgc aggacgctca ggtcggccac
      781 gttctccagc ttggtgatca gccccttcac gtcctttttc agggcctccg gcaacgcgta
      841 cgccgccgcc agcacctgct gaatgtttgc cccctgcggg aactgctccg caatcagctt
      901 cgccgctttc tgctcgctga tgcggtagtg gtccgccacg tcgccggtct cactcaggtt
      961 aaacccggca accagcagat tcttcagggc gtactggtcg agatcgcgga acagattcag
     1021 ctgcgccggt ttccccggcc gcagatccag cagggtgaag tccgggcagt gcgcccggaa
     1081 gacgtgcggc gcccacgcgt cccctttggg gtcgaagtac accacagcgt ccccgaaatc
     1141 gcgtacgcag cgaatgagcg cgttggtggc catcgcgctc ttgccgctcc cgggcaggcc
     1201 gaggatctga atattggtct tgcgccaggt atcacgaggg accagcaccg gctggccctg
     1261 ctcatcctta cccagaaaca ccgcctgctg tttccgggcc tgctccgtgg aaaaatcggc
     1321 cagcgcgtta accggtaccg gctccccgaa atgcaggtgg cggacgtctg tcatttctcc
     1381 ggtccgctgg ctgctgcgcc ggctctcctg attcagtttc gcgttcaccg cctcgtcagc
     1441 ggccgcaaaa tgaccatcaa gcagcaggaa cgccagcagc cgcgccagaa ggacgccggc
     1501 gacaagccct ccggcacacc aggctatccc gaacaggtta gccggctgca gcaggtaata
     1561 atctgcgttc gcatagaggt ccgggcgcag gacggtttct ccccagctct cgataaacac
     1621 ggcgacaacc agccccccca gaagggccag accggcagcg ccggtcagcc agtacgcgta
     1681 gttccagaca tgtcgccgtg ccctgaccat ccgcccgggt gaatccagcg aggccagcac
     1741 cgaaaatggc acagtccggt tattatcgcg cttatagcga tcggcatact cccgccgcgc
     1801 cttatggctc agccccacaa aacgcattcg gccggcccag agttcagcca gaccgaaaaa
     1861 caggccgata accagaaaaa aaccggccag actgtaatac tcccggtaca gccagctgac
     1921 ccacgggtaa atctgccggg ccagctccgg gatatgccac tgccaggcaa gcgaatccgt
     1981 attaaacatg caatctctct ccttcagacg acgggtttac cggcgtgatg cgcagccccc
     2041 gctgcacaag ccgcggacat gccccaaggc atggcggcgg cggtcacgtc cgtggtgatt
     2101 gcgttcagct ttcaccgcgg aggccagcct ctttccgccc catgcggaaa aggcgcagcc
     2161 ccggaggaac aacgagcacg ccccagcgtg tgagaggaaa aggcggcacc ccagtgcggt
     2221 cttttccggt gttccggccc ccgtcagagt tcgccatagc gagcggctga ccacccgcaa
     2281 aatcaggagc agctttgctg cttcgtcagg tgcgtccagc accgtggatt tttgtgggtg
     2341 gtcaggcgtg agcctgacgg gggccgggtg aatttctggc gtggccggcg tccagccgga
     2401 cagagccaga aagaggggaa aagaccgtgc gtcggtggcg tccagccagc gtaagcaagg
     2461 tcttttcccc tgaagtcagc cacgccccag cggggatgac ttgcatttct ccgcaaggag
     2521 aaaaaagcaa cagagatagg ttaacgtaaa atgttcagcg aaaaaccgac atggttcacc
     2581 tatcctgata attgatcgtc agggaataga aagacttaat caggggacaa tatcccacat
     2641 cagcgcgtcc ggtgaccgtc ttgcaggtgc cggggaaatt gcgcctcctc gctcactcgg
     2701 tcgctacgct ccgggcgtga gactgcggcg ggcgctgcgg acacatacaa agttacccac
     2761 agattccgtg gataagcagg ggactaacat gtgaggcaaa acggcagggc cgcgccggtg
     2821 gcgtttttcc ataggctccg ccctcctgcc agagttcaca taaacagacg ctttttcggt
     2881 gcatctgtgg gagccgtgag gctcaaccat gaatctgaca gtacgggcga aacccgacag
     2941 gacttaaaga tccccaccgt ttccggtagg tcgctccctc ttgcgctctc ctgttccgac
     3001 cctgccgttt accggatacc tgtccgccgt tctcccttgc gggaagtgtg gcgctttctc
     3061 atagctcaca cactggtatc tcggctcggt gtaggtcgtt cgctccaagc tgggctgtaa
     3121 gcaagaactc cccgttcagc ccgactgctg cgccttatcc ggtaactgtt cacttgagtc
     3181 caacccggga aagcacgcaa aacgccactg gcagcagcca ttggtaactg ggagttcgca
     3241 gaggatttgt ttagctaaac acgcggttgc tcttgaagtg tgcgccaaag tccggctaca
     3301 ctggaaggac agatttggtt gctgtgctct gcgaaagcca gttaccacgg ttaagcagtt
     3361 ccccaactga cttaaccttc gatcaaacca cctccccagg tggttttttc gtttacaggg
     3421 caaaagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctactgaa
     3481 ccgcgatccc cgtcagttca gaggaggacg atggtgccac cgttccgccc tgaacatccc
     3541 ggatataatt tgcctgacat gcggccagaa gaaagccagc aacaatcaat ataaaaagaa
     3601 aaatagtctt tacctttttc atatactttc cctgtattta aagactccgg cattcatccc
     3661 tgaacaccgg aagtcagtat ttcaaatcat ccttgataat tttttgttac cagttaccgt
     3721 cccgataatc aaaagctata taacaattat ccacatcagg ttttatttga tttttaaaaa
     3781 atggctgtag taaaggaacc cattctttct tcaaatcaaa ctcaccatta ttaatattat
     3841 cctttaaagg cattcccaag ctttcaatga cagaaccatc gtcaccaaag tctttagaat
     3901 actcttcccc tttaaactct tcagttttct tatcaaacca tgtcaaacgt aatttaagcc
     3961 ccatatttac ctctcaaaga tattttttaa tgttccgttt tggatctggc cctttaacct
     4021 gcttacccgt tttgggatca aatgcgccta ggtgttcgcc atcactggca cgataccctt
     4081 caagctcacc gtgctgggaa tcccattcat aaattttacg ttttttatcg ccataccatc
     4141 gggcccgctt accaccacca ccttgtttag gtgttttagg atcacctcgc tttaactctc
     4201 caagtccctt tatgtcttct gttttaggta ctggatgata atcatggccg tagtctttag
     4261 tccccttgcg aggcttgttc ttttcctcat ttaacttatt ttctgcatcc ttcgcttttt
     4321 tctccttttg tttccggctc tccatcgccc cggtaagtgc agccttatct ctggataagg
     4381 attcattcat tgcgtttaaa gatgcctgag ccgcttttaa tttctcattc tgattctgaa
     4441 tatccttatc aagatagcga acctgatcct gccacccgga gccatattca tgaggcgggt
     4501 tataagtcac aaaatcatct ttatttgcct ctgcctcttt taccttttta tcggcttcct
     4561 gaacagcagc ccttgcaccg gggagcgcct tctctgtcga tgcaatgcgg ctgtttaagg
     4621 tcgcaatatt tttatcttcg gcatccagct cagctttcgc tttatcatag tctcttttca
     4681 gcccctcttc cggatgagcg gcgtcccatg cctgctggcg acgcttttct tcctcctgac
     4741 gctgttttac ctgtgccggg gtaagaacat ctgtcaccga cacataaacc ggcttctgtc
     4801 cggtctcttt cgggaaacga ataacggcct cacgggagtt accaccggct gtaaaaccag
     4861 ccggacgtga atcacctttt tcagcaacaa tgccttttgg cggggctttc gctgtcggaa
     4921 cacctttcgg tacgctcacc tgcagagacg ggagacccgg aatcgacaca ctgaataccc
     4981 ccggacgttt tgtcggtttc gcatccacca cagggacact cattggccga cctgcgacaa
     5041 ccgcaatatg ctgccgctca tccttcacca catccacaac ccgttgtctg acacgaaccg
     5101 tcgcctggtc cagtggtaaa gtacttgccg gagtctccgt caccgcatcg gccggtaatg
     5161 acgtcataat ttttgacatc attttcggat catcttttgc aatctcagaa ggcagcacac
     5221 cgtagatcgc aatcccccac agaccaaact taaacggccc cttcagggca gccagcacat
     5281 cagcaacggc ggccgacaac gcatcgccgg aaactgataa agccagtcct tcagcccccg
     5341 gagtagccag ggcaggcaga ccgaaggcca tcgcagtggc cgaagactgt ccgccaccag
     5401 agctaccgct atgattgccg ctgttaccac cattaccacc gggtttactc ccgccgttac
     5461 caaactcccc cggattatag ttatgaatat ctccattcgg cgtatgcgtc gtaccccagc
     5521 ccggcccgct gtttggatcc gtaccaccac ctgatgaagg gccaccttta ccagacgtac
     5581 cgttcacatt cccactggcc tgaccaccat tatgtcccag acctgaatta cccggacctc
     5641 gaccgtctcc accactcata ccgttttcct ctttgacatt taaaacaata aattaaagcc
     5701 aacatgtcgc ggtagggata cccgttaccg gatacccccc gcacagatcc cggcgtgcgc
     5761 gatttacgca ccgggctcct gcctcgggtg tctggcggtg aaccgctcca caggccatgg
     5821 atgaagaacc cgaacccttg gtagccatgc ggctgccagt ttgtttgctt tcgtccaggt
     5881 cgtatcatcc ttctggctcc tgcgcctgag cgcccggcgc cagaggtttg ttacgtgtgt
     5941 cctgaacttc tgcatggtgg ggaagttgcc cggtaccgag tgatagttca ggtatccctg
     6001 aaccactctc ctgagccatt ttccctgttc ggggattgag taatgccagc gccttcgcag
     6061 accgtctttg atggctttca gagttgccgt catccgatcc cggcgggtct ttcgtatcag
     6121 catgaacctg ccgttgcgat ctttcccgct gatgtgcgtg aacccgagga agttgaacgt
     6181 ttctggtttg ccttttcccc tgatggcacg gttttcggca gcgaagcggc cgaactccat
     6241 cagacgggtt ttctccgggt gaaccgtgag tccgaactcc ctcagtctgc gctgcatggc
     6301 tatacggaag cgccgggcat cgtatcgttt gtcgaacccg atgacgatgt catcggcgta
     6361 tctgaccatt accacattgc ctgtggcata gcgacgtcgc cactgatgcg cccacagatc
     6421 gaagacgtag tggaggtata tgtttgccag cagcggtgag atgaccgcac cctgtggggt
     6481 gccttcctcc gttgctcgcc attgaccctc ctccgacgtc ccggctgtga gccacttacg
     6541 tatgagcctg attaccctcc ggtcgccgat ccgatgctct gtgaacctga tcagccattc
     6601 gtggctcacc ctgtcgaaga actgactgat gtcggcatcc agtacccagt ttacgttagt
     6661 gcgtaccagc cctgtggcca gtgcgtccag tgcatcgtgc tggcttcgcc cgggtctgaa
     6721 cccgtatgag aaccccataa agtcgttttc atagactgcg ttcaggattt tcaccagcgc
     6781 atactggacg atcttgtcct ccagcgaggc gatgccgagc gggcgttgtt ttccatccgc
     6841 ttttgggatg tagtgacgcc tgccgggctg cgccctgtag ctgccctgat gtagcctccg
     6901 gtgcagatct gttatgttgt tcttcatgtt tccggcgtag tccatccacc tgatgccatc
     6961 cactccggcg gccgctttcc tgctcaggga gaggaatgcg gcttccagtg cttcgactgt
     7021 cagcaggtgg aacaatgctg taaaccgttc tttcttccgc tgcttcgcag cttcccgcac
     7081 gcgtgacagc ctctgtgaca tgctttcccg gctctgtgtc cggcgcatgt gtggctgttc
     7141 cgcgttcccc ttggccccgc tccttcgctc cactgactcc gctcctttcg ggttgttcgc
     7201 ctgcttcgcc gctactatga gcgagtccga cttctcctct ccgtacatca ccggctatga
     7261 ctcctcgtct tcccggtgcg ggccatctcc gacactggca gatggtcaga ggggagatct
     7321 cccggttccc gcgtagagat cgtattgaca tgccagggtc tcagaccccg ccgggtccat
     7381 gtggcactcg cagtgtcgca ccctatgatg ttgccttccg ttaacagtac aacgtcggca
     7441 cccggtaatt taatatacat ttcgtggctc aatggctggc ctgtcaacac ccctgtcaac
     7501 gcttcgcccc atacctcgcg gtatgcaacg catgactcgg ggaccttgtg gattgctggt
     7561 ccttcaatgg tcggggactt tcaccccttg atctctaacc ggtctcccgg cgcacactgt
     7621 acatatacac agtataatta ttaccttact cctcccgttt tcaggtgtca atatatgaga
     7681 ttagttctca tcttcaggca caaaaaagcg cccggagcca cctgtaagca gatcgcgtct
     7741 tttcccgaaa atttctcatg gcaacaaatt ttattattta agacattcat cacacttaca
     7801 tttcagatgg tatagacaca tctgaaagct aactggcatg aggagaggac ctgcggttaa
     7861 actgttcggg aaacctgaga cgcccggagg ccactatgac gcacctgaaa ctgactcccg
     7921 ctgaagaaaa gcgcctggcg gacgcctttg tcgctcaggc actggccatg ggcctgaaag
     7981 ggaacaaaac gctgccaaag gcggtaaaac aacaggtgcg ggaaagggtg cttcgtgcca
     8041 ttcaggacga acgtgaagcc aggcgggcgc aggaaatgcg tcgggagaaa tacgaggcta
     8101 cggaaaccac cttcacctgg cagccacagc gccgccggta aggggtattt accataacgt
     8161 cgcattgccc gcaccgctca gacggtctga gggcgtttcc ggcggctacg ggataaaaca
     8221 ggcaacaaac cacccggaaa accgccggaa acgcgcccgg taaattgtaa cccgctgcat
     8281 gcctatgcac gcaactgcat ggcgggccgt ttggtgtaaa aacgatacga taagccgtgt
     8341 ttatgtaaat gaacgtggcc gtaaagagtg acaaatatac atgtaacatt ttttactgac
     8401 aggtcatcaa cccgatgcgt ttaataccct ttatcctgct ggcattgtgc agccccttac
     8461 tcctgacggc ctgtaagcat tccaccaccc agccgccgaa aatttatact ctggataagt
     8521 catctaaaat cagtagctac gctgcggcca tcaagaccgc tgttgaaatg cagatgtttg
     8581 aaccagaggc ctatgcggga aaaatgtgca ccgtgcggat atccatacaa cgtgatggca
     8641 ctctgaacag cgccattgct gaaggaggag atccgaaact ctgtaaagca gcaatatcag
     8701 ccgttaccca cgccaaaatt ccgccggcac cggatgaaaa tacctggcaa atattcaaaa
     8761 acgcccctct ggatttcaga ccatgaaagc ttttactctg acagtgctgg cggccatgtt
     8821 cctcactccg gtacatgcga taccactaaa acccgtaacc acactgcagc caacctaccc
     8881 ggtacaggcc gcagcaaaac gcatcgaagg tcaggtgacc gccacgtttg atattctgga
     8941 cgatggacga gtggacaacg tgtcgcttac cggctgtcac cttttttttc gggaaacgct
     9001 gacggcaatg aggcactggc tatatcccgc agggcacccg gcaatgcgac aggttataaa
     9061 ttttgaattc agtctgaaaa atgacggctt acaggatccg cgaccagtca ccacccaaaa
     9121 gctcaatgat gatggttgcc gccagtaaca ggtcctttaa cccgcaggct gcaatagtcc
     9181 tggatgatga gtgagcctgt agcagccctg attagtggaa gcgaagctga aaaatgggca
     9241 gcaaaagccg tatttctggc aaatagcgcc gccacggatt aaagtagtca tgtgtaatac
     9301 atatgactac aaaggggctt ttatgtcatc agcaacatcc atcaggctcg atgaagaact
     9361 taaggaccgg ctgaaaacgc tggccgatga ccggcaccgc tcagcgcacg cgctaatgct
     9421 ggaggcaatc accgaataca tcgaccggga agagaagcgc agccagtacc tgcgggacgg
     9481 acaggccgca tggcagcact atcaggaaac gggtctgcac cttactgcgg aagaagcgga
     9541 agcctggata agtacctggg gaactgagaa cgagcaggat gctccgccat gccacaggta
     9601 acgatttccg cgctggcaca gcgcgattta caacgccttc aggactttct caaaaccaaa
     9661 aatcggctgg cagccagaaa aggaggtgag gtgatcgtcc gggctatcca gcaactgaaa
     9721 acattgccag acattggccg cccggtgccg tttctgccgc tggaatataa ggaactggtg
     9781 atcgggtttg gcgacagtgg ctatgtgatg ctctaccgcc acgaccggga aatggaccag
     9841 attgtgatcg tcaccgtcag gcatcagaaa gaatccgggt atccgggggc agacagcctc
     9901 taataaccga tgacgggggc gcaattcatt gcgtggccgt cgccggtttg acaagcgcag
     9961 cgcgtcagaa aaccacatcc tgaacggaaa aacgggtaaa attcagcgat acatttaccg
    10021 tgagaaatta cacacctttc acacccgata aaatcagggg tatacagtgg cgaaaacatt
    10081 gctcaacctt tcacaggcag cacaagccgc gggcattacc agaagaacgc tatacaatca
    10141 tgtaaaacag ggaaaggtta ccgtgtcacg tgacgggaaa aacaaccctg tcgtggatgt
    10201 gtcagaactg atccgtgtat acggaaatgt gaatatccct gagaaacaaa ttcccggcat
    10261 ttcacaccgt gaaaatacac aaaaaaattt cccacaggaa cagcttctgg caatgcaaaa
    10321 ggaactggcc gatctccggc aggctgtcac cctgatgctg gaagataaaa catcccggga
    10381 agaagaacgc cgccagcacg atgatgagcg ccggaaactc caggccgaag tggacagatt
    10441 gacaacagaa ctgacacaga agaaaaaacg cttctggtcc ggctggttta gctgagaact
    10501 taccgaaggg aatggagaac atgacgaagc aggaaaaaac tgccctcaat atggctaaat
    10561 tcattaagga tcagtcgctg ctgttgctgg aaaagctgaa cgagctggac atggataccc
    10621 cggccgacat gtgcgaacgg cttcacgagg atgcggaaac actctggcag gtgatgcggg
    10681 ataagctcgg ggaagaataa cagcaccggc catgccggtg ctggcgttat tttttgccga
    10741 acggccacca ccggcgggcg ggtttgtcct ccggcagttc aggcaccggc acgttaaaat
    10801 agcggctgtt atattcccgc atcagctccg ctttcagctt ctcgttcacc gcgctttccc
    10861 cgtgccgctc gctgaccacc tcaatcatcg cgttaatctg ctccggtgag cacttcccgg
    10921 catcaaccac ctgcacaccg ttctcgatgg cgaaccgctc cacctttcga tagaggtaat
    10981 cacacagctt cattcctgtg ggtttgtccc gttcccattg cggattttca ccggtgaaaa
    11041 agtacgtggc tttttcgtcg ctgttgagcc gctcgtcata catcccgatg gatttcatca
    11101 tcgccagatg ctgggcccgg tcgacaaaac agaaaacata cgccccggtc tgccaggcga
    11161 gcaccaccat gtcttccaga tgctcgctgg ttggcataat gcagcgctcg ccgtctttgt
    11221 cttccacgtc ctcaataaat accatgtaca gcgccataat ccctcactcc gttgtcataa
    11281 atttaaagca cgcgaacagc ctctcccggt ccagctccac cgttttgcgg gcctcgccgg
    11341 tctgtggatt cacgatttca tccaccgcca gaatcgcccg ctgtacccgg acaagcgtcg
    11401 gccggtcagg gcagatgtac cagatttcat cgatgccgtt cgcccggcgg ttgaacagat
    11461 acccggacac cacttcctga taccgcctgc ggctcttgac ggtgcgctct acctcaaatg
    11521 ccaccgttct gccgtccagc tccgccagcg cgtccggcac tttcatccct ttcagggcca
    11581 tacggtgcag gtaccgccag ccggtggccc cccggccctc catggagagg cgcagggact
    11641 gcacggccag ccggtgcggc agctgcgccg ccgacaccct tgacggctga aacggaataa
    11701 cgtccgtcag cggctcgtcc tcgtcgaacg agaacgccac cccgtgcggc gtcagccccc
    11761 acacgctgac ctgacggccg tcaaccgggt atttcgcccg gaccagcagc cctttcgcca
    11821 ccagactgtc caggaagcgg taaaccggct gtaccgccgc aatccccagc cgtcgcgcaa
    11881 ccagctccgc cgtggaacac agctcttccc gcagccagcg cagggtcagc cgtgttttct
    11941 ccgcgttccg cgcctgccgc ttcgcgtggc
//
