LOCUS       NZ_CP087662             5596 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain DD01635 plasmid
            pDD01635-5, complete sequence.
ACCESSION   NZ_CP087662
VERSION     NZ_CP087662.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22822720
            Assembly: GCF_023066785.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Insertion sequence medicated mrgB disruption is the major mechanism
            of polymyxin resistance in carbapenem-resistant Klebsiella
            pneumoniae isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2021) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023066785.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 04:21:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,042
            CDSs (total)                      :: 5,920
            Genes (coding)                    :: 5,727
            CDSs (with protein)               :: 5,727
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 193
            CDSs (without protein)            :: 193
            Pseudo Genes (ambiguous residues) :: 0 of 193
            Pseudo Genes (frameshifted)       :: 70 of 193
            Pseudo Genes (incomplete)         :: 136 of 193
            Pseudo Genes (internal stop)      :: 23 of 193
            Pseudo Genes (multiple problems)  :: 35 of 193
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087662.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DD01635"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pDD01635-5"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="39.87 N 116.28 E"
                     /collection_date="2017-12-01"
                     /collected_by="Jiachun Su"
     gene            complement(join(5562..5596,1..190))
                     /locus_tag="LMH51_RS30030"
                     /old_locus_tag="LMH51_30030"
     CDS             complement(join(5562..5596,1..190))
                     /locus_tag="LMH51_RS30030"
                     /old_locus_tag="LMH51_30030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(857..1069)
                     /locus_tag="LMH51_RS30035"
                     /old_locus_tag="LMH51_30035"
     CDS             complement(857..1069)
                     /locus_tag="LMH51_RS30035"
                     /old_locus_tag="LMH51_30035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1076..1585)
                     /locus_tag="LMH51_RS30040"
                     /old_locus_tag="LMH51_30040"
     CDS             complement(1076..1585)
                     /locus_tag="LMH51_RS30040"
                     /old_locus_tag="LMH51_30040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1620..2324)
                     /locus_tag="LMH51_RS30270"
                     /pseudo=""
     CDS             complement(<1620..2324)
                     /locus_tag="LMH51_RS30270"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2263..2607)
                     /locus_tag="LMH51_RS30050"
                     /old_locus_tag="LMH51_30050"
     CDS             complement(2263..2607)
                     /locus_tag="LMH51_RS30050"
                     /old_locus_tag="LMH51_30050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2597..2773)
                     /locus_tag="LMH51_RS30055"
                     /old_locus_tag="LMH51_30055"
     CDS             complement(2597..2773)
                     /locus_tag="LMH51_RS30055"
                     /old_locus_tag="LMH51_30055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3413..3571
                     /locus_tag="LMH51_RS30275"
     CDS             3413..3571
                     /locus_tag="LMH51_RS30275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            3929..4255
                     /locus_tag="LMH51_RS30060"
                     /old_locus_tag="LMH51_30060"
     CDS             3929..4255
                     /locus_tag="LMH51_RS30060"
                     /old_locus_tag="LMH51_30060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            4281..4826
                     /locus_tag="LMH51_RS30065"
                     /old_locus_tag="LMH51_30065"
     CDS             4281..4826
                     /locus_tag="LMH51_RS30065"
                     /old_locus_tag="LMH51_30065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            4892..5242
                     /locus_tag="LMH51_RS30070"
                     /old_locus_tag="LMH51_30070"
     CDS             4892..5242
                     /locus_tag="LMH51_RS30070"
                     /old_locus_tag="LMH51_30070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(5312..5578)
                     /locus_tag="LMH51_RS30075"
                     /old_locus_tag="LMH51_30075"
     CDS             complement(5312..5578)
                     /locus_tag="LMH51_RS30075"
                     /old_locus_tag="LMH51_30075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          complement(2860..2936)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          3125..3522
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(742..2273)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cagacgaatg cactgcttca tcaccacggc gaacgcgtgc agcagtttct gctgaaagat
       61 aataatcctc caggtcttcc agatgcgcca gtattgcttc ccgcgcataa aacgtctttg
      121 ttctgccggt ttgcttcgcc agcgagtcca gacgggactc aatctcatca gataaccgga
      181 tagcaagcat ataaacctcc cgaacaccat ttgctataca tgtatatcaa cagtaccaca
      241 atcaacctaa aagaacaaaa aataatcttg ttagcccctc ttgataatcc gtaacgcggt
      301 caatatatct agcttgaggg ctttcctctt aactattgaa aggataacga ttatgggtct
      361 taagccgggt ccgaaacgaa ttgccgattc aacaggggaa cctgataaac gccagcgtga
      421 caataaaaaa acgccgggaa atactgacaa attgaaaccc agcaaatctt caaaaaaata
      481 agtcagtagt taaaagccct ccggggcttt tttgtatctg ccgttcacgc tgattaaaca
      541 taacacccgt tgcccgcacc gccccggacg ggttcgccgc gaacggggtg gcagtcaggt
      601 aaaaccggca acaaaccacc cgataaaccg ccccgttcgc gacagataaa ttttacctgg
      661 ctgcatagcc atgcagccag gtgaatgaca tccggtttca gcgtaaatgc ccgatgagaa
      721 ccctgttttc ctgcatttca gcccagcgac ggaccgtcat agcttcgctg ccgggaacgc
      781 gcttccgtca gttccttttc gtgctgccgt tgctcgtgcg cccgttctgc ggccacggcc
      841 atctcatgac gctgcactac cggctcaaac gctgcagttg cccgctcaag cgttccacct
      901 gctgactcaa gcgcgtcacg cgctcgctca gcgccgcgtt ctcccgttgc ataagccctg
      961 acattttccg ccattccccg aaggcgctct cccactcgtc cagccttttc gagtagtcct
     1021 gctgtagctg ctctaatgcg ctcagcaact gtttttccag ctcggtcatc ggcacttact
     1081 ccttcaccag tctcagcgcg ttctgcttcg accgcccgtt cccgaccgtc cagctctgcg
     1141 tcctgtctac ggccgtcccc ttcggcagca ccagaaaacg tccgttgctg tcttccaggt
     1201 acgtcacgcc ccaggtcttc gcgttcagct tcgacagcgc tgcgttctgc cggtcgattt
     1261 cggccagatt tgacgctatc aagctcccct gataccacaa caccccgctc aggctggcga
     1321 acagcagtcc tgacaccatc gtgatggtca gccatgtccg cccgaccata cgcatcacgc
     1381 tcaggcggtt cgactgcatg gccgcgctca taccctgctc gtgggcgcgg atggcggcgc
     1441 tgattctctt cccgctttcg ctcagttccg ctctgacaga cttctcgtgc tcgctgaatg
     1501 cggctttcag catttcgccg gtactctgct gctgcgcttt cgatttctgc tctaagtcct
     1561 tcgccagcgt taaaagactg ttcatagatg gctcccttaa gtcggatgtt tcgccctccg
     1621 tccgggtcgg caatgctgat gctgctcttt gtggtgcgca cgacctcaaa acctgcgctt
     1681 tccagcgcct cagtgacgtc ctgacgcgtt ttaagctccc ctgacgaggc gagggccagt
     1741 aagccccgcg taatagcctc tgcggcttcc tgcttcgttt tgggtaacga agatggcgtc
     1801 accagcgcgc gccggttctc cggtgcgttc gggtcgtgca gccccagtcg cccgttcacc
     1861 acggtctgcc acgcatcgat gcgcggacgg tctgcccggt cgtagtacgg ctggagacgc
     1921 ctgccggtca gcagttccgt gttcggtacc agaaaattca gttccagccg ccccttgtcc
     1981 cggtgttcaa cccacagcac gctgtactgg tctttatcga gtccgggcat cagaacccgc
     2041 tcgaagctcg ccatcagctt ttcacgctgg ccgggcggta aatcctgttc agcaaaagac
     2101 aggacgccgg aggtgtactt tttggcgtag ggcgaggcgt cgataagctc ccggacctcc
     2161 tccggctttc cctgcaggac gatggccccg tcgcgctggc ggtctttccc cagcagataa
     2221 tccaccggcc cggcaccgcc gcctcgccct cgcggatgaa acttaacgat catcgtctgc
     2281 ccccttttcc agcacggcat gccgcagccg ctcgagttcg gcatcaatgg ccatcagcgc
     2341 ggccaccacc tgaacccggt cagcgcctga ccactggccg ccgttaattt tccgggcgat
     2401 ctggttgagg ttgttcccca tcccggcaag ctggcgcagc aaaaccggat cgatggacgg
     2461 cagccgcaac gaccgcgccg ggcgcgtatc gagacaggtt tcccgcatcc acaccgccag
     2521 ttgccttcca ttgcagcgct ccagcagctg ctggtactcc ccatcggtca cccggatacc
     2581 taaaaaacgg gtacgcttaa ccgccatcct ctcctccgat atccgcaaaa tgtgaccggg
     2641 ctgaacgctc aagctctgcc atctgctccc ggcggtaatc ttcctcctgt gcctgacgca
     2701 tcagatttgc catcactgcc ggatgccagg atcttttttt tacgacgagc ggcttcttct
     2761 tcggcttctg cattatccct ccttcgcaaa cgccggacgg cacggaaaag ttttccgggt
     2821 cgctccggcg ttggacaagc gccagcgcgt cagtggtgtt agcgggtttc ggggcgcagc
     2881 cctgaaccag tcacagcgtt tacgctgtgt atactggctt actatgttgg catgagagca
     2941 cgattcatga aagtgcctca tgtggcaggt gtgatatgtg gctcaggagc gtccgcaata
     3001 ccatgcacgg acagatgaaa tgacgcctcc tcgctcactc ggtagctgcg ctcctgccgt
     3061 tcggctgcgg cgggcgtgac cgactcacgg ataacgggat aaagcaaagg cgctcaaatc
     3121 agaaagggcc ggagaaaagg atgcggcgtt gccgtttttc cataggctcc gcctcccctg
     3181 acgagcatca cagaaatgga cgctcaagtc agaggaggac gaaacccgac aggacttaaa
     3241 gatgccggtg atttctctcc ggcggctccc tcgtgcgctc tcctgttccg accctgccgt
     3301 ttaccggata cctgttccgc ctttttccct ttcgggaagc gtggcgcttt ctcatagctc
     3361 acgctgctgg tatctcggct cggtttaggt cgttcgctcc aagctgggct gtatgcacga
     3421 accccccgtt cagcccgact gctgcgccct ttccggtaac tatcatcttg agtccaaccc
     3481 ggacggacac gcaaaaacgc cactggcagc agccattggt aactggatat atacgagaga
     3541 gatattgaga ggtcttgaag tgggtgccta actgcggcta cactgaaaga acagttttgg
     3601 tatctgcggt ctcgtatacc cagttacccg gttaagcagt tccccaactg acttaacctt
     3661 cgatcaaacc gcctccccag gcggtttttt cgtttacagt gcaagagatt acgcgcagaa
     3721 aaaaaggatc tcaagaagat cctttaaatg atcgtcagtt ctgttatccc cggagctcca
     3781 tccatcccac ggaaaaacac ccgccactga tgccctcagc gcactgcaca tcatgtacaa
     3841 accactccct ccctcctttt ttattgcgtt tcttaatgcg caatggtagc tttgtgcgca
     3901 acaaaaaacg caatagcaag ggggggtaat gagagaaaga acaaatatca tgctagatat
     3961 aggattaaag caatccttac aacggctttc gaataaaacc aaaatgagtc taagtgatct
     4021 tattaaccag agattatcag gttcactttc agttgaagaa cgcattggcg aaaagcctga
     4081 atggatgaga aaccttgatc cctatctcgc caatcacttt tcaaatattg agttaccgct
     4141 cccttcacca ttaaaagaat accaagaaaa aatggatgag atagattcaa ttattaatga
     4201 catgatggtt ctaaaggaat atttcagagc taagttttac gacgaaataa aataggcacg
     4261 ctatacggag gcggagaatt atgtttttta aacggtattt aacggagaag gaaatataca
     4321 aggcagcact cccaattgtt aggcttcaag ggagagttaa cacttcatta cgttttttat
     4381 ccaaatactc agatgtaaac aaaaatggat taattgcatt gcatcttaat ttattacatt
     4441 ttatctcata caacatacac ggtgtagagt ctctgaaaat cactcaagca ggcataaaca
     4501 aagctgtaca acaaagccct cacgcagatg aaataaaaaa tattgcgcag gcaataggac
     4561 gcaaaggacc tgagtacatc gaaggattaa ttagaaaccc atgggggaag aaaaatgata
     4621 atccactaat atttataatg agcattgaag aaagtataaa agaaaccctt aactcaggtt
     4681 atatgttata tagagtagct cccataactg agggtgaagc agctgctttc atggatgttc
     4741 ttaaacaatt attattattt gaggataatt ttcgcctttt tggtagagac ttggtttata
     4801 aaatagaaaa attaaaaatc agataacaaa gtatggtgaa cgaaaaatga aagagaggat
     4861 agtgaaatgt gtaataccaa gattctcaac tatgtattgg ttaattgcgt tcctagcatt
     4921 tatattcccc ggtcgatctt ttgctgacgg ttacaccgct tactaccgat gtaatggtac
     4981 tcaggaagca tctcttactg ttaaagataa caaactaagt atggttgtag gtctttctaa
     5041 gtttaatagg caactggata cttatagatt caaagaaaag ggaatcgaag tagtaatgca
     5101 gagagctgct tctgatgata actctgaggc cataatgtct tttattatgc ctgacgttaa
     5161 caatatgacc ttattagtta aaaataacgg ctacattatc gttaagacaa cctgctggtt
     5221 aactaaaacc gacaaacagt agaacacgta gttagtcgag tatttaccat aatacctgtt
     5281 gtcggcatca aaaaagcccg catagcgggc ttcagtcgta aacttcgcgg cgatgtccaa
     5341 tcgtggcggc aaggataaca agctcgtcat ctcggatctc acacagaacg cgataatctc
     5401 ccacccgata gcgccagaac tcgcccagct cacctttgag cggcttccct gactggcgag
     5461 gatcggcagc aactgcaatg cgtaaactca taaaatccac aatccgtcgt gcgttctgtt
     5521 tgtccatctt gcgtaacgat ttgagcgccc gatcggaata gttaatcgtc cagaccaagt
     5581 gacttcctca cgtctt
//
